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pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

GO-BP

{"project":"GO-BP","denotations":[{"id":"T20963","span":{"begin":639,"end":654},"obj":"http://purl.obolibrary.org/obo/GO_2000144"},{"id":"T20964","span":{"begin":1151,"end":1166},"obj":"http://purl.obolibrary.org/obo/GO_0006351"},{"id":"T20965","span":{"begin":1230,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0006283"},{"id":"T20966","span":{"begin":1234,"end":1241},"obj":"http://purl.obolibrary.org/obo/GO_0023052"}],"text":"Figure 4. Runx3 controls Eomes, perforin, granzyme B, and IFN-γ expression in effector CTLs. Runx3+/+ or Runx3−/− CD8+ T cells were activated and transduced with retroviruses bearing an empty IRES-GFP cassette (GFP) or also encoding Eomes-VP16 (Eo-VP16) or Myc-Runx3 (Runx3). The frequency of transduced cells in the cultures was equivalent for all constructs (∼75–90% GFP+ cells; not depicted). (A) Protein expression in whole-cell extracts (day 6) was analyzed by immunoblotting. Overexpression of Eomes-VP16 cannot be detected with the Eomes antibody, as the C-terminal epitope is within the region that has been replaced with the VP16 transactivation domain. (B) Expression of granzyme B and IFN-γ after culture for 6 d and restimulation for 4 h with PMA and ionomycin was determined by intracellular staining. The percentage of positively stained cells is shown above the gate; the mean fluorescence intensity (MFI) of granzyme B staining for the total population is shown below the gate. The vertical gray lines indicate the MFI for WT GFP+ cells. Results are representative of at least two independent experiments. (C) Schematic diagram of the transcriptional network involving Runx3 and T-box factors. T-bet is induced by TCR signals and is essential for early IFN-γ expression. Runx3 is present in naive CD8+ T cells and represses Runx1 and induces Eomes, perforin, granzyme B, and IFN-γ expression. Eomes may participate in sustaining late IFN-γ expression, whereas Runx3 and Eomes (but not T-bet) may cooperate to activate perforin expression. The dotted line indicates the partial effect of T-bet deficiency on Gzmb mRNA but not granzyme B protein expression."}

GO-MF

{"project":"GO-MF","denotations":[{"id":"T21621","span":{"begin":545,"end":553},"obj":"http://purl.obolibrary.org/obo/GO_0003823"}],"text":"Figure 4. Runx3 controls Eomes, perforin, granzyme B, and IFN-γ expression in effector CTLs. Runx3+/+ or Runx3−/− CD8+ T cells were activated and transduced with retroviruses bearing an empty IRES-GFP cassette (GFP) or also encoding Eomes-VP16 (Eo-VP16) or Myc-Runx3 (Runx3). The frequency of transduced cells in the cultures was equivalent for all constructs (∼75–90% GFP+ cells; not depicted). (A) Protein expression in whole-cell extracts (day 6) was analyzed by immunoblotting. Overexpression of Eomes-VP16 cannot be detected with the Eomes antibody, as the C-terminal epitope is within the region that has been replaced with the VP16 transactivation domain. (B) Expression of granzyme B and IFN-γ after culture for 6 d and restimulation for 4 h with PMA and ionomycin was determined by intracellular staining. The percentage of positively stained cells is shown above the gate; the mean fluorescence intensity (MFI) of granzyme B staining for the total population is shown below the gate. The vertical gray lines indicate the MFI for WT GFP+ cells. Results are representative of at least two independent experiments. (C) Schematic diagram of the transcriptional network involving Runx3 and T-box factors. T-bet is induced by TCR signals and is essential for early IFN-γ expression. Runx3 is present in naive CD8+ T cells and represses Runx1 and induces Eomes, perforin, granzyme B, and IFN-γ expression. Eomes may participate in sustaining late IFN-γ expression, whereas Runx3 and Eomes (but not T-bet) may cooperate to activate perforin expression. The dotted line indicates the partial effect of T-bet deficiency on Gzmb mRNA but not granzyme B protein expression."}

