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PMC:3062687 / 31774-32508 JSONTXT

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bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T21862 189-191 P0A7Z4 denotes pH

2_test

Id Subject Object Predicate Lexical cue
21399639-18045535-74765188 120-121 18045535 denotes 6
21399639-18045535-74765188 120-121 18045535 denotes 6

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T21712 0-11 JJ denotes Subcellular
T21713 12-25 NN denotes Fractionation
T21714 26-37 JJ denotes Subcellular
T21715 38-51 NN denotes fractionation
T21716 52-55 VB denotes was
T21717 56-65 VB denotes performed
T21718 66-68 IN denotes by
T21719 69-81 JJ denotes differential
T21720 82-96 NN denotes centrifugation
T21721 97-99 IN denotes as
T21722 100-110 RB denotes previously
T21723 111-121 NN denotes described6
T21724 123-130 RB denotes Briefly
T21725 130-131 -COMMA- denotes ,
T21726 132-137 NN denotes cells
T21727 138-142 VB denotes were
T21728 143-154 VB denotes resuspended
T21729 155-157 IN denotes in
T21730 158-166 JJ denotes ice-cold
T21731 167-173 NN denotes Buffer
T21732 174-175 NN denotes A
T21733 176-177 -LRB- denotes (
T21734 177-179 CD denotes 10
T21735 180-182 NN denotes mM
T21736 183-188 NN denotes HEPES
T21737 189-191 NN denotes pH
T21738 192-195 CD denotes 7.9
T21739 195-196 -COMMA- denotes ,
T21740 197-199 CD denotes 10
T21741 200-202 NN denotes mM
T21742 203-206 NN denotes KCl
T21743 206-207 -COMMA- denotes ,
T21744 208-211 CD denotes 1.5
T21745 212-214 NN denotes mM
T21746 215-220 NN denotes MgCl2
T21747 220-221 -COMMA- denotes ,
T21748 222-225 CD denotes 0.1
T21749 226-228 NN denotes mM
T21750 229-233 NN denotes EDTA
T21751 233-234 -COMMA- denotes ,
T21752 235-238 CD denotes 0.5
T21753 239-241 NN denotes mM
T21754 242-245 NN denotes DTT
T21755 245-246 -COMMA- denotes ,
T21756 247-250 CD denotes 0.4
T21757 251-252 NN denotes %
T21758 253-258 NN denotes NP-40
T21759 258-259 -COMMA- denotes ,
T21760 260-263 CD denotes 0.5
T21761 264-266 NN denotes mM
T21762 267-271 NN denotes PMSF
T21763 271-272 -COMMA- denotes ,
T21764 273-281 JJ denotes complete
T21765 282-290 NN denotes protease
T21766 291-300 NN denotes inhibitor
T21767 301-309 NN denotes cocktail
T21768 309-310 -RRB- denotes )
T21769 311-313 IN denotes at
T21770 314-315 CD denotes 4
T21771 316-318 NN denotes °C
T21772 319-322 IN denotes for
T21773 323-324 CD denotes 5
T21774 325-328 NN denotes min
T21775 330-337 NN denotes Lysates
T21776 338-342 VB denotes were
T21777 343-354 VB denotes centrifuged
T21778 355-357 IN denotes at
T21779 358-359 CD denotes 4
T21780 360-362 NN denotes °C
T21781 362-363 -COMMA- denotes ,
T21782 364-367 CD denotes 500
T21783 368-369 SYM denotes ×
T21784 370-371 NN denotes g
T21785 372-375 IN denotes for
T21786 376-377 CD denotes 3
T21787 378-381 NN denotes min
T21788 381-382 -COMMA- denotes ,
