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PMC:2806624 / 47131-47950
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23299 | 0-3 | NN | denotes | RNA |
T23300 | 4-16 | NN | denotes | interference |
T23301 | 18-22 | JJ | denotes | CD4+ |
T23302 | 23-25 | CC | denotes | or |
T23303 | 26-31 | JJ | denotes | naive |
T23304 | 32-36 | JJ | denotes | CD4+ |
T23305 | 37-38 | NN | denotes | T |
T23306 | 39-44 | NN | denotes | cells |
T23307 | 45-49 | VB | denotes | were |
T23308 | 50-61 | VB | denotes | resuspended |
T23309 | 62-64 | IN | denotes | in |
T23310 | 65-68 | CD | denotes | 100 |
T23311 | 69-71 | NN | denotes | µl |
T23312 | 72-74 | IN | denotes | of |
T23313 | 75-87 | NN | denotes | Nucleofector |
T23314 | 88-96 | NN | denotes | solution |
T23315 | 97-98 | -LRB- | denotes | ( |
T23316 | 98-103 | NN | denotes | Lonza |
T23317 | 103-104 | -RRB- | denotes | ) |
T23318 | 105-108 | CC | denotes | and |
T23319 | 109-123 | VB | denotes | electroporated |
T23320 | 124-128 | IN | denotes | with |
T23321 | 129-130 | CD | denotes | 2 |
T23322 | 131-133 | NN | denotes | µM |
T23323 | 134-139 | NN | denotes | siRNA |
T23324 | 140-145 | VB | denotes | using |
T23325 | 146-149 | DT | denotes | the |
T23326 | 150-162 | NN | denotes | Nucleofector |
T23327 | 163-173 | NN | denotes | technology |
T23328 | 174-181 | NN | denotes | program |
T23329 | 182-186 | NN | denotes | U-14 |
T23330 | 187-188 | -LRB- | denotes | ( |
T23331 | 188-193 | NN | denotes | Lonza |
T23332 | 193-194 | -RRB- | denotes | ) |
T23333 | 196-200 | CD | denotes | Five |
T23334 | 201-210 | JJ | denotes | different |
T23335 | 211-219 | NN | denotes | Silencer |
T23336 | 220-222 | CC | denotes | or |
T23337 | 223-231 | NNP | denotes | Silencer |
T23338 | 232-238 | NNP | denotes | Select |
T23339 | 239-251 | JJ | denotes | Pre-designed |
T23340 | 252-258 | NN | denotes | siRNAs |
T23341 | 259-262 | IN | denotes | for |
T23342 | 263-268 | NN | denotes | RUNX1 |
T23343 | 269-270 | -LRB- | denotes | ( |
T23344 | 270-277 | NNP | denotes | Applied |
T23345 | 278-288 | NNP | denotes | Biosystems |
T23346 | 288-289 | -RRB- | denotes | ) |
T23347 | 290-293 | CC | denotes | and |
T23348 | 294-299 | CD | denotes | three |
T23349 | 300-308 | NN | denotes | Silencer |
T23350 | 309-321 | JJ | denotes | Pre-designed |
T23351 | 322-328 | NN | denotes | siRNAs |
T23352 | 329-332 | IN | denotes | for |
T23353 | 333-338 | NN | denotes | RUNX3 |
T23354 | 339-340 | -LRB- | denotes | ( |
T23355 | 340-347 | NNP | denotes | Applied |
T23356 | 348-358 | NNP | denotes | Biosystems |
T23357 | 358-359 | -RRB- | denotes | ) |
T23358 | 360-364 | VB | denotes | were |
T23359 | 365-371 | VB | denotes | tested |
T23360 | 371-372 | -COMMA- | denotes | , |
T23361 | 373-376 | CC | denotes | and |
T23362 | 377-380 | DT | denotes | the |
T23363 | 381-385 | JJ | denotes | best |
T23364 | 386-389 | VB | denotes | was |
T23365 | 390-398 | VB | denotes | selected |
T23366 | 399-402 | IN | denotes | for |
T23367 | 403-406 | DT | denotes | all |
T23368 | 407-414 | JJ | denotes | further |
T23369 | 415-426 | NN | denotes | experiments |
T23370 | 428-431 | DT | denotes | The |
T23371 | 432-440 | NNP | denotes | Silencer |
T23372 | 441-449 | JJ | denotes | Negative |
T23373 | 450-457 | NNP | denotes | Control |
T23374 | 458-459 | -SHARP- | denotes | # |
T23375 | 459-460 | CD | denotes | 1 |
T23376 | 461-466 | NN | denotes | siRNA |
T23377 | 467-468 | -LRB- | denotes | ( |
T23378 | 468-475 | NNP | denotes | Applied |
T23379 | 476-486 | NNP | denotes | Biosystems |
T23380 | 486-487 | -RRB- | denotes | ) |
T23381 | 488-491 | VB | denotes | was |
T23382 | 492-496 | VB | denotes | used |
T23383 | 497-500 | IN | denotes | for |
T23384 | 501-514 | NN | denotes | normalization |
T23385 | 516-521 | NN | denotes | Cells |
T23386 | 522-526 | VB | denotes | were |
T23387 | 527-531 | RB | denotes | then |
T23388 | 532-536 | VB | denotes | left |
T23389 | 537-549 | JJ | denotes | unstimulated |
T23390 | 550-552 | CC | denotes | or |
T23391 | 553-557 | VB | denotes | were |
T23392 | 558-568 | VB | denotes | stimulated |
