PMC:2626671 / 26310-27413
Annnotations
pmc-enju-pas
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14607 | 0-9 | NN | denotes | Isolation |
T14608 | 10-13 | CC | denotes | and |
T14609 | 14-21 | NN | denotes | culture |
T14610 | 22-24 | IN | denotes | of |
T14611 | 25-32 | JJ | denotes | primary |
T14612 | 33-37 | JJ | denotes | CD8+ |
T14613 | 38-39 | NN | denotes | T |
T14614 | 40-45 | NN | denotes | cells |
T14615 | 47-51 | JJ | denotes | CD8+ |
T14616 | 52-53 | NN | denotes | T |
T14617 | 54-59 | NN | denotes | cells |
T14618 | 60-64 | IN | denotes | from |
T14619 | 65-75 | JJ | denotes | 4–8-wk-old |
T14620 | 76-83 | NN | denotes | Tcra−/− |
T14621 | 84-85 | NN | denotes | × |
T14622 | 86-89 | NN | denotes | P14 |
T14623 | 90-93 | NN | denotes | TCR |
T14624 | 94-104 | JJ | denotes | transgenic |
T14625 | 105-106 | -LRB- | denotes | ( |
T14626 | 106-113 | JJ | denotes | Taconic |
T14627 | 113-114 | -RRB- | denotes | ) |
T14628 | 114-115 | -COMMA- | denotes | , |
T14629 | 116-124 | NN | denotes | C57BL/6J |
T14630 | 125-127 | NNP | denotes | WT |
T14631 | 127-128 | -COMMA- | denotes | , |
T14632 | 129-131 | CC | denotes | or |
T14633 | 132-140 | NN | denotes | Tbx21−/− |
T14634 | 141-142 | -LRB- | denotes | ( |
T14635 | 142-145 | DT | denotes | The |
T14636 | 146-153 | NNP | denotes | Jackson |
T14637 | 154-164 | NNP | denotes | Laboratory |
T14638 | 164-165 | -RRB- | denotes | ) |
T14639 | 166-170 | NN | denotes | mice |
T14640 | 171-175 | VB | denotes | were |
T14641 | 176-184 | VB | denotes | purified |
T14642 | 185-186 | -LRB- | denotes | ( |
T14643 | 186-187 | SYM | denotes | > |
T14644 | 187-189 | CD | denotes | 95 |
T14645 | 189-190 | NN | denotes | % |
T14646 | 191-197 | NN | denotes | purity |
T14647 | 197-198 | -RRB- | denotes | ) |
T14648 | 199-201 | IN | denotes | by |
T14649 | 202-210 | JJ | denotes | negative |
T14650 | 211-220 | NN | denotes | selection |
T14651 | 221-222 | -LRB- | denotes | ( |
T14652 | 222-232 | NN | denotes | Invitrogen |
T14653 | 232-233 | -RRB- | denotes | ) |
T14654 | 234-238 | IN | denotes | from |
T14655 | 239-245 | VB | denotes | pooled |
T14656 | 246-252 | NN | denotes | spleen |
T14657 | 253-256 | CC | denotes | and |
T14658 | 257-262 | NN | denotes | lymph |
T14659 | 263-267 | NN | denotes | node |
T14660 | 268-273 | NN | denotes | cells |
T14661 | 275-279 | JJ | denotes | CD8+ |
T14662 | 280-281 | NN | denotes | T |
T14663 | 282-287 | NN | denotes | cells |
T14664 | 288-292 | IN | denotes | from |
T14665 | 293-301 | NN | denotes | Runx3−/− |
T14666 | 302-306 | NN | denotes | mice |
T14667 | 307-309 | IN | denotes | on |
T14668 | 310-313 | DT | denotes | the |
T14669 | 314-317 | NN | denotes | ICR |
T14670 | 318-328 | NN | denotes | background |
T14671 | 329-333 | VB | denotes | were |
T14672 | 334-342 | VB | denotes | purified |
T14673 | 343-345 | IN | denotes | by |
T14674 | 346-354 | JJ | denotes | positive |
T14675 | 355-364 | NN | denotes | selection |
T14676 | 365-366 | -LRB- | denotes | ( |
T14677 | 366-374 | NNP | denotes | Miltenyi |
T14678 | 375-381 | NNP | denotes | Biotec |
T14679 | 381-382 | -RRB- | denotes | ) |
T14680 | 384-387 | DT | denotes | All |
T14681 | 388-392 | NN | denotes | mice |
T14682 | 393-397 | VB | denotes | were |
T14683 | 398-408 | VB | denotes | maintained |
T14684 | 409-411 | IN | denotes | in |
T14685 | 412-420 | JJ | denotes | specific |
T14686 | 421-434 | JJ | denotes | pathogen-free |
T14687 | 435-442 | NN | denotes | barrier |
T14688 | 443-453 | NN | denotes | facilities |
T14689 | 454-457 | CC | denotes | and |
T14690 | 458-462 | VB | denotes | used |
T14691 | 463-472 | VB | denotes | according |
T14692 | 473-475 | TO | denotes | to |
T14693 | 476-485 | NN | denotes | protocols |
T14694 | 486-494 | VB | denotes | approved |
T14695 | 495-497 | IN | denotes | by |
T14696 | 498-501 | DT | denotes | the |
T14697 | 502-508 | NNP | denotes | Immune |
T14698 | 509-516 | NNP | denotes | Disease |
T14699 | 517-526 | NNP | denotes | Institute |
T14700 | 527-530 | CC | denotes | and |
T14701 | 531-534 | DT | denotes | the |
T14702 | 535-542 | NNP | denotes | Harvard |
T14703 | 543-550 | NNP | denotes | Medical |
T14704 | 551-557 | NNP | denotes | School |
T14705 | 558-564 | NNP | denotes | Animal |
T14706 | 565-569 | NNP | denotes | Care |
T14707 | 570-573 | CC | denotes | and |
T14708 | 574-577 | NNP | denotes | Use |
T14709 | 578-589 | NNP | denotes | Committees. |
T14710 | 590-593 | IN | denotes | For |
T14711 | 594-605 | NN | denotes | stimulation |
T14712 | 605-606 | -COMMA- | denotes | , |
T14713 | 607-615 | VB | denotes | purified |
T14714 | 616-620 | JJ | denotes | CD8+ |
T14715 | 621-622 | NN | denotes | T |
T14716 | 623-628 | NN | denotes | cells |
T14717 | 629-633 | VB | denotes | were |
T14718 | 634-642 | VB | denotes | cultured |
T14719 | 643-645 | IN | denotes | at |
T14720 | 646-649 | CD | denotes | 106 |
T14721 | 650-658 | NN | denotes | cells/ml |
T14722 | 659-660 | -LRB- | denotes | ( |
T14723 | 660-662 | CD | denotes | 10 |
T14724 | 663-665 | NN | denotes | ml |
T14725 | 665-666 | -RRB- | denotes | ) |
T14726 | 667-669 | IN | denotes | in |
T14727 | 670-673 | NN | denotes | T25 |
T14728 | 674-680 | NN | denotes | flasks |
T14729 | 681-687 | VB | denotes | coated |
T14730 | 688-692 | IN | denotes | with |
T14731 | 693-694 | CD | denotes | 1 |
T14732 | 695-700 | NN | denotes | μg/ml |
T14733 | 701-705 | DT | denotes | each |
T14734 | 706-708 | IN | denotes | of |
T14735 | 709-717 | NN | denotes | anti-CD3 |
T14736 | 718-719 | -LRB- | denotes | ( |
T14737 | 719-724 | NN | denotes | clone |
T14738 | 725-729 | NN | denotes | 2C11 |
T14739 | 729-730 | -RRB- | denotes | ) |
T14740 | 731-734 | CC | denotes | and |
