PMC:2674207 / 15162-15677 JSONTXT

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{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2674207","sourcedb":"PMC","sourceid":"2674207","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2674207","text":"tion, and Western Blot Analysis\nNuclear and cytoplasmic fractions were prepared as previously described [35]. Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor. Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris. 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