T23393 | 569-574 | IN | denotes | after |
T23394 | 575-577 | CD | denotes | 12 |
T23395 | 578-579 | NN | denotes | h |
T23396 | 580-584 | IN | denotes | with |
T23397 | 585-593 | NN | denotes | anti-CD2 |
T23398 | 593-594 | -COMMA- | denotes | , |
T23399 | 595-603 | NN | denotes | anti-CD3 |
T23400 | 603-604 | -COMMA- | denotes | , |
T23401 | 605-608 | CC | denotes | and |
T23402 | 609-618 | NN | denotes | anti-CD28 |
T23403 | 620-625 | NN | denotes | Cells |
T23404 | 626-630 | VB | denotes | were |
T23405 | 631-639 | VB | denotes | cultured |
T23406 | 640-642 | IN | denotes | in |
T23407 | 643-653 | JJ | denotes | serum-free |
T23408 | 654-659 | NN | denotes | AIM-V |
T23409 | 660-666 | NN | denotes | medium |
T23410 | 667-671 | IN | denotes | with |
T23411 | 672-675 | DT | denotes | the |
T23412 | 676-684 | NN | denotes | addition |
T23413 | 685-687 | IN | denotes | of |
T23414 | 688-689 | CD | denotes | 1 |
T23415 | 690-696 | NN | denotes | nmol/l |
T23416 | 697-701 | NN | denotes | IL-2 |
T23417 | 702-703 | -LRB- | denotes | ( |
T23418 | 703-708 | NN | denotes | Roche |
T23419 | 708-709 | -RRB- | denotes | ) |
T23420 | 711-716 | NN | denotes | Cells |
T23421 | 717-721 | VB | denotes | were |
T23422 | 722-731 | VB | denotes | harvested |
T23423 | 732-735 | IN | denotes | for |
T23424 | 736-740 | NN | denotes | mRNA |
T23425 | 741-750 | NN | denotes | detection |
T23426 | 751-753 | IN | denotes | of |
T23427 | 754-757 | DT | denotes | the |
T23428 | 758-764 | NN | denotes | target |
T23429 | 765-770 | NN | denotes | genes |
T23430 | 771-776 | IN | denotes | after |
T23431 | 777-779 | CD | denotes | 24 |
T23432 | 780-781 | NN | denotes | h |
T23433 | 782-785 | CC | denotes | and |
T23434 | 786-789 | IN | denotes | for |
T23435 | 790-797 | NN | denotes | protein |
T23436 | 798-807 | NN | denotes | detection |
T23437 | 808-813 | IN | denotes | after |
T23438 | 814-816 | CD | denotes | 48 |
T23439 | 817-818 | NN | denotes | h |
R18006 | T23300 | T23299 | arg1Of | interference,RNA |
R18007 | T23301 | T23302 | arg1Of | CD4+,or |
R18008 | T23303 | T23302 | arg2Of | naive,or |
R18009 | T23306 | T23301 | arg1Of | cells,CD4+ |
R18010 | T23306 | T23303 | arg1Of | cells,naive |
R18011 | T23306 | T23304 | arg1Of | cells,CD4+ |
R18012 | T23306 | T23305 | arg1Of | cells,T |
R18013 | T23306 | T23307 | arg1Of | cells,were |
R18014 | T23306 | T23308 | arg2Of | cells,resuspended |
R18015 | T23306 | T23319 | arg2Of | cells,electroporated |
R18016 | T23308 | T23309 | arg1Of | resuspended,in |
R18017 | T23308 | T23318 | arg1Of | resuspended,and |
R18018 | T23311 | T23309 | arg2Of | µl,in |
R18019 | T23311 | T23310 | arg1Of | µl,100 |
R18020 | T23311 | T23312 | arg1Of | µl,of |
R18021 | T23314 | T23312 | arg2Of | solution,of |
R18022 | T23314 | T23313 | arg1Of | solution,Nucleofector |
R18023 | T23314 | T23315 | arg1Of | solution,( |
R18024 | T23316 | T23315 | arg2Of | Lonza,( |
R18025 | T23317 | T23315 | arg3Of | ),( |
R18026 | T23318 | T23307 | arg2Of | and,were |
R18027 | T23319 | T23318 | arg2Of | electroporated,and |
R18028 | T23319 | T23320 | arg1Of | electroporated,with |
R18029 | T23323 | T23320 | arg2Of | siRNA,with |
R18030 | T23323 | T23321 | arg1Of | siRNA,2 |
R18031 | T23323 | T23322 | arg1Of | siRNA,µM |
R18032 | T23323 | T23324 | arg1Of | siRNA,using |
R18033 | T23329 | T23324 | arg2Of | U-14,using |
R18034 | T23329 | T23325 | arg1Of | U-14,the |
R18035 | T23329 | T23326 | arg1Of | U-14,Nucleofector |
R18036 | T23329 | T23327 | arg1Of | U-14,technology |
R18037 | T23329 | T23328 | arg1Of | U-14,program |
R18038 | T23329 | T23330 | arg1Of | U-14,( |
R18039 | T23331 | T23330 | arg2Of | Lonza,( |
R18040 | T23332 | T23330 | arg3Of | ),( |
R18041 | T23335 | T23333 | arg1Of | Silencer,Five |
R18042 | T23335 | T23334 | arg1Of | Silencer,different |
R18043 | T23335 | T23336 | arg1Of | Silencer,or |
R18044 | T23336 | T23341 | arg1Of | or,for |
R18045 | T23336 | T23358 | arg1Of | or,were |
R18046 | T23336 | T23359 | arg2Of | or,tested |
R18047 | T23338 | T23337 | arg1Of | Select,Silencer |
R18048 | T23340 | T23336 | arg2Of | siRNAs,or |
