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PMC:3245220 / 8087-10511 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T5134 0-5 P09603 denotes M-CSF
T5135 2-5 P04141 denotes CSF
T5136 148-153 P09603 denotes M-CSF
T5137 150-153 P04141 denotes CSF
T5138 253-258 P09603 denotes M-CSF
T5139 255-258 P04141 denotes CSF
T5140 409-414 P09603 denotes M-CSF
T5141 411-414 P04141 denotes CSF
T5142 590-595 P09603 denotes M-CSF
T5143 592-595 P04141 denotes CSF
T5144 938-943 P09603 denotes M-CSF
T5145 940-943 P04141 denotes CSF
T5146 2016-2021 P09603 denotes M-CSF
T5147 2018-2021 P04141 denotes CSF
T5148 2215-2220 P09603 denotes M-CSF
T5149 2217-2220 P04141 denotes CSF
T5150 2364-2369 P09603 denotes M-CSF
T5151 2366-2369 P04141 denotes CSF
T23146 1001-1006 P09603 denotes M-CSF
T23147 1003-1006 P04141 denotes CSF
T23148 1128-1133 P09603 denotes M-CSF
T23149 1130-1133 P04141 denotes CSF
T23150 1389-1394 P09603 denotes M-CSF
T23151 1391-1394 P04141 denotes CSF
T23152 1842-1847 P09603 denotes M-CSF
T23153 1844-1847 P04141 denotes CSF

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T5152 2250-2259 Positive_regulation denotes increased
T5153 2275-2283 Positive_regulation denotes activity
T5154 2260-2265 Protein denotes NF-κB
T5155 0-5 Protein denotes M-CSF
T5156 148-153 Protein denotes M-CSF
T5157 253-258 Protein denotes M-CSF
T5158 409-414 Protein denotes M-CSF
T5159 590-595 Protein denotes M-CSF
T5160 938-943 Protein denotes M-CSF
T5161 2016-2021 Protein denotes M-CSF
T5162 2215-2220 Protein denotes M-CSF
T5163 2364-2369 Protein denotes M-CSF
T23154 1001-1006 Protein denotes M-CSF
T23155 1128-1133 Protein denotes M-CSF
T23156 1389-1394 Protein denotes M-CSF
T23157 1842-1847 Protein denotes M-CSF
R3885 T5153 T5152 themeOf activity,increased
R3886 T5154 T5153 themeOf NF-κB,activity
R3887 T5162 T5152 causeOf M-CSF,increased

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T5264 0-5 Protein denotes M-CSF
T5265 14-19 Protein denotes NF-κB
T5266 148-153 Protein denotes M-CSF
T5267 162-171 Protein denotes NF-κB DNA
T5268 253-258 Protein denotes M-CSF
T5269 172-179 Binding denotes binding
T5270 154-161 Positive_regulation denotes induced
T5271 307-320 Protein denotes nuclear NF-κB
T5272 336-341 Binding denotes bound
T5273 409-414 Protein denotes M-CSF
T5274 429-434 Protein denotes NF-κB
T5275 439-446 Binding denotes binding
T5276 423-428 Regulation denotes alter
T5277 516-538 Protein denotes pNF-κB-SEAP constructs
T5278 555-560 Protein denotes NF-κB
T5279 590-595 Protein denotes M-CSF
T5280 666-678 Protein denotes SEAP release
T5281 487-499 Gene_expression denotes transfecting
T5282 654-662 Positive_regulation denotes increase
T5283 782-786 Protein denotes pTAL
T5284 787-791 Protein denotes SEAP
T5285 810-829 Protein denotes NF-κB binding sites
T5286 802-809 Negative_regulation denotes lacking
T5287 867-886 Protein denotes pTAL-SEAP construct
T5288 903-907 Protein denotes SEAP
T5289 938-943 Protein denotes M-CSF
T5290 846-857 Gene_expression denotes transfected
T5291 846-857 Positive_regulation denotes transfected
T5292 895-902 Gene_expression denotes produce
T5293 2016-2021 Protein denotes M-CSF
T5294 2030-2035 Protein denotes NF-κB
T5295 2022-2029 Positive_regulation denotes induced
T5296 2140-2145 Protein denotes NF-κB
T5297 2146-2150 Protein denotes SEAP
T5298 2260-2274 Protein denotes NF-κB reporter
T5299 2250-2259 Positive_regulation denotes increased
T5300 2364-2369 Protein denotes M-CSF
T5301 2378-2383 Protein denotes NF-κB
T23195 1001-1006 Protein denotes M-CSF
T23196 1015-1020 Protein denotes NF-κB
T23197 1007-1014 Positive_regulation denotes induces
T23198 1128-1133 Protein denotes M-CSF
T23199 1156-1161 Protein denotes NF-κB
T23200 1166-1173 Binding denotes binding
T23201 1348-1370 Protein denotes pNF-κB-SEAP constructs
T23202 1389-1394 Protein denotes M-CSF
T23203 1471-1476 Protein denotes NF-κB
T23204 1526-1530 Protein denotes SEAP
T23205 1599-1603 Protein denotes SEAP
T23206 1531-1539 Localization denotes secreted
T23207 1531-1539 Localization denotes secreted
T23208 1587-1595 Positive_regulation denotes increase
T23209 1730-1733 Protein denotes pNF
T23210 1734-1736 Protein denotes κB
T23211 1737-1741 Protein denotes SEAP
T23212 1824-1847 Protein denotes mouse recombinant M-CSF
R3907 T5266 T5270 causeOf M-CSF,induced
R3908 T5267 T5269 themeOf NF-κB DNA,binding
R3909 T5269 T5270 themeOf binding,induced
R3910 T5271 T5272 themeOf nuclear NF-κB,bound
R3911 T5273 T5276 causeOf M-CSF,alter
R3912 T5274 T5275 themeOf NF-κB,binding
R3913 T5275 T5276 themeOf binding,alter
R3914 T5277 T5281 themeOf pNF-κB-SEAP constructs,transfecting
R3915 T5280 T5282 themeOf SEAP release,increase
R3916 T5285 T5286 themeOf NF-κB binding sites,lacking
R3917 T5287 T5290 themeOf pTAL-SEAP construct,transfected
R3918 T5288 T5292 themeOf SEAP,produce
R3919 T5290 T5291 themeOf transfected,transfected
R3920 T5293 T5295 causeOf M-CSF,induced
R3921 T5294 T5295 themeOf NF-κB,induced
R3922 T5298 T5299 themeOf NF-κB reporter,increased
R17695 T23195 T23197 causeOf M-CSF,induces
R17696 T23196 T23197 themeOf NF-κB,induces
R17697 T23199 T23200 themeOf NF-κB,binding
R17698 T23203 T23206 themeOf NF-κB,secreted
R17699 T23204 T23207 themeOf SEAP,secreted
R17700 T23205 T23208 themeOf SEAP,increase

events-check-again

Id Subject Object Predicate Lexical cue
T5252 0-5 Protein denotes M-CSF
T5253 148-153 Protein denotes M-CSF
T5254 253-258 Protein denotes M-CSF
T5255 409-414 Protein denotes M-CSF
T5256 590-595 Protein denotes M-CSF
T5257 938-943 Protein denotes M-CSF
T5258 2016-2021 Protein denotes M-CSF
T5259 2215-2220 Protein denotes M-CSF
T5260 2250-2259 Positive_regulation denotes increased
T5261 2260-2265 Protein denotes NF-κB
T5262 2275-2283 Positive_regulation denotes activity
T5263 2364-2369 Protein denotes M-CSF
T23191 1001-1006 Protein denotes M-CSF
T23192 1128-1133 Protein denotes M-CSF
T23193 1389-1394 Protein denotes M-CSF
T23194 1842-1847 Protein denotes M-CSF
R3904 T5259 T5260 causeOf M-CSF,increased
R3905 T5261 T5262 themeOf NF-κB,activity
R3906 T5262 T5260 themeOf activity,increased

GO-CC

Id Subject Object Predicate Lexical cue
T5184 111-115 http://purl.obolibrary.org/obo/GO_0005623 denotes Cell
T5185 625-630 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5186 2101-2106 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5187 2244-2249 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T5188 2321-2326 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T23169 1656-1661 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T23170 1677-1682 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

GO-MF

Id Subject Object Predicate Lexical cue
T5176 168-179 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T5177 435-446 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T5178 172-179 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T5179 439-446 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T5180 571-578 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T5181 816-823 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T5182 810-823 http://purl.obolibrary.org/obo/GO_0051059 denotes NF-κB binding
T5183 2022-2044 http://purl.obolibrary.org/obo/GO_0004704 denotes induced NF-κB activity
T23167 1162-1173 http://purl.obolibrary.org/obo/GO_0003677 denotes DNA binding
T23168 1166-1173 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-BP

Id Subject Object Predicate Lexical cue
T5174 2022-2044 http://purl.obolibrary.org/obo/GO_0004704 denotes induced NF-κB activity
T5175 2384-2399 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional
T23166 1021-1036 http://purl.obolibrary.org/obo/GO_0006351 denotes transcriptional

