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PMC:2626671 / 30353-31055 JSONTXT

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2_test

Id Subject Object Predicate Lexical cue
19139168-16273099-59674076 699-700 16273099 denotes 8

pmc-enju-pas

Id Subject Object Predicate Lexical cue
T16785 0-10 JJ denotes Retroviral
T16786 11-23 NN denotes transduction
T16787 24-26 IN denotes of
T16788 27-34 JJ denotes primary
T16789 35-39 JJ denotes CD8+
T16790 40-41 NN denotes T
T16791 42-47 NN denotes cells
T16792 49-52 IN denotes For
T16793 53-65 NN denotes transduction
T16794 66-77 NN denotes experiments
T16795 77-78 -COMMA- denotes ,
T16796 79-84 JJ denotes viral
T16797 85-97 NN denotes supernatants
T16798 98-102 VB denotes were
T16799 103-112 VB denotes generated
T16800 113-115 IN denotes by
T16801 116-123 NN denotes calcium
T16802 124-133 NN denotes phosphate
T16803 134-146 NN denotes transfection
T16804 147-149 IN denotes of
T16805 150-157 NN denotes Phoenix
T16806 158-163 NN denotes cells
T16807 164-167 CC denotes and
T16808 168-181 NN denotes concentration
T16809 182-184 IN denotes by
T16810 185-194 JJ denotes overnight
T16811 195-209 NN denotes centrifugation
T16812 210-212 IN denotes at
T16813 213-218 CD denotes 6,000
T16814 219-220 NN denotes g
T16815 222-224 IN denotes At
T16816 225-228 CD denotes ∼42
T16817 229-230 NN denotes h
T16818 231-236 IN denotes after
T16819 237-240 DT denotes the
T16820 241-248 JJ denotes initial
T16821 249-252 NN denotes TCR
T16822 253-263 NN denotes activation
T16823 264-266 IN denotes of
T16824 267-270 CD denotes 106
T16825 271-275 JJ denotes CD8+
T16826 276-277 NN denotes T
T16827 278-283 NN denotes cells
T16828 284-287 IN denotes per
T16829 288-292 RB denotes well
T16830 293-295 IN denotes in
T16831 296-303 JJ denotes 12-well
T16832 304-310 NN denotes plates
T16833 310-311 -COMMA- denotes ,
T16834 312-315 DT denotes the
T16835 316-323 NN denotes culture
T16836 324-329 NN denotes media
T16837 330-333 VB denotes was
T16838 334-341 VB denotes removed
T16839 342-345 CC denotes and
T16840 346-354 VB denotes replaced
T16841 355-359 IN denotes with
T16842 360-368 JJ denotes complete
T16843 369-374 NN denotes media
T16844 375-387 VB denotes supplemented
T16845 388-392 IN denotes with
T16846 393-394 CD denotes 8
T16847 395-400 NN denotes μg/ml
T16848 401-410 NN denotes polybrene
T16849 411-421 VB denotes containing
T16850 422-427 JJ denotes fresh
T16851 428-432 CC denotes plus
T16852 433-445 JJ denotes concentrated
T16853 446-451 NN denotes virus
T16854 453-456 DT denotes The
T16855 457-463 NN denotes plates
T16856 464-468 VB denotes were
T16857 469-480 VB denotes centrifuged
T16858 481-483 IN denotes at
T16859 484-487 CD denotes 700
T16860 488-489 NN denotes g
T16861 490-493 IN denotes for
T16862 494-495 CD denotes 1
T16863 496-497 NN denotes h
T16864 498-500 IN denotes at
T16865 501-503 NN denotes RT
T16866 504-510 IN denotes before
T16867 511-520 VB denotes returning
T16868 521-523 TO denotes to