R18049 | T23340 | T23338 | arg1Of | siRNAs,Select |
R18050 | T23340 | T23339 | arg1Of | siRNAs,Pre-designed |
R18051 | T23342 | T23343 | arg1Of | RUNX1,( |
R18052 | T23342 | T23347 | arg1Of | RUNX1,and |
R18053 | T23345 | T23343 | arg2Of | Biosystems,( |
R18054 | T23345 | T23344 | arg1Of | Biosystems,Applied |
R18055 | T23346 | T23343 | arg3Of | ),( |
R18056 | T23347 | T23341 | arg2Of | and,for |
R18057 | T23351 | T23347 | arg2Of | siRNAs,and |
R18058 | T23351 | T23348 | arg1Of | siRNAs,three |
R18059 | T23351 | T23349 | arg1Of | siRNAs,Silencer |
R18060 | T23351 | T23350 | arg1Of | siRNAs,Pre-designed |
R18061 | T23351 | T23352 | arg1Of | siRNAs,for |
R18062 | T23353 | T23352 | arg2Of | RUNX3,for |
R18063 | T23353 | T23354 | arg1Of | RUNX3,( |
R18064 | T23356 | T23354 | arg2Of | Biosystems,( |
R18065 | T23356 | T23355 | arg1Of | Biosystems,Applied |
R18066 | T23357 | T23354 | arg3Of | ),( |
R18067 | T23359 | T23358 | arg2Of | tested,were |
R18068 | T23359 | T23361 | arg1Of | tested,and |
R18069 | T23361 | T23360 | arg1Of | and,"," |
R18070 | T23363 | T23362 | arg1Of | best,the |
R18071 | T23363 | T23364 | arg1Of | best,was |
R18072 | T23363 | T23365 | arg2Of | best,selected |
R18073 | T23365 | T23361 | arg2Of | selected,and |
R18074 | T23365 | T23364 | arg2Of | selected,was |
R18075 | T23365 | T23366 | arg1Of | selected,for |
R18076 | T23369 | T23366 | arg2Of | experiments,for |
R18077 | T23369 | T23367 | arg1Of | experiments,all |
R18078 | T23369 | T23368 | arg1Of | experiments,further |
R18079 | T23372 | T23371 | arg1Of | Negative,Silencer |
R18080 | T23376 | T23370 | arg1Of | siRNA,The |
R18081 | T23376 | T23372 | arg1Of | siRNA,Negative |
R18082 | T23376 | T23373 | arg1Of | siRNA,Control |
R18083 | T23376 | T23374 | arg1Of | siRNA,# |
R18084 | T23376 | T23375 | arg1Of | siRNA,1 |
R18085 | T23376 | T23377 | arg1Of | siRNA,( |
R18086 | T23376 | T23381 | arg1Of | siRNA,was |
R18087 | T23376 | T23382 | arg2Of | siRNA,used |
R18088 | T23379 | T23377 | arg2Of | Biosystems,( |
R18089 | T23379 | T23378 | arg1Of | Biosystems,Applied |
R18090 | T23380 | T23377 | arg3Of | ),( |
R18091 | T23382 | T23381 | arg2Of | used,was |
R18092 | T23382 | T23383 | arg1Of | used,for |
R18093 | T23384 | T23383 | arg2Of | normalization,for |
R18094 | T23385 | T23386 | arg1Of | Cells,were |
R18095 | T23385 | T23388 | arg2Of | Cells,left |
R18096 | T23385 | T23391 | arg1Of | Cells,were |
R18097 | T23385 | T23392 | arg2Of | Cells,stimulated |
R18098 | T23388 | T23386 | arg2Of | left,were |
R18099 | T23388 | T23387 | arg1Of | left,then |
R18100 | T23388 | T23390 | arg1Of | left,or |
R18101 | T23389 | T23388 | arg3Of | unstimulated,left |
R18102 | T23392 | T23390 | arg2Of | stimulated,or |
R18103 | T23392 | T23391 | arg2Of | stimulated,were |
R18104 | T23392 | T23393 | arg1Of | stimulated,after |
R18105 | T23395 | T23393 | arg2Of | h,after |
R18106 | T23395 | T23394 | arg1Of | h,12 |
R18107 | T23395 | T23396 | arg1Of | h,with |
R18108 | T23397 | T23398 | arg1Of | anti-CD2,"," |
R18109 | T23398 | T23401 | arg1Of | ",",and |
R18110 | T23399 | T23398 | arg2Of | anti-CD3,"," |
R18111 | T23401 | T23396 | arg2Of | and,with |
R18112 | T23401 | T23400 | arg1Of | and,"," |
R18113 | T23402 | T23401 | arg2Of | anti-CD28,and |
R18114 | T23403 | T23404 | arg1Of | Cells,were |
R18115 | T23403 | T23405 | arg2Of | Cells,cultured |
R18116 | T23405 | T23404 | arg2Of | cultured,were |
R18117 | T23405 | T23406 | arg1Of | cultured,in |
R18118 | T23409 | T23406 | arg2Of | medium,in |
R18119 | T23409 | T23407 | arg1Of | medium,serum-free |
R18120 | T23409 | T23408 | arg1Of | medium,AIM-V |
R18121 | T23409 | T23410 | arg1Of | medium,with |
R18122 | T23412 | T23410 | arg2Of | addition,with |
R18123 | T23412 | T23411 | arg1Of | addition,the |
R18124 | T23412 | T23413 | arg1Of | addition,of |
R18125 | T23416 | T23413 | arg2Of | IL-2,of |
R18126 | T23416 | T23414 | arg1Of | IL-2,1 |
R18127 | T23416 | T23415 | arg1Of | IL-2,nmol/l |
R18128 | T23416 | T23417 | arg1Of | IL-2,( |
R18129 | T23418 | T23417 | arg2Of | Roche,( |
R18130 | T23419 | T23417 | arg3Of | ),( |
