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PMC:2674207 / 20723-21171 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T7172 0-12 NN denotes Transfection
T7173 13-19 NN denotes HuT-78
T7174 20-25 NN denotes cells
T7175 26-30 VB denotes were
T7176 31-42 VB denotes transfected
T7177 43-47 IN denotes with
T7178 48-54 CC denotes either
T7179 55-58 NN denotes EP8
T7180 59-61 CC denotes or
T7181 62-65 NN denotes GFP
T7182 66-72 NN denotes vector
T7183 73-77 RB denotes only
T7184 78-81 NN denotes DNA
T7185 82-87 VB denotes using
T7186 88-96 NN denotes solution
T7187 97-98 NNP denotes R
T7188 98-99 -COMMA- denotes ,
T7189 100-109 NN denotes programme
T7190 110-115 NN denotes V-001
T7191 116-118 IN denotes at
T7192 119-120 DT denotes a
T7193 121-126 NN denotes ratio
T7194 127-129 IN denotes of
T7195 130-135 CD denotes 3×106
T7196 136-143 NN denotes cells/4
T7197 144-146 VB denotes µg
T7198 147-150 NN denotes DNA
T7199 151-154 IN denotes for
T7200 155-158 CD denotes 7–8
T7201 159-160 NN denotes h
T7202 161-163 IN denotes in
T7203 164-172 JJ denotes complete
T7204 173-179 NN denotes medium
T7205 180-181 -LRB- denotes (
T7206 181-183 CD denotes 10
T7207 183-184 NN denotes %
T7208 185-191 JJ denotes bovine
T7209 192-197 NN denotes serum
T7210 198-200 IN denotes in
T7211 201-212 NN denotes RPMI1640+15
T7212 213-224 NN denotes L-glutamine
T7213 224-225 -RRB- denotes )
T7214 226-235 VB denotes according
T7215 236-238 TO denotes to
T7216 239-242 DT denotes the
T7217 243-250 JJ denotes general
T7218 251-256 NNP denotes Amaxa
T7219 257-265 NN denotes protocol
T7220 266-269 IN denotes for
T7221 270-283 NN denotes nucleofection
T7222 285-288 DT denotes The
T7223 289-295 NN denotes medium
T7224 296-299 VB denotes was
T7225 300-312 RB denotes subsequently
T7226 313-320 VB denotes changed
T7227 321-323 TO denotes to
T7228 324-325 CD denotes 1
T7229 325-326 NN denotes %
T7230 327-331 NN denotes RPMI
T7231 332-335 IN denotes for
T7232 336-338 CD denotes 24
T7233 339-340 NN denotes h
T7234 341-347 IN denotes before
T7235 348-359 VB denotes transfected
T7236 360-365 NN denotes cells
T7237 366-370 VB denotes were
T7238 371-376 VB denotes added
T7239 377-379 TO denotes to
T7240 380-393 JJ denotes anti-CD3/CD28
T7241 394-401 VB denotes treated
T7242 402-407 NN denotes wells
T7243 408-411 CC denotes and
T7244 412-416 JJ denotes live
T7245 417-421 NN denotes cell
T7246 422-437 NN denotes videomicroscopy
T7247 438-447 VB denotes performed
R6131 T7174 T7173 arg1Of cells,HuT-78
R6132 T7174 T7175 arg1Of cells,were
R6133 T7174 T7176 arg2Of cells,transfected
R6134 T7176 T7175 arg2Of transfected,were
R6135 T7176 T7177 arg1Of transfected,with
R6136 T7179 T7180 arg1Of EP8,or
R6137 T7180 T7177 arg2Of or,with
R6138 T7180 T7178 arg1Of or,either
R6139 T7182 T7180 arg2Of vector,or
R6140 T7182 T7181 arg1Of vector,GFP
R6141 T7184 T7183 arg1Of DNA,only
R6142 T7184 T7185 arg1Of DNA,using
R6143 T7184 T7197 arg1Of DNA,µg
R6144 T7187 T7185 arg2Of R,using
R6145 T7187 T7186 arg1Of R,solution
R6146 T7187 T7188 arg1Of R,","
R6147 T7190 T7188 arg2Of V-001,","
R6148 T7190 T7189 arg1Of V-001,programme
R6149 T7190 T7191 arg1Of V-001,at
R6150 T7193 T7191 arg2Of ratio,at
R6151 T7193 T7192 arg1Of ratio,a
R6152 T7193 T7194 arg1Of ratio,of
R6153 T7196 T7194 arg2Of cells/4,of
R6154 T7196 T7195 arg1Of cells/4,3×106
R6155 T7197 T7175 modOf µg,were
R6156 T7197 T7199 arg1Of µg,for
R6157 T7197 T7202 arg1Of µg,in
R6158 T7197 T7214 arg1Of µg,according
R6159 T7198 T7197 arg2Of DNA,µg
R6160 T7201 T7199 arg2Of h,for
R6161 T7201 T7200 arg1Of h,7–8
R6162 T7204 T7202 arg2Of medium,in
R6163 T7204 T7203 arg1Of medium,complete
R6164 T7204 T7205 arg1Of medium,(
R6165 T7206 T7207 arg1Of 10,%
R6166 T7209 T7205 arg2Of serum,(
R6167 T7209 T7206 arg1Of serum,10
R6168 T7209 T7208 arg1Of serum,bovine
R6169 T7209 T7210 arg1Of serum,in
R6170 T7212 T7210 arg2Of L-glutamine,in
R6171 T7212 T7211 arg1Of L-glutamine,RPMI1640+15
R6172 T7213 T7205 arg3Of ),(
R6173 T7215 T7214 arg2Of to,according
R6174 T7219 T7215 arg2Of protocol,to
R6175 T7219 T7216 arg1Of protocol,the
R6176 T7219 T7217 arg1Of protocol,general
R6177 T7219 T7218 arg1Of protocol,Amaxa
R6178 T7219 T7220 arg1Of protocol,for
R6179 T7221 T7220 arg2Of nucleofection,for
R6180 T7223 T7222 arg1Of medium,The
R6181 T7223 T7224 arg1Of medium,was
R6182 T7223 T7226 arg2Of medium,changed
R6183 T7226 T7224 arg2Of changed,was
R6184 T7226 T7225 arg1Of changed,subsequently
R6185 T7226 T7227 arg1Of changed,to
R6186 T7226 T7231 arg1Of changed,for
R6187 T7228 T7229 arg1Of 1,%
R6188 T7230 T7227 arg2Of RPMI,to
R6189 T7230 T7228 arg1Of RPMI,1
R6190 T7233 T7232 arg1Of h,24
R6191 T7233 T7234 arg1Of h,before
R6192 T7233 T7243 arg1Of h,and
R6193 T7236 T7235 arg2Of cells,transfected
R6194 T7236 T7237 arg1Of cells,were
R6195 T7236 T7238 arg2Of cells,added
R6196 T7238 T7234 arg2Of added,before
R6197 T7238 T7237 arg2Of added,were
R6198 T7238 T7239 arg1Of added,to
R6199 T7242 T7239 arg2Of wells,to
R6200 T7242 T7240 arg1Of wells,anti-CD3/CD28
R6201 T7242 T7241 arg2Of wells,treated
R6202 T7243 T7231 arg2Of and,for
R6203 T7246 T7243 arg2Of videomicroscopy,and
R6204 T7246 T7244 arg1Of videomicroscopy,live
R6205 T7246 T7245 arg1Of videomicroscopy,cell
R6206 T7246 T7247 arg2Of videomicroscopy,performed

