PMC:2674207 / 18033-18512
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6080 | 0-5 | NN | denotes | NF-κB |
T6081 | 6-16 | NN | denotes | Activation |
T6082 | 17-22 | NN | denotes | NF-κB |
T6083 | 23-30 | NN | denotes | binding |
T6084 | 31-39 | NN | denotes | activity |
T6085 | 40-42 | IN | denotes | in |
T6086 | 43-50 | JJ | denotes | nuclear |
T6087 | 51-59 | NN | denotes | extracts |
T6088 | 60-63 | VB | denotes | was |
T6089 | 64-74 | VB | denotes | determined |
T6090 | 75-80 | VB | denotes | using |
T6091 | 81-83 | DT | denotes | an |
T6092 | 84-95 | JJ | denotes | ELISA-based |
T6093 | 96-99 | NN | denotes | kit |
T6094 | 100-101 | -LRB- | denotes | ( |
T6095 | 101-109 | NN | denotes | Trans-AM |
T6096 | 110-113 | NN | denotes | p65 |
T6097 | 113-114 | -COMMA- | denotes | , |
T6098 | 115-121 | NNP | denotes | Active |
T6099 | 122-127 | NNP | denotes | Motif |
T6100 | 127-128 | -COMMA- | denotes | , |
T6101 | 129-138 | NNP | denotes | Rixensart |
T6102 | 138-139 | -COMMA- | denotes | , |
T6103 | 140-147 | NNP | denotes | Belgium |
T6104 | 147-148 | -RRB- | denotes | ) |
T6105 | 150-152 | IN | denotes | In |
T6106 | 153-158 | NN | denotes | brief |
T6107 | 158-159 | -COMMA- | denotes | , |
T6108 | 160-161 | CD | denotes | 5 |
T6109 | 162-164 | NN | denotes | µg |
T6110 | 165-167 | IN | denotes | of |
T6111 | 168-175 | JJ | denotes | nuclear |
T6112 | 176-184 | NN | denotes | extracts |
T6113 | 185-189 | VB | denotes | were |
T6114 | 190-199 | VB | denotes | incubated |
T6115 | 200-204 | IN | denotes | with |
T6116 | 205-206 | DT | denotes | a |
T6117 | 207-212 | NN | denotes | plate |
T6118 | 213-219 | VB | denotes | coated |
T6119 | 220-224 | IN | denotes | with |
T6120 | 225-227 | DT | denotes | an |
T6121 | 228-233 | NN | denotes | NF-κB |
T6122 | 234-243 | NN | denotes | consensus |
T6123 | 244-259 | NN | denotes | oligonucleotide |
T6124 | 261-267 | NN | denotes | Plates |
T6125 | 268-272 | VB | denotes | were |
T6126 | 273-279 | VB | denotes | washed |
T6127 | 280-286 | IN | denotes | before |
T6128 | 287-295 | NN | denotes | addition |
T6129 | 296-298 | IN | denotes | of |
T6130 | 299-301 | DT | denotes | an |
T6131 | 302-310 | JJ | denotes | anti-p65 |
T6132 | 311-319 | NN | denotes | antibody |
T6133 | 321-329 | NN | denotes | Antibody |
T6134 | 330-337 | NN | denotes | binding |
T6135 | 338-341 | VB | denotes | was |
T6136 | 342-350 | VB | denotes | detected |
T6137 | 351-355 | IN | denotes | with |
T6138 | 356-357 | DT | denotes | a |
T6139 | 358-367 | JJ | denotes | secondary |
T6140 | 368-382 | JJ | denotes | HRP-conjugated |
T6141 | 383-391 | NN | denotes | antibody |
T6142 | 392-395 | CC | denotes | and |
T6143 | 396-405 | VB | denotes | developed |
T6144 | 406-410 | IN | denotes | with |
T6145 | 411-414 | NN | denotes | TMB |
T6146 | 415-424 | NN | denotes | substrate |
T6147 | 426-429 | DT | denotes | The |
T6148 | 430-439 | NN | denotes | intensity |
