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PMC:3245220 / 42666-43653 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T20311 272-274 P0A7Z4 denotes pH
T20312 662-664 P0A7Z4 denotes pH
T20313 899-901 P0A7Z4 denotes pH

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T20325 432-437 Protein denotes NF-κB
T20326 552-571 Protein denotes T4 polynucleokinase

GO-MF

Id Subject Object Predicate Lexical cue
T20323 202-204 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T20324 635-642 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-BP

Id Subject Object Predicate Lexical cue
T20320 202-204 http://purl.obolibrary.org/obo/GO_0033968 denotes CA
T20321 245-250 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T20322 369-374 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T20118 0-15 NN denotes Electrophoresis
T20119 16-18 IN denotes of
T20120 19-22 DT denotes the
T20121 23-31 NN denotes Mobility
T20122 32-37 NN denotes Shift
T20123 38-46 NN denotes Analysis
T20124 47-48 -LRB- denotes (
T20125 48-52 NN denotes EMSA
T20126 52-53 -RRB- denotes )
T20127 54-61 JJ denotes Nuclear
T20128 62-70 NN denotes extracts
T20129 71-75 VB denotes were
T20130 76-84 VB denotes isolated
T20131 85-89 IN denotes from
T20132 90-94 NN denotes MDMs
T20133 95-97 IN denotes by
T20134 98-103 VB denotes using
T20135 104-105 DT denotes a
T20136 106-113 JJ denotes nuclear
T20137 114-124 NN denotes extraction
T20138 125-128 NN denotes kit
T20139 129-138 VB denotes according
T20140 139-141 TO denotes to
T20141 142-145 DT denotes the
T20142 146-158 NN denotes manufacturer
T20143 158-160 POS denotes 's
T20144 161-172 NN denotes instruction
T20145 173-177 IN denotes from
T20146 178-184 JJ denotes Active
T20147 185-190 NNP denotes Motif
T20148 191-192 -LRB- denotes (
T20149 192-200 NNP denotes Carlsbad
T20150 200-201 -COMMA- denotes ,
T20151 202-204 NNP denotes CA
T20152 204-205 -RRB- denotes )
T20153 207-214 RB denotes Briefly
T20154 214-215 -COMMA- denotes ,
T20155 216-220 NN denotes MDMs
T20156 221-225 VB denotes were
T20157 226-231 VB denotes lysed
T20158 232-234 IN denotes in
T20159 235-244 JJ denotes hypotonic
T20160 245-250 NN denotes lysis
T20161 251-257 NN denotes buffer
T20162 258-259 -LRB- denotes (
T20163 259-261 CD denotes 20
T20164 262-264 NN denotes mM
T20165 265-270 NNP denotes Hepes
T20166 270-271 -COMMA- denotes ,
T20167 272-274 NN denotes pH
T20168 275-278 CD denotes 7.5
T20169 278-279 -COLON- denotes ;
T20170 280-281 CD denotes 5
T20171 282-284 NN denotes mM
T20172 285-288 NN denotes NaF
T20173 288-289 -COMMA- denotes ,
T20174 290-292 CD denotes 10
T20175 293-295 NN denotes µM
T20176 296-303 NN denotes Na2MoO4
T20177 304-307 CD denotes 0.5
T20178 307-308 NN denotes %
T20179 309-314 NN denotes NP-40
T20180 315-318 CC denotes and
T20181 319-322 CD denotes 0.1
T20182 323-325 NN denotes mM
T20183 326-330 NN denotes EDTA
T20184 330-331 -RRB- denotes )
T20185 331-332 -COMMA- denotes ,
T20186 333-336 CC denotes and
T20187 337-341 RB denotes then
T20188 342-348 NN denotes nuclei
T20189 349-353 VB denotes were
T20190 354-365 VB denotes resuspended
T20191 366-368 IN denotes in
T20192 369-374 NN denotes lysis
T20193 375-381 NN denotes buffer
T20194 382-394 VB denotes supplemented
T20195 395-399 IN denotes with
T20196 400-403 CD denotes 0.5
T20197 404-406 NN denotes mM
T20198 407-410 NN denotes DTT
T20199 411-414 CC denotes and
T20200 415-418 CD denotes 0.2
T20201 419-421 NN denotes mM
T20202 422-426 NN denotes PMSF
T20203 428-431 DT denotes The
T20204 432-437 NN denotes NF-κB
T20205 438-447 NN denotes consensus
T20206 448-464 NN denotes oligonucleotides
T20207 465-466 -LRB- denotes (
T20208 466-471 NN denotes sense
T20209 471-472 -COLON- denotes :
T20210 473-495 NN denotes AGTTGAGGGGACTTTCCCAGGC
T20211 495-496 -COLON- denotes ;
T20212 497-506 JJ denotes antisense
T20213 506-507 -COLON- denotes :
