PMC:2664230 / 12528-13416 JSONTXT

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of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}

{"project":"GO-BP","denotations":[{"id":"T5960","span":{"begin":8,"end":11},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5961","span":{"begin":85,"end":88},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5962","span":{"begin":251,"end":254},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5963","span":{"begin":558,"end":561},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5964","span":{"begin":727,"end":730},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5965","span":{"begin":44,"end":60},"obj":"http://purl.obolibrary.org/obo/GO_0032680"},{"id":"T5966","span":{"begin":92,"end":108},"obj":"http://purl.obolibrary.org/obo/GO_0032680"},{"id":"T5967","span":{"begin":184,"end":200},"obj":"http://purl.obolibrary.org/obo/GO_0032680"},{"id":"T5968","span":{"begin":44,"end":60},"obj":"http://purl.obolibrary.org/obo/GO_0032640"},{"id":"T5969","span":{"begin":92,"end":108},"obj":"http://purl.obolibrary.org/obo/GO_0032640"},{"id":"T5970","span":{"begin":184,"end":200},"obj":"http://purl.obolibrary.org/obo/GO_0032640"}],"text":"Role of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}

{"project":"GO-MF","denotations":[{"id":"T5971","span":{"begin":8,"end":11},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5972","span":{"begin":85,"end":88},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5973","span":{"begin":251,"end":254},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5974","span":{"begin":558,"end":561},"obj":"http://purl.obolibrary.org/obo/GO_0004691"},{"id":"T5975","span":{"begin":727,"end":730},"obj":"http://purl.obolibrary.org/obo/GO_0004691"}],"text":"Role of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}

{"project":"GO-CC","denotations":[{"id":"T5978","span":{"begin":251,"end":254},"obj":"http://purl.obolibrary.org/obo/GO_0005952"},{"id":"T5979","span":{"begin":558,"end":561},"obj":"http://purl.obolibrary.org/obo/GO_0005952"},{"id":"T5980","span":{"begin":727,"end":730},"obj":"http://purl.obolibrary.org/obo/GO_0005952"},{"id":"T5981","span":{"begin":39,"end":43},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5982","span":{"begin":124,"end":129},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5983","span":{"begin":301,"end":306},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T5976","span":{"begin":8,"end":11},"obj":"http://purl.obolibrary.org/obo/GO_0005952"},{"id":"T5977","span":{"begin":85,"end":88},"obj":"http://purl.obolibrary.org/obo/GO_0005952"}],"text":"Role of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}

{"project":"sentences","denotations":[{"id":"T5643","span":{"begin":0,"end":60},"obj":"Sentence"},{"id":"T5644","span":{"begin":61,"end":279},"obj":"Sentence"},{"id":"T5645","span":{"begin":280,"end":452},"obj":"Sentence"},{"id":"T5646","span":{"begin":453,"end":771},"obj":"Sentence"},{"id":"T5647","span":{"begin":772,"end":852},"obj":"Sentence"},{"id":"T5648","span":{"begin":853,"end":888},"obj":"Sentence"},{"id":"T93","span":{"begin":0,"end":60},"obj":"Sentence"},{"id":"T94","span":{"begin":61,"end":279},"obj":"Sentence"},{"id":"T95","span":{"begin":280,"end":452},"obj":"Sentence"},{"id":"T96","span":{"begin":453,"end":771},"obj":"Sentence"},{"id":"T97","span":{"begin":772,"end":852},"obj":"Sentence"},{"id":"T98","span":{"begin":853,"end":888},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Role of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}

