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PMC:2806624 / 51852-53015 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T25371 0-9 JJ denotes Pull-down
T25372 10-15 NN denotes assay
T25373 17-24 NN denotes HEK293T
T25374 25-30 NN denotes cells
T25375 31-35 VB denotes were
T25376 36-47 VB denotes transfected
T25377 48-52 IN denotes with
T25378 53-58 NN denotes RUNX1
T25379 59-61 CC denotes or
T25380 62-67 NN denotes RUNX3
T25381 68-73 VB denotes using
T25382 74-77 DT denotes the
T25383 78-91 NN denotes Lipofectamine
T25384 92-96 CD denotes 2000
T25385 97-104 NN denotes reagent
T25386 105-106 -LRB- denotes (
T25387 106-116 NN denotes Invitrogen
T25388 116-117 -RRB- denotes )
T25389 118-127 VB denotes according
T25390 128-130 TO denotes to
T25391 131-134 DT denotes the
T25392 135-147 NN denotes manufacturer
T25393 147-149 POS denotes 's
T25394 150-161 NN denotes instruction
T25395 163-168 NN denotes Cells
T25396 169-173 VB denotes were
T25397 174-179 VB denotes lysed
T25398 180-182 IN denotes by
T25399 183-193 NN denotes sonication
T25400 194-196 IN denotes in
T25401 197-201 NN denotes HKMG
T25402 202-208 NN denotes buffer
T25403 209-210 -LRB- denotes (
T25404 210-212 CD denotes 10
T25405 213-215 NN denotes mM
T25406 216-221 NNP denotes Hepes
T25407 221-222 -COMMA- denotes ,
T25408 223-225 NN denotes pH
T25409 226-229 CD denotes 7.9
T25410 229-230 -COMMA- denotes ,
T25411 231-234 CD denotes 100
T25412 235-237 NN denotes mM
T25413 238-241 NN denotes KCl
T25414 241-242 -COMMA- denotes ,
T25415 243-244 CD denotes 5
T25416 245-247 NN denotes mM
T25417 248-253 NN denotes MgCl2
T25418 253-254 -COMMA- denotes ,
T25419 255-257 CD denotes 10
T25420 257-258 NN denotes %
T25421 259-267 NN denotes glycerol
T25422 267-268 -COMMA- denotes ,
T25423 269-270 CD denotes 1
T25424 271-273 NN denotes mM
T25425 274-277 NN denotes DTT
T25426 277-278 -COMMA- denotes ,
T25427 279-282 CD denotes 0.5
T25428 282-283 NN denotes %
T25429 284-291 NN denotes Nonidet
T25430 292-296 NN denotes P-40
T25431 296-297 -RRB- denotes )
T25432 298-308 VB denotes containing
T25433 309-310 DT denotes a
T25434 311-319 NN denotes protease
T25435 320-329 NN denotes inhibitor
T25436 330-338 NN denotes cocktail
T25437 339-340 -LRB- denotes (
T25438 340-345 NNP denotes Roche
T25439 346-357 NNP denotes Diagnostics
T25440 357-358 -RRB- denotes )
T25441 360-363 DT denotes The
T25442 364-368 NN denotes cell
T25443 369-375 NN denotes lysate
T25444 376-379 VB denotes was
T25445 380-390 VB denotes precleared
T25446 391-396 VB denotes using
T25447 397-417 JJ denotes streptavidin-agarose
T25448 418-423 NN denotes beads
T25449 424-425 -LRB- denotes (
T25450 425-427 NNP denotes GE
T25451 428-438 NNP denotes Healthcare
T25452 438-439 -RRB- denotes )
T25453 439-440 -COMMA- denotes ,
T25454 441-450 VB denotes incubated
T25455 451-455 IN denotes with
T25456 456-468 VB denotes biotinylated
T25457 469-484 JJ denotes double-stranded
T25458 485-501 NN denotes oligonucleotides
T25459 502-512 VB denotes containing
T25460 513-516 DT denotes the
T25461 517-526 JJ denotes wild-type
T25462 527-529 CC denotes or
T25463 530-537 VB denotes mutated
T25464 538-542 NN denotes RUNX
T25465 543-550 NN denotes binding
T25466 551-556 NN denotes sites
T25467 556-557 -COMMA- denotes ,
T25468 558-561 CC denotes and
T25469 562-593 JJ denotes polydeoxyinosinicdeoxycytidylic
T25470 594-598 NN denotes acid
T25471 599-600 -LRB- denotes (
T25472 600-613 JJ denotes Sigma-Aldrich
T25473 613-614 -RRB- denotes )
T25474 616-617 DT denotes A
T25475 618-629 NN denotes combination
T25476 630-632 IN denotes of
T25477 633-636 DT denotes all
T25478 637-642 CD denotes three
T25479 643-659 NN denotes oligonucleotides
T25480 660-670 VB denotes containing
T25481 671-674 DT denotes the
T25482 675-682 VB denotes mutated
T25483 683-685 CC denotes or
T25484 686-689 DT denotes the
T25485 690-699 JJ denotes wild-type
T25486 700-707 NN denotes binding
T25487 708-713 NN denotes sites
T25488 714-717 VB denotes was
T25489 718-722 VB denotes used
T25490 723-725 IN denotes in
T25491 726-729 DT denotes the
T25492 730-735 NN denotes assay
T25493 737-746 JJ denotes DNA-bound
T25494 747-755 NN denotes proteins
T25495 756-760 VB denotes were
T25496 761-770 VB denotes collected
