PMC:2724026 / 41120-42841 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2724026","sourcedb":"PMC","sourceid":"2724026","source_url":"https://www.ncbi.nlm.nih.gov/pmc/2724026","text":"Table 3 Folding data for FADD DD helix scanning mutants\nFADD DD variant ΔGD-N (kcal mol-1) ΔΔGD-N (kcal mol-1) mD-N (kcal mol-1 M-1) kf2 M (s-1) Φ2 M\nHelix 1\nCys98Ala 7.2 1.8 48\nCys98Gly 6.2 1.0 1.6 26 0.38\nAsn102Ala 6.9 1.3 58\nAsn102Gly 6.3 0.7 1.3 33 0.50\nHelix2\nArg113Ala 6.5 1.2 52\nArg113Gly 5.4 1.1 1.1 38 0.17\nArg114Ala 6.4 1.6 46\nArg114Gly 5.0 1.4 1.6 28 0.20\nArg117Ala 6.3 1.2 47\nArg117Gly 5.2 1.1 1.1 31 0.23\nHelix3\nAsp127Ala 7.2 1.5 49\nAsp127Gly 6.2 1.0 1.4 45 0.07\nSer128Ala 7.0 1.1 37\nSer128Gly 6.6 0.43 1.4 47 n.d.\nAsp131Ala 7.5 1.4 44\nAsp131Gly 6.5 1.0 1.4 46 –0.03\nArg132Ala 6.6 1.2 40\nArg132Gly 5.5 1.1 1.1 43 –0.04\nHelix4\nThr138Ala 7.1 1.4 68\nThr138Gly 6.4 0.7 1.5 35 0.56\nGlu139Ala 6.4 1.4 29\nGlu139Gly 5.7 0.7 1.3 16 0.49\nArg142Ala 5.9 1.3 32\nArg142Gly 4.8 1.1 1.3 12 0.51\nGlu143Ala 6.6 1.4 34\nGlu143Gly 4.2 2.4 1.7 10 0.29\nArg146Ala 6.3 30\nArg146Gly 5.1 1.2 20 20 0.19\nAsn150Ala 6.8 2.0 46\nAsn150Gly 6.2 0.6 1.5 32 0.35\nHelix5\nArg166Ala 4.6 1.3 22\nArg166Gly 3.7 0.9 1.6 13 0.30\nSer167Ala 7.6 1.7 69\nSer167Gly 6.4 1.3 1.7 28 0.42\nHelix6\nAsp175Ala 6.4 1.4 38\nAsp175Gly 5.4 1.0 1.5 23 0.12\nGln178Ala 6.6 1.2 49\nGln178Gly 5.6 1.0 1.4 27 0.31\nThe ΔΔGD-N and Φ-values are determined for the Ala to Gly mutation. For clarity, errors are not shown in the table. The error in free energy measurements is in the range ± 0.1–0.2 kcal mol-1; the error in kf2M is ± 10% and the error in Φ is \u003c 0.1. Note that Φ-values cannot be considered reliable if ΔΔGD-N is low,27 so Φ-values were not determined when ΔΔGD-N \u003c 0.6 kcal mol- 1 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