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PMC:2674207 / 15162-15677 JSONTXT

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2_test

Id Subject Object Predicate Lexical cue
19436703-11395507-86274489 105-107 11395507 denotes 35
T8362 105-107 11395507 denotes 35

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4857 4-5 -COMMA- denotes ,
T4858 6-9 CC denotes and
T4859 10-17 NNP denotes Western
T4860 18-22 NNP denotes Blot
T4861 23-31 NN denotes Analysis
T4862 32-39 JJ denotes Nuclear
T4863 40-43 CC denotes and
T4864 44-55 JJ denotes cytoplasmic
T4865 56-65 NN denotes fractions
T4866 66-70 VB denotes were
T4867 71-79 VB denotes prepared
T4868 80-82 IN denotes as
T4869 83-93 RB denotes previously
T4870 94-103 VB denotes described
T4871 104-105 -LRB- denotes [
T4872 105-107 CD denotes 35
T4873 107-108 -RRB- denotes ]
T4874 110-115 JJ denotes Whole
T4875 116-120 NN denotes cell
T4876 121-128 NN denotes lysates
T4877 129-133 VB denotes were
T4878 134-142 VB denotes prepared
T4879 143-145 IN denotes in
T4880 146-151 NN denotes NP-40
T4881 152-157 NN denotes lysis
T4882 158-164 NN denotes buffer
T4883 165-166 -LRB- denotes (
T4884 166-169 CD denotes 0.5
T4885 169-170 NN denotes %
T4886 171-178 NN denotes Nonidet
T4887 179-183 NN denotes P-40
T4888 183-184 -COMMA- denotes ,
T4889 185-187 CD denotes 20
T4890 188-190 NN denotes mM
T4891 191-199 NN denotes Tris-HCl
T4892 200-201 -LRB- denotes [
T4893 201-203 NN denotes pH
T4894 204-207 CD denotes 7.5
T4895 207-208 -RRB- denotes ]
T4896 208-209 -COMMA- denotes ,
T4897 210-213 CD denotes 150
T4898 214-216 NN denotes mM
T4899 217-221 NN denotes NaCl
T4900 221-222 -RRB- denotes )
T4901 223-225 IN denotes in
T4902 226-229 DT denotes the
T4903 230-238 NN denotes presence
T4904 239-241 IN denotes of
T4905 242-250 JJ denotes complete
T4906 251-259 NN denotes protease
T4907 260-268 NN denotes cocktail
T4908 269-278 NN denotes inhibitor
T4909 280-287 NN denotes Lysates
T4910 288-292 VB denotes were
T4911 293-304 VB denotes centrifuged
T4912 305-307 IN denotes at
T4913 308-311 NN denotes 4°C
T4914 312-315 IN denotes for
T4915 316-318 CD denotes 10
T4916 319-322 NN denotes min
T4917 323-325 IN denotes at
T4918 326-332 CD denotes 12,000
T4919 333-336 NN denotes rpm
T4920 337-339 IN denotes in
T4921 340-342 DT denotes an
T4922 343-352 NNP denotes Eppendorf
T4923 353-368 NN denotes microcentrifuge
T4924 369-371 TO denotes to
T4925 372-378 VB denotes remove
T4926 379-387 JJ denotes cellular
T4927 388-394 NN denotes debris
T4928 396-403 NN denotes Samples
T4929 404-408 VB denotes were
T4930 409-413 RB denotes then
T4931 414-432 VB denotes immunoprecipitated
T4932 433-437 IN denotes with
T4933 438-444 CC denotes either
T4934 445-447 CD denotes 10
T4935 448-450 NN denotes µl
T4936 451-453 IN denotes of
T4937 454-462 NN denotes antibody
T4938 463-470 IN denotes against
T4939 471-477 NN denotes GATA-3
T4940 478-480 CC denotes or
T4941 481-491 NN denotes importin-α
T4942 492-497 VB denotes using
T4943 498-501 NN denotes A/G
T4944 502-509 VB denotes agarose
R4170 T4858 T4857 arg1Of and,","
R4171 T4860 T4859 arg1Of Blot,Western
R4172 T4861 T4858 arg2Of Analysis,and
