PMC:2674207 / 20723-21171
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7172 | 0-12 | NN | denotes | Transfection |
T7173 | 13-19 | NN | denotes | HuT-78 |
T7174 | 20-25 | NN | denotes | cells |
T7175 | 26-30 | VB | denotes | were |
T7176 | 31-42 | VB | denotes | transfected |
T7177 | 43-47 | IN | denotes | with |
T7178 | 48-54 | CC | denotes | either |
T7179 | 55-58 | NN | denotes | EP8 |
T7180 | 59-61 | CC | denotes | or |
T7181 | 62-65 | NN | denotes | GFP |
T7182 | 66-72 | NN | denotes | vector |
T7183 | 73-77 | RB | denotes | only |
T7184 | 78-81 | NN | denotes | DNA |
T7185 | 82-87 | VB | denotes | using |
T7186 | 88-96 | NN | denotes | solution |
T7187 | 97-98 | NNP | denotes | R |
T7188 | 98-99 | -COMMA- | denotes | , |
T7189 | 100-109 | NN | denotes | programme |
T7190 | 110-115 | NN | denotes | V-001 |
T7191 | 116-118 | IN | denotes | at |
T7192 | 119-120 | DT | denotes | a |
T7193 | 121-126 | NN | denotes | ratio |
T7194 | 127-129 | IN | denotes | of |
T7195 | 130-135 | CD | denotes | 3×106 |
T7196 | 136-143 | NN | denotes | cells/4 |
T7197 | 144-146 | VB | denotes | µg |
T7198 | 147-150 | NN | denotes | DNA |
T7199 | 151-154 | IN | denotes | for |
T7200 | 155-158 | CD | denotes | 7–8 |
T7201 | 159-160 | NN | denotes | h |
T7202 | 161-163 | IN | denotes | in |
T7203 | 164-172 | JJ | denotes | complete |
T7204 | 173-179 | NN | denotes | medium |
T7205 | 180-181 | -LRB- | denotes | ( |
T7206 | 181-183 | CD | denotes | 10 |
T7207 | 183-184 | NN | denotes | % |
T7208 | 185-191 | JJ | denotes | bovine |
T7209 | 192-197 | NN | denotes | serum |
T7210 | 198-200 | IN | denotes | in |
T7211 | 201-212 | NN | denotes | RPMI1640+15 |
T7212 | 213-224 | NN | denotes | L-glutamine |
T7213 | 224-225 | -RRB- | denotes | ) |
T7214 | 226-235 | VB | denotes | according |
T7215 | 236-238 | TO | denotes | to |
T7216 | 239-242 | DT | denotes | the |
T7217 | 243-250 | JJ | denotes | general |
T7218 | 251-256 | NNP | denotes | Amaxa |
T7219 | 257-265 | NN | denotes | protocol |
T7220 | 266-269 | IN | denotes | for |
T7221 | 270-283 | NN | denotes | nucleofection |
T7222 | 285-288 | DT | denotes | The |
T7223 | 289-295 | NN | denotes | medium |
T7224 | 296-299 | VB | denotes | was |
T7225 | 300-312 | RB | denotes | subsequently |
T7226 | 313-320 | VB | denotes | changed |
T7227 | 321-323 | TO | denotes | to |
T7228 | 324-325 | CD | denotes | 1 |
T7229 | 325-326 | NN | denotes | % |
T7230 | 327-331 | NN | denotes | RPMI |
T7231 | 332-335 | IN | denotes | for |
T7232 | 336-338 | CD | denotes | 24 |
T7233 | 339-340 | NN | denotes | h |
T7234 | 341-347 | IN | denotes | before |
T7235 | 348-359 | VB | denotes | transfected |
T7236 | 360-365 | NN | denotes | cells |
T7237 | 366-370 | VB | denotes | were |
T7238 | 371-376 | VB | denotes | added |
T7239 | 377-379 | TO | denotes | to |
T7240 | 380-393 | JJ | denotes | anti-CD3/CD28 |
T7241 | 394-401 | VB | denotes | treated |
T7242 | 402-407 | NN | denotes | wells |
T7243 | 408-411 | CC | denotes | and |
T7244 | 412-416 | JJ | denotes | live |
T7245 | 417-421 | NN | denotes | cell |
T7246 | 422-437 | NN | denotes | videomicroscopy |
