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pmc-enju-pas

bionlp-st-ge-2016-spacy-parsed

GO-CC

{"project":"GO-CC","denotations":[{"id":"T22946","span":{"begin":147,"end":152},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T22947","span":{"begin":221,"end":230},"obj":"http://purl.obolibrary.org/obo/GO_0000785"},{"id":"T22948","span":{"begin":336,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0000785"}],"text":"ChIP.\nChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature. The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin). The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h. Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction. The amount of DNA was determined by conventional PCR. The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers: 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′. The PCR products were visualized using an ethidium bromide gel."}

sentences

{"project":"sentences","denotations":[{"id":"T22565","span":{"begin":0,"end":5},"obj":"Sentence"},{"id":"T22566","span":{"begin":6,"end":216},"obj":"Sentence"},{"id":"T22567","span":{"begin":217,"end":331},"obj":"Sentence"},{"id":"T22568","span":{"begin":332,"end":490},"obj":"Sentence"},{"id":"T22569","span":{"begin":491,"end":725},"obj":"Sentence"},{"id":"T22570","span":{"begin":726,"end":779},"obj":"Sentence"},{"id":"T22571","span":{"begin":780,"end":887},"obj":"Sentence"},{"id":"T22572","span":{"begin":888,"end":949},"obj":"Sentence"},{"id":"T22573","span":{"begin":950,"end":1013},"obj":"Sentence"},{"id":"T334","span":{"begin":0,"end":5},"obj":"Sentence"},{"id":"T335","span":{"begin":6,"end":216},"obj":"Sentence"},{"id":"T336","span":{"begin":217,"end":331},"obj":"Sentence"},{"id":"T337","span":{"begin":332,"end":490},"obj":"Sentence"},{"id":"T338","span":{"begin":491,"end":580},"obj":"Sentence"},{"id":"T339","span":{"begin":581,"end":725},"obj":"Sentence"},{"id":"T340","span":{"begin":726,"end":779},"obj":"Sentence"},{"id":"T341","span":{"begin":780,"end":887},"obj":"Sentence"},{"id":"T342","span":{"begin":888,"end":949},"obj":"Sentence"},{"id":"T343","span":{"begin":950,"end":1013},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"ChIP.\nChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature. The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin). The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h. Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction. The amount of DNA was determined by conventional PCR. The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers: 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′. The PCR products were visualized using an ethidium bromide gel."}

events-check-again

{"project":"events-check-again","denotations":[{"id":"T22957","span":{"begin":427,"end":432},"obj":"Protein"},{"id":"T22958","span":{"begin":806,"end":811},"obj":"Protein"}],"text":"ChIP.\nChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature. The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin). The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h. Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction. The amount of DNA was determined by conventional PCR. The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers: 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′. The PCR products were visualized using an ethidium bromide gel."}

bionlp-st-ge-2016-reference-tees

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T22949","span":{"begin":388,"end":391},"obj":"Protein"},{"id":"T22950","span":{"begin":806,"end":820},"obj":"Protein"}],"text":"ChIP.\nChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature. The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin). The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h. Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction. The amount of DNA was determined by conventional PCR. The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers: 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′. The PCR products were visualized using an ethidium bromide gel."}

bionlp-st-ge-2016-reference

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T22563","span":{"begin":427,"end":432},"obj":"Protein"},{"id":"T22564","span":{"begin":806,"end":811},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"ChIP.\nChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature. The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin). The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h. Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction. The amount of DNA was determined by conventional PCR. The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers: 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′. The PCR products were visualized using an ethidium bromide gel."}

bionlp-st-ge-2016-uniprot

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T22743","span":{"begin":427,"end":432},"obj":"P23771"},{"id":"T22744","span":{"begin":806,"end":811},"obj":"Q9BZS1"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"ChIP.\nChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature. The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin). The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h. Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction. The amount of DNA was determined by conventional PCR. The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers: 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′. The PCR products were visualized using an ethidium bromide gel."}

test2

{"project":"test2","denotations":[{"id":"T22561","span":{"begin":427,"end":432},"obj":"Protein"},{"id":"T22562","span":{"begin":806,"end":811},"obj":"Protein"}],"text":"ChIP.\nChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature. The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin). The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h. Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction. The amount of DNA was determined by conventional PCR. The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers: 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′. The PCR products were visualized using an ethidium bromide gel."}

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PMC:2222968 / 44684-45697 JSON TXT