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T7254 0-12 NNP denotes Transfection
T7255 13-19 NNP denotes HuT-78
T7256 20-25 NNS denotes cells
T7257 26-30 VBD denotes were
T7258 31-42 VBN denotes transfected
T7259 43-47 IN denotes with
T7260 48-54 DT denotes either
T7261 55-58 CD denotes EP8
T7262 59-61 CC denotes or
T7263 62-65 NNP denotes GFP
T7264 66-72 NN denotes vector
T7265 73-77 RB denotes only
T7266 78-81 NNP denotes DNA
T7267 82-87 VBG denotes using
T7268 88-96 NN denotes solution
T7269 97-98 NN denotes R
T7270 98-99 , denotes ,
T7271 100-109 NN denotes programme
T7272 110-115 NN denotes V-001
T7273 116-118 IN denotes at
T7274 119-120 DT denotes a
T7275 121-126 NN denotes ratio
T7276 127-129 IN denotes of
T7277 130-131 CD denotes 3
T7278 131-132 CD denotes ×
T7279 132-135 CD denotes 106
T7280 136-143 CD denotes cells/4
T7281 144-146 NN denotes µg
T7282 147-150 NN denotes DNA
T7283 151-154 IN denotes for
T7284 155-156 CD denotes 7
T7285 157-158 CD denotes 8
T7286 159-160 NN denotes h
T7287 161-163 IN denotes in
T7288 164-172 JJ denotes complete
T7289 173-179 NN denotes medium
T7290 180-181 -LRB- denotes (
T7291 181-183 CD denotes 10
T7292 183-184 NN denotes %
T7293 185-191 JJ denotes bovine
T7294 192-197 NN denotes serum
T7295 198-200 IN denotes in
T7296 201-209 NNP denotes RPMI1640
T7297 209-212 CD denotes +15
T7298 213-224 NN denotes L-glutamine
T7299 224-225 -RRB- denotes )
T7300 226-235 VBG denotes according
T7301 236-238 TO denotes to
T7302 239-242 DT denotes the
T7303 243-250 JJ denotes general
T7304 251-256 NNP denotes Amaxa
T7305 257-265 NN denotes protocol
T7306 266-269 IN denotes for
T7307 270-283 NN denotes nucleofection
T7308 283-284 . denotes .
T7309 285-288 DT denotes The
T7310 289-295 NN denotes medium
T7311 296-299 VBD denotes was
T7312 300-312 RB denotes subsequently
T7313 313-320 VBN denotes changed
T7314 321-323 TO denotes to
T7315 324-325 CD denotes 1
T7316 325-326 NN denotes %
T7317 327-331 NNP denotes RPMI
T7318 332-335 IN denotes for
T7319 336-338 CD denotes 24
T7320 339-340 NN denotes h
T7321 341-347 IN denotes before
T7322 348-359 VBN denotes transfected
T7323 360-365 NNS denotes cells
T7324 366-370 VBD denotes were
T7325 371-376 VBN denotes added
T7326 377-379 TO denotes to
T7327 380-388 JJ denotes anti-CD3
T7328 388-389 NN denotes /
T7329 389-393 CD denotes CD28
T7330 394-401 VBN denotes treated
T7331 402-407 NNS denotes wells
T7332 408-411 CC denotes and
T7333 412-416 VBP denotes live
T7334 417-421 NN denotes cell
T7335 422-437 JJ denotes videomicroscopy
T7336 438-447 VBN denotes performed
T7337 447-448 . denotes .
R6247 T7294 T7286 appos serum,h
R6248 T7295 T7294 prep in,serum
R6249 T7296 T7295 pobj RPMI1640,in
R6250 T7297 T7298 nummod +15,L-glutamine
R6251 T7298 T7294 appos L-glutamine,serum
R6252 T7299 T7286 punct ),h
