PMC:314463 / 60197-60440 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/314463","sourcedb":"PMC","sourceid":"314463","source_url":"http://www.ncbi.nlm.nih.gov/pmc/314463","text":" In brief, an IRES-LacZ-neo cassette with 5′ and 3′ homology arms of 3.5 kb and 1.8 kb was inserted into a unique BamHI site that lies 479 nucleotides downstream of the transcriptional initiation site (relative to the mRNA sequence) in exon 3.","tracks":[{"project":"craft-sa-dev","denotations":[{"id":"T12999","span":{"begin":91,"end":99},"obj":"VBN"},{"id":"T13000","span":{"begin":4,"end":9},"obj":"NN"},{"id":"T13001","span":{"begin":9,"end":11},"obj":","},{"id":"T13002","span":{"begin":11,"end":13},"obj":"DT"},{"id":"T13003","span":{"begin":28,"end":36},"obj":"NN"},{"id":"T13004","span":{"begin":14,"end":18},"obj":"NN"},{"id":"T13005","span":{"begin":24,"end":27},"obj":"NN"},{"id":"T13006","span":{"begin":18,"end":19},"obj":"HYPH"},{"id":"T13007","span":{"begin":19,"end":23},"obj":"NN"},{"id":"T13008","span":{"begin":23,"end":24},"obj":"HYPH"},{"id":"T13009","span":{"begin":37,"end":41},"obj":"IN"},{"id":"T13010","span":{"begin":42,"end":43},"obj":"CD"},{"id":"T13011","span":{"begin":61,"end":65},"obj":"NNS"},{"id":"T13012","span":{"begin":43,"end":44},"obj":"SYM"},{"id":"T13013","span":{"begin":45,"end":48},"obj":"CC"},{"id":"T13014","span":{"begin":49,"end":50},"obj":"CD"},{"id":"T13015","span":{"begin":50,"end":51},"obj":"SYM"},{"id":"T13016","span":{"begin":52,"end":60},"obj":"NN"},{"id":"T13017","span":{"begin":66,"end":68},"obj":"IN"},{"id":"T13018","span":{"begin":69,"end":72},"obj":"CD"},{"id":"T13019","span":{"begin":73,"end":75},"obj":"NN"},{"id":"T13020","span":{"begin":76,"end":79},"obj":"CC"},{"id":"T13021","span":{"begin":80,"end":83},"obj":"CD"},{"id":"T13022","span":{"begin":84,"end":86},"obj":"NN"},{"id":"T13023","span":{"begin":87,"end":90},"obj":"VBD"},{"id":"T13024","span":{"begin":100,"end":104},"obj":"IN"},{"id":"T13025","span":{"begin":105,"end":106},"obj":"DT"},{"id":"T13026","span":{"begin":120,"end":124},"obj":"NN"},{"id":"T13027","span":{"begin":107,"end":113},"obj":"JJ"},{"id":"T13028","span":{"begin":114,"end":119},"obj":"NN"},{"id":"T13029","span":{"begin":125,"end":129},"obj":"WDT"},{"id":"T13030","span":{"begin":130,"end":134},"obj":"VBZ"},{"id":"T13031","span":{"begin":135,"end":138},"obj":"CD"},{"id":"T13032","span":{"begin":139,"end":150},"obj":"NNS"},{"id":"T13033","span":{"begin":151,"end":161},"obj":"RB"},{"id":"T13034","span":{"begin":162,"end":164},"obj":"IN"},{"id":"T13035","span":{"begin":165,"end":168},"obj":"DT"},{"id":"T13036","span":{"begin":196,"end":200},"obj":"NN"},{"id":"T13037","span":{"begin":169,"end":184},"obj":"JJ"},{"id":"T13038","span":{"begin":185,"end":195},"obj":"NN"},{"id":"T13039","span":{"begin":201,"end":202},"obj":"-LRB-"},{"id":"T13040","span":{"begin":202,"end":210},"obj":"JJ"},{"id":"T13041","span":{"begin":211,"end":213},"obj":"IN"},{"id":"T13042","span":{"begin":214,"end":217},"obj":"DT"},{"id":"T13043","span":{"begin":223,"end":231},"obj":"NN"},{"id":"T13044","span":{"begin":218,"end":222},"obj":"NN"},{"id":"T13045","span":{"begin":231,"end":232},"obj":"-RRB-"},{"id":"T13046","span":{"begin":233,"end":235},"obj":"IN"},{"id":"T13047","span":{"begin":236,"end":240},"obj":"NN"},{"id":"T13048","span":{"begin":241,"end":242},"obj":"CD"},{"id":"T13049","span":{"begin":242,"end":243},"obj":"."