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2_test

{"project":"2_test","denotations":[{"id":"19139168-16273099-59674076","span":{"begin":699,"end":700},"obj":"16273099"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

GO-BP

{"project":"GO-BP","denotations":[{"id":"T16652","span":{"begin":11,"end":23},"obj":"http://purl.obolibrary.org/obo/GO_0009293"},{"id":"T16653","span":{"begin":53,"end":65},"obj":"http://purl.obolibrary.org/obo/GO_0009293"},{"id":"T16654","span":{"begin":249,"end":252},"obj":"http://purl.obolibrary.org/obo/GO_0006283"},{"id":"T16655","span":{"begin":501,"end":503},"obj":"http://purl.obolibrary.org/obo/GO_0003964"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T16913","span":{"begin":501,"end":503},"obj":"http://purl.obolibrary.org/obo/GO_0003964"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T16914","span":{"begin":42,"end":47},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T16915","span":{"begin":158,"end":163},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T16916","span":{"begin":278,"end":283},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T16917","span":{"begin":249,"end":252},"obj":"http://purl.obolibrary.org/obo/GO_0042101"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

sentences

{"project":"sentences","denotations":[{"id":"T16647","span":{"begin":0,"end":48},"obj":"Sentence"},{"id":"T16648","span":{"begin":49,"end":221},"obj":"Sentence"},{"id":"T16649","span":{"begin":222,"end":452},"obj":"Sentence"},{"id":"T16650","span":{"begin":453,"end":551},"obj":"Sentence"},{"id":"T16651","span":{"begin":552,"end":702},"obj":"Sentence"},{"id":"T182","span":{"begin":0,"end":48},"obj":"Sentence"},{"id":"T183","span":{"begin":49,"end":221},"obj":"Sentence"},{"id":"T184","span":{"begin":222,"end":452},"obj":"Sentence"},{"id":"T185","span":{"begin":453,"end":551},"obj":"Sentence"},{"id":"T186","span":{"begin":552,"end":643},"obj":"Sentence"},{"id":"T187","span":{"begin":644,"end":702},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

events-check-again

{"project":"events-check-again","denotations":[{"id":"T16929","span":{"begin":578,"end":588},"obj":"Protein"},{"id":"T16928","span":{"begin":271,"end":274},"obj":"Protein"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T16930","span":{"begin":35,"end":39},"obj":"Protein"},{"id":"T16931","span":{"begin":249,"end":252},"obj":"Protein"},{"id":"T16932","span":{"begin":271,"end":274},"obj":"Protein"},{"id":"T16933","span":{"begin":578,"end":583},"obj":"Protein"},{"id":"T16934","span":{"begin":584,"end":588},"obj":"Protein"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

bionlp-st-ge-2016-reference

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T16645","span":{"begin":271,"end":274},"obj":"Protein"},{"id":"T16646","span":{"begin":578,"end":588},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T16903","span":{"begin":35,"end":38},"obj":"P10966"},{"id":"T16904","span":{"begin":35,"end":38},"obj":"P01732"},{"id":"T16905","span":{"begin":271,"end":274},"obj":"P10966"},{"id":"T16906","span":{"begin":271,"end":274},"obj":"P01732"},{"id":"T16907","span":{"begin":578,"end":583},"obj":"O95936"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

test2

{"project":"test2","denotations":[{"id":"T16643","span":{"begin":271,"end":274},"obj":"Protein"},{"id":"T16644","span":{"begin":578,"end":588},"obj":"Protein"}],"text":"Retroviral transduction of primary CD8+ T cells.\nFor transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g. At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus. The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h. Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8)."}

PMC:2626671 / 30353-31055 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:2626671 / 30353-31055 JSON TXT