PMC:2586094 / 7031-7572 JSONTXT

{"project":"2_test","denotations":[{"id":"18793716-12609974-20139052","span":{"begin":276,"end":278},"obj":"12609974"}],"text":"2.5 Immunoprecipitations and CK2 activity assays\nWhole cell extracts (150–300 µg) were prepared using buffers containing phosphatase and protease inhibitors and subjected to immunoprecipitation and in vitro kinase assays using the β-casein substrate as previously described [10]. For CK2 assays with Sp1, 0.5 µg of recombinant protein was used as a substrate instead of β-casein. For co-immunoprecipitation assays, the immunoprecipitated proteins were eluted using 0.1 M glycine (pH 2.5) and subjected to SDS-PAGE and western blot analysis."}