T20214 508-530 NN denotes GCCTGGGAAAGTCCCCTCAACT
T20215 530-531 -RRB- denotes )
T20216 532-539 VB denotes labeled
T20217 540-544 IN denotes with
T20218 545-548 NN denotes 32P
T20219 549-551 IN denotes by
T20220 552-554 NN denotes T4
T20221 555-571 NN denotes polynucleokinase
T20222 572-573 -LRB- denotes (
T20223 573-580 NNP denotes Promega
T20224 580-581 -COMMA- denotes ,
T20225 582-589 NNP denotes Madison
T20226 589-590 -COMMA- denotes ,
T20227 591-593 NNP denotes WI
T20228 593-594 -RRB- denotes )
T20229 595-599 VB denotes were
T20230 600-609 VB denotes incubated
T20231 610-614 IN denotes with
T20232 615-622 JJ denotes nuclear
T20233 623-631 NN denotes extracts
T20234 632-634 IN denotes in
T20235 635-642 NN denotes binding
T20236 643-649 NN denotes buffer
T20237 650-651 -LRB- denotes (
T20238 651-653 CD denotes 10
T20239 654-656 NN denotes mM
T20240 657-661 NN denotes Tris
T20241 662-664 NN denotes pH
T20242 665-668 CD denotes 7.6
T20243 668-669 -COMMA- denotes ,
T20244 670-671 CD denotes 1
T20245 672-674 NN denotes mM
T20246 675-678 NN denotes DTT
T20247 678-679 -COMMA- denotes ,
T20248 680-683 CD denotes 0.5
T20249 684-686 NN denotes mM
T20250 687-691 NN denotes EDTA
T20251 691-692 -COMMA- denotes ,
T20252 693-694 CD denotes 2
T20253 695-697 NN denotes µg
T20254 698-707 NN denotes polydI-dC
T20255 708-711 CC denotes and
T20256 712-714 CD denotes 10
T20257 714-715 NN denotes %
T20258 716-724 NN denotes Glycerol
T20259 724-725 -RRB- denotes )
T20260 726-728 IN denotes at
T20261 729-733 NN denotes 30°C
T20262 734-737 IN denotes for
T20263 738-740 CD denotes 30
T20264 741-748 NN denotes minutes
T20265 750-753 DT denotes The
T20266 754-758 JJ denotes free
T20267 759-762 NN denotes DNA
T20268 763-766 CC denotes and
T20269 767-778 JJ denotes DNA-protein
T20270 779-787 NN denotes mixtures
T20271 788-792 VB denotes were
T20272 793-801 VB denotes resolved
T20273 802-804 IN denotes in
T20274 805-806 CD denotes 5
T20275 806-807 NN denotes %
T20276 808-814 JJ denotes native
T20277 815-829 NN denotes polyacrylamide
T20278 830-834 NN denotes gels
T20279 835-837 IN denotes in
T20280 838-842 CD denotes 0.5×
T20281 843-846 NN denotes TBE
T20282 847-853 NN denotes buffer
T20283 854-855 -LRB- denotes (
T20284 855-857 CD denotes 45
T20285 858-860 NN denotes mM
T20286 861-865 NNP denotes Tris
T20287 865-866 -COMMA- denotes ,
T20288 867-869 CD denotes 45
T20289 870-872 NN denotes mM
T20290 873-878 JJ denotes boric
T20291 879-883 NN denotes acid
T20292 884-887 CC denotes and
T20293 888-889 CD denotes 1
T20294 890-892 NN denotes mM
T20295 893-897 NN denotes EDTA
T20296 897-898 -COMMA- denotes ,
T20297 899-901 NN denotes pH
T20298 902-905 CD denotes 8.3
T20299 905-906 -RRB- denotes )
T20300 907-909 IN denotes by
T20301 910-925 NN denotes electrophoresis
T20302 927-930 DT denotes The
T20303 931-934 NN denotes gel
T20304 935-938 VB denotes was
T20305 939-944 VB denotes dried
T20306 945-948 CC denotes and
T20307 949-958 VB denotes subjected
T20308 959-961 TO denotes to
T20309 962-977 NN denotes autoradiography
T20310 978-986 NN denotes analysis
R15079 T20118 T20119 arg1Of Electrophoresis,of
R15080 T20123 T20119 arg2Of Analysis,of
R15081 T20123 T20120 arg1Of Analysis,the
R15082 T20123 T20121 arg1Of Analysis,Mobility
R15083 T20123 T20122 arg1Of Analysis,Shift
R15084 T20123 T20124 arg1Of Analysis,(
R15085 T20125 T20124 arg2Of EMSA,(
R15086 T20126 T20124 arg3Of ),(
R15087 T20128 T20127 arg1Of extracts,Nuclear
R15088 T20128 T20129 arg1Of extracts,were
R15089 T20128 T20130 arg2Of extracts,isolated
R15090 T20128 T20134 arg1Of extracts,using
R15091 T20130 T20129 arg2Of isolated,were
R15092 T20130 T20131 arg1Of isolated,from
R15093 T20130 T20133 arg1Of isolated,by
R15094 T20132 T20131 arg2Of MDMs,from
R15095 T20134 T20133 arg2Of using,by
R15096 T20134 T20139 arg1Of using,according
R15097 T20134 T20145 arg1Of using,from
R15098 T20138 T20134 arg2Of kit,using
