PMC:2674207 / 455-2163 JSON TXT

Annnotations TAB JSON ListView MergeView

pmc-enju-pas

bionlp-st-ge-2016-coref

{"project":"bionlp-st-ge-2016-coref","denotations":[{"id":"T426","span":{"begin":72,"end":85},"obj":"Anaphor"},{"id":"T427","span":{"begin":86,"end":104},"obj":"Antecedent"},{"id":"T428","span":{"begin":106,"end":110},"obj":"Antecedent"},{"id":"T429","span":{"begin":116,"end":121},"obj":"Antecedent"}],"relations":[{"id":"R768","pred":"boundBy","subj":"T426","obj":"T427"},{"id":"R777","pred":"boundBy","subj":"T426","obj":"T428"},{"id":"R778","pred":"boundBy","subj":"T426","obj":"T429"}],"namespaces":[{"prefix":"_base","uri":"https://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Background\nGATA-3 plays a critical role in regulating the expression of the cytokines interleukin (IL)-4, IL-5, and IL-13 from T helper-2 (Th2) cells and therefore is a key mediator of allergic diseases. Corticosteroids are highly effective in suppressing allergic inflammation, but their effects on GATA-3 are unknown. We investigated the effect of the corticosteroid fluticasone propionate on GATA-3 regulation in human T-lymphocytes in vitro and in vivo.\n\nMethods and Findings\nIn a T lymphocyte cell line (HuT-78) and peripheral blood mononuclear cells stimulated by anti-CD3 and anti-CD28 in vitro we demonstrated that fluticasone inhibits nuclear translocation of GATA-3 and expression of Th2 cytokines via a mechanism independent of nuclear factor-κB and is due, in part, to competition between GATA-3 and the ligand-activated glucocorticoid receptor for nuclear transport through the nuclear importer importin-α. In addition, fluticasone induces the expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1), the endogenous inhibitor of p38 MAPK, which is necessary for GATA-3 nuclear translocation. These inhibitory effects of fluticasone are rapid, potent, and prolonged. We also demonstrated that inhaled fluticasone inhibits GATA-3 nuclear translocation in peripheral blood lymphocytes of patients with asthma in vivo.\n\nConclusions\nCorticosteroids have a potent inhibitory effect on GATA-3 via two interacting mechanisms that potently suppress Th2 cytokine expression. This novel mechanism of action of corticosteroids may account for the striking clinical efficacy of corticosteroids in the treatment of allergic diseases.\n\nPlease see later in the article for Editors' Summary\n\n"}

