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PMC:2817507 / 5816-7332 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T3068 1511-1515 NN denotes mRNA
T3067 1503-1510 NN denotes β-actin
T3066 1500-1502 TO denotes to
T3065 1495-1499 NN denotes mRNA
T3064 1490-1494 NN denotes TLR4
T3063 1487-1489 IN denotes of
T3062 1481-1486 NN denotes ratio
T3061 1477-1480 DT denotes the
T3060 1474-1476 IN denotes as
T3059 1464-1473 VB denotes expressed
T3058 1460-1463 VB denotes was
T3057 1455-1459 NN denotes mRNA
T3056 1450-1454 NN denotes TLR4
T3055 1447-1449 IN denotes of
T3054 1437-1446 NN denotes abundance
T3053 1433-1436 DT denotes The
T3052 1422-1431 NN denotes densities
T3051 1417-1421 NN denotes band
T3050 1407-1416 VB denotes determine
T3049 1404-1406 TO denotes to
T3048 1399-1403 VB denotes used
T3047 1394-1398 VB denotes were
T3046 1385-1393 NN denotes software
T3045 1376-1384 NN denotes Analysis
T3044 1372-1375 CC denotes and
T3043 1360-1371 NNP denotes Acquisition
T3042 1354-1359 NN denotes Image
T3041 1349-1352 NN denotes gel
T3040 1341-1348 NN denotes agarose
T3039 1333-1340 IN denotes through
T3038 1317-1332 VB denotes electrophoresed
T3037 1312-1316 VB denotes were
T3036 1303-1311 NN denotes products
T3035 1299-1302 NN denotes PCR
T3034 1291-1297 NN denotes cycles
T3033 1288-1290 CD denotes 31
T3032 1282-1287 IN denotes after
T3031 1274-1281 VB denotes stopped
T3030 1270-1273 VB denotes was
T3029 1261-1269 NN denotes reaction
T3028 1257-1260 DT denotes The
T3027 1251-1255 NN denotes 60 s
T3026 1246-1250 NN denotes 72°C
T3025 1242-1245 CC denotes and
T3024 1240-1241 -COMMA- denotes ,
T3023 1236-1240 NN denotes 40 s
T3022 1231-1235 NN denotes 55°C
T3021 1229-1230 -COMMA- denotes ,
T3020 1225-1229 NN denotes 40 s
T3019 1220-1224 NN denotes 94°C
T3018 1218-1219 -COMMA- denotes ,
T3017 1213-1218 NN denotes 2 min
T3016 1208-1212 NN denotes 94°C
T3015 1206-1207 -COLON- denotes :
T3014 1197-1206 NN denotes condition
T3013 1187-1196 VB denotes following
T3012 1183-1186 DT denotes the
T3011 1180-1182 IN denotes at
T3010 1170-1179 VB denotes performed
T3009 1165-1169 VB denotes were
T3008 1158-1164 NN denotes cycles
T3007 1149-1157 NN denotes Reaction
T3006 1122-1147 NN denotes 5′-CCAGGATAGAGCCACCAAT-3′
T3005 1120-1121 -COLON- denotes :
T3004 1113-1120 NN denotes reverse
T3003 1111-1112 -COLON- denotes ;
T3002 1086-1111 NN denotes 5′-GCCAACCGTGAAAAAGATG-3′
T3001 1084-1085 -COLON- denotes :
T3000 1077-1084 RB denotes forward
T2999 1075-1076 -COLON- denotes :
T2998 1074-1075 -RRB- denotes )
T2997 1068-1074 NN denotes 701 bp
T2996 1067-1068 -LRB- denotes (
T2995 1059-1066 NN denotes β-actin
T2994 1057-1058 -COLON- denotes ;
T2993 1031-1057 NN denotes 5′-CAGGATGACACCATTGAAGC-3′
T2992 1029-1030 -COLON- denotes :
T2991 1022-1029 NN denotes reverse
T2990 1020-1021 -COLON- denotes ;
T2989 994-1020 NN denotes 5′-AGCTTTGGTCAGTTGGCTCT-3′
T2988 992-993 -COLON- denotes :
T2987 985-992 RB denotes forward
T2986 983-984 -COLON- denotes :
T2985 982-983 -RRB- denotes )
T2984 976-982 NN denotes 145 bp
T2983 975-976 -LRB- denotes (
T2982 970-974 NN denotes TLR4
T2981 961-968 NNP denotes GenBank
T2980 958-960 IN denotes in
T2979 949-957 VB denotes reported
T2978 939-948 NN denotes sequences
T2977 934-938 NN denotes gene
T2976 926-933 JJ denotes β-actin
T2975 922-925 CC denotes and
T2974 917-921 NN denotes TLR4
T2973 913-916 DT denotes the
T2972 910-912 TO denotes to
T2971 900-909 VB denotes according
T2970 891-899 VB denotes designed
T2969 886-890 VB denotes were
T2968 876-885 NN denotes sequences
T2967 869-875 NN denotes primer
T2966 865-868 NN denotes PCR
T2965 861-864 DT denotes The
T2964 852-859 NN denotes control
T2963 843-851 JJ denotes internal
T2962 840-842 DT denotes an
T2961 837-839 IN denotes as
T2960 832-836 VB denotes used
T2959 828-831 VB denotes was
T2958 826-827 -RRB- denotes )
T2957 819-826 NN denotes β-actin
T2956 818-819 -LRB- denotes (
T2955 813-817 NN denotes gene
T2954 808-812 NN denotes keep
T2953 802-807 NN denotes house
T2952 798-801 DT denotes The
T2951 786-796 NN denotes polymerase
T2950 782-785 NN denotes DNA
T2949 778-781 NN denotes Taq
T2948 775-777 NN denotes 2u
T2947 771-774 CC denotes and
T2946 769-770 -COMMA- denotes ,
T2945 762-769 NN denotes primers
T2944 759-761 IN denotes of
T2943 752-758 NN denotes 200 μM
T2942 750-751 -COMMA- denotes ,
T2941 746-750 NN denotes dNTP
T2940 741-745 DT denotes each
T2939 738-740 IN denotes of
T2938 731-737 NN denotes 200 μM
T2937 729-730 -COMMA- denotes ,
T2936 717-729 NN denotes 1.5 mM MgCl2
T2935 715-716 -COMMA- denotes ,
T2934 709-715 NN denotes buffer
T2933 705-708 NN denotes PCR
T2932 702-704 NN denotes 1×
T2931 697-701 IN denotes with
T2930 688-696 NN denotes fragment
T2929 683-687 NN denotes gene
T2928 678-682 NN denotes TLR4
T2927 674-677 DT denotes the
T2926 666-673 VB denotes amplify
T2925 663-665 TO denotes to
T2924 658-662 VB denotes used
T2923 654-657 VB denotes was
T2922 649-653 NN denotes cDNA
T2921 646-648 IN denotes of
T2920 635-645 NN denotes microliter
T2919 631-634 CD denotes One
T2918 621-629 NN denotes supplier
T2917 617-620 DT denotes the
T2916 614-616 IN denotes by
T2915 604-613 VB denotes described
T2914 593-603 NN denotes conditions
T2913 587-592 IN denotes under
T2912 585-586 -RRB- denotes )
T2911 582-585 NNP denotes Inc
T2910 574-581 JJ denotes promega
T2909 573-574 -LRB- denotes (
T2908 566-572 NN denotes RT-PCR
T2907 562-565 IN denotes for
T2906 558-561 NN denotes Kit
T2905 548-557 NNP denotes Synthesis
T2904 535-547 NNP denotes First-Strand
T2903 523-534 NNP denotes Superscript
T2902 517-522 VB denotes using
T2901 510-516 NN denotes system
T2900 501-509 NN denotes reaction
T2899 495-500 NN denotes 20 μL
T2898 492-494 IN denotes in
T2897 487-491 NN denotes cDNA
T2896 482-486 IN denotes into
T2895 462-481 VB denotes reverse-transcribed
T2894 458-461 VB denotes was
T2893 454-457 NN denotes RNA
T2892 444-452 NN denotes 1.0 g/mL
T2891 441-443 TO denotes to
T2890 432-440 VB denotes adjusted
T2889 427-431 VB denotes were
T2888 422-426 NN denotes mRNA
T2887 419-421 IN denotes of
T2886 404-418 NN denotes concentrations
T2885 400-403 DT denotes the
T2884 398-399 -COMMA- denotes ,
T2883 394-398 RB denotes Then
T2882 382-392 NN denotes wavelength
T2881 374-381 NN denotes A280 nm
T2880 370-373 CC denotes and
T2879 365-369 NN denotes A260
T2878 362-364 IN denotes at
T2877 354-361 NN denotes density
T2876 346-353 JJ denotes optical
T2875 336-345 VB denotes measuring
T2874 333-335 IN denotes by
T2873 331-332 -RRB- denotes )
T2872 314-331 NN denotes Spectrophotometer
T2871 306-313 NN denotes ND-1000
T2870 305-306 -LRB- denotes (
T2869 296-304 NNP denotes Nanodrop
T2868 294-295 DT denotes a
T2867 291-293 IN denotes on
T2866 280-290 VB denotes determined
T2865 276-279 VB denotes was
T2864 272-275 NN denotes RNA
T2863 266-271 JJ denotes total
T2862 263-265 IN denotes of
T2861 254-262 NN denotes quantity
T2860 250-253 DT denotes The
T2859 236-248 NN denotes instructions
T2858 233-235 POS denotes 's
T2857 221-233 NN denotes manufacturer
T2856 217-220 DT denotes the
T2855 214-216 TO denotes to
T2854 204-213 VB denotes according
T2853 202-203 -RRB- denotes )
T2852 197-202 NNP denotes China
T2851 195-196 -COMMA- denotes ,
T2850 193-195 NNP denotes HZ
T2849 191-192 -COMMA- denotes ,
T2848 184-191 NNP denotes BioFlux
T2847 183-184 -LRB- denotes (
T2846 179-182 NN denotes Kit
T2845 169-178 NN denotes isolation
T2844 165-168 NN denotes RNA
T2843 159-164 NNP denotes Total
T2842 157-158 NNP denotes P
T2841 150-156 NNP denotes Simply
T2840 144-149 VB denotes using
T2839 136-143 NN denotes ganglia
T2838 130-135 JJ denotes basal
T2837 125-129 JJ denotes left
T2836 122-124 IN denotes in
T2835 114-121 NN denotes regions
T2834 98-113 JJ denotes peri-hemorrhage
T2833 93-97 IN denotes from
T2832 84-92 VB denotes isolated
T2831 79-83 VB denotes were
T2830 71-78 NN denotes samples
T2829 67-70 NN denotes RNA
T2828 61-66 JJ denotes total
T2827 59-60 -COMMA- denotes ,
T2826 52-59 RB denotes Briefly
T2825 45-51 NN denotes RT-PCR
T2824 42-44 IN denotes of
T2823 35-41 NN denotes Method
T2822 31-34 DT denotes the
T2821 28-30 IN denotes by
T2820 23-27 NN denotes mRNA
T2819 18-22 NN denotes TLR4
T2818 15-17 IN denotes of
T2817 5-14 NN denotes Detection
T2816 0-3 CD denotes 2.3
R2188 T2817 T2818 arg1Of Detection,of
R2189 T2817 T2821 arg1Of Detection,by
R2190 T2820 T2818 arg2Of mRNA,of
R2191 T2820 T2819 arg1Of mRNA,TLR4
R2192 T2823 T2821 arg2Of Method,by
R2193 T2823 T2822 arg1Of Method,the
R2194 T2823 T2824 arg1Of Method,of
R2195 T2825 T2824 arg2Of RT-PCR,of
R2196 T2830 T2828 arg1Of samples,total
R2197 T2830 T2829 arg1Of samples,RNA
R2198 T2830 T2831 arg1Of samples,were
R2199 T2830 T2832 arg2Of samples,isolated
R2200 T2832 T2826 arg1Of isolated,Briefly
R2201 T2832 T2827 arg1Of isolated,","
R2202 T2832 T2831 arg2Of isolated,were