T14741 | 735-744 | JJ | denotes | anti-CD28 |
T14742 | 745-746 | -LRB- | denotes | ( |
T14743 | 746-751 | NN | denotes | clone |
T14744 | 752-757 | CD | denotes | 37.51 |
T14745 | 757-758 | -RRB- | denotes | ) |
T14746 | 759-761 | IN | denotes | by |
T14747 | 762-774 | NN | denotes | pretreatment |
T14748 | 775-779 | IN | denotes | with |
T14749 | 780-783 | CD | denotes | 300 |
T14750 | 784-789 | NN | denotes | μg/ml |
T14751 | 790-794 | NN | denotes | goat |
T14752 | 795-807 | NN | denotes | anti–hamster |
T14753 | 808-811 | NN | denotes | IgG |
T14754 | 813-818 | IN | denotes | After |
T14755 | 819-821 | CD | denotes | 48 |
T14756 | 822-823 | NN | denotes | h |
T14757 | 823-824 | -COMMA- | denotes | , |
T14758 | 825-830 | NN | denotes | cells |
T14759 | 831-835 | VB | denotes | were |
T14760 | 836-843 | VB | denotes | removed |
T14761 | 844-848 | IN | denotes | from |
T14762 | 849-852 | DT | denotes | the |
T14763 | 853-856 | NN | denotes | TCR |
T14764 | 857-868 | NN | denotes | stimulation |
T14765 | 869-872 | CC | denotes | and |
T14766 | 873-883 | VB | denotes | recultured |
T14767 | 884-886 | IN | denotes | at |
T14768 | 887-888 | DT | denotes | a |
T14769 | 889-902 | NN | denotes | concentration |
T14770 | 903-905 | IN | denotes | of |
T14771 | 906-907 | CD | denotes | 5 |
T14772 | 908-909 | IN | denotes | × |
T14773 | 910-913 | CD | denotes | 105 |
T14774 | 914-922 | NN | denotes | cells/ml |
T14775 | 923-925 | IN | denotes | in |
T14776 | 926-931 | NN | denotes | media |
T14777 | 932-944 | VB | denotes | supplemented |
T14778 | 945-949 | IN | denotes | with |
T14779 | 950-953 | CD | denotes | 100 |
T14780 | 954-958 | NN | denotes | U/ml |
T14781 | 959-965 | NN | denotes | rhIL-2 |
T14782 | 967-972 | DT | denotes | Every |
T14783 | 973-975 | CD | denotes | 24 |
T14784 | 976-977 | NN | denotes | h |
T14785 | 977-978 | -COMMA- | denotes | , |
T14786 | 979-985 | JJ | denotes | viable |
T14787 | 986-991 | NN | denotes | cells |
T14788 | 992-996 | VB | denotes | were |
T14789 | 997-1004 | VB | denotes | counted |
T14790 | 1005-1008 | CC | denotes | and |
T14791 | 1009-1019 | VB | denotes | readjusted |
T14792 | 1020-1022 | TO | denotes | to |
T14793 | 1023-1024 | CD | denotes | 5 |
T14794 | 1025-1026 | IN | denotes | × |
T14795 | 1027-1030 | CD | denotes | 105 |
T14796 | 1031-1039 | NN | denotes | cells/ml |
T14797 | 1040-1044 | IN | denotes | with |
T14798 | 1045-1050 | JJ | denotes | fresh |
T14799 | 1051-1056 | NN | denotes | media |
T14800 | 1057-1067 | VB | denotes | containing |
T14801 | 1068-1071 | DT | denotes | the |
T14802 | 1072-1085 | VB | denotes | corresponding |
T14803 | 1086-1092 | NN | denotes | amount |
T14804 | 1093-1095 | IN | denotes | of |
T14805 | 1096-1102 | NN | denotes | rhIL-2 |
R11380 | T14607 | T14608 | arg1Of | Isolation,and |
R11381 | T14608 | T14610 | arg1Of | and,of |
R11382 | T14609 | T14608 | arg2Of | culture,and |
R11383 | T14614 | T14610 | arg2Of | cells,of |
R11384 | T14614 | T14611 | arg1Of | cells,primary |
R11385 | T14614 | T14612 | arg1Of | cells,CD8+ |
R11386 | T14614 | T14613 | arg1Of | cells,T |
R11387 | T14617 | T14615 | arg1Of | cells,CD8+ |
R11388 | T14617 | T14616 | arg1Of | cells,T |
R11389 | T14617 | T14618 | arg1Of | cells,from |
R11390 | T14617 | T14624 | arg1Of | cells,transgenic |
R11391 | T14617 | T14628 | arg1Of | cells,"," |
R11392 | T14623 | T14618 | arg2Of | TCR,from |
R11393 | T14623 | T14619 | arg1Of | TCR,4–8-wk-old |
R11394 | T14623 | T14620 | arg1Of | TCR,Tcra−/− |
R11395 | T14623 | T14621 | arg1Of | TCR,× |
R11396 | T14623 | T14622 | arg1Of | TCR,P14 |
R11397 | T14624 | T14625 | arg1Of | transgenic,( |
R11398 | T14626 | T14625 | arg2Of | Taconic,( |
R11399 | T14627 | T14625 | arg3Of | ),( |
R11400 | T14628 | T14632 | arg1Of | ",",or |
R11401 | T14630 | T14628 | arg2Of | WT,"," |
R11402 | T14630 | T14629 | arg1Of | WT,C57BL/6J |
R11403 | T14632 | T14631 | arg1Of | or,"," |
R11404 | T14632 | T14640 | arg1Of | or,were |
R11405 | T14632 | T14641 | arg2Of | or,purified |
R11406 | T14633 | T14634 | arg1Of | Tbx21−/−,( |
R11407 | T14637 | T14634 | arg2Of | Laboratory,( |
R11408 | T14637 | T14635 | arg1Of | Laboratory,The |
R11409 | T14637 | T14636 | arg1Of | Laboratory,Jackson |
R11410 | T14638 | T14634 | arg3Of | ),( |
R11411 | T14639 | T14632 | arg2Of | mice,or |
R11412 | T14639 | T14633 | arg1Of | mice,Tbx21−/− |
R11413 | T14641 | T14640 | arg2Of | purified,were |
R11414 | T14641 | T14642 | arg1Of | purified,( |
R11415 | T14641 | T14648 | arg1Of | purified,by |
R11416 | T14641 | T14654 | arg1Of | purified,from |
R11417 | T14644 | T14643 | arg1Of | 95,> |
R11418 | T14644 | T14645 | arg1Of | 95,% |
R11419 | T14646 | T14642 | arg2Of | purity,( |
R11420 | T14646 | T14644 | arg1Of | purity,95 |
R11421 | T14647 | T14642 | arg3Of | ),( |
R11422 | T14650 | T14648 | arg2Of | selection,by |
R11423 | T14650 | T14649 | arg1Of | selection,negative |
R11424 | T14650 | T14651 | arg1Of | selection,( |
R11425 | T14652 | T14651 | arg2Of | Invitrogen,( |
R11426 | T14653 | T14651 | arg3Of | ),( |
R11427 | T14656 | T14657 | arg1Of | spleen,and |
R11428 | T14658 | T14657 | arg2Of | lymph,and |
R11429 | T14660 | T14654 | arg2Of | cells,from |
R11430 | T14660 | T14655 | arg2Of | cells,pooled |
R11431 | T14660 | T14656 | arg1Of | cells,spleen |
R11432 | T14660 | T14658 | arg1Of | cells,lymph |
R11433 | T14660 | T14659 | arg1Of | cells,node |
R11434 | T14663 | T14661 | arg1Of | cells,CD8+ |
R11435 | T14663 | T14662 | arg1Of | cells,T |
R11436 | T14663 | T14664 | arg1Of | cells,from |
R11437 | T14663 | T14671 | arg1Of | cells,were |
R11438 | T14663 | T14672 | arg2Of | cells,purified |
R11439 | T14666 | T14664 | arg2Of | mice,from |