bionlp-st-ge-2016-spacy-parsed

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T87","span":{"begin":532,"end":537},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"},{"id":"T88","span":{"begin":1297,"end":1302},"obj":"http://purl.obolibrary.org/obo/UBERON_0000178"}],"text":"Background\nGATA-3 plays a critical role in regulating the expression of the cytokines interleukin (IL)-4, IL-5, and IL-13 from T helper-2 (Th2) cells and therefore is a key mediator of allergic diseases. Corticosteroids are highly effective in suppressing allergic inflammation, but their effects on GATA-3 are unknown. We investigated the effect of the corticosteroid fluticasone propionate on GATA-3 regulation in human T-lymphocytes in vitro and in vivo.\n\nMethods and Findings\nIn a T lymphocyte cell line (HuT-78) and peripheral blood mononuclear cells stimulated by anti-CD3 and anti-CD28 in vitro we demonstrated that fluticasone inhibits nuclear translocation of GATA-3 and expression of Th2 cytokines via a mechanism independent of nuclear factor-κB and is due, in part, to competition between GATA-3 and the ligand-activated glucocorticoid receptor for nuclear transport through the nuclear importer importin-α. In addition, fluticasone induces the expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1), the endogenous inhibitor of p38 MAPK, which is necessary for GATA-3 nuclear translocation. These inhibitory effects of fluticasone are rapid, potent, and prolonged. We also demonstrated that inhaled fluticasone inhibits GATA-3 nuclear translocation in peripheral blood lymphocytes of patients with asthma in vivo.\n\nConclusions\nCorticosteroids have a potent inhibitory effect on GATA-3 via two interacting mechanisms that potently suppress Th2 cytokine expression. This novel mechanism of action of corticosteroids may account for the striking clinical efficacy of corticosteroids in the treatment of allergic diseases.\n\nPlease see later in the article for Editors' Summary\n\n"}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T757","span":{"begin":265,"end":277},"obj":"http://purl.obolibrary.org/obo/GO_0006954"},{"id":"T758","span":{"begin":402,"end":412},"obj":"http://purl.obolibrary.org/obo/GO_0065007"},{"id":"T759","span":{"begin":833,"end":868},"obj":"http://purl.obolibrary.org/obo/GO_0002147"},{"id":"T760","span":{"begin":861,"end":878},"obj":"http://purl.obolibrary.org/obo/GO_0051169"},{"id":"T761","span":{"begin":869,"end":878},"obj":"http://purl.obolibrary.org/obo/GO_0006810"},{"id":"T762","span":{"begin":979,"end":1003},"obj":"http://purl.obolibrary.org/obo/GO_0034199"},{"id":"T763","span":{"begin":979,"end":1003},"obj":"http://purl.obolibrary.org/obo/GO_0032147"},{"id":"T764","span":{"begin":979,"end":1003},"obj":"http://purl.obolibrary.org/obo/GO_0030295"},{"id":"T765","span":{"begin":979,"end":1003},"obj":"http://purl.obolibrary.org/obo/GO_0032111"},{"id":"T766","span":{"begin":1005,"end":1009},"obj":"http://purl.obolibrary.org/obo/GO_0004707"},{"id":"T767","span":{"begin":1062,"end":1065},"obj":"http://purl.obolibrary.org/obo/GO_0004707"},{"id":"T768","span":{"begin":1011,"end":1022},"obj":"http://purl.obolibrary.org/obo/GO_0016791"},{"id":"T755","span":{"begin":891,"end":907},"obj":"http://purl.obolibrary.org/obo/GO_0051170"}],"text":"Background\nGATA-3 plays a critical role in regulating the expression of the cytokines interleukin (IL)-4, IL-5, and IL-13 from T helper-2 (Th2) cells and therefore is a key mediator of allergic diseases. Corticosteroids are highly effective in suppressing allergic inflammation, but their effects on GATA-3 are unknown. We investigated the effect of the corticosteroid fluticasone propionate on GATA-3 regulation in human T-lymphocytes in vitro and in vivo.\n\nMethods and Findings\nIn a T lymphocyte cell line (HuT-78) and peripheral blood mononuclear cells stimulated by anti-CD3 and anti-CD28 in vitro we demonstrated that fluticasone inhibits nuclear translocation of GATA-3 and expression of Th2 cytokines via a mechanism independent of nuclear factor-κB and is due, in part, to competition between GATA-3 and the ligand-activated glucocorticoid receptor for nuclear transport through the nuclear importer importin-α. In addition, fluticasone induces the expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1), the endogenous inhibitor of p38 MAPK, which is necessary for GATA-3 nuclear translocation. These inhibitory effects of fluticasone are rapid, potent, and prolonged. We also demonstrated that inhaled fluticasone inhibits GATA-3 nuclear translocation in peripheral blood lymphocytes of patients with asthma in vivo.