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PMC:2800179 / 4645-6641 JSONTXT

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GO-CC

Id Subject Object Predicate Lexical cue
T2807 1908-1912 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

GO-BP

Id Subject Object Predicate Lexical cue
T2804 15-23 http://purl.obolibrary.org/obo/GO_0009056 denotes Degraded
T2805 56-64 http://purl.obolibrary.org/obo/GO_0009056 denotes degraded
T2806 1525-1533 http://purl.obolibrary.org/obo/GO_0051923 denotes sulphate

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T2063 0-11 NN denotes Preparation
T2064 12-14 IN denotes of
T2065 15-23 NNP denotes Degraded
T2066 24-35 NNP denotes Carrageenan
T2067 36-39 CD denotes Two
T2068 40-52 NN denotes preparations
T2069 53-55 IN denotes of
T2070 56-64 VB denotes degraded
T2071 65-76 NN denotes carrageenan
T2072 77-81 IN denotes with
T2073 82-85 JJ denotes low
T2074 85-86 -COMMA- denotes ,
T2075 87-88 -LRB- denotes (
T2076 88-91 CD denotes ∼10
T2077 92-95 NN denotes kDa
T2078 95-96 -COLON- denotes ;
T2079 97-100 NN denotes C10
T2080 100-101 -RRB- denotes )
T2081 101-102 -COMMA- denotes ,
T2082 103-106 CC denotes and
T2083 107-113 NN denotes medium
T2084 113-114 -COMMA- denotes ,
T2085 115-116 -LRB- denotes (
T2086 116-119 CD denotes ∼40
T2087 120-123 NN denotes kDa
T2088 123-124 -COLON- denotes ;
T2089 125-128 NN denotes C40
T2090 128-129 -RRB- denotes )
T2091 130-139 JJ denotes molecular
T2092 140-146 NN denotes weight
T2093 147-151 VB denotes were
T2094 152-160 VB denotes prepared
T2095 161-165 IN denotes from
T2096 166-172 JJ denotes native
T2097 173-189 NN denotes iota-carrageenan
T2098 190-199 VB denotes extracted
T2099 200-204 IN denotes from
T2100 205-212 NNP denotes Euchema
T2101 213-221 NN denotes spinosum
T2102 222-223 -LRB- denotes (
T2103 223-233 RB denotes generously
T2104 234-242 VB denotes provided
T2105 243-245 IN denotes by
T2106 246-252 NNP denotes Sanofi
T2107 253-263 NNP denotes Biosystems
T2108 264-272 NNP denotes Industry
T2109 272-273 -COMMA- denotes ,
T2110 274-294 NNP denotes Boulogne-Billancourt
T2111 294-295 -COMMA- denotes ,
T2112 296-302 NNP denotes France
T2113 302-303 -RRB- denotes )
T2114 305-311 JJ denotes Native
T2115 312-323 NN denotes carrageenan
T2116 324-327 VB denotes was
T2117 328-337 VB denotes dissolved
T2118 338-340 IN denotes in
T2119 341-350 JJ denotes distilled
T2120 351-356 NN denotes water
T2121 357-358 -LRB- denotes (
T2122 358-359 CD denotes 5
T2123 359-360 NN denotes %
T2124 361-364 NN denotes w/v
T2125 364-365 -RRB- denotes )
T2126 366-371 IN denotes under
T2127 372-380 JJ denotes vigorous
T2128 381-389 VB denotes stirring
T2129 390-393 CC denotes and
T2130 394-400 VB denotes heated
T2131 401-403 TO denotes to
T2132 404-409 NNP denotes 60°C.
T2133 410-414 NNP denotes Then
T2134 414-415 -COMMA- denotes ,
T2135 416-419 DT denotes the
T2136 420-431 NN denotes carrageenan
T2137 432-440 NN denotes solution
T2138 441-444 VB denotes was
T2139 445-454 VB denotes submitted
T2140 455-457 TO denotes to
T2141 458-461 CD denotes two
T2142 462-471 JJ denotes different
T2143 472-482 NN denotes treatments
T2144 483-485 TO denotes to
T2145 486-492 VB denotes obtain
T2146 493-497 CC denotes both
T2147 498-501 JJ denotes low
T2148 502-505 CC denotes and
T2149 506-512 JJ denotes medium
T2150 513-522 JJ denotes molecular
T2151 523-529 NN denotes weight
T2152 530-539 NN denotes fractions
T2153 541-548 RB denotes Briefly
T2154 548-549 -COMMA- denotes ,
T2155 550-553 IN denotes for
T2156 554-557 DT denotes the
T2157 558-561 JJ denotes low
T2158 562-571 JJ denotes molecular
T2159 572-578 NN denotes weight
T2160 579-587 NN denotes fraction
T2161 587-588 -COMMA- denotes ,
T2162 589-600 NN denotes carrageenan
T2163 601-609 NN denotes solution
T2164 610-613 VB denotes was
T2165 614-624 VB denotes hydrolyzed
T2166 625-629 IN denotes with
T2167 630-633 CD denotes 0.3
T2168 633-634 NN denotes %
T2169 635-636 -LRB- denotes (
T2170 636-639 NN denotes v/v
T2171 639-640 -RRB- denotes )
T2172 641-653 VB denotes concentrated
T2173 654-663 JJ denotes sulphuric
T2174 664-668 NN denotes acid
T2175 669-672 IN denotes for
T2176 673-675 CD denotes 15
T2177 676-679 NN denotes min
T2178 680-682 IN denotes at
T2179 683-688 NNP denotes 80°C.
T2180 689-694 NNP denotes After
T2181 695-709 NN denotes neutralization
T2182 710-714 IN denotes with
T2183 715-719 NN denotes NaOH
T2184 720-722 NN denotes 4N
T2185 722-723 -COMMA- denotes ,
T2186 724-727 DT denotes the
T2187 728-736 NN denotes solution
T2188 737-740 VB denotes was
T2189 741-746 NN denotes ultra
T2190 747-755 VB denotes filtered
T2191 756-763 IN denotes through
T2192 764-765 DT denotes a
T2193 766-772 JJ denotes hollow
T2194 773-778 NN denotes fibre
T2195 779-788 NN denotes cartridge
T2196 789-793 IN denotes with
T2197 794-796 NN denotes MW
T2198 797-804 JJ denotes cut-off
T2199 805-806 CD denotes 5
T2200 807-810 NN denotes kDa
T2201 810-811 -COMMA- denotes ,
T2202 812-813 -LRB- denotes (
T2203 813-819 NNP denotes Amicon
T2204 820-823 NNP denotes Inc
T2205 823-824 -COMMA- denotes ,
T2206 825-832 NNP denotes Beverly
T2207 832-833 -COMMA- denotes ,
T2208 834-837 NNP denotes USA
T2209 837-838 -RRB- denotes )
T2210 840-843 IN denotes For
T2211 844-847 DT denotes the
T2212 848-854 NN denotes medium
T2213 855-864 JJ denotes molecular
T2214 865-871 NN denotes weight
T2215 872-880 NN denotes fraction
T2216 880-881 -COMMA- denotes ,
T2217 882-885 DT denotes the
T2218 886-897 NN denotes carrageenan
T2219 898-906 NN denotes solution
T2220 907-910 VB denotes was
T2221 911-921 VB denotes hydrolyzed
T2222 922-926 IN denotes with
T2223 927-930 CD denotes 0.3
T2224 930-931 NN denotes %
T2225 932-933 -LRB- denotes (
T2226 933-936 NN denotes v/v
T2227 936-937 -RRB- denotes )
T2228 938-950 VB denotes concentrated
T2229 951-960 JJ denotes sulphuric
T2230 961-965 NN denotes acid
T2231 966-969 IN denotes for
T2232 970-972 CD denotes 30
T2233 973-976 NN denotes min
T2234 977-979 IN denotes at
T2235 980-985 NNP denotes 60°C.
T2236 986-991 IN denotes After
T2237 992-1006 NN denotes neutralization
T2238 1006-1007 -COMMA- denotes ,
T2239 1008-1011 DT denotes the
T2240 1012-1023 NN denotes supernatant
T2241 1024-1027 VB denotes was
T2242 1028-1033 AFX denotes ultra
T2243 1034-1042 VB denotes filtered
T2244 1043-1044 -LRB- denotes (
T2245 1044-1046 NN denotes MW
T2246 1047-1054 JJ denotes cut-off
T2247 1055-1058 CD denotes 100
T2248 1059-1062 NN denotes kDa
T2249 1062-1063 -RRB- denotes )
T2250 1065-1068 DT denotes The
T2251 1069-1077 NN denotes filtrate
T2252 1078-1081 VB denotes was
T2253 1082-1091 VB denotes submitted
T2254 1092-1094 TO denotes to
T2255 1095-1096 DT denotes a
T2256 1097-1103 JJ denotes second
T2257 1104-1109 NN denotes ultra
T2258 1110-1120 NN denotes filtration
T2259 1121-1122 -LRB- denotes (
T2260 1122-1124 NN denotes MW
T2261 1125-1132 NN denotes cut-off
T2262 1133-1134 CD denotes 5
T2263 1135-1138 NN denotes kDa
T2264 1138-1139 -RRB- denotes )
T2265 1141-1145 DT denotes Both
T2266 1146-1158 NN denotes preparations
T2267 1159-1161 IN denotes of
T2268 1162-1166 NN denotes dCGN
T2269 1167-1171 VB denotes were
T2270 1172-1184 VB denotes precipitated
T2271 1185-1189 IN denotes with
T2272 1190-1191 CD denotes 4
T2273 1192-1199 NN denotes volumes
T2274 1200-1202 IN denotes of
T2275 1203-1205 CD denotes 95
T2276 1205-1206 NN denotes %
T2277 1207-1214 NN denotes ethanol
T2278 1214-1215 -COMMA- denotes ,
T2279 1216-1221 VB denotes dried
T2280 1222-1224 IN denotes at
T2281 1225-1229 NN denotes room
T2282 1230-1241 NN denotes temperature
T2283 1242-1245 CC denotes and
T2284 1246-1252 NN denotes ground
T2285 1253-1255 TO denotes to
T2286 1256-1261 JJ denotes small
T2287 1262-1271 NN denotes particles
T2288 1272-1273 -LRB- denotes (
T2289 1273-1274 CD denotes 1
T2290 1275-1277 NN denotes mm
T2291 1278-1280 IN denotes in
T2292 1281-1289 NN denotes diameter
T2293 1289-1290 -RRB- denotes )
T2294 1292-1297 VB denotes Using
T2295 1298-1312 NN denotes gel-permeation
T2296 1313-1327 NN denotes chromatography
T2297 1328-1330 IN denotes in
T2298 1331-1342 NN denotes combination
T2299 1343-1347 IN denotes with
T2300 1348-1353 JJ denotes light
T2301 1354-1364 NN denotes scattering
T2302 1365-1377 NN denotes measurements
T2303 1378-1379 -LRB- denotes (
T2304 1379-1382 VB denotes see
T2305 1383-1389 NNP denotes Viebke
T2306 1390-1392 FW denotes et
T2307 1393-1396 FW denotes al.