R15099 T20138 T20135 arg1Of kit,a
R15100 T20138 T20136 arg1Of kit,nuclear
R15101 T20138 T20137 arg1Of kit,extraction
R15102 T20140 T20139 arg2Of to,according
R15103 T20142 T20141 arg1Of manufacturer,the
R15104 T20142 T20143 arg2Of manufacturer,'s
R15105 T20144 T20140 arg2Of instruction,to
R15106 T20144 T20143 arg1Of instruction,'s
R15107 T20147 T20145 arg2Of Motif,from
R15108 T20147 T20146 arg1Of Motif,Active
R15109 T20147 T20148 arg1Of Motif,(
R15110 T20149 T20148 arg2Of Carlsbad,(
R15111 T20149 T20150 arg1Of Carlsbad,","
R15112 T20149 T20151 arg1Of Carlsbad,CA
R15113 T20152 T20148 arg3Of ),(
R15114 T20155 T20156 arg1Of MDMs,were
R15115 T20155 T20157 arg2Of MDMs,lysed
R15116 T20157 T20156 arg2Of lysed,were
R15117 T20157 T20158 arg1Of lysed,in
R15118 T20157 T20162 arg1Of lysed,(
R15119 T20157 T20186 arg1Of lysed,and
R15120 T20161 T20158 arg2Of buffer,in
R15121 T20161 T20159 arg1Of buffer,hypotonic
R15122 T20161 T20160 arg1Of buffer,lysis
R15123 T20165 T20163 arg1Of Hepes,20
R15124 T20165 T20164 arg1Of Hepes,mM
R15125 T20165 T20166 arg1Of Hepes,","
R15126 T20165 T20173 arg1Of Hepes,","
R15127 T20167 T20168 arg1Of pH,7.5
R15128 T20167 T20169 arg1Of pH,;
R15129 T20169 T20166 arg2Of ;,","
R15130 T20170 T20171 arg1Of 5,mM
R15131 T20172 T20169 arg2Of NaF,;
R15132 T20172 T20170 arg1Of NaF,5
R15133 T20173 T20180 arg1Of ",",and
R15134 T20177 T20178 arg1Of 0.5,%
R15135 T20179 T20173 arg2Of NP-40,","
R15136 T20179 T20174 arg1Of NP-40,10
R15137 T20179 T20175 arg1Of NP-40,µM
R15138 T20179 T20176 arg1Of NP-40,Na2MoO4
R15139 T20179 T20177 arg1Of NP-40,0.5
R15140 T20180 T20162 arg2Of and,(
R15141 T20181 T20182 arg1Of 0.1,mM
R15142 T20183 T20180 arg2Of EDTA,and
R15143 T20183 T20181 arg1Of EDTA,0.1
R15144 T20184 T20162 arg3Of ),(
R15145 T20186 T20153 arg1Of and,Briefly
R15146 T20186 T20154 arg1Of and,","
R15147 T20186 T20185 arg1Of and,","
R15148 T20188 T20189 arg1Of nuclei,were
R15149 T20188 T20190 arg2Of nuclei,resuspended
R15150 T20190 T20186 arg2Of resuspended,and
R15151 T20190 T20187 arg1Of resuspended,then
R15152 T20190 T20189 arg2Of resuspended,were
R15153 T20190 T20191 arg1Of resuspended,in
R15154 T20193 T20191 arg2Of buffer,in
R15155 T20193 T20192 arg1Of buffer,lysis
R15156 T20193 T20194 arg2Of buffer,supplemented
R15157 T20194 T20195 arg1Of supplemented,with
R15158 T20196 T20197 arg1Of 0.5,mM
R15159 T20198 T20196 arg1Of DTT,0.5
R15160 T20198 T20199 arg1Of DTT,and
R15161 T20199 T20195 arg2Of and,with
R15162 T20200 T20201 arg1Of 0.2,mM
R15163 T20202 T20199 arg2Of PMSF,and
R15164 T20202 T20200 arg1Of PMSF,0.2
R15165 T20206 T20203 arg1Of oligonucleotides,The
R15166 T20206 T20204 arg1Of oligonucleotides,NF-κB
R15167 T20206 T20205 arg1Of oligonucleotides,consensus
R15168 T20206 T20207 arg1Of oligonucleotides,(
R15169 T20206 T20216 arg2Of oligonucleotides,labeled
R15170 T20206 T20229 arg1Of oligonucleotides,were
R15171 T20206 T20230 arg2Of oligonucleotides,incubated
R15172 T20208 T20209 arg1Of sense,:
R15173 T20209 T20207 arg2Of :,(
R15174 T20209 T20211 arg1Of :,;
R15175 T20210 T20209 arg2Of AGTTGAGGGGACTTTCCCAGGC,:
R15176 T20212 T20213 arg1Of antisense,:
R15177 T20214 T20211 arg2Of GCCTGGGAAAGTCCCCTCAACT,;
R15178 T20214 T20212 arg1Of GCCTGGGAAAGTCCCCTCAACT,antisense
R15179 T20215 T20207 arg3Of ),(
R15180 T20216 T20217 arg1Of labeled,with
R15181 T20216 T20222 arg1Of labeled,(
R15182 T20218 T20217 arg2Of 32P,with
R15183 T20218 T20219 arg1Of 32P,by
R15184 T20221 T20219 arg2Of polynucleokinase,by
R15185 T20221 T20220 arg1Of polynucleokinase,T4
R15186 T20223 T20224 arg1Of Promega,","
R15187 T20224 T20222 arg2Of ",",(
R15188 T20224 T20226 arg1Of ",",","
R15189 T20225 T20224 arg2Of Madison,","
R15190 T20227 T20226 arg2Of WI,","
R15191 T20228 T20222 arg3Of ),(
R15192 T20230 T20229 arg2Of incubated,were
R15193 T20230 T20231 arg1Of incubated,with
R15194 T20230 T20262 arg1Of incubated,for
R15195 T20233 T20231 arg2Of extracts,with
