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PMC:3245220 / 14033-15167 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T23953 255-260 P09603 denotes M-CSF
T23954 257-260 P04141 denotes CSF
T23955 449-458 P42574 denotes caspase-3
T23956 490-497 P60709 denotes β-actin
T23957 552-561 P42574 denotes caspase-3
T23958 589-596 P60709 denotes β-actin
T23959 778-787 P08758 denotes Annexin V
T23960 895-904 P08758 denotes Annexin V
T23961 953-958 P09603 denotes M-CSF
T23962 955-958 P04141 denotes CSF
T23963 1089-1094 P09603 denotes M-CSF
T23964 1091-1094 P04141 denotes CSF
T23965 1115-1120 P09603 denotes M-CSF
T23966 1117-1120 P04141 denotes CSF

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T23967 545-551 Positive_regulation denotes active
T23968 908-916 Negative_regulation denotes negative
T23969 490-497 Protein denotes β-actin
T23970 552-561 Protein denotes caspase-3
T23971 589-596 Protein denotes β-actin
T23972 778-787 Protein denotes Annexin V
T23973 895-904 Protein denotes Annexin V
T23974 255-260 Protein denotes M-CSF
T23975 953-958 Protein denotes M-CSF
T23976 1089-1094 Protein denotes M-CSF
T23977 1115-1120 Protein denotes M-CSF
R18343 T23970 T23967 themeOf caspase-3,active
R18344 T23973 T23968 themeOf Annexin V,negative

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T24061 58-61 Protein denotes PKC
T24062 65-70 Protein denotes NF-κB
T24063 44-54 Negative_regulation denotes Inhibition
T24064 44-54 Negative_regulation denotes Inhibition
T24065 255-260 Protein denotes M-CSF
T24066 449-458 Protein denotes caspase-3
T24067 490-497 Protein denotes β-actin
T24068 552-561 Protein denotes caspase-3
T24069 589-596 Protein denotes β-actin
T24070 597-604 Regulation denotes control
T24071 1089-1094 Protein denotes M-CSF
R18355 T24061 T24063 themeOf PKC,Inhibition
R18356 T24062 T24064 themeOf NF-κB,Inhibition
R18357 T24069 T24070 themeOf β-actin,control

events-check-again

Id Subject Object Predicate Lexical cue
T24050 255-260 Protein denotes M-CSF
T24051 490-497 Protein denotes β-actin
T24052 545-551 Positive_regulation denotes active
T24053 552-561 Protein denotes caspase-3
T24054 589-596 Protein denotes β-actin
T24055 778-787 Protein denotes Annexin V
T24056 895-904 Protein denotes Annexin V
T24057 908-916 Negative_regulation denotes negative
T24058 953-958 Protein denotes M-CSF
T24059 1089-1094 Protein denotes M-CSF
T24060 1115-1120 Protein denotes M-CSF
R18353 T24053 T24052 themeOf caspase-3,active
R18354 T24056 T24057 themeOf Annexin V,negative

GO-CC

Id Subject Object Predicate Lexical cue
T23992 286-291 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T23993 888-893 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T23994 926-931 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T23995 401-409 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T23996 401-409 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

GO-MF

Id Subject Object Predicate Lexical cue
T23990 58-61 http://purl.obolibrary.org/obo/GO_0004697 denotes PKC
T23991 401-409 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-BP

Id Subject Object Predicate Lexical cue
T24072 79-88 http://purl.obolibrary.org/obo/GO_0006915 denotes apoptosis
T24073 727-736 http://purl.obolibrary.org/obo/GO_0006915 denotes Apoptosis
T24074 79-88 http://purl.obolibrary.org/obo/GO_0097194 denotes apoptosis
T24075 727-736 http://purl.obolibrary.org/obo/GO_0097194 denotes Apoptosis