T2308 1397-1398 -LRB- denotes [
T2309 1398-1400 CD denotes 21
T2310 1400-1401 -RRB- denotes ]
T2311 1401-1402 -RRB- denotes )
T2312 1402-1403 -COMMA- denotes ,
T2313 1404-1406 PRP denotes it
T2314 1407-1410 VB denotes was
T2315 1411-1420 VB denotes confirmed
T2316 1421-1425 IN denotes that
T2317 1426-1429 DT denotes the
T2318 1430-1433 JJ denotes low
T2319 1434-1442 NN denotes fraction
T2320 1443-1446 VB denotes had
T2321 1447-1449 DT denotes an
T2322 1450-1457 JJ denotes average
T2323 1458-1467 JJ denotes molecular
T2324 1468-1474 NN denotes weight
T2325 1475-1477 IN denotes of
T2326 1478-1480 CD denotes 10
T2327 1481-1484 NN denotes kDa
T2328 1484-1485 -COMMA- denotes ,
T2329 1486-1489 CC denotes and
T2330 1490-1493 DT denotes the
T2331 1494-1500 NN denotes medium
T2332 1501-1509 NN denotes fraction
T2333 1510-1512 IN denotes of
T2334 1513-1515 CD denotes 40
T2335 1516-1519 NN denotes kDa
T2336 1521-1524 DT denotes The
T2337 1525-1533 NN denotes sulphate
T2338 1534-1541 NN denotes content
T2339 1542-1544 IN denotes of
T2340 1545-1560 NN denotes polysaccharides
T2341 1561-1563 IN denotes in
T2342 1564-1568 DT denotes both
T2343 1569-1578 NN denotes fractions
T2344 1579-1582 VB denotes was
T2345 1583-1591 VB denotes measured
T2346 1592-1601 VB denotes following
T2347 1602-1605 DT denotes the
T2348 1606-1612 NN denotes method
T2349 1613-1615 IN denotes of
T2350 1616-1624 NNP denotes Quemener
T2351 1625-1627 FW denotes et
T2352 1628-1631 FW denotes al.
T2353 1632-1633 -LRB- denotes [
T2354 1633-1635 CD denotes 22
T2355 1635-1636 -RRB- denotes ]
T2356 1638-1645 RB denotes Finally
T2357 1645-1646 -COMMA- denotes ,
T2358 1647-1650 DT denotes the
T2359 1651-1658 NN denotes absence
T2360 1659-1661 IN denotes of
T2361 1662-1676 NN denotes polysaccharide
T2362 1677-1686 NN denotes structure
T2363 1687-1700 NN denotes modifications
T2364 1701-1703 IN denotes in
T2365 1704-1707 DT denotes the
T2366 1708-1711 CD denotes two
T2367 1712-1721 NN denotes fractions
T2368 1722-1725 VB denotes was
T2369 1726-1735 VB denotes confirmed
T2370 1736-1741 VB denotes using
T2371 1742-1748 NN denotes 2H-NMR
T2372 1749-1761 NN denotes spectroscopy
T2373 1763-1766 DT denotes The
T2374 1767-1774 NN denotes absence
T2375 1775-1777 IN denotes of
T2376 1778-1781 NN denotes LPS
T2377 1782-1795 NN denotes contamination
T2378 1796-1798 IN denotes in
T2379 1799-1802 DT denotes the
T2380 1803-1806 CD denotes two
T2381 1807-1816 NN denotes fractions
T2382 1817-1820 VB denotes was
T2383 1821-1830 VB denotes confirmed
T2384 1831-1836 VB denotes using
T2385 1837-1840 DT denotes the
T2386 1841-1850 NN denotes e-Toxate®
T2387 1851-1854 NN denotes kit
T2388 1855-1856 -LRB- denotes (
T2389 1856-1861 NNP denotes Sigma
T2390 1861-1862 -COMMA- denotes ,
T2391 1863-1865 NNP denotes St
T2392 1866-1873 NNP denotes Quentin
T2393 1874-1883 NNP denotes Fallavier
T2394 1883-1884 -COMMA- denotes ,
T2395 1885-1891 NNP denotes France
T2396 1891-1892 -RRB- denotes )
T2397 1894-1900 IN denotes Before
T2398 1901-1904 NN denotes use
T2399 1905-1907 IN denotes in
T2400 1908-1912 NN denotes cell
T2401 1913-1920 NN denotes culture
T2402 1920-1921 -COMMA- denotes ,
T2403 1922-1925 DT denotes the
T2404 1926-1929 CD denotes two
T2405 1930-1939 NN denotes fractions
T2406 1940-1944 VB denotes were
T2407 1945-1954 VB denotes dissolved
T2408 1955-1957 IN denotes in
T2409 1958-1966 JJ denotes complete
T2410 1967-1973 NN denotes medium
T2411 1974-1980 IN denotes during
T2412 1981-1983 CD denotes 30
T2413 1984-1987 NN denotes min
T2414 1988-1990 IN denotes at
T2415 1991-1995 NN denotes 56°C
R1776 T2063 T2064 arg1Of Preparation,of
R1777 T2066 T2064 arg2Of Carrageenan,of
R1778 T2066 T2065 arg1Of Carrageenan,Degraded
R1779 T2068 T2067 arg1Of preparations,Two
R1780 T2068 T2069 arg1Of preparations,of
R1781 T2068 T2093 modOf preparations,were
R1784 T2071 T2069 arg2Of carrageenan,of
R1786 T2073 T2075 arg1Of low,(
R1787 T2073 T2082 arg1Of low,and
R1788 T2077 T2075 arg2Of kDa,(
R1789 T2077 T2076 arg1Of kDa,∼10
R1790 T2077 T2078 arg1Of kDa,;
R1791 T2079 T2078 arg2Of C10,;
R1792 T2080 T2075 arg3Of ),(
R1794 T2082 T2081 arg1Of and,","
R1795 T2083 T2082 arg2Of medium,and
R1796 T2087 T2085 arg2Of kDa,(
R1797 T2087 T2086 arg1Of kDa,∼40
R1798 T2087 T2088 arg1Of kDa,;
R1799 T2089 T2088 arg2Of C40,;
R1800 T2090 T2085 arg3Of ),(
R1801 T2092 T2085 arg1Of weight,(
R1802 T2092 T2091 arg1Of weight,molecular
R1803 T2092 T2093 arg1Of weight,were
R1804 T2092 T2094 arg2Of weight,prepared
R1805 T2094 T2084 arg1Of prepared,","
R1806 T2094 T2093 arg2Of prepared,were
R1807 T2094 T2095 arg1Of prepared,from
R1808 T2097 T2095 arg2Of iota-carrageenan,from
R1809 T2097 T2096 arg1Of iota-carrageenan,native
R1810 T2097 T2098 arg2Of iota-carrageenan,extracted
R1811 T2098 T2099 arg1Of extracted,from
R1812 T2101 T2099 arg2Of spinosum,from
R1813 T2101 T2100 arg1Of spinosum,Euchema
R1814 T2101 T2102 arg1Of spinosum,(
R1815 T2104 T2102 arg2Of provided,(
R1816 T2104 T2103 arg1Of provided,generously
R1817 T2108 T2104 arg1Of Industry,provided
R1818 T2108 T2105 arg2Of Industry,by
R1819 T2108 T2106 arg1Of Industry,Sanofi
R1820 T2108 T2107 arg1Of Industry,Biosystems
R1821 T2108 T2109 arg1Of Industry,","
R1822 T2110 T2109 arg2Of Boulogne-Billancourt,","
R1823 T2110 T2111 arg1Of Boulogne-Billancourt,","
R1824 T2112 T2111 arg2Of France,","
R1825 T2113 T2102 arg3Of ),(
R1826 T2115 T2114 arg1Of carrageenan,Native
R1827 T2115 T2116 arg1Of carrageenan,was
R1828 T2115 T2117 arg2Of carrageenan,dissolved
R1829 T2115 T2130 arg2Of carrageenan,heated
R1830 T2117 T2118 arg1Of dissolved,in
R1831 T2117 T2129 arg1Of dissolved,and
R1832 T2120 T2118 arg2Of water,in
R1833 T2120 T2119 arg1Of water,distilled
R1834 T2120 T2121 arg1Of water,(
R1835 T2120 T2126 arg1Of water,under
R1836 T2122 T2123 arg1Of 5,%
R1837 T2124 T2121 arg2Of w/v,(
R1838 T2124 T2122 arg1Of w/v,5
R1839 T2125 T2121 arg3Of ),(
R1840 T2128 T2126 arg2Of stirring,under
R1841 T2128 T2127 arg1Of stirring,vigorous
R1842 T2129 T2116 arg2Of and,was
R1843 T2130 T2129 arg2Of heated,and
R1844 T2130 T2131 arg1Of heated,to
R1845 T2133 T2131 arg2Of Then,to
R1846 T2133 T2132 arg1Of Then,60°C.
