PMC:2800179 / 4645-6641 JSON TXT

Annnotations TAB JSON ListView MergeView

GO-CC

{"project":"GO-CC","denotations":[{"id":"T2807","span":{"begin":1908,"end":1912},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Preparation of Degraded Carrageenan\nTwo preparations of degraded carrageenan with low, (∼10 kDa; C10), and medium, (∼40 kDa; C40) molecular weight were prepared from native iota-carrageenan extracted from Euchema spinosum (generously provided by Sanofi Biosystems Industry, Boulogne-Billancourt, France). Native carrageenan was dissolved in distilled water (5% w/v) under vigorous stirring and heated to 60°C. Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions. Briefly, for the low molecular weight fraction, carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 15 min at 80°C. After neutralization with NaOH 4N, the solution was ultra filtered through a hollow fibre cartridge with MW cut-off 5 kDa, (Amicon Inc, Beverly, USA). For the medium molecular weight fraction, the carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 30 min at 60°C. After neutralization, the supernatant was ultra filtered (MW cut-off 100 kDa). The filtrate was submitted to a second ultra filtration (MW cut-off 5 kDa). Both preparations of dCGN were precipitated with 4 volumes of 95% ethanol, dried at room temperature and ground to small particles (1 mm in diameter). Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa. The sulphate content of polysaccharides in both fractions was measured following the method of Quemener et al. [22]. Finally, the absence of polysaccharide structure modifications in the two fractions was confirmed using 2H-NMR spectroscopy. The absence of LPS contamination in the two fractions was confirmed using the e-Toxate® kit (Sigma, St Quentin Fallavier, France). Before use in cell culture, the two fractions were dissolved in complete medium during 30 min at 56°C."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T2804","span":{"begin":15,"end":23},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T2805","span":{"begin":56,"end":64},"obj":"http://purl.obolibrary.org/obo/GO_0009056"},{"id":"T2806","span":{"begin":1525,"end":1533},"obj":"http://purl.obolibrary.org/obo/GO_0051923"}],"text":"Preparation of Degraded Carrageenan\nTwo preparations of degraded carrageenan with low, (∼10 kDa; C10), and medium, (∼40 kDa; C40) molecular weight were prepared from native iota-carrageenan extracted from Euchema spinosum (generously provided by Sanofi Biosystems Industry, Boulogne-Billancourt, France). Native carrageenan was dissolved in distilled water (5% w/v) under vigorous stirring and heated to 60°C. Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions. Briefly, for the low molecular weight fraction, carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 15 min at 80°C. After neutralization with NaOH 4N, the solution was ultra filtered through a hollow fibre cartridge with MW cut-off 5 kDa, (Amicon Inc, Beverly, USA). For the medium molecular weight fraction, the carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 30 min at 60°C. After neutralization, the supernatant was ultra filtered (MW cut-off 100 kDa). The filtrate was submitted to a second ultra filtration (MW cut-off 5 kDa). Both preparations of dCGN were precipitated with 4 volumes of 95% ethanol, dried at room temperature and ground to small particles (1 mm in diameter). Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa. The sulphate content of polysaccharides in both fractions was measured following the method of Quemener et al. [22]. Finally, the absence of polysaccharide structure modifications in the two fractions was confirmed using 2H-NMR spectroscopy. The absence of LPS contamination in the two fractions was confirmed using the e-Toxate® kit (Sigma, St Quentin Fallavier, France). Before use in cell culture, the two fractions were dissolved in complete medium during 30 min at 56°C."}

pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

sentences

{"project":"sentences","denotations":[{"id":"T2051","span":{"begin":0,"end":35},"obj":"Sentence"},{"id":"T2052","span":{"begin":36,"end":304},"obj":"Sentence"},{"id":"T2053","span":{"begin":305,"end":540},"obj":"Sentence"},{"id":"T2054","span":{"begin":541,"end":839},"obj":"Sentence"},{"id":"T2055","span":{"begin":840,"end":1064},"obj":"Sentence"},{"id":"T2056","span":{"begin":1065,"end":1140},"obj":"Sentence"},{"id":"T2057","span":{"begin":1141,"end":1291},"obj":"Sentence"},{"id":"T2058","span":{"begin":1292,"end":1520},"obj":"Sentence"},{"id":"T2059","span":{"begin":1521,"end":1637},"obj":"Sentence"},{"id":"T2060","span":{"begin":1638,"end":1762},"obj":"Sentence"},{"id":"T2061","span":{"begin":1763,"end":1893},"obj":"Sentence"},{"id":"T2062","span":{"begin":1894,"end":1996},"obj":"Sentence"},{"id":"T40","span":{"begin":0,"end":35},"obj":"Sentence"},{"id":"T41","span":{"begin":36,"end":304},"obj":"Sentence"},{"id":"T42","span":{"begin":305,"end":409},"obj":"Sentence"},{"id":"T43","span":{"begin":410,"end":540},"obj":"Sentence"},{"id":"T44","span":{"begin":541,"end":688},"obj":"Sentence"},{"id":"T45","span":{"begin":689,"end":839},"obj":"Sentence"},{"id":"T46","span":{"begin":840,"end":985},"obj":"Sentence"},{"id":"T47","span":{"begin":986,"end":1064},"obj":"Sentence"},{"id":"T48","span":{"begin":1065,"end":1140},"obj":"Sentence"},{"id":"T49","span":{"begin":1141,"end":1291},"obj":"Sentence"},{"id":"T50","span":{"begin":1292,"end":1520},"obj":"Sentence"},{"id":"T51","span":{"begin":1521,"end":1637},"obj":"Sentence"},{"id":"T52","span":{"begin":1638,"end":1762},"obj":"Sentence"},{"id":"T53","span":{"begin":1763,"end":1893},"obj":"Sentence"},{"id":"T54","span":{"begin":1894,"end":1996},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Preparation of Degraded Carrageenan\nTwo preparations of degraded carrageenan with low, (∼10 kDa; C10), and medium, (∼40 kDa; C40) molecular weight were prepared from native iota-carrageenan extracted from Euchema spinosum (generously provided by Sanofi Biosystems Industry, Boulogne-Billancourt, France). Native carrageenan was dissolved in distilled water (5% w/v) under vigorous stirring and heated to 60°C. Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions. Briefly, for the low molecular weight fraction, carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 15 min at 80°C. After neutralization with NaOH 4N, the solution was ultra filtered through a hollow fibre cartridge with MW cut-off 5 kDa, (Amicon Inc, Beverly, USA). For the medium molecular weight fraction, the carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 30 min at 60°C. After neutralization, the supernatant was ultra filtered (MW cut-off 100 kDa). The filtrate was submitted to a second ultra filtration (MW cut-off 5 kDa). Both preparations of dCGN were precipitated with 4 volumes of 95% ethanol, dried at room temperature and ground to small particles (1 mm in diameter). Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa. The sulphate content of polysaccharides in both fractions was measured following the method of Quemener et al. [22]. Finally, the absence of polysaccharide structure modifications in the two fractions was confirmed using 2H-NMR spectroscopy. The absence of LPS contamination in the two fractions was confirmed using the e-Toxate® kit (Sigma, St Quentin Fallavier, France). Before use in cell culture, the two fractions were dissolved in complete medium during 30 min at 56°C."}

bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T2808","span":{"begin":1856,"end":1861},"obj":"Protein"},{"id":"T2809","span":{"begin":1863,"end":1873},"obj":"Protein"}],"text":"Preparation of Degraded Carrageenan\nTwo preparations of degraded carrageenan with low, (∼10 kDa; C10), and medium, (∼40 kDa; C40) molecular weight were prepared from native iota-carrageenan extracted from Euchema spinosum (generously provided by Sanofi Biosystems Industry, Boulogne-Billancourt, France). Native carrageenan was dissolved in distilled water (5% w/v) under vigorous stirring and heated to 60°C. Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions. Briefly, for the low molecular weight fraction, carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 15 min at 80°C. After neutralization with NaOH 4N, the solution was ultra filtered through a hollow fibre cartridge with MW cut-off 5 kDa, (Amicon Inc, Beverly, USA). For the medium molecular weight fraction, the carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 30 min at 60°C. After neutralization, the supernatant was ultra filtered (MW cut-off 100 kDa). The filtrate was submitted to a second ultra filtration (MW cut-off 5 kDa). Both preparations of dCGN were precipitated with 4 volumes of 95% ethanol, dried at room temperature and ground to small particles (1 mm in diameter). Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa. The sulphate content of polysaccharides in both fractions was measured following the method of Quemener et al. [22]. Finally, the absence of polysaccharide structure modifications in the two fractions was confirmed using 2H-NMR spectroscopy. The absence of LPS contamination in the two fractions was confirmed using the e-Toxate® kit (Sigma, St Quentin Fallavier, France). Before use in cell culture, the two fractions were dissolved in complete medium during 30 min at 56°C."}