{"project":"events-check-again","denotations":[{"id":"T6040","span":{"begin":0,"end":4},"obj":"Regulation"},{"id":"T6041","span":{"begin":19,"end":26},"obj":"Positive_regulation"},{"id":"T6042","span":{"begin":44,"end":49},"obj":"Protein"},{"id":"T6043","span":{"begin":50,"end":60},"obj":"Gene_expression"},{"id":"T6044","span":{"begin":77,"end":81},"obj":"Regulation"},{"id":"T6045","span":{"begin":92,"end":97},"obj":"Protein"},{"id":"T6046","span":{"begin":98,"end":108},"obj":"Gene_expression"},{"id":"T6047","span":{"begin":138,"end":149},"obj":"Regulation"},{"id":"T6048","span":{"begin":157,"end":168},"obj":"Negative_regulation"},{"id":"T6049","span":{"begin":176,"end":183},"obj":"Positive_regulation"},{"id":"T6050","span":{"begin":184,"end":189},"obj":"Protein"},{"id":"T6051","span":{"begin":190,"end":200},"obj":"Gene_expression"},{"id":"T6052","span":{"begin":776,"end":781},"obj":"Protein"}],"relations":[{"id":"R5017","pred":"themeOf","subj":"T6041","obj":"T6040"},{"id":"R5018","pred":"themeOf","subj":"T6042","obj":"T6043"},{"id":"R5019","pred":"themeOf","subj":"T6043","obj":"T6041"},{"id":"R5020","pred":"themeOf","subj":"T6045","obj":"T6046"},{"id":"R5021","pred":"themeOf","subj":"T6046","obj":"T6044"},{"id":"R5022","pred":"themeOf","subj":"T6048","obj":"T6047"},{"id":"R5023","pred":"themeOf","subj":"T6049","obj":"T6048"},{"id":"R5024","pred":"themeOf","subj":"T6050","obj":"T6051"},{"id":"R5025","pred":"themeOf","subj":"T6051","obj":"T6049"}],"text":"Role of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T5984","span":{"begin":8,"end":11},"obj":"Protein"},{"id":"T5985","span":{"begin":44,"end":49},"obj":"Protein"},{"id":"T5986","span":{"begin":50,"end":60},"obj":"Gene_expression"},{"id":"T5987","span":{"begin":0,"end":4},"obj":"Regulation"},{"id":"T5988","span":{"begin":15,"end":26},"obj":"Positive_regulation"},{"id":"T5989","span":{"begin":85,"end":88},"obj":"Protein"},{"id":"T5990","span":{"begin":92,"end":97},"obj":"Protein"},{"id":"T5991","span":{"begin":184,"end":189},"obj":"Protein"},{"id":"T5992","span":{"begin":251,"end":254},"obj":"Protein"},{"id":"T5993","span":{"begin":98,"end":108},"obj":"Gene_expression"},{"id":"T5994","span":{"begin":190,"end":200},"obj":"Gene_expression"},{"id":"T5995","span":{"begin":255,"end":264},"obj":"Negative_regulation"},{"id":"T5996","span":{"begin":77,"end":81},"obj":"Regulation"},{"id":"T5997","span":{"begin":157,"end":168},"obj":"Negative_regulation"},{"id":"T5998","span":{"begin":138,"end":149},"obj":"Regulation"},{"id":"T5999","span":{"begin":558,"end":561},"obj":"Protein"},{"id":"T6000","span":{"begin":727,"end":730},"obj":"Protein"},{"id":"T6001","span":{"begin":544,"end":554},"obj":"Negative_regulation"},{"id":"T6002","span":{"begin":731,"end":741},"obj":"Negative_regulation"},{"id":"T6003","span":{"begin":776,"end":781},"obj":"Protein"}],"relations":[{"id":"R4980","pred":"causeOf","subj":"T5984","obj":"T5987"},{"id":"R4981","pred":"themeOf","subj":"T5985","obj":"T5986"},{"id":"R4982","pred":"themeOf","subj":"T5986","obj":"T5987"},{"id":"R4983","pred":"themeOf","subj":"T5986","obj":"T5988"},{"id":"R4984","pred":"causeOf","subj":"T5989","obj":"T5996"},{"id":"R4985","pred":"themeOf","subj":"T5990","obj":"T5993"},{"id":"R4986","pred":"themeOf","subj":"T5991","obj":"T5994"},{"id":"R4987","pred":"themeOf","subj":"T5992","obj":"T5995"},{"id":"R4988","pred":"themeOf","subj":"T5993","obj":"T5996"},{"id":"R4989","pred":"themeOf","subj":"T5994","obj":"T5997"},{"id":"R4990","pred":"themeOf","subj":"T5997","obj":"T5998"},{"id":"R4991","pred":"themeOf","subj":"T5999","obj":"T6001"},{"id":"R4992","pred":"themeOf","subj":"T6000","obj":"T6002"}],"text":"Role of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T5942","span":{"begin":44,"end":47},"obj":"P01375"},{"id":"T5943","span":{"begin":44,"end":49},"obj":"P01375"},{"id":"T5944","span":{"begin":92,"end":95},"obj":"P01375"},{"id":"T5945","span":{"begin":92,"end":97},"obj":"P01375"},{"id":"T5946","span":{"begin":184,"end":187},"obj":"P01375"},{"id":"T5947","span":{"begin":184,"end":189},"obj":"P01375"},{"id":"T5948","span":{"begin":776,"end":779},"obj":"P01375"},{"id":"T5949","span":{"begin":776,"end":781},"obj":"P01375"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Role of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}

{"project":"test2","denotations":[{"id":"T5611","span":{"begin":19,"end":26},"obj":"Positive_regulation"},{"id":"T5612","span":{"begin":44,"end":49},"obj":"Protein"},{"id":"T5613","span":{"begin":50,"end":60},"obj":"Gene_expression"},{"id":"T5614","span":{"begin":77,"end":81},"obj":"Regulation"},{"id":"T5615","span":{"begin":92,"end":97},"obj":"Protein"},{"id":"T5616","span":{"begin":98,"end":108},"obj":"Gene_expression"},{"id":"T5617","span":{"begin":176,"end":183},"obj":"Positive_regulation"},{"id":"T5618","span":{"begin":184,"end":189},"obj":"Protein"},{"id":"T5619","span":{"begin":190,"end":200},"obj":"Gene_expression"},{"id":"T5620","span":{"begin":210,"end":219},"obj":"Positive_regulation"},{"id":"T5621","span":{"begin":776,"end":781},"obj":"Protein"}],"relations":[{"id":"R4660","pred":"themeOf","subj":"T5612","obj":"T5613"},{"id":"R4661","pred":"themeOf","subj":"T5613","obj":"T5611"},{"id":"R4662","pred":"themeOf","subj":"T5615","obj":"T5616"},{"id":"R4663","pred":"themeOf","subj":"T5617","obj":"T5620"},{"id":"R4664","pred":"themeOf","subj":"T5618","obj":"T5619"},{"id":"R4665","pred":"themeOf","subj":"T5619","obj":"T5620"},{"id":"R4666","pred":"themeOf","subj":"T5619","obj":"T5617"}],"text":"Role of PKA on LPS-induced mononuclear cell TNF-α production\nTo evaluate the role of PKA on TNF-α production in mononuclear cells and its involvement in the attenuation of LPS-induced TNF-α production observed following PTX treatment, H89, a specific PKA inhibitor, was utilized. Isolated mononuclear cells were incubated according to the treatment groups described above in the presence and absence of pretreatment with H89 (10 μM) for 1 hour at 37ºC. The dose of H89 was chosen based on previous work which demonstrated specific and complete inhibition of PKA at this concentration.10 Since the activity of this inhibitor is both specific and consistent at this specified dose, we did not include experiments with additional PKA inhibitors with this set of experiments. The TNF-α concentration in the supernatant was measured quantitatively by ELISA. The results are expressed in pg/mL."}