PMC:3216505 / 17518-18157 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/3216505","sourcedb":"PMC","sourceid":"3216505","source_url":"https://www.ncbi.nlm.nih.gov/pmc/3216505","text":"Lipids were combined in chloroform:methanol (2∶1, volume ratio) at 10 mg/ml total concentration and dried as a thin layer on indium-tin-oxide coated glass slides (Delta Technologies, Stillwater, MN). The slides were assembled to form a chamber by using 3 mm thick silicon spacers and holding the slides together with office clips. The chamber was filled with sucrose solution (540 mOsmol/kg) and electroformation was performed overnight using an approximately 3 Volt, 10 Hz sinusoidal voltage and copper conductive tape (3M, SPI supplies) for connections. The GUVs were harvested by sedimentation in an iso-osmolar glucose solution at 4°C.","tracks":[]}