PMC:2674207 / 20723-21171
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pmc-enju-pas
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bionlp-st-ge-2016-spacy-parsed
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UBERON-AE
{"project":"UBERON-AE","denotations":[{"id":"T7168","span":{"begin":192,"end":197},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"Transfection\nHuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection. The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed."}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T7341","span":{"begin":20,"end":25},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7342","span":{"begin":136,"end":141},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7343","span":{"begin":360,"end":365},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T7344","span":{"begin":417,"end":421},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"Transfection\nHuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection. The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed."}
sentences
{"project":"sentences","denotations":[{"id":"T7169","span":{"begin":0,"end":12},"obj":"Sentence"},{"id":"T7170","span":{"begin":13,"end":284},"obj":"Sentence"},{"id":"T7171","span":{"begin":285,"end":448},"obj":"Sentence"},{"id":"T147","span":{"begin":0,"end":12},"obj":"Sentence"},{"id":"T148","span":{"begin":13,"end":284},"obj":"Sentence"},{"id":"T149","span":{"begin":285,"end":448},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Transfection\nHuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection. The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed."}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T7345","span":{"begin":380,"end":388},"obj":"Protein"},{"id":"T7346","span":{"begin":389,"end":393},"obj":"Protein"}],"text":"Transfection\nHuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection. The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T7248","span":{"begin":62,"end":65},"obj":"Q9U6Y4"},{"id":"T7249","span":{"begin":385,"end":388},"obj":"P20963"},{"id":"T7250","span":{"begin":385,"end":388},"obj":"P09693"},{"id":"T7251","span":{"begin":385,"end":388},"obj":"P07766"},{"id":"T7252","span":{"begin":385,"end":388},"obj":"P04234"},{"id":"T7253","span":{"begin":389,"end":393},"obj":"P10747"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Transfection\nHuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection. The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed."}