},{"id":"T12997","span":{"begin":0,"end":243},"obj":"sentence"},{"id":"T12998","span":{"begin":1,"end":3},"obj":"IN"}],"relations":[{"id":"R9103","pred":"prep","subj":"T12998","obj":"T12999"},{"id":"R9104","pred":"pobj","subj":"T13000","obj":"T12998"},{"id":"R9105","pred":"punct","subj":"T13001","obj":"T12999"},{"id":"R9106","pred":"det","subj":"T13002","obj":"T13003"},{"id":"R9107","pred":"nsubjpass","subj":"T13003","obj":"T12999"},{"id":"R9108","pred":"compound","subj":"T13004","obj":"T13005"},{"id":"R9109","pred":"compound","subj":"T13005","obj":"T13003"},{"id":"R9110","pred":"punct","subj":"T13006","obj":"T13005"},{"id":"R9111","pred":"compound","subj":"T13007","obj":"T13005"},{"id":"R9112","pred":"punct","subj":"T13008","obj":"T13005"},{"id":"R9113","pred":"prep","subj":"T13009","obj":"T13003"},{"id":"R9114","pred":"nummod","subj":"T13010","obj":"T13011"},{"id":"R9115","pred":"pobj","subj":"T13011","obj":"T13009"},{"id":"R9116","pred":"punct","subj":"T13012","obj":"T13010"},{"id":"R9117","pred":"cc","subj":"T13013","obj":"T13010"},{"id":"R9118","pred":"conj","subj":"T13014","obj":"T13010"},{"id":"R9119","pred":"punct","subj":"T13015","obj":"T13014"},{"id":"R9120","pred":"compound","subj":"T13016","obj":"T13011"},{"id":"R9121","pred":"prep","subj":"T13017","obj":"T13011"},{"id":"R9122","pred":"nummod","subj":"T13018","obj":"T13019"},{"id":"R9123","pred":"pobj","subj":"T13019","obj":"T13017"},{"id":"R9124","pred":"cc","subj":"T13020","obj":"T13019"},{"id":"R9125","pred":"nummod","subj":"T13021","obj":"T13022"},{"id":"R9126","pred":"conj","subj":"T13022","obj":"T13019"},{"id":"R9127","pred":"auxpass","subj":"T13023","obj":"T12999"},{"id":"R9128","pred":"prep","subj":"T13024","obj":"T12999"},{"id":"R9129","pred":"det","subj":"T13025","obj":"T13026"},{"id":"R9130","pred":"pobj","subj":"T13026","obj":"T13024"},{"id":"R9131","pred":"amod","subj":"T13027","obj":"T13026"},{"id":"R9132","pred":"compound","subj":"T13028","obj":"T13026"},{"id":"R9133","pred":"dep","subj":"T13029","obj":"T13030"},{"id":"R9134","pred":"relcl","subj":"T13030","obj":"T13026"},{"id":"R9135","pred":"nummod","subj":"T13031","obj":"T13032"},{"id":"R9136","pred":"npadvmod","subj":"T13032","obj":"T13033"},{"id":"R9137","pred":"advmod","subj":"T13033","obj":"T13030"},{"id":"R9138","pred":"prep","subj":"T13034","obj":"T13033"},{"id":"R9139","pred":"det","subj":"T13035","obj":"T13036"},{"id":"R9140","pred":"pobj","subj":"T13036","obj":"T13034"},{"id":"R9141","pred":"amod","subj":"T13037","obj":"T13036"},{"id":"R9142","pred":"compound","subj":"T13038","obj":"T13036"},{"id":"R9143","pred":"punct","subj":"T13039","obj":"T13030"},{"id":"R9144","pred":"advcl","subj":"T13040","obj":"T13030"},{"id":"R9145","pred":"prep","subj":"T13041","obj":"T13040"},{"id":"R9146","pred":"det","subj":"T13042","obj":"T13043"},{"id":"R9147","pred":"pobj","subj":"T13043","obj":"T13041"},{"id":"R9148","pred":"compound","subj":"T13044","obj":"T13043"},{"id":"R9149","pred":