R1847 T2137 T2135 arg1Of solution,the
R1848 T2137 T2136 arg1Of solution,carrageenan
R1849 T2137 T2138 arg1Of solution,was
R1850 T2137 T2139 arg2Of solution,submitted
R1851 T2139 T2116 modOf submitted,was
R1852 T2139 T2134 arg1Of submitted,","
R1853 T2139 T2138 arg2Of submitted,was
R1854 T2139 T2140 arg1Of submitted,to
R1855 T2143 T2140 arg2Of treatments,to
R1856 T2143 T2141 arg1Of treatments,two
R1857 T2143 T2142 arg1Of treatments,different
R1858 T2145 T2139 arg3Of obtain,submitted
R1859 T2145 T2144 arg1Of obtain,to
R1860 T2147 T2148 arg1Of low,and
R1861 T2148 T2146 arg1Of and,both
R1862 T2149 T2148 arg2Of medium,and
R1863 T2152 T2145 arg2Of fractions,obtain
R1864 T2152 T2147 arg1Of fractions,low
R1865 T2152 T2149 arg1Of fractions,medium
R1866 T2152 T2150 arg1Of fractions,molecular
R1867 T2152 T2151 arg1Of fractions,weight
R1868 T2160 T2155 arg2Of fraction,for
R1869 T2160 T2156 arg1Of fraction,the
R1870 T2160 T2157 arg1Of fraction,low
R1871 T2160 T2158 arg1Of fraction,molecular
R1872 T2160 T2159 arg1Of fraction,weight
R1873 T2163 T2162 arg1Of solution,carrageenan
R1874 T2163 T2164 arg1Of solution,was
R1875 T2163 T2165 arg2Of solution,hydrolyzed
R1876 T2165 T2164 arg2Of hydrolyzed,was
R1877 T2165 T2166 arg1Of hydrolyzed,with
R1878 T2168 T2166 arg2Of %,with
R1879 T2168 T2167 arg1Of %,0.3
R1880 T2168 T2169 arg1Of %,(
R1881 T2168 T2172 arg2Of %,concentrated
R1882 T2170 T2169 arg2Of v/v,(
R1883 T2171 T2169 arg3Of ),(
R1884 T2174 T2172 arg1Of acid,concentrated
R1885 T2174 T2173 arg1Of acid,sulphuric
R1886 T2174 T2175 arg1Of acid,for
R1887 T2174 T2178 arg1Of acid,at
R1888 T2177 T2175 arg2Of min,for
R1889 T2177 T2176 arg1Of min,15
R1890 T2180 T2179 arg1Of After,80°C.
R1891 T2181 T2178 arg2Of neutralization,at
R1892 T2181 T2180 arg1Of neutralization,After
R1893 T2181 T2182 arg1Of neutralization,with
R1894 T2184 T2182 arg2Of 4N,with
R1895 T2184 T2183 arg1Of 4N,NaOH
R1896 T2187 T2186 arg1Of solution,the
R1897 T2187 T2188 arg1Of solution,was
R1898 T2188 T2153 arg1Of was,Briefly
R1899 T2188 T2154 arg1Of was,","
R1900 T2188 T2155 arg1Of was,for
R1901 T2188 T2161 arg1Of was,","
R1902 T2188 T2164 modOf was,was
R1903 T2188 T2185 arg1Of was,","
R1904 T2189 T2188 arg2Of ultra,was
R1905 T2189 T2190 arg2Of ultra,filtered
R1906 T2189 T2201 arg1Of ultra,","
R1907 T2189 T2202 arg1Of ultra,(
R1908 T2190 T2191 arg1Of filtered,through
R1909 T2195 T2191 arg2Of cartridge,through
R1910 T2195 T2192 arg1Of cartridge,a
R1911 T2195 T2193 arg1Of cartridge,hollow
R1912 T2195 T2194 arg1Of cartridge,fibre
R1913 T2195 T2196 arg1Of cartridge,with
R1914 T2200 T2196 arg2Of kDa,with
R1915 T2200 T2197 arg1Of kDa,MW
R1916 T2200 T2198 arg1Of kDa,cut-off
R1917 T2200 T2199 arg1Of kDa,5
R1918 T2204 T2202 arg2Of Inc,(
R1919 T2204 T2203 arg1Of Inc,Amicon
R1920 T2204 T2205 arg1Of Inc,","
R1921 T2206 T2205 arg2Of Beverly,","
R1922 T2206 T2207 arg1Of Beverly,","
R1923 T2208 T2207 arg2Of USA,","
R1924 T2209 T2202 arg3Of ),(
R1925 T2215 T2211 arg1Of fraction,the
R1926 T2215 T2212 arg1Of fraction,medium
R1927 T2215 T2213 arg1Of fraction,molecular
R1928 T2215 T2214 arg1Of fraction,weight
R1929 T2215 T2228 arg1Of fraction,concentrated
R1930 T2219 T2217 arg1Of solution,the
R1931 T2219 T2218 arg1Of solution,carrageenan
R1932 T2219 T2220 arg1Of solution,was
R1933 T2219 T2221 arg2Of solution,hydrolyzed
R1934 T2221 T2220 arg2Of hydrolyzed,was
R1935 T2221 T2222 arg1Of hydrolyzed,with
R1936 T2224 T2222 arg2Of %,with
R1937 T2224 T2223 arg1Of %,0.3
R1938 T2224 T2225 arg1Of %,(
R1939 T2226 T2225 arg2Of v/v,(
R1940 T2227 T2225 arg3Of ),(
R1941 T2228 T2210 arg1Of concentrated,For
R1942 T2228 T2216 arg1Of concentrated,","
R1943 T2228 T2220 modOf concentrated,was
R1944 T2228 T2244 arg1Of concentrated,(
R1945 T2230 T2228 arg2Of acid,concentrated
R1946 T2230 T2229 arg1Of acid,sulphuric
R1947 T2230 T2231 arg1Of acid,for
R1948 T2230 T2234 arg1Of acid,at
R1949 T2230 T2243 arg2Of acid,filtered
R1950 T2233 T2231 arg2Of min,for
R1951 T2233 T2232 arg1Of min,30
R1952 T2235 T2234 arg2Of 60°C.,at
R1953 T2235 T2236 arg1Of 60°C.,After
R1954 T2237 T2236 arg2Of neutralization,After
R1955 T2240 T2239 arg1Of supernatant,the
R1956 T2240 T2241 arg1Of supernatant,was
R1957 T2240 T2242 arg1Of supernatant,ultra
R1958 T2241 T2238 arg1Of was,","
R1959 T2242 T2241 arg2Of ultra,was
R1960 T2243 T2241 arg3Of filtered,was
R1961 T2245 T2244 arg2Of MW,(
R1962 T2245 T2246 arg1Of MW,cut-off
R1963 T2246 T2248 arg1Of cut-off,kDa
R1964 T2248 T2247 arg1Of kDa,100
R1965 T2249 T2244 arg3Of ),(
R1966 T2251 T2250 arg1Of filtrate,The
R1967 T2251 T2252 arg1Of filtrate,was
R1968 T2251 T2253 arg2Of filtrate,submitted
R1969 T2253 T2252 arg2Of submitted,was
R1970 T2253 T2254 arg1Of submitted,to
R1971 T2258 T2254 arg2Of filtration,to
R1972 T2258 T2255 arg1Of filtration,a
R1973 T2258 T2256 arg1Of filtration,second
R1974 T2258 T2257 arg1Of filtration,ultra
R1975 T2258 T2259 arg1Of filtration,(
R1976 T2263 T2259 arg2Of kDa,(
R1977 T2263 T2260 arg1Of kDa,MW
R1978 T2263 T2261 arg1Of kDa,cut-off
R1979 T2263 T2262 arg1Of kDa,5
R1980 T2264 T2259 arg3Of ),(
R1981 T2266 T2265 arg1Of preparations,Both
R1982 T2266 T2267 arg1Of preparations,of
R1983 T2266 T2269 arg1Of preparations,were
R1984 T2266 T2270 arg2Of preparations,precipitated
R1985 T2268 T2267 arg2Of dCGN,of
R1986 T2270 T2269 arg2Of precipitated,were
R1987 T2270 T2271 arg1Of precipitated,with
R1988 T2273 T2271 arg2Of volumes,with
R1989 T2273 T2272 arg1Of volumes,4
R1990 T2273 T2274 arg1Of volumes,of
R1991 T2273 T2279 arg2Of volumes,dried
R1992 T2275 T2276 arg1Of 95,%
R1993 T2277 T2274 arg2Of ethanol,of
R1994 T2277 T2275 arg1Of ethanol,95
R1995 T2279 T2278 arg1Of dried,","
R1996 T2279 T2280 arg1Of dried,at
R1997 T2282 T2281 arg1Of temperature,room
R1998 T2282 T2283 arg1Of temperature,and
R1999 T2283 T2280 arg2Of and,at
R2000 T2284 T2283 arg2Of ground,and
R2001 T2284 T2285 arg1Of ground,to
R2002 T2287 T2285 arg2Of particles,to
R2003 T2287 T2286 arg1Of particles,small
R2004 T2287 T2288 arg1Of particles,(
R2005 T2290 T2288 arg2Of mm,(
R2006 T2290 T2289 arg1Of mm,1
R2007 T2290 T2291 arg1Of mm,in
R2008 T2292 T2291 arg2Of diameter,in
R2009 T2293 T2288 arg3Of ),(
R2010 T2296 T2294 arg1Of chromatography,Using
R2011 T2296 T2295 arg1Of chromatography,gel-permeation
R2012 T2296 T2297 arg1Of chromatography,in
R2013 T2296 T2299 arg1Of chromatography,with
