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pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

UBERON-AE

{"project":"UBERON-AE","denotations":[{"id":"T14353","span":{"begin":246,"end":252},"obj":"http://purl.obolibrary.org/obo/UBERON_0002106"},{"id":"T14354","span":{"begin":257,"end":262},"obj":"http://purl.obolibrary.org/obo/UBERON_0002391"},{"id":"T14355","span":{"begin":257,"end":267},"obj":"http://purl.obolibrary.org/obo/UBERON_0000029"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

GO-BP

{"project":"GO-BP","denotations":[{"id":"T14374","span":{"begin":90,"end":93},"obj":"http://purl.obolibrary.org/obo/GO_0006283"},{"id":"T14375","span":{"begin":853,"end":856},"obj":"http://purl.obolibrary.org/obo/GO_0006283"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T14827","span":{"begin":40,"end":45},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14828","span":{"begin":54,"end":59},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14829","span":{"begin":268,"end":273},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14830","span":{"begin":282,"end":287},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14831","span":{"begin":623,"end":628},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14832","span":{"begin":650,"end":655},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14833","span":{"begin":825,"end":830},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14834","span":{"begin":914,"end":919},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14835","span":{"begin":986,"end":991},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14836","span":{"begin":1031,"end":1036},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T14837","span":{"begin":90,"end":93},"obj":"http://purl.obolibrary.org/obo/GO_0042101"},{"id":"T14838","span":{"begin":853,"end":856},"obj":"http://purl.obolibrary.org/obo/GO_0042101"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

sentences

{"project":"sentences","denotations":[{"id":"T14376","span":{"begin":0,"end":46},"obj":"Sentence"},{"id":"T14377","span":{"begin":47,"end":274},"obj":"Sentence"},{"id":"T14378","span":{"begin":275,"end":383},"obj":"Sentence"},{"id":"T14379","span":{"begin":384,"end":812},"obj":"Sentence"},{"id":"T14380","span":{"begin":813,"end":966},"obj":"Sentence"},{"id":"T14381","span":{"begin":967,"end":1103},"obj":"Sentence"},{"id":"T157","span":{"begin":0,"end":46},"obj":"Sentence"},{"id":"T158","span":{"begin":47,"end":274},"obj":"Sentence"},{"id":"T159","span":{"begin":275,"end":383},"obj":"Sentence"},{"id":"T160","span":{"begin":384,"end":589},"obj":"Sentence"},{"id":"T161","span":{"begin":590,"end":812},"obj":"Sentence"},{"id":"T162","span":{"begin":813,"end":966},"obj":"Sentence"},{"id":"T163","span":{"begin":967,"end":1103},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

events-check-again

{"project":"events-check-again","denotations":[{"id":"T14884","span":{"begin":47,"end":50},"obj":"Protein"},{"id":"T14885","span":{"begin":76,"end":80},"obj":"Protein"},{"id":"T14886","span":{"begin":132,"end":137},"obj":"Protein"},{"id":"T14887","span":{"begin":275,"end":278},"obj":"Protein"},{"id":"T14888","span":{"begin":293,"end":298},"obj":"Protein"},{"id":"T14889","span":{"begin":616,"end":619},"obj":"Protein"},{"id":"T14890","span":{"begin":714,"end":717},"obj":"Protein"},{"id":"T14891","span":{"begin":740,"end":744},"obj":"Protein"},{"id":"T14892","span":{"begin":1096,"end":1102},"obj":"Protein"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T14893","span":{"begin":33,"end":37},"obj":"Protein"},{"id":"T14894","span":{"begin":47,"end":50},"obj":"Protein"},{"id":"T14895","span":{"begin":86,"end":93},"obj":"Protein"},{"id":"T14896","span":{"begin":132,"end":137},"obj":"Protein"},{"id":"T14897","span":{"begin":275,"end":279},"obj":"Protein"},{"id":"T14898","span":{"begin":293,"end":298},"obj":"Protein"},{"id":"T14899","span":{"begin":502,"end":516},"obj":"Protein"},{"id":"T14900","span":{"begin":616,"end":620},"obj":"Protein"},{"id":"T14901","span":{"begin":709,"end":717},"obj":"Protein"},{"id":"T14902","span":{"begin":719,"end":729},"obj":"Protein"},{"id":"T14903","span":{"begin":740,"end":744},"obj":"Protein"},{"id":"T14904","span":{"begin":795,"end":811},"obj":"Protein"},{"id":"T14905","span":{"begin":853,"end":856},"obj":"Protein"},{"id":"T14906","span":{"begin":1096,"end":1102},"obj":"Protein"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

bionlp-st-ge-2016-reference

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T14365","span":{"begin":47,"end":50},"obj":"Protein"},{"id":"T14366","span":{"begin":76,"end":80},"obj":"Protein"},{"id":"T14367","span":{"begin":132,"end":137},"obj":"Protein"},{"id":"T14368","span":{"begin":275,"end":278},"obj":"Protein"},{"id":"T14369","span":{"begin":293,"end":298},"obj":"Protein"},{"id":"T14370","span":{"begin":616,"end":619},"obj":"Protein"},{"id":"T14371","span":{"begin":714,"end":717},"obj":"Protein"},{"id":"T14372","span":{"begin":740,"end":744},"obj":"Protein"},{"id":"T14373","span":{"begin":1096,"end":1102},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T14806","span":{"begin":33,"end":36},"obj":"P01732"},{"id":"T14807","span":{"begin":33,"end":36},"obj":"P10966"},{"id":"T14808","span":{"begin":47,"end":50},"obj":"P10966"},{"id":"T14809","span":{"begin":47,"end":50},"obj":"P01732"},{"id":"T14810","span":{"begin":132,"end":137},"obj":"Q9UL17"},{"id":"T14811","span":{"begin":275,"end":278},"obj":"P01732"},{"id":"T14812","span":{"begin":275,"end":278},"obj":"P10966"},{"id":"T14813","span":{"begin":293,"end":298},"obj":"Q13761"},{"id":"T14814","span":{"begin":616,"end":619},"obj":"P10966"},{"id":"T14815","span":{"begin":616,"end":619},"obj":"P01732"},{"id":"T14816","span":{"begin":714,"end":717},"obj":"P04234"},{"id":"T14817","span":{"begin":714,"end":717},"obj":"P20963"},{"id":"T14818","span":{"begin":714,"end":717},"obj":"P09693"},{"id":"T14819","span":{"begin":714,"end":717},"obj":"P07766"},{"id":"T14820","span":{"begin":740,"end":744},"obj":"P10747"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

test2

{"project":"test2","denotations":[{"id":"T14356","span":{"begin":47,"end":50},"obj":"Protein"},{"id":"T14357","span":{"begin":76,"end":80},"obj":"Protein"},{"id":"T14358","span":{"begin":132,"end":137},"obj":"Protein"},{"id":"T14359","span":{"begin":275,"end":278},"obj":"Protein"},{"id":"T14360","span":{"begin":293,"end":298},"obj":"Protein"},{"id":"T14361","span":{"begin":616,"end":619},"obj":"Protein"},{"id":"T14362","span":{"begin":714,"end":717},"obj":"Protein"},{"id":"T14363","span":{"begin":740,"end":744},"obj":"Protein"},{"id":"T14364","span":{"begin":1096,"end":1102},"obj":"Protein"}],"text":"Isolation and culture of primary CD8+ T cells.\nCD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (\u003e95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2."}

PMC:2626671 / 26310-27413 JSONTXT

Annnotations TAB JSON ListView MergeView

PMC:2626671 / 26310-27413 JSON TXT