R4173 T4861 T4860 arg1Of Analysis,Blot
R4174 T4862 T4863 arg1Of Nuclear,and
R4175 T4864 T4863 arg2Of cytoplasmic,and
R4176 T4865 T4862 arg1Of fractions,Nuclear
R4177 T4865 T4864 arg1Of fractions,cytoplasmic
R4178 T4865 T4866 arg1Of fractions,were
R4179 T4865 T4867 arg2Of fractions,prepared
R4180 T4867 T4866 arg2Of prepared,were
R4181 T4867 T4868 arg1Of prepared,as
R4182 T4870 T4869 arg1Of described,previously
R4183 T4872 T4868 arg2Of 35,as
R4184 T4872 T4870 arg1Of 35,described
R4185 T4872 T4871 arg2Of 35,[
R4186 T4873 T4871 arg3Of ],[
R4187 T4876 T4874 arg1Of lysates,Whole
R4188 T4876 T4875 arg1Of lysates,cell
R4189 T4876 T4877 arg1Of lysates,were
R4190 T4876 T4878 arg2Of lysates,prepared
R4191 T4878 T4877 arg2Of prepared,were
R4192 T4878 T4879 arg1Of prepared,in
R4193 T4882 T4879 arg2Of buffer,in
R4194 T4882 T4880 arg1Of buffer,NP-40
R4195 T4882 T4881 arg1Of buffer,lysis
R4196 T4882 T4883 arg1Of buffer,(
R4197 T4882 T4901 arg1Of buffer,in
R4198 T4884 T4885 arg1Of 0.5,%
R4199 T4887 T4884 arg1Of P-40,0.5
R4200 T4887 T4886 arg1Of P-40,Nonidet
R4201 T4887 T4888 arg1Of P-40,","
R4202 T4888 T4896 arg1Of ",",","
R4203 T4889 T4890 arg1Of 20,mM
R4204 T4891 T4888 arg2Of Tris-HCl,","
R4205 T4891 T4889 arg1Of Tris-HCl,20
R4206 T4891 T4892 arg1Of Tris-HCl,[
R4207 T4893 T4892 arg2Of pH,[
R4208 T4893 T4894 arg1Of pH,7.5
R4209 T4895 T4892 arg3Of ],[
R4210 T4896 T4883 arg2Of ",",(
R4211 T4897 T4898 arg1Of 150,mM
R4212 T4899 T4896 arg2Of NaCl,","
R4213 T4899 T4897 arg1Of NaCl,150
R4214 T4900 T4883 arg3Of ),(
R4215 T4903 T4901 arg2Of presence,in
R4216 T4903 T4902 arg1Of presence,the
R4217 T4903 T4904 arg1Of presence,of
R4218 T4908 T4904 arg2Of inhibitor,of
R4219 T4908 T4905 arg1Of inhibitor,complete
R4220 T4908 T4906 arg1Of inhibitor,protease
R4221 T4908 T4907 arg1Of inhibitor,cocktail
R4222 T4909 T4910 arg1Of Lysates,were
R4223 T4909 T4911 arg2Of Lysates,centrifuged
R4224 T4909 T4925 arg1Of Lysates,remove
R4225 T4911 T4910 arg2Of centrifuged,were
R4226 T4911 T4912 arg1Of centrifuged,at
R4227 T4911 T4914 arg1Of centrifuged,for
R4228 T4911 T4917 arg1Of centrifuged,at
R4229 T4911 T4920 arg1Of centrifuged,in
R4230 T4911 T4924 modOf centrifuged,to
R4231 T4913 T4912 arg2Of 4°C,at
R4232 T4916 T4914 arg2Of min,for
R4233 T4916 T4915 arg1Of min,10
R4234 T4919 T4917 arg2Of rpm,at
R4235 T4919 T4918 arg1Of rpm,"12,000"
R4236 T4923 T4920 arg2Of microcentrifuge,in
R4237 T4923 T4921 arg1Of microcentrifuge,an
R4238 T4923 T4922 arg1Of microcentrifuge,Eppendorf
R4239 T4925 T4924 arg1Of remove,to
R4240 T4927 T4925 arg2Of debris,remove
R4241 T4927 T4926 arg1Of debris,cellular
R4242 T4928 T4929 arg1Of Samples,were
R4243 T4928 T4931 arg2Of Samples,immunoprecipitated
R4244 T4931 T4929 arg2Of immunoprecipitated,were
R4245 T4931 T4930 arg1Of immunoprecipitated,then
R4246 T4931 T4932 arg1Of immunoprecipitated,with
R4247 T4935 T4932 arg2Of µl,with
R4248 T4935 T4933 arg1Of µl,either
R4249 T4935 T4934 arg1Of µl,10
R4250 T4935 T4936 arg1Of µl,of
R4251 T4935 T4942 arg1Of µl,using
R4254 T4937 T4936 arg2Of antibody,of
R4255 T4937 T4938 arg1Of antibody,against
R4256 T4939 T4940 arg1Of GATA-3,or
R4257 T4940 T4938 arg2Of or,against
R4258 T4941 T4940 arg2Of importin-α,or