T6149 | 440-442 | IN | denotes | of |
T6150 | 443-446 | DT | denotes | the |
T6151 | 447-455 | NN | denotes | reaction |
T6152 | 456-459 | VB | denotes | was |
T6153 | 460-468 | VB | denotes | measured |
T6154 | 469-471 | IN | denotes | at |
T6155 | 472-475 | CD | denotes | 450 |
T6156 | 476-478 | NN | denotes | nm |
R5227 | T6081 | T6080 | arg1Of | Activation,NF-κB |
R5228 | T6084 | T6082 | arg1Of | activity,NF-κB |
R5229 | T6084 | T6083 | arg1Of | activity,binding |
R5230 | T6084 | T6085 | arg1Of | activity,in |
R5231 | T6084 | T6088 | arg1Of | activity,was |
R5232 | T6084 | T6089 | arg2Of | activity,determined |
R5233 | T6087 | T6085 | arg2Of | extracts,in |
R5234 | T6087 | T6086 | arg1Of | extracts,nuclear |
R5235 | T6089 | T6088 | arg2Of | determined,was |
R5236 | T6090 | T6089 | arg3Of | using,determined |
R5237 | T6093 | T6090 | arg2Of | kit,using |
R5238 | T6093 | T6091 | arg1Of | kit,an |
R5239 | T6093 | T6092 | arg1Of | kit,ELISA-based |
R5240 | T6093 | T6094 | arg1Of | kit,( |
R5241 | T6096 | T6095 | arg1Of | p65,Trans-AM |
R5242 | T6096 | T6097 | arg1Of | p65,"," |
R5243 | T6096 | T6100 | arg1Of | p65,"," |
R5244 | T6099 | T6097 | arg2Of | Motif,"," |
R5245 | T6099 | T6098 | arg1Of | Motif,Active |
R5246 | T6100 | T6094 | arg2Of | ",",( |
R5247 | T6101 | T6100 | arg2Of | Rixensart,"," |
R5248 | T6101 | T6102 | arg1Of | Rixensart,"," |
R5249 | T6103 | T6102 | arg2Of | Belgium,"," |
R5250 | T6104 | T6094 | arg3Of | ),( |
R5251 | T6106 | T6105 | arg2Of | brief,In |
R5252 | T6109 | T6108 | arg1Of | µg,5 |
R5253 | T6109 | T6110 | arg1Of | µg,of |
R5254 | T6109 | T6113 | arg1Of | µg,were |
R5255 | T6109 | T6114 | arg2Of | µg,incubated |
R5256 | T6112 | T6110 | arg2Of | extracts,of |
R5257 | T6112 | T6111 | arg1Of | extracts,nuclear |
R5258 | T6114 | T6105 | arg1Of | incubated,In |
R5259 | T6114 | T6107 | arg1Of | incubated,"," |
R5260 | T6114 | T6113 | arg2Of | incubated,were |
R5261 | T6114 | T6115 | arg1Of | incubated,with |
R5262 | T6117 | T6115 | arg2Of | plate,with |
R5263 | T6117 | T6116 | arg1Of | plate,a |
R5264 | T6117 | T6118 | arg2Of | plate,coated |
R5265 | T6118 | T6119 | arg1Of | coated,with |
R5266 | T6123 | T6119 | arg2Of | oligonucleotide,with |
R5267 | T6123 | T6120 | arg1Of | oligonucleotide,an |
R5268 | T6123 | T6121 | arg1Of | oligonucleotide,NF-κB |
R5269 | T6123 | T6122 | arg1Of | oligonucleotide,consensus |
R5270 | T6124 | T6125 | arg1Of | Plates,were |
R5271 | T6124 | T6126 | arg2Of | Plates,washed |
R5272 | T6126 | T6125 | arg2Of | washed,were |
R5273 | T6126 | T6127 | arg1Of | washed,before |
R5274 | T6128 | T6127 | arg2Of | addition,before |
R5275 | T6128 | T6129 | arg1Of | addition,of |
R5276 | T6132 | T6129 | arg2Of | antibody,of |
R5277 | T6132 | T6130 | arg1Of | antibody,an |
R5278 | T6132 | T6131 | arg1Of | antibody,anti-p65 |
R5279 | T6134 | T6133 | arg1Of | binding,Antibody |
R5280 | T6134 | T6135 | arg1Of | binding,was |