UBERON-AE

Id Subject Object Predicate Lexical cue
T22809 1764-1769 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4910 0-5 NN denotes M-CSF
T4911 6-13 VB denotes Induces
T4912 14-19 NN denotes NF-κB
T4913 20-35 JJ denotes Transcriptional
T4914 36-44 NN denotes Activity
T4915 45-47 IN denotes in
T4916 48-53 JJ denotes Human
T4917 54-70 NNP denotes Monocyte–Derived
T4918 71-82 NN denotes Macrophages
T4919 83-84 -LRB- denotes (
T4920 84-88 NN denotes MDMs
T4921 88-89 -RRB- denotes )
T4922 90-93 CC denotes and
T4923 94-99 NNP denotes Mouse
T4924 100-110 NNP denotes Macrophage
T4925 111-115 NNP denotes Cell
T4926 116-120 NNP denotes Line
T4927 120-121 -COMMA- denotes ,
T4928 122-125 NN denotes RAW
T4929 126-131 CD denotes 264.7
T4930 132-134 TO denotes To
T4931 135-144 VB denotes determine
T4932 145-147 IN denotes if
T4933 148-153 NN denotes M-CSF
T4934 154-161 VB denotes induced
T4935 162-167 NN denotes NF-κB
T4936 168-171 NN denotes DNA
T4937 172-179 NN denotes binding
T4938 180-182 IN denotes in
T4939 183-188 JJ denotes human
T4940 189-200 NN denotes macrophages
T4941 200-201 -COMMA- denotes ,
T4942 202-204 PRP denotes we
T4943 205-214 VB denotes performed
T4944 215-219 NN denotes EMSA
T4945 220-228 NN denotes analysis
T4946 229-231 IN denotes on
T4947 232-239 JJ denotes nuclear
T4948 240-247 NN denotes lysates
T4949 248-252 IN denotes from
T4950 253-266 JJ denotes M-CSF-treated
T4951 267-272 NN denotes MDMs.
T4952 273-280 JJ denotes Similar
T4953 281-283 TO denotes to
T4954 284-292 JJ denotes previous
T4955 293-300 NN denotes reports
T4956 301-302 -LRB- denotes [
T4957 302-304 CD denotes 11
T4958 304-305 -RRB- denotes ]
T4959 305-306 -COMMA- denotes ,
T4960 307-314 JJ denotes nuclear
T4961 315-320 NN denotes NF-κB
T4962 321-335 RB denotes constitutively
T4963 336-341 VB denotes bound
T4964 342-345 NN denotes DNA
T4965 346-348 IN denotes in
T4966 349-363 JJ denotes non-stimulated
T4967 364-373 NN denotes monocytes
T4968 374-375 -LRB- denotes (
T4969 375-381 NN denotes Figure
T4970 382-384 NN denotes 1A
T4971 384-385 -RRB- denotes )
T4972 387-400 RB denotes Interestingly
T4973 400-401 -COMMA- denotes ,
T4974 402-408 VB denotes adding
T4975 409-414 NN denotes M-CSF
T4976 415-418 VB denotes did
T4977 419-422 RB denotes not
T4978 423-428 VB denotes alter
T4979 429-434 NN denotes NF-κB
T4980 435-438 NN denotes DNA
T4981 439-446 NN denotes binding
T4982 447-449 IN denotes by
T4983 450-454 NN denotes EMSA
T4984 456-458 IN denotes In
T4985 459-467 NN denotes contrast
T4986 467-468 -COMMA- denotes ,
T4987 469-474 IN denotes after
T4988 475-486 RB denotes transiently
T4989 487-499 VB denotes transfecting
T4990 500-505 JJ denotes human
T4991 506-510 NN denotes MDMs
T4992 511-515 IN denotes with
T4993 516-527 NN denotes pNF-κB-SEAP
T4994 528-538 NN denotes constructs
T4995 539-549 VB denotes containing
T4996 550-554 CD denotes four
T4997 555-560 NN denotes NF-κB
T4998 561-570 NN denotes consensus
T4999 571-578 NN denotes binding
T5000 579-588 NN denotes sequences
T5001 588-589 -COMMA- denotes ,
T5002 590-595 NN denotes M-CSF
T5003 596-605 NN denotes treatment
T5004 606-608 IN denotes of
T5005 609-612 DT denotes the
T5006 613-624 VB denotes transfected
T5007 625-630 NN denotes cells
T5008 631-639 VB denotes resulted
T5009 640-642 IN denotes in
T5010 643-644 DT denotes a
T5011 645-653 JJ denotes 2.3-fold
T5012 654-662 NN denotes increase
T5013 663-665 IN denotes in
T5014 666-670 NN denotes SEAP
T5015 671-678 NN denotes release
T5016 679-681 IN denotes in
T5017 682-685 DT denotes the
T5018 686-693 NN denotes culture
T5019 694-699 NN denotes media
T5020 700-708 VB denotes compared
T5021 709-711 TO denotes to
T5022 712-715 NNP denotes PBS
T5023 716-717 -LRB- denotes (
T5024 717-724 NN denotes vehicle
T5025 724-725 -RRB- denotes )
T5026 725-733 JJ denotes -treated
T5027 734-745 VB denotes transfected
T5028 746-750 NN denotes MDMs
T5029 751-752 -LRB- denotes (
T5030 752-758 NN denotes Figure
T5031 759-761 NN denotes 1B
T5032 761-762 -RRB- denotes )
T5033 764-766 IN denotes As
T5034 767-768 DT denotes a
T5035 769-776 NN denotes control
T5036 776-777 -COMMA- denotes ,
T5037 778-781 DT denotes the
T5038 782-791 NN denotes pTAL-SEAP
T5039 792-801 NN denotes construct
T5040 802-809 VB denotes lacking
T5041 810-815 NN denotes NF-κB
T5042 816-823 NN denotes binding
T5043 824-829 NN denotes sites
T5044 830-833 VB denotes was
T5045 834-838 VB denotes used
T5046 840-845 NN denotes Cells
T5047 846-857 VB denotes transfected
T5048 858-862 IN denotes with
T5049 863-866 DT denotes the
T5050 867-876 NN denotes pTAL-SEAP
T5051 877-886 NN denotes construct
T5052 887-890 VB denotes did
T5053 891-894 RB denotes not
T5054 895-902 VB denotes produce
T5055 903-907 NN denotes SEAP
T5056 908-910 IN denotes in
T5057 911-914 DT denotes the
T5058 915-922 NN denotes absence
T5059 923-925 CC denotes or
T5060 926-934 NN denotes presence
T5061 935-937 IN denotes of
T5062 938-943 NN denotes M-CSF
T5063 944-945 -LRB- denotes (
T5064 945-951 NN denotes Figure
T5065 952-954 NN denotes 1B
T5066 954-955 -RRB- denotes )
T5067 1987-1989 PRP denotes We
T5068 1990-1994 RB denotes next
T5069 1995-2007 VB denotes investigated
T5070 2008-2015 IN denotes whether
T5071 2016-2021 NN denotes M-CSF
T5072 2022-2029 VB denotes induced
T5073 2030-2035 NN denotes NF-κB
T5074 2036-2044 NN denotes activity
T5075 2045-2047 IN denotes in
T5076 2048-2051 DT denotes the
T5077 2052-2057 NN denotes mouse
T5078 2058-2068 NN denotes macrophage
T5079 2069-2073 NN denotes cell
T5080 2074-2078 NN denotes line
T5081 2078-2079 -COMMA- denotes ,
T5082 2080-2083 NN denotes RAW
T5083 2084-2089 CD denotes 264.7
T5084 2091-2094 NN denotes RAW
T5085 2095-2100 CD denotes 264.7
T5086 2101-2106 NN denotes cells
T5087 2107-2111 VB denotes were
T5088 2112-2123 VB denotes transfected
T5089 2124-2128 IN denotes with
T5090 2129-2135 CC denotes either
T5091 2136-2139 DT denotes the
T5092 2140-2150 NN denotes NF-κB-SEAP
T5093 2151-2159 NN denotes reporter
T5094 2160-2162 CC denotes or
T5095 2163-2170 NN denotes control
T5096 2171-2180 NN denotes pTAL-SEAP
T5097 2181-2190 NN denotes construct
T5098 2192-2194 IN denotes As
T5099 2195-2200 VB denotes shown
T5100 2201-2203 IN denotes in
T5101 2204-2210 NN denotes Figure
T5102 2211-2213 NN denotes 1C
T5103 2213-2214 -COMMA- denotes ,
T5104 2215-2220 NN denotes M-CSF
T5105 2221-2230 NN denotes treatment
T5106 2231-2233 IN denotes of
T5107 2234-2237 NN denotes RAW
T5108 2238-2243 CD denotes 264.7
T5109 2244-2249 NN denotes cells
T5110 2250-2259 VB denotes increased
T5111 2260-2265 NN denotes NF-κB
T5112 2266-2274 NN denotes reporter
T5113 2275-2283 NN denotes activity
T5114 2284-2286 IN denotes by
T5115 2287-2295 RB denotes 2.5-fold
T5116 2296-2300 IN denotes over
T5117 2301-2305 DT denotes that
T5118 2306-2308 IN denotes of
T5119 2309-2320 JJ denotes non-treated
T5120 2321-2326 NN denotes cells
T5121 2328-2336 RB denotes Together
T5122 2336-2337 -COMMA- denotes ,
T5123 2338-2341 PRP-DOLLAR- denotes our
T5124 2342-2346 NN denotes data
T5125 2347-2358 VB denotes demonstrate
T5126 2359-2363 IN denotes that
T5127 2364-2369 NN denotes M-CSF
T5128 2370-2377 VB denotes induced
T5129 2378-2383 NN denotes NF-κB
T5130 2384-2399 JJ denotes transcriptional
T5131 2400-2408 NN denotes activity
T5132 2409-2411 IN denotes in
T5133 2412-2423 NN denotes macrophages
T22987 1001-1006 NN denotes M-CSF
T22988 1007-1014 VB denotes induces
T22989 1015-1020 NN denotes NF-κB
T22990 1021-1036 JJ denotes transcriptional
T22991 1037-1045 NN denotes activity
T22992 1046-1048 IN denotes in
T22993 1049-1060 NN denotes macrophages
T22994 1062-1063 -LRB- denotes (
T22995 1063-1064 NN denotes A
T22996 1064-1065 -RRB- denotes )
T22997 1066-1072 NN denotes Nuclei
T22998 1073-1077 VB denotes were
T22999 1078-1087 VB denotes extracted
T23000 1088-1092 IN denotes from
T23001 1093-1098 JJ denotes human
T23002 1099-1103 NN denotes MDMs
T23003 1104-1111 VB denotes treated
T23004 1112-1119 IN denotes without
T23005 1120-1122 CC denotes or
T23006 1123-1127 IN denotes with
T23007 1128-1133 NN denotes M-CSF
T23008 1134-1137 IN denotes for
T23009 1138-1140 CD denotes 15
T23010 1141-1143 CC denotes or
T23011 1144-1146 CD denotes 30
T23012 1147-1154 NN denotes minutes
T23013 1156-1161 NN denotes NF-κB
T23014 1162-1165 NN denotes DNA
T23015 1166-1173 NN denotes binding
T23016 1174-1182 NN denotes activity
T23017 1183-1186 VB denotes was
T23018 1187-1195 VB denotes analyzed
T23019 1196-1198 IN denotes by
T23020 1199-1203 NNP denotes EMSA
T23021 1204-1209 NNP denotes Shown
T23022 1210-1212 VB denotes is
T23023 1213-1214 DT denotes a
T23024 1215-1229 JJ denotes representative
T23025 1230-1234 NN denotes blot
T23026 1235-1239 IN denotes from
T23027 1240-1245 CD denotes three
T23028 1246-1257 JJ denotes independent
T23029 1258-1269 NN denotes experiments
T23030 1271-1273 NN denotes NS
T23031 1273-1274 -COLON- denotes :
T23032 1275-1290 JJ denotes non-stimulated.
T23033 1291-1292 -LRB- denotes (
T23034 1292-1293 NN denotes B
T23035 1293-1294 -RRB- denotes )
T23036 1295-1300 JJ denotes Human
T23037 1301-1305 NN denotes MDMs
T23038 1306-1317 RB denotes transiently
T23039 1318-1329 VB denotes transfected
T23040 1330-1334 IN denotes with
T23041 1335-1344 NN denotes pTAL-SEAP
T23042 1345-1347 CC denotes or
T23043 1348-1359 NN denotes pNF-κB-SEAP
