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PMC:2674207 / 14566-15125 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T4622 0-7 JJ denotes Reverse
T4623 8-21 NN denotes Transcription
T4624 22-25 NN denotes PCR
T4625 26-31 JJ denotes Total
T4626 32-35 NN denotes RNA
T4627 36-39 VB denotes was
T4628 40-49 VB denotes extracted
T4629 50-55 VB denotes using
T4630 56-61 NN denotes lysis
T4631 62-68 NN denotes buffer
T4632 69-70 -LRB- denotes (
T4633 70-76 NN denotes RNeasy
T4634 77-80 NN denotes kit
T4635 80-81 -COLON- denotes ;
T4636 82-88 NNP denotes Qiagen
T4637 88-89 -COMMA- denotes ,
T4638 90-97 NNP denotes Crawley
T4639 97-98 -COMMA- denotes ,
T4640 99-101 NNP denotes UK
T4641 101-102 -RRB- denotes )
T4642 104-110 IN denotes During
T4643 111-114 NN denotes RNA
T4644 115-127 NN denotes purification
T4645 127-128 -COMMA- denotes ,
T4646 129-136 JJ denotes genomic
T4647 137-140 NN denotes DNA
T4648 141-144 VB denotes was
T4649 145-153 VB denotes digested
T4650 154-158 IN denotes with
T4651 159-169 JJ denotes RNase-free
T4652 170-175 NN denotes DNase
T4653 176-177 -LRB- denotes (
T4654 177-185 NNP denotes Amersham
T4655 186-197 NNP denotes Biosciences
T4656 197-198 -RRB- denotes )
T4657 200-204 RB denotes Next
T4658 204-205 -COMMA- denotes ,
T4659 206-209 CD denotes 0.5
T4660 210-212 NN denotes µg
T4661 213-215 IN denotes of
T4662 216-221 JJ denotes total
T4663 222-225 NN denotes RNA
T4664 226-229 VB denotes was
T4665 230-238 VB denotes reversed
T4666 239-250 VB denotes transcribed
T4667 251-256 VB denotes using
T4668 257-260 DT denotes the
T4669 261-266 JJ denotes avian
T4670 267-281 NN denotes myeloblastosis
T4671 282-287 NN denotes virus
T4672 288-290 NN denotes RT
T4673 291-292 -LRB- denotes (
T4674 292-299 NNP denotes Promega
T4675 299-300 -COMMA- denotes ,
T4676 301-312 NNP denotes Southampton
T4677 312-313 -COMMA- denotes ,
T4678 314-316 NNP denotes UK
T4679 316-317 -RRB- denotes )
T4680 319-322 IN denotes For
T4681 323-331 JJ denotes relative
T4682 332-346 NN denotes quantification
T4683 346-347 -COMMA- denotes ,
T4684 348-354 NN denotes RT-PCR
T4685 355-358 VB denotes was
T4686 359-366 VB denotes carried
T4687 367-370 RP denotes out
T4688 371-376 VB denotes using
T4689 377-381 NN denotes cDNA
T4690 382-388 NN denotes probes
T4691 390-397 NN denotes Primers
T4692 398-401 IN denotes for
T4693 402-406 NN denotes IL-4
T4694 407-411 VB denotes were
T4695 412-416 IN denotes from
T4696 417-430 NN denotes Sigma-Genosys
T4697 431-432 -LRB- denotes (
T4698 432-441 NNP denotes Cambridge
T4699 441-442 -COMMA- denotes ,
T4700 443-445 NNP denotes UK
T4701 445-446 -RRB- denotes )
T4702 448-457 NN denotes Sequences
T4703 458-460 IN denotes of
T4704 461-466 NN denotes GADPH
T4705 467-471 VB denotes used
T4706 472-475 VB denotes are
T4707 476-478 IN denotes as
T4708 479-486 VB denotes follows
T4709 486-487 -COLON- denotes :
T4710 488-495 RB denotes forward
T4711 496-522 NN denotes 5′-CCACCCATGGCAAATTCCATGGC
T4712 522-523 -COMMA- denotes ,
T4713 524-531 JJ denotes reverse
T4714 532-558 NN denotes 3′-TCTAGACGGCAGGTCAGGTCCAC
R3969 T4624 T4622 arg1Of PCR,Reverse
R3970 T4624 T4623 arg1Of PCR,Transcription
R3971 T4626 T4625 arg1Of RNA,Total
R3972 T4626 T4627 arg1Of RNA,was
R3973 T4626 T4628 arg2Of RNA,extracted