T25497 771-775 IN denotes with
T25498 776-796 JJ denotes streptavidin-agarose
T25499 797-802 NN denotes beads
T25500 802-803 -COMMA- denotes ,
T25501 804-810 VB denotes washed
T25502 811-815 IN denotes with
T25503 816-820 NN denotes HKMG
T25504 821-827 NN denotes buffer
T25505 827-828 -COMMA- denotes ,
T25506 829-832 CC denotes and
T25507 833-840 RB denotes finally
T25508 841-852 VB denotes resuspended
T25509 853-855 IN denotes in
T25510 856-862 NN denotes NuPAGE
T25511 863-870 NN denotes loading
T25512 871-877 NN denotes buffer
T25513 878-879 -LRB- denotes (
T25514 879-889 NNP denotes Invitrogen
T25515 890-894 NNP denotes Life
T25516 895-907 NNP denotes Technologies
T25517 907-908 -RRB- denotes )
T25518 908-909 -COMMA- denotes ,
T25519 910-916 VB denotes heated
T25520 917-919 TO denotes to
T25521 920-924 NN denotes 70°C
T25522 925-928 IN denotes for
T25523 929-931 CD denotes 10
T25524 932-935 NN denotes min
T25525 935-936 -COMMA- denotes ,
T25526 937-940 CC denotes and
T25527 941-950 VB denotes separated
T25528 951-953 IN denotes on
T25529 954-955 DT denotes a
T25530 956-962 NN denotes NuPAGE
T25531 963-967 CD denotes 4–12
T25532 967-968 NN denotes %
T25533 969-977 NN denotes Bis-Tris
T25534 978-981 NN denotes gel
T25535 982-983 -LRB- denotes (
T25536 983-993 NNP denotes Invitrogen
T25537 994-998 NNP denotes Life
T25538 999-1011 NNP denotes Technologies
T25539 1011-1012 -RRB- denotes )
T25540 1014-1017 DT denotes The
T25541 1018-1026 NN denotes proteins
T25542 1027-1031 VB denotes were
T25543 1032-1046 VB denotes electroblotted
T25544 1047-1051 IN denotes onto
T25545 1052-1053 DT denotes a
T25546 1054-1058 NN denotes PVDF
T25547 1059-1067 NN denotes membrane
T25548 1068-1069 -LRB- denotes (
T25549 1069-1071 NNP denotes GE
T25550 1072-1082 NNP denotes Healthcare
T25551 1082-1083 -RRB- denotes )
T25552 1084-1087 CC denotes and
T25553 1088-1096 VB denotes detected
T25554 1097-1102 VB denotes using
T25555 1103-1108 NN denotes RUNX1
T25556 1109-1111 CC denotes or
T25557 1112-1117 NN denotes RUNX3
T25558 1118-1128 NN denotes antibodies
T25559 1129-1138 VB denotes described
T25560 1139-1141 IN denotes in
T25561 1142-1145 DT denotes the
T25562 1146-1154 JJ denotes previous
T25563 1155-1162 NN denotes section
R19716 T25372 T25371 arg1Of assay,Pull-down
R19717 T25374 T25373 arg1Of cells,HEK293T
R19718 T25374 T25375 arg1Of cells,were
R19719 T25374 T25376 arg2Of cells,transfected
R19720 T25376 T25375 arg2Of transfected,were
R19721 T25376 T25377 arg1Of transfected,with
R19722 T25376 T25389 arg1Of transfected,according
R19723 T25378 T25379 arg1Of RUNX1,or
R19724 T25379 T25377 arg2Of or,with
R19725 T25379 T25381 arg1Of or,using
R19726 T25380 T25379 arg2Of RUNX3,or
R19727 T25385 T25381 arg2Of reagent,using
R19728 T25385 T25382 arg1Of reagent,the
R19729 T25385 T25383 arg1Of reagent,Lipofectamine
R19730 T25385 T25384 arg1Of reagent,2000
R19731 T25385 T25386 arg1Of reagent,(
R19732 T25387 T25386 arg2Of Invitrogen,(
R19733 T25388 T25386 arg3Of ),(
R19734 T25390 T25389 arg2Of to,according
R19735 T25392 T25391 arg1Of manufacturer,the
R19736 T25392 T25393 arg2Of manufacturer,'s
R19737 T25394 T25390 arg2Of instruction,to
R19738 T25394 T25393 arg1Of instruction,'s
R19739 T25395 T25396 arg1Of Cells,were
R19740 T25395 T25397 arg2Of Cells,lysed
R19741 T25397 T25396 arg2Of lysed,were
R19742 T25399 T25397 arg1Of sonication,lysed
R19743 T25399 T25398 arg2Of sonication,by
R19744 T25399 T25400 arg1Of sonication,in
R19745 T25402 T25400 arg2Of buffer,in
R19746 T25402 T25401 arg1Of buffer,HKMG
R19747 T25402 T25403 arg1Of buffer,(
R19748 T25402 T25432 arg1Of buffer,containing
R19749 T25406 T25403 arg2Of Hepes,(
R19750 T25406 T25404 arg1Of Hepes,10
R19751 T25406 T25405 arg1Of Hepes,mM
R19752 T25406 T25407 arg1Of Hepes,","
R19753 T25406 T25426 arg1Of Hepes,","
R19754 T25408 T25409 arg1Of pH,7.9
R19755 T25408 T25410 arg1Of pH,","
R19756 T25410 T25414 arg1Of ",",","
R19757 T25411 T25412 arg1Of 100,mM
R19758 T25413 T25410 arg2Of KCl,","
R19759 T25413 T25411 arg1Of KCl,100
R19760 T25414 T25418 arg1Of ",",","
R19761 T25415 T25416 arg1Of 5,mM
R19762 T25417 T25414 arg2Of MgCl2,","
R19763 T25417 T25415 arg1Of MgCl2,5
R19764 T25418 T25422 arg1Of ",",","
R19765 T25419 T25420 arg1Of 10,%
