PMC:2724026 / 12572-13304 JSONTXT

{"project":"2_test","denotations":[{"id":"19362094-1569556-62520522","span":{"begin":463,"end":465},"obj":"1569556"},{"id":"19362094-18538343-62520523","span":{"begin":639,"end":641},"obj":"18538343"}],"text":"Φ-Values obtained were either zero or fractional, with no Φ-value of 1 (Tables 2 and 3), indicating no part of the protein is as fully formed in the transition state as it is in the native state. To interpret a Φ-value analysis, it is customary to consider Φ-value patterns, rather than to try to interpret individual Φ-values. This allows one to determine which regions of the protein are fully unfolded, partially folded or fully folded in the transition state.28 The Φ-values obtained are thus generally classified into low, medium and high classes, with the boundaries chosen to reflect the overall Φ-value distribution (e.g. see Ref. 29): in our case, the cut-offs used are: low, Φ ≤ 0.15; medium, Φ = 0.16–0.5; high, Φ ≥ 0.51."}