T21789 383-386 CC denotes and
T21790 387-399 NN denotes supernatants
T21791 400-404 VB denotes were
T21792 405-414 VB denotes collected
T21793 415-417 IN denotes as
T21794 418-427 JJ denotes cytosolic
T21795 428-437 NN denotes fractions
T21796 439-446 NN denotes Pellets
T21797 447-451 VB denotes were
T21798 452-461 VB denotes incubated
T21799 462-464 IN denotes in
T21800 465-471 NNP denotes Buffer
T21801 472-473 NNP denotes C
T21802 474-475 -LRB- denotes (
T21803 475-477 CD denotes 20
T21804 478-480 NN denotes mM
T21805 481-486 NN denotes HEPES
T21806 487-492 NN denotes pH7.9
T21807 492-493 -COMMA- denotes ,
T21808 494-497 CD denotes 420
T21809 498-500 NN denotes mM
T21810 501-505 NN denotes NaCl
T21811 505-506 -COMMA- denotes ,
T21812 507-510 CD denotes 1.5
T21813 511-513 NN denotes mM
T21814 514-519 NN denotes MgCl2
T21815 519-520 -COMMA- denotes ,
T21816 521-523 CD denotes 25
T21817 523-524 NN denotes %
T21818 525-533 NN denotes glycerol
T21819 533-534 -COMMA- denotes ,
T21820 535-538 CD denotes 0.5
T21821 539-541 NN denotes mM
T21822 542-546 NN denotes PMSF
T21823 546-547 -COMMA- denotes ,
T21824 548-551 CD denotes 0.2
T21825 552-554 NN denotes mM
T21826 555-559 NN denotes EDTA
T21827 559-560 -COMMA- denotes ,
T21828 561-564 CD denotes 0.5
T21829 565-567 NN denotes mM
T21830 568-571 NN denotes DTT
T21831 571-572 -COMMA- denotes ,
T21832 573-581 JJ denotes complete
T21833 582-590 NN denotes protease
T21834 591-600 NN denotes inhibitor
T21835 601-609 NN denotes cocktail
T21836 609-610 -RRB- denotes )
T21837 611-613 IN denotes at
T21838 614-615 CD denotes 4
T21839 616-618 NN denotes °C
T21840 619-622 IN denotes for
T21841 623-625 CD denotes 10
T21842 626-629 NN denotes min
T21843 631-643 NN denotes Supernatants
T21844 644-648 VB denotes were
T21845 649-658 VB denotes collected
T21846 659-661 IN denotes as
T21847 662-669 JJ denotes nuclear
T21848 670-679 NN denotes fractions
T21849 680-689 VB denotes following
T21850 690-691 DT denotes a
T21851 692-702 NN denotes centrifuge
T21852 703-705 IN denotes at
T21853 706-707 CD denotes 4
T21854 708-710 NN denotes °C
T21855 710-711 -COMMA- denotes ,
T21856 712-718 CD denotes 13,800
T21857 719-720 SYM denotes ×
T21858 721-722 NN denotes g
T21859 723-726 IN denotes for
T21860 727-729 CD denotes 10
T21861 730-733 NN denotes min
R17254 T21713 T21712 arg1Of Fractionation,Subcellular
R17255 T21715 T21714 arg1Of fractionation,Subcellular
R17256 T21715 T21716 arg1Of fractionation,was
R17257 T21715 T21717 arg2Of fractionation,performed
R17258 T21717 T21716 arg2Of performed,was
R17259 T21717 T21721 arg1Of performed,as
R17260 T21720 T21717 arg1Of centrifugation,performed
R17261 T21720 T21718 arg2Of centrifugation,by
R17262 T21720 T21719 arg1Of centrifugation,differential
R17263 T21723 T21721 arg2Of described6,as
R17264 T21723 T21722 arg1Of described6,previously
R17265 T21726 T21727 arg1Of cells,were
