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{"project":"craft-ca-core-dev","denotations":[{"id":"T13086","span":{"begin":8,"end":21},"obj":"GO:0097617"},{"id":"T13087","span":{"begin":71,"end":82},"obj":"CHEBI:42098"},{"id":"T13088","span":{"begin":220,"end":227},"obj":"UBERON:0000922"},{"id":"T13089","span":{"begin":236,"end":241},"obj":"GO:0007567"},{"id":"T13090","span":{"begin":296,"end":300},"obj":"NCBITaxon:10088"},{"id":"T13091","span":{"begin":302,"end":309},"obj":"UBERON:0000922"},{"id":"T13092","span":{"begin":379,"end":384},"obj":"GO:0007567"},{"id":"T13093","span":{"begin":437,"end":442},"obj":"PR:000016145"},{"id":"T13094","span":{"begin":479,"end":486},"obj":"SO:0000112"},{"id":"T13095","span":{"begin":548,"end":553},"obj":"SO:0000147"},{"id":"T13096","span":{"begin":572,"end":575},"obj":"PR:000007070"},{"id":"T13097","span":{"begin":608,"end":615},"obj":"SO:0001026"},{"id":"T13098","span":{"begin":626,"end":633},"obj":"SO:0000112"},{"id":"T13099","span":{"begin":701,"end":705},"obj":"SO:0000147"},{"id":"T13100","span":{"begin":712,"end":718},"obj":"PR:000004372"},{"id":"T13101","span":{"begin":744,"end":758},"obj":"SO:0000010"}],"text":"In situ hybridization was carried out on 12-μm paraffin sections using digoxigenin-labeled RNA probes (Roche Diagnostics, Indianapolis, Indiana, United States) according to standard protocols (Wilkinson and Nieto 1993). Embryos and postnatal skin samples were obtained from intercrosses of deH/+ mice. Embryos E13.5 or younger were fixed for 24 h; those older than E13.5 and postnatal skin were fixed for 36–48 h prior to embedding. The Tbx15 probe was generated by RT–PCR using primers GGCGGCTAAAATGAGTGAAC and TGCCTGCTTTGGTGATGAT (corresponds to exons 1 and 2), and the En1 probe was generated by PCR from genomic DNA using primers ACGCACCAGGAAGCTAAAGA and AGCAACGAAAACGAAACTGG (located in the last exon). The Agouti probe corresponds to the protein-coding sequence."}

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{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T13103","span":{"begin":8,"end":21},"obj":"GO:0097617"},{"id":"T13104","span":{"begin":71,"end":82},"obj":"CHEBI:42098"},{"id":"T13105","span":{"begin":83,"end":90},"obj":"CHEBI_SO_EXT:molecular_label_or_mark_or_tag_process"},{"id":"T13106","span":{"begin":91,"end":101},"obj":"CHEBI_SO_EXT:riboprobe"},{"id":"T13107","span":{"begin":220,"end":227},"obj":"UBERON:0000922"},{"id":"T13108","span":{"begin":236,"end":241},"obj":"GO:0007567"},{"id":"T13109","span":{"begin":242,"end":246},"obj":"UBERON_EXT:zone_of_skin_or_skin_of_body"},{"id":"T13110","span":{"begin":294,"end":295},"obj":"SO_EXT:normal_or_wild_type_or_present"},{"id":"T13111","span":{"begin":296,"end":300},"obj":"NCBITaxon:10088"},{"id":"T13112","span":{"begin":302,"end":309},"obj":"UBERON:0000922"},{"id":"T13113","span":{"begin":379,"end":384},"obj":"GO:0007567"},{"id":"T13114","span":{"begin":385,"end":389},"obj":"UBERON_EXT:zone_of_skin_or_skin_of_body"},{"id":"T13115","span":{"begin":437,"end":442},"obj":"PR_EXT:000016145"},{"id":"T13116","span":{"begin":443,"end":448},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T13117","span":{"begin":466,"end":468},"obj":"GO_EXT:reverse_transcription_or_reverse_transcriptase"},{"id":"T13118","span":{"begin":479,"end":486},"obj":"SO_EXT:0000112"},{"id":"T13119","span":{"begin":548,"end":553},"obj":"SO_EXT:0000147"},{"id":"T13120","span":{"begin":572,"end":575},"obj":"PR_EXT:000007070"},{"id":"T13121","span":{"begin":576,"end":581},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T13122","span":{"begin":608,"end":619},"obj":"SO_EXT:genomic_DNA"},{"id":"T13123","span":{"begin":616,"end":619},"obj":"CHEBI_SO_EXT:DNA"},{"id":"T13124","span":{"begin":626,"end":633},"obj":"SO_EXT:0000112"},{"id":"T13125","span":{"begin":701,"end":705},"obj":"SO_EXT:0000147"},{"id":"T13126","span":{"begin":712,"end":718},"obj":"PR_EXT:000004372"},{"id":"T13127","span":{"begin":719,"end":724},"obj":"CHEBI_SO_EXT:molecular_probe"},{"id":"T13128","span":{"begin":744,"end":751},"obj":"CHEBI_PR_EXT:protein"},{"id":"T13129","span":{"begin":744,"end":758},"obj":"SO:0000010"},{"id":"T13130","span":{"begin":752,"end":767},"obj":"SO_EXT:coding_sequence"}],"text":"In situ hybridization was carried out on 12-μm paraffin sections using digoxigenin-labeled RNA probes (Roche Diagnostics, Indianapolis, Indiana, United States) according to standard protocols (Wilkinson and Nieto 1993). Embryos and postnatal skin samples were obtained from intercrosses of deH/+ mice. Embryos E13.5 or younger were fixed for 24 h; those older than E13.5 and postnatal skin were fixed for 36–48 h prior to embedding. The Tbx15 probe was generated by RT–PCR using primers GGCGGCTAAAATGAGTGAAC and TGCCTGCTTTGGTGATGAT (corresponds to exons 1 and 2), and the En1 probe was generated by PCR from genomic DNA using primers ACGCACCAGGAAGCTAAAGA and AGCAACGAAAACGAAACTGG (located in the last exon). The Agouti probe corresponds to the protein-coding sequence."}