R2014 T2296 T2304 arg1Of chromatography,see
R2015 T2298 T2297 arg2Of combination,in
R2016 T2302 T2299 arg2Of measurements,with
R2017 T2302 T2300 arg1Of measurements,light
R2018 T2302 T2301 arg1Of measurements,scattering
R2019 T2304 T2303 arg1Of see,(
R2020 T2307 T2305 arg1Of al.,Viebke
R2021 T2307 T2306 arg1Of al.,et
R2022 T2307 T2308 arg1Of al.,[
R2023 T2307 T2311 arg1Of al.,)
R2024 T2307 T2312 arg1Of al.,","
R2025 T2307 T2314 arg1Of al.,was
R2026 T2307 T2315 arg2Of al.,confirmed
R2027 T2309 T2308 arg2Of 21,[
R2028 T2310 T2308 arg3Of ],[
R2029 T2313 T2312 arg2Of it,","
R2030 T2315 T2314 arg2Of confirmed,was
R2031 T2316 T2315 arg3Of that,confirmed
R2032 T2319 T2317 arg1Of fraction,the
R2033 T2319 T2318 arg1Of fraction,low
R2034 T2319 T2320 arg1Of fraction,had
R2035 T2320 T2304 arg2Of had,see
R2036 T2320 T2314 modOf had,was
R2037 T2324 T2321 arg1Of weight,an
R2038 T2324 T2322 arg1Of weight,average
R2039 T2324 T2323 arg1Of weight,molecular
R2040 T2324 T2325 arg1Of weight,of
R2041 T2324 T2329 arg1Of weight,and
R2042 T2327 T2325 arg2Of kDa,of
R2043 T2327 T2326 arg1Of kDa,10
R2044 T2329 T2320 arg2Of and,had
R2045 T2329 T2328 arg1Of and,","
R2046 T2332 T2329 arg2Of fraction,and
R2047 T2332 T2330 arg1Of fraction,the
R2048 T2332 T2331 arg1Of fraction,medium
R2049 T2332 T2333 arg1Of fraction,of
R2050 T2335 T2333 arg2Of kDa,of
R2051 T2335 T2334 arg1Of kDa,40
R2052 T2338 T2336 arg1Of content,The
R2053 T2338 T2337 arg1Of content,sulphate
R2054 T2338 T2339 arg1Of content,of
R2055 T2338 T2341 arg1Of content,in
R2056 T2338 T2344 arg1Of content,was
R2057 T2338 T2345 arg2Of content,measured
R2058 T2340 T2339 arg2Of polysaccharides,of
R2059 T2343 T2341 arg2Of fractions,in
R2060 T2343 T2342 arg1Of fractions,both
R2061 T2345 T2344 arg2Of measured,was
R2062 T2345 T2346 arg1Of measured,following
R2063 T2348 T2346 arg2Of method,following
R2064 T2348 T2347 arg1Of method,the
R2065 T2348 T2349 arg1Of method,of
R2066 T2352 T2349 arg2Of al.,of
R2067 T2352 T2350 arg1Of al.,Quemener
R2068 T2352 T2351 arg1Of al.,et
R2069 T2352 T2353 arg1Of al.,[
R2070 T2354 T2353 arg2Of 22,[
R2071 T2355 T2353 arg3Of ],[
R2072 T2359 T2358 arg1Of absence,the
R2073 T2359 T2360 arg1Of absence,of
R2074 T2359 T2368 arg1Of absence,was
R2075 T2359 T2369 arg2Of absence,confirmed
R2076 T2363 T2360 arg2Of modifications,of
R2077 T2363 T2361 arg1Of modifications,polysaccharide
R2078 T2363 T2362 arg1Of modifications,structure
R2079 T2363 T2364 arg1Of modifications,in
R2080 T2367 T2364 arg2Of fractions,in
R2081 T2367 T2365 arg1Of fractions,the
R2082 T2367 T2366 arg1Of fractions,two
R2083 T2369 T2356 arg1Of confirmed,Finally
R2084 T2369 T2357 arg1Of confirmed,","
R2085 T2369 T2368 arg2Of confirmed,was
R2086 T2370 T2369 arg3Of using,confirmed
R2087 T2372 T2370 arg2Of spectroscopy,using
R2088 T2372 T2371 arg1Of spectroscopy,2H-NMR
R2089 T2374 T2373 arg1Of absence,The
R2090 T2374 T2375 arg1Of absence,of
R2091 T2374 T2378 arg1Of absence,in
R2092 T2374 T2382 arg1Of absence,was
R2093 T2374 T2383 arg2Of absence,confirmed
R2094 T2377 T2375 arg2Of contamination,of
R2095 T2377 T2376 arg1Of contamination,LPS
R2096 T2381 T2378 arg2Of fractions,in
R2097 T2381 T2379 arg1Of fractions,the
R2098 T2381 T2380 arg1Of fractions,two
R2099 T2383 T2382 arg2Of confirmed,was
R2100 T2384 T2383 arg3Of using,confirmed
R2101 T2384 T2388 arg1Of using,(
R2102 T2387 T2384 arg2Of kit,using
R2103 T2387 T2385 arg1Of kit,the
R2104 T2387 T2386 arg1Of kit,e-Toxate®
R2105 T2389 T2388 arg2Of Sigma,(
R2106 T2389 T2390 arg1Of Sigma,","
R2107 T2393 T2390 arg2Of Fallavier,","
R2108 T2393 T2391 arg1Of Fallavier,St
R2109 T2393 T2392 arg1Of Fallavier,Quentin
R2110 T2393 T2394 arg1Of Fallavier,","
R2111 T2395 T2394 arg2Of France,","
R2112 T2396 T2388 arg3Of ),(
R2113 T2398 T2397 arg2Of use,Before
R2114 T2398 T2399 arg1Of use,in
R2115 T2401 T2399 arg2Of culture,in
R2116 T2401 T2400 arg1Of culture,cell
R2117 T2405 T2403 arg1Of fractions,the
R2118 T2405 T2404 arg1Of fractions,two
R2119 T2405 T2406 arg1Of fractions,were
R2120 T2405 T2407 arg2Of fractions,dissolved
R2121 T2407 T2397 arg1Of dissolved,Before
R2122 T2407 T2402 arg1Of dissolved,","
R2123 T2407 T2406 arg2Of dissolved,were
R2124 T2407 T2408 arg1Of dissolved,in
R2125 T2407 T2411 arg1Of dissolved,during
R2126 T2410 T2408 arg2Of medium,in
R2127 T2410 T2409 arg1Of medium,complete
R2128 T2413 T2411 arg2Of min,during
R2129 T2413 T2412 arg1Of min,30
R2130 T2413 T2414 arg1Of min,at
R2131 T2415 T2414 arg2Of 56°C,at
R1782 T2071 T2070 arg2Of carrageenan,degraded
R1783 T2071 T2072 arg1Of carrageenan,with
R1785 T2073 T2074 arg1Of low,","
R1793 T2082 T2072 arg2Of and,with

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T2416 0-11 NNP denotes Preparation
T2417 12-14 IN denotes of
T2418 15-23 NNP denotes Degraded
T2419 24-35 NNP denotes Carrageenan
T2420 36-39 CD denotes Two
T2421 40-52 NNS denotes preparations
T2422 53-55 IN denotes of
T2423 56-64 JJ denotes degraded
T2424 65-76 NN denotes carrageenan
T2425 77-81 IN denotes with
T2426 82-85 JJ denotes low
T2427 85-86 , denotes ,
T2428 87-88 -LRB- denotes (
T2429 88-89 FW denotes ∼
T2430 89-91 CD denotes 10
T2431 92-95 NN denotes kDa
T2432 95-96 : denotes ;
T2433 97-100 NNP denotes C10
T2434 100-101 -RRB- denotes )
T2435 101-102 , denotes ,
T2436 103-106 CC denotes and
T2437 107-113 NN denotes medium
T2438 113-114 , denotes ,
T2439 115-116 -LRB- denotes (
T2440 116-117 FW denotes ∼
T2441 117-119 CD denotes 40
T2442 120-123 NN denotes kDa
T2443 123-124 : denotes ;
T2444 125-128 NNP denotes C40
T2445 128-129 -RRB- denotes )
T2446 130-139 JJ denotes molecular
T2447 140-146 NN denotes weight
T2448 147-151 VBD denotes were
T2449 152-160 VBN denotes prepared
T2450 161-165 IN denotes from
T2451 166-172 JJ denotes native
T2452 173-189 JJ denotes iota-carrageenan
T2453 190-199 VBN denotes extracted
T2454 200-204 IN denotes from
T2455 205-212 NNP denotes Euchema
T2456 213-221 NN denotes spinosum
T2457 222-223 -LRB- denotes (
T2458 223-233 RB denotes generously
T2459 234-242 VBN denotes provided
T2460 243-245 IN denotes by
T2461 246-252 NNP denotes Sanofi
T2462 253-263 NNPS denotes Biosystems
T2463 264-272 NNP denotes Industry
T2464 272-273 , denotes ,
T2465 274-294 NNP denotes Boulogne-Billancourt
T2466 294-295 , denotes ,
T2467 296-302 NNP denotes France
T2468 302-303 -RRB- denotes )
T2469 303-304 . denotes .