R5281 | T6134 | T6136 | arg2Of | binding,detected |
R5282 | T6134 | T6143 | arg2Of | binding,developed |
R5283 | T6136 | T6137 | arg1Of | detected,with |
R5284 | T6136 | T6142 | arg1Of | detected,and |
R5285 | T6141 | T6137 | arg2Of | antibody,with |
R5286 | T6141 | T6138 | arg1Of | antibody,a |
R5287 | T6141 | T6139 | arg1Of | antibody,secondary |
R5288 | T6141 | T6140 | arg1Of | antibody,HRP-conjugated |
R5289 | T6142 | T6135 | arg2Of | and,was |
R5290 | T6143 | T6142 | arg2Of | developed,and |
R5291 | T6143 | T6144 | arg1Of | developed,with |
R5292 | T6146 | T6144 | arg2Of | substrate,with |
R5293 | T6146 | T6145 | arg1Of | substrate,TMB |
R5294 | T6148 | T6147 | arg1Of | intensity,The |
R5295 | T6148 | T6149 | arg1Of | intensity,of |
R5296 | T6148 | T6152 | arg1Of | intensity,was |
R5297 | T6148 | T6153 | arg2Of | intensity,measured |
R5298 | T6151 | T6149 | arg2Of | reaction,of |
R5299 | T6151 | T6150 | arg1Of | reaction,the |
R5300 | T6153 | T6152 | arg2Of | measured,was |
R5301 | T6153 | T6154 | arg1Of | measured,at |
R5302 | T6156 | T6154 | arg2Of | nm,at |
R5303 | T6156 | T6155 | arg1Of | nm,450 |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6161 | 0-5 | NNP | denotes | NF-κB |
T6162 | 6-16 | NNP | denotes | Activation |
T6163 | 17-22 | NNP | denotes | NF-κB |
T6164 | 23-30 | JJ | denotes | binding |
T6165 | 31-39 | NN | denotes | activity |
T6166 | 40-42 | IN | denotes | in |
T6167 | 43-50 | JJ | denotes | nuclear |
T6168 | 51-59 | NNS | denotes | extracts |
T6169 | 60-63 | VBD | denotes | was |
T6170 | 64-74 | VBN | denotes | determined |
T6171 | 75-80 | VBG | denotes | using |
T6172 | 81-83 | DT | denotes | an |
T6173 | 84-95 | JJ | denotes | ELISA-based |
T6174 | 96-99 | NN | denotes | kit |
T6175 | 100-101 | -LRB- | denotes | ( |
T6176 | 101-109 | JJ | denotes | Trans-AM |
T6177 | 110-113 | NNS | denotes | p65 |
T6178 | 113-114 | , | denotes | , |
T6179 | 115-121 | NNP | denotes | Active |
T6180 | 122-127 | NNP | denotes | Motif |
T6181 | 127-128 | , | denotes | , |
T6182 | 129-138 | NNP | denotes | Rixensart |
T6183 | 138-139 | , | denotes | , |
T6184 | 140-147 | NNP | denotes | Belgium |
T6185 | 147-148 | -RRB- | denotes | ) |
T6186 | 148-149 | . | denotes | . |
T6187 | 150-152 | IN | denotes | In |
T6188 | 153-158 | NN | denotes | brief |
T6189 | 158-159 | , | denotes | , |
T6190 | 160-161 | CD | denotes | 5 |
T6191 | 162-164 | NN | denotes | µg |
T6192 | 165-167 | IN | denotes | of |
T6193 | 168-175 | JJ | denotes | nuclear |
T6194 | 176-184 | NNS | denotes | extracts |
T6195 | 185-189 | VBD | denotes | were |
T6196 | 190-199 | VBN | denotes | incubated |
T6197 | 200-204 | IN | denotes | with |
T6198 | 205-206 | DT | denotes | a |
T6199 | 207-212 | NN | denotes | plate |
T6200 | 213-219 | VBN | denotes | coated |
T6201 | 220-224 | IN | denotes | with |
T6202 | 225-227 | DT | denotes | an |
T6203 | 228-233 | JJ | denotes | NF-κB |
T6204 | 234-243 | NN | denotes | consensus |