T16869 524-528 NN denotes 37°C
T16870 529-532 IN denotes for
T16871 533-535 DT denotes an
T16872 536-546 JJ denotes additional
T16873 547-548 CD denotes 5
T16874 549-550 NN denotes h
T16875 552-562 JJ denotes Retroviral
T16876 563-573 NN denotes constructs
T16877 574-577 IN denotes for
T16878 578-588 NN denotes Eomes-VP16
T16879 589-592 CC denotes and
T16880 593-596 DT denotes the
T16881 597-600 NN denotes MIG
T16882 601-608 NN denotes control
T16883 609-614 JJ denotes empty
T16884 615-621 NN denotes vector
T16885 622-626 VB denotes were
T16886 627-628 DT denotes a
T16887 629-633 NN denotes gift
T16888 634-638 IN denotes from
T16889 639-643 NNP denotes S.L.
T16890 644-650 NNP denotes Reiner
T16891 651-652 -LRB- denotes (
T16892 652-662 NNP denotes University
T16893 663-665 IN denotes of
T16894 666-678 NNP denotes Pennsylvania
T16895 678-679 -COMMA- denotes ,
T16896 680-692 NNP denotes Philadelphia
T16897 692-693 -COMMA- denotes ,
T16898 694-696 NNP denotes PA
T16899 696-697 -RRB- denotes )
T16900 698-699 -LRB- denotes (
T16901 699-700 CD denotes 8
T16902 700-701 -RRB- denotes )
R12928 T16786 T16785 arg1Of transduction,Retroviral
R12929 T16786 T16787 arg1Of transduction,of
R12930 T16791 T16787 arg2Of cells,of
R12931 T16791 T16788 arg1Of cells,primary
R12932 T16791 T16789 arg1Of cells,CD8+
R12933 T16791 T16790 arg1Of cells,T
R12935 T16794 T16792 arg2Of experiments,For
R12936 T16797 T16796 arg1Of supernatants,viral
R12937 T16797 T16798 arg1Of supernatants,were
R12938 T16797 T16799 arg2Of supernatants,generated
R12940 T16799 T16792 arg1Of generated,For
R12941 T16799 T16798 arg2Of generated,were
R12942 T16799 T16800 arg1Of generated,by
R12943 T16803 T16801 arg1Of transfection,calcium
R12944 T16803 T16802 arg1Of transfection,phosphate
R12945 T16803 T16804 arg1Of transfection,of
R12946 T16803 T16807 arg1Of transfection,and
R12947 T16806 T16804 arg2Of cells,of
R12948 T16806 T16805 arg1Of cells,Phoenix
R12949 T16807 T16800 arg2Of and,by
R12950 T16808 T16807 arg2Of concentration,and
R12951 T16808 T16809 arg1Of concentration,by
R12952 T16808 T16812 arg1Of concentration,at
R12953 T16811 T16809 arg2Of centrifugation,by
R12954 T16811 T16810 arg1Of centrifugation,overnight
R12955 T16814 T16812 arg2Of g,at
R12956 T16814 T16813 arg1Of g,"6,000"
R12957 T16817 T16815 arg2Of h,At
R12958 T16817 T16816 arg1Of h,∼42
R12959 T16817 T16818 arg1Of h,after
R12960 T16822 T16818 arg2Of activation,after
R12961 T16822 T16819 arg1Of activation,the
R12962 T16822 T16820 arg1Of activation,initial
R12963 T16822 T16821 arg1Of activation,TCR
R12964 T16822 T16823 arg1Of activation,of
R12965 T16822 T16828 arg1Of activation,per
R12966 T16827 T16823 arg2Of cells,of
R12967 T16827 T16824 arg1Of cells,106
R12968 T16827 T16825 arg1Of cells,CD8+
R12969 T16827 T16826 arg1Of cells,T
R12970 T16829 T16828 arg2Of well,per
R12971 T16829 T16830 arg1Of well,in
R12972 T16832 T16830 arg2Of plates,in
R12973 T16832 T16831 arg1Of plates,12-well
R12974 T16836 T16834 arg1Of media,the