R17266 T21726 T21728 arg2Of cells,resuspended
R17267 T21728 T21724 arg1Of resuspended,Briefly
R17268 T21728 T21725 arg1Of resuspended,","
R17269 T21728 T21727 arg2Of resuspended,were
R17270 T21728 T21729 arg1Of resuspended,in
R17271 T21728 T21733 arg1Of resuspended,(
R17272 T21728 T21769 arg1Of resuspended,at
R17273 T21728 T21772 arg1Of resuspended,for
R17274 T21732 T21729 arg2Of A,in
R17275 T21732 T21730 arg1Of A,ice-cold
R17276 T21732 T21731 arg1Of A,Buffer
R17277 T21734 T21735 arg1Of 10,mM
R17278 T21737 T21736 arg1Of pH,HEPES
R17279 T21737 T21738 arg1Of pH,7.9
R17280 T21737 T21739 arg1Of pH,","
R17281 T21739 T21743 arg1Of ",",","
R17282 T21740 T21741 arg1Of 10,mM
R17283 T21742 T21739 arg2Of KCl,","
R17284 T21742 T21740 arg1Of KCl,10
R17285 T21743 T21747 arg1Of ",",","
R17286 T21744 T21745 arg1Of 1.5,mM
R17287 T21746 T21743 arg2Of MgCl2,","
R17288 T21746 T21744 arg1Of MgCl2,1.5
R17289 T21747 T21751 arg1Of ",",","
R17290 T21748 T21749 arg1Of 0.1,mM
R17291 T21750 T21747 arg2Of EDTA,","
R17292 T21750 T21748 arg1Of EDTA,0.1
R17293 T21751 T21733 arg2Of ",",(
R17294 T21751 T21734 arg1Of ",",10
R17295 T21752 T21753 arg1Of 0.5,mM
R17296 T21754 T21752 arg1Of DTT,0.5
R17297 T21754 T21755 arg1Of DTT,","
R17298 T21755 T21759 arg1Of ",",","
R17299 T21756 T21757 arg1Of 0.4,%
R17300 T21758 T21755 arg2Of NP-40,","
R17301 T21758 T21756 arg1Of NP-40,0.4
R17302 T21759 T21763 arg1Of ",",","
R17303 T21760 T21761 arg1Of 0.5,mM
R17304 T21762 T21759 arg2Of PMSF,","
R17305 T21762 T21760 arg1Of PMSF,0.5
R17306 T21763 T21751 arg2Of ",",","
R17307 T21767 T21763 arg2Of cocktail,","
R17308 T21767 T21764 arg1Of cocktail,complete
R17309 T21767 T21765 arg1Of cocktail,protease
R17310 T21767 T21766 arg1Of cocktail,inhibitor
R17311 T21768 T21733 arg3Of ),(
R17312 T21771 T21769 arg2Of °C,at
R17313 T21771 T21770 arg1Of °C,4
R17314 T21774 T21772 arg2Of min,for
R17315 T21774 T21773 arg1Of min,5
R17316 T21775 T21776 arg1Of Lysates,were
R17317 T21775 T21777 arg2Of Lysates,centrifuged
R17318 T21777 T21776 arg2Of centrifuged,were
R17319 T21777 T21778 arg1Of centrifuged,at
R17320 T21777 T21789 arg1Of centrifuged,and
R17321 T21780 T21779 arg1Of °C,4
R17322 T21780 T21781 arg1Of °C,","
R17323 T21781 T21778 arg2Of ",",at
R17324 T21784 T21781 arg2Of g,","
R17325 T21784 T21782 arg1Of g,500
R17326 T21784 T21783 arg1Of g,×
R17327 T21784 T21785 arg1Of g,for
R17328 T21787 T21785 arg2Of min,for
R17329 T21787 T21786 arg1Of min,3
R17330 T21789 T21788 arg1Of and,","
R17331 T21790 T21791 arg1Of supernatants,were
R17332 T21790 T21792 arg2Of supernatants,collected
R17333 T21792 T21789 arg2Of collected,and
R17334 T21792 T21791 arg2Of collected,were
R17335 T21792 T21793 arg1Of collected,as
R17336 T21795 T21793 arg2Of fractions,as
R17337 T21795 T21794 arg1Of fractions,cytosolic
R17338 T21796 T21797 arg1Of Pellets,were
R17339 T21796 T21798 arg2Of Pellets,incubated
R17340 T21798 T21797 arg2Of incubated,were
