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{"project":"sentences","denotations":[{"id":"T26529","span":{"begin":0,"end":608},"obj":"Sentence"},{"id":"T379","span":{"begin":0,"end":608},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Cells were lysed with immunoprecipitation buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 5 mM EDTA, NP-40 [0.5% vol/vol]) containing phosphatase (Roche) and protease inhibitors cocktails (Roche), the nuclear pellet was washed, the chromatin was sheared by sonication and incubated with antibodies for RUNX1 (H-65 X; Santa Cruz Biotechnology, Inc.), RUNX3 (H-50 X; Santa Cruz Biotechnology, Inc.), CBFβ (PEBP2β; FL-182 X; Santa Cruz Biotechnology, Inc.), and as controls normal rabbit IgG (Santa Cruz Biotechnology, Inc.), anti-human RNA polymerase II antibody, and mouse control IgG (both from SA Biosciences)."}

{"project":"events-check-again","denotations":[{"id":"T27046","span":{"begin":299,"end":304},"obj":"Protein"},{"id":"T27047","span":{"begin":347,"end":352},"obj":"Protein"},{"id":"T27048","span":{"begin":395,"end":399},"obj":"Protein"},{"id":"T27049","span":{"begin":531,"end":548},"obj":"Protein"}],"text":"Cells were lysed with immunoprecipitation buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 5 mM EDTA, NP-40 [0.5% vol/vol]) containing phosphatase (Roche) and protease inhibitors cocktails (Roche), the nuclear pellet was washed, the chromatin was sheared by sonication and incubated with antibodies for RUNX1 (H-65 X; Santa Cruz Biotechnology, Inc.), RUNX3 (H-50 X; Santa Cruz Biotechnology, Inc.), CBFβ (PEBP2β; FL-182 X; Santa Cruz Biotechnology, Inc.), and as controls normal rabbit IgG (Santa Cruz Biotechnology, Inc.), anti-human RNA polymerase II antibody, and mouse control IgG (both from SA Biosciences)."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T27007","span":{"begin":299,"end":304},"obj":"Protein"},{"id":"T27008","span":{"begin":347,"end":352},"obj":"Protein"},{"id":"T27009","span":{"begin":395,"end":399},"obj":"Protein"},{"id":"T27010","span":{"begin":401,"end":407},"obj":"Protein"},{"id":"T27011","span":{"begin":482,"end":485},"obj":"Protein"},{"id":"T27012","span":{"begin":520,"end":557},"obj":"Protein"},{"id":"T27013","span":{"begin":563,"end":580},"obj":"Protein"}],"text":"Cells were lysed with immunoprecipitation buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 5 mM EDTA, NP-40 [0.5% vol/vol]) containing phosphatase (Roche) and protease inhibitors cocktails (Roche), the nuclear pellet was washed, the chromatin was sheared by sonication and incubated with antibodies for RUNX1 (H-65 X; Santa Cruz Biotechnology, Inc.), RUNX3 (H-50 X; Santa Cruz Biotechnology, Inc.), CBFβ (PEBP2β; FL-182 X; Santa Cruz Biotechnology, Inc.), and as controls normal rabbit IgG (Santa Cruz Biotechnology, Inc.), anti-human RNA polymerase II antibody, and mouse control IgG (both from SA Biosciences)."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T26513","span":{"begin":299,"end":304},"obj":"Protein"},{"id":"T26514","span":{"begin":347,"end":352},"obj":"Protein"},{"id":"T26515","span":{"begin":395,"end":399},"obj":"Protein"},{"id":"T26516","span":{"begin":531,"end":548},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Cells were lysed with immunoprecipitation buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 5 mM EDTA, NP-40 [0.5% vol/vol]) containing phosphatase (Roche) and protease inhibitors cocktails (Roche), the nuclear pellet was washed, the chromatin was sheared by sonication and incubated with antibodies for RUNX1 (H-65 X; Santa Cruz Biotechnology, Inc.), RUNX3 (H-50 X; Santa Cruz Biotechnology, Inc.), CBFβ (PEBP2β; FL-182 X; Santa Cruz Biotechnology, Inc.), and as controls normal rabbit IgG (Santa Cruz Biotechnology, Inc.), anti-human RNA polymerase II antibody, and mouse control IgG (both from SA Biosciences)."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T26542","span":{"begin":79,"end":81},"obj":"P0A7Z4"},{"id":"T26543","span":{"begin":299,"end":304},"obj":"Q01196"},{"id":"T26544","span":{"begin":347,"end":352},"obj":"Q13761"},{"id":"T26545","span":{"begin":395,"end":399},"obj":"Q13951"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Cells were lysed with immunoprecipitation buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 5 mM EDTA, NP-40 [0.5% vol/vol]) containing phosphatase (Roche) and protease inhibitors cocktails (Roche), the nuclear pellet was washed, the chromatin was sheared by sonication and incubated with antibodies for RUNX1 (H-65 X; Santa Cruz Biotechnology, Inc.), RUNX3 (H-50 X; Santa Cruz Biotechnology, Inc.), CBFβ (PEBP2β; FL-182 X; Santa Cruz Biotechnology, Inc.), and as controls normal rabbit IgG (Santa Cruz Biotechnology, Inc.), anti-human RNA polymerase II antibody, and mouse control IgG (both from SA Biosciences)."}

{"project":"test2","denotations":[{"id":"T26493","span":{"begin":299,"end":304},"obj":"Protein"},{"id":"T26494","span":{"begin":347,"end":352},"obj":"Protein"},{"id":"T26495","span":{"begin":395,"end":399},"obj":"Protein"},{"id":"T26496","span":{"begin":531,"end":548},"obj":"Protein"}],"text":"Cells were lysed with immunoprecipitation buffer (150 mM NaCl, 50 mM Tris-HCl, pH 7.5, 5 mM EDTA, NP-40 [0.5% vol/vol]) containing phosphatase (Roche) and protease inhibitors cocktails (Roche), the nuclear pellet was washed, the chromatin was sheared by sonication and incubated with antibodies for RUNX1 (H-65 X; Santa Cruz Biotechnology, Inc.), RUNX3 (H-50 X; Santa Cruz Biotechnology, Inc.), CBFβ (PEBP2β; FL-182 X; Santa Cruz Biotechnology, Inc.), and as controls normal rabbit IgG (Santa Cruz Biotechnology, Inc.), anti-human RNA polymerase II antibody, and mouse control IgG (both from SA Biosciences)."}