\n\nConclusions\nCorticosteroids have a potent inhibitory effect on GATA-3 via two interacting mechanisms that potently suppress Th2 cytokine expression. This novel mechanism of action of corticosteroids may account for the striking clinical efficacy of corticosteroids in the treatment of allergic diseases.\n\nPlease see later in the article for Editors' Summary\n\n"}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T770","span":{"begin":99,"end":104},"obj":"http://purl.obolibrary.org/obo/GO_0005136"},{"id":"T771","span":{"begin":106,"end":110},"obj":"http://purl.obolibrary.org/obo/GO_0005137"},{"id":"T772","span":{"begin":116,"end":121},"obj":"http://purl.obolibrary.org/obo/GO_0005144"},{"id":"T773","span":{"begin":816,"end":822},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T774","span":{"begin":979,"end":1003},"obj":"http://purl.obolibrary.org/obo/GO_0030295"},{"id":"T775","span":{"begin":1005,"end":1009},"obj":"http://purl.obolibrary.org/obo/GO_0004707"},{"id":"T776","span":{"begin":1062,"end":1065},"obj":"http://purl.obolibrary.org/obo/GO_0004707"},{"id":"T777","span":{"begin":1011,"end":1022},"obj":"http://purl.obolibrary.org/obo/GO_0016791"}],"text":"Background\nGATA-3 plays a critical role in regulating the expression of the cytokines interleukin (IL)-4, IL-5, and IL-13 from T helper-2 (Th2) cells and therefore is a key mediator of allergic diseases. Corticosteroids are highly effective in suppressing allergic inflammation, but their effects on GATA-3 are unknown. We investigated the effect of the corticosteroid fluticasone propionate on GATA-3 regulation in human T-lymphocytes in vitro and in vivo.\n\nMethods and Findings\nIn a T lymphocyte cell line (HuT-78) and peripheral blood mononuclear cells stimulated by anti-CD3 and anti-CD28 in vitro we demonstrated that fluticasone inhibits nuclear translocation of GATA-3 and expression of Th2 cytokines via a mechanism independent of nuclear factor-κB and is due, in part, to competition between GATA-3 and the ligand-activated glucocorticoid receptor for nuclear transport through the nuclear importer importin-α. In addition, fluticasone induces the expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1), the endogenous inhibitor of p38 MAPK, which is necessary for GATA-3 nuclear translocation. These inhibitory effects of fluticasone are rapid, potent, and prolonged. We also demonstrated that inhaled fluticasone inhibits GATA-3 nuclear translocation in peripheral blood lymphocytes of patients with asthma in vivo.\n\nConclusions\nCorticosteroids have a potent inhibitory effect on GATA-3 via two interacting mechanisms that potently suppress Th2 cytokine expression. This novel mechanism of action of corticosteroids may account for the striking clinical efficacy of corticosteroids in the treatment of allergic diseases.\n\nPlease see later in the article for Editors' Summary\n\n"}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T778","span":{"begin":144,"end":149},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T779","span":{"begin":550,"end":555},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Background\nGATA-3 plays a critical role in regulating the expression of the cytokines interleukin (IL)-4, IL-5, and IL-13 from T helper-2 (Th2) cells and therefore is a key mediator of allergic diseases. Corticosteroids are highly effective in suppressing allergic inflammation, but their effects on GATA-3 are unknown. We investigated the effect of the corticosteroid fluticasone propionate on GATA-3 regulation in human T-lymphocytes in vitro and in vivo.\n\nMethods and Findings\nIn a T lymphocyte cell line (HuT-78) and peripheral blood mononuclear cells stimulated by anti-CD3 and anti-CD28 in vitro we demonstrated that fluticasone inhibits nuclear translocation of GATA-3 and expression of Th2 cytokines via a mechanism independent of nuclear factor-κB and is due, in part, to competition between GATA-3 and the ligand-activated glucocorticoid receptor for nuclear transport through the nuclear importer importin-α. In addition, fluticasone induces the expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1), the endogenous inhibitor of p38 MAPK, which is necessary for GATA-3 nuclear translocation. These inhibitory effects of fluticasone are rapid, potent, and prolonged. We also demonstrated that inhaled fluticasone inhibits GATA-3 nuclear translocation in peripheral blood lymphocytes of patients with asthma in vivo.\n\nConclusions\nCorticosteroids have a potent inhibitory effect on GATA-3 via two interacting mechanisms that potently suppress Th2 cytokine expression. This novel mechanism of action of corticosteroids may account for the striking clinical efficacy of corticosteroids in the treatment of allergic diseases.\n\nPlease see later in the article for Editors' Summary\n\n"}

