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PMC:2674207 / 16054-16677 JSONTXT

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2_test

Id Subject Object Predicate Lexical cue
19436703-17277157-86274490 104-106 17277157 denotes 12
T57036 104-106 17277157 denotes 12

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T5206 0-9 NN denotes Chromatin
T5207 10-29 NN denotes Immunoprecipitation
T5208 30-39 NN denotes Chromatin
T5209 40-59 NN denotes immunoprecipitation
T5210 60-61 -LRB- denotes (
T5211 61-63 NN denotes IP
T5212 63-64 -RRB- denotes )
T5213 65-68 VB denotes was
T5214 69-78 VB denotes performed
T5215 79-81 IN denotes as
T5216 82-92 RB denotes previously
T5217 93-102 VB denotes described
T5218 103-104 -LRB- denotes [
T5219 104-106 CD denotes 12
T5220 106-107 -RRB- denotes ]
T5221 108-110 IN denotes in
T5222 111-117 JJ denotes HuT-78
T5223 118-125 NN denotes T-cells
T5224 126-130 IN denotes with
T5225 131-132 CD denotes 2
T5226 133-135 NN denotes µg
T5227 136-138 IN denotes of
T5228 139-150 NN denotes anti-GATA-3
T5229 151-152 -LRB- denotes (
T5230 152-157 NNP denotes Santa
T5231 158-162 NNP denotes Cruz
T5232 163-176 NNP denotes Biotechnology
T5233 176-177 -RRB- denotes )
T5234 177-178 -COMMA- denotes ,
T5235 179-181 CC denotes or
T5236 182-190 JJ denotes isotypic
T5237 191-205 NN denotes immunoglobulin
T5238 206-207 NN denotes G
T5239 208-210 IN denotes as
T5240 211-212 DT denotes a
T5241 213-225 JJ denotes non-specific
T5242 226-233 NN denotes control
T5243 234-235 -LRB- denotes (
T5244 235-240 NNP denotes Santa
T5245 241-245 NNP denotes Cruz
T5246 246-259 NNP denotes Biotechnology
T5247 259-260 -RRB- denotes )
T5248 261-270 RB denotes overnight
T5249 271-273 IN denotes at
T5250 274-278 NNP denotes 4°C.
T5251 279-287 NNP denotes Promoter
T5252 288-297 NN denotes sequences
T5253 298-302 VB denotes were
T5254 303-311 VB denotes detected
T5255 312-316 IN denotes with
T5256 317-320 NN denotes PCR
T5257 321-328 NN denotes primers
T5258 329-332 IN denotes for
T5259 333-336 DT denotes the
T5260 337-341 NN denotes IL-5
T5261 342-350 NN denotes promoter
T5262 351-352 -LRB- denotes (
T5263 352-356 CD denotes −445
T5264 357-359 TO denotes to
T5265 360-362 CD denotes +4
T5266 362-363 -RRB- denotes )
T5267 363-364 -COLON- denotes :
T5268 365-372 JJ denotes forward
T5269 373-400 NN denotes 5′-TTAATCTAGCCACAGTCATAG-3′
T5270 401-404 CC denotes and
T5271 405-412 VB denotes reverse
T5272 412-413 -COLON- denotes :
T5273 414-441 NN denotes 5′-TCATGGCTCTGAAACGTTCTG-3′
T5274 443-446 NN denotes PCR
T5275 447-450 VB denotes was
T5276 451-460 VB denotes performed
T5277 461-466 VB denotes using