T7247 | 438-447 | VB | denotes | performed |
R6131 | T7174 | T7173 | arg1Of | cells,HuT-78 |
R6132 | T7174 | T7175 | arg1Of | cells,were |
R6133 | T7174 | T7176 | arg2Of | cells,transfected |
R6134 | T7176 | T7175 | arg2Of | transfected,were |
R6135 | T7176 | T7177 | arg1Of | transfected,with |
R6136 | T7179 | T7180 | arg1Of | EP8,or |
R6137 | T7180 | T7177 | arg2Of | or,with |
R6138 | T7180 | T7178 | arg1Of | or,either |
R6139 | T7182 | T7180 | arg2Of | vector,or |
R6140 | T7182 | T7181 | arg1Of | vector,GFP |
R6141 | T7184 | T7183 | arg1Of | DNA,only |
R6142 | T7184 | T7185 | arg1Of | DNA,using |
R6143 | T7184 | T7197 | arg1Of | DNA,µg |
R6144 | T7187 | T7185 | arg2Of | R,using |
R6145 | T7187 | T7186 | arg1Of | R,solution |
R6146 | T7187 | T7188 | arg1Of | R,"," |
R6147 | T7190 | T7188 | arg2Of | V-001,"," |
R6148 | T7190 | T7189 | arg1Of | V-001,programme |
R6149 | T7190 | T7191 | arg1Of | V-001,at |
R6150 | T7193 | T7191 | arg2Of | ratio,at |
R6151 | T7193 | T7192 | arg1Of | ratio,a |
R6152 | T7193 | T7194 | arg1Of | ratio,of |
R6153 | T7196 | T7194 | arg2Of | cells/4,of |
R6154 | T7196 | T7195 | arg1Of | cells/4,3×106 |
R6155 | T7197 | T7175 | modOf | µg,were |
R6156 | T7197 | T7199 | arg1Of | µg,for |
R6157 | T7197 | T7202 | arg1Of | µg,in |
R6158 | T7197 | T7214 | arg1Of | µg,according |
R6159 | T7198 | T7197 | arg2Of | DNA,µg |
R6160 | T7201 | T7199 | arg2Of | h,for |
R6161 | T7201 | T7200 | arg1Of | h,7–8 |
R6162 | T7204 | T7202 | arg2Of | medium,in |
R6163 | T7204 | T7203 | arg1Of | medium,complete |
R6164 | T7204 | T7205 | arg1Of | medium,( |
R6165 | T7206 | T7207 | arg1Of | 10,% |
R6166 | T7209 | T7205 | arg2Of | serum,( |
R6167 | T7209 | T7206 | arg1Of | serum,10 |
R6168 | T7209 | T7208 | arg1Of | serum,bovine |
R6169 | T7209 | T7210 | arg1Of | serum,in |
R6170 | T7212 | T7210 | arg2Of | L-glutamine,in |
R6171 | T7212 | T7211 | arg1Of | L-glutamine,RPMI1640+15 |
R6172 | T7213 | T7205 | arg3Of | ),( |
R6173 | T7215 | T7214 | arg2Of | to,according |
R6174 | T7219 | T7215 | arg2Of | protocol,to |
R6175 | T7219 | T7216 | arg1Of | protocol,the |
R6176 | T7219 | T7217 | arg1Of | protocol,general |
R6177 | T7219 | T7218 | arg1Of | protocol,Amaxa |
R6178 | T7219 | T7220 | arg1Of | protocol,for |
R6179 | T7221 | T7220 | arg2Of | nucleofection,for |
R6180 | T7223 | T7222 | arg1Of | medium,The |
R6181 | T7223 | T7224 | arg1Of | medium,was |
R6182 | T7223 | T7226 | arg2Of | medium,changed |
R6183 | T7226 | T7224 | arg2Of | changed,was |
R6184 | T7226 | T7225 | arg1Of | changed,subsequently |
R6185 | T7226 | T7227 | arg1Of | changed,to |
R6186 | T7226 | T7231 | arg1Of | changed,for |
R6187 | T7228 | T7229 | arg1Of | 1,% |
R6188 | T7230 | T7227 | arg2Of | RPMI,to |
R6189 | T7230 | T7228 | arg1Of | RPMI,1 |
R6190 | T7233 | T7232 | arg1Of | h,24 |
R6191 | T7233 | T7234 | arg1Of | h,before |
R6192 | T7233 | T7243 | arg1Of | h,and |
R6193 | T7236 | T7235 | arg2Of | cells,transfected |
R6194 | T7236 | T7237 | arg1Of | cells,were |
R6195 | T7236 | T7238 | arg2Of | cells,added |
R6196 | T7238 | T7234 | arg2Of | added,before |