R17341 T21798 T21799 arg1Of incubated,in
R17342 T21801 T21799 arg2Of C,in
R17343 T21801 T21800 arg1Of C,Buffer
R17344 T21801 T21802 arg1Of C,(
R17345 T21801 T21837 arg1Of C,at
R17346 T21806 T21803 arg1Of pH7.9,20
R17347 T21806 T21804 arg1Of pH7.9,mM
R17348 T21806 T21805 arg1Of pH7.9,HEPES
R17349 T21806 T21807 arg1Of pH7.9,","
R17350 T21807 T21811 arg1Of ",",","
R17351 T21808 T21809 arg1Of 420,mM
R17352 T21810 T21807 arg2Of NaCl,","
R17353 T21810 T21808 arg1Of NaCl,420
R17354 T21811 T21815 arg1Of ",",","
R17355 T21812 T21813 arg1Of 1.5,mM
R17356 T21814 T21811 arg2Of MgCl2,","
R17357 T21814 T21812 arg1Of MgCl2,1.5
R17358 T21815 T21819 arg1Of ",",","
R17359 T21816 T21817 arg1Of 25,%
R17360 T21818 T21815 arg2Of glycerol,","
R17361 T21818 T21816 arg1Of glycerol,25
R17362 T21819 T21802 arg2Of ",",(
R17363 T21820 T21821 arg1Of 0.5,mM
R17364 T21822 T21820 arg1Of PMSF,0.5
R17365 T21822 T21823 arg1Of PMSF,","
R17366 T21823 T21827 arg1Of ",",","
R17367 T21824 T21825 arg1Of 0.2,mM
R17368 T21826 T21823 arg2Of EDTA,","
R17369 T21826 T21824 arg1Of EDTA,0.2
R17370 T21827 T21831 arg1Of ",",","
R17371 T21828 T21829 arg1Of 0.5,mM
R17372 T21830 T21827 arg2Of DTT,","
R17373 T21830 T21828 arg1Of DTT,0.5
R17374 T21831 T21819 arg2Of ",",","
R17375 T21835 T21831 arg2Of cocktail,","
R17376 T21835 T21832 arg1Of cocktail,complete
R17377 T21835 T21833 arg1Of cocktail,protease
R17378 T21835 T21834 arg1Of cocktail,inhibitor
R17379 T21836 T21802 arg3Of ),(
R17380 T21839 T21837 arg2Of °C,at
R17381 T21839 T21838 arg1Of °C,4
R17382 T21839 T21840 arg1Of °C,for
R17383 T21842 T21840 arg2Of min,for
R17384 T21842 T21841 arg1Of min,10
R17385 T21843 T21844 arg1Of Supernatants,were
R17386 T21843 T21845 arg2Of Supernatants,collected
R17387 T21845 T21844 arg2Of collected,were
R17388 T21845 T21846 arg1Of collected,as
R17389 T21845 T21849 arg1Of collected,following
R17390 T21845 T21852 arg1Of collected,at
R17391 T21848 T21846 arg2Of fractions,as
R17392 T21848 T21847 arg1Of fractions,nuclear
R17393 T21851 T21849 arg2Of centrifuge,following
R17394 T21851 T21850 arg1Of centrifuge,a
R17395 T21854 T21852 arg2Of °C,at
R17396 T21854 T21853 arg1Of °C,4
R17397 T21854 T21855 arg1Of °C,","
R17398 T21858 T21855 arg2Of g,","
R17399 T21858 T21856 arg1Of g,"13,800"
R17400 T21858 T21857 arg1Of g,×
R17401 T21858 T21859 arg1Of g,for
R17402 T21861 T21859 arg2Of min,for
R17403 T21861 T21860 arg1Of min,10

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T21863 0-11 NNP denotes Subcellular
T21864 12-25 NNP denotes Fractionation
T21865 26-37 NNP denotes Subcellular
T21866 38-51 NN denotes fractionation
T21867 52-55 VBD denotes was
T21868 56-65 VBN denotes performed
T21869 66-68 IN denotes by
T21870 69-81 JJ denotes differential
T21871 82-96 NN denotes centrifugation
T21872 97-99 IN denotes as
T21873 100-110 RB denotes previously
T21874 111-121 CD denotes described6
T21875 121-122 . denotes .