R18131 | T23420 | T23421 | arg1Of | Cells,were |
R18132 | T23420 | T23422 | arg2Of | Cells,harvested |
R18133 | T23422 | T23421 | arg2Of | harvested,were |
R18134 | T23422 | T23423 | arg1Of | harvested,for |
R18135 | T23422 | T23430 | arg1Of | harvested,after |
R18136 | T23422 | T23434 | arg1Of | harvested,for |
R18137 | T23422 | T23437 | arg1Of | harvested,after |
R18138 | T23425 | T23423 | arg2Of | detection,for |
R18139 | T23425 | T23424 | arg1Of | detection,mRNA |
R18140 | T23425 | T23426 | arg1Of | detection,of |
R18141 | T23429 | T23426 | arg2Of | genes,of |
R18142 | T23429 | T23427 | arg1Of | genes,the |
R18143 | T23429 | T23428 | arg1Of | genes,target |
R18144 | T23430 | T23433 | arg1Of | after,and |
R18145 | T23432 | T23430 | arg2Of | h,after |
R18146 | T23432 | T23431 | arg1Of | h,24 |
R18147 | T23434 | T23433 | arg2Of | for,and |
R18148 | T23436 | T23434 | arg2Of | detection,for |
R18149 | T23436 | T23435 | arg1Of | detection,protein |
R18150 | T23439 | T23437 | arg2Of | h,after |
R18151 | T23439 | T23438 | arg1Of | h,48 |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23459 | 0-3 | NNP | denotes | RNA |
T23460 | 4-16 | NN | denotes | interference |
T23461 | 16-17 | . | denotes | . |
T23462 | 18-21 | CD | denotes | CD4 |
T23463 | 21-22 | NN | denotes | + |
T23464 | 23-25 | CC | denotes | or |
T23465 | 26-31 | JJ | denotes | naive |
T23466 | 32-35 | NNP | denotes | CD4 |
T23467 | 35-36 | CD | denotes | + |
T23468 | 37-38 | NNP | denotes | T |
T23469 | 39-44 | NNS | denotes | cells |
T23470 | 45-49 | VBD | denotes | were |
T23471 | 50-61 | VBN | denotes | resuspended |
T23472 | 62-64 | IN | denotes | in |
T23473 | 65-68 | CD | denotes | 100 |
T23474 | 69-71 | NN | denotes | µl |
T23475 | 72-74 | IN | denotes | of |
T23476 | 75-87 | NNP | denotes | Nucleofector |
T23477 | 88-96 | NN | denotes | solution |
T23478 | 97-98 | -LRB- | denotes | ( |
T23479 | 98-103 | NNP | denotes | Lonza |
T23480 | 103-104 | -RRB- | denotes | ) |
T23481 | 105-108 | CC | denotes | and |
T23482 | 109-123 | VBD | denotes | electroporated |
T23483 | 124-128 | IN | denotes | with |
T23484 | 129-130 | CD | denotes | 2 |
T23485 | 131-133 | NNP | denotes | µM |
T23486 | 134-139 | NNP | denotes | siRNA |
T23487 | 140-145 | VBG | denotes | using |
T23488 | 146-149 | DT | denotes | the |
T23489 | 150-162 | NNP | denotes | Nucleofector |
T23490 | 163-173 | NN | denotes | technology |
T23491 | 174-181 | NN | denotes | program |
T23492 | 182-186 | NN | denotes | U-14 |
T23493 | 187-188 | -LRB- | denotes | ( |
T23494 | 188-193 | NNP | denotes | Lonza |
T23495 | 193-194 | -RRB- | denotes | ) |
T23496 | 194-195 | . | denotes | . |
T23497 | 196-200 | CD | denotes | Five |
T23498 | 201-210 | JJ | denotes | different |
T23499 | 211-219 | NN | denotes | Silencer |
T23500 | 220-222 | CC | denotes | or |
T23501 | 223-231 | NNP | denotes | Silencer |
T23502 | 232-238 | NNP | denotes | Select |
T23503 | 239-251 | JJ | denotes | Pre-designed |
T23504 | 252-258 | NNS | denotes | siRNAs |
T23505 | 259-262 | IN | denotes | for |
T23506 | 263-268 | NNP | denotes | RUNX1 |
T23507 | 269-270 | -LRB- | denotes | ( |
T23508 | 270-277 | NNP | denotes | Applied |
T23509 | 278-288 | NNPS | denotes | Biosystems |
T23510 | 288-289 | -RRB- | denotes | ) |
T23511 | 290-293 | CC | denotes | and |
T23512 | 294-299 | CD | denotes | three |
T23513 | 300-308 | NNP | denotes | Silencer |
T23514 | 309-321 | JJ | denotes | Pre-designed |
T23515 | 322-328 | NNS | denotes | siRNAs |
T23516 | 329-332 | IN | denotes | for |
T23517 | 333-338 | NNP | denotes | RUNX3 |
T23518 | 339-340 | -LRB- | denotes | ( |
T23519 | 340-347 | NNP | denotes | Applied |
T23520 | 348-358 | NNPS | denotes | Biosystems |
T23521 | 358-359 | -RRB- | denotes | ) |
T23522 | 360-364 | VBD | denotes | were |
T23523 | 365-371 | VBN | denotes | tested |
T23524 | 371-372 | , | denotes | , |
T23525 | 373-376 | CC | denotes | and |
T23526 | 377-380 | DT | denotes | the |
T23527 | 381-385 | JJS | denotes | best |
T23528 | 386-389 | VBD | denotes | was |
T23529 | 390-398 | VBN | denotes | selected |