R12975 T16836 T16835 arg1Of media,culture
R12976 T16836 T16837 arg1Of media,was
R12977 T16836 T16838 arg2Of media,removed
R12978 T16836 T16840 arg2Of media,replaced
R12979 T16838 T16839 arg1Of removed,and
R12980 T16839 T16815 arg1Of and,At
R12981 T16839 T16833 arg1Of and,","
R12982 T16839 T16837 arg2Of and,was
R12983 T16840 T16839 arg2Of replaced,and
R12984 T16840 T16841 arg1Of replaced,with
R12985 T16843 T16841 arg2Of media,with
R12986 T16843 T16842 arg1Of media,complete
R12987 T16843 T16844 arg2Of media,supplemented
R12988 T16844 T16845 arg1Of supplemented,with
R12989 T16848 T16845 arg2Of polybrene,with
R12990 T16848 T16846 arg1Of polybrene,8
R12991 T16848 T16847 arg1Of polybrene,μg/ml
R12992 T16848 T16849 arg1Of polybrene,containing
R12993 T16850 T16851 arg1Of fresh,plus
R12994 T16852 T16851 arg2Of concentrated,plus
R12995 T16853 T16849 arg2Of virus,containing
R12996 T16853 T16850 arg1Of virus,fresh
R12997 T16853 T16852 arg1Of virus,concentrated
R12998 T16855 T16854 arg1Of plates,The
R12999 T16855 T16856 arg1Of plates,were
R13000 T16855 T16857 arg2Of plates,centrifuged
R13001 T16855 T16867 arg1Of plates,returning
R13002 T16857 T16856 arg2Of centrifuged,were
R13003 T16857 T16858 arg1Of centrifuged,at
R13004 T16857 T16861 arg1Of centrifuged,for
R13005 T16857 T16864 arg1Of centrifuged,at
R13006 T16857 T16866 arg1Of centrifuged,before
R13007 T16860 T16858 arg2Of g,at
R13008 T16860 T16859 arg1Of g,700
R13009 T16863 T16861 arg2Of h,for
R13010 T16863 T16862 arg1Of h,1
R13011 T16865 T16864 arg2Of RT,at
R13012 T16867 T16866 arg2Of returning,before
R13013 T16867 T16868 arg1Of returning,to
R13014 T16867 T16870 arg1Of returning,for
R13015 T16869 T16868 arg2Of 37°C,to
R13016 T16874 T16870 arg2Of h,for
R13017 T16874 T16871 arg1Of h,an
R13018 T16874 T16872 arg1Of h,additional
R13019 T16874 T16873 arg1Of h,5
R13020 T16876 T16875 arg1Of constructs,Retroviral
R13021 T16876 T16877 arg1Of constructs,for
R13022 T16876 T16885 arg1Of constructs,were
R13023 T16878 T16879 arg1Of Eomes-VP16,and
R13024 T16879 T16877 arg2Of and,for
R13025 T16884 T16879 arg2Of vector,and
R13026 T16884 T16880 arg1Of vector,the
R13027 T16884 T16881 arg1Of vector,MIG
R13028 T16884 T16882 arg1Of vector,control
R13029 T16884 T16883 arg1Of vector,empty
R13030 T16887 T16885 arg2Of gift,were
R13031 T16887 T16886 arg1Of gift,a
R13032 T16887 T16888 arg1Of gift,from
R13033 T16890 T16888 arg2Of Reiner,from
R13034 T16890 T16889 arg1Of Reiner,S.L.
R13035 T16890 T16891 arg1Of Reiner,(
R13036 T16890 T16900 arg1Of Reiner,(
R13037 T16892 T16891 arg2Of University,(
R13038 T16892 T16893 arg1Of University,of
R13039 T16892 T16895 arg1Of University,","
R13040 T16894 T16893 arg2Of Pennsylvania,of
R13041 T16896 T16895 arg2Of Philadelphia,","
R13042 T16896 T16897 arg1Of Philadelphia,","
R13043 T16898 T16897 arg2Of PA,","
R13044 T16899 T16891 arg3Of ),(
R13045 T16901 T16900 arg2Of 8,(
R13046 T16902 T16900 arg3Of ),(
R12934 T16794 T16793 arg1Of experiments,transduction
R12939 T16799 T16795 arg1Of generated,","

