PMC:2626671 / 29155-29702 JSONTXT

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    pmc-enju-pas

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cytotoxicity assay.\nTo measure cytotoxicity, EL4 thymoma target cells were loaded with 0 or 1 μM Gp33 peptide for 2 h before a 2-h coincubation with P14 CD8+ T cells at the effector-to-target ratios indicated in the figures in 96-well round-bottom plates. After the coincubation period, cells were stained with Annexin V–FITC and anti-CD8–allophycocyanin. Data analysis was performed with FlowJo software (Tree Star, Inc.); EL4 target cells (CD8-negative events) were gated, and the percentage of Annexin V+ target cells was determined."}

    GO-CC

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    sentences

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    events-check-again

    {"project":"events-check-again","denotations":[{"id":"T16362","span":{"begin":164,"end":167},"obj":"Protein"},{"id":"T16363","span":{"begin":322,"end":331},"obj":"Protein"},{"id":"T16364","span":{"begin":346,"end":349},"obj":"Protein"},{"id":"T16365","span":{"begin":453,"end":456},"obj":"Protein"},{"id":"T16366","span":{"begin":508,"end":517},"obj":"Protein"}],"text":"FACS-based cytotoxicity assay.\nTo measure cytotoxicity, EL4 thymoma target cells were loaded with 0 or 1 μM Gp33 peptide for 2 h before a 2-h coincubation with P14 CD8+ T cells at the effector-to-target ratios indicated in the figures in 96-well round-bottom plates. After the coincubation period, cells were stained with Annexin V–FITC and anti-CD8–allophycocyanin. Data analysis was performed with FlowJo software (Tree Star, Inc.); EL4 target cells (CD8-negative events) were gated, and the percentage of Annexin V+ target cells was determined."}

    bionlp-st-ge-2016-reference-tees

    {"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T16367","span":{"begin":56,"end":59},"obj":"Protein"},{"id":"T16368","span":{"begin":105,"end":120},"obj":"Protein"},{"id":"T16369","span":{"begin":160,"end":163},"obj":"Protein"},{"id":"T16370","span":{"begin":346,"end":349},"obj":"Protein"},{"id":"T16371","span":{"begin":350,"end":365},"obj":"Protein"},{"id":"T16372","span":{"begin":453,"end":456},"obj":"Protein"}],"text":"FACS-based cytotoxicity assay.\nTo measure cytotoxicity, EL4 thymoma target cells were loaded with 0 or 1 μM Gp33 peptide for 2 h before a 2-h coincubation with P14 CD8+ T cells at the effector-to-target ratios indicated in the figures in 96-well round-bottom plates. After the coincubation period, cells were stained with Annexin V–FITC and anti-CD8–allophycocyanin. Data analysis was performed with FlowJo software (Tree Star, Inc.); EL4 target cells (CD8-negative events) were gated, and the percentage of Annexin V+ target cells was determined."}

    bionlp-st-ge-2016-reference

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    bionlp-st-ge-2016-uniprot

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    test2

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