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T23763 44-54 NN denotes Inhibition
T23764 55-57 IN denotes of
T23765 58-61 NN denotes PKC
T23766 62-64 CC denotes or
T23767 65-70 NN denotes NF-κB
T23768 71-78 VB denotes induces
T23769 79-88 NN denotes apoptosis
T23770 89-91 IN denotes in
T23771 92-96 NN denotes MDMs
T23772 98-99 -LRB- denotes (
T23773 99-100 NN denotes A
T23774 100-101 -RRB- denotes )
T23775 102-106 NN denotes MDMs
T23776 107-111 VB denotes were
T23777 112-125 JJ denotes pre-incubated
T23778 126-128 IN denotes in
T23779 129-133 NN denotes RPMI
T23780 134-140 NN denotes medium
T23781 141-151 VB denotes containing
T23782 152-162 NN denotes inhibitors
T23783 163-164 -LRB- denotes (
T23784 164-174 NN denotes Ro-31-8220
T23785 174-175 -COLON- denotes :
T23786 176-177 CD denotes 5
T23787 178-180 NN denotes µM
T23788 180-181 -COMMA- denotes ,
T23789 182-189 NN denotes Gö-6976
T23790 189-190 -COLON- denotes :
T23791 191-192 CD denotes 5
T23792 193-195 NN denotes µM
T23793 195-196 -COMMA- denotes ,
T23794 197-205 NNP denotes BAPTA/AM
T23795 205-206 -COLON- denotes :
T23796 207-210 CD denotes 2.5
T23797 211-213 NN denotes µM
T23798 213-214 -RRB- denotes )
T23799 215-218 IN denotes for
T23800 219-221 CD denotes 30
T23801 222-229 NN denotes minutes
T23802 230-235 RB denotes prior
T23803 236-238 TO denotes to
T23804 239-242 DT denotes the
T23805 243-251 NN denotes addition
T23806 252-254 IN denotes of
T23807 255-260 NN denotes M-CSF
T23808 262-264 IN denotes As
T23809 265-266 DT denotes a
T23810 267-274 NN denotes control
T23811 274-275 -COMMA- denotes ,
T23812 276-285 JJ denotes untreated
T23813 286-291 NN denotes cells
T23814 292-296 VB denotes were
T23815 297-306 VB denotes incubated
T23816 307-311 IN denotes with
T23817 312-320 NN denotes dimethyl
T23818 321-330 NN denotes sulfoxide
T23819 331-332 -LRB- denotes (
T23820 332-336 NN denotes DMSO
T23821 336-337 -RRB- denotes )
T23822 339-343 NN denotes Cell
T23823 344-351 NN denotes lysates
T23824 352-356 VB denotes were
T23825 357-365 VB denotes resolved
T23826 366-368 IN denotes by
T23827 369-377 NN denotes SDS-PAGE
T23828 378-381 CC denotes and
T23829 382-395 VB denotes immunoblotted
T23830 396-400 IN denotes with
T23831 401-409 NN denotes antibody
T23832 410-421 VB denotes recognizing
T23833 422-425 DT denotes the
T23834 426-432 JJ denotes active
T23835 433-440 JJ denotes cleaved
T23836 441-445 NN denotes form
T23837 446-448 IN denotes of
T23838 449-458 NN denotes caspase-3
T23839 460-463 DT denotes The
T23840 464-469 NN denotes blots
T23841 470-474 VB denotes were
T23842 475-484 VB denotes reblotted
T23843 485-489 IN denotes with
T23844 490-497 NN denotes β-actin
T23845 498-502 WDT denotes that
T23846 503-509 VB denotes served
T23847 510-512 IN denotes as
T23848 513-514 DT denotes a
T23849 515-522 NN denotes loading
T23850 523-530 NN denotes control
T23851 532-535 DT denotes The
T23852 536-541 NN denotes ratio
T23853 542-544 IN denotes of
T23854 545-551 JJ denotes active
T23855 552-561 NN denotes caspase-3
T23856 562-567 NN denotes bands
T23857 568-569 -LRB- denotes (
T23858 569-571 CD denotes 17
T23859 572-574 NN denotes kD
T23860 575-578 CC denotes and
T23861 579-581 CD denotes 19
T23862 582-584 NN denotes kD
T23863 584-585 -RRB- denotes )
T23864 586-588 TO denotes to
T23865 589-596 NN denotes β-actin
T23866 597-604 NN denotes control
T23867 605-608 VB denotes was
T23868 609-619 VB denotes determined
T23869 620-622 IN denotes by
T23870 623-635 NN denotes densitometry
T23871 636-644 NN denotes analysis
T23872 645-646 -LRB- denotes (
T23873 646-652 NN denotes bottom
T23874 653-658 NN denotes panel
T23875 658-659 -RRB- denotes )
T23876 661-665 NN denotes Data
T23877 666-676 VB denotes represents
T23878 677-680 DT denotes the
T23879 681-685 JJ denotes mean
T23880 686-687 JJ denotes ±
T23881 688-693 NN denotes S.E.M
T23882 694-698 IN denotes from
T23883 699-702 CD denotes two
T23884 703-714 JJ denotes independent
T23885 715-722 NN denotes donors.
T23886 723-724 -LRB- denotes (
T23887 724-725 NN denotes B
T23888 725-726 -RRB- denotes )
T23889 727-736 NN denotes Apoptosis
T23890 737-739 IN denotes of
T23891 740-743 DT denotes the
T23892 744-751 VB denotes treated
T23893 752-756 NN denotes MDMs
T23894 757-760 VB denotes was
T23895 761-765 RB denotes also
T23896 766-774 VB denotes measured
T23897 775-777 IN denotes by
T23898 778-785 NN denotes Annexin
T23899 786-792 NN denotes V-FITC
T23900 793-796 CC denotes and
T23901 797-806 NN denotes propidium
T23902 807-813 NN denotes iodine
T23903 814-815 -LRB- denotes (
T23904 815-817 NN denotes PI