R11440 | T14666 | T14665 | arg1Of | mice,Runx3−/− |
R11441 | T14666 | T14667 | arg1Of | mice,on |
R11442 | T14670 | T14667 | arg2Of | background,on |
R11443 | T14670 | T14668 | arg1Of | background,the |
R11444 | T14670 | T14669 | arg1Of | background,ICR |
R11445 | T14672 | T14671 | arg2Of | purified,were |
R11446 | T14675 | T14672 | arg1Of | selection,purified |
R11447 | T14675 | T14673 | arg2Of | selection,by |
R11448 | T14675 | T14674 | arg1Of | selection,positive |
R11449 | T14675 | T14676 | arg1Of | selection,( |
R11450 | T14678 | T14676 | arg2Of | Biotec,( |
R11451 | T14678 | T14677 | arg1Of | Biotec,Miltenyi |
R11452 | T14679 | T14676 | arg3Of | ),( |
R11453 | T14681 | T14680 | arg1Of | mice,All |
R11454 | T14681 | T14682 | arg1Of | mice,were |
R11455 | T14681 | T14683 | arg2Of | mice,maintained |
R11456 | T14681 | T14690 | arg2Of | mice,used |
R11457 | T14683 | T14684 | arg1Of | maintained,in |
R11458 | T14683 | T14689 | arg1Of | maintained,and |
R11459 | T14688 | T14684 | arg2Of | facilities,in |
R11460 | T14688 | T14685 | arg1Of | facilities,specific |
R11461 | T14688 | T14686 | arg1Of | facilities,pathogen-free |
R11462 | T14688 | T14687 | arg1Of | facilities,barrier |
R11463 | T14689 | T14682 | arg2Of | and,were |
R11464 | T14689 | T14712 | arg1Of | and,"," |
R11465 | T14690 | T14689 | arg2Of | used,and |
R11466 | T14690 | T14691 | arg1Of | used,according |
R11467 | T14690 | T14710 | arg1Of | used,For |
R11468 | T14692 | T14691 | arg2Of | to,according |
R11469 | T14693 | T14694 | arg2Of | protocols,approved |
R11470 | T14693 | T14700 | arg1Of | protocols,and |
R11471 | T14699 | T14694 | arg1Of | Institute,approved |
R11472 | T14699 | T14695 | arg2Of | Institute,by |
R11473 | T14699 | T14696 | arg1Of | Institute,the |
R11474 | T14699 | T14697 | arg1Of | Institute,Immune |
R11475 | T14699 | T14698 | arg1Of | Institute,Disease |
R11476 | T14700 | T14692 | arg2Of | and,to |
R11477 | T14706 | T14702 | arg1Of | Care,Harvard |
R11478 | T14706 | T14703 | arg1Of | Care,Medical |
R11479 | T14706 | T14704 | arg1Of | Care,School |
R11480 | T14706 | T14705 | arg1Of | Care,Animal |
R11481 | T14706 | T14707 | arg1Of | Care,and |
R11482 | T14708 | T14707 | arg2Of | Use,and |
R11483 | T14709 | T14700 | arg2Of | Committees.,and |
R11484 | T14709 | T14701 | arg1Of | Committees.,the |
R11485 | T14709 | T14706 | arg1Of | Committees.,Care |
R11486 | T14709 | T14708 | arg1Of | Committees.,Use |
R11487 | T14711 | T14710 | arg2Of | stimulation,For |
R11488 | T14716 | T14713 | arg2Of | cells,purified |
R11489 | T14716 | T14714 | arg1Of | cells,CD8+ |
R11490 | T14716 | T14715 | arg1Of | cells,T |
R11491 | T14716 | T14717 | arg1Of | cells,were |
R11492 | T14716 | T14718 | arg2Of | cells,cultured |
R11493 | T14718 | T14712 | arg2Of | cultured,"," |
R11494 | T14718 | T14717 | arg2Of | cultured,were |
R11495 | T14718 | T14719 | arg1Of | cultured,at |
R11496 | T14721 | T14719 | arg2Of | cells/ml,at |
R11497 | T14721 | T14720 | arg1Of | cells/ml,106 |
R11498 | T14721 | T14722 | arg1Of | cells/ml,( |
R11499 | T14721 | T14726 | arg1Of | cells/ml,in |
R11500 | T14724 | T14722 | arg2Of | ml,( |
R11501 | T14724 | T14723 | arg1Of | ml,10 |
R11502 | T14725 | T14722 | arg3Of | ),( |
R11503 | T14728 | T14726 | arg2Of | flasks,in |
R11504 | T14728 | T14727 | arg1Of | flasks,T25 |
R11505 | T14728 | T14729 | arg2Of | flasks,coated |
R11506 | T14729 | T14730 | arg1Of | coated,with |
R11507 | T14732 | T14730 | arg2Of | μg/ml,with |
R11508 | T14732 | T14731 | arg1Of | μg/ml,1 |
R11509 | T14732 | T14733 | arg1Of | μg/ml,each |
R11510 | T14732 | T14734 | arg1Of | μg/ml,of |
R11511 | T14735 | T14736 | arg1Of | anti-CD3,( |
R11512 | T14735 | T14740 | arg1Of | anti-CD3,and |
R11513 | T14738 | T14736 | arg2Of | 2C11,( |
R11514 | T14738 | T14737 | arg1Of | 2C11,clone |
R11515 | T14739 | T14736 | arg3Of | ),( |
R11516 | T14740 | T14734 | arg2Of | and,of |
R11517 | T14740 | T14746 | arg1Of | and,by |
R11518 | T14743 | T14740 | arg2Of | clone,and |
R11519 | T14743 | T14741 | arg1Of | clone,anti-CD28 |
R11520 | T14743 | T14742 | arg2Of | clone,( |
R11521 | T14743 | T14744 | arg1Of | clone,37.51 |
R11522 | T14745 | T14742 | arg3Of | ),( |
R11523 | T14747 | T14746 | arg2Of | pretreatment,by |
R11524 | T14747 | T14748 | arg1Of | pretreatment,with |
R11525 | T14753 | T14748 | arg2Of | IgG,with |
R11526 | T14753 | T14749 | arg1Of | IgG,300 |
R11527 | T14753 | T14750 | arg1Of | IgG,μg/ml |
R11528 | T14753 | T14751 | arg1Of | IgG,goat |
R11529 | T14753 | T14752 | arg1Of | IgG,anti–hamster |
R11530 | T14756 | T14754 | arg2Of | h,After |
R11531 | T14756 | T14755 | arg1Of | h,48 |
R11532 | T14758 | T14759 | arg1Of | cells,were |
R11533 | T14758 | T14760 | arg2Of | cells,removed |
R11534 | T14758 | T14766 | arg2Of | cells,recultured |
R11535 | T14760 | T14761 | arg1Of | removed,from |
R11536 | T14760 | T14765 | arg1Of | removed,and |
R11537 | T14764 | T14761 | arg2Of | stimulation,from |
R11538 | T14764 | T14762 | arg1Of | stimulation,the |
R11539 | T14764 | T14763 | arg1Of | stimulation,TCR |
R11540 | T14765 | T14754 | arg1Of | and,After |
R11541 | T14765 | T14757 | arg1Of | and,"," |
R11542 | T14765 | T14759 | arg2Of | and,were |
R11543 | T14766 | T14765 | arg2Of | recultured,and |
R11544 | T14766 | T14767 | arg1Of | recultured,at |
R11545 | T14769 | T14767 | arg2Of | concentration,at |
R11546 | T14769 | T14768 | arg1Of | concentration,a |
R11547 | T14769 | T14770 | arg1Of | concentration,of |
R11548 | T14769 | T14775 | arg1Of | concentration,in |
R11549 | T14773 | T14772 | arg1Of | 105,× |
R11550 | T14774 | T14770 | arg2Of | cells/ml,of |
R11551 | T14774 | T14771 | arg1Of | cells/ml,5 |
R11552 | T14774 | T14773 | arg1Of | cells/ml,105 |
R11553 | T14776 | T14775 | arg2Of | media,in |