R3975 T4628 T4627 arg2Of extracted,was
R3979 T4634 T4632 arg2Of kit,(
R3980 T4634 T4633 arg1Of kit,RNeasy
R3981 T4634 T4635 arg1Of kit,;
R3982 T4636 T4635 arg2Of Qiagen,;
R3983 T4636 T4637 arg1Of Qiagen,","
R3984 T4638 T4637 arg2Of Crawley,","
R3985 T4638 T4639 arg1Of Crawley,","
R3986 T4640 T4639 arg2Of UK,","
R3987 T4641 T4632 arg3Of ),(
R3988 T4644 T4642 arg2Of purification,During
R3989 T4644 T4643 arg1Of purification,RNA
R3990 T4647 T4646 arg1Of DNA,genomic
R3991 T4647 T4648 arg1Of DNA,was
R3992 T4647 T4649 arg2Of DNA,digested
R3993 T4649 T4642 arg1Of digested,During
R3994 T4649 T4645 arg1Of digested,","
R3995 T4649 T4648 arg2Of digested,was
R3996 T4649 T4650 arg1Of digested,with
R3997 T4652 T4650 arg2Of DNase,with
R3998 T4652 T4651 arg1Of DNase,RNase-free
R3999 T4652 T4653 arg1Of DNase,(
R4000 T4655 T4653 arg2Of Biosciences,(
R4001 T4655 T4654 arg1Of Biosciences,Amersham
R4002 T4656 T4653 arg3Of ),(
R4003 T4660 T4659 arg1Of µg,0.5
R4004 T4660 T4661 arg1Of µg,of
R4005 T4660 T4664 arg1Of µg,was
R4006 T4660 T4665 arg2Of µg,reversed
R4007 T4663 T4661 arg2Of RNA,of
R4008 T4663 T4662 arg1Of RNA,total
R4009 T4665 T4657 arg1Of reversed,Next
R4010 T4665 T4658 arg1Of reversed,","
R4011 T4665 T4664 arg2Of reversed,was
R4012 T4665 T4666 modOf reversed,transcribed
R4013 T4665 T4667 modOf reversed,using
R4014 T4665 T4673 arg1Of reversed,(
R4015 T4672 T4667 arg2Of RT,using
R4016 T4672 T4668 arg1Of RT,the
R4017 T4672 T4669 arg1Of RT,avian
R4018 T4672 T4670 arg1Of RT,myeloblastosis
R4019 T4672 T4671 arg1Of RT,virus
R4020 T4674 T4675 arg1Of Promega,","
R4021 T4675 T4673 arg2Of ",",(
R4022 T4675 T4677 arg1Of ",",","
R4023 T4676 T4675 arg2Of Southampton,","
R4024 T4678 T4677 arg2Of UK,","
R4025 T4679 T4673 arg3Of ),(
R4026 T4682 T4680 arg2Of quantification,For
R4027 T4682 T4681 arg1Of quantification,relative
R4028 T4684 T4685 arg1Of RT-PCR,was
R4029 T4684 T4686 arg2Of RT-PCR,carried
R4030 T4686 T4680 arg1Of carried,For
R4031 T4686 T4683 arg1Of carried,","
R4032 T4686 T4685 arg2Of carried,was
R4033 T4686 T4687 arg1Of carried,out
R4034 T4686 T4688 modOf carried,using
R4035 T4690 T4688 arg2Of probes,using
R4036 T4690 T4689 arg1Of probes,cDNA
R4037 T4691 T4692 arg1Of Primers,for
R4038 T4691 T4694 arg1Of Primers,were
R4039 T4691 T4695 arg1Of Primers,from
R4040 T4693 T4692 arg2Of IL-4,for
R4041 T4694 T4697 arg1Of were,(
R4042 T4695 T4694 arg2Of from,were
R4043 T4696 T4695 arg2Of Sigma-Genosys,from
R4044 T4698 T4697 arg2Of Cambridge,(
R4045 T4698 T4699 arg1Of Cambridge,","
R4046 T4698 T4700 arg1Of Cambridge,UK
R4047 T4701 T4697 arg3Of ),(
R4048 T4702 T4703 arg1Of Sequences,of
R4049 T4702 T4706 arg1Of Sequences,are
R4050 T4702 T4708 arg1Of Sequences,follows
R4051 T4704 T4703 arg2Of GADPH,of
R4052 T4704 T4705 arg2Of GADPH,used
R4053 T4706 T4707 arg1Of are,as
R4054 T4708 T4707 arg2Of follows,as
R4055 T4708 T4709 arg1Of follows,:
R4056 T4711 T4708 arg2Of 5′-CCACCCATGGCAAATTCCATGGC,follows
R4057 T4711 T4710 arg1Of 5′-CCACCCATGGCAAATTCCATGGC,forward
R4058 T4711 T4712 arg1Of 5′-CCACCCATGGCAAATTCCATGGC,","
R4059 T4714 T4712 arg2Of 3′-TCTAGACGGCAGGTCAGGTCCAC,","
R4060 T4714 T4713 arg1Of 3′-TCTAGACGGCAGGTCAGGTCCAC,reverse
R3974 T4628 T4629 modOf extracted,using
R3976 T4631 T4629 arg2Of buffer,using
R3977 T4631 T4630 arg1Of buffer,lysis
R3978 T4631 T4632 arg1Of buffer,(