T23905 817-818 -RRB- denotes )
T23906 819-827 NN denotes staining
T23907 828-831 CC denotes and
T23908 832-840 VB denotes analyzed
T23909 841-843 IN denotes by
T23910 844-848 NN denotes flow
T23911 849-858 NN denotes cytometry
T23912 860-863 DT denotes The
T23913 864-874 NN denotes percentage
T23914 875-877 IN denotes of
T23915 878-887 VB denotes surviving
T23916 888-893 NN denotes cells
T23917 894-895 -LRB- denotes (
T23918 895-902 NN denotes Annexin
T23919 903-907 NN denotes V/PI
T23920 908-916 JJ denotes negative
T23921 916-917 -RRB- denotes )
T23922 918-921 IN denotes for
T23923 922-925 DT denotes the
T23924 926-931 NN denotes cells
T23925 932-939 VB denotes treated
T23926 940-944 IN denotes with
T23927 945-958 NN denotes vehicle/M-CSF
T23928 959-962 VB denotes was
T23929 963-974 RB denotes arbitrarily
T23930 975-978 VB denotes set
T23931 979-981 IN denotes as
T23932 982-985 CD denotes 100
T23933 987-991 NN denotes Data
T23934 992-997 VB denotes shown
T23935 998-1007 VB denotes represent
T23936 1008-1011 DT denotes the
T23937 1012-1016 JJ denotes mean
T23938 1017-1018 JJ denotes ±
T23939 1019-1024 NN denotes S.E.M
T23940 1025-1029 IN denotes from
T23941 1030-1035 CD denotes three
T23942 1036-1047 JJ denotes independent
T23943 1048-1060 NN denotes experiments.
T23944 1061-1065 NNP denotes *The
T23945 1066-1074 NN denotes p-values
T23946 1075-1077 IN denotes of
T23947 1078-1094 NN denotes inhibitors/M-CSF
T23948 1095-1103 VB denotes compared
T23949 1104-1106 TO denotes to
T23950 1107-1120 NN denotes vehicle/M-CSF
T23951 1121-1125 VB denotes were
T23952 1126-1131 CD denotes ≤0.05
R18154 T23763 T23764 arg1Of Inhibition,of
R18155 T23763 T23768 arg1Of Inhibition,induces
R18156 T23765 T23766 arg1Of PKC,or
R18157 T23766 T23764 arg2Of or,of
R18158 T23767 T23766 arg2Of NF-κB,or
R18159 T23768 T23770 arg1Of induces,in
R18160 T23769 T23768 arg2Of apoptosis,induces
R18161 T23771 T23770 arg2Of MDMs,in
R18162 T23773 T23772 arg2Of A,(
R18163 T23774 T23772 arg3Of ),(
R18164 T23775 T23772 arg1Of MDMs,(
R18165 T23775 T23776 arg1Of MDMs,were
R18166 T23775 T23777 arg1Of MDMs,pre-incubated
R18167 T23776 T23778 arg1Of were,in
R18168 T23777 T23776 arg2Of pre-incubated,were
R18169 T23780 T23778 arg2Of medium,in
R18170 T23780 T23779 arg1Of medium,RPMI
R18171 T23780 T23781 arg1Of medium,containing
R18172 T23782 T23781 arg2Of inhibitors,containing
R18173 T23782 T23783 arg1Of inhibitors,(
R18174 T23784 T23783 arg2Of Ro-31-8220,(
R18175 T23785 T23783 arg3Of :,(
R18176 T23787 T23786 arg1Of µM,5
R18177 T23787 T23788 arg1Of µM,","
R18178 T23789 T23788 arg2Of Gö-6976,","
R18179 T23789 T23790 arg1Of Gö-6976,:
R18180 T23792 T23791 arg1Of µM,5
R18181 T23792 T23793 arg1Of µM,","
R18182 T23794 T23793 arg2Of BAPTA/AM,","
R18183 T23794 T23795 arg1Of BAPTA/AM,:
R18184 T23797 T23796 arg1Of µM,2.5
R18185 T23797 T23798 arg1Of µM,)
R18186 T23797 T23799 arg1Of µM,for
R18187 T23801 T23799 arg2Of minutes,for
R18188 T23801 T23800 arg1Of minutes,30
R18189 T23801 T23803 arg1Of minutes,to
R18190 T23803 T23802 arg1Of to,prior
R18191 T23805 T23803 arg2Of addition,to
R18192 T23805 T23804 arg1Of addition,the
R18193 T23805 T23806 arg1Of addition,of
R18194 T23807 T23806 arg2Of M-CSF,of
R18195 T23810 T23808 arg2Of control,As
R18196 T23810 T23809 arg1Of control,a
R18197 T23813 T23812 arg1Of cells,untreated
R18198 T23813 T23814 arg1Of cells,were
R18199 T23813 T23815 arg2Of cells,incubated
R18200 T23815 T23808 arg1Of incubated,As
R18201 T23815 T23811 arg1Of incubated,","
R18202 T23815 T23814 arg2Of incubated,were
R18203 T23815 T23816 arg1Of incubated,with
R18204 T23818 T23816 arg2Of sulfoxide,with
R18205 T23818 T23817 arg1Of sulfoxide,dimethyl
R18206 T23818 T23819 arg1Of sulfoxide,(
R18207 T23820 T23819 arg2Of DMSO,(
R18208 T23821 T23819 arg3Of ),(
R18209 T23823 T23822 arg1Of lysates,Cell
R18210 T23823 T23824 arg1Of lysates,were
R18211 T23823 T23825 arg2Of lysates,resolved
R18212 T23823 T23829 arg2Of lysates,immunoblotted
R18213 T23825 T23826 arg1Of resolved,by
R18214 T23825 T23828 arg1Of resolved,and
R18215 T23827 T23826 arg2Of SDS-PAGE,by
R18216 T23828 T23824 arg2Of and,were
R18217 T23829 T23828 arg2Of immunoblotted,and
R18218 T23829 T23830 arg1Of immunoblotted,with
R18219 T23831 T23830 arg2Of antibody,with
R18220 T23831 T23832 arg1Of antibody,recognizing
R18221 T23832 T23830 arg3Of recognizing,with
R18222 T23836 T23832 arg2Of form,recognizing
R18223 T23836 T23833 arg1Of form,the
R18224 T23836 T23834 arg1Of form,active
R18225 T23836 T23835 arg1Of form,cleaved
R18226 T23836 T23837 arg1Of form,of
R18227 T23838 T23837 arg2Of caspase-3,of
R18228 T23840 T23839 arg1Of blots,The