R11554 | T14776 | T14777 | arg2Of | media,supplemented |
R11555 | T14777 | T14778 | arg1Of | supplemented,with |
R11556 | T14781 | T14778 | arg2Of | rhIL-2,with |
R11557 | T14781 | T14779 | arg1Of | rhIL-2,100 |
R11558 | T14781 | T14780 | arg1Of | rhIL-2,U/ml |
R11559 | T14784 | T14782 | arg1Of | h,Every |
R11560 | T14784 | T14783 | arg1Of | h,24 |
R11561 | T14787 | T14786 | arg1Of | cells,viable |
R11562 | T14787 | T14788 | arg1Of | cells,were |
R11563 | T14787 | T14789 | arg2Of | cells,counted |
R11564 | T14787 | T14791 | arg2Of | cells,readjusted |
R11565 | T14789 | T14790 | arg1Of | counted,and |
R11566 | T14790 | T14784 | arg1Of | and,h |
R11567 | T14790 | T14785 | arg1Of | and,"," |
R11568 | T14790 | T14788 | arg2Of | and,were |
R11569 | T14791 | T14790 | arg2Of | readjusted,and |
R11570 | T14791 | T14792 | arg1Of | readjusted,to |
R11571 | T14795 | T14794 | arg1Of | 105,× |
R11572 | T14796 | T14792 | arg2Of | cells/ml,to |
R11573 | T14796 | T14793 | arg1Of | cells/ml,5 |
R11574 | T14796 | T14795 | arg1Of | cells/ml,105 |
R11575 | T14796 | T14797 | arg1Of | cells/ml,with |
R11576 | T14799 | T14797 | arg2Of | media,with |
R11577 | T14799 | T14798 | arg1Of | media,fresh |
R11578 | T14799 | T14800 | arg1Of | media,containing |
R11579 | T14803 | T14800 | arg2Of | amount,containing |
R11580 | T14803 | T14801 | arg1Of | amount,the |
R11581 | T14803 | T14802 | arg1Of | amount,corresponding |
R11582 | T14803 | T14804 | arg1Of | amount,of |
R11583 | T14805 | T14804 | arg2Of | rhIL-2,of |
bionlp-st-ge-2016-spacy-parsed
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14382 | 0-9 | NN | denotes | Isolation |
T14383 | 10-13 | CC | denotes | and |
T14384 | 14-21 | NN | denotes | culture |
T14385 | 22-24 | IN | denotes | of |
T14386 | 25-32 | JJ | denotes | primary |
T14387 | 33-36 | NNP | denotes | CD8 |
T14388 | 36-37 | CD | denotes | + |
T14389 | 38-39 | NNP | denotes | T |
T14390 | 40-45 | NNS | denotes | cells |
T14391 | 45-46 | . | denotes | . |
T14392 | 47-50 | CD | denotes | CD8 |
T14393 | 50-51 | CD | denotes | + |
T14394 | 52-53 | NNP | denotes | T |
T14395 | 54-59 | NNS | denotes | cells |
T14396 | 60-64 | IN | denotes | from |
T14397 | 65-66 | CD | denotes | 4 |
T14398 | 67-75 | JJ | denotes | 8-wk-old |
T14399 | 76-80 | NNP | denotes | Tcra |
T14400 | 80-81 | FW | denotes | − |
T14401 | 81-82 | FW | denotes | / |
T14402 | 82-83 | FW | denotes | − |
T14403 | 84-85 | FW | denotes | × |
T14404 | 86-89 | FW | denotes | P14 |
T14405 | 90-93 | FW | denotes | TCR |
T14406 | 94-104 | FW | denotes | transgenic |
T14407 | 105-106 | -LRB- | denotes | ( |
T14408 | 106-113 | NNP | denotes | Taconic |
T14409 | 113-114 | -RRB- | denotes | ) |
T14410 | 114-115 | , | denotes | , |
T14411 | 116-124 | NNP | denotes | C57BL/6J |
T14412 | 125-127 | NNP | denotes | WT |
T14413 | 127-128 | , | denotes | , |
T14414 | 129-131 | CC | denotes | or |
T14415 | 132-137 | CD | denotes | Tbx21 |
T14416 | 137-138 | CD | denotes | − |
T14417 | 138-139 | NN | denotes | / |
T14418 | 139-140 | NN | denotes | − |
T14419 | 141-142 | -LRB- | denotes | ( |
T14420 | 142-145 | DT | denotes | The |
T14421 | 146-153 | NNP | denotes | Jackson |
T14422 | 154-164 | NNP | denotes | Laboratory |
T14423 | 164-165 | -RRB- | denotes | ) |
T14424 | 166-170 | NNS | denotes | mice |
T14425 | 171-175 | VBD | denotes | were |
T14426 | 176-184 | VBN | denotes | purified |
T14427 | 185-186 | -LRB- | denotes | ( |
T14428 | 186-187 | FW | denotes | > |
T14429 | 187-189 | CD | denotes | 95 |
T14430 | 189-190 | NN | denotes | % |
T14431 | 191-197 | NN | denotes | purity |
T14432 | 197-198 | -RRB- | denotes | ) |
T14433 | 199-201 | IN | denotes | by |
T14434 | 202-210 | JJ | denotes | negative |
T14435 | 211-220 | NN | denotes | selection |
T14436 | 221-222 | -LRB- | denotes | ( |
T14437 | 222-232 | NNP | denotes | Invitrogen |
T14438 | 232-233 | -RRB- | denotes | ) |
T14439 | 234-238 | IN | denotes | from |
T14440 | 239-245 | VBN | denotes | pooled |
T14441 | 246-252 | NN | denotes | spleen |
T14442 | 253-256 | CC | denotes | and |
T14443 | 257-262 | NN | denotes | lymph |
T14444 | 263-267 | NN | denotes | node |
T14445 | 268-273 | NNS | denotes | cells |
T14446 | 273-274 | . | denotes | . |
T14447 | 275-278 | CD | denotes | CD8 |
T14448 | 278-279 | CD | denotes | + |
T14449 | 280-281 | NNP | denotes | T |
T14450 | 282-287 | NNS | denotes | cells |
T14451 | 288-292 | IN | denotes | from |
T14452 | 293-298 | JJ | denotes | Runx3 |
T14453 | 298-299 | NN | denotes | − |
T14454 | 299-300 | NN | denotes | / |
T14455 | 300-301 | NN | denotes | − |
T14456 | 302-306 | NNS | denotes | mice |
T14457 | 307-309 | IN | denotes | on |
T14458 | 310-313 | DT | denotes | the |
T14459 | 314-317 | NNP | denotes | ICR |
T14460 | 318-328 | NN | denotes | background |
T14461 | 329-333 | VBD | denotes | were |
T14462 | 334-342 | VBN | denotes | purified |
T14463 | 343-345 | IN | denotes | by |
T14464 | 346-354 | JJ | denotes | positive |
T14465 | 355-364 | NN | denotes | selection |
T14466 | 365-366 | -LRB- | denotes | ( |
T14467 | 366-374 | NNP | denotes | Miltenyi |
T14468 | 375-381 | NNP | denotes | Biotec |
T14469 | 381-382 | -RRB- | denotes | ) |
T14470 | 382-383 | . | denotes | . |
T14471 | 384-387 | DT | denotes | All |
T14472 | 388-392 | NNS | denotes | mice |
T14473 | 393-397 | VBD | denotes | were |
T14474 | 398-408 | VBN | denotes | maintained |
T14475 | 409-411 | IN | denotes | in |
T14476 | 412-420 | JJ | denotes | specific |
T14477 | 421-434 | JJ | denotes | pathogen-free |
T14478 | 435-442 | NN | denotes | barrier |
T14479 | 443-453 | NNS | denotes | facilities |
T14480 | 454-457 | CC | denotes | and |
T14481 | 458-462 | VBN | denotes | used |