T23530 | 399-402 | IN | denotes | for |
T23531 | 403-406 | DT | denotes | all |
T23532 | 407-414 | JJ | denotes | further |
T23533 | 415-426 | NNS | denotes | experiments |
T23534 | 426-427 | . | denotes | . |
T23535 | 428-431 | DT | denotes | The |
T23536 | 432-440 | NNP | denotes | Silencer |
T23537 | 441-449 | JJ | denotes | Negative |
T23538 | 450-457 | NNP | denotes | Control |
T23539 | 458-459 | # | denotes | # |
T23540 | 459-460 | CD | denotes | 1 |
T23541 | 461-466 | NNP | denotes | siRNA |
T23542 | 467-468 | -LRB- | denotes | ( |
T23543 | 468-475 | NNP | denotes | Applied |
T23544 | 476-486 | NNPS | denotes | Biosystems |
T23545 | 486-487 | -RRB- | denotes | ) |
T23546 | 488-491 | VBD | denotes | was |
T23547 | 492-496 | VBN | denotes | used |
T23548 | 497-500 | IN | denotes | for |
T23549 | 501-514 | NN | denotes | normalization |
T23550 | 514-515 | . | denotes | . |
T23551 | 516-521 | NNS | denotes | Cells |
T23552 | 522-526 | VBD | denotes | were |
T23553 | 527-531 | RB | denotes | then |
T23554 | 532-536 | VBN | denotes | left |
T23555 | 537-549 | JJ | denotes | unstimulated |
T23556 | 550-552 | CC | denotes | or |
T23557 | 553-557 | VBD | denotes | were |
T23558 | 558-568 | VBN | denotes | stimulated |
T23559 | 569-574 | IN | denotes | after |
T23560 | 575-577 | CD | denotes | 12 |
T23561 | 578-579 | NN | denotes | h |
T23562 | 580-584 | IN | denotes | with |
T23563 | 585-593 | JJ | denotes | anti-CD2 |
T23564 | 593-594 | , | denotes | , |
T23565 | 595-603 | JJ | denotes | anti-CD3 |
T23566 | 603-604 | , | denotes | , |
T23567 | 605-608 | CC | denotes | and |
T23568 | 609-618 | JJ | denotes | anti-CD28 |
T23569 | 618-619 | . | denotes | . |
T23570 | 620-625 | NNS | denotes | Cells |
T23571 | 626-630 | VBD | denotes | were |
T23572 | 631-639 | VBN | denotes | cultured |
T23573 | 640-642 | IN | denotes | in |
T23574 | 643-653 | JJ | denotes | serum-free |
T23575 | 654-659 | NNP | denotes | AIM-V |
T23576 | 660-666 | NN | denotes | medium |
T23577 | 667-671 | IN | denotes | with |
T23578 | 672-675 | DT | denotes | the |
T23579 | 676-684 | NN | denotes | addition |
T23580 | 685-687 | IN | denotes | of |
T23581 | 688-689 | CD | denotes | 1 |
T23582 | 690-696 | NN | denotes | nmol/l |
T23583 | 697-701 | NN | denotes | IL-2 |
T23584 | 702-703 | -LRB- | denotes | ( |
T23585 | 703-708 | NNP | denotes | Roche |
T23586 | 708-709 | -RRB- | denotes | ) |
T23587 | 709-710 | . | denotes | . |
T23588 | 711-716 | NNS | denotes | Cells |
T23589 | 717-721 | VBD | denotes | were |
T23590 | 722-731 | VBN | denotes | harvested |
T23591 | 732-735 | IN | denotes | for |
T23592 | 736-740 | NNP | denotes | mRNA |
T23593 | 741-750 | NN | denotes | detection |
T23594 | 751-753 | IN | denotes | of |
T23595 | 754-757 | DT | denotes | the |
T23596 | 758-764 | NN | denotes | target |
T23597 | 765-770 | NNS | denotes | genes |
T23598 | 771-776 | IN | denotes | after |
T23599 | 777-779 | CD | denotes | 24 |
T23600 | 780-781 | NN | denotes | h |
T23601 | 782-785 | CC | denotes | and |
T23602 | 786-789 | IN | denotes | for |
T23603 | 790-797 | NN | denotes | protein |
T23604 | 798-807 | NN | denotes | detection |
T23605 | 808-813 | IN | denotes | after |
T23606 | 814-816 | CD | denotes | 48 |
T23607 | 817-819 | NN | denotes | h. |
R18152 | T23459 | T23460 | compound | RNA,interference |
R18153 | T23460 | T23460 | ROOT | interference,interference |
R18154 | T23461 | T23460 | punct | .,interference |
R18155 | T23462 | T23469 | nummod | CD4,cells |
R18156 | T23463 | T23469 | nmod | +,cells |
R18157 | T23464 | T23463 | cc | or,+ |
R18158 | T23465 | T23463 | conj | naive,+ |
R18159 | T23466 | T23469 | compound | CD4,cells |
R18160 | T23467 | T23469 | nummod | +,cells |
R18161 | T23468 | T23469 | compound | T,cells |
R18162 | T23469 | T23471 | nsubjpass | cells,resuspended |
R18163 | T23470 | T23471 | auxpass | were,resuspended |
R18164 | T23471 | T23471 | ROOT | resuspended,resuspended |
R18165 | T23472 | T23471 | prep | in,resuspended |
R18166 | T23473 | T23474 | nummod | 100,µl |
R18167 | T23474 | T23472 | pobj | µl,in |
R18168 | T23475 | T23474 | prep | of,µl |