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T16656 0-10 NNP denotes Retroviral
T16657 11-23 NN denotes transduction
T16658 24-26 IN denotes of
T16659 27-34 JJ denotes primary
T16660 35-38 NNP denotes CD8
T16661 38-39 CD denotes +
T16662 40-41 NNP denotes T
T16663 42-47 NNS denotes cells
T16664 47-48 . denotes .
T16665 49-52 IN denotes For
T16666 53-65 NN denotes transduction
T16667 66-77 NNS denotes experiments
T16668 77-78 , denotes ,
T16669 79-84 JJ denotes viral
T16670 85-97 NNS denotes supernatants
T16671 98-102 VBD denotes were
T16672 103-112 VBN denotes generated
T16673 113-115 IN denotes by
T16674 116-123 NN denotes calcium
T16675 124-133 NN denotes phosphate
T16676 134-146 NN denotes transfection
T16677 147-149 IN denotes of
T16678 150-157 NNP denotes Phoenix
T16679 158-163 NNS denotes cells
T16680 164-167 CC denotes and
T16681 168-181 NN denotes concentration
T16682 182-184 IN denotes by
T16683 185-194 JJ denotes overnight
T16684 195-209 NN denotes centrifugation
T16685 210-212 IN denotes at
T16686 213-218 CD denotes 6,000
T16687 219-220 NN denotes g
T16688 220-221 . denotes .
T16689 222-224 IN denotes At
T16690 225-226 CD denotes ∼
T16691 226-228 CD denotes 42
T16692 229-230 NN denotes h
T16693 231-236 IN denotes after
T16694 237-240 DT denotes the
T16695 241-248 JJ denotes initial
T16696 249-252 NNP denotes TCR
T16697 253-263 NN denotes activation
T16698 264-266 IN denotes of
T16699 267-270 CD denotes 106
T16700 271-274 CD denotes CD8
T16701 274-275 NN denotes +
T16702 276-277 NNP denotes T
T16703 278-283 NNS denotes cells
T16721 388-392 IN denotes with
T16722 393-394 CD denotes 8
T16723 395-397 NN denotes μg
T16724 397-398 NN denotes /
T16725 398-400 NN denotes ml
T16726 401-410 NN denotes polybrene
T16727 411-421 VBG denotes containing
T16728 422-427 JJ denotes fresh
T16729 428-432 CC denotes plus
T16730 433-445 JJ denotes concentrated
T16731 446-451 NN denotes virus
T16732 451-452 . denotes .
T16733 453-456 DT denotes The
T16734 457-463 NNS denotes plates
T16735 464-468 VBD denotes were
T16736 469-480 VBN denotes centrifuged
T16737 481-483 IN denotes at
T16738 484-487 CD denotes 700
T16739 488-489 NN denotes g
T16740 490-493 IN denotes for
T16741 494-495 CD denotes 1
T16742 496-497 NN denotes h
T16743 498-500 IN denotes at
T16744 501-503 NNP denotes RT
T16745 504-510 IN denotes before
T16746 511-520 VBG denotes returning
T16747 521-523 TO denotes to
T16748 524-526 CD denotes 37
T16749 526-527 CD denotes °
T16750 527-528 NNP denotes C
T16751 529-532 IN denotes for
T16752 533-535 DT denotes an
T16753 536-546 JJ denotes additional
T16754 547-548 CD denotes 5
T16755 549-551 NN denotes h.
T16756 552-562 NNP denotes Retroviral
T16757 563-573 NNS denotes constructs
T16758 574-577 IN denotes for
T16759 578-588 NNP denotes Eomes-VP16
T16760 589-592 CC denotes and
T16761 593-596 DT denotes the
T16762 597-600 NNP denotes MIG
T16763 601-608 NN denotes control
T16764 609-614 JJ denotes empty
T16765 615-621 NN denotes vector
T16766 622-626 VBD denotes were
T16767 627-628 DT denotes a
T16768 629-633 NN denotes gift
T16769 634-638 IN denotes from