T14482 | 463-472 | VBG | denotes | according |
T14483 | 473-475 | TO | denotes | to |
T14484 | 476-485 | NNS | denotes | protocols |
T14485 | 486-494 | VBN | denotes | approved |
T14486 | 495-497 | IN | denotes | by |
T14487 | 498-501 | DT | denotes | the |
T14488 | 502-508 | NNP | denotes | Immune |
T14489 | 509-516 | NNP | denotes | Disease |
T14490 | 517-526 | NNP | denotes | Institute |
T14491 | 527-530 | CC | denotes | and |
T14492 | 531-534 | DT | denotes | the |
T14493 | 535-542 | NNP | denotes | Harvard |
T14494 | 543-550 | NNP | denotes | Medical |
T14495 | 551-557 | NNP | denotes | School |
T14496 | 558-564 | NNP | denotes | Animal |
T14497 | 565-569 | NNP | denotes | Care |
T14498 | 570-573 | CC | denotes | and |
T14499 | 574-577 | NNP | denotes | Use |
T14500 | 578-588 | NNS | denotes | Committees |
T14501 | 588-589 | . | denotes | . |
T14502 | 590-593 | IN | denotes | For |
T14503 | 594-605 | NN | denotes | stimulation |
T14504 | 605-606 | , | denotes | , |
T14505 | 607-615 | VBN | denotes | purified |
T14506 | 616-619 | NNP | denotes | CD8 |
T14507 | 619-620 | CD | denotes | + |
T14508 | 621-622 | NNP | denotes | T |
T14509 | 623-628 | NNS | denotes | cells |
T14510 | 629-633 | VBD | denotes | were |
T14511 | 634-642 | VBN | denotes | cultured |
T14512 | 643-645 | IN | denotes | at |
T14513 | 646-649 | CD | denotes | 106 |
T14514 | 650-658 | NN | denotes | cells/ml |
T14515 | 659-660 | -LRB- | denotes | ( |
T14516 | 660-662 | CD | denotes | 10 |
T14517 | 663-665 | NN | denotes | ml |
T14518 | 665-666 | -RRB- | denotes | ) |
T14519 | 667-669 | IN | denotes | in |
T14520 | 670-673 | CD | denotes | T25 |
T14521 | 674-680 | NNS | denotes | flasks |
T14522 | 681-687 | VBN | denotes | coated |
T14523 | 688-692 | IN | denotes | with |
T14524 | 693-694 | CD | denotes | 1 |
T14525 | 695-697 | NN | denotes | μg |
T14526 | 697-698 | NN | denotes | / |
T14527 | 698-700 | NN | denotes | ml |
T14528 | 701-705 | DT | denotes | each |
T14529 | 706-708 | IN | denotes | of |
T14530 | 709-717 | JJ | denotes | anti-CD3 |
T14531 | 718-719 | -LRB- | denotes | ( |
T14532 | 719-724 | JJ | denotes | clone |
T14533 | 725-729 | NNP | denotes | 2C11 |
T14534 | 729-730 | -RRB- | denotes | ) |
T14535 | 731-734 | CC | denotes | and |
T14536 | 735-744 | JJ | denotes | anti-CD28 |
T14537 | 745-746 | -LRB- | denotes | ( |
T14538 | 746-751 | JJ | denotes | clone |
T14539 | 752-757 | CD | denotes | 37.51 |
T14540 | 757-758 | -RRB- | denotes | ) |
T14541 | 759-761 | IN | denotes | by |
T14542 | 762-774 | NN | denotes | pretreatment |
T14543 | 775-779 | IN | denotes | with |
T14544 | 780-783 | CD | denotes | 300 |
T14545 | 784-786 | NN | denotes | μg |
T14546 | 786-787 | NN | denotes | / |
T14547 | 787-789 | NN | denotes | ml |
T14548 | 790-794 | NN | denotes | goat |
T14549 | 795-799 | NNS | denotes | anti |
T14550 | 800-807 | NN | denotes | hamster |
T14551 | 808-811 | NNP | denotes | IgG |
T14552 | 811-812 | . | denotes | . |
T14553 | 813-818 | IN | denotes | After |
T14554 | 819-821 | CD | denotes | 48 |
T14555 | 822-823 | NN | denotes | h |
T14556 | 823-824 | , | denotes | , |
T14557 | 825-830 | NNS | denotes | cells |
T14558 | 831-835 | VBD | denotes | were |
T14559 | 836-843 | VBN | denotes | removed |
T14560 | 844-848 | IN | denotes | from |
T14561 | 849-852 | DT | denotes | the |
T14562 | 853-856 | NNP | denotes | TCR |
T14563 | 857-868 | NN | denotes | stimulation |
T14564 | 869-872 | CC | denotes | and |
T14565 | 873-883 | VBD | denotes | recultured |
T14566 | 884-886 | IN | denotes | at |
T14567 | 887-888 | DT | denotes | a |
T14568 | 889-902 | NN | denotes | concentration |
T14569 | 903-905 | IN | denotes | of |
T14570 | 906-907 | CD | denotes | 5 |
T14571 | 908-909 | CD | denotes | × |
T14572 | 910-913 | CD | denotes | 105 |
T14573 | 914-922 | NN | denotes | cells/ml |
T14574 | 923-925 | IN | denotes | in |
T14575 | 926-931 | NNS | denotes | media |
T14576 | 932-944 | VBN | denotes | supplemented |
T14577 | 945-949 | IN | denotes | with |
T14578 | 950-953 | CD | denotes | 100 |
T14579 | 954-958 | NNP | denotes | U/ml |
T14580 | 959-965 | NNP | denotes | rhIL-2 |
T14581 | 965-966 | . | denotes | . |
T14582 | 967-972 | DT | denotes | Every |
T14583 | 973-975 | CD | denotes | 24 |
T14584 | 976-977 | NN | denotes | h |
T14585 | 977-978 | , | denotes | , |
T14586 | 979-985 | JJ | denotes | viable |
T14587 | 986-991 | NNS | denotes | cells |
T14588 | 992-996 | VBD | denotes | were |
T14589 | 997-1004 | VBN | denotes | counted |
T14590 | 1005-1008 | CC | denotes | and |
T14591 | 1009-1019 | VBN | denotes | readjusted |
T14592 | 1020-1022 | TO | denotes | to |
T14593 | 1023-1024 | CD | denotes | 5 |
T14594 | 1025-1026 | CD | denotes | × |
T14595 | 1027-1030 | CD | denotes | 105 |
T14596 | 1031-1039 | NN | denotes | cells/ml |
T14597 | 1040-1044 | IN | denotes | with |
T14598 | 1045-1050 | JJ | denotes | fresh |
T14599 | 1051-1056 | NNS | denotes | media |
T14600 | 1057-1067 | VBG | denotes | containing |
T14601 | 1068-1071 | DT | denotes | the |
T14602 | 1072-1085 | JJ | denotes | corresponding |
T14603 | 1086-1092 | NN | denotes | amount |
T14604 | 1093-1095 | IN | denotes | of |
T14605 | 1096-1102 | NN | denotes | rhIL-2 |
T14606 | 1102-1103 | . | denotes | . |
R11155 | T14382 | T14382 | ROOT | Isolation,Isolation |
R11156 | T14383 | T14382 | cc | and,Isolation |
R11157 | T14384 | T14382 | conj | culture,Isolation |
R11158 | T14385 | T14382 | prep | of,Isolation |
R11159 | T14386 | T14390 | amod | primary,cells |
R11160 | T14387 | T14390 | nmod | CD8,cells |
R11161 | T14388 | T14390 | nummod | +,cells |
R11162 | T14389 | T14390 | compound | T,cells |
R11163 | T14390 | T14385 | pobj | cells,of |
R11164 | T14391 | T14382 | punct | .,Isolation |
