PMC:2812708 / 7674-8678 JSONTXT

{"project":"MyTest","denotations":[{"id":"19242689-19816525-28778239","span":{"begin":86,"end":90},"obj":"19816525"}],"namespaces":[{"prefix":"_base","uri":"https://www.uniprot.org/uniprot/testbase"},{"prefix":"UniProtKB","uri":"https://www.uniprot.org/uniprot/"},{"prefix":"uniprot","uri":"https://www.uniprot.org/uniprotkb/"}],"text":"A Multiwavelength AUC as described in Bhattacharyya et al. (2006) and Strauss et al. (2008) was used at 25°C. We have applied a band centrifugation experiment using a Vinograd cell. In contrast to conventional sedimentation velocity experiments, where the sample between boundary and bottom of the cell is only diluted by radial dilution, the sample in band centrifugation is diluted additionally by fractionation in the pure solvent. The reservoir is filled with a small amount of concentrated sample. Column and sample sectors are filled with D2O with a density which is higher than that of the sample solution and lower than the density of the dispersed solute. We have prepared a 20 g/l solution and deposited 15 µl of the solution into the cell reservoir. After preliminary experiments, this concentration was chosen to ensure that the individual components of the mixture are detected in as many as possible scans with OD’s \u003c 1.4 in the experiment without too much dilution which causes noisy data."}

{"project":"2_test","denotations":[{"id":"19242689-19816525-28778239","span":{"begin":86,"end":90},"obj":"19816525"}],"text":"A Multiwavelength AUC as described in Bhattacharyya et al. (2006) and Strauss et al. (2008) was used at 25°C. We have applied a band centrifugation experiment using a Vinograd cell. In contrast to conventional sedimentation velocity experiments, where the sample between boundary and bottom of the cell is only diluted by radial dilution, the sample in band centrifugation is diluted additionally by fractionation in the pure solvent. The reservoir is filled with a small amount of concentrated sample. Column and sample sectors are filled with D2O with a density which is higher than that of the sample solution and lower than the density of the dispersed solute. We have prepared a 20 g/l solution and deposited 15 µl of the solution into the cell reservoir. After preliminary experiments, this concentration was chosen to ensure that the individual components of the mixture are detected in as many as possible scans with OD’s \u003c 1.4 in the experiment without too much dilution which causes noisy data."}