T2470 305-311 JJ denotes Native
T2471 312-323 NN denotes carrageenan
T2472 324-327 VBD denotes was
T2473 328-337 VBN denotes dissolved
T2474 338-340 IN denotes in
T2475 341-350 JJ denotes distilled
T2476 351-356 NN denotes water
T2477 357-358 -LRB- denotes (
T2478 358-359 CD denotes 5
T2479 359-360 NN denotes %
T2480 361-364 NN denotes w/v
T2481 364-365 -RRB- denotes )
T2482 366-371 IN denotes under
T2483 372-380 JJ denotes vigorous
T2484 381-389 VBG denotes stirring
T2485 390-393 CC denotes and
T2486 394-400 VBN denotes heated
T2487 401-403 TO denotes to
T2488 404-406 CD denotes 60
T2489 406-407 CD denotes °
T2490 407-409 NNP denotes C.
T2491 410-414 RB denotes Then
T2492 414-415 , denotes ,
T2493 416-419 DT denotes the
T2494 420-431 NN denotes carrageenan
T2495 432-440 NN denotes solution
T2496 441-444 VBD denotes was
T2497 445-454 VBN denotes submitted
T2498 455-457 TO denotes to
T2499 458-461 CD denotes two
T2500 462-471 JJ denotes different
T2501 472-482 NNS denotes treatments
T2502 483-485 TO denotes to
T2503 486-492 VB denotes obtain
T2504 493-497 DT denotes both
T2505 498-501 JJ denotes low
T2506 502-505 CC denotes and
T2507 506-512 JJ denotes medium
T2508 513-522 JJ denotes molecular
T2509 523-529 NN denotes weight
T2510 530-539 NNS denotes fractions
T2511 539-540 . denotes .
T2512 541-548 RB denotes Briefly
T2513 548-549 , denotes ,
T2514 550-553 IN denotes for
T2515 554-557 DT denotes the
T2516 558-561 JJ denotes low
T2517 562-571 JJ denotes molecular
T2518 572-578 NN denotes weight
T2519 579-587 NN denotes fraction
T2520 587-588 , denotes ,
T2521 589-600 NN denotes carrageenan
T2522 601-609 NN denotes solution
T2523 610-613 VBD denotes was
T2524 614-624 VBN denotes hydrolyzed
T2525 625-629 IN denotes with
T2526 630-633 CD denotes 0.3
T2527 633-634 NN denotes %
T2528 635-636 -LRB- denotes (
T2529 636-639 FW denotes v/v
T2530 639-640 -RRB- denotes )
T2531 641-653 VBD denotes concentrated
T2532 654-663 JJ denotes sulphuric
T2533 664-668 NN denotes acid
T2534 669-672 IN denotes for
T2535 673-675 CD denotes 15
T2536 676-679 NN denotes min
T2537 680-682 IN denotes at
T2538 683-685 CD denotes 80
T2539 685-686 CD denotes °
T2540 686-687 NNP denotes C
T2541 687-688 . denotes .
T2542 689-694 IN denotes After
T2543 695-709 NN denotes neutralization
T2544 710-714 IN denotes with
T2545 715-719 NNP denotes NaOH
T2546 720-722 NNP denotes 4N
T2547 722-723 , denotes ,
T2548 724-727 DT denotes the
T2549 728-736 NN denotes solution
T2550 737-740 VBD denotes was
T2551 741-746 JJ denotes ultra
T2552 747-755 VBN denotes filtered
T2553 756-763 IN denotes through
T2554 764-765 DT denotes a
T2555 766-772 JJ denotes hollow
T2556 773-778 NN denotes fibre
T2557 779-788 NN denotes cartridge
T2558 789-793 IN denotes with
T2559 794-796 NNP denotes MW
T2560 797-804 NN denotes cut-off
T2561 805-806 CD denotes 5
T2562 807-810 NNP denotes kDa
T2563 810-811 , denotes ,
T2564 812-813 -LRB- denotes (
T2565 813-819 NNP denotes Amicon
T2566 820-823 NNP denotes Inc
T2567 823-824 , denotes ,
T2568 825-832 NNP denotes Beverly
T2569 832-833 , denotes ,
T2570 834-837 NNP denotes USA
T2571 837-838 -RRB- denotes )
T2572 838-839 . denotes .
T2573 840-843 IN denotes For
T2574 844-847 DT denotes the
T2575 848-854 NN denotes medium
T2576 855-864 JJ denotes molecular
T2577 865-871 NN denotes weight
T2578 872-880 NN denotes fraction
T2579 880-881 , denotes ,
T2580 882-885 DT denotes the
T2581 886-897 NN denotes carrageenan
T2582 898-906 NN denotes solution
T2583 907-910 VBD denotes was
T2584 911-921 VBN denotes hydrolyzed
T2585 922-926 IN denotes with
T2586 927-930 CD denotes 0.3
T2587 930-931 NN denotes %
T2588 932-933 -LRB- denotes (
T2589 933-936 FW denotes v/v
T2590 936-937 -RRB- denotes )
T2591 938-950 VBD denotes concentrated
T2592 951-960 JJ denotes sulphuric
T2593 961-965 NN denotes acid
T2594 966-969 IN denotes for
T2595 970-972 CD denotes 30
T2596 973-976 NN denotes min
T2597 977-979 IN denotes at
T2598 980-982 CD denotes 60
T2599 982-983 CD denotes °
T2600 983-984 NNP denotes C
T2601 984-985 . denotes .
T2602 986-991 IN denotes After
T2603 992-1006 NN denotes neutralization
T2604 1006-1007 , denotes ,
T2605 1008-1011 DT denotes the
T2606 1012-1023 NN denotes supernatant
T2607 1024-1027 VBD denotes was
T2608 1028-1033 JJ denotes ultra
T2609 1034-1042 VBN denotes filtered
T2610 1043-1044 -LRB- denotes (
T2611 1044-1046 NNP denotes MW
T2612 1047-1054 NN denotes cut-off
T2613 1055-1058 CD denotes 100
T2614 1059-1062 NNP denotes kDa
T2615 1062-1063 -RRB- denotes )
T2616 1063-1064 . denotes .
T2617 1065-1068 DT denotes The
T2618 1069-1077 NN denotes filtrate
T2619 1078-1081 VBD denotes was
T2620 1082-1091 VBN denotes submitted
T2621 1092-1094 TO denotes to
T2622 1095-1096 DT denotes a
T2623 1097-1103 JJ denotes second
T2624 1104-1109 NN denotes ultra
T2625 1110-1120 NN denotes filtration
T2626 1121-1122 -LRB- denotes (
T2627 1122-1124 NNP denotes MW
T2628 1125-1132 NN denotes cut-off
T2629 1133-1134 CD denotes 5
T2630 1135-1138 NNP denotes kDa
T2631 1138-1139 -RRB- denotes )
T2632 1139-1140 . denotes .
T2633 1141-1145 DT denotes Both
T2634 1146-1158 NNS denotes preparations
T2635 1159-1161 IN denotes of
T2636 1162-1166 NNP denotes dCGN
T2637 1167-1171 VBD denotes were
T2638 1172-1184 VBN denotes precipitated
T2639 1185-1189 IN denotes with
T2640 1190-1191 CD denotes 4
T2641 1192-1199 NNS denotes volumes
T2642 1200-1202 IN denotes of
T2643 1203-1205 CD denotes 95
T2644 1205-1206 NN denotes %
T2645 1207-1214 NN denotes ethanol
T2646 1214-1215 , denotes ,
T2647 1216-1221 VBD denotes dried
T2648 1222-1224 IN denotes at
T2649 1225-1229 NN denotes room
T2650 1230-1241 NN denotes temperature
T2651 1242-1245 CC denotes and
T2652 1246-1252 NN denotes ground
T2653 1253-1255 TO denotes to
T2654 1256-1261 JJ denotes small
T2655 1262-1271 NNS denotes particles
T2656 1272-1273 -LRB- denotes (
T2657 1273-1274 CD denotes 1
T2658 1275-1277 NN denotes mm
T2659 1278-1280 IN denotes in
T2660 1281-1289 NN denotes diameter
T2661 1289-1290 -RRB- denotes )
T2662 1290-1291 . denotes .
T2663 1292-1297 VBG denotes Using
T2664 1298-1312 NN denotes gel-permeation
T2665 1313-1327 NN denotes chromatography
T2666 1328-1330 IN denotes in
T2667 1331-1342 NN denotes combination
T2668 1343-1347 IN denotes with
T2669 1348-1353 JJ denotes light
T2670 1354-1364 VBG denotes scattering
T2671 1365-1377 NNS denotes measurements
T2672 1378-1379 -LRB- denotes (
T2673 1379-1382 VB denotes see
T2674 1383-1389 NNP denotes Viebke
T2675 1390-1392 FW denotes et
T2676 1393-1396 FW denotes al.
T2677 1397-1398 NNP denotes [
T2678 1398-1400 CD denotes 21
T2679 1400-1401 NNP denotes ]
T2680 1401-1402 -RRB- denotes )
T2681 1402-1403 , denotes ,
T2682 1404-1406 PRP denotes it
T2683 1407-1410 VBD denotes was
T2684 1411-1420 VBN denotes confirmed
T2685 1421-1425 IN denotes that
T2686 1426-1429 DT denotes the
T2687 1430-1433 JJ denotes low
T2688 1434-1442 NN denotes fraction
T2689 1443-1446 VBD denotes had
T2690 1447-1449 DT denotes an
T2691 1450-1457 JJ denotes average
T2692 1458-1467 JJ denotes molecular
T2693 1468-1474 NN denotes weight
T2694 1475-1477 IN denotes of
T2695 1478-1480 CD denotes 10
T2696 1481-1484 NN denotes kDa
T2697 1484-1485 , denotes ,
T2698 1486-1489 CC denotes and
T2699 1490-1493 DT denotes the
T2700 1494-1500 NN denotes medium
T2701 1501-1509 NN denotes fraction
T2702 1510-1512 IN denotes of
T2703 1513-1515 CD denotes 40
T2704 1516-1519 NN denotes kDa
T2705 1519-1520 . denotes .