R18229 T23840 T23841 arg1Of blots,were
R18230 T23840 T23842 arg2Of blots,reblotted
R18231 T23842 T23841 arg2Of reblotted,were
R18232 T23842 T23843 arg1Of reblotted,with
R18233 T23844 T23843 arg2Of β-actin,with
R18234 T23844 T23845 arg1Of β-actin,that
R18235 T23844 T23846 arg1Of β-actin,served
R18236 T23846 T23847 arg1Of served,as
R18237 T23850 T23847 arg2Of control,as
R18238 T23850 T23848 arg1Of control,a
R18239 T23850 T23849 arg1Of control,loading
R18240 T23852 T23851 arg1Of ratio,The
R18241 T23852 T23853 arg1Of ratio,of
R18242 T23852 T23864 arg1Of ratio,to
R18243 T23852 T23867 arg1Of ratio,was
R18244 T23852 T23868 arg2Of ratio,determined
R18245 T23856 T23853 arg2Of bands,of
R18246 T23856 T23854 arg1Of bands,active
R18247 T23856 T23855 arg1Of bands,caspase-3
R18248 T23856 T23857 arg1Of bands,(
R18249 T23859 T23858 arg1Of kD,17
R18250 T23859 T23860 arg1Of kD,and
R18251 T23860 T23857 arg2Of and,(
R18252 T23862 T23860 arg2Of kD,and
R18253 T23862 T23861 arg1Of kD,19
R18254 T23863 T23857 arg3Of ),(
R18255 T23866 T23864 arg2Of control,to
R18256 T23866 T23865 arg1Of control,β-actin
R18257 T23868 T23867 arg2Of determined,was
R18258 T23871 T23868 arg1Of analysis,determined
R18259 T23871 T23869 arg2Of analysis,by
R18260 T23871 T23870 arg1Of analysis,densitometry
R18261 T23871 T23872 arg1Of analysis,(
R18262 T23874 T23872 arg2Of panel,(
R18263 T23874 T23873 arg1Of panel,bottom
R18264 T23875 T23872 arg3Of ),(
R18265 T23876 T23877 arg1Of Data,represents
R18266 T23881 T23877 arg2Of S.E.M,represents
R18267 T23881 T23878 arg1Of S.E.M,the
R18268 T23881 T23879 arg1Of S.E.M,mean
R18269 T23881 T23880 arg1Of S.E.M,±
R18270 T23881 T23882 arg1Of S.E.M,from
R18271 T23881 T23894 modOf S.E.M,was
R18272 T23885 T23882 arg2Of donors.,from
R18273 T23885 T23883 arg1Of donors.,two
R18274 T23885 T23884 arg1Of donors.,independent
R18275 T23885 T23886 arg1Of donors.,(
R18276 T23887 T23886 arg2Of B,(
R18277 T23888 T23886 arg3Of ),(
R18278 T23889 T23890 arg1Of Apoptosis,of
R18279 T23889 T23894 arg1Of Apoptosis,was
R18280 T23889 T23896 arg2Of Apoptosis,measured
R18281 T23889 T23908 arg2Of Apoptosis,analyzed
R18282 T23893 T23890 arg2Of MDMs,of
R18283 T23893 T23891 arg1Of MDMs,the
R18284 T23893 T23892 arg2Of MDMs,treated
R18285 T23896 T23907 arg1Of measured,and
R18286 T23899 T23898 arg1Of V-FITC,Annexin
R18287 T23899 T23900 arg1Of V-FITC,and
R18288 T23900 T23896 arg1Of and,measured
R18289 T23900 T23897 arg2Of and,by
R18290 T23902 T23901 arg1Of iodine,propidium
R18291 T23902 T23903 arg1Of iodine,(
R18292 T23904 T23903 arg2Of PI,(
R18293 T23905 T23903 arg3Of ),(
R18294 T23906 T23900 arg2Of staining,and
R18295 T23906 T23902 arg1Of staining,iodine
R18296 T23907 T23894 arg2Of and,was
R18297 T23907 T23895 arg1Of and,also
R18298 T23908 T23907 arg2Of analyzed,and
R18299 T23911 T23908 arg1Of cytometry,analyzed
R18300 T23911 T23909 arg2Of cytometry,by
R18301 T23911 T23910 arg1Of cytometry,flow
R18302 T23913 T23912 arg1Of percentage,The
R18303 T23913 T23914 arg1Of percentage,of
R18304 T23913 T23922 arg1Of percentage,for
R18305 T23913 T23928 arg1Of percentage,was
R18306 T23913 T23930 arg2Of percentage,set
R18307 T23916 T23914 arg2Of cells,of
R18308 T23916 T23915 arg1Of cells,surviving
R18309 T23916 T23917 arg1Of cells,(
R18310 T23919 T23917 arg2Of V/PI,(
R18311 T23919 T23918 arg1Of V/PI,Annexin
R18312 T23919 T23920 arg1Of V/PI,negative
R18313 T23921 T23917 arg3Of ),(
R18314 T23924 T23922 arg2Of cells,for
R18315 T23924 T23923 arg1Of cells,the
R18316 T23924 T23925 arg2Of cells,treated
R18317 T23925 T23926 arg1Of treated,with
R18318 T23927 T23926 arg2Of vehicle/M-CSF,with
R18319 T23930 T23928 arg2Of set,was
R18320 T23930 T23929 arg1Of set,arbitrarily
R18321 T23930 T23931 arg1Of set,as
R18322 T23932 T23931 arg2Of 100,as
R18323 T23933 T23934 arg2Of Data,shown
R18324 T23933 T23935 arg1Of Data,represent
R18325 T23939 T23935 arg2Of S.E.M,represent
R18326 T23939 T23936 arg1Of S.E.M,the
R18327 T23939 T23937 arg1Of S.E.M,mean
R18328 T23939 T23938 arg1Of S.E.M,±
R18329 T23939 T23940 arg1Of S.E.M,from
R18330 T23943 T23940 arg2Of experiments.,from
R18331 T23943 T23941 arg1Of experiments.,three
R18332 T23943 T23942 arg1Of experiments.,independent
R18333 T23943 T23951 modOf experiments.,were
R18334 T23945 T23944 arg1Of p-values,*The
R18335 T23945 T23946 arg1Of p-values,of
R18336 T23945 T23951 arg1Of p-values,were
R18337 T23945 T23952 arg1Of p-values,≤0.05
R18338 T23947 T23946 arg2Of inhibitors/M-CSF,of
R18339 T23947 T23948 arg1Of inhibitors/M-CSF,compared
R18340 T23949 T23948 arg2Of to,compared
R18341 T23950 T23949 arg2Of vehicle/M-CSF,to
R18342 T23952 T23951 arg2Of ≤0.05,were