R11165 | T14392 | T14395 | nummod | CD8,cells |
R11166 | T14393 | T14395 | nummod | +,cells |
R11167 | T14394 | T14395 | compound | T,cells |
R11168 | T14395 | T14426 | nsubjpass | cells,purified |
R11169 | T14396 | T14395 | prep | from,cells |
R11170 | T14397 | T14396 | pobj | 4,from |
R11171 | T14398 | T14399 | compound | 8-wk-old,Tcra |
R11172 | T14399 | T14426 | nsubjpass | Tcra,purified |
R11173 | T14400 | T14406 | nmod | −,transgenic |
R11174 | T14401 | T14406 | nmod | /,transgenic |
R11175 | T14402 | T14406 | nmod | −,transgenic |
R11176 | T14403 | T14406 | nmod | ×,transgenic |
R11177 | T14404 | T14406 | nmod | P14,transgenic |
R11178 | T14405 | T14406 | nmod | TCR,transgenic |
R11179 | T14406 | T14412 | nmod | transgenic,WT |
R11180 | T14407 | T14408 | punct | (,Taconic |
R11181 | T14408 | T14406 | appos | Taconic,transgenic |
R11182 | T14409 | T14408 | punct | ),Taconic |
R11183 | T14410 | T14412 | punct | ",",WT |
R11184 | T14411 | T14412 | compound | C57BL/6J,WT |
R11185 | T14412 | T14399 | conj | WT,Tcra |
R11186 | T14413 | T14412 | punct | ",",WT |
R11187 | T14414 | T14412 | cc | or,WT |
R11188 | T14415 | T14418 | nummod | Tbx21,− |
R11189 | T14416 | T14418 | nummod | −,− |
R11190 | T14417 | T14418 | compound | /,− |
R11191 | T14418 | T14412 | conj | −,WT |
R11192 | T14419 | T14422 | punct | (,Laboratory |
R11193 | T14420 | T14422 | det | The,Laboratory |
R11194 | T14421 | T14422 | compound | Jackson,Laboratory |
R11195 | T14422 | T14418 | appos | Laboratory,− |
R11196 | T14423 | T14422 | punct | ),Laboratory |
R11197 | T14424 | T14418 | conj | mice,− |
R11198 | T14425 | T14426 | auxpass | were,purified |
R11199 | T14426 | T14426 | ROOT | purified,purified |
R11200 | T14427 | T14426 | punct | (,purified |
R11201 | T14428 | T14431 | compound | >,purity |
R11202 | T14429 | T14430 | nummod | 95,% |
R11203 | T14430 | T14431 | compound | %,purity |
R11204 | T14431 | T14426 | appos | purity,purified |
R11205 | T14432 | T14426 | punct | ),purified |
R11206 | T14433 | T14426 | agent | by,purified |
R11207 | T14434 | T14435 | amod | negative,selection |
R11208 | T14435 | T14433 | pobj | selection,by |
R11209 | T14436 | T14437 | punct | (,Invitrogen |
R11210 | T14437 | T14435 | appos | Invitrogen,selection |
R11211 | T14438 | T14437 | punct | ),Invitrogen |
R11212 | T14439 | T14435 | prep | from,selection |
R11213 | T14440 | T14441 | amod | pooled,spleen |
R11214 | T14441 | T14439 | pobj | spleen,from |
R11215 | T14442 | T14441 | cc | and,spleen |
R11216 | T14443 | T14445 | amod | lymph,cells |
R11217 | T14444 | T14445 | compound | node,cells |
R11218 | T14445 | T14441 | conj | cells,spleen |
R11219 | T14446 | T14426 | punct | .,purified |
R11220 | T14447 | T14450 | nummod | CD8,cells |
R11221 | T14448 | T14450 | nummod | +,cells |
R11222 | T14449 | T14450 | compound | T,cells |
R11223 | T14450 | T14462 | nsubjpass | cells,purified |
R11224 | T14451 | T14450 | prep | from,cells |
R11225 | T14452 | T14456 | amod | Runx3,mice |
R11226 | T14453 | T14456 | nmod | −,mice |
R11227 | T14454 | T14456 | nmod | /,mice |
R11228 | T14455 | T14456 | compound | −,mice |
R11229 | T14456 | T14451 | pobj | mice,from |
R11230 | T14457 | T14450 | prep | on,cells |
R11231 | T14458 | T14460 | det | the,background |
R11232 | T14459 | T14460 | compound | ICR,background |
R11233 | T14460 | T14457 | pobj | background,on |
R11234 | T14461 | T14462 | auxpass | were,purified |
R11235 | T14462 | T14462 | ROOT | purified,purified |
R11236 | T14463 | T14462 | agent | by,purified |
R11237 | T14464 | T14465 | amod | positive,selection |
R11238 | T14465 | T14463 | pobj | selection,by |
R11239 | T14466 | T14468 | punct | (,Biotec |
R11240 | T14467 | T14468 | compound | Miltenyi,Biotec |
R11241 | T14468 | T14465 | appos | Biotec,selection |
R11242 | T14469 | T14468 | punct | ),Biotec |
R11243 | T14470 | T14462 | punct | .,purified |
R11244 | T14471 | T14472 | det | All,mice |
R11245 | T14472 | T14474 | nsubjpass | mice,maintained |
R11246 | T14473 | T14474 | auxpass | were,maintained |
R11247 | T14474 | T14474 | ROOT | maintained,maintained |
R11248 | T14475 | T14474 | prep | in,maintained |
R11249 | T14476 | T14479 | amod | specific,facilities |
R11250 | T14477 | T14479 | amod | pathogen-free,facilities |
R11251 | T14478 | T14479 | compound | barrier,facilities |
R11252 | T14479 | T14475 | pobj | facilities,in |
R11253 | T14480 | T14474 | cc | and,maintained |
R11254 | T14481 | T14474 | conj | used,maintained |
R11255 | T14482 | T14481 | prep | according,used |
R11256 | T14483 | T14482 | prep | to,according |
R11257 | T14484 | T14483 | pobj | protocols,to |
R11258 | T14485 | T14484 | acl | approved,protocols |
R11259 | T14486 | T14485 | agent | by,approved |
R11260 | T14487 | T14490 | det | the,Institute |
R11261 | T14488 | T14490 | compound | Immune,Institute |
R11262 | T14489 | T14490 | compound | Disease,Institute |
R11263 | T14490 | T14486 | pobj | Institute,by |
R11264 | T14491 | T14490 | cc | and,Institute |
R11265 | T14492 | T14497 | det | the,Care |
R11266 | T14493 | T14495 | compound | Harvard,School |
R11267 | T14494 | T14495 | compound | Medical,School |
R11268 | T14495 | T14497 | compound | School,Care |
R11269 | T14496 | T14497 | compound | Animal,Care |
R11270 | T14497 | T14490 | conj | Care,Institute |
R11271 | T14498 | T14497 | cc | and,Care |
R11272 | T14499 | T14497 | conj | Use,Care |
R11273 | T14500 | T14497 | conj | Committees,Care |
R11274 | T14501 | T14474 | punct | .,maintained |
R11275 | T14502 | T14511 | prep | For,cultured |
R11276 | T14503 | T14502 | pobj | stimulation,For |
R11277 | T14504 | T14511 | punct | ",",cultured |
R11278 | T14505 | T14509 | amod | purified,cells |