T23044 1360-1370 NN denotes constructs
T23045 1371-1375 VB denotes were
T23046 1376-1383 VB denotes treated
T23047 1384-1388 IN denotes with
T23048 1389-1394 NN denotes M-CSF
T23049 1395-1397 IN denotes in
T23050 1398-1404 JJ denotes X-vivo
T23051 1405-1411 NN denotes medium
T23052 1412-1415 CC denotes and
T23053 1416-1425 VB denotes incubated
T23054 1426-1429 IN denotes for
T23055 1430-1431 CD denotes 6
T23056 1432-1437 NN denotes hours
T23057 1438-1444 IN denotes before
T23058 1445-1448 DT denotes the
T23059 1449-1459 NN denotes collection
T23060 1460-1462 IN denotes of
T23061 1463-1469 NN denotes medium
T23062 1471-1476 NN denotes NF-κB
T23063 1477-1485 NN denotes activity
T23064 1486-1489 VB denotes was
T23065 1490-1498 VB denotes analyzed
T23066 1499-1501 IN denotes by
T23067 1502-1511 VB denotes measuring
T23068 1512-1515 DT denotes the
T23069 1516-1522 NN denotes amount
T23070 1523-1525 IN denotes of
T23071 1526-1530 NN denotes SEAP
T23072 1531-1539 VB denotes secreted
T23073 1540-1544 IN denotes into
T23074 1545-1548 DT denotes the
T23075 1549-1555 NN denotes medium
T23076 1556-1559 CC denotes and
T23077 1560-1564 NN denotes data
T23078 1565-1568 VB denotes are
T23079 1569-1578 VB denotes expressed
T23080 1579-1581 IN denotes as
T23081 1582-1586 JJ denotes fold
T23082 1587-1595 NN denotes increase
T23083 1596-1598 IN denotes of
T23084 1599-1603 NN denotes SEAP
T23085 1604-1612 NN denotes activity
T23086 1613-1617 IN denotes over
T23087 1618-1622 DT denotes that
T23088 1623-1625 IN denotes in
T23089 1626-1635 NN denotes pTAL-SEAP
T23090 1636-1647 VB denotes transfected
T23091 1648-1655 VB denotes resting
T23092 1656-1662 NN denotes cells.
T23093 1663-1664 -LRB- denotes (
T23094 1664-1665 NN denotes C
T23095 1665-1666 -RRB- denotes )
T23096 1667-1670 NN denotes RAW
T23097 1671-1676 CD denotes 264.7
T23098 1677-1682 NN denotes cells
T23099 1683-1687 VB denotes were
T23100 1688-1699 RB denotes transiently
T23101 1700-1711 VB denotes transfected
T23102 1712-1716 IN denotes with
T23103 1717-1726 NN denotes pTAL-SEAP
T23104 1727-1729 CC denotes or
T23105 1730-1741 NN denotes pNF-κB-SEAP
T23106 1742-1751 NN denotes construct
T23107 1753-1758 NN denotes Cells
T23108 1759-1763 VB denotes were
T23109 1764-1769 NN denotes serum
T23110 1770-1777 VB denotes starved
T23111 1778-1781 IN denotes for
T23112 1782-1783 CD denotes 4
T23113 1784-1789 NN denotes hours
T23114 1790-1795 RB denotes prior
T23115 1796-1798 TO denotes to
T23116 1799-1800 CD denotes 2
T23117 1801-1806 NN denotes hours
T23118 1807-1818 NN denotes stimulation
T23119 1819-1823 IN denotes with
T23120 1824-1829 NN denotes mouse
T23121 1830-1841 JJ denotes recombinant
T23122 1842-1847 NN denotes M-CSF
T23123 1848-1849 -LRB- denotes (
T23124 1849-1852 CD denotes 100
T23125 1853-1858 NN denotes ng/ml
T23126 1858-1859 -RRB- denotes )
T23127 1861-1868 NN denotes Culture
T23128 1869-1874 NN denotes media
T23129 1875-1878 VB denotes was
T23130 1879-1888 VB denotes collected
T23131 1889-1891 TO denotes to
T23132 1892-1899 VB denotes measure
T23133 1900-1904 NN denotes SEAP
T23134 1905-1915 NN denotes production
T23135 1917-1921 NN denotes Data
T23136 1922-1925 VB denotes are
T23137 1926-1935 VB denotes expressed
T23138 1936-1940 JJ denotes mean
T23139 1941-1942 JJ denotes ±
T23140 1943-1948 NN denotes S.E.M
T23141 1948-1949 -COMMA- denotes ,
T23142 1950-1953 IN denotes for
T23143 1954-1959 CD denotes three
T23144 1960-1971 JJ denotes independent
T23145 1972-1983 NN denotes experiments
R3665 T4910 T4911 arg1Of M-CSF,Induces
R3666 T4914 T4911 arg2Of Activity,Induces
R3667 T4914 T4912 arg1Of Activity,NF-κB
R3668 T4914 T4913 arg1Of Activity,Transcriptional
R3669 T4914 T4915 arg1Of Activity,in
R3670 T4918 T4916 arg1Of Macrophages,Human
R3671 T4918 T4917 arg1Of Macrophages,Monocyte–Derived
R3672 T4918 T4919 arg1Of Macrophages,(
R3673 T4918 T4922 arg1Of Macrophages,and
R3674 T4920 T4919 arg2Of MDMs,(
R3675 T4921 T4919 arg3Of ),(
R3676 T4922 T4915 arg2Of and,in
R3677 T4926 T4922 arg2Of Line,and
R3678 T4926 T4923 arg1Of Line,Mouse
R3679 T4926 T4924 arg1Of Line,Macrophage
R3680 T4926 T4925 arg1Of Line,Cell
R3681 T4926 T4927 arg1Of Line,","
R3682 T4928 T4927 arg2Of RAW,","
R3683 T4928 T4929 arg1Of RAW,264.7
R3684 T4931 T4930 arg1Of determine,To
R3685 T4933 T4934 arg1Of M-CSF,induced
R3686 T4934 T4931 arg2Of induced,determine
R3687 T4934 T4932 arg1Of induced,if
R3688 T4934 T4938 arg1Of induced,in
R3689 T4937 T4934 arg2Of binding,induced
R3690 T4937 T4935 arg1Of binding,NF-κB
R3691 T4937 T4936 arg1Of binding,DNA
R3692 T4940 T4938 arg2Of macrophages,in
R3693 T4940 T4939 arg1Of macrophages,human
R3694 T4942 T4931 arg1Of we,determine
R3695 T4942 T4943 arg1Of we,performed
R3696 T4943 T4930 modOf performed,To
R3697 T4943 T4941 arg1Of performed,","
R3698 T4945 T4943 arg2Of analysis,performed
R3699 T4945 T4944 arg1Of analysis,EMSA
R3700 T4945 T4946 arg1Of analysis,on
R3701 T4948 T4946 arg2Of lysates,on
R3702 T4948 T4947 arg1Of lysates,nuclear
R3703 T4948 T4949 arg1Of lysates,from
R3704 T4948 T4952 arg1Of lysates,Similar
R3705 T4948 T4959 arg1Of lysates,","
R3706 T4951 T4949 arg2Of MDMs.,from
R3707 T4951 T4950 arg1Of MDMs.,M-CSF-treated
R3708 T4952 T4953 arg1Of Similar,to
R3709 T4955 T4953 arg2Of reports,to
R3710 T4955 T4954 arg1Of reports,previous
R3711 T4955 T4956 arg1Of reports,[
R3712 T4957 T4956 arg2Of 11,[
R3713 T4958 T4956 arg3Of ],[
R3714 T4961 T4960 arg1Of NF-κB,nuclear
R3715 T4961 T4963 arg1Of NF-κB,bound
R3716 T4963 T4959 arg2Of bound,","
R3717 T4963 T4962 arg1Of bound,constitutively
R3718 T4963 T4965 arg1Of bound,in
R3719 T4964 T4963 arg2Of DNA,bound
R3720 T4967 T4965 arg2Of monocytes,in
R3721 T4967 T4966 arg1Of monocytes,non-stimulated
R3722 T4967 T4968 arg1Of monocytes,(
R3723 T4970 T4968 arg2Of 1A,(
R3724 T4970 T4969 arg1Of 1A,Figure
R3725 T4971 T4968 arg3Of ),(
R3726 T4975 T4974 arg1Of M-CSF,adding
R3727 T4975 T4976 arg1Of M-CSF,did
R3728 T4975 T4978 arg1Of M-CSF,alter
R3729 T4978 T4972 arg1Of alter,Interestingly
R3730 T4978 T4973 arg1Of alter,","
R3731 T4978 T4976 arg2Of alter,did
R3732 T4978 T4977 arg1Of alter,not
R3733 T4978 T4982 arg1Of alter,by
R3734 T4981 T4978 arg2Of binding,alter
R3735 T4981 T4979 arg1Of binding,NF-κB
R3736 T4981 T4980 arg1Of binding,DNA
R3737 T4983 T4982 arg2Of EMSA,by
R3738 T4985 T4984 arg2Of contrast,In
R3739 T4989 T4987 arg2Of transfecting,after
R3740 T4989 T4988 arg1Of transfecting,transiently
R3741 T4989 T4992 arg1Of transfecting,with
R3742 T4991 T4989 arg2Of MDMs,transfecting
R3743 T4991 T4990 arg1Of MDMs,human
R3744 T4994 T4992 arg2Of constructs,with
R3745 T4994 T4993 arg1Of constructs,pNF-κB-SEAP
R3746 T4994 T4995 arg1Of constructs,containing
R3747 T5000 T4995 arg2Of sequences,containing
R3748 T5000 T4996 arg1Of sequences,four
R3749 T5000 T4997 arg1Of sequences,NF-κB
R3750 T5000 T4998 arg1Of sequences,consensus
R3751 T5000 T4999 arg1Of sequences,binding
R3752 T5003 T4989 arg1Of treatment,transfecting
R3753 T5003 T5002 arg1Of treatment,M-CSF
R3754 T5003 T5004 arg1Of treatment,of
R3755 T5003 T5008 arg1Of treatment,resulted
R3756 T5007 T5004 arg2Of cells,of
R3757 T5007 T5005 arg1Of cells,the
R3758 T5007 T5006 arg2Of cells,transfected
R3759 T5008 T4984 arg1Of resulted,In
R3760 T5008 T4986 arg1Of resulted,","
R3761 T5008 T4987 arg1Of resulted,after
R3762 T5008 T5001 arg1Of resulted,","
R3763 T5008 T5009 arg1Of resulted,in
R3764 T5008 T5020 arg1Of resulted,compared
R3765 T5012 T5009 arg2Of increase,in
R3766 T5012 T5010 arg1Of increase,a
R3767 T5012 T5011 arg1Of increase,2.3-fold
R3768 T5012 T5013 arg1Of increase,in
R3769 T5012 T5016 arg1Of increase,in
R3770 T5015 T5013 arg2Of release,in
R3771 T5015 T5014 arg1Of release,SEAP
R3772 T5019 T5016 arg2Of media,in
R3773 T5019 T5017 arg1Of media,the
R3774 T5019 T5018 arg1Of media,culture
R3775 T5021 T5020 arg2Of to,compared
R3776 T5024 T5023 arg2Of vehicle,(
R3777 T5025 T5023 arg3Of ),(
R3778 T5028 T5021 arg2Of MDMs,to
R3779 T5028 T5022 arg1Of MDMs,PBS
R3780 T5028 T5023 arg1Of MDMs,(
R3781 T5028 T5026 arg1Of MDMs,-treated
R3782 T5028 T5027 arg2Of MDMs,transfected
R3783 T5028 T5029 arg1Of MDMs,(
R3784 T5031 T5029 arg2Of 1B,(
R3785 T5031 T5030 arg1Of 1B,Figure
R3786 T5032 T5029 arg3Of ),(
R3787 T5035 T5033 arg2Of control,As
R3788 T5035 T5034 arg1Of control,a
R3789 T5039 T5037 arg1Of construct,the
R3790 T5039 T5038 arg1Of construct,pTAL-SEAP
R3791 T5039 T5040 arg1Of construct,lacking
R3792 T5039 T5044 arg1Of construct,was
R3793 T5039 T5045 arg2Of construct,used
R3794 T5043 T5040 arg2Of sites,lacking
R3795 T5043 T5041 arg1Of sites,NF-κB
R3796 T5043 T5042 arg1Of sites,binding
R3797 T5045 T5033 arg1Of used,As
R3798 T5045 T5036 arg1Of used,","
R3799 T5045 T5044 arg2Of used,was
R3800 T5046 T5047 arg2Of Cells,transfected
R3801 T5046 T5052 arg1Of Cells,did
R3802 T5046 T5054 arg1Of Cells,produce
R3803 T5047 T5048 arg1Of transfected,with
R3804 T5051 T5048 arg2Of construct,with
R3805 T5051 T5049 arg1Of construct,the
R3806 T5051 T5050 arg1Of construct,pTAL-SEAP
R3807 T5054 T5052 arg2Of produce,did
R3808 T5054 T5053 arg1Of produce,not
R3809 T5054 T5056 arg1Of produce,in
R3810 T5055 T5054 arg2Of SEAP,produce
R3811 T5058 T5059 arg1Of absence,or
R3812 T5059 T5056 arg2Of or,in
R3813 T5059 T5057 arg1Of or,the
R3814 T5059 T5061 arg1Of or,of
R3815 T5060 T5059 arg2Of presence,or
R3816 T5062 T5061 arg2Of M-CSF,of