sentences

Id Subject Object Predicate Lexical cue
T23548 44-97 Sentence denotes Inhibition of PKC or NF-κB induces apoptosis in MDMs.
T23549 98-175 Sentence denotes (A) MDMs were pre-incubated in RPMI medium containing inhibitors (Ro-31-8220:
T23550 176-190 Sentence denotes 5 µM, Gö-6976:
T23551 191-206 Sentence denotes 5 µM, BAPTA/AM:
T23552 207-261 Sentence denotes 2.5 µM) for 30 minutes prior to the addition of M-CSF.
T23553 262-338 Sentence denotes As a control, untreated cells were incubated with dimethyl sulfoxide (DMSO).
T23554 339-459 Sentence denotes Cell lysates were resolved by SDS-PAGE and immunoblotted with antibody recognizing the active cleaved form of caspase-3.
T23555 460-531 Sentence denotes The blots were reblotted with β-actin that served as a loading control.
T23556 532-660 Sentence denotes The ratio of active caspase-3 bands (17 kD and 19 kD) to β-actin control was determined by densitometry analysis (bottom panel).
T23557 661-859 Sentence denotes Data represents the mean ± S.E.M from two independent donors. (B) Apoptosis of the treated MDMs was also measured by Annexin V-FITC and propidium iodine (PI) staining and analyzed by flow cytometry.
T23558 860-986 Sentence denotes The percentage of surviving cells (Annexin V/PI negative) for the cells treated with vehicle/M-CSF was arbitrarily set as 100.
T23559 987-1132 Sentence denotes Data shown represent the mean ± S.E.M from three independent experiments. *The p-values of inhibitors/M-CSF compared to vehicle/M-CSF were ≤0.05.
T101 0-97 Sentence denotes 10.1371/journal.pone.0028081.g003 Figure 3 Inhibition of PKC or NF-κB induces apoptosis in MDMs.
T102 98-175 Sentence denotes (A) MDMs were pre-incubated in RPMI medium containing inhibitors (Ro-31-8220:
T103 176-190 Sentence denotes 5 µM, Gö-6976:
T104 191-206 Sentence denotes 5 µM, BAPTA/AM:
T105 207-261 Sentence denotes 2.5 µM) for 30 minutes prior to the addition of M-CSF.
T106 262-338 Sentence denotes As a control, untreated cells were incubated with dimethyl sulfoxide (DMSO).
T107 339-459 Sentence denotes Cell lysates were resolved by SDS-PAGE and immunoblotted with antibody recognizing the active cleaved form of caspase-3.
T108 460-531 Sentence denotes The blots were reblotted with β-actin that served as a loading control.
T109 532-660 Sentence denotes The ratio of active caspase-3 bands (17 kD and 19 kD) to β-actin control was determined by densitometry analysis (bottom panel).
T110 661-859 Sentence denotes Data represents the mean ± S.E.M from two independent donors. (B) Apoptosis of the treated MDMs was also measured by Annexin V-FITC and propidium iodine (PI) staining and analyzed by flow cytometry.
T111 860-986 Sentence denotes The percentage of surviving cells (Annexin V/PI negative) for the cells treated with vehicle/M-CSF was arbitrarily set as 100.
T112 987-1132 Sentence denotes Data shown represent the mean ± S.E.M from three independent experiments. *The p-values of inhibitors/M-CSF compared to vehicle/M-CSF were ≤0.05.