T2706 1521-1524 DT denotes The
T2707 1525-1533 NN denotes sulphate
T2708 1534-1541 NN denotes content
T2709 1542-1544 IN denotes of
T2710 1545-1560 NNS denotes polysaccharides
T2711 1561-1563 IN denotes in
T2712 1564-1568 DT denotes both
T2713 1569-1578 NNS denotes fractions
T2714 1579-1582 VBD denotes was
T2715 1583-1591 VBN denotes measured
T2716 1592-1601 VBG denotes following
T2717 1602-1605 DT denotes the
T2718 1606-1612 NN denotes method
T2719 1613-1615 IN denotes of
T2720 1616-1624 NNP denotes Quemener
T2721 1625-1627 FW denotes et
T2722 1628-1631 FW denotes al.
T2723 1632-1633 NNP denotes [
T2724 1633-1635 CD denotes 22
T2725 1635-1636 NNP denotes ]
T2726 1636-1637 . denotes .
T2727 1638-1645 RB denotes Finally
T2728 1645-1646 , denotes ,
T2729 1647-1650 DT denotes the
T2730 1651-1658 NN denotes absence
T2731 1659-1661 IN denotes of
T2732 1662-1676 NN denotes polysaccharide
T2733 1677-1686 NN denotes structure
T2734 1687-1700 NNS denotes modifications
T2735 1701-1703 IN denotes in
T2736 1704-1707 DT denotes the
T2737 1708-1711 CD denotes two
T2738 1712-1721 NNS denotes fractions
T2739 1722-1725 VBD denotes was
T2740 1726-1735 VBN denotes confirmed
T2741 1736-1741 VBG denotes using
T2742 1742-1748 JJ denotes 2H-NMR
T2743 1749-1761 NN denotes spectroscopy
T2744 1761-1762 . denotes .
T2745 1763-1766 DT denotes The
T2746 1767-1774 NN denotes absence
T2747 1775-1777 IN denotes of
T2748 1778-1781 NNP denotes LPS
T2749 1782-1795 NN denotes contamination
T2750 1796-1798 IN denotes in
T2751 1799-1802 DT denotes the
T2752 1803-1806 CD denotes two
T2753 1807-1816 NNS denotes fractions
T2754 1817-1820 VBD denotes was
T2755 1821-1830 VBN denotes confirmed
T2756 1831-1836 VBG denotes using
T2757 1837-1840 DT denotes the
T2758 1841-1849 JJ denotes e-Toxate
T2759 1849-1850 NN denotes ®
T2760 1851-1854 NN denotes kit
T2761 1855-1856 -LRB- denotes (
T2762 1856-1861 NNP denotes Sigma
T2763 1861-1862 , denotes ,
T2764 1863-1865 NNP denotes St
T2765 1866-1873 NNP denotes Quentin
T2766 1874-1883 NNP denotes Fallavier
T2767 1883-1884 , denotes ,
T2768 1885-1891 NNP denotes France
T2769 1891-1892 -RRB- denotes )
T2770 1892-1893 . denotes .
T2771 1894-1900 IN denotes Before
T2772 1901-1904 NN denotes use
T2773 1905-1907 IN denotes in
T2774 1908-1912 NN denotes cell
T2775 1913-1920 NN denotes culture
T2776 1920-1921 , denotes ,
T2777 1922-1925 DT denotes the
T2778 1926-1929 CD denotes two
T2779 1930-1939 NNS denotes fractions
T2780 1940-1944 VBD denotes were
T2781 1945-1954 VBN denotes dissolved
T2782 1955-1957 IN denotes in
T2783 1958-1966 JJ denotes complete
T2784 1967-1973 NN denotes medium
T2785 1974-1980 IN denotes during
T2786 1981-1983 CD denotes 30
T2787 1984-1987 NN denotes min
T2788 1988-1990 IN denotes at
T2789 1991-1993 CD denotes 56
T2790 1993-1994 CD denotes °
T2791 1994-1996 NNP denotes C.
R2132 T2416 T2449 nsubjpass Preparation,prepared
R2133 T2417 T2416 prep of,Preparation
R2134 T2418 T2419 compound Degraded,Carrageenan
R2135 T2419 T2417 pobj Carrageenan,of
R2136 T2420 T2421 nummod Two,preparations
R2137 T2421 T2447 nmod preparations,weight
R2138 T2422 T2421 prep of,preparations
R2139 T2423 T2424 amod degraded,carrageenan
R2140 T2424 T2422 pobj carrageenan,of
R2141 T2425 T2424 prep with,carrageenan
R2142 T2426 T2425 pobj low,with
R2143 T2427 T2421 punct ",",preparations
R2144 T2428 T2429 punct (,∼
R2145 T2429 T2421 appos ∼,preparations
R2146 T2430 T2431 nummod 10,kDa
R2147 T2431 T2429 npadvmod kDa,∼
R2148 T2432 T2421 punct ;,preparations
R2149 T2433 T2421 appos C10,preparations
R2150 T2434 T2421 punct ),preparations
R2151 T2435 T2421 punct ",",preparations
R2152 T2436 T2421 cc and,preparations
R2180 T2464 T2463 punct ",",Industry
R2181 T2465 T2463 conj Boulogne-Billancourt,Industry
R2182 T2466 T2465 punct ",",Boulogne-Billancourt
R2183 T2467 T2465 conj France,Boulogne-Billancourt
R2184 T2468 T2452 punct ),iota-carrageenan
R2185 T2469 T2449 punct .,prepared
R2186 T2470 T2471 amod Native,carrageenan
R2187 T2471 T2473 nsubjpass carrageenan,dissolved
R2188 T2472 T2473 auxpass was,dissolved
R2189 T2473 T2473 ROOT dissolved,dissolved
R2190 T2474 T2473 prep in,dissolved
R2191 T2475 T2476 amod distilled,water
R2192 T2476 T2474 pobj water,in
R2193 T2477 T2480 punct (,w/v
R2194 T2478 T2479 nummod 5,%
R2195 T2479 T2480 compound %,w/v
R2196 T2480 T2473 parataxis w/v,dissolved
R2197 T2481 T2480 punct ),w/v
R2198 T2482 T2473 prep under,dissolved
R2199 T2483 T2484 amod vigorous,stirring
R2200 T2484 T2482 pobj stirring,under
R2201 T2485 T2484 cc and,stirring
R2202 T2486 T2484 conj heated,stirring
R2203 T2487 T2486 prep to,heated
R2204 T2488 T2489 nummod 60,°
R2232 T2516 T2518 amod low,weight
R2233 T2517 T2518 amod molecular,weight
R2234 T2518 T2519 compound weight,fraction
R2235 T2519 T2514 pobj fraction,for
R2236 T2520 T2524 punct ",",hydrolyzed
R2237 T2521 T2522 compound carrageenan,solution
R2238 T2522 T2524 nsubjpass solution,hydrolyzed
R2239 T2523 T2524 auxpass was,hydrolyzed
R2240 T2524 T2524 ROOT hydrolyzed,hydrolyzed
R2241 T2525 T2524 prep with,hydrolyzed
R2242 T2526 T2527 nummod 0.3,%
R2243 T2527 T2525 pobj %,with
R2244 T2528 T2529 punct (,v/v
R2245 T2529 T2527 appos v/v,%
R2246 T2530 T2524 punct ),hydrolyzed
R2247 T2531 T2533 nmod concentrated,acid
R2248 T2532 T2533 compound sulphuric,acid
R2249 T2533 T2524 dobj acid,hydrolyzed
R2250 T2534 T2524 prep for,hydrolyzed
R2251 T2535 T2536 nummod 15,min
R2252 T2536 T2534 pobj min,for
R2253 T2537 T2524 prep at,hydrolyzed
R2254 T2538 T2540 nummod 80,C
R2255 T2539 T2540 nummod °,C
R2256 T2540 T2537 pobj C,at
R2257 T2541 T2524 punct .,hydrolyzed
R2258 T2542 T2552 prep After,filtered
R2259 T2543 T2542 pobj neutralization,After
R2260 T2544 T2543 prep with,neutralization
R2261 T2545 T2546 compound NaOH,4N
R2262 T2546 T2544 pobj 4N,with
R2263 T2547 T2552 punct ",",filtered
R2264 T2548 T2549 det the,solution
R2265 T2549 T2552 nsubjpass solution,filtered
R2266 T2550 T2552 auxpass was,filtered
R2267 T2551 T2552 advmod ultra,filtered
R2268 T2552 T2552 ROOT filtered,filtered
R2269 T2553 T2552 prep through,filtered
R2270 T2554 T2557 det a,cartridge
R2271 T2555 T2557 amod hollow,cartridge