R3817 T5062 T5063 arg1Of M-CSF,(
R3818 T5065 T5063 arg2Of 1B,(
R3819 T5065 T5064 arg1Of 1B,Figure
R3820 T5066 T5063 arg3Of ),(
R3821 T5067 T5069 arg1Of We,investigated
R3822 T5069 T5068 arg1Of investigated,next
R3823 T5071 T5072 arg1Of M-CSF,induced
R3824 T5072 T5069 arg2Of induced,investigated
R3825 T5072 T5070 arg1Of induced,whether
R3826 T5072 T5075 arg1Of induced,in
R3827 T5074 T5072 arg2Of activity,induced
R3828 T5074 T5073 arg1Of activity,NF-κB
R3829 T5080 T5075 arg2Of line,in
R3830 T5080 T5076 arg1Of line,the
R3831 T5080 T5077 arg1Of line,mouse
R3832 T5080 T5078 arg1Of line,macrophage
R3833 T5080 T5079 arg1Of line,cell
R3834 T5080 T5081 arg1Of line,","
R3835 T5082 T5081 arg2Of RAW,","
R3836 T5082 T5083 arg1Of RAW,264.7
R3837 T5086 T5084 arg1Of cells,RAW
R3838 T5086 T5085 arg1Of cells,264.7
R3839 T5086 T5087 arg1Of cells,were
R3840 T5086 T5088 arg2Of cells,transfected
R3841 T5088 T5087 arg2Of transfected,were
R3842 T5088 T5089 arg1Of transfected,with
R3843 T5093 T5092 arg1Of reporter,NF-κB-SEAP
R3844 T5093 T5094 arg1Of reporter,or
R3845 T5094 T5089 arg2Of or,with
R3846 T5094 T5090 arg1Of or,either
R3847 T5094 T5091 arg1Of or,the
R3848 T5097 T5094 arg2Of construct,or
R3849 T5097 T5095 arg1Of construct,control
R3850 T5097 T5096 arg1Of construct,pTAL-SEAP
R3851 T5099 T5098 arg2Of shown,As
R3852 T5099 T5100 arg1Of shown,in
R3853 T5102 T5100 arg2Of 1C,in
R3854 T5102 T5101 arg1Of 1C,Figure
R3855 T5105 T5104 arg1Of treatment,M-CSF
R3856 T5105 T5106 arg1Of treatment,of
R3857 T5105 T5110 arg1Of treatment,increased
R3858 T5109 T5106 arg2Of cells,of
R3859 T5109 T5107 arg1Of cells,RAW
R3860 T5109 T5108 arg1Of cells,264.7
R3861 T5110 T5098 arg1Of increased,As
R3862 T5110 T5103 arg1Of increased,","
R3863 T5110 T5114 arg1Of increased,by
R3864 T5110 T5116 arg1Of increased,over
R3865 T5113 T5110 arg2Of activity,increased
R3866 T5113 T5111 arg1Of activity,NF-κB
R3867 T5113 T5112 arg1Of activity,reporter
R3868 T5115 T5114 arg2Of 2.5-fold,by
R3869 T5117 T5116 arg2Of that,over
R3870 T5117 T5118 arg1Of that,of
R3871 T5120 T5118 arg2Of cells,of
R3872 T5120 T5119 arg1Of cells,non-treated
R3873 T5124 T5123 arg1Of data,our
R3874 T5124 T5125 arg1Of data,demonstrate
R3875 T5125 T5121 arg1Of demonstrate,Together
R3876 T5125 T5122 arg1Of demonstrate,","
R3877 T5127 T5128 arg1Of M-CSF,induced
R3878 T5128 T5125 arg2Of induced,demonstrate
R3879 T5128 T5126 arg1Of induced,that
R3880 T5131 T5128 arg2Of activity,induced
R3881 T5131 T5129 arg1Of activity,NF-κB
R3882 T5131 T5130 arg1Of activity,transcriptional
R3883 T5131 T5132 arg1Of activity,in
R3884 T5133 T5132 arg2Of macrophages,in
R17531 T22987 T22988 arg1Of M-CSF,induces
R17532 T22988 T22992 arg1Of induces,in
R17533 T22991 T22988 arg2Of activity,induces
R17534 T22991 T22989 arg1Of activity,NF-κB
R17535 T22991 T22990 arg1Of activity,transcriptional
R17536 T22993 T22992 arg2Of macrophages,in
R17537 T22995 T22994 arg2Of A,(
R17538 T22996 T22994 arg3Of ),(
R17539 T22997 T22994 arg1Of Nuclei,(
R17540 T22997 T22998 arg1Of Nuclei,were
R17541 T22997 T22999 arg2Of Nuclei,extracted
R17542 T22999 T22998 arg2Of extracted,were
R17543 T22999 T23000 arg1Of extracted,from
R17544 T23002 T23000 arg2Of MDMs,from
R17545 T23002 T23001 arg1Of MDMs,human
R17546 T23002 T23003 arg2Of MDMs,treated
R17547 T23003 T23004 arg1Of treated,without
R17548 T23003 T23006 arg1Of treated,with
R17549 T23004 T23005 arg1Of without,or
R17550 T23006 T23005 arg2Of with,or
R17551 T23007 T23004 arg2Of M-CSF,without
R17552 T23007 T23006 arg2Of M-CSF,with
R17553 T23007 T23008 arg1Of M-CSF,for
R17554 T23009 T23010 arg1Of 15,or
R17555 T23011 T23010 arg2Of 30,or
R17556 T23012 T23008 arg2Of minutes,for
R17557 T23012 T23009 arg1Of minutes,15
R17558 T23012 T23011 arg1Of minutes,30
R17559 T23013 T23022 arg1Of NF-κB,is
R17560 T23016 T23014 arg1Of activity,DNA
R17561 T23016 T23015 arg1Of activity,binding
R17562 T23016 T23017 arg1Of activity,was
R17563 T23016 T23018 arg2Of activity,analyzed
R17564 T23018 T23017 arg2Of analyzed,was
R17565 T23021 T23018 arg1Of Shown,analyzed
R17566 T23021 T23019 arg2Of Shown,by
R17567 T23021 T23020 arg1Of Shown,EMSA
R17568 T23022 T23017 modOf is,was
R17569 T23025 T23022 arg2Of blot,is
R17570 T23025 T23023 arg1Of blot,a
R17571 T23025 T23024 arg1Of blot,representative
R17572 T23025 T23026 arg1Of blot,from
R17573 T23029 T23026 arg2Of experiments,from
R17574 T23029 T23027 arg1Of experiments,three
R17575 T23029 T23028 arg1Of experiments,independent
R17576 T23030 T23031 arg1Of NS,:
R17577 T23030 T23045 modOf NS,were
R17578 T23034 T23033 arg2Of B,(
R17579 T23035 T23033 arg3Of ),(
R17580 T23037 T23032 arg1Of MDMs,non-stimulated.
R17581 T23037 T23033 arg1Of MDMs,(
R17582 T23037 T23036 arg1Of MDMs,Human
R17583 T23037 T23039 arg2Of MDMs,transfected
R17584 T23037 T23045 arg1Of MDMs,were
R17585 T23037 T23046 arg2Of MDMs,treated
R17586 T23037 T23053 arg2Of MDMs,incubated
R17587 T23039 T23038 arg1Of transfected,transiently
R17588 T23039 T23040 arg1Of transfected,with
R17589 T23041 T23042 arg1Of pTAL-SEAP,or
R17590 T23043 T23042 arg2Of pNF-κB-SEAP,or
R17591 T23044 T23040 arg2Of constructs,with
R17592 T23044 T23041 arg1Of constructs,pTAL-SEAP
R17593 T23044 T23043 arg1Of constructs,pNF-κB-SEAP
R17594 T23046 T23047 arg1Of treated,with
R17595 T23046 T23052 arg1Of treated,and
R17596 T23048 T23047 arg2Of M-CSF,with
R17597 T23048 T23049 arg1Of M-CSF,in
R17598 T23051 T23049 arg2Of medium,in
R17599 T23051 T23050 arg1Of medium,X-vivo
R17600 T23052 T23045 arg2Of and,were
R17601 T23053 T23052 arg2Of incubated,and
R17602 T23053 T23054 arg1Of incubated,for
R17603 T23053 T23057 arg1Of incubated,before
R17604 T23056 T23054 arg2Of hours,for
R17605 T23056 T23055 arg1Of hours,6
R17606 T23059 T23057 arg2Of collection,before
R17607 T23059 T23058 arg1Of collection,the
R17608 T23059 T23060 arg1Of collection,of
R17609 T23061 T23060 arg2Of medium,of
R17610 T23063 T23062 arg1Of activity,NF-κB
R17611 T23063 T23064 arg1Of activity,was
R17612 T23063 T23065 arg2Of activity,analyzed
R17613 T23065 T23064 arg2Of analyzed,was
R17614 T23065 T23066 arg1Of analyzed,by
R17615 T23065 T23076 arg1Of analyzed,and
R17616 T23067 T23066 arg2Of measuring,by
R17617 T23069 T23067 arg2Of amount,measuring
R17618 T23069 T23068 arg1Of amount,the
R17619 T23069 T23070 arg1Of amount,of
R17620 T23069 T23072 arg2Of amount,secreted
R17621 T23071 T23070 arg2Of SEAP,of
R17622 T23072 T23073 arg1Of secreted,into
R17623 T23075 T23073 arg2Of medium,into
R17624 T23075 T23074 arg1Of medium,the
R17625 T23077 T23078 arg1Of data,are
R17626 T23077 T23079 arg2Of data,expressed
R17627 T23079 T23076 arg2Of expressed,and
R17628 T23079 T23078 arg2Of expressed,are
R17629 T23079 T23080 arg1Of expressed,as
R17630 T23082 T23083 arg1Of increase,of
R17631 T23082 T23086 arg1Of increase,over
R17632 T23085 T23083 arg2Of activity,of
R17633 T23085 T23084 arg1Of activity,SEAP
R17634 T23087 T23086 arg2Of that,over
R17635 T23087 T23088 arg1Of that,in
R17636 T23092 T23088 arg2Of cells.,in
R17637 T23092 T23089 arg1Of cells.,pTAL-SEAP
R17638 T23092 T23090 arg2Of cells.,transfected
R17639 T23092 T23091 arg1Of cells.,resting
R17640 T23092 T23093 arg1Of cells.,(
R17641 T23094 T23093 arg2Of C,(
R17642 T23095 T23093 arg3Of ),(
R17643 T23098 T23081 arg1Of cells,fold
R17644 T23098 T23082 arg1Of cells,increase
R17645 T23098 T23096 arg1Of cells,RAW
R17646 T23098 T23097 arg1Of cells,264.7
R17647 T23098 T23099 arg1Of cells,were
R17648 T23098 T23101 arg2Of cells,transfected
R17649 T23101 T23080 arg2Of transfected,as
R17650 T23101 T23099 arg2Of transfected,were
R17651 T23101 T23100 arg1Of transfected,transiently
R17652 T23101 T23102 arg1Of transfected,with
R17653 T23103 T23104 arg1Of pTAL-SEAP,or
R17654 T23104 T23102 arg2Of or,with
R17655 T23106 T23104 arg2Of construct,or
R17656 T23106 T23105 arg1Of construct,pNF-κB-SEAP
R17657 T23107 T23108 arg1Of Cells,were
R17658 T23109 T23108 arg2Of serum,were
R17659 T23109 T23110 arg2Of serum,starved
R17660 T23110 T23111 arg1Of starved,for
R17661 T23112 T23115 arg1Of 4,to
R17662 T23114 T23113 arg1Of prior,hours
R17663 T23115 T23114 arg1Of to,prior
R17664 T23117 T23115 arg2Of hours,to
R17665 T23117 T23116 arg1Of hours,2
R17666 T23118 T23111 arg2Of stimulation,for
R17667 T23118 T23112 arg1Of stimulation,4
R17668 T23118 T23119 arg1Of stimulation,with
R17669 T23122 T23119 arg2Of M-CSF,with
R17670 T23122 T23120 arg1Of M-CSF,mouse
R17671 T23122 T23121 arg1Of M-CSF,recombinant
R17672 T23122 T23123 arg1Of M-CSF,(
R17673 T23125 T23123 arg2Of ng/ml,(
R17674 T23125 T23124 arg1Of ng/ml,100
R17675 T23126 T23123 arg3Of ),(
R17676 T23128 T23127 arg1Of media,Culture
R17677 T23128 T23129 arg1Of media,was
R17678 T23128 T23130 arg2Of media,collected
R17679 T23130 T23129 arg2Of collected,was
R17680 T23130 T23131 modOf collected,to
R17681 T23132 T23131 arg1Of measure,to
R17682 T23134 T23132 arg2Of production,measure
R17683 T23134 T23133 arg1Of production,SEAP
R17684 T23135 T23136 arg1Of Data,are
R17685 T23135 T23137 arg2Of Data,expressed
R17686 T23137 T23136 arg2Of expressed,are
R17687 T23137 T23140 arg1Of expressed,S.E.M
R17688 T23137 T23141 arg1Of expressed,","
R17689 T23137 T23142 arg1Of expressed,for
R17690 T23140 T23138 arg1Of S.E.M,mean
R17691 T23140 T23139 arg1Of S.E.M,±
R17692 T23145 T23142 arg2Of experiments,for
R17693 T23145 T23143 arg1Of experiments,three
R17694 T23145 T23144 arg1Of experiments,independent