R2203 T2832 T2833 arg1Of isolated,from
R2204 T2835 T2833 arg2Of regions,from
R2205 T2835 T2834 arg1Of regions,peri-hemorrhage
R2206 T2835 T2836 arg1Of regions,in
R2207 T2839 T2836 arg2Of ganglia,in
R2208 T2839 T2837 arg1Of ganglia,left
R2209 T2839 T2838 arg1Of ganglia,basal
R2210 T2839 T2840 arg1Of ganglia,using
R2211 T2840 T2847 arg1Of using,(
R2212 T2840 T2854 arg1Of using,according
R2213 T2843 T2841 arg1Of Total,Simply
R2214 T2843 T2842 arg1Of Total,P
R2215 T2846 T2840 arg2Of Kit,using
R2216 T2846 T2843 arg1Of Kit,Total
R2217 T2846 T2844 arg1Of Kit,RNA
R2218 T2846 T2845 arg1Of Kit,isolation
R2219 T2848 T2849 arg1Of BioFlux,","
R2220 T2849 T2847 arg2Of ",",(
R2221 T2849 T2851 arg1Of ",",","
R2222 T2850 T2849 arg2Of HZ,","
R2223 T2852 T2851 arg2Of China,","
R2224 T2853 T2847 arg3Of ),(
R2225 T2855 T2854 arg2Of to,according
R2226 T2857 T2856 arg1Of manufacturer,the
R2227 T2857 T2858 arg2Of manufacturer,'s
R2228 T2859 T2855 arg2Of instructions,to
R2229 T2859 T2858 arg1Of instructions,'s
R2230 T2861 T2860 arg1Of quantity,The
R2231 T2861 T2862 arg1Of quantity,of
R2232 T2861 T2865 arg1Of quantity,was
R2233 T2861 T2866 arg2Of quantity,determined
R2234 T2861 T2875 arg1Of quantity,measuring
R2235 T2864 T2862 arg2Of RNA,of
R2236 T2864 T2863 arg1Of RNA,total
R2237 T2866 T2865 arg2Of determined,was
R2238 T2866 T2867 arg1Of determined,on
R2239 T2866 T2874 arg1Of determined,by
R2240 T2869 T2867 arg2Of Nanodrop,on
R2241 T2869 T2868 arg1Of Nanodrop,a
R2242 T2869 T2870 arg1Of Nanodrop,(
R2243 T2872 T2870 arg2Of Spectrophotometer,(
R2244 T2872 T2871 arg1Of Spectrophotometer,ND-1000
R2245 T2873 T2870 arg3Of ),(
R2246 T2875 T2874 arg2Of measuring,by
R2247 T2875 T2878 arg1Of measuring,at
R2248 T2877 T2875 arg2Of density,measuring
R2249 T2877 T2876 arg1Of density,optical
R2250 T2879 T2880 arg1Of A260,and
R2251 T2881 T2880 arg2Of A280 nm,and
R2252 T2882 T2878 arg2Of wavelength,at
R2253 T2882 T2879 arg1Of wavelength,A260
R2254 T2882 T2881 arg1Of wavelength,A280 nm
R2255 T2886 T2885 arg1Of concentrations,the
R2256 T2886 T2887 arg1Of concentrations,of
R2257 T2886 T2889 arg1Of concentrations,were
R2258 T2886 T2890 arg2Of concentrations,adjusted
R2259 T2888 T2887 arg2Of mRNA,of
R2260 T2890 T2883 arg1Of adjusted,Then
R2261 T2890 T2884 arg1Of adjusted,","
R2262 T2890 T2889 arg2Of adjusted,were
R2263 T2890 T2891 arg1Of adjusted,to
R2264 T2892 T2891 arg2Of 1.0 g/mL,to
R2265 T2893 T2894 arg1Of RNA,was
R2266 T2893 T2895 arg2Of RNA,reverse-transcribed
R2267 T2893 T2902 arg1Of RNA,using
R2268 T2895 T2894 arg2Of reverse-transcribed,was
R2269 T2895 T2896 arg1Of reverse-transcribed,into
R2270 T2895 T2898 arg1Of reverse-transcribed,in
R2271 T2895 T2902 modOf reverse-transcribed,using
R2272 T2897 T2896 arg2Of cDNA,into
R2273 T2901 T2898 arg2Of system,in
R2274 T2901 T2899 arg1Of system,20 μL
R2275 T2901 T2900 arg1Of system,reaction
R2276 T2905 T2903 arg1Of Synthesis,Superscript
R2277 T2905 T2904 arg1Of Synthesis,First-Strand
R2278 T2906 T2902 arg2Of Kit,using
R2279 T2906 T2905 arg1Of Kit,Synthesis
R2280 T2906 T2907 arg1Of Kit,for
R2281 T2908 T2907 arg2Of RT-PCR,for
R2282 T2908 T2909 arg1Of RT-PCR,(
R2283 T2908 T2913 arg1Of RT-PCR,under
R2284 T2911 T2909 arg2Of Inc,(
R2285 T2911 T2910 arg1Of Inc,promega
R2286 T2912 T2909 arg3Of ),(
R2287 T2914 T2913 arg2Of conditions,under
R2288 T2914 T2915 arg2Of conditions,described
R2289 T2918 T2915 arg1Of supplier,described
R2290 T2918 T2916 arg2Of supplier,by
R2291 T2918 T2917 arg1Of supplier,the
R2292 T2920 T2919 arg1Of microliter,One
R2293 T2920 T2921 arg1Of microliter,of
R2294 T2920 T2923 arg1Of microliter,was
R2295 T2920 T2924 arg2Of microliter,used
R2296 T2922 T2921 arg2Of cDNA,of
R2297 T2924 T2923 arg2Of used,was
R2298 T2926 T2924 arg3Of amplify,used
R2299 T2926 T2925 arg1Of amplify,to
R2300 T2930 T2926 arg2Of fragment,amplify
R2301 T2930 T2927 arg1Of fragment,the
R2302 T2930 T2928 arg1Of fragment,TLR4
R2303 T2930 T2929 arg1Of fragment,gene
R2304 T2930 T2931 arg1Of fragment,with
R2305 T2934 T2932 arg1Of buffer,1×
R2306 T2934 T2933 arg1Of buffer,PCR
R2307 T2934 T2935 arg1Of buffer,","
R2308 T2935 T2937 arg1Of ",",","
R2309 T2936 T2935 arg2Of 1.5 mM MgCl2,","
R2310 T2937 T2942 arg1Of ",",","
R2311 T2938 T2937 arg2Of 200 μM,","
R2312 T2938 T2939 arg1Of 200 μM,of
R2313 T2941 T2939 arg2Of dNTP,of
R2314 T2941 T2940 arg1Of dNTP,each
R2315 T2942 T2947 arg1Of ",",and
R2316 T2943 T2942 arg2Of 200 μM,","
R2317 T2943 T2944 arg1Of 200 μM,of
R2318 T2945 T2944 arg2Of primers,of
R2319 T2947 T2931 arg2Of and,with
R2320 T2947 T2946 arg1Of and,","
R2321 T2951 T2947 arg2Of polymerase,and
R2322 T2951 T2948 arg1Of polymerase,2u
R2323 T2951 T2949 arg1Of polymerase,Taq
R2324 T2951 T2950 arg1Of polymerase,DNA
R2325 T2955 T2952 arg1Of gene,The
R2326 T2955 T2953 arg1Of gene,house
R2327 T2955 T2954 arg1Of gene,keep
R2328 T2955 T2956 arg1Of gene,(
R2329 T2955 T2959 arg1Of gene,was
R2330 T2955 T2960 arg2Of gene,used
R2331 T2957 T2956 arg2Of β-actin,(
R2332 T2958 T2956 arg3Of ),(
R2333 T2960 T2959 arg2Of used,was
R2334 T2960 T2961 arg1Of used,as
R2335 T2964 T2961 arg2Of control,as
R2336 T2964 T2962 arg1Of control,an
R2337 T2964 T2963 arg1Of control,internal
R2338 T2968 T2965 arg1Of sequences,The
R2339 T2968 T2966 arg1Of sequences,PCR
R2340 T2968 T2967 arg1Of sequences,primer
R2341 T2968 T2969 arg1Of sequences,were
R2342 T2968 T2970 arg2Of sequences,designed
R2343 T2970 T2969 arg2Of designed,were
R2344 T2970 T2971 arg1Of designed,according
R2345 T2972 T2971 arg2Of to,according
R2346 T2974 T2975 arg1Of TLR4,and
R2347 T2975 T2972 arg2Of and,to
R2348 T2975 T2973 arg1Of and,the
R2349 T2975 T2979 arg2Of and,reported
R2350 T2978 T2975 arg2Of sequences,and
R2351 T2978 T2976 arg1Of sequences,β-actin
R2352 T2978 T2977 arg1Of sequences,gene
R2353 T2979 T2980 arg1Of reported,in
R2354 T2981 T2980 arg2Of GenBank,in
R2355 T2982 T2983 arg1Of TLR4,(
R2356 T2982 T2986 arg1Of TLR4,:
R2357 T2982 T2987 arg1Of TLR4,forward
R2358 T2982 T2988 arg1Of TLR4,:
R2359 T2984 T2983 arg2Of 145 bp,(
R2360 T2985 T2983 arg3Of ),(
R2361 T2989 T2990 arg1Of 5′-AGCTTTGGTCAGTTGGCTCT-3′,;
R2362 T2990 T2992 arg1Of ;,:
R2363 T2991 T2990 arg2Of reverse,;
R2364 T2993 T2994 arg1Of 5′-CAGGATGACACCATTGAAGC-3′,;
R2365 T2994 T2996 arg1Of ;,(
R2366 T2994 T2999 arg1Of ;,:
R2367 T2994 T3000 arg1Of ;,forward
R2368 T2994 T3001 arg1Of ;,:
R2369 T2995 T2994 arg2Of β-actin,;
R2370 T2997 T2996 arg2Of 701 bp,(
R2371 T2998 T2996 arg3Of ),(
R2372 T3002 T3003 arg1Of 5′-GCCAACCGTGAAAAAGATG-3′,;
R2373 T3003 T3005 arg1Of ;,:
R2374 T3004 T3003 arg2Of reverse,;
R2375 T3008 T3007 arg1Of cycles,Reaction
R2376 T3008 T3009 arg1Of cycles,were
R2377 T3008 T3010 arg2Of cycles,performed
R2378 T3010 T3009 arg2Of performed,were
R2379 T3010 T3011 arg1Of performed,at
R2380 T3010 T3015 arg1Of performed,:
R2381 T3014 T3011 arg2Of condition,at
R2382 T3014 T3012 arg1Of condition,the
R2383 T3014 T3013 arg1Of condition,following
R2384 T3017 T3016 arg1Of 2 min,94°C
R2385 T3017 T3018 arg1Of 2 min,","
R2386 T3018 T3021 arg1Of ",",","
R2387 T3020 T3018 arg2Of 40 s,","
R2388 T3020 T3019 arg1Of 40 s,94°C
R2389 T3021 T3025 arg1Of ",",and
R2390 T3023 T3021 arg2Of 40 s,","
R2391 T3023 T3022 arg1Of 40 s,55°C
R2392 T3025 T3024 arg1Of and,","
R2393 T3027 T3025 arg2Of 60 s,and
R2394 T3027 T3026 arg1Of 60 s,72°C
R2395 T3029 T3028 arg1Of reaction,The
R2396 T3029 T3030 arg1Of reaction,was
R2397 T3029 T3031 arg2Of reaction,stopped
R2398 T3031 T3030 arg2Of stopped,was
R2399 T3031 T3032 arg1Of stopped,after
R2400 T3034 T3032 arg2Of cycles,after
R2401 T3034 T3033 arg1Of cycles,31
R2402 T3036 T3035 arg1Of products,PCR
R2403 T3036 T3037 arg1Of products,were
R2404 T3036 T3038 arg2Of products,electrophoresed
R2405 T3038 T3037 arg2Of electrophoresed,were
R2406 T3038 T3039 arg1Of electrophoresed,through
R2407 T3041 T3039 arg2Of gel,through
R2408 T3041 T3040 arg1Of gel,agarose
R2409 T3043 T3042 arg1Of Acquisition,Image
R2410 T3043 T3044 arg1Of Acquisition,and
R2411 T3044 T3047 arg1Of and,were
R2412 T3044 T3048 arg2Of and,used
R2413 T3046 T3044 arg2Of software,and
R2414 T3046 T3045 arg1Of software,Analysis
R2415 T3048 T3047 arg2Of used,were
R2416 T3048 T3049 modOf used,to
R2417 T3050 T3049 arg1Of determine,to
R2418 T3052 T3050 arg2Of densities,determine
R2419 T3052 T3051 arg1Of densities,band
R2420 T3054 T3053 arg1Of abundance,The
R2421 T3054 T3055 arg1Of abundance,of
R2422 T3054 T3058 arg1Of abundance,was
R2423 T3054 T3059 arg2Of abundance,expressed
R2424 T3057 T3055 arg2Of mRNA,of
R2425 T3057 T3056 arg1Of mRNA,TLR4
R2426 T3059 T3058 arg2Of expressed,was
R2427 T3059 T3060 arg1Of expressed,as
R2428 T3062 T3060 arg2Of ratio,as
R2429 T3062 T3061 arg1Of ratio,the
R2430 T3062 T3063 arg1Of ratio,of
R2431 T3062 T3066 arg1Of ratio,to
R2432 T3065 T3063 arg2Of mRNA,of
R2433 T3065 T3064 arg1Of mRNA,TLR4
R2434 T3068 T3066 arg2Of mRNA,to
R2435 T3068 T3067 arg1Of mRNA,β-actin