T5278 467-468 DT denotes a
T5279 469-475 NNP denotes Hybaid
T5280 476-484 NNP denotes Omnigene
T5281 485-492 JJ denotes thermal
T5282 493-499 NN denotes cycler
T5283 500-501 -LRB- denotes (
T5284 501-507 NNP denotes Hybaid
T5285 507-508 -COMMA- denotes ,
T5286 509-516 NNP denotes Ashford
T5287 516-517 -COMMA- denotes ,
T5288 518-520 NNP denotes UK
T5289 520-521 -RRB- denotes )
T5290 522-526 IN denotes with
T5291 527-534 VB denotes cycling
T5292 535-545 NN denotes parameters
T5293 546-548 IN denotes of
T5294 549-553 NN denotes 72°C
T5295 554-557 IN denotes for
T5296 558-560 CD denotes 10
T5297 561-564 NN denotes min
T5298 564-565 -COMMA- denotes ,
T5301 576-578 IN denotes at
T5302 579-583 NN denotes 94°C
T5303 584-587 IN denotes for
T5304 588-590 CD denotes 45
T5305 591-592 NN denotes s
T5306 592-593 -COMMA- denotes ,
T5307 594-598 NN denotes 52°C
T5308 599-602 IN denotes for
T5309 603-605 CD denotes 45
T5310 606-607 NN denotes s
T5311 607-608 -COMMA- denotes ,
T5312 609-613 NN denotes 72°C
T5313 614-617 IN denotes for
T5314 618-620 CD denotes 45
T5315 621-622 NN denotes s
T5299 566-568 CD denotes 35
T5300 569-575 NN denotes cycles
R4483 T5207 T5206 arg1Of Immunoprecipitation,Chromatin
R4484 T5209 T5208 arg1Of immunoprecipitation,Chromatin
R4485 T5209 T5210 arg1Of immunoprecipitation,(
R4486 T5209 T5213 arg1Of immunoprecipitation,was
R4487 T5209 T5214 arg2Of immunoprecipitation,performed
R4488 T5211 T5210 arg2Of IP,(
R4489 T5212 T5210 arg3Of ),(
R4490 T5214 T5213 arg2Of performed,was
R4491 T5214 T5215 arg1Of performed,as
R4492 T5214 T5239 arg1Of performed,as
R4493 T5214 T5248 arg1Of performed,overnight
R4494 T5217 T5216 arg1Of described,previously
R4495 T5219 T5215 arg2Of 12,as
R4496 T5219 T5217 arg1Of 12,described
R4497 T5219 T5218 arg2Of 12,[
R4498 T5219 T5221 arg1Of 12,in
R4499 T5219 T5224 arg1Of 12,with
R4500 T5220 T5218 arg3Of ],[
R4501 T5223 T5221 arg2Of T-cells,in
R4502 T5223 T5222 arg1Of T-cells,HuT-78
R4503 T5226 T5224 arg2Of µg,with
R4504 T5226 T5225 arg1Of µg,2
R4505 T5226 T5227 arg1Of µg,of
R4506 T5228 T5229 arg1Of anti-GATA-3,(
R4507 T5228 T5235 arg1Of anti-GATA-3,or
R4508 T5232 T5229 arg2Of Biotechnology,(
R4509 T5232 T5230 arg1Of Biotechnology,Santa
R4510 T5232 T5231 arg1Of Biotechnology,Cruz
R4511 T5233 T5229 arg3Of ),(
R4512 T5235 T5227 arg2Of or,of
R4513 T5235 T5234 arg1Of or,","
R4514 T5238 T5235 arg2Of G,or
R4515 T5238 T5236 arg1Of G,isotypic
R4516 T5238 T5237 arg1Of G,immunoglobulin
R4517 T5242 T5239 arg2Of control,as
R4518 T5242 T5240 arg1Of control,a
R4519 T5242 T5241 arg1Of control,non-specific
R4520 T5242 T5243 arg1Of control,(