sentences

Id Subject Object Predicate Lexical cue
T4664 0-131 Sentence denotes M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7
T4665 132-386 Sentence denotes To determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A).
T4666 387-455 Sentence denotes Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA.
T4667 456-763 Sentence denotes In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B).
T4668 764-839 Sentence denotes As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used.
T4669 840-956 Sentence denotes Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).
T4670 1987-2090 Sentence denotes We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7.
T4671 2091-2191 Sentence denotes RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct.
T4672 2192-2327 Sentence denotes As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells.
T4673 2328-2424 Sentence denotes Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages.
T22810 1001-1061 Sentence denotes M-CSF induces NF-κB transcriptional activity in macrophages.
T22811 1062-1155 Sentence denotes (A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes.
T22812 1156-1270 Sentence denotes NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments.
T22813 1271-1470 Sentence denotes NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium.
T22814 1471-1752 Sentence denotes NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct.
T22815 1753-1860 Sentence denotes Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml).
T22816 1861-1916 Sentence denotes Culture media was collected to measure SEAP production.
T22817 1917-1984 Sentence denotes Data are expressed mean ± S.E.M, for three independent experiments.
T62 0-131 Sentence denotes M-CSF Induces NF-κB Transcriptional Activity in Human Monocyte–Derived Macrophages (MDMs) and Mouse Macrophage Cell Line, RAW 264.7
T63 132-386 Sentence denotes To determine if M-CSF induced NF-κB DNA binding in human macrophages, we performed EMSA analysis on nuclear lysates from M-CSF-treated MDMs. Similar to previous reports [11], nuclear NF-κB constitutively bound DNA in non-stimulated monocytes (Figure 1A).
T64 387-455 Sentence denotes Interestingly, adding M-CSF did not alter NF-κB DNA binding by EMSA.
T65 456-763 Sentence denotes In contrast, after transiently transfecting human MDMs with pNF-κB-SEAP constructs containing four NF-κB consensus binding sequences, M-CSF treatment of the transfected cells resulted in a 2.3-fold increase in SEAP release in the culture media compared to PBS (vehicle)-treated transfected MDMs (Figure 1B).
T66 764-839 Sentence denotes As a control, the pTAL-SEAP construct lacking NF-κB binding sites was used.
T67 840-956 Sentence denotes Cells transfected with the pTAL-SEAP construct did not produce SEAP in the absence or presence of M-CSF (Figure 1B).
T68 957-1061 Sentence denotes 10.1371/journal.pone.0028081.g001 Figure 1 M-CSF induces NF-κB transcriptional activity in macrophages.
T69 1062-1155 Sentence denotes (A) Nuclei were extracted from human MDMs treated without or with M-CSF for 15 or 30 minutes.
T70 1156-1270 Sentence denotes NF-κB DNA binding activity was analyzed by EMSA Shown is a representative blot from three independent experiments.
T71 1271-1470 Sentence denotes NS: non-stimulated. (B) Human MDMs transiently transfected with pTAL-SEAP or pNF-κB-SEAP constructs were treated with M-CSF in X-vivo medium and incubated for 6 hours before the collection of medium.
T72 1471-1752 Sentence denotes NF-κB activity was analyzed by measuring the amount of SEAP secreted into the medium and data are expressed as fold increase of SEAP activity over that in pTAL-SEAP transfected resting cells. (C) RAW 264.7 cells were transiently transfected with pTAL-SEAP or pNF-κB-SEAP construct.
T73 1753-1860 Sentence denotes Cells were serum starved for 4 hours prior to 2 hours stimulation with mouse recombinant M-CSF (100 ng/ml).
T74 1861-1916 Sentence denotes Culture media was collected to measure SEAP production.
T75 1917-1984 Sentence denotes Data are expressed mean ± S.E.M, for three independent experiments.
T76 1987-2090 Sentence denotes We next investigated whether M-CSF induced NF-κB activity in the mouse macrophage cell line, RAW 264.7.
T77 2091-2191 Sentence denotes RAW 264.7 cells were transfected with either the NF-κB-SEAP reporter or control pTAL-SEAP construct.
T78 2192-2327 Sentence denotes As shown in Figure 1C, M-CSF treatment of RAW 264.7 cells increased NF-κB reporter activity by 2.5-fold over that of non-treated cells.
T79 2328-2424 Sentence denotes Together, our data demonstrate that M-CSF induced NF-κB transcriptional activity in macrophages.

2_test

Id Subject Object Predicate Lexical cue
22216091-11073986-90609709 302-304 11073986 denotes 11