bionlp-st-ge-2016-test-proteins

Id Subject Object Predicate Lexical cue
T2770 1450-1454 Protein denotes TLR4
T2769 1059-1066 Protein denotes β-actin
T2768 970-974 Protein denotes TLR4
T2767 926-933 Protein denotes β-actin
T2766 917-921 Protein denotes TLR4
T2765 819-826 Protein denotes β-actin
T2764 798-817 Protein denotes The house keep gene
T2763 778-796 Protein denotes Taq DNA polymerase
T2762 678-682 Protein denotes TLR4
T2761 18-22 Protein denotes TLR4
T2772 1503-1510 Protein denotes β-actin
T2771 1490-1494 Protein denotes TLR4

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T3383 1503-1510 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3382 1059-1066 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3381 926-933 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3380 819-826 http://www.uniprot.org/uniprot/P60709 denotes β-actin
T3379 1490-1494 http://www.uniprot.org/uniprot/O00206 denotes TLR4
T3378 1450-1454 http://www.uniprot.org/uniprot/O00206 denotes TLR4
T3377 970-974 http://www.uniprot.org/uniprot/O00206 denotes TLR4
T3376 917-921 http://www.uniprot.org/uniprot/O00206 denotes TLR4
T3375 678-682 http://www.uniprot.org/uniprot/O00206 denotes TLR4
T3374 18-22 http://www.uniprot.org/uniprot/O00206 denotes TLR4

GO-BP

Id Subject Object Predicate Lexical cue
T2775 548-557 http://purl.obolibrary.org/obo/GO_0009058 denotes Synthesis
T2774 566-568 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T2773 45-47 http://purl.obolibrary.org/obo/GO_0003964 denotes RT

GO-MF

Id Subject Object Predicate Lexical cue
T2777 566-568 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T2776 45-47 http://purl.obolibrary.org/obo/GO_0003964 denotes RT

sentences

Id Subject Object Predicate Lexical cue
T2748 1433-1516 Sentence denotes The abundance of TLR4 mRNA was expressed as the ratio of TLR4 mRNA to β-actin mRNA.
T2747 1354-1432 Sentence denotes Image Acquisition and Analysis software were used to determine band densities.
T2746 1299-1353 Sentence denotes PCR products were electrophoresed through agarose gel.
T2745 1257-1298 Sentence denotes The reaction was stopped after 31 cycles.
T2744 1208-1256 Sentence denotes 94°C 2 min, 94°C 40 s, 55°C 40 s, and 72°C 60 s.
T2743 1149-1207 Sentence denotes Reaction cycles were performed at the following condition:
T2742 1122-1148 Sentence denotes 5′-CCAGGATAGAGCCACCAAT-3′.
T2741 1086-1121 Sentence denotes 5′-GCCAACCGTGAAAAAGATG-3′; reverse:
T2740 1031-1085 Sentence denotes 5′-CAGGATGACACCATTGAAGC-3′; β-actin (701 bp): forward:
T2739 994-1030 Sentence denotes 5′-AGCTTTGGTCAGTTGGCTCT-3′; reverse:
T2738 970-993 Sentence denotes TLR4 (145 bp): forward:
T2737 861-969 Sentence denotes The PCR primer sequences were designed according to the TLR4 and β-actin gene sequences reported in GenBank.
T2736 798-860 Sentence denotes The house keep gene (β-actin) was used as an internal control.
T2735 631-797 Sentence denotes One microliter of cDNA was used to amplify the TLR4 gene fragment with 1× PCR buffer, 1.5 mM MgCl2, 200 μM of each dNTP, 200 μM of primers, and 2u Taq DNA polymerase.
T2734 454-630 Sentence denotes RNA was reverse-transcribed into cDNA in 20 μL reaction system using Superscript First-Strand Synthesis Kit for RT-PCR (promega Inc) under conditions described by the supplier.
T2733 394-453 Sentence denotes Then, the concentrations of mRNA were adjusted to 1.0 g/mL.
T2732 250-393 Sentence denotes The quantity of total RNA was determined on a Nanodrop (ND-1000 Spectrophotometer) by measuring optical density at A260 and A280 nm wavelength.
T2731 52-249 Sentence denotes Briefly, total RNA samples were isolated from peri-hemorrhage regions in left basal ganglia using Simply P Total RNA isolation Kit (BioFlux, HZ, China) according to the manufacturer's instructions.
T2730 5-51 Sentence denotes Detection of TLR4 mRNA by the Method of RT-PCR
T2729 0-4 Sentence denotes 2.3.
T51 0-4 Sentence denotes 2.3.
T52 5-51 Sentence denotes Detection of TLR4 mRNA by the Method of RT-PCR
T53 52-249 Sentence denotes Briefly, total RNA samples were isolated from peri-hemorrhage regions in left basal ganglia using Simply P Total RNA isolation Kit (BioFlux, HZ, China) according to the manufacturer's instructions.
T54 250-393 Sentence denotes The quantity of total RNA was determined on a Nanodrop (ND-1000 Spectrophotometer) by measuring optical density at A260 and A280 nm wavelength.
T55 394-453 Sentence denotes Then, the concentrations of mRNA were adjusted to 1.0 g/mL.
T56 454-630 Sentence denotes RNA was reverse-transcribed into cDNA in 20 μL reaction system using Superscript First-Strand Synthesis Kit for RT-PCR (promega Inc) under conditions described by the supplier.
T57 631-797 Sentence denotes One microliter of cDNA was used to amplify the TLR4 gene fragment with 1× PCR buffer, 1.5 mM MgCl2, 200 μM of each dNTP, 200 μM of primers, and 2u Taq DNA polymerase.
T58 798-860 Sentence denotes The house keep gene (β-actin) was used as an internal control.
T59 861-969 Sentence denotes The PCR primer sequences were designed according to the TLR4 and β-actin gene sequences reported in GenBank.
T60 970-993 Sentence denotes TLR4 (145 bp): forward:
T61 994-1030 Sentence denotes 5′-AGCTTTGGTCAGTTGGCTCT-3′; reverse:
T62 1031-1085 Sentence denotes 5′-CAGGATGACACCATTGAAGC-3′; β-actin (701 bp): forward:
T63 1086-1121 Sentence denotes 5′-GCCAACCGTGAAAAAGATG-3′; reverse:
T64 1122-1148 Sentence denotes 5′-CCAGGATAGAGCCACCAAT-3′.
T65 1149-1207 Sentence denotes Reaction cycles were performed at the following condition:
T66 1208-1256 Sentence denotes 94°C 2 min, 94°C 40 s, 55°C 40 s, and 72°C 60 s.
T67 1257-1298 Sentence denotes The reaction was stopped after 31 cycles.
T68 1299-1353 Sentence denotes PCR products were electrophoresed through agarose gel.
T69 1354-1432 Sentence denotes Image Acquisition and Analysis software were used to determine band densities.
T70 1433-1516 Sentence denotes The abundance of TLR4 mRNA was expressed as the ratio of TLR4 mRNA to β-actin mRNA.

simple1

Id Subject Object Predicate Lexical cue
T2815 1503-1510 Protein denotes β-actin
T2814 1490-1494 Protein denotes TLR4
T2813 1450-1454 Protein denotes TLR4
T2812 1059-1066 Protein denotes β-actin
T2811 970-974 Protein denotes TLR4
T2810 926-933 Protein denotes β-actin
T2809 917-921 Protein denotes TLR4
T2808 819-826 Protein denotes β-actin
T2807 798-817 Protein denotes The house keep gene
T2806 778-796 Protein denotes Taq DNA polymerase
T2805 678-682 Protein denotes TLR4
T2804 18-22 Protein denotes TLR4

BioNLP16_DUT

Id Subject Object Predicate Lexical cue
T3473 1437-1446 Transcription denotes abundance
T3472 1503-1510 Protein denotes β-actin
T3471 1490-1494 Protein denotes TLR4
T3470 1450-1454 Protein denotes TLR4
T3469 1059-1066 Protein denotes β-actin
T3468 970-974 Protein denotes TLR4
T3467 926-933 Protein denotes β-actin
T3466 917-921 Protein denotes TLR4
T3465 819-826 Protein denotes β-actin
T3464 798-817 Protein denotes The house keep gene
T3463 778-796 Protein denotes Taq DNA polymerase
T3462 678-682 Protein denotes TLR4
T3461 18-22 Protein denotes TLR4
R2753 T3470 T3473 themeOf TLR4,abundance

BioNLP16_Messiy

Id Subject Object Predicate Lexical cue
T3396 1464-1473 Transcription denotes expressed
T3395 1503-1510 Protein denotes β-actin
T3394 1490-1494 Protein denotes TLR4
T3393 1450-1454 Protein denotes TLR4
T3392 1059-1066 Protein denotes β-actin
T3391 970-974 Protein denotes TLR4
T3390 926-933 Protein denotes β-actin
T3389 917-921 Protein denotes TLR4
T3388 819-826 Protein denotes β-actin
T3387 798-817 Protein denotes The house keep gene
T3386 778-796 Protein denotes Taq DNA polymerase
T3385 678-682 Protein denotes TLR4
T3384 18-22 Protein denotes TLR4
R2741 T3393 T3396 themeOf TLR4,expressed