R6253 T7300 T7258 prep according,transfected
R6254 T7301 T7300 prep to,according
R6255 T7302 T7305 det the,protocol
R6256 T7303 T7305 amod general,protocol
R6257 T7304 T7305 compound Amaxa,protocol
R6258 T7305 T7301 pobj protocol,to
R6207 T7254 T7255 compound Transfection,HuT-78
R6208 T7255 T7256 compound HuT-78,cells
R6209 T7256 T7258 nsubjpass cells,transfected
R6210 T7257 T7258 auxpass were,transfected
R6211 T7258 T7258 ROOT transfected,transfected
R6212 T7259 T7258 prep with,transfected
R6213 T7260 T7261 det either,EP8
R6214 T7261 T7259 pobj EP8,with
R6215 T7262 T7261 cc or,EP8
R6216 T7263 T7264 compound GFP,vector
R6217 T7264 T7261 conj vector,EP8
R6218 T7265 T7266 advmod only,DNA
R6219 T7266 T7267 nsubj DNA,using
R6220 T7267 T7258 advcl using,transfected
R6221 T7268 T7269 compound solution,R
R6222 T7269 T7267 dobj R,using
R6223 T7270 T7269 punct ",",R
R6224 T7271 T7272 compound programme,V-001
R6225 T7272 T7269 appos V-001,R
R6226 T7273 T7267 prep at,using
R6227 T7274 T7275 det a,ratio
R6228 T7275 T7282 nmod ratio,DNA
R6229 T7276 T7275 prep of,ratio
R6230 T7277 T7280 nummod 3,cells/4
R6231 T7278 T7280 nummod ×,cells/4
R6232 T7279 T7280 nummod 106,cells/4
R6233 T7280 T7276 pobj cells/4,of
R6234 T7281 T7282 compound µg,DNA
R6235 T7282 T7273 pobj DNA,at
R6236 T7283 T7267 prep for,using
R6237 T7284 T7283 pobj 7,for
R6238 T7285 T7286 nummod 8,h
R6239 T7286 T7284 appos h,7
R6240 T7287 T7286 prep in,h
R6241 T7288 T7289 amod complete,medium
R6242 T7289 T7287 pobj medium,in
R6243 T7290 T7286 punct (,h
R6244 T7291 T7292 nummod 10,%
R6245 T7292 T7294 nmod %,serum
R6246 T7293 T7294 amod bovine,serum
R6259 T7306 T7305 prep for,protocol
R6260 T7307 T7306 pobj nucleofection,for
R6261 T7308 T7258 punct .,transfected
R6262 T7309 T7310 det The,medium
R6263 T7310 T7313 nsubjpass medium,changed
R6264 T7311 T7313 auxpass was,changed
R6265 T7312 T7313 advmod subsequently,changed
R6266 T7313 T7313 ROOT changed,changed
R6267 T7314 T7313 prep to,changed
R6268 T7315 T7317 nummod 1,RPMI
R6269 T7316 T7315 quantmod %,1
R6270 T7317 T7314 pobj RPMI,to
R6271 T7318 T7313 prep for,changed
R6272 T7319 T7320 nummod 24,h
R6273 T7320 T7318 pobj h,for
R6274 T7321 T7325 mark before,added
R6275 T7322 T7323 amod transfected,cells
R6276 T7323 T7325 nsubjpass cells,added
R6277 T7324 T7325 auxpass were,added
R6278 T7325 T7313 advcl added,changed
R6279 T7326 T7325 prep to,added
R6280 T7327 T7336 amod anti-CD3,performed
R6281 T7328 T7331 nummod /,wells
R6282 T7329 T7331 nummod CD28,wells
R6283 T7330 T7331 amod treated,wells
R6284 T7331 T7327 dobj wells,anti-CD3
R6285 T7332 T7327 cc and,anti-CD3
R6286 T7333 T7335 amod live,videomicroscopy
R6287 T7334 T7335 compound cell,videomicroscopy
R6288 T7335 T7327 conj videomicroscopy,anti-CD3
R6289 T7336 T7326 pobj performed,to
R6290 T7337 T7313 punct .,changed