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4674 0-5 NNP denotes M-CSF
T4675 6-13 VBZ denotes Induces
T4676 14-19 NNP denotes NF-κB
T4677 20-35 NNP denotes Transcriptional
T4678 36-44 NNP denotes Activity
T4679 45-47 IN denotes in
T4680 48-53 NNP denotes Human
T4681 54-62 NNP denotes Monocyte
T4682 63-70 NNP denotes Derived
T4683 71-82 NNP denotes Macrophages
T4684 83-84 -LRB- denotes (
T4685 84-88 NNP denotes MDMs
T4686 88-89 -RRB- denotes )
T4687 90-93 CC denotes and
T4688 94-99 NNP denotes Mouse
T4689 100-110 NNP denotes Macrophage
T4690 111-115 NNP denotes Cell
T4691 116-120 NNP denotes Line
T4692 120-121 , denotes ,
T4693 122-125 NNP denotes RAW
T4694 126-131 CD denotes 264.7
T4695 132-134 TO denotes To
T4696 135-144 VB denotes determine
T4697 145-147 IN denotes if
T4698 148-153 NNP denotes M-CSF
T4699 154-161 VBD denotes induced
T4700 162-167 NNP denotes NF-κB
T4701 168-171 NNP denotes DNA
T4702 172-179 JJ denotes binding
T4703 180-182 IN denotes in
T4704 183-188 JJ denotes human
T4705 189-200 NNS denotes macrophages
T4706 200-201 , denotes ,
T4707 202-204 PRP denotes we
T4708 205-214 VBD denotes performed
T4709 215-219 NNP denotes EMSA
T4710 220-228 NN denotes analysis
T4711 229-231 IN denotes on
T4712 232-239 JJ denotes nuclear
T4713 240-247 NNS denotes lysates
T4714 248-252 IN denotes from
T4715 253-266 JJ denotes M-CSF-treated
T4716 267-271 NNS denotes MDMs
T4717 271-272 . denotes .
T4718 273-280 JJ denotes Similar
T4719 281-283 TO denotes to
T4720 284-292 JJ denotes previous
T4721 293-300 NNS denotes reports
T4722 301-302 NNP denotes [
T4723 302-304 CD denotes 11
T4724 304-305 NNP denotes ]
T4725 305-306 , denotes ,
T4726 307-314 JJ denotes nuclear
T4727 315-320 NN denotes NF-κB
T4728 321-335 RB denotes constitutively
T4729 336-341 VBN denotes bound
T4730 342-345 NN denotes DNA
T4731 346-348 IN denotes in
T4732 349-363 JJ denotes non-stimulated
T4733 364-373 NNS denotes monocytes
T4734 374-375 -LRB- denotes (
T4735 375-381 NN denotes Figure
T4736 382-384 CD denotes 1A
T4737 384-385 -RRB- denotes )
T4738 385-386 . denotes .
T4739 387-400 RB denotes Interestingly
T4740 400-401 , denotes ,
T4741 402-408 VBG denotes adding
T4742 409-414 NNP denotes M-CSF
T4743 415-418 VBD denotes did
T4744 419-422 RB denotes not
T4745 423-428 VB denotes alter
T4746 429-434 NNP denotes NF-κB
T4747 435-438 NNP denotes DNA
T4748 439-446 JJ denotes binding
T4749 447-449 IN denotes by
T4750 450-454 NNP denotes EMSA
T4751 454-455 . denotes .
T4752 456-458 IN denotes In
T4753 459-467 NN denotes contrast
T4754 467-468 , denotes ,
T4755 469-474 IN denotes after
T4756 475-486 RB denotes transiently
T4757 487-499 VBG denotes transfecting
T4758 500-505 JJ denotes human
T4759 506-510 NNS denotes MDMs
T4760 511-515 IN denotes with
T4761 516-527 JJ denotes pNF-κB-SEAP
T4762 528-538 NNS denotes constructs
T4763 539-549 VBG denotes containing
T4764 550-554 CD denotes four
T4765 555-560 JJ denotes NF-κB
T4766 561-570 NN denotes consensus
T4767 571-578 JJ denotes binding
T4768 579-588 NNS denotes sequences
T4769 588-589 , denotes ,
T4770 590-595 NNP denotes M-CSF
T4771 596-605 NN denotes treatment
T4772 606-608 IN denotes of
T4773 609-612 DT denotes the
T4774 613-624 VBN denotes transfected
T4775 625-630 NNS denotes cells
T4776 631-639 VBD denotes resulted
T4777 640-642 IN denotes in
T4778 643-644 DT denotes a
T4779 645-653 JJ denotes 2.3-fold
T4780 654-662 NN denotes increase
T4781 663-665 IN denotes in
T4782 666-670 NNP denotes SEAP
T4783 671-678 NN denotes release
T4784 679-681 IN denotes in
T4785 682-685 DT denotes the
T4786 686-693 NN denotes culture
T4787 694-699 NNS denotes media
T4788 700-708 VBN denotes compared
T4789 709-711 TO denotes to
T4790 712-715 NNP denotes PBS
T4791 716-717 -LRB- denotes (
T4792 717-724 NN denotes vehicle
T4793 724-725 -RRB- denotes )
T4794 725-726 : denotes -
T4795 726-733 VBN denotes treated
T4796 734-745 VBN denotes transfected
T4797 746-750 NNS denotes MDMs
T4798 751-752 -LRB- denotes (
T4799 752-758 NN denotes Figure
T4800 759-761 NN denotes 1B
T4801 761-762 -RRB- denotes )
T4802 762-763 . denotes .
T4803 764-766 IN denotes As
T4804 767-768 DT denotes a
T4805 769-776 NN denotes control
T4806 776-777 , denotes ,
T4807 778-781 DT denotes the
T4808 782-791 NNP denotes pTAL-SEAP
T4809 792-801 VB denotes construct
T4810 802-809 VBG denotes lacking
T4811 810-815 JJ denotes NF-κB
T4812 816-823 JJ denotes binding
T4813 824-829 NNS denotes sites
T4814 830-833 VBD denotes was
T4815 834-838 VBN denotes used
T4816 838-839 . denotes .
T4817 840-845 NNS denotes Cells
T4818 846-857 VBN denotes transfected
T4819 858-862 IN denotes with
T4820 863-866 DT denotes the
T4821 867-876 NNP denotes pTAL-SEAP
T4822 877-886 VBP denotes construct
T4823 887-890 VBD denotes did
T4824 891-894 RB denotes not
T4825 895-902 VB denotes produce
T4826 903-907 NNP denotes SEAP
T4827 908-910 IN denotes in
T4828 911-914 DT denotes the
T4829 915-922 NN denotes absence
T4830 923-925 CC denotes or
T4831 926-934 NN denotes presence
T4832 935-937 IN denotes of
T4833 938-943 NNP denotes M-CSF
T4834 944-945 -LRB- denotes (
T4835 945-951 NNP denotes Figure
T4836 952-954 NNP denotes 1B
T4837 954-955 -RRB- denotes )
T4838 955-956 . denotes .
T4839 1987-1989 PRP denotes We
T4840 1990-1994 JJ denotes next
T4841 1995-2007 VBD denotes investigated
T4842 2008-2015 IN denotes whether
T4843 2016-2021 NNP denotes M-CSF
T4844 2022-2029 VBD denotes induced
T4845 2030-2035 JJ denotes NF-κB
T4846 2036-2044 NN denotes activity
T4847 2045-2047 IN denotes in
T4848 2048-2051 DT denotes the
T4849 2052-2057 NN denotes mouse
T4850 2058-2068 NN denotes macrophage
T4851 2069-2073 NN denotes cell
T4852 2074-2078 NN denotes line
T4853 2078-2079 , denotes ,
T4854 2080-2083 NNP denotes RAW
T4855 2084-2089 CD denotes 264.7
T4856 2089-2090 . denotes .
T4857 2091-2094 NNP denotes RAW
T4858 2095-2100 CD denotes 264.7
T4859 2101-2106 NNS denotes cells
T4860 2107-2111 VBD denotes were
T4861 2112-2123 VBN denotes transfected
T4862 2124-2128 IN denotes with
T4863 2129-2135 CC denotes either
T4864 2136-2139 DT denotes the
T4865 2140-2150 NNP denotes NF-κB-SEAP
T4866 2151-2159 NN denotes reporter
T4867 2160-2162 CC denotes or
T4868 2163-2170 NN denotes control
T4869 2171-2180 JJ denotes pTAL-SEAP
T4870 2181-2190 VBP denotes construct
T4871 2190-2191 . denotes .
T4872 2192-2194 IN denotes As
T4873 2195-2200 VBN denotes shown
T4874 2201-2203 IN denotes in
T4875 2204-2210 NN denotes Figure
T4876 2211-2213 CD denotes 1C
T4877 2213-2214 , denotes ,
T4878 2215-2220 NNP denotes M-CSF
T4879 2221-2230 NN denotes treatment
T4880 2231-2233 IN denotes of
T4881 2234-2237 NNP denotes RAW
T4882 2238-2243 CD denotes 264.7
T4883 2244-2249 NNS denotes cells
T4884 2250-2259 VBD denotes increased
T4885 2260-2265 JJ denotes NF-κB
T4886 2266-2274 NN denotes reporter
T4887 2275-2283 NN denotes activity
T4888 2284-2286 IN denotes by
T4889 2287-2295 CD denotes 2.5-fold
T4890 2296-2300 IN denotes over
T4891 2301-2305 DT denotes that
T4892 2306-2308 IN denotes of
T4893 2309-2320 JJ denotes non-treated
T4894 2321-2326 NNS denotes cells
T4895 2326-2327 . denotes .
T4896 2328-2336 RB denotes Together
T4897 2336-2337 , denotes ,
T4898 2338-2341 PRP$ denotes our
T4899 2342-2346 NNS denotes data
T4900 2347-2358 VBP denotes demonstrate
T4901 2359-2363 IN denotes that
T4902 2364-2369 NNP denotes M-CSF
T4903 2370-2377 VBD denotes induced
T4904 2378-2383 NNP denotes NF-κB
T4905 2384-2399 JJ denotes transcriptional
T4906 2400-2408 NN denotes activity
T4907 2409-2411 IN denotes in
T4908 2412-2423 NNS denotes macrophages
T4909 2423-2424 . denotes .
T22818 1001-1006 NNP denotes M-CSF
T22819 1007-1014 VBZ denotes induces
T22820 1015-1020 JJ denotes NF-κB
T22821 1021-1036 JJ denotes transcriptional
T22822 1037-1045 NN denotes activity
T22823 1046-1048 IN denotes in
T22824 1049-1060 NNS denotes macrophages
T22825 1060-1061 . denotes .
T22826 1062-1063 -LRB- denotes (
T22827 1063-1064 NNP denotes A
T22828 1064-1065 -RRB- denotes )
T22829 1066-1072 NNP denotes Nuclei
T22830 1073-1077 VBD denotes were
T22831 1078-1087 VBN denotes extracted
T22832 1088-1092 IN denotes from
T22833 1093-1098 JJ denotes human
T22834 1099-1103 NNS denotes MDMs
T22835 1104-1111 VBN denotes treated
T22836 1112-1119 IN denotes without
T22837 1120-1122 CC denotes or
T22838 1123-1127 IN denotes with
T22839 1128-1133 NNP denotes M-CSF
T22840 1134-1137 IN denotes for
T22841 1138-1140 CD denotes 15
T22842 1141-1143 CC denotes or
T22843 1144-1146 CD denotes 30
T22844 1147-1154 NNS denotes minutes
T22845 1154-1155 . denotes .
T22846 1156-1161 NNP denotes NF-κB
T22847 1162-1165 NNP denotes DNA
T22848 1166-1173 JJ denotes binding
T22849 1174-1182 NN denotes activity
T22850 1183-1186 VBD denotes was
T22851 1187-1195 VBN denotes analyzed
T22852 1196-1198 IN denotes by
T22853 1199-1203 NNP denotes EMSA
T22854 1204-1209 NNP denotes Shown
T22855 1210-1212 VBZ denotes is
T22856 1213-1214 DT denotes a
T22857 1215-1229 JJ denotes representative
T22858 1230-1234 NN denotes blot
T22859 1235-1239 IN denotes from
T22860 1240-1245 CD denotes three
T22861 1246-1257 JJ denotes independent
T22862 1258-1269 NNS denotes experiments
T22863 1269-1270 . denotes .
T22864 1271-1273 NNP denotes NS
T22865 1273-1274 : denotes :
T22866 1275-1289 JJ denotes non-stimulated
T22867 1289-1290 . denotes .
T22868 1291-1292 -LRB- denotes (
T22869 1292-1293 NNP denotes B
T22870 1293-1294 -RRB- denotes )
T22871 1295-1300 NNP denotes Human
T22872 1301-1305 NNP denotes MDMs
T22873 1306-1317 RB denotes transiently
T22874 1318-1329 VBD denotes transfected
T22875 1330-1334 IN denotes with
T22876 1335-1344 JJ denotes pTAL-SEAP
T22877 1345-1347 CC denotes or
T22878 1348-1359 JJ denotes pNF-κB-SEAP
T22879 1360-1370 NNS denotes constructs
T22880 1371-1375 VBD denotes were
T22881 1376-1383 VBN denotes treated
T22882 1384-1388 IN denotes with
T22883 1389-1394 NNP denotes M-CSF
T22884 1395-1397 IN denotes in
T22885 1398-1404 NNP denotes X-vivo