T6205 | 244-259 | NN | denotes | oligonucleotide |
T6206 | 259-260 | . | denotes | . |
T6207 | 261-267 | NNS | denotes | Plates |
T6208 | 268-272 | VBD | denotes | were |
T6209 | 273-279 | VBN | denotes | washed |
T6210 | 280-286 | IN | denotes | before |
T6211 | 287-295 | NN | denotes | addition |
T6212 | 296-298 | IN | denotes | of |
T6213 | 299-301 | DT | denotes | an |
T6214 | 302-310 | JJ | denotes | anti-p65 |
T6215 | 311-319 | NN | denotes | antibody |
T6216 | 319-320 | . | denotes | . |
T6217 | 321-329 | NN | denotes | Antibody |
T6218 | 330-337 | JJ | denotes | binding |
T6219 | 338-341 | VBD | denotes | was |
T6220 | 342-350 | VBN | denotes | detected |
T6221 | 351-355 | IN | denotes | with |
T6222 | 356-357 | DT | denotes | a |
T6223 | 358-367 | JJ | denotes | secondary |
T6224 | 368-382 | JJ | denotes | HRP-conjugated |
T6225 | 383-391 | NN | denotes | antibody |
T6226 | 392-395 | CC | denotes | and |
T6227 | 396-405 | VBN | denotes | developed |
T6228 | 406-410 | IN | denotes | with |
T6229 | 411-414 | NNP | denotes | TMB |
T6230 | 415-424 | NN | denotes | substrate |
T6231 | 424-425 | . | denotes | . |
T6232 | 426-429 | DT | denotes | The |
T6233 | 430-439 | NN | denotes | intensity |
T6234 | 440-442 | IN | denotes | of |
T6235 | 443-446 | DT | denotes | the |
T6236 | 447-455 | NN | denotes | reaction |
T6237 | 456-459 | VBD | denotes | was |
T6238 | 460-468 | VBN | denotes | measured |
T6239 | 469-471 | IN | denotes | at |
T6240 | 472-475 | CD | denotes | 450 |
T6241 | 476-478 | NN | denotes | nm |
T6242 | 478-479 | . | denotes | . |
R5304 | T6161 | T6163 | compound | NF-κB,NF-κB |
R5305 | T6162 | T6163 | compound | Activation,NF-κB |
R5306 | T6163 | T6170 | nsubjpass | NF-κB,determined |
R5307 | T6164 | T6165 | amod | binding,activity |
R5308 | T6165 | T6163 | dobj | activity,NF-κB |
R5309 | T6166 | T6165 | prep | in,activity |
R5310 | T6167 | T6168 | amod | nuclear,extracts |
R5311 | T6168 | T6166 | pobj | extracts,in |
R5312 | T6169 | T6170 | auxpass | was,determined |
R5313 | T6170 | T6170 | ROOT | determined,determined |
R5342 | T6199 | T6197 | pobj | plate,with |
R5343 | T6200 | T6199 | acl | coated,plate |
R5344 | T6201 | T6200 | prep | with,coated |
R5345 | T6202 | T6204 | det | an,consensus |
R5346 | T6203 | T6204 | amod | NF-κB,consensus |
R5347 | T6204 | T6205 | compound | consensus,oligonucleotide |
R5348 | T6205 | T6201 | pobj | oligonucleotide,with |
R5349 | T6206 | T6196 | punct | .,incubated |
R5350 | T6207 | T6209 | nsubjpass | Plates,washed |
R5351 | T6208 | T6209 | auxpass | were,washed |
R5352 | T6209 | T6209 | ROOT | washed,washed |
R5353 | T6210 | T6209 | prep | before,washed |
R5354 | T6211 | T6210 | pobj | addition,before |
R5355 | T6212 | T6211 | prep | of,addition |
R5356 | T6213 | T6215 | det | an,antibody |
R5357 | T6214 | T6215 | amod | anti-p65,antibody |
R5358 | T6215 | T6212 | pobj | antibody,of |