T16770 639-643 NNP denotes S.L.
T16771 644-650 NNP denotes Reiner
T16772 651-652 -LRB- denotes (
T16773 652-662 NNP denotes University
T16774 663-665 IN denotes of
T16775 666-678 NNP denotes Pennsylvania
T16776 678-679 , denotes ,
T16777 680-692 NNP denotes Philadelphia
T16778 692-693 , denotes ,
T16779 694-696 NNP denotes PA
T16780 696-697 -RRB- denotes )
T16781 698-699 -LRB- denotes (
T16782 699-700 CD denotes 8
T16783 700-701 -RRB- denotes )
T16784 701-702 . denotes .
T16704 284-287 IN denotes per
T16705 288-292 RB denotes well
T16706 293-295 IN denotes in
T16707 296-303 JJ denotes 12-well
T16708 304-310 NNS denotes plates
T16709 310-311 , denotes ,
T16710 312-315 DT denotes the
T16711 316-323 NN denotes culture
T16712 324-329 NNS denotes media
T16713 330-333 VBD denotes was
T16714 334-341 VBN denotes removed
T16715 342-345 CC denotes and
T16716 346-354 VBN denotes replaced
T16717 355-359 IN denotes with
T16718 360-368 JJ denotes complete
T16719 369-374 NNS denotes media
T16720 375-387 VBN denotes supplemented
R12799 T16656 T16657 compound Retroviral,transduction
R12800 T16657 T16657 ROOT transduction,transduction
R12801 T16658 T16657 prep of,transduction
R12802 T16659 T16663 amod primary,cells
R12803 T16660 T16663 nmod CD8,cells
R12804 T16661 T16663 nummod +,cells
R12805 T16662 T16663 compound T,cells
R12806 T16663 T16658 pobj cells,of
R12807 T16664 T16657 punct .,transduction
R12808 T16665 T16672 prep For,generated
R12809 T16666 T16667 compound transduction,experiments
R12810 T16667 T16665 pobj experiments,For
R12811 T16668 T16672 punct ",",generated
R12812 T16669 T16670 amod viral,supernatants
R12813 T16670 T16672 nsubjpass supernatants,generated
R12814 T16671 T16672 auxpass were,generated
R12815 T16672 T16672 ROOT generated,generated
R12816 T16673 T16672 prep by,generated
R12817 T16674 T16676 compound calcium,transfection
R12818 T16675 T16676 compound phosphate,transfection
R12819 T16676 T16673 pobj transfection,by
R12820 T16677 T16676 prep of,transfection
R12821 T16678 T16679 compound Phoenix,cells
R12822 T16679 T16677 pobj cells,of
R12823 T16680 T16679 cc and,cells
R12824 T16681 T16676 conj concentration,transfection
R12825 T16682 T16681 prep by,concentration
R12826 T16683 T16684 amod overnight,centrifugation
R12827 T16684 T16682 pobj centrifugation,by
R12828 T16685 T16672 prep at,generated
R12829 T16686 T16687 nummod "6,000",g
R12830 T16687 T16685 pobj g,at
R12831 T16688 T16672 punct .,generated
R12832 T16689 T16714 prep At,removed
R12833 T16690 T16714 nsubjpass ∼,removed
R12834 T16691 T16692 nummod 42,h
R12835 T16692 T16693 npadvmod h,after
R12836 T16693 T16690 prep after,∼
R12837 T16694 T16697 det the,activation
R12838 T16695 T16697 amod initial,activation
R12839 T16696 T16697 compound TCR,activation
R12840 T16697 T16693 pobj activation,after
R12841 T16698 T16697 prep of,activation
R12842 T16699 T16703 nummod 106,cells
R12843 T16700 T16703 nummod CD8,cells
R12844 T16701 T16703 compound +,cells
R12845 T16702 T16703 compound T,cells
R12846 T16703 T16698 pobj cells,of
R12847 T16704 T16703 prep per,cells
R12848 T16705 T16706 advmod well,in
R12849 T16706 T16704 prep in,per