R11279 | T14506 | T14509 | nmod | CD8,cells |
R11280 | T14507 | T14509 | nummod | +,cells |
R11281 | T14508 | T14509 | compound | T,cells |
R11282 | T14509 | T14511 | nsubjpass | cells,cultured |
R11283 | T14510 | T14511 | auxpass | were,cultured |
R11284 | T14511 | T14511 | ROOT | cultured,cultured |
R11285 | T14512 | T14511 | prep | at,cultured |
R11286 | T14513 | T14514 | nummod | 106,cells/ml |
R11287 | T14514 | T14512 | pobj | cells/ml,at |
R11288 | T14515 | T14517 | punct | (,ml |
R11289 | T14516 | T14517 | nummod | 10,ml |
R11290 | T14517 | T14514 | appos | ml,cells/ml |
R11291 | T14518 | T14517 | punct | ),ml |
R11292 | T14519 | T14511 | prep | in,cultured |
R11293 | T14520 | T14521 | nummod | T25,flasks |
R11294 | T14521 | T14519 | pobj | flasks,in |
R11295 | T14522 | T14511 | conj | coated,cultured |
R11296 | T14523 | T14522 | prep | with,coated |
R11297 | T14524 | T14525 | nummod | 1,μg |
R11298 | T14525 | T14527 | compound | μg,ml |
R11299 | T14526 | T14527 | compound | /,ml |
R11300 | T14527 | T14523 | pobj | ml,with |
R11301 | T14528 | T14527 | appos | each,ml |
R11302 | T14529 | T14528 | prep | of,each |
R11303 | T14530 | T14529 | pobj | anti-CD3,of |
R11304 | T14531 | T14533 | punct | (,2C11 |
R11305 | T14532 | T14533 | compound | clone,2C11 |
R11306 | T14533 | T14530 | appos | 2C11,anti-CD3 |
R11307 | T14534 | T14533 | punct | ),2C11 |
R11308 | T14535 | T14530 | cc | and,anti-CD3 |
R11309 | T14536 | T14530 | conj | anti-CD28,anti-CD3 |
R11310 | T14537 | T14538 | punct | (,clone |
R11311 | T14538 | T14536 | appos | clone,anti-CD28 |
R11312 | T14539 | T14538 | nummod | 37.51,clone |
R11313 | T14540 | T14538 | punct | ),clone |
R11314 | T14541 | T14538 | prep | by,clone |
R11315 | T14542 | T14541 | pobj | pretreatment,by |
R11316 | T14543 | T14538 | prep | with,clone |
R11317 | T14544 | T14545 | nummod | 300,μg |
R11318 | T14545 | T14549 | nmod | μg,anti |
R11319 | T14546 | T14549 | nmod | /,anti |
R11320 | T14547 | T14549 | compound | ml,anti |
R11321 | T14548 | T14549 | compound | goat,anti |
R11322 | T14549 | T14543 | pobj | anti,with |
R11323 | T14550 | T14551 | compound | hamster,IgG |
R11324 | T14551 | T14549 | appos | IgG,anti |
R11325 | T14552 | T14511 | punct | .,cultured |
R11326 | T14553 | T14559 | prep | After,removed |
R11327 | T14554 | T14555 | nummod | 48,h |
R11328 | T14555 | T14553 | pobj | h,After |
R11329 | T14556 | T14559 | punct | ",",removed |
R11330 | T14557 | T14559 | nsubjpass | cells,removed |
R11331 | T14558 | T14559 | auxpass | were,removed |
R11332 | T14559 | T14559 | ROOT | removed,removed |
R11333 | T14560 | T14559 | prep | from,removed |
R11334 | T14561 | T14563 | det | the,stimulation |
R11335 | T14562 | T14563 | compound | TCR,stimulation |
R11336 | T14563 | T14560 | pobj | stimulation,from |
R11337 | T14564 | T14559 | cc | and,removed |
R11338 | T14565 | T14559 | conj | recultured,removed |
R11339 | T14566 | T14565 | prep | at,recultured |
R11340 | T14567 | T14568 | det | a,concentration |
R11341 | T14568 | T14566 | pobj | concentration,at |
R11342 | T14569 | T14568 | prep | of,concentration |
R11343 | T14570 | T14573 | nummod | 5,cells/ml |
R11344 | T14571 | T14573 | nummod | ×,cells/ml |
R11345 | T14572 | T14573 | nummod | 105,cells/ml |
R11346 | T14573 | T14569 | pobj | cells/ml,of |
R11347 | T14574 | T14568 | prep | in,concentration |
R11348 | T14575 | T14574 | pobj | media,in |
R11349 | T14576 | T14565 | conj | supplemented,recultured |
R11350 | T14577 | T14576 | prep | with,supplemented |
R11351 | T14578 | T14580 | nummod | 100,rhIL-2 |
R11352 | T14579 | T14580 | compound | U/ml,rhIL-2 |
R11353 | T14580 | T14577 | pobj | rhIL-2,with |
R11354 | T14581 | T14559 | punct | .,removed |
R11355 | T14582 | T14584 | det | Every,h |
R11356 | T14583 | T14584 | nummod | 24,h |
R11357 | T14584 | T14589 | npadvmod | h,counted |
R11358 | T14585 | T14589 | punct | ",",counted |
R11359 | T14586 | T14587 | amod | viable,cells |
R11360 | T14587 | T14589 | nsubjpass | cells,counted |
R11361 | T14588 | T14589 | auxpass | were,counted |
R11362 | T14589 | T14589 | ROOT | counted,counted |
R11363 | T14590 | T14589 | cc | and,counted |
R11364 | T14591 | T14589 | conj | readjusted,counted |
R11365 | T14592 | T14591 | prep | to,readjusted |
R11366 | T14593 | T14596 | nummod | 5,cells/ml |
R11367 | T14594 | T14596 | nummod | ×,cells/ml |
R11368 | T14595 | T14596 | nummod | 105,cells/ml |
R11369 | T14596 | T14592 | pobj | cells/ml,to |
R11370 | T14597 | T14591 | prep | with,readjusted |
R11371 | T14598 | T14599 | amod | fresh,media |
R11372 | T14599 | T14597 | pobj | media,with |
R11373 | T14600 | T14597 | pcomp | containing,with |
R11374 | T14601 | T14603 | det | the,amount |
R11375 | T14602 | T14603 | amod | corresponding,amount |
R11376 | T14603 | T14600 | dobj | amount,containing |
R11377 | T14604 | T14603 | prep | of,amount |
R11378 | T14605 | T14604 | pobj | rhIL-2,of |
R11379 | T14606 | T14589 | punct | .,counted |
UBERON-AE
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14353 | 246-252 | http://purl.obolibrary.org/obo/UBERON_0002106 | denotes | spleen |
T14354 | 257-262 | http://purl.obolibrary.org/obo/UBERON_0002391 | denotes | lymph |
T14355 | 257-267 | http://purl.obolibrary.org/obo/UBERON_0000029 | denotes | lymph node |
GO-BP
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14374 | 90-93 | http://purl.obolibrary.org/obo/GO_0006283 | denotes | TCR |
T14375 | 853-856 | http://purl.obolibrary.org/obo/GO_0006283 | denotes | TCR |
GO-CC