R15196 T20233 T20232 arg1Of extracts,nuclear
R15197 T20233 T20234 arg1Of extracts,in
R15198 T20233 T20237 arg1Of extracts,(
R15199 T20233 T20260 arg1Of extracts,at
R15200 T20236 T20234 arg2Of buffer,in
R15201 T20236 T20235 arg1Of buffer,binding
R15202 T20238 T20239 arg1Of 10,mM
R15203 T20241 T20240 arg1Of pH,Tris
R15204 T20241 T20242 arg1Of pH,7.6
R15205 T20241 T20243 arg1Of pH,","
R15206 T20243 T20247 arg1Of ",",","
R15207 T20244 T20245 arg1Of 1,mM
R15208 T20246 T20243 arg2Of DTT,","
R15209 T20246 T20244 arg1Of DTT,1
R15210 T20247 T20251 arg1Of ",",","
R15211 T20248 T20249 arg1Of 0.5,mM
R15212 T20250 T20247 arg2Of EDTA,","
R15213 T20250 T20248 arg1Of EDTA,0.5
R15214 T20251 T20238 arg1Of ",",10
R15215 T20251 T20255 arg1Of ",",and
R15216 T20252 T20253 arg1Of 2,µg
R15217 T20254 T20251 arg2Of polydI-dC,","
R15218 T20254 T20252 arg1Of polydI-dC,2
R15219 T20255 T20237 arg2Of and,(
R15220 T20256 T20257 arg1Of 10,%
R15221 T20258 T20255 arg2Of Glycerol,and
R15222 T20258 T20256 arg1Of Glycerol,10
R15223 T20259 T20237 arg3Of ),(
R15224 T20261 T20260 arg2Of 30°C,at
R15225 T20264 T20262 arg2Of minutes,for
R15226 T20264 T20263 arg1Of minutes,30
R15227 T20267 T20266 arg1Of DNA,free
R15228 T20267 T20268 arg1Of DNA,and
R15229 T20268 T20265 arg1Of and,The
R15230 T20268 T20271 arg1Of and,were
R15231 T20268 T20272 arg2Of and,resolved
R15232 T20270 T20268 arg2Of mixtures,and
R15233 T20270 T20269 arg1Of mixtures,DNA-protein
R15234 T20272 T20271 arg2Of resolved,were
R15235 T20272 T20273 arg1Of resolved,in
R15236 T20274 T20275 arg1Of 5,%
R15237 T20278 T20273 arg2Of gels,in
R15238 T20278 T20274 arg1Of gels,5
R15239 T20278 T20276 arg1Of gels,native
R15240 T20278 T20277 arg1Of gels,polyacrylamide
R15241 T20278 T20279 arg1Of gels,in
R15242 T20278 T20283 arg1Of gels,(
R15243 T20278 T20300 arg1Of gels,by
R15244 T20282 T20279 arg2Of buffer,in
R15245 T20282 T20280 arg1Of buffer,0.5×
R15246 T20282 T20281 arg1Of buffer,TBE
R15247 T20286 T20283 arg2Of Tris,(
R15248 T20286 T20284 arg1Of Tris,45
R15249 T20286 T20285 arg1Of Tris,mM
R15250 T20286 T20287 arg1Of Tris,","
R15251 T20286 T20296 arg1Of Tris,","
R15252 T20291 T20288 arg1Of acid,45
R15253 T20291 T20289 arg1Of acid,mM
R15254 T20291 T20290 arg1Of acid,boric
R15255 T20291 T20292 arg1Of acid,and
R15256 T20292 T20287 arg2Of and,","
R15257 T20293 T20294 arg1Of 1,mM
R15258 T20295 T20292 arg2Of EDTA,and
R15259 T20295 T20293 arg1Of EDTA,1
R15260 T20297 T20296 arg2Of pH,","
R15261 T20297 T20298 arg1Of pH,8.3
R15262 T20299 T20283 arg3Of ),(
R15263 T20301 T20300 arg2Of electrophoresis,by
R15264 T20303 T20302 arg1Of gel,The
R15265 T20303 T20304 arg1Of gel,was
R15266 T20303 T20305 arg2Of gel,dried
R15267 T20303 T20307 arg2Of gel,subjected
R15268 T20305 T20306 arg1Of dried,and
R15269 T20306 T20304 arg2Of and,was
R15270 T20307 T20306 arg2Of subjected,and
R15271 T20307 T20308 arg1Of subjected,to
R15272 T20310 T20308 arg2Of analysis,to
R15273 T20310 T20309 arg1Of analysis,autoradiography

sentences

Id Subject Object Predicate Lexical cue
T19911 0-53 Sentence denotes Electrophoresis of the Mobility Shift Analysis (EMSA)
T19912 54-206 Sentence denotes Nuclear extracts were isolated from MDMs by using a nuclear extraction kit according to the manufacturer's instruction from Active Motif (Carlsbad, CA).
T19913 207-427 Sentence denotes Briefly, MDMs were lysed in hypotonic lysis buffer (20 mM Hepes, pH 7.5; 5 mM NaF, 10 µM Na2MoO4 0.5% NP-40 and 0.1 mM EDTA), and then nuclei were resuspended in lysis buffer supplemented with 0.5 mM DTT and 0.2 mM PMSF.
T19914 428-749 Sentence denotes The NF-κB consensus oligonucleotides (sense: AGTTGAGGGGACTTTCCCAGGC; antisense: GCCTGGGAAAGTCCCCTCAACT) labeled with 32P by T4 polynucleokinase (Promega, Madison, WI) were incubated with nuclear extracts in binding buffer (10 mM Tris pH 7.6, 1 mM DTT, 0.5 mM EDTA, 2 µg polydI-dC and 10% Glycerol) at 30°C for 30 minutes.
