PMC:3148254 / 31544-32168 JSONTXT

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{"project":"sentences","denotations":[{"id":"T13563","span":{"begin":0,"end":20},"obj":"Sentence"},{"id":"T13564","span":{"begin":21,"end":114},"obj":"Sentence"},{"id":"T13565","span":{"begin":115,"end":282},"obj":"Sentence"},{"id":"T13566","span":{"begin":283,"end":385},"obj":"Sentence"},{"id":"T13567","span":{"begin":386,"end":548},"obj":"Sentence"},{"id":"T13568","span":{"begin":549,"end":624},"obj":"Sentence"},{"id":"T216","span":{"begin":0,"end":20},"obj":"Sentence"},{"id":"T217","span":{"begin":21,"end":114},"obj":"Sentence"},{"id":"T218","span":{"begin":115,"end":282},"obj":"Sentence"},{"id":"T219","span":{"begin":283,"end":385},"obj":"Sentence"},{"id":"T220","span":{"begin":386,"end":548},"obj":"Sentence"},{"id":"T221","span":{"begin":549,"end":624},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"GLε transcript assay\nThe CD40-simulated activation of GLε transcription was evaluated as previously reported [33]. Briefly, 1×106 cells were stimulated overnight with anti-CD40 antibody (10 µg/ml) or an isotype control antibody, with or without 500 U/ml mouse IL-4 (BD Biosciences). RNA was isolated using Trizol (Invitrogen), and reverse-transcribed (Superscript III kit, Invitrogen). Quantitative PCR for GLε and Hprt1 was performed using SYBR GREEN master mix (Applied Biosystems), and an Applied Biosystems 7900HT Fast Real-Time PCR instrument. Expression of GLε in each sample was normalized to the expression of Hprt1."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T13790","span":{"begin":0,"end":3},"obj":"Q28322"},{"id":"T13791","span":{"begin":25,"end":29},"obj":"P25942"},{"id":"T13792","span":{"begin":54,"end":57},"obj":"Q28322"},{"id":"T13793","span":{"begin":172,"end":176},"obj":"P25942"},{"id":"T13794","span":{"begin":260,"end":264},"obj":"P05112"},{"id":"T13795","span":{"begin":407,"end":410},"obj":"Q28322"},{"id":"T13796","span":{"begin":563,"end":566},"obj":"Q28322"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"GLε transcript assay\nThe CD40-simulated activation of GLε transcription was evaluated as previously reported [33]. Briefly, 1×106 cells were stimulated overnight with anti-CD40 antibody (10 µg/ml) or an isotype control antibody, with or without 500 U/ml mouse IL-4 (BD Biosciences). RNA was isolated using Trizol (Invitrogen), and reverse-transcribed (Superscript III kit, Invitrogen). Quantitative PCR for GLε and Hprt1 was performed using SYBR GREEN master mix (Applied Biosystems), and an Applied Biosystems 7900HT Fast Real-Time PCR instrument. Expression of GLε in each sample was normalized to the expression of Hprt1."}

{"project":"test2","denotations":[{"id":"T13538","span":{"begin":0,"end":3},"obj":"Protein"},{"id":"T13539","span":{"begin":25,"end":29},"obj":"Protein"},{"id":"T13540","span":{"begin":40,"end":50},"obj":"Positive_regulation"},{"id":"T13541","span":{"begin":54,"end":57},"obj":"Protein"},{"id":"T13542","span":{"begin":58,"end":71},"obj":"Transcription"},{"id":"T13543","span":{"begin":260,"end":264},"obj":"Protein"},{"id":"T13544","span":{"begin":407,"end":410},"obj":"Protein"},{"id":"T13545","span":{"begin":415,"end":420},"obj":"Protein"},{"id":"T13546","span":{"begin":549,"end":559},"obj":"Gene_expression"},{"id":"T13547","span":{"begin":563,"end":566},"obj":"Protein"},{"id":"T13548","span":{"begin":604,"end":614},"obj":"Gene_expression"},{"id":"T13549","span":{"begin":618,"end":623},"obj":"Protein"}],"relations":[{"id":"R10642","pred":"causeOf","subj":"T13539","obj":"T13540"},{"id":"R10643","pred":"themeOf","subj":"T13541","obj":"T13542"},{"id":"R10644","pred":"themeOf","subj":"T13542","obj":"T13540"},{"id":"R10645","pred":"themeOf","subj":"T13547","obj":"T13546"},{"id":"R10646","pred":"themeOf","subj":"T13549","obj":"T13548"}],"attributes":[{"id":"M116","pred":"Speculation","subj":"T13538","obj":"true"}],"text":"GLε transcript assay\nThe CD40-simulated activation of GLε transcription was evaluated as previously reported [33]. Briefly, 1×106 cells were stimulated overnight with anti-CD40 antibody (10 µg/ml) or an isotype control antibody, with or without 500 U/ml mouse IL-4 (BD Biosciences). RNA was isolated using Trizol (Invitrogen), and reverse-transcribed (Superscript III kit, Invitrogen). Quantitative PCR for GLε and Hprt1 was performed using SYBR GREEN master mix (Applied Biosystems), and an Applied Biosystems 7900HT Fast Real-Time PCR instrument. Expression of GLε in each sample was normalized to the expression of Hprt1."}