R19766 T25421 T25418 arg2Of glycerol,","
R19767 T25421 T25419 arg1Of glycerol,10
R19768 T25422 T25407 arg2Of ",",","
R19769 T25423 T25424 arg1Of 1,mM
R19770 T25425 T25422 arg2Of DTT,","
R19771 T25425 T25423 arg1Of DTT,1
R19772 T25427 T25428 arg1Of 0.5,%
R19773 T25430 T25426 arg2Of P-40,","
R19774 T25430 T25427 arg1Of P-40,0.5
R19775 T25430 T25429 arg1Of P-40,Nonidet
R19776 T25431 T25403 arg3Of ),(
R19777 T25436 T25432 arg2Of cocktail,containing
R19778 T25436 T25433 arg1Of cocktail,a
R19779 T25436 T25434 arg1Of cocktail,protease
R19780 T25436 T25435 arg1Of cocktail,inhibitor
R19781 T25436 T25437 arg1Of cocktail,(
R19782 T25439 T25437 arg2Of Diagnostics,(
R19783 T25439 T25438 arg1Of Diagnostics,Roche
R19784 T25440 T25437 arg3Of ),(
R19785 T25443 T25441 arg1Of lysate,The
R19786 T25443 T25442 arg1Of lysate,cell
R19787 T25443 T25444 arg1Of lysate,was
R19788 T25443 T25445 arg2Of lysate,precleared
R19789 T25443 T25446 arg1Of lysate,using
R19790 T25445 T25444 arg2Of precleared,was
R19791 T25446 T25445 arg3Of using,precleared
R19792 T25448 T25447 arg1Of beads,streptavidin-agarose
R19793 T25448 T25449 arg1Of beads,(
R19794 T25448 T25453 arg1Of beads,","
R19795 T25448 T25454 arg2Of beads,incubated
R19796 T25448 T25468 arg1Of beads,and
R19797 T25451 T25449 arg2Of Healthcare,(
R19798 T25451 T25450 arg1Of Healthcare,GE
R19799 T25452 T25449 arg3Of ),(
R19800 T25454 T25455 arg1Of incubated,with
R19801 T25458 T25456 arg2Of oligonucleotides,biotinylated
R19802 T25458 T25457 arg1Of oligonucleotides,double-stranded
R19803 T25458 T25459 arg1Of oligonucleotides,containing
R19804 T25458 T25462 arg1Of oligonucleotides,or
R19805 T25461 T25459 arg2Of wild-type,containing
R19806 T25461 T25460 arg1Of wild-type,the
R19807 T25462 T25455 arg2Of or,with
R19808 T25466 T25462 arg2Of sites,or
R19809 T25466 T25463 arg2Of sites,mutated
R19810 T25466 T25464 arg1Of sites,RUNX
R19811 T25466 T25465 arg1Of sites,binding
R19812 T25468 T25446 arg2Of and,using
R19813 T25468 T25467 arg1Of and,","
R19814 T25470 T25468 arg2Of acid,and
R19815 T25470 T25469 arg1Of acid,polydeoxyinosinicdeoxycytidylic
R19816 T25470 T25471 arg1Of acid,(
R19817 T25472 T25471 arg2Of Sigma-Aldrich,(
R19818 T25473 T25471 arg3Of ),(
R19819 T25475 T25474 arg1Of combination,A
R19820 T25475 T25476 arg1Of combination,of
R19821 T25475 T25488 arg1Of combination,was
R19822 T25475 T25489 arg2Of combination,used
R19823 T25479 T25476 arg2Of oligonucleotides,of
R19824 T25479 T25477 arg1Of oligonucleotides,all
R19825 T25479 T25478 arg1Of oligonucleotides,three
R19826 T25479 T25480 arg1Of oligonucleotides,containing
R19827 T25482 T25483 arg1Of mutated,or
R19828 T25485 T25483 arg2Of wild-type,or
R19829 T25485 T25484 arg1Of wild-type,the
R19830 T25487 T25480 arg2Of sites,containing
R19831 T25487 T25481 arg1Of sites,the
R19832 T25487 T25482 arg1Of sites,mutated
R19833 T25487 T25485 arg1Of sites,wild-type
R19834 T25487 T25486 arg1Of sites,binding
R19835 T25489 T25488 arg2Of used,was
R19836 T25489 T25490 arg1Of used,in
R19837 T25492 T25490 arg2Of assay,in
R19838 T25492 T25491 arg1Of assay,the
R19839 T25494 T25493 arg1Of proteins,DNA-bound
R19840 T25494 T25495 arg1Of proteins,were
R19841 T25494 T25496 arg2Of proteins,collected
R19842 T25494 T25508 arg2Of proteins,resuspended
R19843 T25494 T25527 arg2Of proteins,separated
R19844 T25496 T25497 arg1Of collected,with
R19845 T25496 T25506 arg1Of collected,and
R19846 T25499 T25497 arg2Of beads,with
R19847 T25499 T25498 arg1Of beads,streptavidin-agarose
R19848 T25499 T25500 arg1Of beads,","
R19849 T25499 T25501 arg2Of beads,washed
R19850 T25501 T25502 arg1Of washed,with
R19851 T25504 T25502 arg2Of buffer,with
R19852 T25504 T25503 arg1Of buffer,HKMG
R19853 T25506 T25495 arg2Of and,were
R19854 T25506 T25505 arg1Of and,","
R19855 T25508 T25507 arg1Of resuspended,finally
R19856 T25508 T25509 arg1Of resuspended,in
R19857 T25508 T25526 arg1Of resuspended,and
R19858 T25512 T25509 arg2Of buffer,in
R19859 T25512 T25510 arg1Of buffer,NuPAGE
R19860 T25512 T25511 arg1Of buffer,loading
R19861 T25512 T25513 arg1Of buffer,(
R19862 T25512 T25518 arg1Of buffer,","
R19863 T25512 T25519 arg2Of buffer,heated
R19864 T25516 T25513 arg2Of Technologies,(
R19865 T25516 T25514 arg1Of Technologies,Invitrogen
R19866 T25516 T25515 arg1Of Technologies,Life