"punct","subj":"T13045","obj":"T13030"},{"id":"R9150","pred":"prep","subj":"T13046","obj":"T13030"},{"id":"R9151","pred":"pobj","subj":"T13047","obj":"T13046"},{"id":"R9152","pred":"nummod","subj":"T13048","obj":"T13047"},{"id":"R9153","pred":"punct","subj":"T13049","obj":"T12999"}],"attributes":[{"subj":"T12999","pred":"source","obj":"craft-sa-dev"},{"subj":"T13000","pred":"source","obj":"craft-sa-dev"},{"subj":"T13001","pred":"source","obj":"craft-sa-dev"},{"subj":"T13002","pred":"source","obj":"craft-sa-dev"},{"subj":"T13003","pred":"source","obj":"craft-sa-dev"},{"subj":"T13004","pred":"source","obj":"craft-sa-dev"},{"subj":"T13005","pred":"source","obj":"craft-sa-dev"},{"subj":"T13006","pred":"source","obj":"craft-sa-dev"},{"subj":"T13007","pred":"source","obj":"craft-sa-dev"},{"subj":"T13008","pred":"source","obj":"craft-sa-dev"},{"subj":"T13009","pred":"source","obj":"craft-sa-dev"},{"subj":"T13010","pred":"source","obj":"craft-sa-dev"},{"subj":"T13011","pred":"source","obj":"craft-sa-dev"},{"subj":"T13012","pred":"source","obj":"craft-sa-dev"},{"subj":"T13013","pred":"source","obj":"craft-sa-dev"},{"subj":"T13014","pred":"source","obj":"craft-sa-dev"},{"subj":"T13015","pred":"source","obj":"craft-sa-dev"},{"subj":"T13016","pred":"source","obj":"craft-sa-dev"},{"subj":"T13017","pred":"source","obj":"craft-sa-dev"},{"subj":"T13018","pred":"source","obj":"craft-sa-dev"},{"subj":"T13019","pred":"source","obj":"craft-sa-dev"},{"subj":"T13020","pred":"source","obj":"craft-sa-dev"},{"subj":"T13021","pred":"source","obj":"craft-sa-dev"},{"subj":"T13022","pred":"source","obj":"craft-sa-dev"},{"subj":"T13023","pred":"source","obj":"craft-sa-dev"},{"subj":"T13024","pred":"source","obj":"craft-sa-dev"},{"subj":"T13025","pred":"source","obj":"craft-sa-dev"},{"subj":"T13026","pred":"source","obj":"craft-sa-dev"},{"subj":"T13027","pred":"source","obj":"craft-sa-dev"},{"subj":"T13028","pred":"source","obj":"craft-sa-dev"},{"subj":"T13029","pred":"source","obj":"craft-sa-dev"},{"subj":"T13030","pred":"source","obj":"craft-sa-dev"},{"subj":"T13031","pred":"source","obj":"craft-sa-dev"},{"subj":"T13032","pred":"source","obj":"craft-sa-dev"},{"subj":"T13033","pred":"source","obj":"craft-sa-dev"},{"subj":"T13034","pred":"source","obj":"craft-sa-dev"},{"subj":"T13035","pred":"source","obj":"craft-sa-dev"},{"subj":"T13036","pred":"source","obj":"craft-sa-dev"},{"subj":"T13037","pred":"source","obj":"craft-sa-dev"},{"subj":"T13038","pred":"source","obj":"craft-sa-dev"},{"subj":"T13039","pred":"source","obj":"craft-sa-dev"},{"subj":"T13040","pred":"source","obj":"craft-sa-dev"},{"subj":"T13041","pred":"source","obj":"craft-sa-dev"},{"subj":"T13042","pred":"source","obj":"craft-sa-dev"},{"subj":"T13043","pred":"source","obj":"craft-sa-dev"},{"subj":"T13044","pred":"source","obj":"craft-sa-dev"},{"subj":"T13045","pred":"source","obj":"craft-sa-dev"},{"subj":"T13046","pred":"source","obj":"craft-sa-dev"},{"subj":"T13047","pred":"source","obj":"craft-sa-dev"},{"subj":"T13048","pred":"source","obj":"craft-sa-dev"},{"subj":"T13049","pred":"source","obj":"craft-sa-dev"},{"subj":"T12997","pred":"source","obj":"craft-sa-dev"},{"subj":"T12998","pred":