R4521 T5246 T5243 arg2Of Biotechnology,(
R4522 T5246 T5244 arg1Of Biotechnology,Santa
R4523 T5246 T5245 arg1Of Biotechnology,Cruz
R4524 T5247 T5243 arg3Of ),(
R4525 T5251 T5250 arg1Of Promoter,4°C.
R4526 T5252 T5251 arg1Of sequences,Promoter
R4527 T5252 T5253 arg1Of sequences,were
R4528 T5252 T5254 arg2Of sequences,detected
R4529 T5252 T5271 arg1Of sequences,reverse
R4530 T5254 T5253 arg2Of detected,were
R4531 T5254 T5255 arg1Of detected,with
R4532 T5254 T5270 arg1Of detected,and
R4533 T5257 T5255 arg2Of primers,with
R4534 T5257 T5256 arg1Of primers,PCR
R4535 T5257 T5258 arg1Of primers,for
R4536 T5261 T5259 arg1Of promoter,the
R4537 T5261 T5260 arg1Of promoter,IL-5
R4538 T5261 T5262 arg1Of promoter,(
R4539 T5261 T5267 arg1Of promoter,:
R4540 T5265 T5262 arg2Of +4,(
R4541 T5265 T5263 arg1Of +4,−445
R4542 T5265 T5264 arg1Of +4,to
R4543 T5266 T5262 arg3Of ),(
R4544 T5269 T5258 arg2Of 5′-TTAATCTAGCCACAGTCATAG-3′,for
R4545 T5269 T5261 arg1Of 5′-TTAATCTAGCCACAGTCATAG-3′,promoter
R4546 T5269 T5268 arg1Of 5′-TTAATCTAGCCACAGTCATAG-3′,forward
R4547 T5270 T5213 modOf and,was
R4548 T5270 T5249 arg1Of and,at
R4549 T5270 T5272 arg1Of and,:
R4550 T5271 T5270 arg2Of reverse,and
R4551 T5274 T5275 arg1Of PCR,was
R4552 T5274 T5276 arg2Of PCR,performed
R4553 T5276 T5275 arg2Of performed,was
R4554 T5277 T5276 arg3Of using,performed
R4555 T5280 T5279 arg1Of Omnigene,Hybaid
R4556 T5282 T5277 arg2Of cycler,using
R4557 T5282 T5278 arg1Of cycler,a
R4558 T5282 T5280 arg1Of cycler,Omnigene
R4559 T5282 T5281 arg1Of cycler,thermal
R4560 T5282 T5283 arg1Of cycler,(
R4561 T5282 T5290 arg1Of cycler,with
R4562 T5284 T5285 arg1Of Hybaid,","
R4563 T5285 T5283 arg2Of ",",(
R4564 T5285 T5287 arg1Of ",",","
R4565 T5286 T5285 arg2Of Ashford,","
R4566 T5288 T5287 arg2Of UK,","
R4567 T5289 T5283 arg3Of ),(
R4568 T5292 T5290 arg2Of parameters,with
R4569 T5292 T5291 arg1Of parameters,cycling
R4570 T5292 T5293 arg1Of parameters,of
R4571 T5292 T5301 arg1Of parameters,at
R4572 T5294 T5295 arg1Of 72°C,for
R4573 T5294 T5298 arg1Of 72°C,","
R4574 T5297 T5295 arg2Of min,for
R4575 T5297 T5296 arg1Of min,10
R4576 T5298 T5293 arg2Of ",",of
R4577 T5300 T5298 arg2Of cycles,","
R4578 T5300 T5299 arg1Of cycles,35
R4579 T5302 T5301 arg2Of 94°C,at
R4580 T5302 T5303 arg1Of 94°C,for
R4581 T5305 T5304 arg1Of s,45
R4582 T5305 T5306 arg1Of s,","
R4583 T5306 T5311 arg1Of ",",","
R4584 T5307 T5306 arg2Of 52°C,","
R4585 T5307 T5308 arg1Of 52°C,for
R4586 T5310 T5308 arg2Of s,for
R4587 T5310 T5309 arg1Of s,45
R4588 T5311 T5303 arg2Of ",",for
R4589 T5311 T5313 arg1Of ",",for
R4590 T5312 T5311 arg2Of 72°C,","
R4591 T5315 T5313 arg2Of s,for
R4592 T5315 T5314 arg1Of s,45

