PMC:2724026 / 10979-11941 JSONTXT

{"project":"2_test","denotations":[{"id":"19362094-8356089-62520520","span":{"begin":511,"end":513},"obj":"8356089"}],"text":"Some mutant chevron plots had linear unfolding limbs, as was seen in WT. However, many mutants exhibited some downward curvature in the unfolding arm of their chevron plot and for a few mutants this curvature was very significant (Fig. 2). (What curved chevron plots may mean is discussed later). It was therefore not possible to do any global fitting of the data. All chevrons were fit individually: first, the chevrons were fit to an equation with a quadratic term in the unfolding limb only (a “Hammond” fit,26 see Eq. (3)); second, each chevron plot was fit individually to an equation describing a linear dependence of both lnkf and lnku on the denaturant concentration (linear chevron fit, see Eq. (2)). To fit the kinetic data to the linear chevron fit, data points that were judged to be curving downwards were omitted from the fit. (All chevrons are shown in Supplementary Data Figs. S1 and S2, fit to a linear equation, identifying the points omitted.)"}