R6197 | T7238 | T7237 | arg2Of | added,were |
R6198 | T7238 | T7239 | arg1Of | added,to |
R6199 | T7242 | T7239 | arg2Of | wells,to |
R6200 | T7242 | T7240 | arg1Of | wells,anti-CD3/CD28 |
R6201 | T7242 | T7241 | arg2Of | wells,treated |
R6202 | T7243 | T7231 | arg2Of | and,for |
R6203 | T7246 | T7243 | arg2Of | videomicroscopy,and |
R6204 | T7246 | T7244 | arg1Of | videomicroscopy,live |
R6205 | T7246 | T7245 | arg1Of | videomicroscopy,cell |
R6206 | T7246 | T7247 | arg2Of | videomicroscopy,performed |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7254 | 0-12 | NNP | denotes | Transfection |
T7255 | 13-19 | NNP | denotes | HuT-78 |
T7256 | 20-25 | NNS | denotes | cells |
T7257 | 26-30 | VBD | denotes | were |
T7258 | 31-42 | VBN | denotes | transfected |
T7259 | 43-47 | IN | denotes | with |
T7260 | 48-54 | DT | denotes | either |
T7261 | 55-58 | CD | denotes | EP8 |
T7262 | 59-61 | CC | denotes | or |
T7263 | 62-65 | NNP | denotes | GFP |
T7264 | 66-72 | NN | denotes | vector |
T7265 | 73-77 | RB | denotes | only |
T7266 | 78-81 | NNP | denotes | DNA |
T7267 | 82-87 | VBG | denotes | using |
T7268 | 88-96 | NN | denotes | solution |
T7269 | 97-98 | NN | denotes | R |
T7270 | 98-99 | , | denotes | , |
T7271 | 100-109 | NN | denotes | programme |
T7272 | 110-115 | NN | denotes | V-001 |
T7273 | 116-118 | IN | denotes | at |
T7274 | 119-120 | DT | denotes | a |
T7275 | 121-126 | NN | denotes | ratio |
T7276 | 127-129 | IN | denotes | of |
T7277 | 130-131 | CD | denotes | 3 |
T7278 | 131-132 | CD | denotes | × |
T7279 | 132-135 | CD | denotes | 106 |
T7280 | 136-143 | CD | denotes | cells/4 |
T7281 | 144-146 | NN | denotes | µg |
T7282 | 147-150 | NN | denotes | DNA |
T7283 | 151-154 | IN | denotes | for |
T7284 | 155-156 | CD | denotes | 7 |
T7285 | 157-158 | CD | denotes | 8 |
T7286 | 159-160 | NN | denotes | h |
T7287 | 161-163 | IN | denotes | in |
T7288 | 164-172 | JJ | denotes | complete |
T7289 | 173-179 | NN | denotes | medium |
T7290 | 180-181 | -LRB- | denotes | ( |
T7291 | 181-183 | CD | denotes | 10 |
T7292 | 183-184 | NN | denotes | % |
T7293 | 185-191 | JJ | denotes | bovine |
T7294 | 192-197 | NN | denotes | serum |
T7295 | 198-200 | IN | denotes | in |
T7296 | 201-209 | NNP | denotes | RPMI1640 |
T7297 | 209-212 | CD | denotes | +15 |
T7298 | 213-224 | NN | denotes | L-glutamine |
T7299 | 224-225 | -RRB- | denotes | ) |
T7300 | 226-235 | VBG | denotes | according |
T7301 | 236-238 | TO | denotes | to |
T7302 | 239-242 | DT | denotes | the |
T7303 | 243-250 | JJ | denotes | general |
T7304 | 251-256 | NNP | denotes | Amaxa |
T7305 | 257-265 | NN | denotes | protocol |
T7306 | 266-269 | IN | denotes | for |
T7307 | 270-283 | NN | denotes | nucleofection |
T7308 | 283-284 | . | denotes | . |
T7309 | 285-288 | DT | denotes | The |
T7310 | 289-295 | NN | denotes | medium |
T7311 | 296-299 | VBD | denotes | was |
T7312 | 300-312 | RB | denotes | subsequently |
T7313 | 313-320 | VBN | denotes | changed |
T7314 | 321-323 | TO | denotes | to |
T7315 | 324-325 | CD | denotes | 1 |
T7316 | 325-326 | NN | denotes | % |