T19915 750-926 Sentence denotes The free DNA and DNA-protein mixtures were resolved in 5% native polyacrylamide gels in 0.5× TBE buffer (45 mM Tris, 45 mM boric acid and 1 mM EDTA, pH 8.3) by electrophoresis.
T19916 927-987 Sentence denotes The gel was dried and subjected to autoradiography analysis.
T296 0-53 Sentence denotes Electrophoresis of the Mobility Shift Analysis (EMSA)
T297 54-206 Sentence denotes Nuclear extracts were isolated from MDMs by using a nuclear extraction kit according to the manufacturer's instruction from Active Motif (Carlsbad, CA).
T298 207-427 Sentence denotes Briefly, MDMs were lysed in hypotonic lysis buffer (20 mM Hepes, pH 7.5; 5 mM NaF, 10 µM Na2MoO4 0.5% NP-40 and 0.1 mM EDTA), and then nuclei were resuspended in lysis buffer supplemented with 0.5 mM DTT and 0.2 mM PMSF.
T299 428-749 Sentence denotes The NF-κB consensus oligonucleotides (sense: AGTTGAGGGGACTTTCCCAGGC; antisense: GCCTGGGAAAGTCCCCTCAACT) labeled with 32P by T4 polynucleokinase (Promega, Madison, WI) were incubated with nuclear extracts in binding buffer (10 mM Tris pH 7.6, 1 mM DTT, 0.5 mM EDTA, 2 µg polydI-dC and 10% Glycerol) at 30°C for 30 minutes.
T300 750-926 Sentence denotes The free DNA and DNA-protein mixtures were resolved in 5% native polyacrylamide gels in 0.5× TBE buffer (45 mM Tris, 45 mM boric acid and 1 mM EDTA, pH 8.3) by electrophoresis.
T301 927-987 Sentence denotes The gel was dried and subjected to autoradiography analysis.

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T19917 0-15 NNP denotes Electrophoresis
T19918 16-18 IN denotes of
T19919 19-22 DT denotes the
T19920 23-31 NNP denotes Mobility
T19921 32-37 NNP denotes Shift
T19922 38-46 NNP denotes Analysis
T19923 47-48 -LRB- denotes (
T19924 48-52 NNP denotes EMSA
T19925 52-53 -RRB- denotes )
T19926 54-61 NNP denotes Nuclear
T19927 62-70 NNS denotes extracts
T19928 71-75 VBD denotes were
T19929 76-84 VBN denotes isolated
T19930 85-89 IN denotes from
T19931 90-94 NNS denotes MDMs
T19932 95-97 IN denotes by
T19933 98-103 VBG denotes using
T19934 104-105 DT denotes a
T19935 106-113 JJ denotes nuclear
T19936 114-124 NN denotes extraction
T19937 125-128 NN denotes kit
T19938 129-138 VBG denotes according
T19939 139-141 TO denotes to
T19940 142-145 DT denotes the
T19941 146-158 NN denotes manufacturer
T19942 158-160 POS denotes 's
T19943 161-172 NN denotes instruction
T19944 173-177 IN denotes from
T19945 178-184 NNP denotes Active
T19946 185-190 NNP denotes Motif
T19947 191-192 -LRB- denotes (
T19948 192-200 NNP denotes Carlsbad
T19949 200-201 , denotes ,
T19950 202-204 NNP denotes CA
T19951 204-205 -RRB- denotes )
T19952 205-206 . denotes .
T19953 207-214 RB denotes Briefly
T19954 214-215 , denotes ,
T19955 216-220 NNS denotes MDMs
T19956 221-225 VBD denotes were
T19957 226-231 VBN denotes lysed
T19958 232-234 IN denotes in
T19959 235-244 JJ denotes hypotonic
T19960 245-250 NN denotes lysis
T19961 251-257 NN denotes buffer
T19962 258-259 -LRB- denotes (
T19963 259-261 CD denotes 20
T19964 262-264 NNP denotes mM
T19965 265-270 NNP denotes Hepes
T19966 270-271 , denotes ,
T19967 272-274 NNP denotes pH
T19968 275-278 CD denotes 7.5
T19969 278-279 : denotes ;
T19970 280-281 CD denotes 5
T19971 282-284 NNP denotes mM
T19972 285-288 NNP denotes NaF
T19973 288-289 , denotes ,
T19974 290-292 CD denotes 10
T19975 293-295 NNP denotes µM
T19976 296-303 NNP denotes Na2MoO4
T19977 304-307 CD denotes 0.5
T19978 307-308 NN denotes %
T19979 309-314 NN denotes NP-40
T19980 315-318 CC denotes and
T19981 319-322 CD denotes 0.1
T19982 323-325 NNP denotes mM
T19983 326-330 NNP denotes EDTA
T19984 330-331 -RRB- denotes )
T19985 331-332 , denotes ,
T19986 333-336 CC denotes and
T19987 337-341 RB denotes then
T19988 342-348 NNS denotes nuclei
T19989 349-353 VBD denotes were
T19990 354-365 VBN denotes resuspended
T19991 366-368 IN denotes in
T19992 369-374 NN denotes lysis
T19993 375-381 NN denotes buffer
T19994 382-394 VBN denotes supplemented
T19995 395-399 IN denotes with
T19996 400-403 CD denotes 0.5
T19997 404-406 NNP denotes mM
T19998 407-410 NNP denotes DTT
T19999 411-414 CC denotes and
T20000 415-418 CD denotes 0.2
T20001 419-421 NNP denotes mM
T20002 422-426 NNP denotes PMSF
T20003 426-427 . denotes .
