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{"project":"UBERON-AE","denotations":[{"id":"T23280","span":{"begin":643,"end":648},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"GO-BP","denotations":[{"id":"T23619","span":{"begin":0,"end":16},"obj":"http://purl.obolibrary.org/obo/GO_0016246"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"GO-MF","denotations":[{"id":"T23620","span":{"begin":697,"end":701},"obj":"http://purl.obolibrary.org/obo/GO_0005134"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"GO-CC","denotations":[{"id":"T23621","span":{"begin":39,"end":44},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"sentences","denotations":[{"id":"T23292","span":{"begin":0,"end":17},"obj":"Sentence"},{"id":"T23293","span":{"begin":18,"end":195},"obj":"Sentence"},{"id":"T23294","span":{"begin":196,"end":427},"obj":"Sentence"},{"id":"T23295","span":{"begin":428,"end":515},"obj":"Sentence"},{"id":"T23296","span":{"begin":516,"end":619},"obj":"Sentence"},{"id":"T23297","span":{"begin":620,"end":710},"obj":"Sentence"},{"id":"T23298","span":{"begin":711,"end":819},"obj":"Sentence"},{"id":"T322","span":{"begin":0,"end":17},"obj":"Sentence"},{"id":"T323","span":{"begin":18,"end":195},"obj":"Sentence"},{"id":"T324","span":{"begin":196,"end":427},"obj":"Sentence"},{"id":"T325","span":{"begin":428,"end":515},"obj":"Sentence"},{"id":"T326","span":{"begin":516,"end":619},"obj":"Sentence"},{"id":"T327","span":{"begin":620,"end":710},"obj":"Sentence"},{"id":"T328","span":{"begin":711,"end":819},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"events-check-again","denotations":[{"id":"T23650","span":{"begin":590,"end":593},"obj":"Protein"},{"id":"T23651","span":{"begin":600,"end":603},"obj":"Protein"},{"id":"T23652","span":{"begin":614,"end":618},"obj":"Protein"},{"id":"T23653","span":{"begin":654,"end":659},"obj":"Protein"},{"id":"T23654","span":{"begin":697,"end":701},"obj":"Protein"},{"id":"T23644","span":{"begin":18,"end":21},"obj":"Protein"},{"id":"T23645","span":{"begin":32,"end":35},"obj":"Protein"},{"id":"T23646","span":{"begin":252,"end":258},"obj":"Negative_regulation"},{"id":"T23647","span":{"begin":263,"end":268},"obj":"Protein"},{"id":"T23648","span":{"begin":322,"end":328},"obj":"Negative_regulation"},{"id":"T23649","span":{"begin":333,"end":338},"obj":"Protein"}],"relations":[{"id":"R18308","pred":"themeOf","subj":"T23647","obj":"T23646"},{"id":"R18309","pred":"themeOf","subj":"T23649","obj":"T23648"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T23622","span":{"begin":18,"end":22},"obj":"Protein"},{"id":"T23623","span":{"begin":32,"end":36},"obj":"Protein"},{"id":"T23624","span":{"begin":129,"end":139},"obj":"Protein"},{"id":"T23625","span":{"begin":263,"end":268},"obj":"Protein"},{"id":"T23626","span":{"begin":333,"end":338},"obj":"Protein"},{"id":"T23627","span":{"begin":252,"end":258},"obj":"Negative_regulation"},{"id":"T23628","span":{"begin":322,"end":328},"obj":"Negative_regulation"},{"id":"T23629","span":{"begin":322,"end":328},"obj":"Negative_regulation"},{"id":"T23630","span":{"begin":590,"end":593},"obj":"Protein"},{"id":"T23631","span":{"begin":600,"end":603},"obj":"Protein"},{"id":"T23632","span":{"begin":609,"end":618},"obj":"Protein"}],"relations":[{"id":"R18303","pred":"themeOf","subj":"T23625","obj":"T23627"},{"id":"R18304","pred":"themeOf","subj":"T23625","obj":"T23628"},{"id":"R18305","pred":"themeOf","subj":"T23626","obj":"T23629"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T23281","span":{"begin":18,"end":21},"obj":"Protein"},{"id":"T23282","span":{"begin":32,"end":35},"obj":"Protein"},{"id":"T23283","span":{"begin":252,"end":258},"obj":"Negative_regulation"},{"id":"T23284","span":{"begin":263,"end":268},"obj":"Protein"},{"id":"T23285","span":{"begin":322,"end":328},"obj":"Negative_regulation"},{"id":"T23286","span":{"begin":333,"end":338},"obj":"Protein"},{"id":"T23287","span":{"begin":590,"end":593},"obj":"Protein"},{"id":"T23288","span":{"begin":600,"end":603},"obj":"Protein"},{"id":"T23289","span":{"begin":614,"end":618},"obj":"Protein"},{"id":"T23290","span":{"begin":654,"end":659},"obj":"Protein"},{"id":"T23291","span":{"begin":697,"end":701},"obj":"Protein"}],"relations":[{"id":"R18004","pred":"themeOf","subj":"T23284","obj":"T23283"},{"id":"R18005","pred":"themeOf","subj":"T23286","obj":"T23285"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T23440","span":{"begin":18,"end":21},"obj":"P01730"},{"id":"T23441","span":{"begin":32,"end":35},"obj":"P01730"},{"id":"T23442","span":{"begin":263,"end":268},"obj":"Q01196"},{"id":"T23443","span":{"begin":333,"end":338},"obj":"Q13761"},{"id":"T23444","span":{"begin":590,"end":593},"obj":"P06729"},{"id":"T23445","span":{"begin":600,"end":603},"obj":"P20963"},{"id":"T23446","span":{"begin":600,"end":603},"obj":"P07766"},{"id":"T23447","span":{"begin":600,"end":603},"obj":"P09693"},{"id":"T23448","span":{"begin":600,"end":603},"obj":"P04234"},{"id":"T23449","span":{"begin":614,"end":618},"obj":"P10747"},{"id":"T23450","span":{"begin":654,"end":657},"obj":"P26358"},{"id":"T23451","span":{"begin":697,"end":701},"obj":"P60568"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}

{"project":"test2","denotations":[{"id":"T23269","span":{"begin":18,"end":21},"obj":"Protein"},{"id":"T23270","span":{"begin":32,"end":35},"obj":"Protein"},{"id":"T23271","span":{"begin":252,"end":258},"obj":"Negative_regulation"},{"id":"T23272","span":{"begin":263,"end":268},"obj":"Protein"},{"id":"T23273","span":{"begin":322,"end":328},"obj":"Negative_regulation"},{"id":"T23274","span":{"begin":333,"end":338},"obj":"Protein"},{"id":"T23275","span":{"begin":590,"end":593},"obj":"Protein"},{"id":"T23276","span":{"begin":600,"end":603},"obj":"Protein"},{"id":"T23277","span":{"begin":614,"end":618},"obj":"Protein"},{"id":"T23278","span":{"begin":654,"end":659},"obj":"Protein"},{"id":"T23279","span":{"begin":697,"end":701},"obj":"Protein"}],"relations":[{"id":"R18002","pred":"themeOf","subj":"T23272","obj":"T23271"},{"id":"R18003","pred":"themeOf","subj":"T23274","obj":"T23273"}],"text":"RNA interference.\nCD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h."}