R19867 T25517 T25513 arg3Of ),(
R19868 T25519 T25520 arg1Of heated,to
R19869 T25519 T25522 arg1Of heated,for
R19870 T25521 T25520 arg2Of 70°C,to
R19871 T25524 T25522 arg2Of min,for
R19872 T25524 T25523 arg1Of min,10
R19873 T25526 T25506 arg2Of and,and
R19874 T25526 T25525 arg1Of and,","
R19875 T25527 T25526 arg2Of separated,and
R19876 T25527 T25528 arg1Of separated,on
R19877 T25531 T25532 arg1Of 4–12,%
R19878 T25534 T25528 arg2Of gel,on
R19879 T25534 T25529 arg1Of gel,a
R19880 T25534 T25530 arg1Of gel,NuPAGE
R19881 T25534 T25531 arg1Of gel,4–12
R19882 T25534 T25533 arg1Of gel,Bis-Tris
R19883 T25534 T25535 arg1Of gel,(
R19884 T25538 T25535 arg2Of Technologies,(
R19885 T25538 T25536 arg1Of Technologies,Invitrogen
R19886 T25538 T25537 arg1Of Technologies,Life
R19887 T25539 T25535 arg3Of ),(
R19888 T25541 T25540 arg1Of proteins,The
R19889 T25541 T25542 arg1Of proteins,were
R19890 T25541 T25543 arg2Of proteins,electroblotted
R19891 T25541 T25553 arg2Of proteins,detected
R19892 T25541 T25554 arg1Of proteins,using
R19893 T25543 T25544 arg1Of electroblotted,onto
R19894 T25543 T25552 arg1Of electroblotted,and
R19895 T25547 T25544 arg2Of membrane,onto
R19896 T25547 T25545 arg1Of membrane,a
R19897 T25547 T25546 arg1Of membrane,PVDF
R19898 T25547 T25548 arg1Of membrane,(
R19899 T25550 T25548 arg2Of Healthcare,(
R19900 T25550 T25549 arg1Of Healthcare,GE
R19901 T25551 T25548 arg3Of ),(
R19902 T25552 T25542 arg2Of and,were
R19903 T25553 T25552 arg2Of detected,and
R19904 T25554 T25553 arg3Of using,detected
R19905 T25555 T25556 arg1Of RUNX1,or
R19906 T25556 T25554 arg2Of or,using
R19907 T25558 T25556 arg2Of antibodies,or
R19908 T25558 T25557 arg1Of antibodies,RUNX3
R19909 T25558 T25559 arg2Of antibodies,described
R19910 T25559 T25560 arg1Of described,in
R19911 T25563 T25560 arg2Of section,in
R19912 T25563 T25561 arg1Of section,the
R19913 T25563 T25562 arg1Of section,previous

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T25579 0-9 JJ denotes Pull-down
T25580 10-15 NN denotes assay
T25581 15-16 . denotes .
T25582 17-24 CD denotes HEK293T
T25583 25-30 NNS denotes cells
T25584 31-35 VBD denotes were
T25585 36-47 VBN denotes transfected
T25586 48-52 IN denotes with
T25587 53-58 CD denotes RUNX1
T25588 59-61 CC denotes or
T25589 62-67 CD denotes RUNX3
T25590 68-73 VBG denotes using
T25591 74-77 DT denotes the
T25592 78-91 NNP denotes Lipofectamine
T25593 92-96 CD denotes 2000
T25594 97-104 NN denotes reagent
T25595 105-106 -LRB- denotes (
T25596 106-116 NNP denotes Invitrogen
T25597 116-117 -RRB- denotes )
T25598 118-127 VBG denotes according
T25599 128-130 TO denotes to
T25600 131-134 DT denotes the
T25601 135-147 NN denotes manufacturer
T25602 147-149 POS denotes 's
T25603 150-161 NN denotes instruction
T25604 161-162 . denotes .
T25605 163-168 NNS denotes Cells
T25606 169-173 VBD denotes were
T25607 174-179 VBN denotes lysed
T25608 180-182 IN denotes by
T25609 183-193 NN denotes sonication
T25610 194-196 IN denotes in
T25611 197-201 NNP denotes HKMG
T25612 202-208 NN denotes buffer
T25613 209-210 -LRB- denotes (
T25614 210-212 CD denotes 10
T25615 213-215 NNP denotes mM
T25616 216-221 NNP denotes Hepes
T25617 221-222 , denotes ,
T25618 223-225 NNP denotes pH
T25619 226-229 CD denotes 7.9
T25620 229-230 , denotes ,
T25621 231-234 CD denotes 100
T25622 235-237 NNP denotes mM
T25623 238-241 NNP denotes KCl
T25624 241-242 , denotes ,
T25625 243-244 CD denotes 5
T25626 245-247 NNP denotes mM
T25627 248-253 NNP denotes MgCl2
T25628 253-254 , denotes ,
T25629 255-257 CD denotes 10
T25630 257-258 NN denotes %
T25631 259-267 NN denotes glycerol
T25632 267-268 , denotes ,
T25633 269-270 CD denotes 1
T25634 271-273 NNP denotes mM
T25635 274-277 NNP denotes DTT
T25636 277-278 , denotes ,
T25637 279-282 CD denotes 0.5
T25638 282-283 NN denotes %
T25639 284-291 NNP denotes Nonidet
T25640 292-296 NNP denotes P-40
T25641 296-297 -RRB- denotes )
T25642 298-308 VBG denotes containing
T25643 309-310 DT denotes a
T25644 311-319 NN denotes protease
T25645 320-329 NN denotes inhibitor
T25646 330-338 NN denotes cocktail
T25647 339-340 -LRB- denotes (
T25648 340-345 NNP denotes Roche
T25649 346-357 NNPS denotes Diagnostics
T25650 357-358 -RRB- denotes )
T25651 358-359 . denotes .
