PMC:2518050 / 28382-29153 JSONTXT

{"project":"0_colil","denotations":[{"id":"18982121-11756499-359888","span":{"begin":765,"end":769},"obj":"11756499"}],"text":"We next determined the ratio of steady-state levels of exogenous, transgenic rat H1aV transcripts versus those encoding the endogenous Homer1 forms in TgH1aV.Fb mice. Mixed amplicons of 407 bp, generated by RT-PCR with generic Homer1 primers from either forebrain or hippocampal RNA, were sequenced to compare relative peak intensities in the 12 nucleotide positions in which the endogenous mouse sequence differs from the rat transcript. The H1aV transcripts were found to be in ∼five-fold excess over all endogenous Homer1 transcripts, both in forebrain and hippocampus (data not shown). Thus, the H1aV levels in the forebrain of TgH1aV.Fb mice were approximately those of Homer1a in wild-type mice after maximal electroconvulsive shock treatment (Bottai et al., 2002)."}

{"project":"TEST0","denotations":[{"id":"18982121-126-134-359888","span":{"begin":765,"end":769},"obj":"[\"11756499\"]"}],"text":"We next determined the ratio of steady-state levels of exogenous, transgenic rat H1aV transcripts versus those encoding the endogenous Homer1 forms in TgH1aV.Fb mice. Mixed amplicons of 407 bp, generated by RT-PCR with generic Homer1 primers from either forebrain or hippocampal RNA, were sequenced to compare relative peak intensities in the 12 nucleotide positions in which the endogenous mouse sequence differs from the rat transcript. The H1aV transcripts were found to be in ∼five-fold excess over all endogenous Homer1 transcripts, both in forebrain and hippocampus (data not shown). Thus, the H1aV levels in the forebrain of TgH1aV.Fb mice were approximately those of Homer1a in wild-type mice after maximal electroconvulsive shock treatment (Bottai et al., 2002)."}

{"project":"2_test","denotations":[{"id":"18982121-11756499-38286633","span":{"begin":765,"end":769},"obj":"11756499"}],"text":"We next determined the ratio of steady-state levels of exogenous, transgenic rat H1aV transcripts versus those encoding the endogenous Homer1 forms in TgH1aV.Fb mice. Mixed amplicons of 407 bp, generated by RT-PCR with generic Homer1 primers from either forebrain or hippocampal RNA, were sequenced to compare relative peak intensities in the 12 nucleotide positions in which the endogenous mouse sequence differs from the rat transcript. The H1aV transcripts were found to be in ∼five-fold excess over all endogenous Homer1 transcripts, both in forebrain and hippocampus (data not shown). Thus, the H1aV levels in the forebrain of TgH1aV.Fb mice were approximately those of Homer1a in wild-type mice after maximal electroconvulsive shock treatment (Bottai et al., 2002)."}