R18169 | T23476 | T23477 | compound | Nucleofector,solution |
R18170 | T23477 | T23475 | pobj | solution,of |
R18171 | T23478 | T23479 | punct | (,Lonza |
R18172 | T23479 | T23477 | appos | Lonza,solution |
R18173 | T23480 | T23479 | punct | ),Lonza |
R18174 | T23481 | T23471 | cc | and,resuspended |
R18175 | T23482 | T23471 | conj | electroporated,resuspended |
R18176 | T23483 | T23482 | prep | with,electroporated |
R18177 | T23484 | T23486 | nummod | 2,siRNA |
R18178 | T23485 | T23486 | compound | µM,siRNA |
R18179 | T23486 | T23487 | nsubj | siRNA,using |
R18180 | T23487 | T23483 | pcomp | using,with |
R18181 | T23488 | T23491 | det | the,program |
R18182 | T23489 | T23491 | nmod | Nucleofector,program |
R18183 | T23490 | T23491 | compound | technology,program |
R18184 | T23491 | T23492 | compound | program,U-14 |
R18185 | T23492 | T23487 | dobj | U-14,using |
R18186 | T23493 | T23492 | punct | (,U-14 |
R18187 | T23494 | T23492 | appos | Lonza,U-14 |
R18188 | T23495 | T23492 | punct | ),U-14 |
R18189 | T23496 | T23471 | punct | .,resuspended |
R18190 | T23497 | T23499 | nummod | Five,Silencer |
R18191 | T23498 | T23499 | amod | different,Silencer |
R18192 | T23499 | T23523 | nsubjpass | Silencer,tested |
R18193 | T23500 | T23499 | cc | or,Silencer |
R18194 | T23501 | T23499 | conj | Silencer,Silencer |
R18195 | T23502 | T23504 | compound | Select,siRNAs |
R18196 | T23503 | T23504 | compound | Pre-designed,siRNAs |
R18197 | T23504 | T23499 | appos | siRNAs,Silencer |
R18198 | T23505 | T23504 | prep | for,siRNAs |
R18199 | T23506 | T23505 | pobj | RUNX1,for |
R18200 | T23507 | T23509 | punct | (,Biosystems |
R18201 | T23508 | T23509 | compound | Applied,Biosystems |
R18202 | T23509 | T23523 | nsubjpass | Biosystems,tested |
R18203 | T23510 | T23509 | punct | ),Biosystems |
R18204 | T23511 | T23509 | cc | and,Biosystems |
R18205 | T23512 | T23515 | nummod | three,siRNAs |
R18206 | T23513 | T23515 | compound | Silencer,siRNAs |
R18207 | T23514 | T23515 | compound | Pre-designed,siRNAs |
R18208 | T23515 | T23509 | conj | siRNAs,Biosystems |
R18209 | T23516 | T23515 | prep | for,siRNAs |
R18210 | T23517 | T23516 | pobj | RUNX3,for |
R18211 | T23518 | T23520 | punct | (,Biosystems |
R18212 | T23519 | T23520 | compound | Applied,Biosystems |
R18213 | T23520 | T23517 | appos | Biosystems,RUNX3 |
R18214 | T23521 | T23517 | punct | ),RUNX3 |
R18215 | T23522 | T23523 | auxpass | were,tested |
R18216 | T23523 | T23523 | ROOT | tested,tested |
R18217 | T23524 | T23523 | punct | ",",tested |
R18218 | T23525 | T23523 | cc | and,tested |
R18219 | T23526 | T23527 | det | the,best |
R18220 | T23527 | T23529 | nsubjpass | best,selected |
R18221 | T23528 | T23529 | auxpass | was,selected |
R18222 | T23529 | T23523 | conj | selected,tested |
R18223 | T23530 | T23529 | prep | for,selected |
R18224 | T23531 | T23533 | det | all,experiments |
R18225 | T23532 | T23533 | amod | further,experiments |
R18226 | T23533 | T23530 | pobj | experiments,for |
R18227 | T23534 | T23529 | punct | .,selected |
R18228 | T23535 | T23538 | det | The,Control |
R18229 | T23536 | T23538 | compound | Silencer,Control |
R18230 | T23537 | T23538 | compound | Negative,Control |
R18231 | T23538 | T23547 | nsubjpass | Control,used |
R18232 | T23539 | T23547 | nsubjpass | #,used |
R18233 | T23540 | T23541 | nummod | 1,siRNA |
R18234 | T23541 | T23547 | nsubjpass | siRNA,used |
R18235 | T23542 | T23544 | punct | (,Biosystems |
R18236 | T23543 | T23544 | compound | Applied,Biosystems |
R18237 | T23544 | T23541 | parataxis | Biosystems,siRNA |
R18238 | T23545 | T23544 | punct | ),Biosystems |
R18239 | T23546 | T23547 | auxpass | was,used |
R18240 | T23547 | T23547 | ROOT | used,used |
R18241 | T23548 | T23547 | prep | for,used |
R18242 | T23549 | T23548 | pobj | normalization,for |
R18243 | T23550 | T23547 | punct | .,used |
R18244 | T23551 | T23554 | nsubjpass | Cells,left |
R18245 | T23552 | T23554 | auxpass | were,left |
R18246 | T23553 | T23554 | advmod | then,left |
R18247 | T23554 | T23554 | ROOT | left,left |
R18248 | T23555 | T23554 | oprd | unstimulated,left |