T7317 | 327-331 | NNP | denotes | RPMI |
T7318 | 332-335 | IN | denotes | for |
T7319 | 336-338 | CD | denotes | 24 |
T7320 | 339-340 | NN | denotes | h |
T7321 | 341-347 | IN | denotes | before |
T7322 | 348-359 | VBN | denotes | transfected |
T7323 | 360-365 | NNS | denotes | cells |
T7324 | 366-370 | VBD | denotes | were |
T7325 | 371-376 | VBN | denotes | added |
T7326 | 377-379 | TO | denotes | to |
T7327 | 380-388 | JJ | denotes | anti-CD3 |
T7328 | 388-389 | NN | denotes | / |
T7329 | 389-393 | CD | denotes | CD28 |
T7330 | 394-401 | VBN | denotes | treated |
T7331 | 402-407 | NNS | denotes | wells |
T7332 | 408-411 | CC | denotes | and |
T7333 | 412-416 | VBP | denotes | live |
T7334 | 417-421 | NN | denotes | cell |
T7335 | 422-437 | JJ | denotes | videomicroscopy |
T7336 | 438-447 | VBN | denotes | performed |
T7337 | 447-448 | . | denotes | . |
R6247 | T7294 | T7286 | appos | serum,h |
R6248 | T7295 | T7294 | prep | in,serum |
R6249 | T7296 | T7295 | pobj | RPMI1640,in |
R6250 | T7297 | T7298 | nummod | +15,L-glutamine |
R6251 | T7298 | T7294 | appos | L-glutamine,serum |
R6252 | T7299 | T7286 | punct | ),h |
R6253 | T7300 | T7258 | prep | according,transfected |
R6254 | T7301 | T7300 | prep | to,according |
R6255 | T7302 | T7305 | det | the,protocol |
R6256 | T7303 | T7305 | amod | general,protocol |
R6257 | T7304 | T7305 | compound | Amaxa,protocol |
R6258 | T7305 | T7301 | pobj | protocol,to |
R6207 | T7254 | T7255 | compound | Transfection,HuT-78 |
R6208 | T7255 | T7256 | compound | HuT-78,cells |
R6209 | T7256 | T7258 | nsubjpass | cells,transfected |
R6210 | T7257 | T7258 | auxpass | were,transfected |
R6211 | T7258 | T7258 | ROOT | transfected,transfected |
R6212 | T7259 | T7258 | prep | with,transfected |
R6213 | T7260 | T7261 | det | either,EP8 |
R6214 | T7261 | T7259 | pobj | EP8,with |
R6215 | T7262 | T7261 | cc | or,EP8 |
R6216 | T7263 | T7264 | compound | GFP,vector |
R6217 | T7264 | T7261 | conj | vector,EP8 |
R6218 | T7265 | T7266 | advmod | only,DNA |
R6219 | T7266 | T7267 | nsubj | DNA,using |
R6220 | T7267 | T7258 | advcl | using,transfected |
R6221 | T7268 | T7269 | compound | solution,R |
R6222 | T7269 | T7267 | dobj | R,using |
R6223 | T7270 | T7269 | punct | ",",R |
R6224 | T7271 | T7272 | compound | programme,V-001 |
R6225 | T7272 | T7269 | appos | V-001,R |
R6226 | T7273 | T7267 | prep | at,using |
R6227 | T7274 | T7275 | det | a,ratio |
R6228 | T7275 | T7282 | nmod | ratio,DNA |
R6229 | T7276 | T7275 | prep | of,ratio |
R6230 | T7277 | T7280 | nummod | 3,cells/4 |
R6231 | T7278 | T7280 | nummod | ×,cells/4 |
R6232 | T7279 | T7280 | nummod | 106,cells/4 |
R6233 | T7280 | T7276 | pobj | cells/4,of |
R6234 | T7281 | T7282 | compound | µg,DNA |
R6235 | T7282 | T7273 | pobj | DNA,at |
R6236 | T7283 | T7267 | prep | for,using |
R6237 | T7284 | T7283 | pobj | 7,for |
R6238 | T7285 | T7286 | nummod | 8,h |
R6239 | T7286 | T7284 | appos | h,7 |
R6240 | T7287 | T7286 | prep | in,h |
R6241 | T7288 | T7289 | amod | complete,medium |
R6242 | T7289 | T7287 | pobj | medium,in |
R6243 | T7290 | T7286 | punct | (,h |
R6244 | T7291 | T7292 | nummod | 10,% |