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T4717 0-7 VB denotes Reverse
T4718 8-21 NNP denotes Transcription
T4719 22-25 NNP denotes PCR
T4720 26-31 NNP denotes Total
T4721 32-35 NNP denotes RNA
T4722 36-39 VBD denotes was
T4723 40-49 VBN denotes extracted
T4724 50-55 VBG denotes using
T4725 56-61 NN denotes lysis
T4726 62-68 NN denotes buffer
T4727 69-70 -LRB- denotes (
T4728 70-76 NNP denotes RNeasy
T4729 77-80 NN denotes kit
T4730 80-81 : denotes ;
T4731 82-88 NNP denotes Qiagen
T4732 88-89 , denotes ,
T4733 90-97 NNP denotes Crawley
T4734 97-98 , denotes ,
T4735 99-101 NNP denotes UK
T4736 101-102 -RRB- denotes )
T4737 102-103 . denotes .
T4738 104-110 IN denotes During
T4739 111-114 NNP denotes RNA
T4740 115-127 NN denotes purification
T4741 127-128 , denotes ,
T4742 129-136 JJ denotes genomic
T4743 137-140 NN denotes DNA
T4744 141-144 VBD denotes was
T4745 145-153 VBN denotes digested
T4746 154-158 IN denotes with
T4747 159-169 JJ denotes RNase-free
T4748 170-175 NNP denotes DNase
T4749 176-177 -LRB- denotes (
T4750 177-185 NNP denotes Amersham
T4751 186-197 NNPS denotes Biosciences
T4752 197-198 -RRB- denotes )
T4753 198-199 . denotes .
T4754 200-204 JJ denotes Next
T4755 204-205 , denotes ,
T4756 206-209 CD denotes 0.5
T4757 210-212 NN denotes µg
T4758 213-215 IN denotes of
T4759 216-221 JJ denotes total
T4760 222-225 NNP denotes RNA
T4761 226-229 VBD denotes was
T4762 230-238 JJ denotes reversed
T4763 239-250 VBN denotes transcribed
T4764 251-256 VBG denotes using
T4765 257-260 DT denotes the
T4766 261-266 JJ denotes avian
T4767 267-281 NN denotes myeloblastosis
T4768 282-287 NN denotes virus
T4769 288-290 NNP denotes RT
T4770 291-292 -LRB- denotes (
T4771 292-299 NNP denotes Promega
T4772 299-300 , denotes ,
T4773 301-312 NNP denotes Southampton
T4774 312-313 , denotes ,
T4775 314-316 NNP denotes UK
T4776 316-317 -RRB- denotes )
T4777 317-318 . denotes .
T4778 319-322 IN denotes For
T4779 323-331 JJ denotes relative
T4780 332-346 NN denotes quantification
T4781 346-347 , denotes ,
T4782 348-354 NN denotes RT-PCR
T4783 355-358 VBD denotes was
T4784 359-366 VBN denotes carried
T4785 367-370 IN denotes out
T4786 371-376 VBG denotes using
T4787 377-381 NNP denotes cDNA
T4788 382-388 NNS denotes probes
T4789 388-389 . denotes .
T4790 390-397 NNS denotes Primers
T4791 398-401 IN denotes for
T4792 402-406 NN denotes IL-4
T4793 407-411 VBD denotes were
T4794 412-416 IN denotes from
