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In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T6249","span":{"begin":17,"end":30},"obj":"http://purl.obolibrary.org/obo/GO_0051059"},{"id":"T6250","span":{"begin":23,"end":30},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T6251","span":{"begin":330,"end":337},"obj":"http://purl.obolibrary.org/obo/GO_0005488"},{"id":"T6252","span":{"begin":311,"end":319},"obj":"http://purl.obolibrary.org/obo/GO_0003823"},{"id":"T6253","span":{"begin":383,"end":391},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"NF-κB Activation\nNF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T6254","span":{"begin":311,"end":319},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T6255","span":{"begin":383,"end":391},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T6256","span":{"begin":311,"end":319},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T6257","span":{"begin":383,"end":391},"obj":"http://purl.obolibrary.org/obo/GO_0042571"}],"text":"NF-κB Activation\nNF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}
sentences
{"project":"sentences","denotations":[{"id":"T6074","span":{"begin":0,"end":16},"obj":"Sentence"},{"id":"T6075","span":{"begin":17,"end":149},"obj":"Sentence"},{"id":"T6076","span":{"begin":150,"end":260},"obj":"Sentence"},{"id":"T6077","span":{"begin":261,"end":320},"obj":"Sentence"},{"id":"T6078","span":{"begin":321,"end":425},"obj":"Sentence"},{"id":"T6079","span":{"begin":426,"end":479},"obj":"Sentence"},{"id":"T124","span":{"begin":0,"end":16},"obj":"Sentence"},{"id":"T125","span":{"begin":17,"end":149},"obj":"Sentence"},{"id":"T126","span":{"begin":150,"end":260},"obj":"Sentence"},{"id":"T127","span":{"begin":261,"end":320},"obj":"Sentence"},{"id":"T128","span":{"begin":321,"end":425},"obj":"Sentence"},{"id":"T129","span":{"begin":426,"end":479},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"NF-κB Activation\nNF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}
events-check-again
{"project":"events-check-again","denotations":[{"id":"T6260","span":{"begin":110,"end":113},"obj":"Protein"}],"text":"NF-κB Activation\nNF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T6261","span":{"begin":0,"end":5},"obj":"Protein"},{"id":"T6262","span":{"begin":6,"end":16},"obj":"Positive_regulation"},{"id":"T6263","span":{"begin":17,"end":22},"obj":"Protein"},{"id":"T6264","span":{"begin":23,"end":30},"obj":"Binding"},{"id":"T6265","span":{"begin":228,"end":259},"obj":"Protein"},{"id":"T6266","span":{"begin":302,"end":319},"obj":"Protein"},{"id":"T6267","span":{"begin":368,"end":391},"obj":"Protein"},{"id":"T6268","span":{"begin":330,"end":337},"obj":"Binding"}],"relations":[{"id":"R5386","pred":"themeOf","subj":"T6261","obj":"T6262"},{"id":"R5387","pred":"themeOf","subj":"T6263","obj":"T6264"},{"id":"R5388","pred":"themeOf","subj":"T6267","obj":"T6268"}],"text":"NF-κB Activation\nNF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T6073","span":{"begin":110,"end":113},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"NF-κB Activation\nNF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T6157","span":{"begin":110,"end":113},"obj":"Q04206"},{"id":"T6158","span":{"begin":110,"end":113},"obj":"P21579"},{"id":"T6159","span":{"begin":307,"end":310},"obj":"Q04206"},{"id":"T6160","span":{"begin":307,"end":310},"obj":"P21579"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"NF-κB Activation\nNF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}
test2
{"project":"test2","denotations":[{"id":"T6072","span":{"begin":110,"end":113},"obj":"Protein"}],"text":"NF-κB Activation\nNF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. Plates were washed before addition of an anti-p65 antibody. Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. The intensity of the reaction was measured at 450 nm."}