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T5017 4-5 , denotes ,
T5018 6-9 CC denotes and
T5019 10-17 JJ denotes Western
T5020 18-22 NNP denotes Blot
T5021 23-31 NNP denotes Analysis
T5022 32-39 NNP denotes Nuclear
T5023 40-43 CC denotes and
T5024 44-55 JJ denotes cytoplasmic
T5025 56-65 NNS denotes fractions
T5026 66-70 VBD denotes were
T5027 71-79 VBN denotes prepared
T5028 80-82 IN denotes as
T5029 83-93 RB denotes previously
T5030 94-103 VBN denotes described
T5031 104-105 NNP denotes [
T5032 105-107 CD denotes 35
T5033 107-108 NNP denotes ]
T5034 108-109 . denotes .
T5035 110-115 JJ denotes Whole
T5036 116-120 NN denotes cell
T5037 121-128 NNS denotes lysates
T5038 129-133 VBD denotes were
T5039 134-142 VBN denotes prepared
T5040 143-145 IN denotes in
T5041 146-151 NN denotes NP-40
T5042 152-157 NN denotes lysis
T5043 158-164 NN denotes buffer
T5044 165-166 -LRB- denotes (
T5045 166-169 CD denotes 0.5
T5046 169-170 NN denotes %
T5047 171-178 NNP denotes Nonidet
T5048 179-183 NNP denotes P-40
T5049 183-184 , denotes ,
T5050 185-187 CD denotes 20
T5051 188-190 NNP denotes mM
T5052 191-199 NNP denotes Tris-HCl
T5053 200-201 NNP denotes [
T5054 201-203 NNP denotes pH
T5055 204-207 CD denotes 7.5
T5056 207-208 NNP denotes ]
T5057 208-209 , denotes ,
T5058 210-213 CD denotes 150
T5059 214-216 NNP denotes mM
T5060 217-221 NNP denotes NaCl
T5061 221-222 -RRB- denotes )
T5062 223-225 IN denotes in
T5063 226-229 DT denotes the
T5064 230-238 NN denotes presence
T5065 239-241 IN denotes of
T5066 242-250 JJ denotes complete
T5067 251-259 NN denotes protease
T5068 260-268 NN denotes cocktail
T5069 269-278 NN denotes inhibitor
T5070 278-279 . denotes .
T5071 280-287 NNS denotes Lysates
T5072 288-292 VBD denotes were
T5073 293-304 VBN denotes centrifuged
T5074 305-307 IN denotes at
T5075 308-309 CD denotes 4
T5076 309-310 CD denotes °
T5077 310-311 NNP denotes C
T5078 312-315 IN denotes for
T5079 316-318 CD denotes 10
T5080 319-322 NN denotes min
T5081 323-325 IN denotes at
T5082 326-332 CD denotes 12,000
T5083 333-336 NN denotes rpm
T5084 337-339 IN denotes in
T5085 340-342 DT denotes an
T5086 343-352 NNP denotes Eppendorf
T5087 353-368 NN denotes microcentrifuge
T5088 369-371 TO denotes to
T5089 372-378 VB denotes remove
T5090 379-387 JJ denotes cellular
T5091 388-394 NN denotes debris
T5092 394-395 . denotes .
T5093 396-403 NNS denotes Samples
T5094 404-408 VBD denotes were
T5095 409-413 RB denotes then
T5096 414-432 VBN denotes immunoprecipitated
T5097 433-437 IN denotes with
T5098 438-444 DT denotes either
T5099 445-447 CD denotes 10
T5100 448-450 NN denotes µl
T5101 451-453 IN denotes of
T5102 454-462 NN denotes antibody
T5103 463-470 IN denotes against
T5104 471-477 NNP denotes GATA-3
T5105 478-480 CC denotes or
T5106 481-491 JJ denotes importin-α
T5107 492-497 VBG denotes using
T5108 498-501 NNP denotes A/G
T5109 502-509 JJ denotes agarose
R4328 T5019 T5022 amod Western,Nuclear
R4329 T5020 T5022 compound Blot,Nuclear
R4330 T5021 T5022 compound Analysis,Nuclear
R4332 T5023 T5022 cc and,Nuclear