T4795 417-430 NNP denotes Sigma-Genosys
T4796 431-432 -LRB- denotes (
T4797 432-441 NNP denotes Cambridge
T4798 441-442 , denotes ,
T4799 443-445 NNP denotes UK
T4800 445-446 -RRB- denotes )
T4801 446-447 . denotes .
T4802 448-457 NNS denotes Sequences
T4803 458-460 IN denotes of
T4804 461-466 NNP denotes GADPH
T4805 467-471 VBD denotes used
T4806 472-475 VBP denotes are
T4807 476-478 IN denotes as
T4808 479-486 VBZ denotes follows
T4809 486-487 : denotes :
T4810 488-495 RB denotes forward
T4811 496-497 CD denotes 5
T4812 497-498 SYM denotes ′
T4813 498-499 : denotes -
T4814 499-522 NNP denotes CCACCCATGGCAAATTCCATGGC
T4815 522-523 , denotes ,
T4816 524-531 VB denotes reverse
T4817 532-533 CD denotes 3
T4818 533-534 SYM denotes ′
T4819 534-535 : denotes -
T4820 535-558 NNP denotes TCTAGACGGCAGGTCAGGTCCAC
T4821 558-559 . denotes .
R4061 T4717 T4721 amod Reverse,RNA
R4062 T4718 T4721 compound Transcription,RNA
R4063 T4719 T4721 compound PCR,RNA
R4064 T4720 T4721 compound Total,RNA
R4065 T4721 T4723 nsubjpass RNA,extracted
R4066 T4722 T4723 auxpass was,extracted
R4067 T4723 T4723 ROOT extracted,extracted
R4068 T4724 T4723 advcl using,extracted
R4069 T4725 T4726 compound lysis,buffer
R4070 T4726 T4724 dobj buffer,using
R4071 T4727 T4726 punct (,buffer
R4072 T4728 T4729 compound RNeasy,kit
R4073 T4729 T4726 appos kit,buffer
R4074 T4730 T4726 punct ;,buffer
R4075 T4731 T4726 conj Qiagen,buffer
R4076 T4732 T4731 punct ",",Qiagen
R4077 T4733 T4731 conj Crawley,Qiagen
R4078 T4734 T4733 punct ",",Crawley
R4079 T4735 T4733 conj UK,Crawley
R4080 T4736 T4731 punct ),Qiagen
R4081 T4737 T4723 punct .,extracted
R4082 T4738 T4745 prep During,digested
R4083 T4739 T4740 compound RNA,purification
R4084 T4740 T4738 pobj purification,During
R4085 T4741 T4745 punct ",",digested
R4086 T4742 T4743 amod genomic,DNA
R4087 T4743 T4745 nsubjpass DNA,digested
R4088 T4744 T4745 auxpass was,digested
R4089 T4745 T4745 ROOT digested,digested
R4090 T4746 T4745 prep with,digested
R4091 T4747 T4748 compound RNase-free,DNase
R4092 T4748 T4746 pobj DNase,with
R4093 T4749 T4751 punct (,Biosciences
R4094 T4750 T4751 compound Amersham,Biosciences
R4095 T4751 T4748 appos Biosciences,DNase
R4096 T4752 T4751 punct ),Biosciences
R4097 T4753 T4745 punct .,digested
R4098 T4754 T4757 advmod Next,µg
R4099 T4755 T4757 punct ",",µg
R4100 T4756 T4757 nummod 0.5,µg
R4101 T4757 T4763 nsubjpass µg,transcribed
R4102 T4758 T4757 prep of,µg
R4103 T4759 T4760 amod total,RNA
R4104 T4760 T4758 pobj RNA,of