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T5318 0-9 NNP denotes Chromatin
T5319 10-29 NNP denotes Immunoprecipitation
T5320 30-39 NNP denotes Chromatin
T5321 40-59 NN denotes immunoprecipitation
T5322 60-61 -LRB- denotes (
T5323 61-63 NNP denotes IP
T5324 63-64 -RRB- denotes )
T5325 65-68 VBD denotes was
T5326 69-78 VBN denotes performed
T5327 79-81 IN denotes as
T5328 82-92 RB denotes previously
T5329 93-102 VBN denotes described
T5330 103-104 NNP denotes [
T5331 104-106 CD denotes 12
T5332 106-107 NNP denotes ]
T5333 108-110 IN denotes in
T5334 111-117 NNP denotes HuT-78
T5335 118-125 NNS denotes T-cells
T5336 126-130 IN denotes with
T5337 131-132 CD denotes 2
T5338 133-135 NN denotes µg
T5339 136-138 IN denotes of
T5340 139-150 JJ denotes anti-GATA-3
T5341 151-152 -LRB- denotes (
T5342 152-157 NNP denotes Santa
T5343 158-162 NNP denotes Cruz
T5344 163-176 NNP denotes Biotechnology
T5345 176-177 -RRB- denotes )
T5346 177-178 , denotes ,
T5347 179-181 CC denotes or
T5348 182-190 JJ denotes isotypic
T5349 191-205 NN denotes immunoglobulin
T5350 206-207 NNP denotes G
T5351 208-210 IN denotes as
T5352 211-212 DT denotes a
T5353 213-225 JJ denotes non-specific
T5354 226-233 NN denotes control
T5355 234-235 -LRB- denotes (
T5356 235-240 NNP denotes Santa
T5357 241-245 NNP denotes Cruz
T5358 246-259 NNP denotes Biotechnology
T5359 259-260 -RRB- denotes )
T5360 261-270 RB denotes overnight
T5361 271-273 IN denotes at
T5362 274-275 CD denotes 4
T5363 275-276 CD denotes °
T5364 276-278 NNP denotes C.
T5365 279-287 NNP denotes Promoter
T5366 288-297 NNS denotes sequences
T5367 298-302 VBD denotes were
T5368 303-311 VBN denotes detected
T5369 312-316 IN denotes with
T5370 317-320 NNP denotes PCR
T5371 321-328 NNS denotes primers
T5372 329-332 IN denotes for
T5373 333-336 DT denotes the
T5374 337-341 NN denotes IL-5
T5375 342-350 NN denotes promoter
T5376 351-352 -LRB- denotes (
T5377 352-353 FW denotes −
T5378 353-356 CD denotes 445
T5379 357-359 TO denotes to
T5380 360-362 CD denotes +4
T5381 362-363 -RRB- denotes )
T5382 363-364 : denotes :
T5383 365-372 RB denotes forward
T5384 373-374 CD denotes 5
T5385 374-375 SYM denotes ′
T5386 375-376 : denotes -
T5387 376-399 NN denotes TTAATCTAGCCACAGTCATAG-3
T5388 399-400 NN denotes ′
T5389 401-404 CC denotes and
T5390 405-412 NN denotes reverse
T5391 412-413 : denotes :
T5392 414-415 CD denotes 5
T5393 415-416 SYM denotes ′
T5394 416-417 : denotes -
T5395 417-440 NN denotes TCATGGCTCTGAAACGTTCTG-3
T5396 440-441 NN denotes ′
T5397 441-442 . denotes .
T5398 443-446 NNP denotes PCR
T5399 447-450 VBD denotes was
T5400 451-460 VBN denotes performed
T5401 461-466 VBG denotes using
T5402 467-468 DT denotes a
T5403 469-475 NNP denotes Hybaid
T5404 476-484 NNP denotes Omnigene
T5405 485-492 JJ denotes thermal
T5406 493-499 NN denotes cycler
T5407 500-501 -LRB- denotes (
T5408 501-507 NNP denotes Hybaid
T5409 507-508 , denotes ,
T5410 509-516 NNP denotes Ashford