"source","obj":"craft-sa-dev"}]},{"project":"craft-ca-core-dev","denotations":[{"id":"T12940","span":{"begin":14,"end":18},"obj":"SO:0000243"},{"id":"T12941","span":{"begin":19,"end":23},"obj":"PR:000033987"},{"id":"T12942","span":{"begin":28,"end":36},"obj":"SO:0005853"},{"id":"T12943","span":{"begin":114,"end":119},"obj":"PR:P23940"},{"id":"T12944","span":{"begin":169,"end":200},"obj":"SO:0000315"},{"id":"T12945","span":{"begin":236,"end":240},"obj":"SO:0000147"}],"attributes":[{"subj":"T12940","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T12941","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T12942","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T12943","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T12944","pred":"source","obj":"craft-ca-core-dev"},{"subj":"T12945","pred":"source","obj":"craft-ca-core-dev"}]},{"project":"craft-ca-core-ex-dev","denotations":[{"id":"T12953","span":{"begin":14,"end":18},"obj":"SO_EXT:0000243"},{"id":"T12954","span":{"begin":19,"end":23},"obj":"PR_EXT:000033987"},{"id":"T12955","span":{"begin":24,"end":27},"obj":"CHEBI_GO_EXT:neomycin_or_neomycin_phosphotransferase"},{"id":"T12956","span":{"begin":28,"end":36},"obj":"SO_EXT:0005853"},{"id":"T12957","span":{"begin":74,"end":75},"obj":"CHEBI_SO_EXT:base"},{"id":"T12958","span":{"begin":85,"end":86},"obj":"CHEBI_SO_EXT:base"},{"id":"T12959","span":{"begin":91,"end":99},"obj":"SO_EXT:sequence_insertion_process"},{"id":"T12960","span":{"begin":114,"end":119},"obj":"PR_EXT:P23940"},{"id":"T12961","span":{"begin":139,"end":150},"obj":"CHEBI_SO_EXT:nucleotide"},{"id":"T12962","span":{"begin":151,"end":161},"obj":"SO_EXT:sequence_downstreamness"},{"id":"T12963","span":{"begin":169,"end":184},"obj":"GO_EXT:transcription"},{"id":"T12964","span":{"begin":169,"end":200},"obj":"SO_EXT:0000315"},{"id":"T12965","span":{"begin":218,"end":222},"obj":"CHEBI_SO_EXT:mRNA"},{"id":"T12966","span":{"begin":223,"end":231},"obj":"SO_EXT:biological_sequence"},{"id":"T12967","span":{"begin":236,"end":240},"obj":"SO_EXT:0000147"}],"attributes":[{"subj":"T12953","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12954","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12955","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12956","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12957","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12958","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12959","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12960","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12961","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12962","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12963","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12964","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12965","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12966","pred":"source","obj":"craft-ca-core-ex-dev"},{"subj":"T12967","pred":"source","obj":"craft-ca-core-ex-dev"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"craft-sa-dev","color":"#d693ec","default":true},{"id":"craft-ca-core-dev","color":"#93ecbc"},{"id":"craft-ca-core-ex-dev","color":"#eca293"}]}]}}