R4105 T4761 T4763 auxpass was,transcribed
R4106 T4762 T4763 advmod reversed,transcribed
R4107 T4763 T4763 ROOT transcribed,transcribed
R4108 T4764 T4763 advcl using,transcribed
R4109 T4765 T4768 det the,virus
R4110 T4766 T4767 amod avian,myeloblastosis
R4111 T4767 T4768 compound myeloblastosis,virus
R4134 T4790 T4793 nsubj Primers,were
R4135 T4791 T4790 prep for,Primers
R4136 T4792 T4791 pobj IL-4,for
R4137 T4793 T4793 ROOT were,were
R4138 T4794 T4793 prep from,were
R4139 T4795 T4794 pobj Sigma-Genosys,from
R4140 T4796 T4795 punct (,Sigma-Genosys
R4141 T4797 T4795 appos Cambridge,Sigma-Genosys
R4142 T4798 T4797 punct ",",Cambridge
R4143 T4799 T4797 appos UK,Cambridge
R4144 T4800 T4797 punct ),Cambridge
R4145 T4801 T4793 punct .,were
R4146 T4802 T4806 nsubj Sequences,are
R4147 T4803 T4802 prep of,Sequences
R4148 T4804 T4803 pobj GADPH,of
R4149 T4805 T4802 acl used,Sequences
R4150 T4806 T4806 ROOT are,are
R4151 T4807 T4808 mark as,follows
R4152 T4808 T4806 advcl follows,are
R4153 T4809 T4806 punct :,are
R4154 T4810 T4816 advmod forward,reverse
R4155 T4811 T4814 nummod 5,CCACCCATGGCAAATTCCATGGC
R4156 T4812 T4814 punct ′,CCACCCATGGCAAATTCCATGGC
R4157 T4813 T4814 punct -,CCACCCATGGCAAATTCCATGGC
R4158 T4814 T4816 npadvmod CCACCCATGGCAAATTCCATGGC,reverse
R4159 T4815 T4816 punct ",",reverse
R4160 T4816 T4820 dep reverse,TCTAGACGGCAGGTCAGGTCCAC
R4161 T4817 T4816 dobj 3,reverse
R4162 T4818 T4820 punct ′,TCTAGACGGCAGGTCAGGTCCAC
R4163 T4819 T4820 punct -,TCTAGACGGCAGGTCAGGTCCAC
R4164 T4820 T4820 ROOT TCTAGACGGCAGGTCAGGTCCAC,TCTAGACGGCAGGTCAGGTCCAC
R4165 T4821 T4820 punct .,TCTAGACGGCAGGTCAGGTCCAC
R4112 T4768 T4764 dobj virus,using
R4113 T4769 T4771 nmod RT,Promega
R4114 T4770 T4771 punct (,Promega
R4115 T4771 T4768 appos Promega,virus
R4116 T4772 T4771 punct ",",Promega
R4117 T4773 T4771 conj Southampton,Promega
R4118 T4774 T4773 punct ",",Southampton
R4119 T4775 T4773 conj UK,Southampton
R4120 T4776 T4771 punct ),Promega
R4121 T4777 T4763 punct .,transcribed
R4122 T4778 T4784 prep For,carried
R4123 T4779 T4780 amod relative,quantification
R4124 T4780 T4778 pobj quantification,For
R4125 T4781 T4784 punct ",",carried
R4126 T4782 T4784 nsubjpass RT-PCR,carried
R4127 T4783 T4784 auxpass was,carried
R4128 T4784 T4784 ROOT carried,carried
R4129 T4785 T4784 prt out,carried
R4130 T4786 T4784 advcl using,carried
R4131 T4787 T4788 compound cDNA,probes
R4132 T4788 T4786 dobj probes,using
R4133 T4789 T4784 punct .,carried