R5359 | T6216 | T6209 | punct | .,washed |
R5360 | T6217 | T6218 | compound | Antibody,binding |
R5361 | T6218 | T6220 | nsubjpass | binding,detected |
R5362 | T6219 | T6220 | auxpass | was,detected |
R5363 | T6220 | T6220 | ROOT | detected,detected |
R5364 | T6221 | T6220 | prep | with,detected |
R5365 | T6222 | T6225 | det | a,antibody |
R5366 | T6223 | T6225 | amod | secondary,antibody |
R5367 | T6224 | T6225 | amod | HRP-conjugated,antibody |
R5368 | T6225 | T6221 | pobj | antibody,with |
R5369 | T6226 | T6220 | cc | and,detected |
R5370 | T6227 | T6220 | conj | developed,detected |
R5371 | T6228 | T6227 | prep | with,developed |
R5372 | T6229 | T6230 | compound | TMB,substrate |
R5373 | T6230 | T6228 | pobj | substrate,with |
R5374 | T6231 | T6220 | punct | .,detected |
R5375 | T6232 | T6233 | det | The,intensity |
R5376 | T6233 | T6238 | nsubjpass | intensity,measured |
R5377 | T6234 | T6233 | prep | of,intensity |
R5378 | T6235 | T6236 | det | the,reaction |
R5379 | T6236 | T6234 | pobj | reaction,of |
R5380 | T6237 | T6238 | auxpass | was,measured |
R5381 | T6238 | T6238 | ROOT | measured,measured |
R5382 | T6239 | T6238 | prep | at,measured |
R5383 | T6240 | T6241 | nummod | 450,nm |
R5384 | T6241 | T6239 | pobj | nm,at |
R5385 | T6242 | T6238 | punct | .,measured |
R5314 | T6171 | T6170 | advcl | using,determined |
R5315 | T6172 | T6174 | det | an,kit |
R5316 | T6173 | T6174 | amod | ELISA-based,kit |
R5317 | T6174 | T6171 | dobj | kit,using |
R5318 | T6175 | T6174 | punct | (,kit |
R5319 | T6176 | T6177 | amod | Trans-AM,p65 |
R5320 | T6177 | T6174 | appos | p65,kit |
R5321 | T6178 | T6177 | punct | ",",p65 |
R5322 | T6179 | T6180 | compound | Active,Motif |
R5323 | T6180 | T6177 | appos | Motif,p65 |
R5324 | T6181 | T6180 | punct | ",",Motif |
R5325 | T6182 | T6180 | conj | Rixensart,Motif |
R5326 | T6183 | T6182 | punct | ",",Rixensart |
R5327 | T6184 | T6182 | conj | Belgium,Rixensart |
R5328 | T6185 | T6174 | punct | ),kit |
R5329 | T6186 | T6170 | punct | .,determined |
R5330 | T6187 | T6196 | prep | In,incubated |
R5331 | T6188 | T6187 | pobj | brief,In |
R5332 | T6189 | T6196 | punct | ",",incubated |
R5333 | T6190 | T6191 | nummod | 5,µg |
R5334 | T6191 | T6196 | nsubjpass | µg,incubated |
R5335 | T6192 | T6191 | prep | of,µg |
R5336 | T6193 | T6194 | amod | nuclear,extracts |
R5337 | T6194 | T6192 | pobj | extracts,of |
R5338 | T6195 | T6196 | auxpass | were,incubated |
R5339 | T6196 | T6196 | ROOT | incubated,incubated |
R5340 | T6197 | T6196 | prep | with,incubated |
R5341 | T6198 | T6199 | det | a,plate |
GO-MF
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6249 | 17-30 | http://purl.obolibrary.org/obo/GO_0051059 | denotes | NF-κB binding |
T6250 | 23-30 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | binding |
T6251 | 330-337 | http://purl.obolibrary.org/obo/GO_0005488 | denotes | binding |
T6252 | 311-319 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