R12850 T16707 T16708 amod 12-well,plates
R12851 T16708 T16706 pobj plates,in
R12852 T16709 T16714 punct ",",removed
R12853 T16710 T16712 det the,media
R12854 T16711 T16712 compound culture,media
R12855 T16712 T16714 nsubjpass media,removed
R12856 T16713 T16714 auxpass was,removed
R12857 T16714 T16714 ROOT removed,removed
R12858 T16715 T16714 cc and,removed
R12859 T16716 T16714 conj replaced,removed
R12860 T16717 T16716 prep with,replaced
R12861 T16718 T16719 amod complete,media
R12862 T16719 T16717 pobj media,with
R12863 T16720 T16714 conj supplemented,removed
R12864 T16721 T16720 prep with,supplemented
R12865 T16722 T16723 nummod 8,μg
R12866 T16723 T16726 nmod μg,polybrene
R12867 T16724 T16726 nmod /,polybrene
R12868 T16725 T16726 compound ml,polybrene
R12869 T16726 T16721 pobj polybrene,with
R12870 T16727 T16726 acl containing,polybrene
R12871 T16728 T16731 amod fresh,virus
R12872 T16729 T16728 cc plus,fresh
R12873 T16730 T16728 conj concentrated,fresh
R12874 T16731 T16727 dobj virus,containing
R12875 T16732 T16714 punct .,removed
R12876 T16733 T16734 det The,plates
R12877 T16734 T16736 nsubjpass plates,centrifuged
R12878 T16735 T16736 auxpass were,centrifuged
R12879 T16736 T16736 ROOT centrifuged,centrifuged
R12880 T16737 T16736 prep at,centrifuged
R12881 T16738 T16739 nummod 700,g
R12882 T16739 T16737 pobj g,at
R12883 T16740 T16736 prep for,centrifuged
R12884 T16741 T16742 nummod 1,h
R12885 T16742 T16740 pobj h,for
R12886 T16743 T16742 prep at,h
R12887 T16744 T16743 pobj RT,at
R12888 T16745 T16736 prep before,centrifuged
R12889 T16746 T16745 pcomp returning,before
R12890 T16747 T16746 prep to,returning
R12891 T16748 T16749 compound 37,°
R12892 T16749 T16750 nummod °,C
R12893 T16750 T16747 pobj C,to
R12894 T16751 T16746 prep for,returning
R12895 T16752 T16757 det an,constructs
R12896 T16753 T16757 amod additional,constructs
R12897 T16754 T16755 nummod 5,h.
R12898 T16755 T16756 compound h.,Retroviral
R12899 T16756 T16757 compound Retroviral,constructs
R12900 T16757 T16751 pobj constructs,for
R12901 T16758 T16757 prep for,constructs
R12902 T16759 T16758 pobj Eomes-VP16,for
R12903 T16760 T16759 cc and,Eomes-VP16
R12904 T16761 T16765 det the,vector
R12905 T16762 T16763 compound MIG,control
R12906 T16763 T16765 nmod control,vector
R12907 T16764 T16765 amod empty,vector
R12908 T16765 T16759 conj vector,Eomes-VP16
R12909 T16766 T16736 auxpass were,centrifuged
R12910 T16767 T16768 det a,gift
R12911 T16768 T16736 nsubjpass gift,centrifuged
R12912 T16769 T16768 prep from,gift
R12913 T16770 T16771 compound S.L.,Reiner
R12914 T16771 T16769 pobj Reiner,from
R12915 T16772 T16771 punct (,Reiner
R12916 T16773 T16771 appos University,Reiner
R12917 T16774 T16773 prep of,University
R12918 T16775 T16774 pobj Pennsylvania,of
R12919 T16776 T16775 punct ",",Pennsylvania
R12920 T16777 T16775 conj Philadelphia,Pennsylvania
R12921 T16778 T16777 punct ",",Philadelphia
R12922 T16779 T16777 conj PA,Philadelphia
R12923 T16780 T16771 punct ),Reiner
R12924 T16781 T16771 punct (,Reiner
R12925 T16782 T16771 appos 8,Reiner
R12926 T16783 T16771 punct ),Reiner
R12927 T16784 T16736 punct .,centrifuged