R2272 T2556 T2557 compound fibre,cartridge
R2273 T2557 T2553 pobj cartridge,through
R2274 T2558 T2557 prep with,cartridge
R2275 T2559 T2562 nmod MW,kDa
R2276 T2560 T2562 nmod cut-off,kDa
R2277 T2561 T2562 nummod 5,kDa
R2278 T2562 T2558 pobj kDa,with
R2279 T2563 T2562 punct ",",kDa
R2280 T2564 T2566 punct (,Inc
R2281 T2565 T2566 compound Amicon,Inc
R2282 T2566 T2562 conj Inc,kDa
R2283 T2567 T2566 punct ",",Inc
R2284 T2568 T2566 conj Beverly,Inc
R2285 T2569 T2568 punct ",",Beverly
R2286 T2570 T2568 conj USA,Beverly
R2287 T2571 T2566 punct ),Inc
R2288 T2572 T2552 punct .,filtered
R2289 T2573 T2584 prep For,hydrolyzed
R2290 T2574 T2578 det the,fraction
R2291 T2575 T2578 compound medium,fraction
R2292 T2576 T2577 amod molecular,weight
R2293 T2577 T2578 compound weight,fraction
R2294 T2578 T2573 pobj fraction,For
R2295 T2579 T2584 punct ",",hydrolyzed
R2296 T2580 T2582 det the,solution
R2297 T2581 T2582 compound carrageenan,solution
R2298 T2582 T2584 nsubjpass solution,hydrolyzed
R2299 T2583 T2584 auxpass was,hydrolyzed
R2300 T2584 T2584 ROOT hydrolyzed,hydrolyzed
R2301 T2585 T2584 prep with,hydrolyzed
R2302 T2586 T2587 nummod 0.3,%
R2303 T2587 T2585 pobj %,with
R2304 T2588 T2589 punct (,v/v
R2305 T2589 T2587 appos v/v,%
R2306 T2590 T2584 punct ),hydrolyzed
R2307 T2591 T2591 ROOT concentrated,concentrated
R2308 T2592 T2593 amod sulphuric,acid
R2309 T2593 T2591 dobj acid,concentrated
R2310 T2594 T2591 prep for,concentrated
R2311 T2595 T2596 nummod 30,min
R2312 T2596 T2594 pobj min,for
R2313 T2597 T2591 prep at,concentrated
R2314 T2598 T2599 nummod 60,°
R2315 T2599 T2597 pobj °,at
R2316 T2600 T2591 npadvmod C,concentrated
R2317 T2601 T2584 punct .,hydrolyzed
R2318 T2602 T2609 prep After,filtered
R2319 T2603 T2602 pobj neutralization,After
R2320 T2604 T2609 punct ",",filtered
R2321 T2605 T2606 det the,supernatant
R2322 T2606 T2609 nsubjpass supernatant,filtered
R2323 T2607 T2609 auxpass was,filtered
R2324 T2608 T2609 advmod ultra,filtered
R2325 T2609 T2609 ROOT filtered,filtered
R2326 T2610 T2614 punct (,kDa
R2327 T2611 T2614 prt MW,kDa
R2328 T2612 T2614 nmod cut-off,kDa
R2329 T2613 T2614 nummod 100,kDa
R2330 T2614 T2609 dobj kDa,filtered
R2331 T2615 T2614 punct ),kDa
R2332 T2616 T2609 punct .,filtered
R2333 T2617 T2618 det The,filtrate
R2334 T2618 T2620 nsubjpass filtrate,submitted
R2335 T2619 T2620 auxpass was,submitted
R2336 T2620 T2620 ROOT submitted,submitted
R2337 T2621 T2620 prep to,submitted
R2338 T2622 T2625 det a,filtration
R2339 T2623 T2625 amod second,filtration
R2340 T2624 T2625 compound ultra,filtration
R2341 T2625 T2621 pobj filtration,to
R2342 T2626 T2630 punct (,kDa
R2343 T2627 T2630 nmod MW,kDa
R2344 T2628 T2630 punct cut-off,kDa
R2345 T2629 T2628 nummod 5,cut-off
R2346 T2630 T2625 appos kDa,filtration
R2347 T2631 T2630 punct ),kDa
R2348 T2632 T2620 punct .,submitted
R2349 T2633 T2634 det Both,preparations
R2350 T2634 T2638 nsubjpass preparations,precipitated
R2351 T2635 T2634 prep of,preparations
R2352 T2636 T2635 pobj dCGN,of
R2353 T2637 T2638 auxpass were,precipitated
R2354 T2638 T2638 ROOT precipitated,precipitated
R2355 T2639 T2638 prep with,precipitated
R2356 T2640 T2641 nummod 4,volumes
R2357 T2641 T2639 pobj volumes,with
R2358 T2642 T2641 prep of,volumes
R2359 T2643 T2644 nummod 95,%
R2360 T2644 T2645 compound %,ethanol
R2361 T2645 T2642 pobj ethanol,of
R2362 T2646 T2638 punct ",",precipitated
R2363 T2647 T2638 conj dried,precipitated
R2364 T2648 T2647 prep at,dried
R2365 T2649 T2650 compound room,temperature
R2366 T2650 T2648 pobj temperature,at
R2367 T2651 T2650 cc and,temperature
R2368 T2652 T2650 conj ground,temperature
R2369 T2653 T2647 prep to,dried
R2370 T2654 T2655 amod small,particles
R2371 T2655 T2653 pobj particles,to
R2372 T2656 T2658 punct (,mm
R2373 T2657 T2658 nummod 1,mm
R2374 T2658 T2655 parataxis mm,particles
R2375 T2659 T2658 prep in,mm
R2376 T2660 T2659 pobj diameter,in
R2377 T2661 T2658 punct ),mm
R2378 T2662 T2638 punct .,precipitated
R2379 T2663 T2663 ROOT Using,Using
R2380 T2664 T2665 compound gel-permeation,chromatography
R2381 T2665 T2663 dobj chromatography,Using
R2382 T2666 T2665 prep in,chromatography
R2383 T2667 T2666 pobj combination,in
R2384 T2668 T2663 prep with,Using
R2385 T2669 T2671 amod light,measurements
R2386 T2670 T2671 compound scattering,measurements
R2387 T2671 T2668 pobj measurements,with
R2388 T2672 T2673 punct (,see
R2389 T2673 T2663 parataxis see,Using
R2390 T2674 T2684 nsubj Viebke,confirmed
R2391 T2675 T2676 compound et,al.
R2392 T2676 T2684 prep al.,confirmed
R2393 T2677 T2676 appos [,al.
R2394 T2678 T2679 nummod 21,]
R2395 T2679 T2677 appos ],[
R2396 T2680 T2677 punct ),[
R2397 T2681 T2684 punct ",",confirmed
R2398 T2682 T2684 nsubjpass it,confirmed
R2399 T2683 T2684 auxpass was,confirmed
R2400 T2684 T2673 ccomp confirmed,see
R2401 T2685 T2689 mark that,had
R2402 T2686 T2688 det the,fraction
R2403 T2687 T2688 amod low,fraction
R2404 T2688 T2689 nsubj fraction,had
R2405 T2689 T2684 ccomp had,confirmed
R2406 T2690 T2693 det an,weight
R2407 T2691 T2693 amod average,weight
R2408 T2692 T2693 amod molecular,weight
R2409 T2693 T2689 dobj weight,had
R2410 T2694 T2693 prep of,weight
R2411 T2695 T2696 nummod 10,kDa
R2412 T2696 T2694 pobj kDa,of
R2413 T2697 T2689 punct ",",had
R2414 T2698 T2689 cc and,had
R2415 T2699 T2701 det the,fraction
R2416 T2700 T2701 compound medium,fraction
R2417 T2701 T2689 conj fraction,had
R2418 T2702 T2701 prep of,fraction
R2419 T2703 T2704 nummod 40,kDa
R2420 T2704 T2702 pobj kDa,of
R2421 T2705 T2673 punct .,see
R2422 T2706 T2708 det The,content
R2423 T2707 T2708 compound sulphate,content
R2424 T2708 T2715 nsubjpass content,measured
R2425 T2709 T2708 prep of,content
R2426 T2710 T2709 pobj polysaccharides,of
R2427 T2711 T2710 prep in,polysaccharides
R2428 T2712 T2713 det both,fractions
R2429 T2713 T2711 pobj fractions,in
R2430 T2714 T2715 auxpass was,measured
R2431 T2715 T2715 ROOT measured,measured
R2432 T2716 T2715 prep following,measured
R2433 T2717 T2718 det the,method
R2434 T2718 T2716 pobj method,following
R2435 T2719 T2718 prep of,method
R2436 T2720 T2723 compound Quemener,[
R2437 T2721 T2722 compound et,al.