sentences

{"project":"sentences","denotations":[{"id":"T136","span":{"begin":0,"end":10},"obj":"Sentence"},{"id":"T137","span":{"begin":11,"end":203},"obj":"Sentence"},{"id":"T138","span":{"begin":204,"end":319},"obj":"Sentence"},{"id":"T139","span":{"begin":320,"end":457},"obj":"Sentence"},{"id":"T140","span":{"begin":459,"end":479},"obj":"Sentence"},{"id":"T141","span":{"begin":480,"end":919},"obj":"Sentence"},{"id":"T142","span":{"begin":920,"end":1124},"obj":"Sentence"},{"id":"T143","span":{"begin":1125,"end":1198},"obj":"Sentence"},{"id":"T144","span":{"begin":1199,"end":1347},"obj":"Sentence"},{"id":"T145","span":{"begin":1349,"end":1360},"obj":"Sentence"},{"id":"T146","span":{"begin":1361,"end":1497},"obj":"Sentence"},{"id":"T147","span":{"begin":1498,"end":1652},"obj":"Sentence"},{"id":"T148","span":{"begin":1654,"end":1706},"obj":"Sentence"},{"id":"T6","span":{"begin":0,"end":10},"obj":"Sentence"},{"id":"T7","span":{"begin":11,"end":203},"obj":"Sentence"},{"id":"T8","span":{"begin":204,"end":319},"obj":"Sentence"},{"id":"T9","span":{"begin":320,"end":457},"obj":"Sentence"},{"id":"T10","span":{"begin":459,"end":479},"obj":"Sentence"},{"id":"T11","span":{"begin":480,"end":919},"obj":"Sentence"},{"id":"T12","span":{"begin":920,"end":1124},"obj":"Sentence"},{"id":"T13","span":{"begin":1125,"end":1198},"obj":"Sentence"},{"id":"T14","span":{"begin":1199,"end":1347},"obj":"Sentence"},{"id":"T15","span":{"begin":1349,"end":1360},"obj":"Sentence"},{"id":"T16","span":{"begin":1361,"end":1497},"obj":"Sentence"},{"id":"T17","span":{"begin":1498,"end":1652},"obj":"Sentence"},{"id":"T18","span":{"begin":1654,"end":1706},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Background\nGATA-3 plays a critical role in regulating the expression of the cytokines interleukin (IL)-4, IL-5, and IL-13 from T helper-2 (Th2) cells and therefore is a key mediator of allergic diseases. Corticosteroids are highly effective in suppressing allergic inflammation, but their effects on GATA-3 are unknown. We investigated the effect of the corticosteroid fluticasone propionate on GATA-3 regulation in human T-lymphocytes in vitro and in vivo.\n\nMethods and Findings\nIn a T lymphocyte cell line (HuT-78) and peripheral blood mononuclear cells stimulated by anti-CD3 and anti-CD28 in vitro we demonstrated that fluticasone inhibits nuclear translocation of GATA-3 and expression of Th2 cytokines via a mechanism independent of nuclear factor-κB and is due, in part, to competition between GATA-3 and the ligand-activated glucocorticoid receptor for nuclear transport through the nuclear importer importin-α. In addition, fluticasone induces the expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1), the endogenous inhibitor of p38 MAPK, which is necessary for GATA-3 nuclear translocation. These inhibitory effects of fluticasone are rapid, potent, and prolonged. We also demonstrated that inhaled fluticasone inhibits GATA-3 nuclear translocation in peripheral blood lymphocytes of patients with asthma in vivo.\n\nConclusions\nCorticosteroids have a potent inhibitory effect on GATA-3 via two interacting mechanisms that potently suppress Th2 cytokine expression. This novel mechanism of action of corticosteroids may account for the striking clinical efficacy of corticosteroids in the treatment of allergic diseases.\n\nPlease see later in the article for Editors' Summary\n\n"}

ICD10

{"project":"ICD10","denotations":[{"id":"T780","span":{"begin":1332,"end":1338},"obj":"http://purl.bioontology.org/ontology/ICD10/J45"},{"id":"T769","span":{"begin":1332,"end":1338},"obj":"http://purl.bioontology.org/ontology/ICD10/J45.9"}],"text":"Background\nGATA-3 plays a critical role in regulating the expression of the cytokines interleukin (IL)-4, IL-5, and IL-13 from T helper-2 (Th2) cells and therefore is a key mediator of allergic diseases. Corticosteroids are highly effective in suppressing allergic inflammation, but their effects on GATA-3 are unknown. We investigated the effect of the corticosteroid fluticasone propionate on GATA-3 regulation in human T-lymphocytes in vitro and in vivo.\n\nMethods and Findings\nIn a T lymphocyte cell line (HuT-78) and peripheral blood mononuclear cells stimulated by anti-CD3 and anti-CD28 in vitro we demonstrated that fluticasone inhibits nuclear translocation of GATA-3 and expression of Th2 cytokines via a mechanism independent of nuclear factor-κB and is due, in part, to competition between GATA-3 and the ligand-activated glucocorticoid receptor for nuclear transport through the nuclear importer importin-α. In addition, fluticasone induces the expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1), the endogenous inhibitor of p38 MAPK, which is necessary for GATA-3 nuclear translocation. These inhibitory effects of fluticasone are rapid, potent, and prolonged. We also demonstrated that inhaled fluticasone inhibits GATA-3 nuclear translocation in peripheral blood lymphocytes of patients with asthma in vivo.\n\nConclusions\nCorticosteroids have a potent inhibitory effect on GATA-3 via two interacting mechanisms that potently suppress Th2 cytokine expression. This novel mechanism of action of corticosteroids may account for the striking clinical efficacy of corticosteroids in the treatment of allergic diseases.\n\nPlease see later in the article for Editors' Summary\n\n"}