DLUT931

Id Subject Object Predicate Lexical cue
T3413 1495-1499 Transcription denotes mRNA
T3412 1455-1459 Transcription denotes mRNA
T3411 1437-1446 Transcription denotes abundance
T3410 23-27 Transcription denotes mRNA
T3409 5-14 Transcription denotes Detection
T3408 1503-1510 Protein denotes β-actin
T3407 1490-1494 Protein denotes TLR4
T3406 1450-1454 Protein denotes TLR4
T3405 1059-1066 Protein denotes β-actin
T3404 970-974 Protein denotes TLR4
T3403 926-933 Protein denotes β-actin
T3402 917-921 Protein denotes TLR4
T3401 819-826 Protein denotes β-actin
T3400 798-817 Protein denotes The house keep gene
T3399 778-796 Protein denotes Taq DNA polymerase
T3398 678-682 Protein denotes TLR4
T3397 18-22 Protein denotes TLR4
T3414 1511-1515 Transcription denotes mRNA
R2745 T3406 T3412 themeOf TLR4,mRNA
R2746 T3407 T3413 themeOf TLR4,mRNA
R2747 T3408 T3414 themeOf β-actin,mRNA
R2742 T3397 T3409 themeOf TLR4,Detection
R2743 T3397 T3410 themeOf TLR4,mRNA
R2744 T3406 T3411 themeOf TLR4,abundance

bionlp-st-ge-2016-test-ihmc

Id Subject Object Predicate Lexical cue
T3491 1299-1302 Protein denotes PCR
T3490 819-826 Protein denotes β-actin
T3489 961-964 Protein denotes Gen
T3488 798-827 Protein denotes The house keep gene (β-actin)
T3487 1031-1066 Protein denotes 5′-CAGGATGACACCATTGAAGC-3′; β-actin
T3486 15-27 Protein denotes of TLR4 mRNA
T3485 970-974 Protein denotes TLR4
T3484 674-716 Protein denotes the TLR4 gene fragment with 1× PCR buffer,
T3483 67-70 Protein denotes RNA
T3482 865-868 Protein denotes PCR
T3481 705-708 Protein denotes PCR
T3480 678-682 Protein denotes TLR4
T3479 18-22 Protein denotes TLR4
T3478 365-369 Entity denotes A260
T3477 419-426 Protein denotes of mRNA
T3476 523-561 Protein denotes Superscript First-Strand Synthesis Kit
T3475 165-168 Protein denotes RNA
T3474 775-796 Entity denotes 2u Taq DNA polymerase
T3460 1490-1494 Protein denotes TLR4
T3459 913-921 Protein denotes the TLR4
T3458 1487-1499 Protein denotes of TLR4 mRNA
T3457 1447-1459 Protein denotes of TLR4 mRNA
T3456 374-379 Entity denotes A280 
T3455 1500-1515 Protein denotes to β-actin mRNA
T3454 1450-1454 Protein denotes TLR4
T3453 775-796 Protein denotes 2u Taq DNA polymerase
T3452 1341-1352 Entity denotes agarose gel
T3451 674-716 Protein denotes the TLR4 gene fragment with 1× PCR buffer,
T3450 926-968 Protein denotes β-actin gene sequences reported in GenBank
T3449 747-751 Entity denotes NTP,
T3448 1341-1348 Entity denotes agarose
T3447 566-586 Protein denotes RT-PCR (promega Inc)
T3446 1500-1515 Protein denotes to β-actin mRNA
T3445 454-457 Protein denotes RNA
T3444 157-182 Protein denotes P Total RNA isolation Kit
T3443 263-275 Protein denotes of total RNA
T3442 961-968 Protein denotes GenBank
T3441 42-51 Protein denotes of RT-PCR

