PMC:2791889 / 33383-33910
Annnotations
bionlp-st-ge-2016-uniprot
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bionlp-st-ge-2016-reference
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2_test
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pmc-enju-pas
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bionlp-st-ge-2016-spacy-parsed
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Detection by ICS and ELISA\nAt day 5 or 7, cells were restimulated with immobilized anti-CD3 (2 μg/ml) and anti-CD28 (2 μg/ml) (4 hr with BrefeldinA [10 μg/ml] in the last 2 hr) or with PdBU and Ionomycin (5 hr with BrefeldinA [1 μg/ml]). After ICS FACS, data were collected on a FACSCalibur (Becton Dickinson) and analyzed with FlowJo (Tree Star). For ELISA, cells were similarly restimulated for 48 hr and supernatant was collected and analyzed for IL-4, IL-10, and IFN-γ as described before (Shoemaker et al., 2006)."}
GO-MF
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GO-CC
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sentences
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events-check-again
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bionlp-st-ge-2016-reference-tees
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test2
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