PMC:2674207 / 14566-15125 JSON TXT

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pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

GO-BP

{"project":"GO-BP","denotations":[{"id":"T4829","span":{"begin":0,"end":21},"obj":"http://purl.obolibrary.org/obo/GO_0001171"},{"id":"T4830","span":{"begin":8,"end":21},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T4831","span":{"begin":56,"end":61},"obj":"http://purl.obolibrary.org/obo/GO_0019835"},{"id":"T4832","span":{"begin":288,"end":290},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T4833","span":{"begin":348,"end":350},"obj":"http://purl.obolibrary.org/obo/GO_0003964"}],"text":"Reverse Transcription PCR\nTotal RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK). During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences). Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK). For relative quantification, RT-PCR was carried out using cDNA probes. Primers for IL-4 were from Sigma-Genosys (Cambridge, UK). Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T4834","span":{"begin":288,"end":290},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T4835","span":{"begin":348,"end":350},"obj":"http://purl.obolibrary.org/obo/GO_0003964"},{"id":"T4836","span":{"begin":402,"end":406},"obj":"http://purl.obolibrary.org/obo/GO_0005136"}],"text":"Reverse Transcription PCR\nTotal RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK). During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences). Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK). For relative quantification, RT-PCR was carried out using cDNA probes. Primers for IL-4 were from Sigma-Genosys (Cambridge, UK). Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC."}

sentences

{"project":"sentences","denotations":[{"id":"T4615","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T4616","span":{"begin":26,"end":103},"obj":"Sentence"},{"id":"T4617","span":{"begin":104,"end":199},"obj":"Sentence"},{"id":"T4618","span":{"begin":200,"end":318},"obj":"Sentence"},{"id":"T4619","span":{"begin":319,"end":389},"obj":"Sentence"},{"id":"T4620","span":{"begin":390,"end":447},"obj":"Sentence"},{"id":"T4621","span":{"begin":448,"end":559},"obj":"Sentence"},{"id":"T95","span":{"begin":0,"end":25},"obj":"Sentence"},{"id":"T96","span":{"begin":26,"end":103},"obj":"Sentence"},{"id":"T97","span":{"begin":104,"end":199},"obj":"Sentence"},{"id":"T98","span":{"begin":200,"end":318},"obj":"Sentence"},{"id":"T99","span":{"begin":319,"end":389},"obj":"Sentence"},{"id":"T100","span":{"begin":390,"end":447},"obj":"Sentence"},{"id":"T101","span":{"begin":448,"end":559},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Reverse Transcription PCR\nTotal RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK). During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences). Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK). For relative quantification, RT-PCR was carried out using cDNA probes. Primers for IL-4 were from Sigma-Genosys (Cambridge, UK). Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC."}

events-check-again

{"project":"events-check-again","denotations":[{"id":"T4842","span":{"begin":402,"end":406},"obj":"Protein"}],"text":"Reverse Transcription PCR\nTotal RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK). During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences). Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK). For relative quantification, RT-PCR was carried out using cDNA probes. Primers for IL-4 were from Sigma-Genosys (Cambridge, UK). Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC."}

bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T4839","span":{"begin":159,"end":164},"obj":"Protein"},{"id":"T4840","span":{"begin":402,"end":406},"obj":"Protein"},{"id":"T4841","span":{"begin":461,"end":466},"obj":"Protein"}],"text":"Reverse Transcription PCR\nTotal RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK). During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences). Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK). For relative quantification, RT-PCR was carried out using cDNA probes. Primers for IL-4 were from Sigma-Genosys (Cambridge, UK). Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC."}

bionlp-st-ge-2016-reference

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T4614","span":{"begin":402,"end":406},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Reverse Transcription PCR\nTotal RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK). During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences). Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK). For relative quantification, RT-PCR was carried out using cDNA probes. Primers for IL-4 were from Sigma-Genosys (Cambridge, UK). Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T4715","span":{"begin":323,"end":331},"obj":"Q04864"},{"id":"T4716","span":{"begin":402,"end":406},"obj":"P05112"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Reverse Transcription PCR\nTotal RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK). During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences). Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK). For relative quantification, RT-PCR was carried out using cDNA probes. Primers for IL-4 were from Sigma-Genosys (Cambridge, UK). Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC."}

test2

{"project":"test2","denotations":[{"id":"T4613","span":{"begin":402,"end":406},"obj":"Protein"}],"text":"Reverse Transcription PCR\nTotal RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK). During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences). Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK). For relative quantification, RT-PCR was carried out using cDNA probes. Primers for IL-4 were from Sigma-Genosys (Cambridge, UK). Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC."}

PMC:2674207 / 14566-15125 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:2674207 / 14566-15125 JSON TXT