PMC:2817507 / 7334-7749 JSONTXT

{"project":"2_test","denotations":[{"id":"20150961-17884182-67933706","span":{"begin":97,"end":98},"obj":"17884182"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"pmc-enju-pas","denotations":[{"id":"T3605","span":{"begin":406,"end":414},"obj":"NN"},{"id":"T3604","span":{"begin":397,"end":405},"obj":"JJ"},{"id":"T3603","span":{"begin":394,"end":396},"obj":"IN"},{"id":"T3602","span":{"begin":387,"end":393},"obj":"VB"},{"id":"T3601","span":{"begin":376,"end":386},"obj":"NN"},{"id":"T3600","span":{"begin":368,"end":375},"obj":"JJ"},{"id":"T3599","span":{"begin":360,"end":367},"obj":"IN"},{"id":"T3598","span":{"begin":350,"end":359},"obj":"VB"},{"id":"T3597","span":{"begin":343,"end":349},"obj":"NN"},{"id":"T3596","span":{"begin":340,"end":341},"obj":"-RRB-"},{"id":"T3595","span":{"begin":338,"end":340},"obj":"NNP"},{"id":"T3594","span":{"begin":336,"end":337},"obj":"-COMMA-"},{"id":"T3593","span":{"begin":332,"end":336},"obj":"NNP"},{"id":"T3592","span":{"begin":326,"end":331},"obj":"NNP"},{"id":"T3591","span":{"begin":325,"end":326},"obj":"-LRB-"},{"id":"T3590","span":{"begin":320,"end":324},"obj":"NNP"},{"id":"T3589","span":{"begin":306,"end":319},"obj":"NNP"},{"id":"T3588","span":{"begin":301,"end":305},"obj":"NNP"},{"id":"T3587","span":{"begin":295,"end":300},"obj":"NNP"},{"id":"T3586","span":{"begin":290,"end":294},"obj":"IN"},{"id":"T3585","span":{"begin":281,"end":289},"obj":"VB"},{"id":"T3584","span":{"begin":276,"end":280},"obj":"VB"},{"id":"T3583","span":{"begin":274,"end":275},"obj":"-RRB-"},{"id":"T3582","span":{"begin":267,"end":274},"obj":"NN"},{"id":"T3581","span":{"begin":265,"end":266},"obj":"-COMMA-"},{"id":"T3580","span":{"begin":258,"end":265},"obj":"NN"},{"id":"T3579","span":{"begin":254,"end":257},"obj":"NN"},{"id":"T3578","span":{"begin":253,"end":254},"obj":"-LRB-"},{"id":"T3577","span":{"begin":243,"end":252},"obj":"NN"},{"id":"T3576","span":{"begin":209,"end":242},"obj":"JJ"},{"id":"T3575","span":{"begin":205,"end":208},"obj":"CC"},{"id":"T3574","span":{"begin":196,"end":204},"obj":"NN"},{"id":"T3573","span":{"begin":186,"end":195},"obj":"JJ"},{"id":"T3572","span":{"begin":173,"end":185},"obj":"VB"},{"id":"T3571","span":{"begin":169,"end":172},"obj":"DT"},{"id":"T3570","span":{"begin":166,"end":167},"obj":"-RRB-"},{"id":"T3569","span":{"begin":160,"end":166},"obj":"NN"},{"id":"T3568","span":{"begin":158,"end":159},"obj":"-COMMA-"},{"id":"T3567","span":{"begin":153,"end":158},"obj":"NNP"},{"id":"T3566","span":{"begin":152,"end":153},"obj":"-LRB-"},{"id":"T3565","span":{"begin":142,"end":151},"obj":"NN"},{"id":"T3564","span":{"begin":135,"end":141},"obj":"NN"},{"id":"T3563","span":{"begin":131,"end":134},"obj":"VB"},{"id":"T3562","span":{"begin":122,"end":130},"obj":"VB"},{"id":"T3561","span":{"begin":113,"end":121},"obj":"NN"},{"id":"T3560","span":{"begin":105,"end":112},"obj":"JJ"},{"id":"T3559","span":{"begin":101,"end":104},"obj":"DT"},{"id":"T3558","span":{"begin":98,"end":99},"obj":"-RRB-"},{"id":"T3557","span":{"begin":97,"end":98},"obj":"CD"},{"id":"T3556","span":{"begin":96,"end":97},"obj":"-LRB-"},{"id":"T3555","span":{"begin":85,"end":95},"obj":"RB"},{"id":"T3554","span":{"begin":75,"end":84},"obj":"VB"},{"id":"T3553","span":{"begin":72,"end":74},"obj":"IN"},{"id":"T3552","span":{"begin":62,"end":71},"obj":"VB"},{"id":"T3551","span":{"begin":58,"end":61},"obj":"VB"},{"id":"T3550","span":{"begin":53,"end":57},"obj":"NN"},{"id":"T3549","span":{"begin":49,"end":52},"obj":"IN"},{"id":"T3548","span":{"begin":40,"end":48},"obj":"NN"},{"id":"T3547","span":{"begin":36,"end":39},"obj":"NN"},{"id":"T3546","span":{"begin":32,"end":35},"obj":"DT"},{"id":"T3545","span":{"begin":30,"end":31},"obj":"-RRB-"},{"id":"T3544","span":{"begin":27,"end":30},"obj":"NN"},{"id":"T3543","span":{"begin":26,"end":27},"obj":"-LRB-"},{"id":"T3542","span":{"begin":5,"end":25},"obj":"NN"},{"id":"T3541","span":{"begin":0,"end":3},"obj":"CD"}],"relations":[{"id":"R2754","pred":"arg1Of","subj":"T3542","obj":"T3543"},{"id":"R2755","pred":"arg2Of","subj":"T3544","obj":"T3543"},{"id":"R2756","pred":"arg3Of","subj":"T3545","obj":"T3543"},{"id":"R2757","pred":"arg1Of","subj":"T3548","obj":"T3546"},{"id":"R2758","pred":"arg1Of","subj":"T3548","obj":"T3547"},{"id":"R2759","pred":"arg1Of","subj":"T3548","obj":"T3549"},{"id":"R2760","pred":"arg1Of","subj":"T3548","obj":"T3551"},{"id":"R2761","pred":"arg2Of","subj":"T3548","obj":"T3552"},{"id":"R2762","pred":"arg2Of","subj":"T3550","obj":"T3549"},{"id":"R2763","pred":"arg2Of","subj":"T3552","obj":"T3551"},{"id":"R2764","pred":"arg1Of","subj":"T3552","obj":"T3553"},{"id":"R2765","pred":"arg1Of","subj":"T3552","obj":"T3556"},{"id":"R2766","pred":"arg2Of","subj":"T3554","obj":"T3553"},{"id":"R2767","pred":"arg1Of","subj":"T3554","obj":"T3555"},{"id":"R2768","pred":"arg2Of","subj":"T3557","obj":"T3556"},{"id":"R2769","pred":"arg3Of","subj":"T3558","obj":"T3556"},{"id":"R2770","pred":"arg1Of","subj":"T3561","obj":"T3559"},{"id":"R2771","pred":"arg1Of","subj":"T3561","obj":"T3560"},{"id":"R2772","pred":"arg2Of","subj":"T3561","obj":"T3562"},{"id":"R2773","pred":"arg1Of","subj":"T3561","obj":"T3563"},{"id":"R2774","pred":"arg1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Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"bionlp-st-ge-2016-test-proteins","denotations":[{"id":"T3521","span":{"begin":225,"end":231},"obj":"Protein"},{"id":"T3520","span":{"begin":53,"end":57},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T3676","span":{"begin":53,"end":57},"obj":"http://www.uniprot.org/uniprot/O00206"},{"id":"T3677","span":{"begin":147,"end":151},"obj":"http://www.uniprot.org/uniprot/O00206"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"GO-BP","denotations":[{"id":"T3522","span":{"begin":338,"end":340},"obj":"http://purl.obolibrary.org/obo/GO_0033968"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"GO-MF","denotations":[{"id":"T3527","span":{"begin":338,"end":340},"obj":"http://purl.obolibrary.org/obo/GO_0033968"},{"id":"T3526","span":{"begin":376,"end":386},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T3525","span":{"begin":209,"end":217},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T3524","span":{"begin":196,"end":204},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T3523","span":{"begin":113,"end":121},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"GO-CC","denotations":[{"id":"T3528","span":{"begin":113,"end":121},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T3535","span":{"begin":376,"end":386},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T3534","span":{"begin":209,"end":217},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T3533","span":{"begin":196,"end":204},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T3532","span":{"begin":113,"end":121},