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T23560 44-54 NNP denotes Inhibition
T23561 55-57 IN denotes of
T23562 58-61 NNP denotes PKC
T23563 62-64 CC denotes or
T23564 65-70 NNP denotes NF-κB
T23565 71-78 VBZ denotes induces
T23566 79-88 NN denotes apoptosis
T23567 89-91 IN denotes in
T23568 92-96 NNS denotes MDMs
T23569 96-97 . denotes .
T23570 98-99 -LRB- denotes (
T23571 99-100 NNP denotes A
T23572 100-101 -RRB- denotes )
T23573 102-106 NNS denotes MDMs
T23574 107-111 VBD denotes were
T23575 112-125 VBN denotes pre-incubated
T23576 126-128 IN denotes in
T23577 129-133 NNP denotes RPMI
T23578 134-140 NN denotes medium
T23579 141-151 VBG denotes containing
T23580 152-162 NNS denotes inhibitors
T23581 163-164 -LRB- denotes (
T23582 164-174 NN denotes Ro-31-8220
T23583 174-175 : denotes :
T23584 176-177 CD denotes 5
T23585 178-180 NNP denotes µM
T23586 180-181 , denotes ,
T23587 182-189 NNP denotes Gö-6976
T23588 189-190 : denotes :
T23589 191-192 CD denotes 5
T23590 193-195 NNP denotes µM
T23591 195-196 , denotes ,
T23592 197-205 NNP denotes BAPTA/AM
T23593 205-206 : denotes :
T23594 207-210 CD denotes 2.5
T23595 211-213 NNP denotes µM
T23596 213-214 -RRB- denotes )
T23597 215-218 IN denotes for
T23598 219-221 CD denotes 30
T23599 222-229 NNS denotes minutes
T23600 230-235 RB denotes prior
T23601 236-238 TO denotes to
T23602 239-242 DT denotes the
T23603 243-251 NN denotes addition
T23604 252-254 IN denotes of
T23605 255-260 NNP denotes M-CSF
T23606 260-261 . denotes .
T23607 262-264 IN denotes As
T23608 265-266 DT denotes a
T23609 267-274 NN denotes control
T23610 274-275 , denotes ,
T23611 276-285 JJ denotes untreated
T23612 286-291 NNS denotes cells
T23613 292-296 VBD denotes were
T23614 297-306 VBN denotes incubated
T23615 307-311 IN denotes with
T23616 312-320 NN denotes dimethyl
T23617 321-330 NN denotes sulfoxide
T23618 331-332 -LRB- denotes (
T23619 332-336 NNP denotes DMSO
T23620 336-337 -RRB- denotes )
T23621 337-338 . denotes .
T23622 339-343 NN denotes Cell
T23623 344-351 NNS denotes lysates
T23624 352-356 VBD denotes were
T23625 357-365 VBN denotes resolved
T23626 366-368 IN denotes by
T23627 369-377 NNP denotes SDS-PAGE
T23628 378-381 CC denotes and
T23629 382-395 VBN denotes immunoblotted
T23630 396-400 IN denotes with
T23631 401-409 NN denotes antibody
T23632 410-421 VBG denotes recognizing
T23633 422-425 DT denotes the
T23634 426-432 JJ denotes active
T23635 433-440 JJ denotes cleaved
T23636 441-445 NN denotes form
T23637 446-448 IN denotes of
T23638 449-458 JJ denotes caspase-3
T23639 458-459 . denotes .
T23640 460-463 DT denotes The
T23641 464-469 NNS denotes blots
T23642 470-474 VBD denotes were
T23643 475-484 VBN denotes reblotted
T23644 485-489 IN denotes with
T23645 490-497 NN denotes β-actin
T23646 498-502 IN denotes that
T23647 503-509 VBD denotes served
T23648 510-512 IN denotes as
T23649 513-514 DT denotes a
T23650 515-522 VBG denotes loading
T23651 523-530 NN denotes control
T23652 530-531 . denotes .
T23653 532-535 DT denotes The
T23654 536-541 NN denotes ratio
T23655 542-544 IN denotes of
T23656 545-551 JJ denotes active
T23657 552-561 JJ denotes caspase-3
T23658 562-567 NNS denotes bands
T23659 568-569 -LRB- denotes (
T23660 569-571 CD denotes 17
T23661 572-574 NN denotes kD
T23662 575-578 CC denotes and
T23663 579-581 CD denotes 19
T23664 582-584 NN denotes kD
T23665 584-585 -RRB- denotes )
T23666 586-588 TO denotes to
T23667 589-596 VB denotes β-actin
T23668 597-604 NN denotes control
T23669 605-608 VBD denotes was
T23670 609-619 VBN denotes determined
T23671 620-622 IN denotes by
T23672 623-635 NN denotes densitometry
T23673 636-644 NN denotes analysis
T23674 645-646 -LRB- denotes (
T23675 646-652 JJ denotes bottom
T23676 653-658 NN denotes panel
T23677 658-659 -RRB- denotes )
T23678 659-660 . denotes .
T23679 661-665 NNP denotes Data
T23680 666-676 VBZ denotes represents
T23681 677-680 DT denotes the
T23682 681-685 JJ denotes mean
T23683 686-687 NN denotes ±
T23684 688-693 NNP denotes S.E.M
T23685 694-698 IN denotes from
T23686 699-702 CD denotes two
T23687 703-714 JJ denotes independent
T23688 715-721 NNS denotes donors
T23689 721-722 . denotes .
T23690 723-724 -LRB- denotes (
T23691 724-725 NNP denotes B
T23692 725-726 -RRB- denotes )
T23693 727-736 NNP denotes Apoptosis
T23694 737-739 IN denotes of
T23695 740-743 DT denotes the
T23696 744-751 VBN denotes treated
T23697 752-756 NNS denotes MDMs
T23698 757-760 VBD denotes was
T23699 761-765 RB denotes also
T23700 766-774 VBN denotes measured
T23701 775-777 IN denotes by
T23702 778-785 NNP denotes Annexin
T23703 786-792 NNP denotes V-FITC
T23704 793-796 CC denotes and
T23705 797-806 NN denotes propidium
T23706 807-813 NN denotes iodine
T23707 814-815 -LRB- denotes (
T23708 815-817 NNP denotes PI
T23709 817-818 -RRB- denotes )
T23710 819-827 VBG denotes staining
T23711 828-831 CC denotes and
T23712 832-840 VBN denotes analyzed
T23713 841-843 IN denotes by
T23714 844-848 NN denotes flow
T23715 849-858 NN denotes cytometry
T23716 858-859 . denotes .
T23717 860-863 DT denotes The