T25652 360-363 DT denotes The
T25653 364-368 NN denotes cell
T25654 369-375 NN denotes lysate
T25655 376-379 VBD denotes was
T25656 380-390 VBN denotes precleared
T25657 391-396 VBG denotes using
T25658 397-417 JJ denotes streptavidin-agarose
T25659 418-423 NNS denotes beads
T25660 424-425 -LRB- denotes (
T25661 425-427 NNP denotes GE
T25662 428-438 NNP denotes Healthcare
T25663 438-439 -RRB- denotes )
T25664 439-440 , denotes ,
T25665 441-450 VBN denotes incubated
T25666 451-455 IN denotes with
T25667 456-468 JJ denotes biotinylated
T25668 469-484 JJ denotes double-stranded
T25669 485-501 NNS denotes oligonucleotides
T25670 502-512 VBG denotes containing
T25671 513-516 DT denotes the
T25672 517-526 JJ denotes wild-type
T25673 527-529 CC denotes or
T25674 530-537 VBN denotes mutated
T25675 538-542 NNP denotes RUNX
T25676 543-550 JJ denotes binding
T25677 551-556 NNS denotes sites
T25678 556-557 , denotes ,
T25679 558-561 CC denotes and
T25680 562-593 JJ denotes polydeoxyinosinicdeoxycytidylic
T25681 594-598 NN denotes acid
T25682 599-600 -LRB- denotes (
T25683 600-613 NNP denotes Sigma-Aldrich
T25684 613-614 -RRB- denotes )
T25685 614-615 . denotes .
T25686 616-617 DT denotes A
T25687 618-629 NN denotes combination
T25688 630-632 IN denotes of
T25689 633-636 DT denotes all
T25690 637-642 CD denotes three
T25691 643-659 NNS denotes oligonucleotides
T25692 660-670 VBG denotes containing
T25693 671-674 DT denotes the
T25694 675-682 VBN denotes mutated
T25695 683-685 CC denotes or
T25696 686-689 DT denotes the
T25697 690-699 JJ denotes wild-type
T25698 700-707 JJ denotes binding
T25699 708-713 NNS denotes sites
T25700 714-717 VBD denotes was
T25701 718-722 VBN denotes used
T25702 723-725 IN denotes in
T25703 726-729 DT denotes the
T25704 730-735 NN denotes assay
T25705 735-736 . denotes .
T25706 737-746 JJ denotes DNA-bound
T25707 747-755 NNS denotes proteins
T25708 756-760 VBD denotes were
T25709 761-770 VBN denotes collected
T25710 771-775 IN denotes with
T25711 776-796 JJ denotes streptavidin-agarose
T25712 797-802 NNS denotes beads
T25713 802-803 , denotes ,
T25714 804-810 VBN denotes washed
T25715 811-815 IN denotes with
T25716 816-820 NNP denotes HKMG
T25717 821-827 NN denotes buffer
T25718 827-828 , denotes ,
T25719 829-832 CC denotes and
T25720 833-840 RB denotes finally
T25721 841-852 VBD denotes resuspended
T25722 853-855 IN denotes in
T25723 856-862 NNP denotes NuPAGE
T25724 863-870 VBG denotes loading
T25725 871-877 NN denotes buffer
T25726 878-879 -LRB- denotes (
T25727 879-889 NNP denotes Invitrogen
T25728 890-894 NNP denotes Life
T25729 895-907 NNPS denotes Technologies
T25730 907-908 -RRB- denotes )
T25731 908-909 , denotes ,
T25732 910-916 VBN denotes heated
T25733 917-919 TO denotes to
T25734 920-922 CD denotes 70
T25735 922-923 CD denotes °
T25736 923-924 NNP denotes C
T25737 925-928 IN denotes for
T25738 929-931 CD denotes 10
T25739 932-935 NN denotes min
T25740 935-936 , denotes ,
T25741 937-940 CC denotes and
T25742 941-950 VBD denotes separated
T25743 951-953 IN denotes on
T25744 954-955 DT denotes a
T25745 956-962 NNP denotes NuPAGE
T25746 963-964 CD denotes 4
T25747 965-967 CD denotes 12
T25748 967-968 NN denotes %
T25749 969-977 NNP denotes Bis-Tris
T25750 978-981 NN denotes gel
T25751 982-983 -LRB- denotes (
T25752 983-993 NNP denotes Invitrogen
T25753 994-998 NNP denotes Life
T25754 999-1011 NNPS denotes Technologies
T25755 1011-1012 -RRB- denotes )
T25756 1012-1013 . denotes .
T25757 1014-1017 DT denotes The
T25758 1018-1026 NNS denotes proteins
T25759 1027-1031 VBD denotes were
T25760 1032-1046 VBN denotes electroblotted
T25761 1047-1051 IN denotes onto
T25762 1052-1053 DT denotes a
T25763 1054-1058 NNP denotes PVDF
T25764 1059-1067 NN denotes membrane
T25765 1068-1069 -LRB- denotes (
T25766 1069-1071 NNP denotes GE
T25767 1072-1082 NNP denotes Healthcare
T25768 1082-1083 -RRB- denotes )
T25769 1084-1087 CC denotes and
T25770 1088-1096 VBD denotes detected
T25771 1097-1102 VBG denotes using
T25772 1103-1108 CD denotes RUNX1
T25773 1109-1111 CC denotes or
T25774 1112-1117 CD denotes RUNX3
T25775 1118-1128 NNS denotes antibodies
T25776 1129-1138 VBN denotes described
T25777 1139-1141 IN denotes in
T25778 1142-1145 DT denotes the
T25779 1146-1154 JJ denotes previous
T25780 1155-1162 NN denotes section
T25781 1162-1163 . denotes .