T21876 123-130 RB denotes Briefly
T21877 130-131 , denotes ,
T21878 132-137 NNS denotes cells
T21879 138-142 VBD denotes were
T21880 143-154 VBN denotes resuspended
T21881 155-157 IN denotes in
T21882 158-166 JJ denotes ice-cold
T21883 167-173 NNP denotes Buffer
T21884 174-175 NNP denotes A
T21885 176-177 -LRB- denotes (
T21886 177-179 CD denotes 10
T21887 180-182 NNP denotes mM
T21888 183-188 NNP denotes HEPES
T21889 189-191 NNP denotes pH
T21890 192-195 CD denotes 7.9
T21891 195-196 , denotes ,
T21892 197-199 CD denotes 10
T21893 200-202 NNP denotes mM
T21894 203-206 NNP denotes KCl
T21895 206-207 , denotes ,
T21896 208-211 CD denotes 1.5
T21897 212-214 NNP denotes mM
T21898 215-220 NNP denotes MgCl2
T21899 220-221 , denotes ,
T21900 222-225 CD denotes 0.1
T21901 226-228 NNP denotes mM
T21902 229-233 NNP denotes EDTA
T21903 233-234 , denotes ,
T21904 235-238 CD denotes 0.5
T21905 239-241 NNP denotes mM
T21906 242-245 NNP denotes DTT
T21907 245-246 , denotes ,
T21908 247-250 CD denotes 0.4
T21909 251-252 NN denotes %
T21910 253-258 NN denotes NP-40
T21911 258-259 , denotes ,
T21912 260-263 CD denotes 0.5
T21913 264-266 NNP denotes mM
T21914 267-271 NNP denotes PMSF
T21915 271-272 , denotes ,
T21916 273-281 JJ denotes complete
T21917 282-290 NN denotes protease
T21918 291-300 NN denotes inhibitor
T21919 301-309 NN denotes cocktail
T21920 309-310 -RRB- denotes )
T21921 311-313 IN denotes at
T21922 314-315 CD denotes 4
T21923 316-317 CD denotes °
T21924 317-318 NNP denotes C
T21925 319-322 IN denotes for
T21926 323-324 CD denotes 5
T21927 325-328 NN denotes min
T21928 328-329 . denotes .
T21929 330-337 NNS denotes Lysates
T21930 338-342 VBD denotes were
T21931 343-354 VBN denotes centrifuged
T21932 355-357 IN denotes at
T21933 358-359 CD denotes 4
T21934 360-361 CD denotes °
T21935 361-362 NNP denotes C
T21936 362-363 , denotes ,
T21937 364-367 CD denotes 500
T21938 368-369 NN denotes ×
T21939 370-371 NN denotes g
T21940 372-375 IN denotes for
T21941 376-377 CD denotes 3
T21942 378-381 NN denotes min
T21943 381-382 , denotes ,
T21944 383-386 CC denotes and
T21945 387-399 NNS denotes supernatants
T21946 400-404 VBD denotes were
T21947 405-414 VBN denotes collected
T21948 415-417 RB denotes as
T21949 418-427 JJ denotes cytosolic
T21950 428-437 NNS denotes fractions
T21951 437-438 . denotes .
T21952 439-446 NNS denotes Pellets
T21953 447-451 VBD denotes were
T21954 452-461 VBN denotes incubated
T21955 462-464 IN denotes in
T21956 465-471 NNP denotes Buffer
T21957 472-473 NNP denotes C
T21958 474-475 -LRB- denotes (
T21959 475-477 CD denotes 20
T21960 478-480 NNP denotes mM
T21961 481-486 NNP denotes HEPES
T21962 487-490 NNP denotes pH7
T21963 490-492 CD denotes .9
T21964 492-493 , denotes ,
T21965 494-497 CD denotes 420
T21966 498-500 NNP denotes mM
T21967 501-505 NNP denotes NaCl
T21968 505-506 , denotes ,
T21969 507-510 CD denotes 1.5
T21970 511-513 NNP denotes mM
T21971 514-519 NNP denotes MgCl2
T21972 519-520 , denotes ,
T21973 521-523 CD denotes 25
T21974 523-524 NN denotes %
T21975 525-533 NN denotes glycerol
T21976 533-534 , denotes ,
T21977 535-538 CD denotes 0.5
T21978 539-541 NNP denotes mM
T21979 542-546 NNP denotes PMSF
T21980 546-547 , denotes ,
T21981 548-551 CD denotes 0.2
T21982 552-554 NNP denotes mM
T21983 555-559 NNP denotes EDTA
T21984 559-560 , denotes ,
T21985 561-564 CD denotes 0.5
T21986 565-567 NNP denotes mM
T21987 568-571 NNP denotes DTT
T21988 571-572 , denotes ,
T21989 573-581 JJ denotes complete
T21990 582-590 NN denotes protease
T21991 591-600 NN denotes inhibitor
T21992 601-609 NN denotes cocktail
T21993 609-610 -RRB- denotes )
T21994 611-613 IN denotes at
T21995 614-615 CD denotes 4
T21996 616-617 CD denotes °
T21997 617-618 NNP denotes C
T21998 619-622 IN denotes for
T21999 623-625 CD denotes 10
T22000 626-629 NN denotes min
T22001 629-630 . denotes .
