PMC:3062687 / 31774-32508 JSON TXT

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bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T21862","span":{"begin":189,"end":191},"obj":"P0A7Z4"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Subcellular Fractionation\nSubcellular fractionation was performed by differential centrifugation as previously described6. Briefly, cells were resuspended in ice-cold Buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1 mM EDTA, 0.5 mM DTT, 0.4 % NP-40, 0.5 mM PMSF, complete protease inhibitor cocktail) at 4 °C for 5 min. Lysates were centrifuged at 4 °C, 500 × g for 3 min, and supernatants were collected as cytosolic fractions. Pellets were incubated in Buffer C (20 mM HEPES pH7.9, 420 mM NaCl, 1.5 mM MgCl2, 25% glycerol, 0.5 mM PMSF, 0.2 mM EDTA, 0.5 mM DTT, complete protease inhibitor cocktail) at 4 °C for 10 min. Supernatants were collected as nuclear fractions following a centrifuge at 4 °C, 13,800 × g for 10 min."}

2_test

{"project":"2_test","denotations":[{"id":"21399639-18045535-74765188","span":{"begin":120,"end":121},"obj":"18045535"},{"id":"21399639-18045535-74765188","span":{"begin":120,"end":121},"obj":"18045535"}],"text":"Subcellular Fractionation\nSubcellular fractionation was performed by differential centrifugation as previously described6. Briefly, cells were resuspended in ice-cold Buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1 mM EDTA, 0.5 mM DTT, 0.4 % NP-40, 0.5 mM PMSF, complete protease inhibitor cocktail) at 4 °C for 5 min. Lysates were centrifuged at 4 °C, 500 × g for 3 min, and supernatants were collected as cytosolic fractions. Pellets were incubated in Buffer C (20 mM HEPES pH7.9, 420 mM NaCl, 1.5 mM MgCl2, 25% glycerol, 0.5 mM PMSF, 0.2 mM EDTA, 0.5 mM DTT, complete protease inhibitor cocktail) at 4 °C for 10 min. Supernatants were collected as nuclear fractions following a centrifuge at 4 °C, 13,800 × g for 10 min."}

pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

GO-CC

{"project":"GO-CC","denotations":[{"id":"T22029","span":{"begin":132,"end":137},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Subcellular Fractionation\nSubcellular fractionation was performed by differential centrifugation as previously described6. Briefly, cells were resuspended in ice-cold Buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1 mM EDTA, 0.5 mM DTT, 0.4 % NP-40, 0.5 mM PMSF, complete protease inhibitor cocktail) at 4 °C for 5 min. Lysates were centrifuged at 4 °C, 500 × g for 3 min, and supernatants were collected as cytosolic fractions. Pellets were incubated in Buffer C (20 mM HEPES pH7.9, 420 mM NaCl, 1.5 mM MgCl2, 25% glycerol, 0.5 mM PMSF, 0.2 mM EDTA, 0.5 mM DTT, complete protease inhibitor cocktail) at 4 °C for 10 min. Supernatants were collected as nuclear fractions following a centrifuge at 4 °C, 13,800 × g for 10 min."}

sentences

{"project":"sentences","denotations":[{"id":"T21706","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T21707","span":{"begin":26,"end":122},"obj":"Sentence"},{"id":"T21708","span":{"begin":123,"end":329},"obj":"Sentence"},{"id":"T21709","span":{"begin":330,"end":438},"obj":"Sentence"},{"id":"T21710","span":{"begin":439,"end":630},"obj":"Sentence"},{"id":"T21711","span":{"begin":631,"end":734},"obj":"Sentence"},{"id":"T220","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T221","span":{"begin":26,"end":122},"obj":"Sentence"},{"id":"T222","span":{"begin":123,"end":329},"obj":"Sentence"},{"id":"T223","span":{"begin":330,"end":438},"obj":"Sentence"},{"id":"T224","span":{"begin":439,"end":630},"obj":"Sentence"},{"id":"T225","span":{"begin":631,"end":734},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Subcellular Fractionation\nSubcellular fractionation was performed by differential centrifugation as previously described6. Briefly, cells were resuspended in ice-cold Buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1 mM EDTA, 0.5 mM DTT, 0.4 % NP-40, 0.5 mM PMSF, complete protease inhibitor cocktail) at 4 °C for 5 min. Lysates were centrifuged at 4 °C, 500 × g for 3 min, and supernatants were collected as cytosolic fractions. Pellets were incubated in Buffer C (20 mM HEPES pH7.9, 420 mM NaCl, 1.5 mM MgCl2, 25% glycerol, 0.5 mM PMSF, 0.2 mM EDTA, 0.5 mM DTT, complete protease inhibitor cocktail) at 4 °C for 10 min. Supernatants were collected as nuclear fractions following a centrifuge at 4 °C, 13,800 × g for 10 min."}