T23718 864-874 NN denotes percentage
T23719 875-877 IN denotes of
T23720 878-887 VBG denotes surviving
T23721 888-893 NNS denotes cells
T23722 894-895 -LRB- denotes (
T23723 895-902 NNP denotes Annexin
T23724 903-907 NNP denotes V/PI
T23725 908-916 JJ denotes negative
T23726 916-917 -RRB- denotes )
T23727 918-921 IN denotes for
T23728 922-925 DT denotes the
T23729 926-931 NNS denotes cells
T23730 932-939 VBN denotes treated
T23731 940-944 IN denotes with
T23732 945-958 NNP denotes vehicle/M-CSF
T23733 959-962 VBD denotes was
T23734 963-974 RB denotes arbitrarily
T23735 975-978 VBN denotes set
T23736 979-981 IN denotes as
T23737 982-985 CD denotes 100
T23738 985-986 . denotes .
T23739 987-991 NNP denotes Data
T23740 992-997 VBN denotes shown
T23741 998-1007 VBP denotes represent
T23742 1008-1011 DT denotes the
T23743 1012-1016 JJ denotes mean
T23744 1017-1018 NN denotes ±
T23745 1019-1024 NNP denotes S.E.M
T23746 1025-1029 IN denotes from
T23747 1030-1035 CD denotes three
T23748 1036-1047 JJ denotes independent
T23749 1048-1059 NNS denotes experiments
T23750 1059-1060 . denotes .
T23751 1061-1062 SYM denotes *
T23752 1062-1065 DT denotes The
T23753 1066-1074 NNS denotes p-values
T23754 1075-1077 IN denotes of
T23755 1078-1094 JJ denotes inhibitors/M-CSF
T23756 1095-1103 VBN denotes compared
T23757 1104-1106 TO denotes to
T23758 1107-1120 NNP denotes vehicle/M-CSF
T23759 1121-1125 VBD denotes were
T23760 1126-1127 CD denotes ≤
T23761 1127-1131 CD denotes 0.05
T23762 1131-1132 . denotes .
R17951 T23560 T23565 nsubj Inhibition,induces
R17952 T23561 T23560 prep of,Inhibition
R17953 T23562 T23561 pobj PKC,of
R17954 T23563 T23562 cc or,PKC
R17955 T23564 T23562 conj NF-κB,PKC
R17956 T23565 T23565 ROOT induces,induces
R17957 T23566 T23565 dobj apoptosis,induces
R17958 T23567 T23565 prep in,induces
R17959 T23568 T23567 pobj MDMs,in
R17960 T23569 T23565 punct .,induces
R17961 T23570 T23575 punct (,pre-incubated
R17962 T23571 T23573 nmod A,MDMs
R17963 T23572 T23573 punct ),MDMs
R17964 T23573 T23575 nsubjpass MDMs,pre-incubated
R17965 T23574 T23575 auxpass were,pre-incubated
R17966 T23575 T23575 ROOT pre-incubated,pre-incubated
R17967 T23576 T23575 prep in,pre-incubated
R17968 T23577 T23578 compound RPMI,medium
R17969 T23578 T23576 pobj medium,in
R17970 T23579 T23578 acl containing,medium
R17971 T23580 T23579 dobj inhibitors,containing
R17972 T23581 T23580 punct (,inhibitors
R17973 T23582 T23580 appos Ro-31-8220,inhibitors
R17974 T23583 T23582 punct :,Ro-31-8220
R17975 T23584 T23585 nummod 5,µM
R17976 T23585 T23582 appos µM,Ro-31-8220
R17977 T23586 T23585 punct ",",µM
R17978 T23587 T23585 appos Gö-6976,µM
R17979 T23588 T23587 punct :,Gö-6976
R17980 T23589 T23590 nummod 5,µM
R17981 T23590 T23587 conj µM,Gö-6976
R17982 T23591 T23590 punct ",",µM
R17983 T23592 T23590 conj BAPTA/AM,µM
R17984 T23593 T23592 punct :,BAPTA/AM
R17985 T23594 T23595 nummod 2.5,µM
R17986 T23595 T23592 appos µM,BAPTA/AM
R17987 T23596 T23592 punct ),BAPTA/AM
R17988 T23597 T23592 prep for,BAPTA/AM
R17989 T23598 T23599 nummod 30,minutes
R17990 T23599 T23597 pobj minutes,for
R17991 T23600 T23587 npadvmod prior,Gö-6976
R17992 T23601 T23600 prep to,prior
R17993 T23602 T23603 det the,addition
R17994 T23603 T23601 pobj addition,to
R17995 T23604 T23603 prep of,addition
R17996 T23605 T23604 pobj M-CSF,of
R17997 T23606 T23575 punct .,pre-incubated
R17998 T23607 T23614 prep As,incubated
R17999 T23608 T23609 det a,control
R18000 T23609 T23607 pobj control,As
R18001 T23610 T23614 punct ",",incubated
R18002 T23611 T23612 amod untreated,cells
R18003 T23612 T23614 nsubjpass cells,incubated
R18004 T23613 T23614 auxpass were,incubated
R18005 T23614 T23614 ROOT incubated,incubated
R18006 T23615 T23614 prep with,incubated
R18007 T23616 T23617 compound dimethyl,sulfoxide
R18008 T23617 T23615 pobj sulfoxide,with
R18009 T23618 T23617 punct (,sulfoxide
R18010 T23619 T23617 appos DMSO,sulfoxide
R18011 T23620 T23617 punct ),sulfoxide
R18012 T23621 T23614 punct .,incubated
R18013 T23622 T23623 compound Cell,lysates
R18014 T23623 T23625 nsubjpass lysates,resolved
R18015 T23624 T23625 auxpass were,resolved
R18016 T23625 T23625 ROOT resolved,resolved
R18017 T23626 T23625 agent by,resolved
R18018 T23627 T23626 pobj SDS-PAGE,by
R18019 T23628 T23625 cc and,resolved
R18020 T23629 T23625 conj immunoblotted,resolved
R18021 T23630 T23629 prep with,immunoblotted
R18022 T23631 T23630 pobj antibody,with
R18023 T23632 T23630 pcomp recognizing,with
R18024 T23633 T23636 det the,form
R18025 T23634 T23636 amod active,form
R18026 T23635 T23636 amod cleaved,form
R18027 T23636 T23632 dobj form,recognizing
R18028 T23637 T23636 prep of,form
R18029 T23638 T23637 pobj caspase-3,of
R18030 T23639 T23625 punct .,resolved
R18031 T23640 T23641 det The,blots
R18032 T23641 T23643 nsubjpass blots,reblotted
R18033 T23642 T23643 auxpass were,reblotted
R18034 T23643 T23643 ROOT reblotted,reblotted
R18035 T23644 T23643 prep with,reblotted
R18036 T23645 T23644 pobj β-actin,with
R18037 T23646 T23647 nsubj that,served
R18038 T23647 T23643 conj served,reblotted