T22002 631-643 NNS denotes Supernatants
T22003 644-648 VBD denotes were
T22004 649-658 VBN denotes collected
T22005 659-661 IN denotes as
T22006 662-669 JJ denotes nuclear
T22007 670-679 NNS denotes fractions
T22008 680-689 VBG denotes following
T22009 690-691 DT denotes a
T22010 692-702 NN denotes centrifuge
T22011 703-705 IN denotes at
T22012 706-707 CD denotes 4
T22013 708-709 CD denotes °
T22014 709-710 NNP denotes C
T22015 710-711 , denotes ,
T22016 712-718 CD denotes 13,800
T22017 719-720 NN denotes ×
T22018 721-722 NN denotes g
T22019 723-726 IN denotes for
T22020 727-729 CD denotes 10
T22021 730-733 NN denotes min
T22022 733-734 . denotes .
R17404 T21863 T21866 compound Subcellular,fractionation
R17405 T21864 T21866 compound Fractionation,fractionation
R17406 T21865 T21866 compound Subcellular,fractionation
R17407 T21866 T21868 nsubjpass fractionation,performed
R17408 T21867 T21868 auxpass was,performed
R17409 T21868 T21868 ROOT performed,performed
R17410 T21869 T21868 agent by,performed
R17411 T21870 T21871 compound differential,centrifugation
R17412 T21871 T21869 pobj centrifugation,by
R17413 T21872 T21868 prep as,performed
R17414 T21873 T21874 advmod previously,described6
R17415 T21874 T21872 pobj described6,as
R17416 T21875 T21868 punct .,performed
R17417 T21876 T21880 advmod Briefly,resuspended
R17418 T21877 T21880 punct ",",resuspended
R17419 T21878 T21880 nsubjpass cells,resuspended
R17420 T21879 T21880 auxpass were,resuspended
R17421 T21880 T21880 ROOT resuspended,resuspended
R17422 T21881 T21880 prep in,resuspended
R17423 T21882 T21884 amod ice-cold,A
R17424 T21883 T21884 compound Buffer,A
R17425 T21884 T21881 pobj A,in
R17426 T21885 T21884 punct (,A
R17427 T21886 T21889 nummod 10,pH
R17428 T21887 T21889 compound mM,pH
R17429 T21888 T21889 compound HEPES,pH
R17430 T21889 T21884 appos pH,A
R17431 T21890 T21889 nummod 7.9,pH
R17432 T21891 T21889 punct ",",pH
R17433 T21892 T21894 nummod 10,KCl
R17434 T21893 T21894 compound mM,KCl
R17435 T21894 T21889 appos KCl,pH
R17436 T21895 T21894 punct ",",KCl
R17437 T21896 T21898 nummod 1.5,MgCl2
R17438 T21897 T21898 compound mM,MgCl2
R17439 T21898 T21894 appos MgCl2,KCl
R17440 T21899 T21898 punct ",",MgCl2
R17441 T21900 T21902 nummod 0.1,EDTA
R17442 T21901 T21902 compound mM,EDTA
R17443 T21902 T21898 appos EDTA,MgCl2
R17444 T21903 T21902 punct ",",EDTA
R17445 T21904 T21906 nummod 0.5,DTT
R17446 T21905 T21906 compound mM,DTT
R17447 T21906 T21902 appos DTT,EDTA
R17448 T21907 T21906 punct ",",DTT
R17449 T21908 T21909 nummod 0.4,%
R17450 T21909 T21910 compound %,NP-40
R17451 T21910 T21906 appos NP-40,DTT
R17452 T21911 T21910 punct ",",NP-40
R17453 T21912 T21914 nummod 0.5,PMSF
R17454 T21913 T21914 compound mM,PMSF
R17455 T21914 T21910 appos PMSF,NP-40
R17456 T21915 T21914 punct ",",PMSF
R17457 T21916 T21919 amod complete,cocktail
R17458 T21917 T21919 compound protease,cocktail
R17459 T21918 T21919 compound inhibitor,cocktail