R19914 T25579 T25580 amod Pull-down,assay
R19915 T25580 T25580 ROOT assay,assay
R19916 T25581 T25580 punct .,assay
R19917 T25582 T25583 nummod HEK293T,cells
R19918 T25583 T25585 nsubjpass cells,transfected
R19919 T25584 T25585 auxpass were,transfected
R19920 T25585 T25585 ROOT transfected,transfected
R19921 T25586 T25585 prep with,transfected
R19922 T25587 T25590 nsubj RUNX1,using
R19923 T25588 T25587 cc or,RUNX1
R19924 T25589 T25587 conj RUNX3,RUNX1
R19925 T25590 T25586 pcomp using,with
R19926 T25591 T25592 det the,Lipofectamine
R19927 T25592 T25594 nmod Lipofectamine,reagent
R19928 T25593 T25594 nummod 2000,reagent
R19929 T25594 T25590 dobj reagent,using
R19930 T25595 T25594 punct (,reagent
R19931 T25596 T25594 appos Invitrogen,reagent
R19932 T25597 T25594 punct ),reagent
R19933 T25598 T25585 prep according,transfected
R19934 T25599 T25598 prep to,according
R19935 T25600 T25601 det the,manufacturer
R19936 T25601 T25603 poss manufacturer,instruction
R19937 T25602 T25601 case 's,manufacturer
R19938 T25603 T25599 pobj instruction,to
R19939 T25604 T25585 punct .,transfected
R19940 T25605 T25607 nsubjpass Cells,lysed
R19941 T25606 T25607 auxpass were,lysed
R19942 T25607 T25607 ROOT lysed,lysed
R19943 T25608 T25607 agent by,lysed
R19944 T25609 T25608 pobj sonication,by
R19945 T25610 T25609 prep in,sonication
R19946 T25611 T25612 compound HKMG,buffer
R19947 T25612 T25610 pobj buffer,in
R19948 T25613 T25616 punct (,Hepes
R19949 T25614 T25616 nummod 10,Hepes
R19950 T25615 T25616 compound mM,Hepes
R19951 T25616 T25609 appos Hepes,sonication
R19952 T25617 T25616 punct ",",Hepes
R19953 T25618 T25616 appos pH,Hepes
R19954 T25619 T25618 nummod 7.9,pH
R19955 T25620 T25618 punct ",",pH
R19956 T25621 T25623 nummod 100,KCl
R19957 T25622 T25623 compound mM,KCl
R19958 T25623 T25618 conj KCl,pH
R19959 T25624 T25623 punct ",",KCl
R19960 T25625 T25627 nummod 5,MgCl2
R19961 T25626 T25627 compound mM,MgCl2
R19962 T25627 T25623 conj MgCl2,KCl
R19963 T25628 T25627 punct ",",MgCl2
R19964 T25629 T25630 nummod 10,%
R19965 T25630 T25631 compound %,glycerol
R19966 T25631 T25627 conj glycerol,MgCl2
R19967 T25632 T25631 punct ",",glycerol
R19968 T25633 T25635 nummod 1,DTT
R19969 T25634 T25635 compound mM,DTT
R19970 T25635 T25631 appos DTT,glycerol
R19971 T25636 T25635 punct ",",DTT
R19972 T25637 T25638 nummod 0.5,%
R19973 T25638 T25640 compound %,P-40
R19974 T25639 T25640 compound Nonidet,P-40
R19975 T25640 T25635 appos P-40,DTT
R19976 T25641 T25635 punct ),DTT
R19977 T25642 T25618 acl containing,pH
R19978 T25643 T25646 det a,cocktail
R19979 T25644 T25645 amod protease,inhibitor
R19980 T25645 T25646 compound inhibitor,cocktail
R19981 T25646 T25642 dobj cocktail,containing
R19982 T25647 T25649 punct (,Diagnostics
R19983 T25648 T25649 compound Roche,Diagnostics
R19984 T25649 T25646 appos Diagnostics,cocktail
R19985 T25650 T25649 punct ),Diagnostics
R19986 T25651 T25607 punct .,lysed
R19987 T25652 T25654 det The,lysate
R19988 T25653 T25654 compound cell,lysate
R19989 T25654 T25656 nsubjpass lysate,precleared
R19990 T25655 T25656 auxpass was,precleared
R19991 T25656 T25656 ROOT precleared,precleared
R19992 T25657 T25656 advcl using,precleared
R19993 T25658 T25659 amod streptavidin-agarose,beads
R19994 T25659 T25657 dobj beads,using
R19995 T25660 T25659 punct (,beads
R19996 T25661 T25662 compound GE,Healthcare
R19997 T25662 T25659 appos Healthcare,beads
R19998 T25663 T25659 punct ),beads
R19999 T25664 T25656 punct ",",precleared
R20000 T25665 T25656 advcl incubated,precleared
R20001 T25666 T25665 prep with,incubated
R20002 T25667 T25669 amod biotinylated,oligonucleotides
R20003 T25668 T25669 amod double-stranded,oligonucleotides
R20004 T25669 T25666 pobj oligonucleotides,with
R20005 T25670 T25669 prep containing,oligonucleotides
R20006 T25671 T25672 det the,wild-type
R20007 T25672 T25670 dobj wild-type,containing
R20008 T25673 T25672 cc or,wild-type
R20009 T25674 T25672 conj mutated,wild-type
R20010 T25675 T25677 nmod RUNX,sites
R20011 T25676 T25677 amod binding,sites
R20012 T25677 T25674 dobj sites,mutated
R20013 T25678 T25677 punct ",",sites
R20014 T25679 T25677 cc and,sites
R20015 T25680 T25681 amod polydeoxyinosinicdeoxycytidylic,acid
R20016 T25681 T25677 conj acid,sites
R20017 T25682 T25683 punct (,Sigma-Aldrich
R20018 T25683 T25681 appos Sigma-Aldrich,acid
R20019 T25684 T25672 punct ),wild-type
R20020 T25685 T25656 punct .,precleared
R20021 T25686 T25687 det A,combination
R20022 T25687 T25687 ROOT combination,combination
R20023 T25688 T25687 prep of,combination
R20024 T25689 T25691 det all,oligonucleotides
