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PMC:2222968 / 44684-45697 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T22574 0-4 NN denotes ChIP
T22575 6-10 NN denotes ChIP
T22576 11-19 NN denotes analysis
T22577 20-23 VB denotes was
T22578 24-33 VB denotes performed
T22579 34-43 VB denotes according
T22580 44-46 TO denotes to
T22581 47-50 DT denotes the
T22582 51-63 NN denotes manufacturer
T22583 63-65 POS denotes 's
T22584 66-74 NN denotes protocol
T22585 75-76 -LRB- denotes (
T22586 76-83 NNP denotes Upstate
T22587 84-97 NNP denotes Biotechnology
T22588 97-98 -RRB- denotes )
T22589 99-103 IN denotes with
T22590 104-107 DT denotes the
T22591 108-117 VB denotes following
T22592 118-132 NN denotes modifications.
T22593 133-138 NN denotes iTreg
T22594 139-142 CC denotes and
T22595 143-146 NN denotes Th2
T22596 147-152 NN denotes cells
T22597 153-157 VB denotes were
T22598 158-163 VB denotes fixed
T22599 164-168 IN denotes with
T22600 169-170 CD denotes 1
T22601 170-171 NN denotes %
T22602 172-184 NN denotes formaldehyde
T22603 185-188 IN denotes for
T22604 189-191 CD denotes 10
T22605 192-195 NN denotes min
T22606 196-198 IN denotes at
T22607 199-203 NN denotes room
T22608 204-215 NN denotes temperature
T22609 217-220 DT denotes The
T22610 221-230 NN denotes chromatin
T22611 231-234 VB denotes was
T22612 235-242 VB denotes sheared
T22613 243-245 TO denotes to
T22614 246-254 CD denotes 200-1000
T22615 255-257 NN denotes bp
T22616 258-260 IN denotes of
T22617 261-267 NN denotes length
T22618 268-270 IN denotes by
T22619 271-281 NN denotes sonication
T22620 282-286 IN denotes with
T22621 287-291 CD denotes five
T22622 292-298 NN denotes pulses
T22623 299-301 IN denotes of
T22624 302-304 CD denotes 10
T22625 305-306 NN denotes s
T22626 307-309 IN denotes at
T22627 310-312 CD denotes 30
T22628 312-313 NN denotes %
T22629 314-319 NN denotes power
T22630 320-321 -LRB- denotes (
T22631 321-329 NN denotes Bandelin
T22632 329-330 -RRB- denotes )
T22633 332-335 DT denotes The
T22634 336-345 NN denotes chromatin
T22635 346-349 VB denotes was
T22636 350-361 JJ denotes pre-cleared
T22637 362-365 IN denotes for
T22638 366-367 CD denotes 2
T22639 368-369 NN denotes h
T22640 370-374 IN denotes with
T22641 375-381 JJ denotes normal
T22642 382-387 NN denotes mouse
T22643 388-391 NN denotes IgG
T22644 392-397 NN denotes beads
T22645 398-401 CC denotes and
T22646 402-406 RB denotes then
T22647 407-416 VB denotes incubated
T22648 417-421 IN denotes with
T22649 422-440 JJ denotes anti-GATA3-agarose
T22650 441-446 NN denotes beads
T22651 447-448 -LRB- denotes (
T22652 448-454 NN denotes HG3–31
T22653 454-455 -COLON- denotes ;
T22654 456-461 NNP denotes Santa
T22655 462-466 NNP denotes Cruz
T22656 467-480 NNP denotes Biotechnology
T22657 480-481 -RRB- denotes )
T22658 482-485 IN denotes for
T22659 486-487 CD denotes 2
T22660 488-489 NN denotes h
T22661 491-498 NN denotes Washing
T22662 499-502 CC denotes and
T22663 503-510 NN denotes elution
T22664 511-518 NN denotes buffers
T22665 519-523 VB denotes were
T22666 524-528 VB denotes used
T22667 529-538 VB denotes according
T22668 539-541 TO denotes to
T22669 542-545 DT denotes the
T22670 546-554 NN denotes protocol
T22671 555-557 IN denotes of
T22672 558-565 NNP denotes Upstate
T22673 566-580 NNP denotes Biotechnology.