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14827 | 40-45 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14828 | 54-59 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14829 | 268-273 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14830 | 282-287 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14831 | 623-628 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14832 | 650-655 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14833 | 825-830 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14834 | 914-919 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14835 | 986-991 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14836 | 1031-1036 | http://purl.obolibrary.org/obo/GO_0005623 | denotes | cells |
T14837 | 90-93 | http://purl.obolibrary.org/obo/GO_0042101 | denotes | TCR |
T14838 | 853-856 | http://purl.obolibrary.org/obo/GO_0042101 | denotes | TCR |
sentences
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14376 | 0-46 | Sentence | denotes | Isolation and culture of primary CD8+ T cells. |
T14377 | 47-274 | Sentence | denotes | CD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (>95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. |
T14378 | 275-383 | Sentence | denotes | CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). |
T14379 | 384-812 | Sentence | denotes | All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. |
T14380 | 813-966 | Sentence | denotes | After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. |
T14381 | 967-1103 | Sentence | denotes | Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2. |
T157 | 0-46 | Sentence | denotes | Isolation and culture of primary CD8+ T cells. |
T158 | 47-274 | Sentence | denotes | CD8+ T cells from 4–8-wk-old Tcra−/− × P14 TCR transgenic (Taconic), C57BL/6J WT, or Tbx21−/− (The Jackson Laboratory) mice were purified (>95% purity) by negative selection (Invitrogen) from pooled spleen and lymph node cells. |
T159 | 275-383 | Sentence | denotes | CD8+ T cells from Runx3−/− mice on the ICR background were purified by positive selection (Miltenyi Biotec). |
T160 | 384-589 | Sentence | denotes | All mice were maintained in specific pathogen-free barrier facilities and used according to protocols approved by the Immune Disease Institute and the Harvard Medical School Animal Care and Use Committees. |
T161 | 590-812 | Sentence | denotes | For stimulation, purified CD8+ T cells were cultured at 106 cells/ml (10 ml) in T25 flasks coated with 1 μg/ml each of anti-CD3 (clone 2C11) and anti-CD28 (clone 37.51) by pretreatment with 300 μg/ml goat anti–hamster IgG. |
T162 | 813-966 | Sentence | denotes | After 48 h, cells were removed from the TCR stimulation and recultured at a concentration of 5 × 105 cells/ml in media supplemented with 100 U/ml rhIL-2. |
T163 | 967-1103 | Sentence | denotes | Every 24 h, viable cells were counted and readjusted to 5 × 105 cells/ml with fresh media containing the corresponding amount of rhIL-2. |
events-check-again
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14884 | 47-50 | Protein | denotes | CD8 |
T14885 | 76-80 | Protein | denotes | Tcra |
T14886 | 132-137 | Protein | denotes | Tbx21 |
T14887 | 275-278 | Protein | denotes | CD8 |
T14888 | 293-298 | Protein | denotes | Runx3 |
T14889 | 616-619 | Protein | denotes | CD8 |
T14890 | 714-717 | Protein | denotes | CD3 |
T14891 | 740-744 | Protein | denotes | CD28 |
T14892 | 1096-1102 | Protein | denotes | rhIL-2 |
bionlp-st-ge-2016-reference-tees
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14893 | 33-37 | Protein | denotes | CD8+ |
T14894 | 47-50 | Protein | denotes | CD8 |
T14895 | 86-93 | Protein | denotes | P14 TCR |
T14896 | 132-137 | Protein | denotes | Tbx21 |
T14897 | 275-279 | Protein | denotes | CD8+ |
T14898 | 293-298 | Protein | denotes | Runx3 |
T14899 | 502-516 | Protein | denotes | Immune Disease |
T14900 | 616-620 | Protein | denotes | CD8+ |
T14901 | 709-717 | Protein | denotes | anti-CD3 |
T14902 | 719-729 | Protein | denotes | clone 2C11 |
T14903 | 740-744 | Protein | denotes | CD28 |
T14904 | 795-811 | Protein | denotes | anti–hamster IgG |
T14905 | 853-856 | Protein | denotes | TCR |
T14906 | 1096-1102 | Protein | denotes | rhIL-2 |
bionlp-st-ge-2016-reference
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14365 | 47-50 | Protein | denotes | CD8 |
T14366 | 76-80 | Protein | denotes | Tcra |
T14367 | 132-137 | Protein | denotes | Tbx21 |
T14368 | 275-278 | Protein | denotes | CD8 |
T14369 | 293-298 | Protein | denotes | Runx3 |
T14370 | 616-619 | Protein | denotes | CD8 |
T14371 | 714-717 | Protein | denotes | CD3 |
T14372 | 740-744 | Protein | denotes | CD28 |
T14373 | 1096-1102 | Protein | denotes | rhIL-2 |
bionlp-st-ge-2016-uniprot
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14806 | 33-36 | P01732 | denotes | CD8 |
T14807 | 33-36 | P10966 | denotes | CD8 |
T14808 | 47-50 | P10966 | denotes | CD8 |
T14809 | 47-50 | P01732 | denotes | CD8 |
T14810 | 132-137 | Q9UL17 | denotes | Tbx21 |
T14811 | 275-278 | P01732 | denotes | CD8 |
T14812 | 275-278 | P10966 | denotes | CD8 |
T14813 | 293-298 | Q13761 | denotes | Runx3 |
T14814 | 616-619 | P10966 | denotes | CD8 |
T14815 | 616-619 | P01732 | denotes | CD8 |
T14816 | 714-717 | P04234 | denotes | CD3 |
T14817 | 714-717 | P20963 | denotes | CD3 |
T14818 | 714-717 | P09693 | denotes | CD3 |
T14819 | 714-717 | P07766 | denotes | CD3 |
T14820 | 740-744 | P10747 | denotes | CD28 |
test2
Id | Subject | Object | Predicate | Lexical cue |
---|---|---|---|---|
T14356 | 47-50 | Protein | denotes | CD8 |
T14357 | 76-80 | Protein | denotes | Tcra |
T14358 | 132-137 | Protein | denotes | Tbx21 |
T14359 | 275-278 | Protein | denotes | CD8 |
T14360 | 293-298 | Protein | denotes | Runx3 |
T14361 | 616-619 | Protein | denotes | CD8 |
T14362 | 714-717 | Protein | denotes | CD3 |
T14363 | 740-744 | Protein | denotes | CD28 |
T14364 | 1096-1102 | Protein | denotes | rhIL-2 |