T20004 428-431 DT denotes The
T20005 432-437 JJ denotes NF-κB
T20006 438-447 NN denotes consensus
T20007 448-464 NNS denotes oligonucleotides
T20008 465-466 -LRB- denotes (
T20009 466-471 NN denotes sense
T20010 471-472 : denotes :
T20011 473-495 NNP denotes AGTTGAGGGGACTTTCCCAGGC
T20012 495-496 : denotes ;
T20013 497-506 NN denotes antisense
T20014 506-507 : denotes :
T20015 508-530 NNP denotes GCCTGGGAAAGTCCCCTCAACT
T20016 530-531 -RRB- denotes )
T20017 532-539 VBN denotes labeled
T20018 540-544 IN denotes with
T20019 545-548 CD denotes 32P
T20020 549-551 IN denotes by
T20021 552-554 CD denotes T4
T20022 555-571 NN denotes polynucleokinase
T20023 572-573 -LRB- denotes (
T20024 573-580 NNP denotes Promega
T20025 580-581 , denotes ,
T20026 582-589 NNP denotes Madison
T20027 589-590 , denotes ,
T20028 591-593 NNP denotes WI
T20029 593-594 -RRB- denotes )
T20030 595-599 VBD denotes were
T20031 600-609 VBN denotes incubated
T20032 610-614 IN denotes with
T20033 615-622 JJ denotes nuclear
T20034 623-631 NNS denotes extracts
T20035 632-634 IN denotes in
T20036 635-642 JJ denotes binding
T20037 643-649 NN denotes buffer
T20038 650-651 -LRB- denotes (
T20039 651-653 CD denotes 10
T20040 654-656 NNP denotes mM
T20041 657-661 NNP denotes Tris
T20042 662-664 NNP denotes pH
T20043 665-668 CD denotes 7.6
T20044 668-669 , denotes ,
T20045 670-671 CD denotes 1
T20046 672-674 NNP denotes mM
T20047 675-678 NNP denotes DTT
T20048 678-679 , denotes ,
T20049 680-683 CD denotes 0.5
T20050 684-686 NNP denotes mM
T20051 687-691 NNP denotes EDTA
T20052 691-692 , denotes ,
T20053 693-694 CD denotes 2
T20054 695-697 NN denotes µg
T20055 698-707 NN denotes polydI-dC
T20056 708-711 CC denotes and
T20057 712-714 CD denotes 10
T20058 714-715 NN denotes %
T20059 716-724 NNP denotes Glycerol
T20060 724-725 -RRB- denotes )
T20061 726-728 IN denotes at
T20062 729-731 CD denotes 30
T20063 731-732 CD denotes °
T20064 732-733 NNP denotes C
T20065 734-737 IN denotes for
T20066 738-740 CD denotes 30
T20067 741-748 NNS denotes minutes
T20068 748-749 . denotes .
T20069 750-753 DT denotes The
T20070 754-758 JJ denotes free
T20071 759-762 NN denotes DNA
T20072 763-766 CC denotes and
T20073 767-778 JJ denotes DNA-protein
T20074 779-787 NNS denotes mixtures
T20075 788-792 VBD denotes were
T20076 793-801 VBN denotes resolved
T20077 802-804 IN denotes in
T20078 805-806 CD denotes 5
T20079 806-807 NN denotes %
T20080 808-814 JJ denotes native
T20081 815-829 NN denotes polyacrylamide
T20082 830-834 NNS denotes gels
T20083 835-837 IN denotes in
T20084 838-841 CD denotes 0.5
T20085 841-842 CD denotes ×
T20086 843-846 NNP denotes TBE
T20087 847-853 NN denotes buffer
T20088 854-855 -LRB- denotes (
T20089 855-857 CD denotes 45
T20090 858-860 NNP denotes mM
T20091 861-865 NNP denotes Tris
T20092 865-866 , denotes ,
T20093 867-869 CD denotes 45
T20094 870-872 NN denotes mM
T20095 873-878 JJ denotes boric
T20096 879-883 NN denotes acid
T20097 884-887 CC denotes and
T20098 888-889 CD denotes 1
T20099 890-892 NNP denotes mM
T20100 893-897 NNP denotes EDTA
T20101 897-898 , denotes ,
T20102 899-901 NNP denotes pH
T20103 902-905 CD denotes 8.3
T20104 905-906 -RRB- denotes )
T20105 907-909 IN denotes by
T20106 910-925 NN denotes electrophoresis
T20107 925-926 . denotes .
T20108 927-930 DT denotes The
T20109 931-934 NN denotes gel
T20110 935-938 VBD denotes was
T20111 939-944 VBN denotes dried
T20112 945-948 CC denotes and
T20113 949-958 VBN denotes subjected
T20114 959-961 TO denotes to
T20115 962-977 NN denotes autoradiography
T20116 978-986 NN denotes analysis
T20117 986-987 . denotes .
