PMC:3062687 / 32510-32947
Annnotations
bionlp-st-ge-2016-uniprot
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2_test
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pmc-enju-pas
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bionlp-st-ge-2016-spacy-parsed
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GO-CC
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sentences
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events-check-again
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bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T22187","span":{"begin":0,"end":10},"obj":"Protein"},{"id":"T22188","span":{"begin":32,"end":42},"obj":"Protein"},{"id":"T22189","span":{"begin":187,"end":216},"obj":"Protein"},{"id":"T22190","span":{"begin":224,"end":248},"obj":"Protein"},{"id":"T22191","span":{"begin":407,"end":426},"obj":"Protein"},{"id":"T22192","span":{"begin":428,"end":435},"obj":"Protein"},{"id":"T22193","span":{"begin":397,"end":402},"obj":"Gene_expression"},{"id":"T22194","span":{"begin":397,"end":402},"obj":"Gene_expression"}],"relations":[{"id":"R17697","pred":"themeOf","subj":"T22191","obj":"T22193"},{"id":"R17698","pred":"themeOf","subj":"T22192","obj":"T22194"}],"text":"Luciferase Reporter Gene Assays\nLuciferase reporter gene assays were performed as previously described6. Briefly, cells were cotransfected at a ratio of 10:1 with various promoter-driven firefly luciferase constructs to the Renilla luciferase pTKRL plasmid, together with indicated plasmids. Cells were cultured for 1–2 days and then stimulated in triplicate before harvest. Lysates were analyzed using the Dual-Luciferase Kit (Promega)."}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T22035","span":{"begin":232,"end":242},"obj":"Protein"},{"id":"T22034","span":{"begin":195,"end":205},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Luciferase Reporter Gene Assays\nLuciferase reporter gene assays were performed as previously described6. Briefly, cells were cotransfected at a ratio of 10:1 with various promoter-driven firefly luciferase constructs to the Renilla luciferase pTKRL plasmid, together with indicated plasmids. Cells were cultured for 1–2 days and then stimulated in triplicate before harvest. Lysates were analyzed using the Dual-Luciferase Kit (Promega)."}
test2
{"project":"test2","denotations":[{"id":"T22032","span":{"begin":195,"end":205},"obj":"Protein"},{"id":"T22033","span":{"begin":232,"end":242},"obj":"Protein"}],"text":"Luciferase Reporter Gene Assays\nLuciferase reporter gene assays were performed as previously described6. Briefly, cells were cotransfected at a ratio of 10:1 with various promoter-driven firefly luciferase constructs to the Renilla luciferase pTKRL plasmid, together with indicated plasmids. Cells were cultured for 1–2 days and then stimulated in triplicate before harvest. Lysates were analyzed using the Dual-Luciferase Kit (Promega)."}