T22674 581-591 NNP denotes Crosslinks
T22675 592-596 VB denotes were
T22676 597-605 VB denotes reversed
T22677 606-608 IN denotes by
T22678 609-619 NN denotes incubation
T22679 620-622 IN denotes at
T22680 623-625 CD denotes 65
T22681 626-628 NN denotes °C
T22682 629-632 IN denotes for
T22683 633-634 CD denotes 4
T22684 635-636 NN denotes h
T22685 637-639 IN denotes in
T22686 640-643 DT denotes the
T22687 644-652 NN denotes presence
T22688 653-655 IN denotes of
T22689 656-659 CD denotes 0.2
T22690 660-661 NN denotes M
T22691 662-666 NN denotes NaCl
T22692 666-667 -COMMA- denotes ,
T22693 668-671 CC denotes and
T22694 672-675 DT denotes the
T22695 676-679 NN denotes DNA
T22696 680-683 VB denotes was
T22697 684-692 VB denotes purified
T22698 693-695 IN denotes by
T22699 696-713 NN denotes phenol/chloroform
T22700 714-724 NN denotes extraction
T22701 726-729 DT denotes The
T22702 730-736 NN denotes amount
T22703 737-739 IN denotes of
T22704 740-743 NN denotes DNA
T22705 744-747 VB denotes was
T22706 748-758 VB denotes determined
T22707 759-761 IN denotes by
T22708 762-774 JJ denotes conventional
T22709 775-778 NN denotes PCR
T22710 780-783 DT denotes The
T22711 784-787 NN denotes PCR
T22712 788-797 VB denotes addressed
T22713 798-801 IN denotes for
T22714 802-805 DT denotes the
T22715 806-811 NN denotes FOXP3
T22716 812-820 NN denotes promoter
T22717 821-827 NN denotes region
T22718 828-832 CD denotes −246
T22719 833-835 TO denotes to
T22720 836-840 CD denotes −511
T22721 841-844 CC denotes and
T22722 845-848 VB denotes was
T22723 849-858 VB denotes performed
T22724 859-864 VB denotes using
T22725 865-868 DT denotes the
T22726 869-878 VB denotes following
T22727 879-886 NN denotes primers
T22728 886-887 -COLON- denotes :
T22729 888-906 JJ denotes 5′-gtgccctttacgagt
T22730 907-916 NN denotes catctg-3′
T22731 917-920 CC denotes and
T22732 921-948 NN denotes 5′-gtgccctttacgagtcatctg-3′
T22733 950-953 DT denotes The
T22734 954-957 NN denotes PCR
T22735 958-966 NN denotes products
T22736 967-971 VB denotes were
T22737 972-982 VB denotes visualized
T22738 983-988 VB denotes using
T22739 989-991 DT denotes an
T22740 992-1000 NN denotes ethidium
T22741 1001-1008 NN denotes bromide
T22742 1009-1012 NN denotes gel
R16797 T22576 T22575 arg1Of analysis,ChIP
R16798 T22576 T22577 arg1Of analysis,was
R16799 T22576 T22578 arg2Of analysis,performed
R16800 T22578 T22577 arg2Of performed,was
R16801 T22578 T22579 arg1Of performed,according
R16802 T22578 T22594 arg1Of performed,and
R16803 T22580 T22579 arg2Of to,according
R16804 T22582 T22581 arg1Of manufacturer,the
R16805 T22582 T22583 arg2Of manufacturer,'s
R16806 T22584 T22580 arg2Of protocol,to
R16807 T22584 T22583 arg1Of protocol,'s
R16808 T22584 T22585 arg1Of protocol,(
R16809 T22584 T22589 arg1Of protocol,with
R16810 T22587 T22585 arg2Of Biotechnology,(
R16811 T22587 T22586 arg1Of Biotechnology,Upstate
R16812 T22588 T22585 arg3Of ),(
R16813 T22593 T22589 arg2Of iTreg,with
R16814 T22593 T22590 arg1Of iTreg,the
R16815 T22593 T22591 arg1Of iTreg,following
R16816 T22593 T22592 arg1Of iTreg,modifications.