R4333 T5024 T5022 conj cytoplasmic,Nuclear
R4335 T5026 T5027 auxpass were,prepared
R4336 T5027 T5027 ROOT prepared,prepared
R4337 T5028 T5030 mark as,described
R4338 T5029 T5030 advmod previously,described
R4339 T5030 T5027 advcl described,prepared
R4340 T5031 T5033 nmod [,]
R4341 T5032 T5033 nummod 35,]
R4342 T5033 T5030 dobj ],described
R4343 T5034 T5027 punct .,prepared
R4344 T5035 T5037 amod Whole,lysates
R4345 T5036 T5037 compound cell,lysates
R4346 T5037 T5039 nsubjpass lysates,prepared
R4347 T5038 T5039 auxpass were,prepared
R4348 T5039 T5039 ROOT prepared,prepared
R4349 T5040 T5039 prep in,prepared
R4350 T5041 T5043 compound NP-40,buffer
R4351 T5042 T5043 compound lysis,buffer
R4352 T5043 T5040 pobj buffer,in
R4353 T5044 T5048 punct (,P-40
R4354 T5045 T5046 nummod 0.5,%
R4355 T5046 T5048 compound %,P-40
R4356 T5047 T5048 compound Nonidet,P-40
R4357 T5048 T5043 appos P-40,buffer
R4358 T5049 T5048 punct ",",P-40
R4359 T5050 T5054 nummod 20,pH
R4360 T5051 T5054 compound mM,pH
R4361 T5052 T5054 compound Tris-HCl,pH
R4415 T5106 T5104 conj importin-α,GATA-3
R4362 T5053 T5054 compound [,pH
R4363 T5054 T5048 appos pH,P-40
R4364 T5055 T5056 nummod 7.5,]
R4365 T5056 T5056 ROOT ],]
R4366 T5057 T5056 punct ",",]
R4367 T5058 T5060 nummod 150,NaCl
R4368 T5059 T5060 compound mM,NaCl
R4369 T5060 T5056 appos NaCl,]
R4370 T5061 T5056 punct ),]
R4371 T5062 T5056 prep in,]
R4372 T5063 T5064 det the,presence
R4373 T5064 T5062 pobj presence,in
R4374 T5065 T5064 prep of,presence
R4375 T5066 T5069 amod complete,inhibitor
R4376 T5067 T5069 compound protease,inhibitor
R4377 T5068 T5069 compound cocktail,inhibitor
R4378 T5069 T5065 pobj inhibitor,of
R4379 T5070 T5039 punct .,prepared
R4380 T5071 T5073 nsubjpass Lysates,centrifuged
R4381 T5072 T5073 auxpass were,centrifuged
R4382 T5073 T5073 ROOT centrifuged,centrifuged
R4383 T5074 T5073 prep at,centrifuged
R4384 T5075 T5076 nummod 4,°
R4385 T5076 T5074 pobj °,at
R4386 T5077 T5073 conj C,centrifuged
R4387 T5078 T5077 prep for,C
R4388 T5079 T5080 nummod 10,min
R4389 T5080 T5078 pobj min,for
R4390 T5081 T5080 prep at,min
R4391 T5082 T5083 nummod "12,000",rpm
R4392 T5083 T5081 pobj rpm,at
R4393 T5084 T5073 prep in,centrifuged
R4394 T5085 T5087 det an,microcentrifuge
R4395 T5086 T5087 compound Eppendorf,microcentrifuge
R4396 T5087 T5084 pobj microcentrifuge,in
R4397 T5088 T5089 aux to,remove
R4398 T5089 T5073 advcl remove,centrifuged
R4399 T5090 T5091 amod cellular,debris
R4400 T5091 T5089 dobj debris,remove
R4401 T5092 T5073 punct .,centrifuged
R4402 T5093 T5096 nsubjpass Samples,immunoprecipitated
R4403 T5094 T5096 auxpass were,immunoprecipitated
R4404 T5095 T5096 advmod then,immunoprecipitated
R4405 T5096 T5096 ROOT immunoprecipitated,immunoprecipitated
R4406 T5097 T5096 prep with,immunoprecipitated
R4407 T5098 T5100 preconj either,µl
R4408 T5099 T5100 nummod 10,µl
R4409 T5100 T5107 nsubj µl,using
R4410 T5101 T5100 prep of,µl
R4411 T5102 T5101 pobj antibody,of
R4412 T5103 T5102 prep against,antibody
R4413 T5104 T5103 pobj GATA-3,against
R4414 T5105 T5104 cc or,GATA-3
R4416 T5107 T5097 pcomp using,with