GO-CC

{"project":"GO-CC","denotations":[{"id":"T21622","span":{"begin":121,"end":126},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T21623","span":{"begin":304,"end":309},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T21624","span":{"begin":374,"end":379},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T21625","span":{"begin":852,"end":857},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T21626","span":{"begin":1047,"end":1052},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T21627","span":{"begin":1320,"end":1325},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T21628","span":{"begin":545,"end":553},"obj":"http://purl.obolibrary.org/obo/GO_0019815"},{"id":"T21629","span":{"begin":545,"end":553},"obj":"http://purl.obolibrary.org/obo/GO_0042571"},{"id":"T21630","span":{"begin":791,"end":804},"obj":"http://purl.obolibrary.org/obo/GO_0005622"},{"id":"T21631","span":{"begin":1230,"end":1233},"obj":"http://purl.obolibrary.org/obo/GO_0042101"}],"text":"Figure 4. Runx3 controls Eomes, perforin, granzyme B, and IFN-γ expression in effector CTLs. Runx3+/+ or Runx3−/− CD8+ T cells were activated and transduced with retroviruses bearing an empty IRES-GFP cassette (GFP) or also encoding Eomes-VP16 (Eo-VP16) or Myc-Runx3 (Runx3). The frequency of transduced cells in the cultures was equivalent for all constructs (∼75–90% GFP+ cells; not depicted). (A) Protein expression in whole-cell extracts (day 6) was analyzed by immunoblotting. Overexpression of Eomes-VP16 cannot be detected with the Eomes antibody, as the C-terminal epitope is within the region that has been replaced with the VP16 transactivation domain. (B) Expression of granzyme B and IFN-γ after culture for 6 d and restimulation for 4 h with PMA and ionomycin was determined by intracellular staining. The percentage of positively stained cells is shown above the gate; the mean fluorescence intensity (MFI) of granzyme B staining for the total population is shown below the gate. The vertical gray lines indicate the MFI for WT GFP+ cells. Results are representative of at least two independent experiments. (C) Schematic diagram of the transcriptional network involving Runx3 and T-box factors. T-bet is induced by TCR signals and is essential for early IFN-γ expression. Runx3 is present in naive CD8+ T cells and represses Runx1 and induces Eomes, perforin, granzyme B, and IFN-γ expression. Eomes may participate in sustaining late IFN-γ expression, whereas Runx3 and Eomes (but not T-bet) may cooperate to activate perforin expression. The dotted line indicates the partial effect of T-bet deficiency on Gzmb mRNA but not granzyme B protein expression."}

sentences

{"project":"sentences","denotations":[{"id":"T20952","span":{"begin":10,"end":92},"obj":"Sentence"},{"id":"T20953","span":{"begin":93,"end":275},"obj":"Sentence"},{"id":"T20954","span":{"begin":276,"end":481},"obj":"Sentence"},{"id":"T20955","span":{"begin":482,"end":814},"obj":"Sentence"},{"id":"T20956","span":{"begin":815,"end":993},"obj":"Sentence"},{"id":"T20957","span":{"begin":994,"end":1053},"obj":"Sentence"},{"id":"T20958","span":{"begin":1054,"end":1209},"obj":"Sentence"},{"id":"T20959","span":{"begin":1210,"end":1286},"obj":"Sentence"},{"id":"T20960","span":{"begin":1287,"end":1408},"obj":"Sentence"},{"id":"T20961","span":{"begin":1409,"end":1554},"obj":"Sentence"},{"id":"T20962","span":{"begin":1555,"end":1671},"obj":"Sentence"},{"id":"T111","span":{"begin":0,"end":9},"obj":"Sentence"},{"id":"T112","span":{"begin":10,"end":92},"obj":"Sentence"},{"id":"T113","span":{"begin":93,"end":275},"obj":"Sentence"},{"id":"T114","span":{"begin":276,"end":481},"obj":"Sentence"},{"id":"T115","span":{"begin":482,"end":814},"obj":"Sentence"},{"id":"T116","span":{"begin":815,"end":993},"obj":"Sentence"},{"id":"T117","span":{"begin":994,"end":1053},"obj":"Sentence"},{"id":"T118","span":{"begin":1054,"end":1209},"obj":"Sentence"},{"id":"T119","span":{"begin":1210,"end":1286},"obj":"Sentence"},{"id":"T120","span":{"begin":1287,"end":1408},"obj":"Sentence"},{"id":"T121","span":{"begin":1409,"end":1554},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Figure 4. Runx3 controls Eomes, perforin, granzyme B, and IFN-γ expression in effector CTLs. Runx3+/+ or Runx3−/− CD8+ T cells were activated and transduced with retroviruses bearing an empty IRES-GFP cassette (GFP) or also encoding Eomes-VP16 (Eo-VP16) or Myc-Runx3 (Runx3). The frequency of transduced cells in the cultures was equivalent for all constructs (∼75–90% GFP+ cells; not depicted). (A) Protein expression in whole-cell extracts (day 6) was analyzed by immunoblotting. Overexpression of Eomes-VP16 cannot be detected with the Eomes antibody, as the C-terminal epitope is within the region that has been replaced with the VP16 transactivation domain. (B) Expression of granzyme B and IFN-γ after culture for 6 d and restimulation for 4 h with PMA and ionomycin was determined by intracellular staining. The percentage of positively stained cells is shown above the gate; the mean fluorescence intensity (MFI) of granzyme B staining for the total population is shown below the gate. The vertical gray lines indicate the MFI for WT GFP+ cells. Results are representative of at least two independent experiments. (C) Schematic diagram of the transcriptional network involving Runx3 and T-box factors. T-bet is induced by TCR signals and is essential for early IFN-γ expression. Runx3 is present in naive CD8+ T cells and represses Runx1 and induces Eomes, perforin, granzyme B, and IFN-γ expression. Eomes may participate in sustaining late IFN-γ expression, whereas Runx3 and Eomes (but not T-bet) may cooperate to activate perforin expression. The dotted line indicates the partial effect of T-bet deficiency on Gzmb mRNA but not granzyme B protein expression."}

events-check-again

bionlp-st-ge-2016-reference-tees

bionlp-st-ge-2016-reference

bionlp-st-ge-2016-uniprot

test2

PMC:2626671 / 19196-20867 JSONTXT

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PMC:2626671 / 19196-20867 JSON TXT