R18249 | T23556 | T23554 | cc | or,left |
R18250 | T23557 | T23558 | auxpass | were,stimulated |
R18251 | T23558 | T23554 | conj | stimulated,left |
R18252 | T23559 | T23558 | prep | after,stimulated |
R18253 | T23560 | T23561 | nummod | 12,h |
R18254 | T23561 | T23559 | pobj | h,after |
R18255 | T23562 | T23561 | prep | with,h |
R18256 | T23563 | T23562 | pobj | anti-CD2,with |
R18257 | T23564 | T23563 | punct | ",",anti-CD2 |
R18258 | T23565 | T23563 | conj | anti-CD3,anti-CD2 |
R18259 | T23566 | T23565 | punct | ",",anti-CD3 |
R18260 | T23567 | T23565 | cc | and,anti-CD3 |
R18261 | T23568 | T23565 | conj | anti-CD28,anti-CD3 |
R18262 | T23569 | T23554 | punct | .,left |
R18263 | T23570 | T23572 | nsubjpass | Cells,cultured |
R18264 | T23571 | T23572 | auxpass | were,cultured |
R18265 | T23572 | T23572 | ROOT | cultured,cultured |
R18266 | T23573 | T23572 | prep | in,cultured |
R18267 | T23574 | T23576 | amod | serum-free,medium |
R18268 | T23575 | T23576 | compound | AIM-V,medium |
R18269 | T23576 | T23573 | pobj | medium,in |
R18270 | T23577 | T23572 | prep | with,cultured |
R18271 | T23578 | T23579 | det | the,addition |
R18272 | T23579 | T23577 | pobj | addition,with |
R18273 | T23580 | T23579 | prep | of,addition |
R18274 | T23581 | T23582 | nummod | 1,nmol/l |
R18275 | T23582 | T23583 | compound | nmol/l,IL-2 |
R18276 | T23583 | T23580 | pobj | IL-2,of |
R18277 | T23584 | T23585 | punct | (,Roche |
R18278 | T23585 | T23583 | appos | Roche,IL-2 |
R18279 | T23586 | T23583 | punct | ),IL-2 |
R18280 | T23587 | T23572 | punct | .,cultured |
R18281 | T23588 | T23590 | nsubjpass | Cells,harvested |
R18282 | T23589 | T23590 | auxpass | were,harvested |
R18283 | T23590 | T23590 | ROOT | harvested,harvested |
R18284 | T23591 | T23590 | prep | for,harvested |
R18285 | T23592 | T23593 | compound | mRNA,detection |
R18286 | T23593 | T23591 | pobj | detection,for |
R18287 | T23594 | T23593 | prep | of,detection |
R18288 | T23595 | T23597 | det | the,genes |
R18289 | T23596 | T23597 | compound | target,genes |
R18290 | T23597 | T23594 | pobj | genes,of |
R18291 | T23598 | T23590 | prep | after,harvested |
R18292 | T23599 | T23600 | nummod | 24,h |
R18293 | T23600 | T23598 | pobj | h,after |
R18294 | T23601 | T23598 | cc | and,after |
R18295 | T23602 | T23598 | conj | for,after |
R18296 | T23603 | T23604 | compound | protein,detection |
R18297 | T23604 | T23602 | pobj | detection,for |
R18298 | T23605 | T23590 | prep | after,harvested |
R18299 | T23606 | T23607 | nummod | 48,h. |
R18300 | T23607 | T23605 | pobj | h.,after |
UBERON-AE
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23280 | 643-648 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23619 | 0-16 | http://purl.obolibrary.org/obo/GO_0016246 | denotes | RNA interference |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23620 | 697-701 | http://purl.obolibrary.org/obo/GO_0005134 | denotes | IL-2 |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23621 | 39-44 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23292 | 0-17 | Sentence | denotes | RNA interference. |
T23293 | 18-195 | Sentence | denotes | CD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). |
T23294 | 196-427 | Sentence | denotes | Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. |
T23295 | 428-515 | Sentence | denotes | The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. |
T23296 | 516-619 | Sentence | denotes | Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. |
T23297 | 620-710 | Sentence | denotes | Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). |
T23298 | 711-819 | Sentence | denotes | Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h. |
T322 | 0-17 | Sentence | denotes | RNA interference. |
T323 | 18-195 | Sentence | denotes | CD4+ or naive CD4+ T cells were resuspended in 100 µl of Nucleofector solution (Lonza) and electroporated with 2 µM siRNA using the Nucleofector technology program U-14 (Lonza). |