R14878 T19917 T19927 nmod Electrophoresis,extracts
R14879 T19918 T19917 prep of,Electrophoresis
R14880 T19919 T19922 det the,Analysis
R14881 T19920 T19922 compound Mobility,Analysis
R14882 T19921 T19922 compound Shift,Analysis
R14883 T19922 T19918 pobj Analysis,of
R14884 T19923 T19922 punct (,Analysis
R14885 T19924 T19922 appos EMSA,Analysis
R14886 T19925 T19922 punct ),Analysis
R14887 T19926 T19927 compound Nuclear,extracts
R14888 T19927 T19929 nsubjpass extracts,isolated
R14889 T19928 T19929 auxpass were,isolated
R14890 T19929 T19929 ROOT isolated,isolated
R14891 T19930 T19929 prep from,isolated
R14892 T19931 T19930 pobj MDMs,from
R14893 T19932 T19929 prep by,isolated
R14894 T19933 T19932 pcomp using,by
R14895 T19934 T19937 det a,kit
R14896 T19935 T19936 amod nuclear,extraction
R14897 T19936 T19937 compound extraction,kit
R14898 T19937 T19933 dobj kit,using
R14899 T19938 T19929 prep according,isolated
R14900 T19939 T19938 prep to,according
R14901 T19940 T19941 det the,manufacturer
R14902 T19941 T19943 poss manufacturer,instruction
R14903 T19942 T19941 case 's,manufacturer
R14904 T19943 T19939 pobj instruction,to
R14905 T19944 T19943 prep from,instruction
R14906 T19945 T19946 compound Active,Motif
R14907 T19946 T19944 pobj Motif,from
R14908 T19947 T19951 punct (,)
R14909 T19948 T19951 nmod Carlsbad,)
R14910 T19949 T19948 punct ",",Carlsbad
R14911 T19950 T19948 conj CA,Carlsbad
R14912 T19951 T19929 punct ),isolated
R14913 T19952 T19929 punct .,isolated
R14914 T19953 T19957 advmod Briefly,lysed
R14915 T19954 T19957 punct ",",lysed
R14916 T19955 T19957 nsubjpass MDMs,lysed
R14917 T19956 T19957 auxpass were,lysed
R14918 T19957 T19957 ROOT lysed,lysed
R14919 T19958 T19957 prep in,lysed
R14920 T19959 T19961 amod hypotonic,buffer
R14921 T19960 T19961 compound lysis,buffer
R14922 T19961 T19958 pobj buffer,in
R14923 T19962 T19961 punct (,buffer
R14924 T19963 T19965 nummod 20,Hepes
R14925 T19964 T19965 compound mM,Hepes
R14926 T19965 T19961 appos Hepes,buffer
R14927 T19966 T19965 punct ",",Hepes
R14928 T19967 T19965 appos pH,Hepes
R14929 T19968 T19967 nummod 7.5,pH
R14930 T19969 T19965 punct ;,Hepes
R14931 T19970 T19972 nummod 5,NaF
R14932 T19971 T19972 compound mM,NaF
R14933 T19972 T19965 appos NaF,Hepes
R14934 T19973 T19972 punct ",",NaF
R14935 T19974 T19979 nummod 10,NP-40
R14936 T19975 T19976 compound µM,Na2MoO4
R14937 T19976 T19979 compound Na2MoO4,NP-40
R14938 T19977 T19978 nummod 0.5,%
R14939 T19978 T19979 compound %,NP-40
R14940 T19979 T19972 appos NP-40,NaF
R14941 T19980 T19979 cc and,NP-40
R14942 T19981 T19983 nummod 0.1,EDTA
R14943 T19982 T19983 compound mM,EDTA
R14944 T19983 T19979 conj EDTA,NP-40
R14945 T19984 T19983 punct ),EDTA
R14946 T19985 T19972 punct ",",NaF
R14947 T19986 T19972 cc and,NaF
R14948 T19987 T19990 advmod then,resuspended
R14949 T19988 T19990 nsubjpass nuclei,resuspended
R14950 T19989 T19990 auxpass were,resuspended
R14951 T19990 T19957 conj resuspended,lysed
R14952 T19991 T19990 prep in,resuspended
R14953 T19992 T19993 compound lysis,buffer
R14954 T19993 T19991 pobj buffer,in
R14955 T19994 T19990 conj supplemented,resuspended
R14956 T19995 T19994 prep with,supplemented
R14957 T19996 T19998 nummod 0.5,DTT
R14958 T19997 T19998 compound mM,DTT
R14959 T19998 T19995 pobj DTT,with
R14960 T19999 T19998 cc and,DTT
R14961 T20000 T20002 nummod 0.2,PMSF
R14962 T20001 T20002 compound mM,PMSF
R14963 T20002 T19998 conj PMSF,DTT
R14964 T20003 T19990 punct .,resuspended
R14965 T20004 T20031 det The,incubated
R14966 T20005 T20006 amod NF-κB,consensus
R14967 T20006 T20031 nsubjpass consensus,incubated
R14968 T20007 T20006 appos oligonucleotides,consensus
R14969 T20008 T20007 punct (,oligonucleotides
R14970 T20009 T20006 conj sense,consensus
R14971 T20010 T20006 punct :,consensus
R14972 T20011 T20006 appos AGTTGAGGGGACTTTCCCAGGC,consensus
R14973 T20012 T20006 punct ;,consensus
R14974 T20013 T20017 nsubj antisense,labeled
R14975 T20014 T20013 punct :,antisense