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T3088 98-113 JJ denotes peri-hemorrhage
T3373 1515-1516 . denotes .
T3372 1511-1515 NNP denotes mRNA
T3371 1503-1510 VB denotes β-actin
T3370 1500-1502 TO denotes to
T3369 1495-1499 NNP denotes mRNA
T3368 1490-1494 CD denotes TLR4
T3367 1487-1489 IN denotes of
T3366 1481-1486 NN denotes ratio
T3365 1477-1480 DT denotes the
T3364 1474-1476 IN denotes as
T3363 1464-1473 VBN denotes expressed
T3362 1460-1463 VBD denotes was
T3361 1455-1459 NNP denotes mRNA
T3360 1450-1454 CD denotes TLR4
T3359 1447-1449 IN denotes of
T3358 1437-1446 NN denotes abundance
T3357 1433-1436 DT denotes The
T3356 1431-1432 . denotes .
T3355 1422-1431 NNS denotes densities
T3354 1417-1421 NN denotes band
T3353 1407-1416 VB denotes determine
T3352 1404-1406 TO denotes to
T3351 1399-1403 VBN denotes used
T3350 1394-1398 VBD denotes were
T3349 1385-1393 NN denotes software
T3348 1376-1384 NNP denotes Analysis
T3347 1372-1375 CC denotes and
T3346 1360-1371 NNP denotes Acquisition
T3345 1354-1359 NN denotes Image
T3344 1352-1353 . denotes .
T3343 1349-1352 NN denotes gel
T3342 1341-1348 JJ denotes agarose
T3341 1333-1340 IN denotes through
T3340 1317-1332 VBN denotes electrophoresed
T3339 1312-1316 VBD denotes were
T3338 1303-1311 NNS denotes products
T3337 1299-1302 NNP denotes PCR
T3336 1297-1298 . denotes .
T3335 1291-1297 NNS denotes cycles
T3334 1288-1290 CD denotes 31
T3333 1282-1287 RB denotes after
T3332 1274-1281 VBN denotes stopped
T3331 1270-1273 VBD denotes was
T3330 1261-1269 NN denotes reaction
T3329 1257-1260 DT denotes The
T3328 1255-1256 . denotes .
T3327 1254-1255 PRP denotes s
T3326 1251-1253 CD denotes 60
T3325 1249-1250 NNP denotes C
T3324 1248-1249 NN denotes °
T3323 1246-1248 CD denotes 72
T3322 1242-1245 CC denotes and
T3321 1240-1241 , denotes ,
T3320 1239-1240 PRP denotes s
T3319 1236-1238 CD denotes 40
T3318 1234-1235 NNP denotes C
T3317 1233-1234 NN denotes °
T3316 1231-1233 CD denotes 55
T3315 1229-1230 , denotes ,
T3314 1228-1229 PRP denotes s
T3313 1225-1227 CD denotes 40
T3312 1223-1224 NNP denotes C
T3311 1222-1223 NN denotes °
T3310 1220-1222 CD denotes 94
T3309 1218-1219 , denotes ,
T3308 1215-1218 NN denotes min
T3307 1213-1214 CD denotes 2
T3306 1211-1212 NNP denotes C
T3305 1210-1211 NN denotes °
T3304 1208-1210 CD denotes 94
T3303 1206-1207 : denotes :
T3302 1197-1206 NN denotes condition
T3301 1187-1196 JJ denotes following
T3300 1183-1186 DT denotes the
T3299 1180-1182 IN denotes at
T3298 1170-1179 VBN denotes performed
T3297 1165-1169 VBD denotes were
T3296 1158-1164 NNS denotes cycles
T3295 1149-1157 NN denotes Reaction
T3294 1147-1148 . denotes .
T3293 1146-1147 NN denotes ′
T3292 1125-1146 NN denotes CCAGGATAGAGCCACCAAT-3
T3291 1124-1125 : denotes -
T3290 1123-1124 SYM denotes ′
T3289 1122-1123 CD denotes 5
T3288 1120-1121 : denotes :
T3287 1113-1120 NN denotes reverse
T3286 1111-1112 : denotes ;
T3285 1110-1111 NN denotes ′
T3284 1089-1110 NN denotes GCCAACCGTGAAAAAGATG-3
T3283 1088-1089 : denotes -
T3282 1087-1088 SYM denotes ′
T3281 1086-1087 CD denotes 5
T3280 1084-1085 : denotes :
T3279 1077-1084 RB denotes forward
T3278 1075-1076 : denotes :
T3277 1074-1075 -RRB- denotes )
T3276 1072-1074 NN denotes bp
T3275 1068-1071 CD denotes 701
T3274 1067-1068 -LRB- denotes (
T3273 1059-1066 NN denotes β-actin
T3272 1057-1058 : denotes ;
T3271 1056-1057 NN denotes ′
T3270 1034-1056 NN denotes CAGGATGACACCATTGAAGC-3
T3269 1033-1034 : denotes -
T3268 1032-1033 SYM denotes ′
T3267 1031-1032 CD denotes 5
T3266 1029-1030 : denotes :
T3265 1022-1029 NN denotes reverse
T3264 1020-1021 : denotes ;
T3263 1019-1020 NN denotes ′
T3262 997-1019 NN denotes AGCTTTGGTCAGTTGGCTCT-3
T3261 996-997 : denotes -
T3260 995-996 SYM denotes ′
T3259 994-995 CD denotes 5
T3258 992-993 : denotes :
T3257 985-992 RB denotes forward
T3256 983-984 : denotes :
T3255 982-983 -RRB- denotes )
T3254 980-982 NN denotes bp
T3253 976-979 CD denotes 145
T3252 975-976 -LRB- denotes (
T3251 970-974 CD denotes TLR4
T3250 968-969 . denotes .
T3249 961-968 NNP denotes GenBank
T3248 958-960 IN denotes in
T3247 949-957 VBD denotes reported
T3246 939-948 NNS denotes sequences
T3245 934-938 NN denotes gene
T3244 926-933 JJ denotes β-actin
T3243 922-925 CC denotes and
T3242 917-921 CD denotes TLR4
T3241 913-916 DT denotes the
T3240 910-912 TO denotes to
T3239 900-909 VBG denotes according
T3238 891-899 VBN denotes designed
T3237 886-890 VBD denotes were
T3236 876-885 NNS denotes sequences
T3235 869-875 NN denotes primer
T3234 865-868 NNP denotes PCR
T3233 861-864 DT denotes The
T3232 859-860 . denotes .
T3231 852-859 NN denotes control
T3230 843-851 JJ denotes internal
T3229 840-842 DT denotes an
T3228 837-839 IN denotes as
T3227 832-836 VBN denotes used
T3226 828-831 VBD denotes was
T3225 826-827 -RRB- denotes )
T3224 819-826 NN denotes β-actin
T3223 818-819 -LRB- denotes (
T3222 813-817 NN denotes gene
T3221 808-812 VB denotes keep
T3220 802-807 NN denotes house
T3219 798-801 DT denotes The
T3218 796-797 . denotes .
T3217 786-796 NN denotes polymerase
T3216 782-785 NNP denotes DNA
T3215 778-781 NNP denotes Taq
T3214 775-777 CD denotes 2u
T3213 771-774 CC denotes and
T3212 769-770 , denotes ,
T3211 762-769 NNS denotes primers
T3210 759-761 IN denotes of
T3209 756-758 NN denotes μM
T3208 752-755 CD denotes 200
T3207 750-751 , denotes ,
T3206 746-750 NNP denotes dNTP
T3205 741-745 DT denotes each
T3204 738-740 IN denotes of
T3203 735-737 NN denotes μM
T3202 731-734 CD denotes 200
T3201 729-730 , denotes ,
T3200 724-729 NNP denotes MgCl2
T3199 721-723 NNP denotes mM
T3198 717-720 CD denotes 1.5
T3087 93-97 IN denotes from
T3086 84-92 VBN denotes isolated
T3085 79-83 VBD denotes were
T3084 71-78 NNS denotes samples
T3083 67-70 NNP denotes RNA
T3082 61-66 JJ denotes total
T3081 59-60 , denotes ,
T3080 52-59 NNP denotes Briefly
T3079 45-51 NNP denotes RT-PCR
T3078 42-44 IN denotes of
T3077 35-41 NNP denotes Method
T3076 31-34 DT denotes the
T3075 28-30 IN denotes by
T3074 23-27 NNP denotes mRNA
T3073 18-22 NNP denotes TLR4
T3072 15-17 IN denotes of
T3071 5-14 NNP denotes Detection
T3070 3-4 . denotes .
T3069 0-3 CD denotes 2.3
T3197 715-716 , denotes ,
T3196 709-715 NN denotes buffer
T3195 705-708 NNP denotes PCR
T3194 703-704 CD denotes ×
T3193 702-703 CD denotes 1
T3192 697-701 IN denotes with
T3191 688-696 NN denotes fragment
T3190 683-687 NN denotes gene
T3189 678-682 CD denotes TLR4
T3188 674-677 DT denotes the
T3187 666-673 VB denotes amplify
T3186 663-665 TO denotes to
T3185 658-662 VBN denotes used
T3184 654-657 VBD denotes was
T3183 649-653 NNP denotes cDNA
T3182 646-648 IN denotes of
T3181 635-645 NN denotes microliter
T3180 631-634 CD denotes One
T3179 629-630 . denotes .
T3178 621-629 NN denotes supplier
T3177 617-620 DT denotes the
T3176 614-616 IN denotes by
T3175 604-613 VBN denotes described
T3174 593-603 NNS denotes conditions
T3173 587-592 IN denotes under
T3172 585-586 -RRB- denotes )