events-check-again

bionlp-st-ge-2016-reference-tees

bionlp-st-ge-2016-reference

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T431","span":{"begin":11,"end":17},"obj":"P23771"},{"id":"T432","span":{"begin":106,"end":110},"obj":"P05113"},{"id":"T433","span":{"begin":116,"end":121},"obj":"P35225"},{"id":"T434","span":{"begin":300,"end":306},"obj":"P23771"},{"id":"T435","span":{"begin":395,"end":401},"obj":"P23771"},{"id":"T436","span":{"begin":575,"end":578},"obj":"P04234"},{"id":"T437","span":{"begin":575,"end":578},"obj":"P20963"},{"id":"T438","span":{"begin":575,"end":578},"obj":"P09693"},{"id":"T439","span":{"begin":575,"end":578},"obj":"P07766"},{"id":"T440","span":{"begin":588,"end":592},"obj":"P10747"},{"id":"T441","span":{"begin":669,"end":675},"obj":"P23771"},{"id":"T442","span":{"begin":801,"end":807},"obj":"P23771"},{"id":"T443","span":{"begin":833,"end":856},"obj":"P04150"},{"id":"T444","span":{"begin":971,"end":1003},"obj":"P27361"},{"id":"T445","span":{"begin":971,"end":1003},"obj":"P28482"},{"id":"T446","span":{"begin":1026,"end":1031},"obj":"P28562"},{"id":"T447","span":{"begin":1062,"end":1065},"obj":"P53778"},{"id":"T448","span":{"begin":1062,"end":1065},"obj":"Q16539"},{"id":"T449","span":{"begin":1062,"end":1065},"obj":"Q15759"},{"id":"T450","span":{"begin":1062,"end":1065},"obj":"O15264"},{"id":"T451","span":{"begin":1095,"end":1101},"obj":"P23771"},{"id":"T452","span":{"begin":1254,"end":1260},"obj":"P23771"},{"id":"T453","span":{"begin":1412,"end":1418},"obj":"P23771"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Background\nGATA-3 plays a critical role in regulating the expression of the cytokines interleukin (IL)-4, IL-5, and IL-13 from T helper-2 (Th2) cells and therefore is a key mediator of allergic diseases. Corticosteroids are highly effective in suppressing allergic inflammation, but their effects on GATA-3 are unknown. We investigated the effect of the corticosteroid fluticasone propionate on GATA-3 regulation in human T-lymphocytes in vitro and in vivo.\n\nMethods and Findings\nIn a T lymphocyte cell line (HuT-78) and peripheral blood mononuclear cells stimulated by anti-CD3 and anti-CD28 in vitro we demonstrated that fluticasone inhibits nuclear translocation of GATA-3 and expression of Th2 cytokines via a mechanism independent of nuclear factor-κB and is due, in part, to competition between GATA-3 and the ligand-activated glucocorticoid receptor for nuclear transport through the nuclear importer importin-α. In addition, fluticasone induces the expression of mitogen-activated protein kinase (MAPK) phosphatase-1 (MKP-1), the endogenous inhibitor of p38 MAPK, which is necessary for GATA-3 nuclear translocation. These inhibitory effects of fluticasone are rapid, potent, and prolonged. We also demonstrated that inhaled fluticasone inhibits GATA-3 nuclear translocation in peripheral blood lymphocytes of patients with asthma in vivo.\n\nConclusions\nCorticosteroids have a potent inhibitory effect on GATA-3 via two interacting mechanisms that potently suppress Th2 cytokine expression. This novel mechanism of action of corticosteroids may account for the striking clinical efficacy of corticosteroids in the treatment of allergic diseases.\n\nPlease see later in the article for Editors' Summary\n\n"}

test2

PMC:2674207 / 455-2163 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:2674207 / 455-2163 JSON TXT