GO-BP

Id Subject Object Predicate Lexical cue
T5179 152-157 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T5180 379-387 http://purl.obolibrary.org/obo/GO_0007349 denotes cellular

GO-MF

Id Subject Object Predicate Lexical cue
T5181 454-462 http://purl.obolibrary.org/obo/GO_0003823 denotes antibody

GO-CC

Id Subject Object Predicate Lexical cue
T5183 116-120 http://purl.obolibrary.org/obo/GO_0005623 denotes cell
T5184 454-462 http://purl.obolibrary.org/obo/GO_0019815 denotes antibody
T5185 454-462 http://purl.obolibrary.org/obo/GO_0042571 denotes antibody

sentences

Id Subject Object Predicate Lexical cue
T4847 32-109 Sentence denotes Nuclear and cytoplasmic fractions were prepared as previously described [35].
T4848 110-279 Sentence denotes Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor.
T4849 280-395 Sentence denotes Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris.
T103 32-109 Sentence denotes Nuclear and cytoplasmic fractions were prepared as previously described [35].
T104 110-279 Sentence denotes Whole cell lysates were prepared in NP-40 lysis buffer (0.5% Nonidet P-40, 20 mM Tris-HCl [pH 7.5], 150 mM NaCl) in the presence of complete protease cocktail inhibitor.
T105 280-395 Sentence denotes Lysates were centrifuged at 4°C for 10 min at 12,000 rpm in an Eppendorf microcentrifuge to remove cellular debris.

events-check-again

Id Subject Object Predicate Lexical cue
T5188 471-477 Protein denotes GATA-3

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T5189 242-259 Protein denotes complete protease
T5190 269-278 Negative_regulation denotes inhibitor
T5191 471-477 Protein denotes GATA-3
T5192 481-491 Protein denotes importin-α
R4481 T5189 T5190 themeOf complete protease,inhibitor

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T4845 471-477 Protein denotes GATA-3

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T5004 201-203 P0A7Z4 denotes pH
T5005 471-477 P23771 denotes GATA-3

test2

Id Subject Object Predicate Lexical cue
T4844 471-477 Protein denotes GATA-3