T3171 582-585 NNP denotes Inc
T3170 574-581 JJ denotes promega
T3169 573-574 -LRB- denotes (
T3168 566-572 NNP denotes RT-PCR
T3167 562-565 IN denotes for
T3166 558-561 NNP denotes Kit
T3165 548-557 NNP denotes Synthesis
T3164 535-547 NNP denotes First-Strand
T3163 523-534 NNP denotes Superscript
T3162 517-522 VBG denotes using
T3161 510-516 NN denotes system
T3160 501-509 NN denotes reaction
T3159 498-500 NN denotes μL
T3158 495-497 CD denotes 20
T3157 492-494 IN denotes in
T3156 487-491 NNP denotes cDNA
T3155 482-486 IN denotes into
T3154 462-481 VBN denotes reverse-transcribed
T3153 458-461 VBD denotes was
T3152 454-457 NNP denotes RNA
T3151 452-453 . denotes .
T3150 448-452 NNP denotes g/mL
T3149 444-447 CD denotes 1.0
T3148 441-443 TO denotes to
T3147 432-440 VBN denotes adjusted
T3146 427-431 VBD denotes were
T3145 422-426 NNP denotes mRNA
T3144 419-421 IN denotes of
T3143 404-418 NNS denotes concentrations
T3142 400-403 DT denotes the
T3141 398-399 , denotes ,
T3140 394-398 RB denotes Then
T3139 392-393 . denotes .
T3138 382-392 NN denotes wavelength
T3137 379-381 NN denotes nm
T3136 374-378 CD denotes A280
T3135 370-373 CC denotes and
T3134 365-369 CD denotes A260
T3133 362-364 IN denotes at
T3132 354-361 NN denotes density
T3131 346-353 JJ denotes optical
T3130 336-345 VBG denotes measuring
T3129 333-335 IN denotes by
T3128 331-332 -RRB- denotes )
T3127 314-331 NNP denotes Spectrophotometer
T3126 306-313 NNP denotes ND-1000
T3125 305-306 -LRB- denotes (
T3124 296-304 NNP denotes Nanodrop
T3123 294-295 DT denotes a
T3122 291-293 IN denotes on
T3121 280-290 VBN denotes determined
T3120 276-279 VBD denotes was
T3119 272-275 NNP denotes RNA
T3118 266-271 JJ denotes total
T3117 263-265 IN denotes of
T3116 254-262 NN denotes quantity
T3115 250-253 DT denotes The
T3114 248-249 . denotes .
T3113 236-248 NNS denotes instructions
T3112 233-235 POS denotes 's
T3111 221-233 NN denotes manufacturer
T3110 217-220 DT denotes the
T3109 214-216 TO denotes to
T3108 204-213 VBG denotes according
T3107 202-203 -RRB- denotes )
T3106 197-202 NNP denotes China
T3105 195-196 , denotes ,
T3104 193-195 NNP denotes HZ
T3103 191-192 , denotes ,
T3102 184-191 NNP denotes BioFlux
T3101 183-184 -LRB- denotes (
T3100 179-182 NN denotes Kit
T3099 169-178 NN denotes isolation
T3098 165-168 NNP denotes RNA
T3097 159-164 NNP denotes Total
T3096 157-158 NNP denotes P
T3095 150-156 RB denotes Simply
T3094 144-149 VBG denotes using
T3093 136-143 NN denotes ganglia
T3092 130-135 JJ denotes basal
T3091 125-129 JJ denotes left
T3090 122-124 IN denotes in
T3089 114-121 NNS denotes regions
R2436 T3069 T3069 ROOT 2.3,2.3
R2437 T3070 T3069 punct .,2.3
R2438 T3071 T3086 advcl Detection,isolated
R2439 T3072 T3071 prep of,Detection
R2440 T3073 T3074 compound TLR4,mRNA
R2441 T3074 T3072 pobj mRNA,of
R2442 T3075 T3071 prep by,Detection
R2443 T3076 T3077 det the,Method
R2444 T3077 T3075 pobj Method,by
R2445 T3078 T3077 prep of,Method
R2446 T3079 T3080 compound RT-PCR,Briefly
R2447 T3080 T3078 pobj Briefly,of
R2448 T3081 T3086 punct ",",isolated
R2449 T3082 T3084 amod total,samples
R2450 T3083 T3084 compound RNA,samples
R2451 T3084 T3086 nsubjpass samples,isolated
R2452 T3085 T3086 auxpass were,isolated
R2453 T3086 T3086 ROOT isolated,isolated
R2454 T3087 T3086 prep from,isolated
R2455 T3088 T3089 amod peri-hemorrhage,regions
R2456 T3089 T3087 pobj regions,from
R2457 T3090 T3087 prep in,from
R2458 T3091 T3093 amod left,ganglia
R2459 T3092 T3093 amod basal,ganglia
R2460 T3093 T3090 pobj ganglia,in
R2461 T3094 T3086 advcl using,isolated
R2462 T3095 T3100 advmod Simply,Kit
R2463 T3096 T3100 nmod P,Kit
R2464 T3097 T3100 compound Total,Kit
R2465 T3098 T3099 compound RNA,isolation
R2466 T3099 T3100 compound isolation,Kit
R2467 T3100 T3094 dobj Kit,using
R2468 T3101 T3102 punct (,BioFlux
R2469 T3102 T3100 appos BioFlux,Kit
R2470 T3103 T3102 punct ",",BioFlux
R2471 T3104 T3102 conj HZ,BioFlux
R2472 T3105 T3104 punct ",",HZ
R2473 T3106 T3104 npadvmod China,HZ
R2474 T3107 T3104 punct ),HZ
R2475 T3108 T3086 prep according,isolated
R2476 T3109 T3108 prep to,according
R2477 T3110 T3111 det the,manufacturer
R2478 T3111 T3113 poss manufacturer,instructions
R2479 T3112 T3111 case 's,manufacturer
R2480 T3113 T3109 pobj instructions,to
R2481 T3114 T3086 punct .,isolated
R2482 T3115 T3116 det The,quantity
R2483 T3116 T3121 nsubjpass quantity,determined
R2484 T3117 T3116 prep of,quantity
R2485 T3118 T3119 amod total,RNA
R2486 T3119 T3117 pobj RNA,of
R2487 T3120 T3121 auxpass was,determined
R2488 T3121 T3121 ROOT determined,determined
R2489 T3122 T3121 prep on,determined
R2490 T3123 T3124 det a,Nanodrop
R2491 T3124 T3122 pobj Nanodrop,on
R2492 T3125 T3124 punct (,Nanodrop
R2493 T3126 T3127 compound ND-1000,Spectrophotometer
R2494 T3127 T3124 appos Spectrophotometer,Nanodrop
R2495 T3128 T3124 punct ),Nanodrop
R2496 T3129 T3121 prep by,determined
R2497 T3130 T3129 pcomp measuring,by
R2498 T3131 T3132 amod optical,density
R2499 T3132 T3130 dobj density,measuring
R2500 T3133 T3130 prep at,measuring
R2501 T3134 T3133 pobj A260,at
R2502 T3135 T3134 cc and,A260
R2503 T3136 T3134 conj A280,A260
R2504 T3137 T3138 compound nm,wavelength
R2505 T3138 T3130 npadvmod wavelength,measuring
R2506 T3139 T3121 punct .,determined
R2507 T3140 T3147 advmod Then,adjusted
R2508 T3141 T3147 punct ",",adjusted
R2509 T3142 T3143 det the,concentrations
R2510 T3143 T3147 nsubjpass concentrations,adjusted
R2511 T3144 T3143 prep of,concentrations
R2512 T3145 T3144 pobj mRNA,of
R2513 T3146 T3147 auxpass were,adjusted
R2514 T3147 T3147 ROOT adjusted,adjusted
R2515 T3148 T3147 prep to,adjusted
R2516 T3149 T3150 nummod 1.0,g/mL
R2517 T3150 T3148 pobj g/mL,to
R2518 T3151 T3147 punct .,adjusted
R2519 T3152 T3154 nsubjpass RNA,reverse-transcribed
R2520 T3153 T3154 auxpass was,reverse-transcribed
R2521 T3154 T3154 ROOT reverse-transcribed,reverse-transcribed
R2522 T3155 T3154 prep into,reverse-transcribed
R2523 T3156 T3155 pobj cDNA,into
R2524 T3157 T3154 prep in,reverse-transcribed
R2525 T3158 T3161 nummod 20,system
R2526 T3159 T3161 compound μL,system
R2527 T3160 T3161 compound reaction,system
R2528 T3161 T3157 pobj system,in
R2529 T3162 T3154 advcl using,reverse-transcribed
R2530 T3163 T3166 compound Superscript,Kit
R2531 T3164 T3166 compound First-Strand,Kit
R2532 T3165 T3166 compound Synthesis,Kit
R2533 T3166 T3162 dobj Kit,using
R2534 T3167 T3162 prep for,using
R2535 T3168 T3167 pobj RT-PCR,for
R2536 T3169 T3171 punct (,Inc
R2537 T3170 T3171 compound promega,Inc
R2538 T3171 T3168 appos Inc,RT-PCR
R2539 T3172 T3168 punct ),RT-PCR
R2540 T3173 T3162 prep under,using
R2541 T3174 T3173 pobj conditions,under
R2542 T3175 T3174 acl described,conditions
R2543 T3176 T3175 agent by,described
R2544 T3177 T3178 det the,supplier
R2545 T3178 T3176 pobj supplier,by
R2546 T3179 T3154 punct .,reverse-transcribed
R2547 T3180 T3181 nummod One,microliter
R2548 T3181 T3185 nsubjpass microliter,used
