PMC:2586094 / 7031-7572 JSONTXT

{"target":"https://pubannotation.org/docs/sourcedb/PMC/sourceid/2586094","sourcedb":"PMC","sourceid":"2586094","source_url":"http://www.ncbi.nlm.nih.gov/pmc/2586094","text":"2.5 Immunoprecipitations and CK2 activity assays\nWhole cell extracts (150–300 µg) were prepared using buffers containing phosphatase and protease inhibitors and subjected to immunoprecipitation and in vitro kinase assays using the β-casein substrate as previously described [10]. For CK2 assays with Sp1, 0.5 µg of recombinant protein was used as a substrate instead of β-casein. For co-immunoprecipitation assays, the immunoprecipitated proteins were eluted using 0.1 M glycine (pH 2.5) and subjected to SDS-PAGE and western blot analysis.","divisions":[{"label":"Title","span":{"begin":5,"end":49}}],"tracks":[{"project":"2_test","denotations":[{"id":"18793716-12609974-20139052","span":{"begin":276,"end":278},"obj":"12609974"}],"attributes":[{"subj":"18793716-12609974-20139052","pred":"source","obj":"2_test"}]}],"config":{"attribute types":[{"pred":"source","value type":"selection","values":[{"id":"2_test","color":"#ec9593","default":true}]}]}}