R20025 T25690 T25691 nummod three,oligonucleotides
R20026 T25691 T25688 pobj oligonucleotides,of
R20027 T25692 T25691 acl containing,oligonucleotides
R20028 T25693 T25694 det the,mutated
R20029 T25694 T25692 dobj mutated,containing
R20030 T25695 T25694 cc or,mutated
R20031 T25696 T25699 det the,sites
R20032 T25697 T25699 amod wild-type,sites
R20033 T25698 T25699 amod binding,sites
R20034 T25699 T25701 nsubjpass sites,used
R20035 T25700 T25701 auxpass was,used
R20036 T25701 T25694 conj used,mutated
R20037 T25702 T25701 prep in,used
R20038 T25703 T25704 det the,assay
R20039 T25704 T25702 pobj assay,in
R20040 T25705 T25687 punct .,combination
R20041 T25706 T25707 amod DNA-bound,proteins
R20042 T25707 T25709 nsubjpass proteins,collected
R20043 T25708 T25709 auxpass were,collected
R20044 T25709 T25709 ROOT collected,collected
R20045 T25710 T25709 prep with,collected
R20046 T25711 T25712 amod streptavidin-agarose,beads
R20047 T25712 T25710 pobj beads,with
R20048 T25713 T25712 punct ",",beads
R20049 T25714 T25712 acl washed,beads
R20050 T25715 T25714 prep with,washed
R20051 T25716 T25717 compound HKMG,buffer
R20052 T25717 T25715 pobj buffer,with
R20053 T25718 T25709 punct ",",collected
R20054 T25719 T25709 cc and,collected
R20055 T25720 T25721 advmod finally,resuspended
R20056 T25721 T25709 conj resuspended,collected
R20057 T25722 T25721 prep in,resuspended
R20058 T25723 T25725 nmod NuPAGE,buffer
R20059 T25724 T25725 compound loading,buffer
R20060 T25725 T25722 pobj buffer,in
R20061 T25726 T25729 punct (,Technologies
R20062 T25727 T25729 compound Invitrogen,Technologies
R20063 T25728 T25729 compound Life,Technologies
R20064 T25729 T25725 appos Technologies,buffer
R20065 T25730 T25725 punct ),buffer
R20066 T25731 T25721 punct ",",resuspended
R20067 T25732 T25709 conj heated,collected
R20068 T25733 T25732 prep to,heated
R20069 T25734 T25735 compound 70,°
R20070 T25735 T25736 compound °,C
R20071 T25736 T25733 pobj C,to
R20072 T25737 T25732 prep for,heated
R20073 T25738 T25739 nummod 10,min
R20074 T25739 T25737 pobj min,for
R20075 T25740 T25732 punct ",",heated
R20076 T25741 T25732 cc and,heated
R20077 T25742 T25732 conj separated,heated
R20078 T25743 T25742 prep on,separated
R20079 T25744 T25750 det a,gel
R20080 T25745 T25750 nmod NuPAGE,gel
R20081 T25746 T25745 nummod 4,NuPAGE
R20082 T25747 T25748 nummod 12,%
R20083 T25748 T25750 compound %,gel
R20084 T25749 T25750 compound Bis-Tris,gel
R20085 T25750 T25743 pobj gel,on
R20086 T25751 T25754 punct (,Technologies
R20087 T25752 T25754 compound Invitrogen,Technologies
R20088 T25753 T25754 compound Life,Technologies
R20089 T25754 T25750 appos Technologies,gel
R20090 T25755 T25754 punct ),Technologies
R20091 T25756 T25709 punct .,collected
R20092 T25757 T25758 det The,proteins
R20093 T25758 T25760 nsubjpass proteins,electroblotted
R20094 T25759 T25760 auxpass were,electroblotted
R20095 T25760 T25760 ROOT electroblotted,electroblotted
R20096 T25761 T25760 prep onto,electroblotted
R20097 T25762 T25764 det a,membrane
R20098 T25763 T25764 compound PVDF,membrane
R20099 T25764 T25761 pobj membrane,onto
R20100 T25765 T25767 punct (,Healthcare
R20101 T25766 T25767 compound GE,Healthcare
R20102 T25767 T25764 appos Healthcare,membrane
R20103 T25768 T25767 punct ),Healthcare
R20104 T25769 T25760 cc and,electroblotted
R20105 T25770 T25760 conj detected,electroblotted
R20106 T25771 T25770 xcomp using,detected
R20107 T25772 T25775 nummod RUNX1,antibodies
R20108 T25773 T25772 cc or,RUNX1
R20109 T25774 T25775 nummod RUNX3,antibodies
R20110 T25775 T25771 dobj antibodies,using
R20111 T25776 T25775 acl described,antibodies
R20112 T25777 T25776 prep in,described
R20113 T25778 T25780 det the,section
R20114 T25779 T25780 amod previous,section
R20115 T25780 T25777 pobj section,in
R20116 T25781 T25760 punct .,electroblotted

GO-MF

Id Subject Object Predicate Lexical cue
T25782 543-550 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T25783 700-707 http://purl.obolibrary.org/obo/GO_0005488 denotes binding
T25784 1118-1128 http://purl.obolibrary.org/obo/GO_0003823 denotes antibodies

GO-CC

Id Subject Object Predicate Lexical cue
T25785 25-30 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T25786 364-368 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T25787 1059-1067 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T25788 1118-1128 http://purl.obolibrary.org/obo/GO_0019815 denotes antibodies
T25789 1118-1128 http://purl.obolibrary.org/obo/GO_0042571 denotes antibodies

sentences

Id Subject Object Predicate Lexical cue
T25364 0-16 Sentence denotes Pull-down assay.