R2549 T3182 T3181 prep of,microliter
R2550 T3183 T3182 pobj cDNA,of
R2551 T3184 T3185 auxpass was,used
R2552 T3185 T3185 ROOT used,used
R2553 T3186 T3187 aux to,amplify
R2554 T3187 T3185 xcomp amplify,used
R2555 T3188 T3191 det the,fragment
R2556 T3189 T3191 nummod TLR4,fragment
R2557 T3190 T3191 compound gene,fragment
R2558 T3191 T3187 dobj fragment,amplify
R2559 T3192 T3187 prep with,amplify
R2560 T3193 T3196 nummod 1,buffer
R2561 T3194 T3196 nummod ×,buffer
R2562 T3195 T3196 compound PCR,buffer
R2563 T3196 T3192 pobj buffer,with
R2564 T3197 T3196 punct ",",buffer
R2565 T3198 T3200 nummod 1.5,MgCl2
R2566 T3199 T3200 compound mM,MgCl2
R2567 T3200 T3196 appos MgCl2,buffer
R2568 T3201 T3200 punct ",",MgCl2
R2569 T3202 T3203 nummod 200,μM
R2570 T3203 T3200 appos μM,MgCl2
R2571 T3204 T3203 prep of,μM
R2572 T3205 T3206 det each,dNTP
R2573 T3206 T3204 pobj dNTP,of
R2574 T3207 T3203 punct ",",μM
R2575 T3208 T3209 nummod 200,μM
R2576 T3209 T3203 conj μM,μM
R2577 T3210 T3209 prep of,μM
R2578 T3211 T3210 pobj primers,of
R2579 T3212 T3209 punct ",",μM
R2580 T3213 T3209 cc and,μM
R2581 T3214 T3217 nummod 2u,polymerase
R2582 T3215 T3217 compound Taq,polymerase
R2583 T3216 T3217 compound DNA,polymerase
R2584 T3217 T3209 conj polymerase,μM
R2585 T3218 T3185 punct .,used
R2586 T3219 T3220 det The,house
R2587 T3220 T3222 compound house,gene
R2588 T3221 T3222 compound keep,gene
R2589 T3222 T3227 nsubjpass gene,used
R2590 T3223 T3222 punct (,gene
R2591 T3224 T3222 appos β-actin,gene
R2592 T3225 T3222 punct ),gene
R2593 T3226 T3227 auxpass was,used
R2594 T3227 T3227 ROOT used,used
R2595 T3228 T3227 prep as,used
R2596 T3229 T3231 det an,control
R2597 T3230 T3231 amod internal,control
R2598 T3231 T3228 pobj control,as
R2599 T3232 T3227 punct .,used
R2600 T3233 T3236 det The,sequences
R2601 T3234 T3235 compound PCR,primer
R2602 T3235 T3236 compound primer,sequences
R2603 T3236 T3238 nsubjpass sequences,designed
R2604 T3237 T3238 auxpass were,designed
R2605 T3238 T3238 ROOT designed,designed
R2606 T3239 T3238 prep according,designed
R2607 T3240 T3239 prep to,according
R2608 T3241 T3246 det the,sequences
R2609 T3242 T3246 nummod TLR4,sequences
R2610 T3243 T3242 cc and,TLR4
R2611 T3244 T3242 conj β-actin,TLR4
R2612 T3245 T3246 compound gene,sequences
R2613 T3246 T3240 pobj sequences,to
R2614 T3247 T3246 acl reported,sequences
R2615 T3248 T3247 prep in,reported
R2616 T3249 T3248 pobj GenBank,in
R2617 T3250 T3238 punct .,designed
R2618 T3251 T3251 ROOT TLR4,TLR4
R2619 T3252 T3254 punct (,bp
R2620 T3253 T3254 nummod 145,bp
R2621 T3254 T3251 appos bp,TLR4
R2622 T3255 T3254 punct ),bp
R2623 T3256 T3254 punct :,bp
R2624 T3257 T3263 advmod forward,′
R2625 T3258 T3259 punct :,5
R2626 T3259 T3263 nummod 5,′
R2627 T3260 T3259 punct ′,5
R2628 T3261 T3262 punct -,AGCTTTGGTCAGTTGGCTCT-3
R2629 T3262 T3263 compound AGCTTTGGTCAGTTGGCTCT-3,′
R2630 T3263 T3254 appos ′,bp
R2631 T3264 T3254 punct ;,bp
R2632 T3265 T3287 ccomp reverse,reverse
R2633 T3266 T3265 punct :,reverse
R2634 T3267 T3271 nummod 5,′
R2635 T3268 T3270 punct ′,CAGGATGACACCATTGAAGC-3
R2636 T3269 T3270 punct -,CAGGATGACACCATTGAAGC-3
R2637 T3270 T3271 compound CAGGATGACACCATTGAAGC-3,′
R2638 T3271 T3265 appos ′,reverse
R2639 T3272 T3287 punct ;,reverse
R2640 T3273 T3287 ccomp β-actin,reverse
R2641 T3274 T3276 punct (,bp
R2642 T3275 T3276 nummod 701,bp
R2643 T3276 T3273 appos bp,β-actin
R2644 T3277 T3273 punct ),β-actin
R2645 T3278 T3273 punct :,β-actin
R2646 T3279 T3273 advmod forward,β-actin
R2647 T3280 T3273 punct :,β-actin
R2648 T3281 T3284 meta 5,GCCAACCGTGAAAAAGATG-3
R2649 T3282 T3281 punct ′,5
R2650 T3283 T3284 punct -,GCCAACCGTGAAAAAGATG-3
R2651 T3284 T3285 compound GCCAACCGTGAAAAAGATG-3,′
R2652 T3285 T3273 appos ′,β-actin
R2653 T3286 T3287 punct ;,reverse
R2654 T3287 T3251 appos reverse,TLR4
R2655 T3288 T3287 punct :,reverse
R2656 T3289 T3292 nummod 5,CCAGGATAGAGCCACCAAT-3
R2657 T3290 T3292 punct ′,CCAGGATAGAGCCACCAAT-3
R2658 T3291 T3292 punct -,CCAGGATAGAGCCACCAAT-3
R2659 T3292 T3293 compound CCAGGATAGAGCCACCAAT-3,′
R2660 T3293 T3287 appos ′,reverse
R2661 T3294 T3287 punct .,reverse
R2662 T3295 T3296 compound Reaction,cycles
R2663 T3296 T3298 nsubjpass cycles,performed
R2664 T3297 T3298 auxpass were,performed
R2665 T3298 T3298 ROOT performed,performed
R2666 T3299 T3298 prep at,performed
R2667 T3300 T3302 det the,condition
R2668 T3301 T3302 amod following,condition
R2669 T3302 T3299 pobj condition,at
R2670 T3303 T3298 punct :,performed
R2671 T3304 T3314 nummod 94,s
R2672 T3305 T3306 compound °,C
R2673 T3306 T3312 nmod C,C
R2674 T3307 T3306 nummod 2,C
R2675 T3308 T3312 nmod min,C
R2676 T3309 T3308 punct ",",min
R2677 T3310 T3311 nummod 94,°
R2678 T3311 T3312 compound °,C
R2679 T3312 T3314 nmod C,s
R2680 T3313 T3312 nummod 40,C
R2681 T3314 T3298 dep s,performed
R2682 T3315 T3314 punct ",",s
R2683 T3316 T3327 nummod 55,s
R2684 T3317 T3318 compound °,C
R2685 T3318 T3327 nmod C,s
R2686 T3319 T3318 nummod 40,C
R2687 T3320 T3318 prep s,C
R2688 T3321 T3318 punct ",",C
R2689 T3322 T3318 cc and,C
R2690 T3323 T3318 conj 72,C
R2691 T3324 T3325 compound °,C
R2692 T3325 T3327 nmod C,s
R2693 T3326 T3325 nummod 60,C
R2694 T3327 T3314 appos s,s
R2695 T3328 T3298 punct .,performed
R2696 T3329 T3330 det The,reaction
R2697 T3330 T3332 nsubjpass reaction,stopped
R2698 T3331 T3332 auxpass was,stopped
R2699 T3332 T3332 ROOT stopped,stopped
R2700 T3333 T3332 prep after,stopped
R2701 T3334 T3335 nummod 31,cycles
R2702 T3335 T3333 pobj cycles,after
R2703 T3336 T3332 punct .,stopped
R2704 T3337 T3338 compound PCR,products
R2705 T3338 T3340 nsubjpass products,electrophoresed
R2706 T3339 T3340 auxpass were,electrophoresed
R2707 T3340 T3340 ROOT electrophoresed,electrophoresed
R2708 T3341 T3340 prep through,electrophoresed
R2709 T3342 T3343 amod agarose,gel
R2710 T3343 T3341 pobj gel,through
R2711 T3344 T3340 punct .,electrophoresed
R2712 T3345 T3346 compound Image,Acquisition
R2713 T3346 T3351 nsubjpass Acquisition,used
R2714 T3347 T3346 cc and,Acquisition
R2715 T3348 T3346 conj Analysis,Acquisition
R2716 T3349 T3346 conj software,Acquisition
R2717 T3350 T3351 auxpass were,used
R2718 T3351 T3351 ROOT used,used
R2719 T3352 T3353 aux to,determine
R2720 T3353 T3351 xcomp determine,used
R2721 T3354 T3355 compound band,densities
R2722 T3355 T3353 dobj densities,determine
R2723 T3356 T3351 punct .,used
R2724 T3357 T3358 det The,abundance
R2725 T3358 T3363 nsubjpass abundance,expressed
R2726 T3359 T3358 prep of,abundance
R2727 T3360 T3361 nummod TLR4,mRNA
R2728 T3361 T3359 pobj mRNA,of
R2729 T3362 T3363 auxpass was,expressed
R2730 T3363 T3363 ROOT expressed,expressed
R2731 T3364 T3363 prep as,expressed
R2732 T3365 T3366 det the,ratio
R2733 T3366 T3364 pobj ratio,as
R2734 T3367 T3366 prep of,ratio
R2735 T3368 T3369 nummod TLR4,mRNA
R2736 T3369 T3367 pobj mRNA,of
R2737 T3370 T3371 aux to,β-actin
R2738 T3371 T3366 relcl β-actin,ratio
R2739 T3372 T3371 dobj mRNA,β-actin
R2740 T3373 T3363 punct .,expressed