T22886 1405-1411 NN denotes medium
T22887 1412-1415 CC denotes and
T22888 1416-1425 VBN denotes incubated
T22889 1426-1429 IN denotes for
T22890 1430-1431 CD denotes 6
T22891 1432-1437 NNS denotes hours
T22892 1438-1444 IN denotes before
T22893 1445-1448 DT denotes the
T22894 1449-1459 NN denotes collection
T22895 1460-1462 IN denotes of
T22896 1463-1469 NN denotes medium
T22897 1469-1470 . denotes .
T22898 1471-1476 JJ denotes NF-κB
T22899 1477-1485 NN denotes activity
T22900 1486-1489 VBD denotes was
T22901 1490-1498 VBN denotes analyzed
T22902 1499-1501 IN denotes by
T22903 1502-1511 VBG denotes measuring
T22904 1512-1515 DT denotes the
T22905 1516-1522 NN denotes amount
T22906 1523-1525 IN denotes of
T22907 1526-1530 NNP denotes SEAP
T22908 1531-1539 VBD denotes secreted
T22909 1540-1544 IN denotes into
T22910 1545-1548 DT denotes the
T22911 1549-1555 NN denotes medium
T22912 1556-1559 CC denotes and
T22913 1560-1564 NNS denotes data
T22914 1565-1568 VBP denotes are
T22915 1569-1578 VBN denotes expressed
T22916 1579-1581 IN denotes as
T22917 1582-1586 VBP denotes fold
T22918 1587-1595 NN denotes increase
T22919 1596-1598 IN denotes of
T22920 1599-1603 NNP denotes SEAP
T22921 1604-1612 NN denotes activity
T22922 1613-1617 IN denotes over
T22923 1618-1622 DT denotes that
T22924 1623-1625 IN denotes in
T22925 1626-1635 JJ denotes pTAL-SEAP
T22926 1636-1647 VBN denotes transfected
T22927 1648-1655 VBG denotes resting
T22928 1656-1661 NNS denotes cells
T22929 1661-1662 . denotes .
T22930 1663-1664 -LRB- denotes (
T22931 1664-1665 NNP denotes C
T22932 1665-1666 -RRB- denotes )
T22933 1667-1670 NNP denotes RAW
T22934 1671-1676 CD denotes 264.7
T22935 1677-1682 NNS denotes cells
T22936 1683-1687 VBD denotes were
T22937 1688-1699 RB denotes transiently
T22938 1700-1711 VBN denotes transfected
T22939 1712-1716 IN denotes with
T22940 1717-1726 NNP denotes pTAL-SEAP
T22941 1727-1729 CC denotes or
T22942 1730-1741 NNP denotes pNF-κB-SEAP
T22943 1742-1751 VBP denotes construct
T22944 1751-1752 . denotes .
T22945 1753-1758 NNS denotes Cells
T22946 1759-1763 VBD denotes were
T22947 1764-1769 JJ denotes serum
T22948 1770-1777 VBN denotes starved
T22949 1778-1781 IN denotes for
T22950 1782-1783 CD denotes 4
T22951 1784-1789 NNS denotes hours
T22952 1790-1795 RB denotes prior
T22953 1796-1798 TO denotes to
T22954 1799-1800 CD denotes 2
T22955 1801-1806 NNS denotes hours
T22956 1807-1818 NN denotes stimulation
T22957 1819-1823 IN denotes with
T22958 1824-1829 NN denotes mouse
T22959 1830-1841 JJ denotes recombinant
T22960 1842-1847 NNP denotes M-CSF
T22961 1848-1849 -LRB- denotes (
T22962 1849-1852 CD denotes 100
T22963 1853-1858 NN denotes ng/ml
T22964 1858-1859 -RRB- denotes )
T22965 1859-1860 . denotes .
T22966 1861-1868 NNP denotes Culture
T22967 1869-1874 NNS denotes media
T22968 1875-1878 VBD denotes was
T22969 1879-1888 VBN denotes collected
T22970 1889-1891 TO denotes to
T22971 1892-1899 VB denotes measure
T22972 1900-1904 NNP denotes SEAP
T22973 1905-1915 NN denotes production
T22974 1915-1916 . denotes .
T22975 1917-1921 NNS denotes Data
T22976 1922-1925 VBP denotes are
T22977 1926-1935 VBN denotes expressed
T22978 1936-1940 JJ denotes mean
T22979 1941-1942 NN denotes ±
T22980 1943-1948 NNP denotes S.E.M
T22981 1948-1949 , denotes ,
T22982 1950-1953 IN denotes for
T22983 1954-1959 CD denotes three
T22984 1960-1971 JJ denotes independent
T22985 1972-1983 NNS denotes experiments
T22986 1983-1984 . denotes .
R3429 T4674 T4675 nsubj M-CSF,Induces
R3430 T4675 T4675 ROOT Induces,Induces
R3431 T4676 T4678 compound NF-κB,Activity
R3432 T4677 T4678 compound Transcriptional,Activity
R3433 T4678 T4675 dobj Activity,Induces
R3434 T4679 T4678 prep in,Activity
R3435 T4680 T4681 compound Human,Monocyte
R3436 T4681 T4679 pobj Monocyte,in
R3437 T4682 T4683 compound Derived,Macrophages
R3438 T4683 T4678 conj Macrophages,Activity
R3439 T4684 T4685 punct (,MDMs
R3440 T4685 T4683 appos MDMs,Macrophages
R3441 T4686 T4685 punct ),MDMs
R3442 T4687 T4683 cc and,Macrophages
R3443 T4688 T4691 compound Mouse,Line
R3444 T4689 T4691 compound Macrophage,Line
R3445 T4690 T4691 compound Cell,Line
R3446 T4691 T4683 conj Line,Macrophages
R3447 T4692 T4691 punct ",",Line
R3448 T4693 T4691 conj RAW,Line
R3449 T4694 T4693 nummod 264.7,RAW
R3450 T4695 T4696 aux To,determine
R3451 T4696 T4675 advcl determine,Induces
R3452 T4697 T4699 mark if,induced
R3453 T4698 T4699 nsubj M-CSF,induced
R3454 T4699 T4708 advcl induced,performed
R3455 T4700 T4701 compound NF-κB,DNA
R3456 T4701 T4699 dobj DNA,induced
R3457 T4702 T4699 advcl binding,induced
R3458 T4703 T4702 prep in,binding
R3459 T4704 T4705 amod human,macrophages
R3460 T4705 T4703 pobj macrophages,in
R3461 T4706 T4708 punct ",",performed
R3462 T4707 T4708 nsubj we,performed
R3463 T4708 T4675 conj performed,Induces
R3464 T4709 T4710 compound EMSA,analysis
R3465 T4710 T4708 dobj analysis,performed
R3466 T4711 T4708 prep on,performed
R3467 T4712 T4713 amod nuclear,lysates
R3468 T4713 T4711 pobj lysates,on
R3469 T4714 T4713 prep from,lysates
R3470 T4715 T4716 amod M-CSF-treated,MDMs
R3471 T4716 T4714 pobj MDMs,from
R3472 T4717 T4708 punct .,performed
R3473 T4718 T4718 ROOT Similar,Similar
R3474 T4719 T4718 prep to,Similar
R3475 T4720 T4721 amod previous,reports
R3476 T4721 T4719 pobj reports,to
R3477 T4722 T4724 compound [,]
R3478 T4723 T4724 compound 11,]
R3479 T4724 T4718 appos ],Similar
R3480 T4725 T4724 punct ",",]
R3481 T4726 T4727 amod nuclear,NF-κB
R3482 T4727 T4724 appos NF-κB,]
R3483 T4728 T4729 advmod constitutively,bound
R3484 T4729 T4730 amod bound,DNA
R3485 T4730 T4724 conj DNA,]
R3486 T4731 T4730 prep in,DNA
R3487 T4732 T4733 amod non-stimulated,monocytes
R3488 T4733 T4731 pobj monocytes,in
R3489 T4734 T4735 punct (,Figure
R3490 T4735 T4733 parataxis Figure,monocytes
R3491 T4736 T4735 nummod 1A,Figure
R3492 T4737 T4735 punct ),Figure
R3493 T4738 T4718 punct .,Similar
R3494 T4739 T4745 advmod Interestingly,alter
R3495 T4740 T4745 punct ",",alter
R3496 T4741 T4745 advcl adding,alter
R3497 T4742 T4741 dobj M-CSF,adding
R3498 T4743 T4745 aux did,alter
R3499 T4744 T4745 neg not,alter
R3500 T4745 T4745 ROOT alter,alter
R3501 T4746 T4747 compound NF-κB,DNA
R3502 T4747 T4745 dobj DNA,alter
R3503 T4748 T4747 acl binding,DNA
R3504 T4749 T4748 agent by,binding
R3505 T4750 T4749 pobj EMSA,by
R3506 T4751 T4745 punct .,alter
R3507 T4752 T4776 prep In,resulted
R3508 T4753 T4752 pobj contrast,In
R3509 T4754 T4776 punct ",",resulted
R3510 T4755 T4776 prep after,resulted
R3511 T4756 T4757 advmod transiently,transfecting
R3512 T4757 T4755 pcomp transfecting,after
R3513 T4758 T4759 amod human,MDMs
R3514 T4759 T4757 dobj MDMs,transfecting
R3515 T4760 T4757 prep with,transfecting
R3516 T4761 T4762 compound pNF-κB-SEAP,constructs
R3517 T4762 T4760 pobj constructs,with
R3518 T4763 T4762 acl containing,constructs
R3519 T4764 T4768 nummod four,sequences
R3520 T4765 T4768 amod NF-κB,sequences
R3521 T4766 T4768 amod consensus,sequences
R3522 T4767 T4768 amod binding,sequences
R3523 T4768 T4763 dobj sequences,containing
R3524 T4769 T4768 punct ",",sequences
R3525 T4770 T4771 compound M-CSF,treatment
R3526 T4771 T4776 nsubj treatment,resulted
R3527 T4772 T4771 prep of,treatment
R3528 T4773 T4775 det the,cells
R3529 T4774 T4775 amod transfected,cells
R3530 T4775 T4772 pobj cells,of
R3531 T4776 T4776 ROOT resulted,resulted
R3532 T4777 T4776 prep in,resulted
R3533 T4778 T4780 det a,increase
R3534 T4779 T4780 amod 2.3-fold,increase
R3535 T4780 T4777 pobj increase,in
R3536 T4781 T4780 prep in,increase
R3537 T4782 T4783 compound SEAP,release
R3538 T4783 T4781 pobj release,in
R3539 T4784 T4783 prep in,release
R3540 T4785 T4787 det the,media
R3541 T4786 T4787 compound culture,media
R3542 T4787 T4784 pobj media,in
R3543 T4788 T4776 prep compared,resulted
R3544 T4789 T4788 prep to,compared
R3545 T4790 T4789 pobj PBS,to
R3546 T4791 T4792 punct (,vehicle
R3547 T4792 T4790 appos vehicle,PBS
R3548 T4793 T4790 punct ),PBS
R3549 T4794 T4795 punct -,treated
R3550 T4795 T4797 amod treated,MDMs
R3551 T4796 T4797 amod transfected,MDMs
R3552 T4797 T4789 pobj MDMs,to
R3553 T4798 T4800 punct (,1B
R3554 T4799 T4800 compound Figure,1B
R3555 T4800 T4797 appos 1B,MDMs
R3556 T4801 T4800 punct ),1B
R3557 T4802 T4776 punct .,resulted
R3558 T4803 T4809 prep As,construct
R3559 T4804 T4805 det a,control
R3560 T4805 T4803 pobj control,As
R3561 T4806 T4809 punct ",",construct
R3562 T4807 T4808 det the,pTAL-SEAP
R3563 T4808 T4809 nsubj pTAL-SEAP,construct
R3564 T4809 T4809 ROOT construct,construct
R3565 T4810 T4809 xcomp lacking,construct
R3566 T4811 T4813 amod NF-κB,sites
R3567 T4812 T4813 amod binding,sites
R3568 T4813 T4815 nsubjpass sites,used
R3569 T4814 T4815 auxpass was,used
R3570 T4815 T4810 ccomp used,lacking
R3571 T4816 T4809 punct .,construct
R3572 T4817 T4822 nsubj Cells,construct
R3573 T4818 T4817 acl transfected,Cells
R3574 T4819 T4818 prep with,transfected
R3575 T4820 T4821 det the,pTAL-SEAP
R3576 T4821 T4819 pobj pTAL-SEAP,with
R3577 T4822 T4822 ROOT construct,construct
R3578 T4823 T4825 aux did,produce
R3579 T4824 T4825 neg not,produce
R3580 T4825 T4822 conj produce,construct
R3581 T4826 T4825 dobj SEAP,produce
R3582 T4827 T4825 prep in,produce
R3583 T4828 T4829 det the,absence
R3584 T4829 T4827 pobj absence,in
R3585 T4830 T4829 cc or,absence
R3586 T4831 T4829 conj presence,absence
R3587 T4832 T4829 prep of,absence
R3588 T4833 T4832 pobj M-CSF,of
R3589 T4834 T4836 punct (,1B
R3590 T4835 T4836 compound Figure,1B
R3591 T4836 T4833 appos 1B,M-CSF
R3592 T4837 T4833 punct ),M-CSF
R3593 T4838 T4822 punct .,construct
R3594 T4839 T4841 nsubj We,investigated
R3595 T4840 T4841 advmod next,investigated
R3596 T4841 T4841 ROOT investigated,investigated
R3597 T4842 T4844 mark whether,induced
R3598 T4843 T4844 nsubj M-CSF,induced
R3599 T4844 T4841 ccomp induced,investigated
R3600 T4845 T4846 compound NF-κB,activity
R3601 T4846 T4844 dobj activity,induced