R6245 | T7292 | T7294 | nmod | %,serum |
R6246 | T7293 | T7294 | amod | bovine,serum |
R6259 | T7306 | T7305 | prep | for,protocol |
R6260 | T7307 | T7306 | pobj | nucleofection,for |
R6261 | T7308 | T7258 | punct | .,transfected |
R6262 | T7309 | T7310 | det | The,medium |
R6263 | T7310 | T7313 | nsubjpass | medium,changed |
R6264 | T7311 | T7313 | auxpass | was,changed |
R6265 | T7312 | T7313 | advmod | subsequently,changed |
R6266 | T7313 | T7313 | ROOT | changed,changed |
R6267 | T7314 | T7313 | prep | to,changed |
R6268 | T7315 | T7317 | nummod | 1,RPMI |
R6269 | T7316 | T7315 | quantmod | %,1 |
R6270 | T7317 | T7314 | pobj | RPMI,to |
R6271 | T7318 | T7313 | prep | for,changed |
R6272 | T7319 | T7320 | nummod | 24,h |
R6273 | T7320 | T7318 | pobj | h,for |
R6274 | T7321 | T7325 | mark | before,added |
R6275 | T7322 | T7323 | amod | transfected,cells |
R6276 | T7323 | T7325 | nsubjpass | cells,added |
R6277 | T7324 | T7325 | auxpass | were,added |
R6278 | T7325 | T7313 | advcl | added,changed |
R6279 | T7326 | T7325 | prep | to,added |
R6280 | T7327 | T7336 | amod | anti-CD3,performed |
R6281 | T7328 | T7331 | nummod | /,wells |
R6282 | T7329 | T7331 | nummod | CD28,wells |
R6283 | T7330 | T7331 | amod | treated,wells |
R6284 | T7331 | T7327 | dobj | wells,anti-CD3 |
R6285 | T7332 | T7327 | cc | and,anti-CD3 |
R6286 | T7333 | T7335 | amod | live,videomicroscopy |
R6287 | T7334 | T7335 | compound | cell,videomicroscopy |
R6288 | T7335 | T7327 | conj | videomicroscopy,anti-CD3 |
R6289 | T7336 | T7326 | pobj | performed,to |
R6290 | T7337 | T7313 | punct | .,changed |
UBERON-AE
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7168 | 192-197 | http://purl.obolibrary.org/obo/UBERON_0001977 | denotes | serum |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7341 | 20-25 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T7342 | 136-141 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T7343 | 360-365 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T7344 | 417-421 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cell |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7169 | 0-12 | Sentence | denotes | Transfection |
T7170 | 13-284 | Sentence | denotes | HuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection. |
T7171 | 285-448 | Sentence | denotes | The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed. |
T147 | 0-12 | Sentence | denotes | Transfection |
T148 | 13-284 | Sentence | denotes | HuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection. |
T149 | 285-448 | Sentence | denotes | The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed. |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7345 | 380-388 | Protein | denotes | anti-CD3 |
T7346 | 389-393 | Protein | denotes | CD28 |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T7248 | 62-65 | Q9U6Y4 | denotes | GFP |
T7249 | 385-388 | P20963 | denotes | CD3 |
T7250 | 385-388 | P09693 | denotes | CD3 |
T7251 | 385-388 | P07766 | denotes | CD3 |
T7252 | 385-388 | P04234 | denotes | CD3 |
T7253 | 389-393 | P10747 | denotes | CD28 |