T5411 516-517 , denotes ,
T5412 518-520 NNP denotes UK
T5413 520-521 -RRB- denotes )
T5414 522-526 IN denotes with
T5415 527-534 NN denotes cycling
T5416 535-545 NNS denotes parameters
T5417 546-548 IN denotes of
T5418 549-551 CD denotes 72
T5419 551-552 CD denotes °
T5420 552-553 NNP denotes C
T5421 554-557 IN denotes for
T5422 558-560 CD denotes 10
T5423 561-564 NN denotes min
T5424 564-565 , denotes ,
T5425 566-568 CD denotes 35
T5426 569-575 NNS denotes cycles
T5427 576-578 IN denotes at
T5428 579-581 CD denotes 94
T5429 581-582 CD denotes °
T5430 582-583 NNP denotes C
T5431 584-587 IN denotes for
T5432 588-590 CD denotes 45
T5433 591-592 PRP denotes s
T5434 592-593 , denotes ,
T5435 594-596 CD denotes 52
T5436 596-597 NN denotes °
T5437 597-598 NNP denotes C
T5438 599-602 IN denotes for
T5439 603-605 CD denotes 45
T5440 606-607 PRP denotes s
T5441 607-608 , denotes ,
T5442 609-611 CD denotes 72
T5443 611-612 NN denotes °
T5444 612-613 NNP denotes C
T5445 614-617 IN denotes for
T5446 618-620 CD denotes 45
T5447 621-623 NN denotes s.
R4593 T5318 T5321 compound Chromatin,immunoprecipitation
R4594 T5319 T5321 compound Immunoprecipitation,immunoprecipitation
R4595 T5320 T5321 compound Chromatin,immunoprecipitation
R4596 T5321 T5326 nsubjpass immunoprecipitation,performed
R4597 T5322 T5323 punct (,IP
R4598 T5323 T5321 appos IP,immunoprecipitation
R4599 T5324 T5321 punct ),immunoprecipitation
R4600 T5325 T5326 auxpass was,performed
R4601 T5326 T5326 ROOT performed,performed
R4602 T5327 T5329 mark as,described
R4603 T5328 T5329 advmod previously,described
R4604 T5329 T5326 advcl described,performed
R4605 T5330 T5332 nmod [,]
R4606 T5331 T5332 nummod 12,]
R4607 T5332 T5329 dobj ],described
R4608 T5333 T5329 prep in,described
R4609 T5334 T5335 compound HuT-78,T-cells
R4610 T5335 T5333 pobj T-cells,in
R4611 T5336 T5335 prep with,T-cells
R4612 T5337 T5338 nummod 2,µg
R4613 T5338 T5336 pobj µg,with
R4614 T5339 T5338 prep of,µg
R4615 T5340 T5339 pobj anti-GATA-3,of
R4616 T5341 T5344 punct (,Biotechnology
R4617 T5342 T5344 compound Santa,Biotechnology
R4618 T5343 T5344 compound Cruz,Biotechnology
R4619 T5344 T5340 appos Biotechnology,anti-GATA-3
R4620 T5345 T5344 punct ),Biotechnology
R4621 T5346 T5344 punct ",",Biotechnology
R4622 T5347 T5344 cc or,Biotechnology
R4623 T5348 T5350 amod isotypic,G
R4624 T5349 T5350 compound immunoglobulin,G
R4625 T5350 T5344 conj G,Biotechnology
R4626 T5351 T5338 prep as,µg
R4627 T5352 T5354 det a,control
R4628 T5353 T5354 amod non-specific,control
R4629 T5354 T5351 pobj control,as
R4630 T5355 T5358 punct (,Biotechnology
R4631 T5356 T5357 compound Santa,Cruz
R4632 T5357 T5358 compound Cruz,Biotechnology
R4633 T5358 T5354 appos Biotechnology,control
R4634 T5359 T5354 punct ),control
R4635 T5360 T5329 advmod overnight,described
R4636 T5361 T5329 prep at,described
R4637 T5362 T5363 nummod 4,°
R4638 T5363 T5366 nummod °,sequences
R4639 T5364 T5365 compound C.,Promoter