GO-BP

Id Subject Object Predicate Lexical cue
T16652 11-23 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T16653 53-65 http://purl.obolibrary.org/obo/GO_0009293 denotes transduction
T16654 249-252 http://purl.obolibrary.org/obo/GO_0006283 denotes TCR
T16655 501-503 http://purl.obolibrary.org/obo/GO_0003964 denotes RT

GO-MF

Id Subject Object Predicate Lexical cue
T16913 501-503 http://purl.obolibrary.org/obo/GO_0003964 denotes RT

GO-CC

Id Subject Object Predicate Lexical cue
T16914 42-47 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16915 158-163 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16916 278-283 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T16917 249-252 http://purl.obolibrary.org/obo/GO_0042101 denotes TCR

sentences

Id Subject Object Predicate Lexical cue
T16647 0-48 Sentence denotes Retroviral transduction of primary CD8+ T cells.
T16648 49-221 Sentence denotes For transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g.
T16649 222-452 Sentence denotes At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus.
T16650 453-551 Sentence denotes The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h.
T16651 552-702 Sentence denotes Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L. Reiner (University of Pennsylvania, Philadelphia, PA) (8).
T182 0-48 Sentence denotes Retroviral transduction of primary CD8+ T cells.
T183 49-221 Sentence denotes For transduction experiments, viral supernatants were generated by calcium phosphate transfection of Phoenix cells and concentration by overnight centrifugation at 6,000 g.
T184 222-452 Sentence denotes At ∼42 h after the initial TCR activation of 106 CD8+ T cells per well in 12-well plates, the culture media was removed and replaced with complete media supplemented with 8 μg/ml polybrene containing fresh plus concentrated virus.
T185 453-551 Sentence denotes The plates were centrifuged at 700 g for 1 h at RT before returning to 37°C for an additional 5 h.
T186 552-643 Sentence denotes Retroviral constructs for Eomes-VP16 and the MIG control empty vector were a gift from S.L.
T187 644-702 Sentence denotes Reiner (University of Pennsylvania, Philadelphia, PA) (8).

events-check-again

Id Subject Object Predicate Lexical cue
T16929 578-588 Protein denotes Eomes-VP16
T16928 271-274 Protein denotes CD8

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T16930 35-39 Protein denotes CD8+
T16931 249-252 Protein denotes TCR
T16932 271-274 Protein denotes CD8
T16933 578-583 Protein denotes Eomes
T16934 584-588 Protein denotes VP16

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T16645 271-274 Protein denotes CD8
T16646 578-588 Protein denotes Eomes-VP16

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T16903 35-38 P10966 denotes CD8
T16904 35-38 P01732 denotes CD8
T16905 271-274 P10966 denotes CD8
T16906 271-274 P01732 denotes CD8
T16907 578-583 O95936 denotes Eomes

test2

Id Subject Object Predicate Lexical cue
T16643 271-274 Protein denotes CD8
T16644 578-588 Protein denotes Eomes-VP16