UBERON-AE

Id Subject Object Predicate Lexical cue
T7168 192-197 http://purl.obolibrary.org/obo/UBERON_0001977 denotes serum

GO-CC

Id Subject Object Predicate Lexical cue
T7341 20-25 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7342 136-141 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7343 360-365 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T7344 417-421 http://purl.obolibrary.org/obo/GO_0005623 denotes cell

sentences

Id Subject Object Predicate Lexical cue
T7169 0-12 Sentence denotes Transfection
T7170 13-284 Sentence denotes HuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection.
T7171 285-448 Sentence denotes The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed.
T147 0-12 Sentence denotes Transfection
T148 13-284 Sentence denotes HuT-78 cells were transfected with either EP8 or GFP vector only DNA using solution R, programme V-001 at a ratio of 3×106 cells/4 µg DNA for 7–8 h in complete medium (10% bovine serum in RPMI1640+15 L-glutamine) according to the general Amaxa protocol for nucleofection.
T149 285-448 Sentence denotes The medium was subsequently changed to 1% RPMI for 24 h before transfected cells were added to anti-CD3/CD28 treated wells and live cell videomicroscopy performed.

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T7345 380-388 Protein denotes anti-CD3
T7346 389-393 Protein denotes CD28

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T7248 62-65 Q9U6Y4 denotes GFP
T7249 385-388 P20963 denotes CD3
T7250 385-388 P09693 denotes CD3
T7251 385-388 P07766 denotes CD3
T7252 385-388 P04234 denotes CD3
T7253 389-393 P10747 denotes CD28