R4640 T5365 T5366 compound Promoter,sequences
R4641 T5366 T5368 nsubjpass sequences,detected
R4642 T5367 T5368 auxpass were,detected
R4643 T5368 T5326 conj detected,performed
R4644 T5369 T5368 prep with,detected
R4645 T5370 T5371 compound PCR,primers
R4646 T5371 T5369 pobj primers,with
R4647 T5372 T5371 prep for,primers
R4648 T5373 T5375 det the,promoter
R4649 T5374 T5375 compound IL-5,promoter
R4650 T5375 T5372 pobj promoter,for
R4651 T5376 T5375 punct (,promoter
R4652 T5377 T5388 advmod −,′
R4653 T5378 T5388 meta 445,′
R4654 T5379 T5378 prep to,445
R4655 T5380 T5379 pobj +4,to
R4656 T5381 T5378 punct ),445
R4657 T5382 T5388 punct :,′
R4658 T5383 T5388 advmod forward,′
R4659 T5384 T5388 nummod 5,′
R4660 T5385 T5387 punct ′,TTAATCTAGCCACAGTCATAG-3
R4661 T5386 T5387 punct -,TTAATCTAGCCACAGTCATAG-3
R4662 T5387 T5388 compound TTAATCTAGCCACAGTCATAG-3,′
R4663 T5388 T5375 appos ′,promoter
R4664 T5389 T5388 cc and,′
R4665 T5390 T5388 conj reverse,′
R4666 T5391 T5388 punct :,′
R4667 T5392 T5395 nummod 5,TCATGGCTCTGAAACGTTCTG-3
R4687 T5412 T5410 appos UK,Ashford
R4688 T5413 T5408 punct ),Hybaid
R4689 T5414 T5408 prep with,Hybaid
R4690 T5415 T5416 compound cycling,parameters
R4691 T5416 T5414 pobj parameters,with
R4692 T5417 T5416 prep of,parameters
R4693 T5418 T5420 nummod 72,C
R4694 T5419 T5420 compound °,C
R4695 T5420 T5417 pobj C,of
R4696 T5421 T5401 prep for,using
R4697 T5422 T5423 nummod 10,min
R4698 T5423 T5421 pobj min,for
R4699 T5424 T5423 punct ",",min
R4700 T5425 T5426 nummod 35,cycles
R4701 T5426 T5423 appos cycles,min
R4702 T5427 T5401 prep at,using
R4703 T5428 T5429 nummod 94,°
R4704 T5429 T5427 pobj °,at
R4705 T5430 T5401 dobj C,using
R4706 T5431 T5401 prep for,using
R4707 T5432 T5433 nummod 45,s
R4708 T5433 T5431 pobj s,for
R4709 T5434 T5433 punct ",",s
R4710 T5435 T5437 nummod 52,C
R4711 T5436 T5437 compound °,C
R4712 T5437 T5433 appos C,s
R4713 T5438 T5401 prep for,using
R4714 T5439 T5438 pobj 45,for
R4715 T5440 T5401 dobj s,using
R4716 T5441 T5440 punct ",",s
R4717 T5442 T5444 nummod 72,C
R4718 T5443 T5444 compound °,C
R4719 T5444 T5440 appos C,s
R4720 T5445 T5401 prep for,using
R4721 T5446 T5447 nummod 45,s.
R4722 T5447 T5445 pobj s.,for
R4668 T5393 T5395 punct ′,TCATGGCTCTGAAACGTTCTG-3
R4669 T5394 T5395 punct -,TCATGGCTCTGAAACGTTCTG-3
R4670 T5395 T5396 compound TCATGGCTCTGAAACGTTCTG-3,′
R4671 T5396 T5388 appos ′,′
R4672 T5397 T5368 punct .,detected
R4673 T5398 T5400 nsubjpass PCR,performed
R4674 T5399 T5400 auxpass was,performed
R4675 T5400 T5400 ROOT performed,performed
R4676 T5401 T5400 advcl using,performed
R4677 T5402 T5406 det a,cycler
R4678 T5403 T5404 nmod Hybaid,Omnigene
R4679 T5404 T5406 nmod Omnigene,cycler
R4680 T5405 T5406 amod thermal,cycler
R4681 T5406 T5401 dobj cycler,using
R4682 T5407 T5408 punct (,Hybaid
R4683 T5408 T5406 appos Hybaid,cycler
R4684 T5409 T5408 punct ",",Hybaid
R4685 T5410 T5408 npadvmod Ashford,Hybaid
R4686 T5411 T5410 punct ",",Ashford