R17460 T21919 T21914 appos cocktail,PMSF
R17461 T21920 T21914 punct ),PMSF
R17462 T21921 T21906 prep at,DTT
R17463 T21922 T21923 nummod 4,°
R17464 T21923 T21921 pobj °,at
R17465 T21924 T21906 conj C,DTT
R17466 T21925 T21906 prep for,DTT
R17467 T21926 T21927 nummod 5,min
R17468 T21927 T21925 pobj min,for
R17469 T21928 T21880 punct .,resuspended
R17470 T21929 T21931 nsubjpass Lysates,centrifuged
R17471 T21930 T21931 auxpass were,centrifuged
R17472 T21931 T21931 ROOT centrifuged,centrifuged
R17473 T21932 T21931 prep at,centrifuged
R17474 T21933 T21934 nummod 4,°
R17475 T21934 T21935 nummod °,C
R17476 T21935 T21932 pobj C,at
R17477 T21936 T21935 punct ",",C
R17478 T21937 T21938 nummod 500,×
R17479 T21938 T21939 compound ×,g
R17480 T21939 T21947 nsubjpass g,collected
R17481 T21940 T21939 prep for,g
R17482 T21941 T21942 nummod 3,min
R17483 T21942 T21940 pobj min,for
R17484 T21943 T21939 punct ",",g
R17485 T21944 T21939 cc and,g
R17486 T21945 T21939 conj supernatants,g
R17487 T21946 T21947 auxpass were,collected
R17488 T21947 T21931 conj collected,centrifuged
R17489 T21948 T21947 prep as,collected
R17490 T21949 T21950 amod cytosolic,fractions
R17491 T21950 T21948 pobj fractions,as
R17492 T21951 T21947 punct .,collected
R17493 T21952 T21954 nsubjpass Pellets,incubated
R17494 T21953 T21954 auxpass were,incubated
R17495 T21954 T21954 ROOT incubated,incubated
R17496 T21955 T21954 prep in,incubated
R17497 T21956 T21957 compound Buffer,C
R17498 T21957 T21955 pobj C,in
R17499 T21958 T21957 punct (,C
R17500 T21959 T21962 nummod 20,pH7
R17501 T21960 T21962 compound mM,pH7
R17502 T21961 T21962 compound HEPES,pH7
R17503 T21962 T21957 appos pH7,C
R17504 T21963 T21962 nummod .9,pH7
R17505 T21964 T21962 punct ",",pH7
R17506 T21965 T21967 nummod 420,NaCl
R17507 T21966 T21967 compound mM,NaCl
R17508 T21967 T21962 appos NaCl,pH7
R17509 T21968 T21967 punct ",",NaCl
R17510 T21969 T21971 nummod 1.5,MgCl2
R17511 T21970 T21971 compound mM,MgCl2
R17512 T21971 T21967 appos MgCl2,NaCl
R17513 T21972 T21971 punct ",",MgCl2
R17514 T21973 T21974 nummod 25,%
R17515 T21974 T21975 compound %,glycerol
R17516 T21975 T21971 appos glycerol,MgCl2
R17517 T21976 T21971 punct ",",MgCl2
R17518 T21977 T21979 nummod 0.5,PMSF
R17519 T21978 T21979 compound mM,PMSF
R17520 T21979 T21971 appos PMSF,MgCl2
R17521 T21980 T21979 punct ",",PMSF
R17522 T21981 T21983 nummod 0.2,EDTA
R17523 T21982 T21983 compound mM,EDTA
R17524 T21983 T21979 appos EDTA,PMSF
R17525 T21984 T21983 punct ",",EDTA
R17526 T21985 T21987 nummod 0.5,DTT
R17527 T21986 T21987 compound mM,DTT
R17528 T21987 T21983 appos DTT,EDTA
R17529 T21988 T21987 punct ",",DTT
R17530 T21989 T21992 amod complete,cocktail
R17531 T21990 T21992 compound protease,cocktail
R17532 T21991 T21992 compound inhibitor,cocktail
R17533 T21992 T21987 appos cocktail,DTT
R17534 T21993 T21987 punct ),DTT
R17535 T21994 T21979 prep at,PMSF
R17536 T21995 T21996 nummod 4,°
R17537 T21996 T21994 pobj °,at