T25365 17-162 Sentence denotes HEK293T cells were transfected with RUNX1 or RUNX3 using the Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's instruction.
T25366 163-359 Sentence denotes Cells were lysed by sonication in HKMG buffer (10 mM Hepes, pH 7.9, 100 mM KCl, 5 mM MgCl2, 10% glycerol, 1 mM DTT, 0.5% Nonidet P-40) containing a protease inhibitor cocktail (Roche Diagnostics).
T25367 360-615 Sentence denotes The cell lysate was precleared using streptavidin-agarose beads (GE Healthcare), incubated with biotinylated double-stranded oligonucleotides containing the wild-type or mutated RUNX binding sites, and polydeoxyinosinicdeoxycytidylic acid (Sigma-Aldrich).
T25368 616-736 Sentence denotes A combination of all three oligonucleotides containing the mutated or the wild-type binding sites was used in the assay.
T25369 737-1013 Sentence denotes DNA-bound proteins were collected with streptavidin-agarose beads, washed with HKMG buffer, and finally resuspended in NuPAGE loading buffer (Invitrogen Life Technologies), heated to 70°C for 10 min, and separated on a NuPAGE 4–12% Bis-Tris gel (Invitrogen Life Technologies).
T25370 1014-1163 Sentence denotes The proteins were electroblotted onto a PVDF membrane (GE Healthcare) and detected using RUNX1 or RUNX3 antibodies described in the previous section.
T355 0-16 Sentence denotes Pull-down assay.
T356 17-162 Sentence denotes HEK293T cells were transfected with RUNX1 or RUNX3 using the Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer's instruction.
T357 163-359 Sentence denotes Cells were lysed by sonication in HKMG buffer (10 mM Hepes, pH 7.9, 100 mM KCl, 5 mM MgCl2, 10% glycerol, 1 mM DTT, 0.5% Nonidet P-40) containing a protease inhibitor cocktail (Roche Diagnostics).
T358 360-615 Sentence denotes The cell lysate was precleared using streptavidin-agarose beads (GE Healthcare), incubated with biotinylated double-stranded oligonucleotides containing the wild-type or mutated RUNX binding sites, and polydeoxyinosinicdeoxycytidylic acid (Sigma-Aldrich).
T359 616-736 Sentence denotes A combination of all three oligonucleotides containing the mutated or the wild-type binding sites was used in the assay.
T360 737-1013 Sentence denotes DNA-bound proteins were collected with streptavidin-agarose beads, washed with HKMG buffer, and finally resuspended in NuPAGE loading buffer (Invitrogen Life Technologies), heated to 70°C for 10 min, and separated on a NuPAGE 4–12% Bis-Tris gel (Invitrogen Life Technologies).
T361 1014-1163 Sentence denotes The proteins were electroblotted onto a PVDF membrane (GE Healthcare) and detected using RUNX1 or RUNX3 antibodies described in the previous section.

events-check-again

Id Subject Object Predicate Lexical cue
T25810 36-47 Gene_expression denotes transfected
T25811 36-47 Gene_expression denotes transfected
T25812 53-58 Protein denotes RUNX1
T25813 62-67 Protein denotes RUNX3
T25814 1103-1108 Protein denotes RUNX1
T25815 1112-1117 Protein denotes RUNX3
R20125 T25812 T25811 themeOf RUNX1,transfected
R20126 T25813 T25810 themeOf RUNX3,transfected

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T25796 53-58 Protein denotes RUNX1
T25797 62-67 Protein denotes RUNX3
T25798 36-47 Gene_expression denotes transfected
T25799 36-47 Gene_expression denotes transfected
T25800 1103-1108 Protein denotes RUNX1
T25801 1112-1128 Protein denotes RUNX3 antibodies
T25802 1097-1102 Gene_expression denotes using
T25803 1097-1102 Gene_expression denotes using
R20119 T25796 T25798 themeOf RUNX1,transfected
R20120 T25797 T25799 themeOf RUNX3,transfected
R20121 T25800 T25802 themeOf RUNX1,using
R20122 T25801 T25803 themeOf RUNX3 antibodies,using

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T25358 36-47 Gene_expression denotes transfected
T25359 36-47 Gene_expression denotes transfected
T25360 53-58 Protein denotes RUNX1
T25361 62-67 Protein denotes RUNX3
T25362 1103-1108 Protein denotes RUNX1
T25363 1112-1117 Protein denotes RUNX3
R19714 T25360 T25359 themeOf RUNX1,transfected
R19715 T25361 T25358 themeOf RUNX3,transfected

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T25564 53-58 Q01196 denotes RUNX1
T25565 62-67 Q13761 denotes RUNX3
T25566 223-225 P0A7Z4 denotes pH
T25567 538-542 Q01196 denotes RUNX
T25568 538-542 Q13950 denotes RUNX
T25569 538-542 Q13761 denotes RUNX
T25570 1103-1108 Q01196 denotes RUNX1
T25571 1112-1117 Q13761 denotes RUNX3

test2

Id Subject Object Predicate Lexical cue
T25353 36-47 Gene_expression denotes transfected
T25354 53-58 Protein denotes RUNX1
T25355 62-67 Protein denotes RUNX3
T25356 1103-1108 Protein denotes RUNX1
T25357 1112-1117 Protein denotes RUNX3
R19712 T25354 T25353 themeOf RUNX1,transfected
R19713 T25355 T25353 themeOf RUNX3,transfected