R18039 T23648 T23647 prep as,served
R18040 T23649 T23651 det a,control
R18041 T23650 T23651 compound loading,control
R18042 T23651 T23648 pobj control,as
R18043 T23652 T23643 punct .,reblotted
R18044 T23653 T23654 det The,ratio
R18045 T23654 T23670 nsubjpass ratio,determined
R18046 T23655 T23654 prep of,ratio
R18047 T23656 T23658 amod active,bands
R18048 T23657 T23658 compound caspase-3,bands
R18049 T23658 T23655 pobj bands,of
R18050 T23659 T23658 punct (,bands
R18051 T23660 T23661 nummod 17,kD
R18052 T23661 T23658 appos kD,bands
R18053 T23662 T23661 cc and,kD
R18054 T23663 T23664 nummod 19,kD
R18055 T23664 T23661 conj kD,kD
R18056 T23665 T23664 punct ),kD
R18057 T23666 T23670 prep to,determined
R18058 T23667 T23668 compound β-actin,control
R18059 T23668 T23666 pobj control,to
R18060 T23669 T23670 auxpass was,determined
R18061 T23670 T23670 ROOT determined,determined
R18062 T23671 T23670 agent by,determined
R18063 T23672 T23673 amod densitometry,analysis
R18064 T23673 T23671 pobj analysis,by
R18065 T23674 T23676 punct (,panel
R18066 T23675 T23676 amod bottom,panel
R18067 T23676 T23673 appos panel,analysis
R18068 T23677 T23670 punct ),determined
R18069 T23678 T23670 punct .,determined
R18070 T23679 T23680 nsubj Data,represents
R18071 T23680 T23680 ROOT represents,represents
R18072 T23681 T23684 det the,S.E.M
R18073 T23682 T23684 amod mean,S.E.M
R18074 T23683 T23684 compound ±,S.E.M
R18075 T23684 T23680 dobj S.E.M,represents
R18076 T23685 T23684 prep from,S.E.M
R18077 T23686 T23688 nummod two,donors
R18078 T23687 T23688 amod independent,donors
R18079 T23688 T23685 pobj donors,from
R18080 T23689 T23680 punct .,represents
R18081 T23690 T23693 punct (,Apoptosis
R18082 T23691 T23693 nmod B,Apoptosis
R18083 T23692 T23693 punct ),Apoptosis
R18084 T23693 T23700 nsubjpass Apoptosis,measured
R18085 T23694 T23693 prep of,Apoptosis
R18086 T23695 T23697 det the,MDMs
R18087 T23696 T23697 amod treated,MDMs
R18088 T23697 T23694 pobj MDMs,of
R18089 T23698 T23700 auxpass was,measured
R18090 T23699 T23700 advmod also,measured
R18091 T23700 T23700 ROOT measured,measured
R18092 T23701 T23700 agent by,measured
R18093 T23702 T23703 compound Annexin,V-FITC
R18094 T23703 T23701 pobj V-FITC,by
R18095 T23704 T23703 cc and,V-FITC
R18096 T23705 T23706 compound propidium,iodine
R18097 T23706 T23703 conj iodine,V-FITC
R18098 T23707 T23708 punct (,PI
R18099 T23708 T23706 appos PI,iodine
R18100 T23709 T23706 punct ),iodine
R18101 T23710 T23700 advcl staining,measured
R18102 T23711 T23700 cc and,measured
R18103 T23712 T23700 conj analyzed,measured
R18104 T23713 T23712 agent by,analyzed
R18105 T23714 T23715 compound flow,cytometry
R18106 T23715 T23713 pobj cytometry,by
R18107 T23716 T23700 punct .,measured
R18108 T23717 T23718 det The,percentage
R18109 T23718 T23735 nsubjpass percentage,set
R18110 T23719 T23718 prep of,percentage
R18111 T23720 T23721 amod surviving,cells
R18112 T23721 T23719 pobj cells,of
R18113 T23722 T23718 punct (,percentage
R18114 T23723 T23724 compound Annexin,V/PI
R18115 T23724 T23718 appos V/PI,percentage
R18116 T23725 T23724 amod negative,V/PI
R18117 T23726 T23718 punct ),percentage
R18118 T23727 T23718 prep for,percentage
R18119 T23728 T23729 det the,cells
R18120 T23729 T23727 pobj cells,for
R18121 T23730 T23729 acl treated,cells
R18122 T23731 T23730 prep with,treated
R18123 T23732 T23731 pobj vehicle/M-CSF,with
R18124 T23733 T23735 auxpass was,set
R18125 T23734 T23735 advmod arbitrarily,set
R18126 T23735 T23735 ROOT set,set
R18127 T23736 T23735 prep as,set
R18128 T23737 T23736 pobj 100,as
R18129 T23738 T23735 punct .,set
R18130 T23739 T23741 nsubj Data,represent
R18131 T23740 T23739 acl shown,Data
R18132 T23741 T23741 ROOT represent,represent
R18133 T23742 T23745 det the,S.E.M
R18134 T23743 T23745 amod mean,S.E.M
R18135 T23744 T23745 compound ±,S.E.M
R18136 T23745 T23741 dobj S.E.M,represent
R18137 T23746 T23741 prep from,represent
R18138 T23747 T23749 nummod three,experiments
R18139 T23748 T23749 amod independent,experiments
R18140 T23749 T23746 pobj experiments,from
R18141 T23750 T23741 punct .,represent
R18142 T23751 T23751 ROOT *,*
R18143 T23752 T23753 det The,p-values
R18144 T23753 T23756 nsubj p-values,compared
R18145 T23754 T23753 prep of,p-values
R18146 T23755 T23754 pobj inhibitors/M-CSF,of
R18147 T23756 T23756 ROOT compared,compared
R18148 T23757 T23756 prep to,compared
R18149 T23758 T23757 pobj vehicle/M-CSF,to
R18150 T23759 T23756 auxpass were,compared
R18151 T23760 T23761 compound ≤,0.05
R18152 T23761 T23759 attr 0.05,were
R18153 T23762 T23756 punct .,compared

test2

Id Subject Object Predicate Lexical cue
T23538 255-260 Protein denotes M-CSF
T23539 490-497 Protein denotes β-actin
T23540 552-561 Protein denotes caspase-3
T23541 589-596 Protein denotes β-actin
T23542 778-787 Protein denotes Annexin V
T23543 895-904 Protein denotes Annexin V
T23544 908-916 Negative_regulation denotes negative
T23545 953-958 Protein denotes M-CSF
T23546 1089-1094 Protein denotes M-CSF
T23547 1115-1120 Protein denotes M-CSF
R17950 T23543 T23544 themeOf Annexin V,negative