T6253 | 383-391 | http://purl.obolibrary.org/obo/GO_0003823 | denotes | antibody |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6254 | 311-319 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
T6255 | 383-391 | http://purl.obolibrary.org/obo/GO_0019815 | denotes | antibody |
T6256 | 311-319 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
T6257 | 383-391 | http://purl.obolibrary.org/obo/GO_0042571 | denotes | antibody |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6074 | 0-16 | Sentence | denotes | NF-κB Activation |
T6075 | 17-149 | Sentence | denotes | NF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). |
T6076 | 150-260 | Sentence | denotes | In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. |
T6077 | 261-320 | Sentence | denotes | Plates were washed before addition of an anti-p65 antibody. |
T6078 | 321-425 | Sentence | denotes | Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. |
T6079 | 426-479 | Sentence | denotes | The intensity of the reaction was measured at 450 nm. |
T124 | 0-16 | Sentence | denotes | NF-κB Activation |
T125 | 17-149 | Sentence | denotes | NF-κB binding activity in nuclear extracts was determined using an ELISA-based kit (Trans-AM p65, Active Motif, Rixensart, Belgium). |
T126 | 150-260 | Sentence | denotes | In brief, 5 µg of nuclear extracts were incubated with a plate coated with an NF-κB consensus oligonucleotide. |
T127 | 261-320 | Sentence | denotes | Plates were washed before addition of an anti-p65 antibody. |
T128 | 321-425 | Sentence | denotes | Antibody binding was detected with a secondary HRP-conjugated antibody and developed with TMB substrate. |
T129 | 426-479 | Sentence | denotes | The intensity of the reaction was measured at 450 nm. |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6260 | 110-113 | Protein | denotes | p65 |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6261 | 0-5 | Protein | denotes | NF-κB |
T6262 | 6-16 | Positive_regulation | denotes | Activation |
T6263 | 17-22 | Protein | denotes | NF-κB |
T6264 | 23-30 | Binding | denotes | binding |
T6265 | 228-259 | Protein | denotes | NF-κB consensus oligonucleotide |
T6266 | 302-319 | Protein | denotes | anti-p65 antibody |
T6267 | 368-391 | Protein | denotes | HRP-conjugated antibody |
T6268 | 330-337 | Binding | denotes | binding |
R5386 | T6261 | T6262 | themeOf | NF-κB,Activation |
R5387 | T6263 | T6264 | themeOf | NF-κB,binding |
R5388 | T6267 | T6268 | themeOf | HRP-conjugated antibody,binding |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6073 | 110-113 | Protein | denotes | p65 |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6157 | 110-113 | Q04206 | denotes | p65 |
T6158 | 110-113 | P21579 | denotes | p65 |
T6159 | 307-310 | Q04206 | denotes | p65 |
T6160 | 307-310 | P21579 | denotes | p65 |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T6072 | 110-113 | Protein | denotes | p65 |