PMC:2817507 / 5816-7332 JSONTXT

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The house keep gene (β-actin) was used as an internal control. The PCR primer sequences were designed according to the TLR4 and β-actin gene sequences reported in GenBank. TLR4 (145 bp): forward: 5′-AGCTTTGGTCAGTTGGCTCT-3′; reverse: 5′-CAGGATGACACCATTGAAGC-3′; β-actin (701 bp): forward: 5′-GCCAACCGTGAAAAAGATG-3′; reverse: 5′-CCAGGATAGAGCCACCAAT-3′. Reaction cycles were performed at the following condition: 94°C 2 min, 94°C 40 s, 55°C 40 s, and 72°C 60 s. The reaction was stopped after 31 cycles. PCR products were electrophoresed through agarose gel. Image Acquisition and Analysis software were used to determine band densities. 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