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T3531","span":{"begin":376,"end":386},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T3530","span":{"begin":209,"end":217},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T3529","span":{"begin":196,"end":204},"obj":"http://purl.obolibrary.org/obo/GO_0019815"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"sentences","denotations":[{"id":"T3517","span":{"begin":343,"end":415},"obj":"Sentence"},{"id":"T3516","span":{"begin":169,"end":342},"obj":"Sentence"},{"id":"T3515","span":{"begin":101,"end":168},"obj":"Sentence"},{"id":"T3514","span":{"begin":32,"end":100},"obj":"Sentence"},{"id":"T3513","span":{"begin":5,"end":31},"obj":"Sentence"},{"id":"T3512","span":{"begin":0,"end":4},"obj":"Sentence"},{"id":"T71","span":{"begin":0,"end":4},"obj":"Sentence"},{"id":"T72","span":{"begin":5,"end":31},"obj":"Sentence"},{"id":"T73","span":{"begin":32,"end":100},"obj":"Sentence"},{"id":"T74","span":{"begin":101,"end":168},"obj":"Sentence"},{"id":"T75","span":{"begin":169,"end":342},"obj":"Sentence"},{"id":"T76","span":{"begin":343,"end":415},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"simple1","denotations":[{"id":"T3540","span":{"begin":225,"end":231},"obj":"Protein"},{"id":"T3539","span":{"begin":53,"end":57},"obj":"Protein"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"BioNLP16_DUT","denotations":[{"id":"T3690","span":{"begin":225,"end":231},"obj":"Protein"},{"id":"T3689","span":{"begin":53,"end":57},"obj":"Protein"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"BioNLP16_Messiy","denotations":[{"id":"T3679","span":{"begin":225,"end":231},"obj":"Protein"},{"id":"T3678","span":{"begin":53,"end":57},"obj":"Protein"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"DLUT931","denotations":[{"id":"T3685","span":{"begin":225,"end":231},"obj":"Protein"},{"id":"T3684","span":{"begin":53,"end":57},"obj":"Protein"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

{"project":"bionlp-st-ge-2016-test-ihmc","denotations":[{"id":"T3704","span":{"begin":169,"end":204},"obj":"Protein_modification"},{"id":"T3703","span":{"begin":254,"end":275},"obj":"Entity"},{"id":"T3702","span":{"begin":232,"end":242},"obj":"Protein"},{"id":"T3701","span":{"begin":368,"end":386},"obj":"Entity"},{"id":"T3700","span":{"begin":53,"end":57},"obj":"Protein"},{"id":"T3699","span":{"begin":101,"end":130},"obj":"Protein"},{"id":"T3698","span":{"begin":135,"end":167},"obj":"Protein"},{"id":"T3697","span":{"begin":209,"end":252},"obj":"Entity"},{"id":"T3696","span":{"begin":169,"end":204},"obj":"Protein"},{"id":"T3695","span":{"begin":343,"end":414},"obj":"Entity"},{"id":"T3694","span":{"begin":218,"end":224},"obj":"Entity"},{"id":"T3693","span":{"begin":225,"end":231},"obj":"Protein"},{"id":"T3692","span":{"begin":295,"end":300},"obj":"Protein"},{"id":"T3691","span":{"begin":209,"end":217},"obj":"Entity"}],"relations":[{"id":"R2885","pred":"themeOf","subj":"T3696","obj":"T3704"}],"text":"2.4. Immunohistochemistry (IHC)\nThe IHC staining for TLR4 was performed as described previously [8]. The primary antibody employed was rabbit anti-TLR4 (WuHan, BA1717). The biotinylated secondary antibody and antibody-biotin-avidin-peroxidase complexes (ABC reagent, SC-2018) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Slides processed without primary antibodies served as negative controls."}

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