R2438 T2722 T2723 compound al.,[
R2439 T2723 T2719 pobj [,of
R2440 T2724 T2725 nummod 22,]
R2441 T2725 T2718 npadvmod ],method
R2442 T2726 T2715 punct .,measured
R2443 T2727 T2740 advmod Finally,confirmed
R2444 T2728 T2740 punct ",",confirmed
R2445 T2729 T2730 det the,absence
R2446 T2730 T2740 nsubjpass absence,confirmed
R2447 T2731 T2730 prep of,absence
R2448 T2732 T2733 compound polysaccharide,structure
R2449 T2733 T2734 compound structure,modifications
R2450 T2734 T2731 pobj modifications,of
R2451 T2735 T2734 prep in,modifications
R2452 T2736 T2738 det the,fractions
R2453 T2737 T2738 nummod two,fractions
R2454 T2738 T2735 pobj fractions,in
R2455 T2739 T2740 auxpass was,confirmed
R2456 T2740 T2740 ROOT confirmed,confirmed
R2457 T2741 T2740 advcl using,confirmed
R2458 T2742 T2743 amod 2H-NMR,spectroscopy
R2459 T2743 T2741 dobj spectroscopy,using
R2460 T2744 T2740 punct .,confirmed
R2461 T2745 T2746 det The,absence
R2462 T2746 T2755 nsubjpass absence,confirmed
R2463 T2747 T2746 prep of,absence
R2464 T2748 T2749 compound LPS,contamination
R2465 T2749 T2747 pobj contamination,of
R2466 T2750 T2749 prep in,contamination
R2467 T2751 T2753 det the,fractions
R2468 T2752 T2753 nummod two,fractions
R2469 T2753 T2750 pobj fractions,in
R2470 T2754 T2755 auxpass was,confirmed
R2471 T2755 T2755 ROOT confirmed,confirmed
R2472 T2756 T2755 advcl using,confirmed
R2473 T2757 T2760 det the,kit
R2474 T2758 T2760 amod e-Toxate,kit
R2475 T2759 T2760 compound ®,kit
R2476 T2760 T2756 dobj kit,using
R2477 T2761 T2760 punct (,kit
R2478 T2762 T2760 appos Sigma,kit
R2479 T2763 T2762 punct ",",Sigma
R2480 T2764 T2766 compound St,Fallavier
R2481 T2765 T2766 compound Quentin,Fallavier
R2482 T2766 T2762 conj Fallavier,Sigma
R2483 T2767 T2766 punct ",",Fallavier
R2484 T2768 T2766 conj France,Fallavier
R2485 T2769 T2766 punct ),Fallavier
R2486 T2770 T2755 punct .,confirmed
R2487 T2771 T2781 prep Before,dissolved
R2488 T2772 T2771 pobj use,Before
R2489 T2773 T2772 prep in,use
R2490 T2774 T2775 compound cell,culture
R2491 T2775 T2773 pobj culture,in
R2492 T2776 T2781 punct ",",dissolved
R2153 T2437 T2421 conj medium,preparations
R2154 T2438 T2437 punct ",",medium
R2155 T2439 T2437 punct (,medium
R2156 T2440 T2437 appos ∼,medium
R2157 T2441 T2442 nummod 40,kDa
R2158 T2442 T2440 appos kDa,∼
R2159 T2443 T2437 punct ;,medium
R2160 T2444 T2421 appos C40,preparations
R2161 T2445 T2421 punct ),preparations
R2162 T2446 T2447 amod molecular,weight
R2163 T2447 T2416 conj weight,Preparation
R2164 T2448 T2449 auxpass were,prepared
R2165 T2449 T2449 ROOT prepared,prepared
R2166 T2450 T2449 prep from,prepared
R2167 T2451 T2452 amod native,iota-carrageenan
R2168 T2452 T2450 pobj iota-carrageenan,from
R2169 T2453 T2452 acl extracted,iota-carrageenan
R2170 T2454 T2453 prep from,extracted
R2171 T2455 T2456 compound Euchema,spinosum
R2172 T2456 T2454 pobj spinosum,from
R2173 T2457 T2459 punct (,provided
R2174 T2458 T2459 advmod generously,provided
R2175 T2459 T2452 acl provided,iota-carrageenan
R2176 T2460 T2459 agent by,provided
R2177 T2461 T2463 compound Sanofi,Industry
R2178 T2462 T2463 compound Biosystems,Industry
R2179 T2463 T2460 pobj Industry,by
R2205 T2489 T2487 pobj °,to
R2206 T2490 T2491 compound C.,Then
R2207 T2491 T2497 advmod Then,submitted
R2208 T2492 T2497 punct ",",submitted
R2209 T2493 T2495 det the,solution
R2210 T2494 T2495 compound carrageenan,solution
R2211 T2495 T2497 nsubjpass solution,submitted
R2212 T2496 T2497 auxpass was,submitted
R2213 T2497 T2497 ROOT submitted,submitted
R2214 T2498 T2497 prep to,submitted
R2215 T2499 T2501 nummod two,treatments
R2216 T2500 T2501 amod different,treatments
R2217 T2501 T2498 pobj treatments,to
R2218 T2502 T2503 aux to,obtain
R2219 T2503 T2497 xcomp obtain,submitted
R2220 T2504 T2505 advmod both,low
R2221 T2505 T2510 amod low,fractions
R2222 T2506 T2505 cc and,low
R2223 T2507 T2505 conj medium,low
R2224 T2508 T2509 amod molecular,weight
R2225 T2509 T2510 compound weight,fractions
R2226 T2510 T2503 dobj fractions,obtain
R2227 T2511 T2497 punct .,submitted
R2228 T2512 T2524 advmod Briefly,hydrolyzed
R2229 T2513 T2524 punct ",",hydrolyzed
R2230 T2514 T2524 prep for,hydrolyzed
R2231 T2515 T2519 det the,fraction
R2493 T2777 T2779 det the,fractions
R2494 T2778 T2779 nummod two,fractions
R2495 T2779 T2781 nsubjpass fractions,dissolved
R2496 T2780 T2781 auxpass were,dissolved
R2497 T2781 T2781 ROOT dissolved,dissolved
R2498 T2782 T2781 prep in,dissolved
R2499 T2783 T2784 amod complete,medium
R2500 T2784 T2782 pobj medium,in
R2501 T2785 T2781 prep during,dissolved
R2502 T2786 T2787 nummod 30,min
R2503 T2787 T2785 pobj min,during
R2504 T2788 T2787 prep at,min
R2505 T2789 T2790 nummod 56,°
R2506 T2790 T2791 nummod °,C.
R2507 T2791 T2788 pobj C.,at

sentences

Id Subject Object Predicate Lexical cue
T2051 0-35 Sentence denotes Preparation of Degraded Carrageenan
T2052 36-304 Sentence denotes Two preparations of degraded carrageenan with low, (∼10 kDa; C10), and medium, (∼40 kDa; C40) molecular weight were prepared from native iota-carrageenan extracted from Euchema spinosum (generously provided by Sanofi Biosystems Industry, Boulogne-Billancourt, France).
T2053 305-540 Sentence denotes Native carrageenan was dissolved in distilled water (5% w/v) under vigorous stirring and heated to 60°C. Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions.
T2054 541-839 Sentence denotes Briefly, for the low molecular weight fraction, carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 15 min at 80°C. After neutralization with NaOH 4N, the solution was ultra filtered through a hollow fibre cartridge with MW cut-off 5 kDa, (Amicon Inc, Beverly, USA).
T2055 840-1064 Sentence denotes For the medium molecular weight fraction, the carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 30 min at 60°C. After neutralization, the supernatant was ultra filtered (MW cut-off 100 kDa).
T2056 1065-1140 Sentence denotes The filtrate was submitted to a second ultra filtration (MW cut-off 5 kDa).
T2057 1141-1291 Sentence denotes Both preparations of dCGN were precipitated with 4 volumes of 95% ethanol, dried at room temperature and ground to small particles (1 mm in diameter).
T2058 1292-1520 Sentence denotes Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa.
T2059 1521-1637 Sentence denotes The sulphate content of polysaccharides in both fractions was measured following the method of Quemener et al. [22].
T2060 1638-1762 Sentence denotes Finally, the absence of polysaccharide structure modifications in the two fractions was confirmed using 2H-NMR spectroscopy.
T2061 1763-1893 Sentence denotes The absence of LPS contamination in the two fractions was confirmed using the e-Toxate® kit (Sigma, St Quentin Fallavier, France).
T2062 1894-1996 Sentence denotes Before use in cell culture, the two fractions were dissolved in complete medium during 30 min at 56°C.
T40 0-35 Sentence denotes Preparation of Degraded Carrageenan
T41 36-304 Sentence denotes Two preparations of degraded carrageenan with low, (∼10 kDa; C10), and medium, (∼40 kDa; C40) molecular weight were prepared from native iota-carrageenan extracted from Euchema spinosum (generously provided by Sanofi Biosystems Industry, Boulogne-Billancourt, France).
T42 305-409 Sentence denotes Native carrageenan was dissolved in distilled water (5% w/v) under vigorous stirring and heated to 60°C.
T43 410-540 Sentence denotes Then, the carrageenan solution was submitted to two different treatments to obtain both low and medium molecular weight fractions.
T44 541-688 Sentence denotes Briefly, for the low molecular weight fraction, carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 15 min at 80°C.
T45 689-839 Sentence denotes After neutralization with NaOH 4N, the solution was ultra filtered through a hollow fibre cartridge with MW cut-off 5 kDa, (Amicon Inc, Beverly, USA).
T46 840-985 Sentence denotes For the medium molecular weight fraction, the carrageenan solution was hydrolyzed with 0.3% (v/v) concentrated sulphuric acid for 30 min at 60°C.
T47 986-1064 Sentence denotes After neutralization, the supernatant was ultra filtered (MW cut-off 100 kDa).
T48 1065-1140 Sentence denotes The filtrate was submitted to a second ultra filtration (MW cut-off 5 kDa).
T49 1141-1291 Sentence denotes Both preparations of dCGN were precipitated with 4 volumes of 95% ethanol, dried at room temperature and ground to small particles (1 mm in diameter).
T50 1292-1520 Sentence denotes Using gel-permeation chromatography in combination with light scattering measurements (see Viebke et al. [21]), it was confirmed that the low fraction had an average molecular weight of 10 kDa, and the medium fraction of 40 kDa.
T51 1521-1637 Sentence denotes The sulphate content of polysaccharides in both fractions was measured following the method of Quemener et al. [22].
T52 1638-1762 Sentence denotes Finally, the absence of polysaccharide structure modifications in the two fractions was confirmed using 2H-NMR spectroscopy.
T53 1763-1893 Sentence denotes The absence of LPS contamination in the two fractions was confirmed using the e-Toxate® kit (Sigma, St Quentin Fallavier, France).
T54 1894-1996 Sentence denotes Before use in cell culture, the two fractions were dissolved in complete medium during 30 min at 56°C.

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T2808 1856-1861 Protein denotes Sigma
T2809 1863-1873 Protein denotes St Quentin