T324 | 196-427 | Sentence | denotes | Five different Silencer or Silencer Select Pre-designed siRNAs for RUNX1 (Applied Biosystems) and three Silencer Pre-designed siRNAs for RUNX3 (Applied Biosystems) were tested, and the best was selected for all further experiments. |
T325 | 428-515 | Sentence | denotes | The Silencer Negative Control #1 siRNA (Applied Biosystems) was used for normalization. |
T326 | 516-619 | Sentence | denotes | Cells were then left unstimulated or were stimulated after 12 h with anti-CD2, anti-CD3, and anti-CD28. |
T327 | 620-710 | Sentence | denotes | Cells were cultured in serum-free AIM-V medium with the addition of 1 nmol/l IL-2 (Roche). |
T328 | 711-819 | Sentence | denotes | Cells were harvested for mRNA detection of the target genes after 24 h and for protein detection after 48 h. |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23650 | 590-593 | Protein | denotes | CD2 |
T23651 | 600-603 | Protein | denotes | CD3 |
T23652 | 614-618 | Protein | denotes | CD28 |
T23653 | 654-659 | Protein | denotes | AIM-V |
T23654 | 697-701 | Protein | denotes | IL-2 |
T23644 | 18-21 | Protein | denotes | CD4 |
T23645 | 32-35 | Protein | denotes | CD4 |
T23646 | 252-258 | Negative_regulation | denotes | siRNAs |
T23647 | 263-268 | Protein | denotes | RUNX1 |
T23648 | 322-328 | Negative_regulation | denotes | siRNAs |
T23649 | 333-338 | Protein | denotes | RUNX3 |
R18308 | T23647 | T23646 | themeOf | RUNX1,siRNAs |
R18309 | T23649 | T23648 | themeOf | RUNX3,siRNAs |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23622 | 18-22 | Protein | denotes | CD4+ |
T23623 | 32-36 | Protein | denotes | CD4+ |
T23624 | 129-139 | Protein | denotes | 2 µM siRNA |
T23625 | 263-268 | Protein | denotes | RUNX1 |
T23626 | 333-338 | Protein | denotes | RUNX3 |
T23627 | 252-258 | Negative_regulation | denotes | siRNAs |
T23628 | 322-328 | Negative_regulation | denotes | siRNAs |
T23629 | 322-328 | Negative_regulation | denotes | siRNAs |
T23630 | 590-593 | Protein | denotes | CD2 |
T23631 | 600-603 | Protein | denotes | CD3 |
T23632 | 609-618 | Protein | denotes | anti-CD28 |
R18303 | T23625 | T23627 | themeOf | RUNX1,siRNAs |
R18304 | T23625 | T23628 | themeOf | RUNX1,siRNAs |
R18305 | T23626 | T23629 | themeOf | RUNX3,siRNAs |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23281 | 18-21 | Protein | denotes | CD4 |
T23282 | 32-35 | Protein | denotes | CD4 |
T23283 | 252-258 | Negative_regulation | denotes | siRNAs |
T23284 | 263-268 | Protein | denotes | RUNX1 |
T23285 | 322-328 | Negative_regulation | denotes | siRNAs |
T23286 | 333-338 | Protein | denotes | RUNX3 |
T23287 | 590-593 | Protein | denotes | CD2 |
T23288 | 600-603 | Protein | denotes | CD3 |
T23289 | 614-618 | Protein | denotes | CD28 |
T23290 | 654-659 | Protein | denotes | AIM-V |
T23291 | 697-701 | Protein | denotes | IL-2 |
R18004 | T23284 | T23283 | themeOf | RUNX1,siRNAs |
R18005 | T23286 | T23285 | themeOf | RUNX3,siRNAs |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23440 | 18-21 | P01730 | denotes | CD4 |
T23441 | 32-35 | P01730 | denotes | CD4 |
T23442 | 263-268 | Q01196 | denotes | RUNX1 |
T23443 | 333-338 | Q13761 | denotes | RUNX3 |
T23444 | 590-593 | P06729 | denotes | CD2 |
T23445 | 600-603 | P20963 | denotes | CD3 |
T23446 | 600-603 | P07766 | denotes | CD3 |
T23447 | 600-603 | P09693 | denotes | CD3 |
T23448 | 600-603 | P04234 | denotes | CD3 |
T23449 | 614-618 | P10747 | denotes | CD28 |
T23450 | 654-657 | P26358 | denotes | AIM |
T23451 | 697-701 | P60568 | denotes | IL-2 |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T23269 | 18-21 | Protein | denotes | CD4 |
T23270 | 32-35 | Protein | denotes | CD4 |
T23271 | 252-258 | Negative_regulation | denotes | siRNAs |
T23272 | 263-268 | Protein | denotes | RUNX1 |
T23273 | 322-328 | Negative_regulation | denotes | siRNAs |
T23274 | 333-338 | Protein | denotes | RUNX3 |
T23275 | 590-593 | Protein | denotes | CD2 |
T23276 | 600-603 | Protein | denotes | CD3 |
T23277 | 614-618 | Protein | denotes | CD28 |
T23278 | 654-659 | Protein | denotes | AIM-V |
T23279 | 697-701 | Protein | denotes | IL-2 |
R18002 | T23272 | T23271 | themeOf | RUNX1,siRNAs |
R18003 | T23274 | T23273 | themeOf | RUNX3,siRNAs |