R17538 T21997 T21979 conj C,PMSF
R17539 T21998 T21979 prep for,PMSF
R17540 T21999 T22000 nummod 10,min
R17541 T22000 T21998 pobj min,for
R17542 T22001 T21954 punct .,incubated
R17543 T22002 T22004 nsubjpass Supernatants,collected
R17544 T22003 T22004 auxpass were,collected
R17545 T22004 T22004 ROOT collected,collected
R17546 T22005 T22004 prep as,collected
R17547 T22006 T22007 amod nuclear,fractions
R17548 T22007 T22005 pobj fractions,as
R17549 T22008 T22004 prep following,collected
R17550 T22009 T22010 det a,centrifuge
R17551 T22010 T22008 pobj centrifuge,following
R17552 T22011 T22010 prep at,centrifuge
R17553 T22012 T22013 nummod 4,°
R17554 T22013 T22011 pobj °,at
R17555 T22014 T22018 nmod C,g
R17556 T22015 T22014 punct ",",C
R17557 T22016 T22017 nummod "13,800",×
R17558 T22017 T22018 compound ×,g
R17559 T22018 T22010 npadvmod g,centrifuge
R17560 T22019 T22018 prep for,g
R17561 T22020 T22021 nummod 10,min
R17562 T22021 T22019 pobj min,for
R17563 T22022 T22004 punct .,collected

GO-CC

Id Subject Object Predicate Lexical cue
T22029 132-137 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T21706 0-25 Sentence denotes Subcellular Fractionation
T21707 26-122 Sentence denotes Subcellular fractionation was performed by differential centrifugation as previously described6.
T21708 123-329 Sentence denotes Briefly, cells were resuspended in ice-cold Buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1 mM EDTA, 0.5 mM DTT, 0.4 % NP-40, 0.5 mM PMSF, complete protease inhibitor cocktail) at 4 °C for 5 min.
T21709 330-438 Sentence denotes Lysates were centrifuged at 4 °C, 500 × g for 3 min, and supernatants were collected as cytosolic fractions.
T21710 439-630 Sentence denotes Pellets were incubated in Buffer C (20 mM HEPES pH7.9, 420 mM NaCl, 1.5 mM MgCl2, 25% glycerol, 0.5 mM PMSF, 0.2 mM EDTA, 0.5 mM DTT, complete protease inhibitor cocktail) at 4 °C for 10 min.
T21711 631-734 Sentence denotes Supernatants were collected as nuclear fractions following a centrifuge at 4 °C, 13,800 × g for 10 min.
T220 0-25 Sentence denotes Subcellular Fractionation
T221 26-122 Sentence denotes Subcellular fractionation was performed by differential centrifugation as previously described6.
T222 123-329 Sentence denotes Briefly, cells were resuspended in ice-cold Buffer A (10 mM HEPES pH 7.9, 10 mM KCl, 1.5 mM MgCl2, 0.1 mM EDTA, 0.5 mM DTT, 0.4 % NP-40, 0.5 mM PMSF, complete protease inhibitor cocktail) at 4 °C for 5 min.
T223 330-438 Sentence denotes Lysates were centrifuged at 4 °C, 500 × g for 3 min, and supernatants were collected as cytosolic fractions.
T224 439-630 Sentence denotes Pellets were incubated in Buffer C (20 mM HEPES pH7.9, 420 mM NaCl, 1.5 mM MgCl2, 25% glycerol, 0.5 mM PMSF, 0.2 mM EDTA, 0.5 mM DTT, complete protease inhibitor cocktail) at 4 °C for 10 min.
T225 631-734 Sentence denotes Supernatants were collected as nuclear fractions following a centrifuge at 4 °C, 13,800 × g for 10 min.