bionlp-st-ge-2016-test-tees

Id Subject Object Predicate Lexical cue
T2803 1481-1486 Positive_regulation denotes ratio
T2802 1437-1446 Positive_regulation denotes abundance
T2801 1503-1515 Protein denotes β-actin mRNA
T2800 1490-1499 Protein denotes TLR4 mRNA
T2799 1450-1459 Protein denotes TLR4 mRNA
T2798 1059-1066 Protein denotes β-actin
T2797 961-968 Protein denotes GenBank
T2796 926-933 Protein denotes β-actin
T2795 917-921 Protein denotes TLR4
T2794 819-826 Protein denotes β-actin
T2793 778-796 Protein denotes Taq DNA polymerase
T2792 566-586 Protein denotes RT-PCR (promega Inc)
T2791 5-14 Transcription denotes Detection
T2790 18-27 Protein denotes TLR4 mRNA
R2184 T2790 T2791 themeOf TLR4 mRNA,Detection
R2185 T2799 T2802 themeOf TLR4 mRNA,abundance
R2186 T2800 T2803 themeOf TLR4 mRNA,ratio
R2187 T2801 T2803 causeOf β-actin mRNA,ratio

testone

Id Subject Object Predicate Lexical cue
T2703 1464-1473 Gene_expression denotes expressed
T2702 1503-1510 Protein denotes β-actin
T2701 1490-1494 Protein denotes TLR4
T2700 1450-1454 Protein denotes TLR4
T2699 1059-1066 Protein denotes β-actin
T2698 970-974 Protein denotes TLR4
T2697 926-933 Protein denotes β-actin
T2696 917-921 Protein denotes TLR4
T2695 819-826 Protein denotes β-actin
T2694 798-817 Protein denotes The house keep gene
T2693 778-796 Protein denotes Taq DNA polymerase
T2692 678-682 Protein denotes TLR4
T2691 18-22 Protein denotes TLR4
R2180 T2695 T2694 equivalentTo β-actin,The house keep gene
R2181 T2701 T2703 themeOf TLR4,expressed

test3

Id Subject Object Predicate Lexical cue
T2728 1503-1510 Protein denotes β-actin
T2727 1490-1494 Protein denotes TLR4
T2725 1450-1454 Protein denotes TLR4
T2724 1059-1066 Protein denotes β-actin
T2723 970-974 Protein denotes TLR4
T2722 926-933 Protein denotes β-actin
T2721 917-921 Protein denotes TLR4
T2720 819-826 Protein denotes β-actin
T2719 798-817 Protein denotes The house keep gene
T2718 778-796 Protein denotes Taq DNA polymerase
T2717 678-682 Protein denotes TLR4
T2716 18-22 Protein denotes TLR4
T2715 1503-1510 Protein denotes β-actin
T2714 1490-1494 Protein denotes TLR4
T2713 1450-1454 Protein denotes TLR4
T2712 1059-1066 Protein denotes β-actin
T2711 970-974 Protein denotes TLR4
T2710 926-933 Protein denotes β-actin
T2709 917-921 Protein denotes TLR4
T2708 819-826 Protein denotes β-actin
T2707 798-817 Protein denotes The house keep gene
T2706 778-796 Protein denotes Taq DNA polymerase
T2705 678-682 Protein denotes TLR4
T2704 18-22 Protein denotes TLR4
T2726 1464-1473 Transcription denotes expressed
R2182 T2720 T2719 equivalentTo β-actin,The house keep gene