| Id |
Subject |
Object |
Predicate |
Lexical cue |
| T35 |
0-37 |
Sentence |
denotes |
What Did the Researchers Do and Find? |
| T36 |
38-277 |
Sentence |
denotes |
Transcription factors have to move into the nucleus of cells (so-called nuclear translocation) to control the expression of their target genes, so the researchers first asked whether fluticasone affects the cellular localization of GATA-3. |
| T37 |
278-441 |
Sentence |
denotes |
Fluticasone treatment of activated T lymphocytes growing in dishes, they report, inhibited the nuclear translocation of GATA-3 and reduced Th2 cytokine expression. |
| T38 |
442-693 |
Sentence |
denotes |
Other experiments showed that the inhibition of GATA-3 nuclear translocation was partly caused by competition between the glucocorticoid receptor bound to fluticasone and GATA-3 for binding to importin-α, a protein that is required for nuclear import. |
| T39 |
694-782 |
Sentence |
denotes |
However, fluticasone also prevented the nuclear translocation of GATA-3 in a second way. |
| T40 |
783-883 |
Sentence |
denotes |
Before GATA-3 can bind to importin-α, phosphate groups have to be added to specific sites in GATA-3. |
| T41 |
884-1107 |
Sentence |
denotes |
This “phosphorylation” requires an enzyme called p38 MAP kinase, and the researchers found that fluticasone treatment of activated T lymphocytes induced the expression of MAP kinase phophatase-1, a p38 MAP kinase inhibitor. |
| T42 |
1108-1330 |
Sentence |
denotes |
Finally, when the researchers treated seven patients with mild asthma with inhaled fluticasone, they found that fluticasone also inhibited GATA-3 nuclear translocation in the lymphocytes circulating in the patients' blood. |