R3602 T4847 T4844 prep in,induced
R3603 T4848 T4852 det the,line
R3604 T4849 T4852 compound mouse,line
R3605 T4850 T4852 compound macrophage,line
R3606 T4851 T4852 compound cell,line
R3607 T4852 T4847 pobj line,in
R3608 T4853 T4852 punct ",",line
R3609 T4854 T4852 appos RAW,line
R3610 T4855 T4854 nummod 264.7,RAW
R3611 T4856 T4841 punct .,investigated
R3612 T4857 T4861 nsubjpass RAW,transfected
R3613 T4858 T4859 nummod 264.7,cells
R3614 T4859 T4861 nsubjpass cells,transfected
R3615 T4860 T4861 auxpass were,transfected
R3616 T4861 T4861 ROOT transfected,transfected
R3617 T4862 T4861 prep with,transfected
R3618 T4863 T4866 preconj either,reporter
R3619 T4864 T4866 det the,reporter
R3620 T4865 T4866 compound NF-κB-SEAP,reporter
R3621 T4866 T4870 nsubj reporter,construct
R3622 T4867 T4866 cc or,reporter
R3623 T4868 T4866 conj control,reporter
R3624 T4869 T4870 amod pTAL-SEAP,construct
R3625 T4870 T4870 ROOT construct,construct
R3626 T4871 T4870 punct .,construct
R3627 T4872 T4873 mark As,shown
R3628 T4873 T4884 advcl shown,increased
R3629 T4874 T4873 prep in,shown
R3630 T4875 T4874 pobj Figure,in
R3631 T4876 T4875 nummod 1C,Figure
R3632 T4877 T4875 punct ",",Figure
R3633 T4878 T4879 compound M-CSF,treatment
R3634 T4879 T4875 appos treatment,Figure
R3635 T4880 T4879 prep of,treatment
R3636 T4881 T4880 pobj RAW,of
R3637 T4882 T4883 nummod 264.7,cells
R3638 T4883 T4884 nsubj cells,increased
R3639 T4884 T4884 ROOT increased,increased
R3640 T4885 T4887 amod NF-κB,activity
R3641 T4886 T4887 compound reporter,activity
R3642 T4887 T4884 dobj activity,increased
R3643 T4888 T4884 prep by,increased
R3644 T4889 T4888 pobj 2.5-fold,by
R3645 T4890 T4884 prep over,increased
R3646 T4891 T4890 pobj that,over
R3647 T4892 T4891 prep of,that
R3648 T4893 T4894 amod non-treated,cells
R3649 T4894 T4892 pobj cells,of
R3650 T4895 T4884 punct .,increased
R3651 T4896 T4900 advmod Together,demonstrate
R3652 T4897 T4900 punct ",",demonstrate
R3653 T4898 T4899 poss our,data
R3654 T4899 T4900 nsubj data,demonstrate
R3655 T4900 T4900 ROOT demonstrate,demonstrate
R3656 T4901 T4903 mark that,induced
R3657 T4902 T4903 nsubj M-CSF,induced
R3658 T4903 T4900 ccomp induced,demonstrate
R3659 T4904 T4906 compound NF-κB,activity
R3660 T4905 T4906 compound transcriptional,activity
R3661 T4906 T4903 dobj activity,induced
R3662 T4907 T4906 prep in,activity
R3663 T4908 T4907 pobj macrophages,in
R3664 T4909 T4900 punct .,demonstrate
R17362 T22818 T22819 nsubj M-CSF,induces
R17363 T22819 T22819 ROOT induces,induces
R17364 T22820 T22822 amod NF-κB,activity
R17365 T22821 T22822 amod transcriptional,activity
R17366 T22822 T22819 dobj activity,induces
R17367 T22823 T22822 prep in,activity
R17368 T22824 T22823 pobj macrophages,in
R17369 T22825 T22819 punct .,induces
R17370 T22826 T22831 punct (,extracted
R17371 T22827 T22829 nmod A,Nuclei
R17372 T22828 T22827 punct ),A
R17373 T22829 T22831 nsubjpass Nuclei,extracted
R17374 T22830 T22831 auxpass were,extracted
R17375 T22831 T22831 ROOT extracted,extracted
R17376 T22832 T22831 prep from,extracted
R17377 T22833 T22834 amod human,MDMs
R17378 T22834 T22832 pobj MDMs,from
R17379 T22835 T22831 conj treated,extracted
R17380 T22836 T22835 prep without,treated
R17381 T22837 T22836 cc or,without
R17382 T22838 T22836 conj with,without
R17383 T22839 T22838 pobj M-CSF,with
R17384 T22840 T22835 prep for,treated
R17385 T22841 T22844 nummod 15,minutes
R17386 T22842 T22841 cc or,15
R17387 T22843 T22841 conj 30,15
R17388 T22844 T22840 pobj minutes,for
R17389 T22845 T22831 punct .,extracted
R17390 T22846 T22847 compound NF-κB,DNA
R17391 T22847 T22849 compound DNA,activity
R17392 T22848 T22849 amod binding,activity
R17393 T22849 T22851 nsubjpass activity,analyzed
R17394 T22850 T22851 auxpass was,analyzed
R17395 T22851 T22851 ROOT analyzed,analyzed
R17396 T22852 T22851 agent by,analyzed
R17397 T22853 T22854 compound EMSA,Shown
R17398 T22854 T22852 pobj Shown,by
R17399 T22855 T22851 conj is,analyzed
R17400 T22856 T22858 det a,blot
R17401 T22857 T22858 amod representative,blot
R17402 T22858 T22855 attr blot,is
R17403 T22859 T22858 prep from,blot
R17404 T22860 T22862 nummod three,experiments
R17405 T22861 T22862 amod independent,experiments
R17406 T22862 T22859 pobj experiments,from
R17407 T22863 T22851 punct .,analyzed
R17408 T22864 T22864 ROOT NS,NS
R17409 T22865 T22864 punct :,NS
R17410 T22866 T22864 acl non-stimulated,NS
R17411 T22867 T22864 punct .,NS
R17412 T22868 T22874 punct (,transfected
R17413 T22869 T22874 dep B,transfected
R17414 T22870 T22869 punct ),B
R17415 T22871 T22872 compound Human,MDMs
R17416 T22872 T22874 nsubj MDMs,transfected
R17417 T22873 T22874 advmod transiently,transfected
R17418 T22874 T22874 ROOT transfected,transfected
R17419 T22875 T22874 prep with,transfected
R17420 T22876 T22879 amod pTAL-SEAP,constructs
R17421 T22877 T22876 cc or,pTAL-SEAP
R17422 T22878 T22876 conj pNF-κB-SEAP,pTAL-SEAP
R17423 T22879 T22875 pobj constructs,with
R17424 T22880 T22881 auxpass were,treated
R17425 T22881 T22874 dep treated,transfected
R17426 T22882 T22881 prep with,treated
R17427 T22883 T22882 pobj M-CSF,with
R17428 T22884 T22883 prep in,M-CSF
R17429 T22885 T22886 compound X-vivo,medium
R17430 T22886 T22884 pobj medium,in
R17431 T22887 T22881 cc and,treated
R17432 T22888 T22881 conj incubated,treated
R17433 T22889 T22888 prep for,incubated
R17434 T22890 T22891 nummod 6,hours
R17435 T22891 T22889 pobj hours,for
R17436 T22892 T22888 advmod before,incubated
R17437 T22893 T22894 det the,collection
R17438 T22894 T22892 pobj collection,before
R17439 T22895 T22894 prep of,collection
R17440 T22896 T22895 pobj medium,of
R17441 T22897 T22874 punct .,transfected
R17442 T22898 T22899 amod NF-κB,activity
R17443 T22899 T22901 nsubjpass activity,analyzed
R17444 T22900 T22901 auxpass was,analyzed
R17445 T22901 T22901 ROOT analyzed,analyzed
R17446 T22902 T22901 prep by,analyzed
R17447 T22903 T22902 pcomp measuring,by
R17448 T22904 T22905 det the,amount
R17449 T22905 T22903 dobj amount,measuring
R17450 T22906 T22905 prep of,amount
R17451 T22907 T22906 pobj SEAP,of
R17452 T22908 T22901 conj secreted,analyzed
R17453 T22909 T22908 prep into,secreted
R17454 T22910 T22911 det the,medium
R17455 T22911 T22909 pobj medium,into
R17456 T22912 T22911 cc and,medium
R17457 T22913 T22911 conj data,medium
R17458 T22914 T22915 auxpass are,expressed
R17459 T22915 T22915 ROOT expressed,expressed
R17460 T22916 T22915 prep as,expressed
R17461 T22917 T22918 compound fold,increase
R17462 T22918 T22916 pobj increase,as
R17463 T22919 T22918 prep of,increase
R17464 T22920 T22921 compound SEAP,activity
R17465 T22921 T22919 pobj activity,of
R17466 T22922 T22921 prep over,activity
R17467 T22923 T22922 pobj that,over
R17468 T22924 T22921 prep in,activity
R17469 T22925 T22928 amod pTAL-SEAP,cells
R17470 T22926 T22928 amod transfected,cells
R17471 T22927 T22928 amod resting,cells
R17472 T22928 T22924 pobj cells,in
R17473 T22929 T22901 punct .,analyzed
R17474 T22930 T22933 punct (,RAW
R17475 T22931 T22933 nmod C,RAW
R17476 T22932 T22933 punct ),RAW
R17477 T22933 T22938 nsubjpass RAW,transfected
R17478 T22934 T22935 nummod 264.7,cells
R17479 T22935 T22938 nsubjpass cells,transfected
R17480 T22936 T22938 auxpass were,transfected
R17481 T22937 T22938 advmod transiently,transfected
R17482 T22938 T22938 ROOT transfected,transfected
R17483 T22939 T22938 prep with,transfected
R17484 T22940 T22939 pobj pTAL-SEAP,with
R17485 T22941 T22940 cc or,pTAL-SEAP
R17486 T22942 T22940 conj pNF-κB-SEAP,pTAL-SEAP
R17487 T22943 T22938 conj construct,transfected
R17488 T22944 T22938 punct .,transfected
R17489 T22945 T22948 nsubjpass Cells,starved
R17490 T22946 T22948 auxpass were,starved
R17491 T22947 T22948 advmod serum,starved
R17492 T22948 T22948 ROOT starved,starved
R17493 T22949 T22948 prep for,starved
R17494 T22950 T22951 nummod 4,hours
R17495 T22951 T22949 pobj hours,for
R17496 T22952 T22948 advmod prior,starved
R17497 T22953 T22952 prep to,prior
R17498 T22954 T22955 nummod 2,hours
R17499 T22955 T22953 pobj hours,to
R17500 T22956 T22948 advcl stimulation,starved
R17501 T22957 T22956 prep with,stimulation
R17502 T22958 T22960 amod mouse,M-CSF
R17503 T22959 T22960 amod recombinant,M-CSF
R17504 T22960 T22957 pobj M-CSF,with
R17505 T22961 T22963 punct (,ng/ml
R17506 T22962 T22963 nummod 100,ng/ml
R17507 T22963 T22960 appos ng/ml,M-CSF
R17508 T22964 T22963 punct ),ng/ml
R17509 T22965 T22948 punct .,starved
R17510 T22966 T22967 compound Culture,media
R17511 T22967 T22969 nsubjpass media,collected
R17512 T22968 T22969 auxpass was,collected
R17513 T22969 T22969 ROOT collected,collected
R17514 T22970 T22971 aux to,measure
R17515 T22971 T22969 xcomp measure,collected
R17516 T22972 T22973 compound SEAP,production
R17517 T22973 T22971 dobj production,measure
R17518 T22974 T22969 punct .,collected
R17519 T22975 T22977 nsubjpass Data,expressed
R17520 T22976 T22977 auxpass are,expressed
R17521 T22977 T22977 ROOT expressed,expressed
R17522 T22978 T22980 amod mean,S.E.M
R17523 T22979 T22980 compound ±,S.E.M
R17524 T22980 T22977 dobj S.E.M,expressed
R17525 T22981 T22977 punct ",",expressed
R17526 T22982 T22977 prep for,expressed
R17527 T22983 T22985 nummod three,experiments
R17528 T22984 T22985 amod independent,experiments
R17529 T22985 T22982 pobj experiments,for
R17530 T22986 T22977 punct .,expressed

test2

Id Subject Object Predicate Lexical cue
T4653 0-5 Protein denotes M-CSF
T4654 148-153 Protein denotes M-CSF
T4655 253-258 Protein denotes M-CSF
T4656 409-414 Protein denotes M-CSF
T4657 590-595 Protein denotes M-CSF
T4658 938-943 Protein denotes M-CSF
T4659 2016-2021 Protein denotes M-CSF
T4660 2215-2220 Protein denotes M-CSF
T4661 2250-2259 Positive_regulation denotes increased
T4662 2260-2265 Protein denotes NF-κB
T4663 2364-2369 Protein denotes M-CSF
T22805 1001-1006 Protein denotes M-CSF
T22806 1128-1133 Protein denotes M-CSF
T22807 1389-1394 Protein denotes M-CSF
T22808 1842-1847 Protein denotes M-CSF
R3428 T4660 T4661 causeOf M-CSF,increased