GO-MF

Id Subject Object Predicate Lexical cue
T5452 191-205 http://purl.obolibrary.org/obo/GO_0003823 denotes immunoglobulin
T5453 337-341 http://purl.obolibrary.org/obo/GO_0005137 denotes IL-5

GO-CC

Id Subject Object Predicate Lexical cue
T5454 0-9 http://purl.obolibrary.org/obo/GO_0000785 denotes Chromatin
T5455 30-39 http://purl.obolibrary.org/obo/GO_0000785 denotes Chromatin
T5456 120-125 http://purl.obolibrary.org/obo/GO_0005623 denotes cells

sentences

Id Subject Object Predicate Lexical cue
T5202 0-29 Sentence denotes Chromatin Immunoprecipitation
T5203 30-413 Sentence denotes Chromatin immunoprecipitation (IP) was performed as previously described [12] in HuT-78 T-cells with 2 µg of anti-GATA-3 (Santa Cruz Biotechnology), or isotypic immunoglobulin G as a non-specific control (Santa Cruz Biotechnology) overnight at 4°C. Promoter sequences were detected with PCR primers for the IL-5 promoter (−445 to +4): forward 5′-TTAATCTAGCCACAGTCATAG-3′ and reverse:
T5204 414-442 Sentence denotes 5′-TCATGGCTCTGAAACGTTCTG-3′.
T5205 443-623 Sentence denotes PCR was performed using a Hybaid Omnigene thermal cycler (Hybaid, Ashford, UK) with cycling parameters of 72°C for 10 min, 35 cycles at 94°C for 45 s, 52°C for 45 s, 72°C for 45 s.
T109 0-29 Sentence denotes Chromatin Immunoprecipitation
T110 30-278 Sentence denotes Chromatin immunoprecipitation (IP) was performed as previously described [12] in HuT-78 T-cells with 2 µg of anti-GATA-3 (Santa Cruz Biotechnology), or isotypic immunoglobulin G as a non-specific control (Santa Cruz Biotechnology) overnight at 4°C.
T111 279-413 Sentence denotes Promoter sequences were detected with PCR primers for the IL-5 promoter (−445 to +4): forward 5′-TTAATCTAGCCACAGTCATAG-3′ and reverse:
T112 414-442 Sentence denotes 5′-TCATGGCTCTGAAACGTTCTG-3′.
T113 443-623 Sentence denotes PCR was performed using a Hybaid Omnigene thermal cycler (Hybaid, Ashford, UK) with cycling parameters of 72°C for 10 min, 35 cycles at 94°C for 45 s, 52°C for 45 s, 72°C for 45 s.

events-check-again

Id Subject Object Predicate Lexical cue
T5459 337-341 Protein denotes IL-5

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T5460 0-9 Protein denotes Chromatin
T5461 10-29 Localization denotes Immunoprecipitation
T5462 30-39 Protein denotes Chromatin
T5463 191-207 Protein denotes immunoglobulin G
T5464 337-350 Protein denotes IL-5 promoter
R4723 T5460 T5461 themeOf Chromatin,Immunoprecipitation

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T5201 337-341 Protein denotes IL-5

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T5316 144-150 P23771 denotes GATA-3
T5317 337-341 P05113 denotes IL-5

test2

Id Subject Object Predicate Lexical cue
T5200 337-341 Protein denotes IL-5