R14976 T20015 T20013 appos GCCTGGGAAAGTCCCCTCAACT,antisense
R14977 T20016 T20013 punct ),antisense
R14978 T20017 T20006 acl labeled,consensus
R14979 T20018 T20017 prep with,labeled
R14980 T20019 T20018 pobj 32P,with
R14981 T20020 T20017 agent by,labeled
R14982 T20021 T20022 nummod T4,polynucleokinase
R14983 T20022 T20020 pobj polynucleokinase,by
R14984 T20023 T20024 punct (,Promega
R14985 T20024 T20022 appos Promega,polynucleokinase
R14986 T20025 T20024 punct ",",Promega
R14987 T20026 T20024 conj Madison,Promega
R14988 T20027 T20026 punct ",",Madison
R14989 T20028 T20026 conj WI,Madison
R14990 T20029 T20024 punct ),Promega
R14991 T20030 T20031 auxpass were,incubated
R14992 T20031 T20031 ROOT incubated,incubated
R14993 T20032 T20031 prep with,incubated
R14994 T20033 T20034 amod nuclear,extracts
R14995 T20034 T20032 pobj extracts,with
R14996 T20035 T20034 prep in,extracts
R14997 T20036 T20037 amod binding,buffer
R14998 T20037 T20035 pobj buffer,in
R14999 T20038 T20034 punct (,extracts
R15000 T20039 T20042 nummod 10,pH
R15001 T20040 T20042 compound mM,pH
R15002 T20041 T20042 compound Tris,pH
R15003 T20042 T20034 appos pH,extracts
R15004 T20043 T20047 nummod 7.6,DTT
R15005 T20044 T20047 punct ",",DTT
R15006 T20045 T20047 nummod 1,DTT
R15007 T20046 T20047 compound mM,DTT
R15008 T20047 T20034 appos DTT,extracts
R15009 T20048 T20047 punct ",",DTT
R15010 T20049 T20051 nummod 0.5,EDTA
R15011 T20050 T20051 compound mM,EDTA
R15012 T20051 T20047 conj EDTA,DTT
R15013 T20052 T20051 punct ",",EDTA
R15014 T20053 T20054 nummod 2,µg
R15015 T20054 T20055 compound µg,polydI-dC
R15016 T20055 T20051 appos polydI-dC,EDTA
R15017 T20056 T20055 cc and,polydI-dC
R15018 T20057 T20058 nummod 10,%
R15019 T20058 T20059 compound %,Glycerol
R15020 T20059 T20055 conj Glycerol,polydI-dC
R15021 T20060 T20059 punct ),Glycerol
R15022 T20061 T20051 prep at,EDTA
R15023 T20062 T20063 nummod 30,°
R15024 T20063 T20061 pobj °,at
R15025 T20064 T20051 conj C,EDTA
R15026 T20065 T20047 prep for,DTT
R15027 T20066 T20067 nummod 30,minutes
R15028 T20067 T20065 pobj minutes,for
R15029 T20068 T20031 punct .,incubated
R15030 T20069 T20071 det The,DNA
R15031 T20070 T20071 amod free,DNA
R15032 T20071 T20076 nsubjpass DNA,resolved
R15033 T20072 T20071 cc and,DNA
R15034 T20073 T20074 amod DNA-protein,mixtures
R15035 T20074 T20071 conj mixtures,DNA
R15036 T20075 T20076 auxpass were,resolved
R15037 T20076 T20076 ROOT resolved,resolved
R15038 T20077 T20076 prep in,resolved
R15039 T20078 T20079 nummod 5,%
R15040 T20079 T20082 nmod %,gels
R15041 T20080 T20082 amod native,gels
R15042 T20081 T20082 compound polyacrylamide,gels
R15043 T20082 T20077 pobj gels,in
R15044 T20083 T20076 prep in,resolved
R15045 T20084 T20087 nummod 0.5,buffer
R15046 T20085 T20087 nummod ×,buffer
R15047 T20086 T20087 compound TBE,buffer
R15048 T20087 T20083 pobj buffer,in
R15049 T20088 T20091 punct (,Tris
R15050 T20089 T20091 nummod 45,Tris
R15051 T20090 T20091 compound mM,Tris
R15052 T20091 T20087 appos Tris,buffer
R15053 T20092 T20091 punct ",",Tris
R15054 T20093 T20094 nummod 45,mM
R15055 T20094 T20096 nmod mM,acid
R15056 T20095 T20096 compound boric,acid
R15057 T20096 T20091 appos acid,Tris
R15058 T20097 T20096 cc and,acid
R15059 T20098 T20100 nummod 1,EDTA
R15060 T20099 T20100 compound mM,EDTA
R15061 T20100 T20096 conj EDTA,acid
R15062 T20101 T20100 punct ",",EDTA
R15063 T20102 T20100 appos pH,EDTA
R15064 T20103 T20102 nummod 8.3,pH
R15065 T20104 T20100 punct ),EDTA
R15066 T20105 T20076 agent by,resolved
R15067 T20106 T20105 pobj electrophoresis,by
R15068 T20107 T20076 punct .,resolved
R15069 T20108 T20109 det The,gel
R15070 T20109 T20111 nsubjpass gel,dried
R15071 T20110 T20111 auxpass was,dried
R15072 T20111 T20111 ROOT dried,dried
R15073 T20112 T20111 cc and,dried
R15074 T20113 T20111 conj subjected,dried
R15075 T20114 T20115 aux to,autoradiography
R15076 T20115 T20113 xcomp autoradiography,subjected
R15077 T20116 T20115 dobj analysis,autoradiography
R15078 T20117 T20111 punct .,dried