GO-BP

Id Subject Object Predicate Lexical cue
T4829 0-21 http://purl.obolibrary.org/obo/GO_0001171 denotes Reverse Transcription
T4830 8-21 http://purl.obolibrary.org/obo/GO_0006351 denotes Transcription
T4831 56-61 http://purl.obolibrary.org/obo/GO_0019835 denotes lysis
T4832 288-290 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T4833 348-350 http://purl.obolibrary.org/obo/GO_0003964 denotes RT

GO-MF

Id Subject Object Predicate Lexical cue
T4834 288-290 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T4835 348-350 http://purl.obolibrary.org/obo/GO_0003964 denotes RT
T4836 402-406 http://purl.obolibrary.org/obo/GO_0005136 denotes IL-4

sentences

Id Subject Object Predicate Lexical cue
T4615 0-25 Sentence denotes Reverse Transcription PCR
T4616 26-103 Sentence denotes Total RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK).
T4617 104-199 Sentence denotes During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences).
T4618 200-318 Sentence denotes Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK).
T4619 319-389 Sentence denotes For relative quantification, RT-PCR was carried out using cDNA probes.
T4620 390-447 Sentence denotes Primers for IL-4 were from Sigma-Genosys (Cambridge, UK).
T4621 448-559 Sentence denotes Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC.
T95 0-25 Sentence denotes Reverse Transcription PCR
T96 26-103 Sentence denotes Total RNA was extracted using lysis buffer (RNeasy kit; Qiagen, Crawley, UK).
T97 104-199 Sentence denotes During RNA purification, genomic DNA was digested with RNase-free DNase (Amersham Biosciences).
T98 200-318 Sentence denotes Next, 0.5 µg of total RNA was reversed transcribed using the avian myeloblastosis virus RT (Promega, Southampton, UK).
T99 319-389 Sentence denotes For relative quantification, RT-PCR was carried out using cDNA probes.
T100 390-447 Sentence denotes Primers for IL-4 were from Sigma-Genosys (Cambridge, UK).
T101 448-559 Sentence denotes Sequences of GADPH used are as follows: forward 5′-CCACCCATGGCAAATTCCATGGC, reverse 3′-TCTAGACGGCAGGTCAGGTCCAC.

events-check-again

Id Subject Object Predicate Lexical cue
T4842 402-406 Protein denotes IL-4

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T4839 159-164 Protein denotes RNase
T4840 402-406 Protein denotes IL-4
T4841 461-466 Protein denotes GADPH

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T4614 402-406 Protein denotes IL-4

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T4715 323-331 Q04864 denotes relative
T4716 402-406 P05112 denotes IL-4

test2

Id Subject Object Predicate Lexical cue
T4613 402-406 Protein denotes IL-4