R16817 T22596 T22595 arg1Of cells,Th2
R16818 T22596 T22597 arg1Of cells,were
R16819 T22596 T22598 arg2Of cells,fixed
R16820 T22598 T22594 arg2Of fixed,and
R16821 T22598 T22597 arg2Of fixed,were
R16822 T22598 T22599 arg1Of fixed,with
R16823 T22598 T22603 arg1Of fixed,for
R16824 T22600 T22601 arg1Of 1,%
R16825 T22602 T22599 arg2Of formaldehyde,with
R16826 T22602 T22600 arg1Of formaldehyde,1
R16827 T22605 T22603 arg2Of min,for
R16828 T22605 T22604 arg1Of min,10
R16829 T22605 T22606 arg1Of min,at
R16830 T22608 T22606 arg2Of temperature,at
R16831 T22608 T22607 arg1Of temperature,room
R16832 T22610 T22609 arg1Of chromatin,The
R16833 T22610 T22611 arg1Of chromatin,was
R16834 T22610 T22612 arg2Of chromatin,sheared
R16835 T22612 T22611 arg2Of sheared,was
R16836 T22612 T22613 arg1Of sheared,to
R16837 T22612 T22620 arg1Of sheared,with
R16838 T22615 T22613 arg2Of bp,to
R16839 T22615 T22614 arg1Of bp,200-1000
R16840 T22615 T22616 arg1Of bp,of
R16841 T22617 T22616 arg2Of length,of
R16842 T22617 T22618 arg1Of length,by
R16843 T22619 T22618 arg2Of sonication,by
R16844 T22622 T22620 arg2Of pulses,with
R16845 T22622 T22621 arg1Of pulses,five
R16846 T22622 T22623 arg1Of pulses,of
R16847 T22625 T22623 arg2Of s,of
R16848 T22625 T22624 arg1Of s,10
R16849 T22625 T22626 arg1Of s,at
R16850 T22627 T22628 arg1Of 30,%
R16851 T22629 T22626 arg2Of power,at
R16852 T22629 T22627 arg1Of power,30
R16853 T22629 T22630 arg1Of power,(
R16854 T22631 T22630 arg2Of Bandelin,(
R16855 T22632 T22630 arg3Of ),(
R16856 T22634 T22633 arg1Of chromatin,The
R16857 T22634 T22635 arg1Of chromatin,was
R16858 T22634 T22636 arg1Of chromatin,pre-cleared
R16859 T22634 T22647 arg1Of chromatin,incubated
R16860 T22635 T22645 arg1Of was,and
R16861 T22636 T22635 arg2Of pre-cleared,was
R16862 T22636 T22637 arg1Of pre-cleared,for
R16863 T22639 T22637 arg2Of h,for
R16864 T22639 T22638 arg1Of h,2
R16865 T22639 T22640 arg1Of h,with
R16866 T22644 T22640 arg2Of beads,with
R16867 T22644 T22641 arg1Of beads,normal
R16868 T22644 T22642 arg1Of beads,mouse
R16869 T22644 T22643 arg1Of beads,IgG
R16870 T22647 T22645 arg2Of incubated,and
R16871 T22647 T22646 arg1Of incubated,then
R16872 T22647 T22648 arg1Of incubated,with
R16873 T22647 T22658 arg1Of incubated,for
R16874 T22650 T22648 arg2Of beads,with
R16875 T22650 T22649 arg1Of beads,anti-GATA3-agarose
R16876 T22650 T22651 arg1Of beads,(
R16877 T22652 T22651 arg2Of HG3–31,(
R16878 T22652 T22653 arg1Of HG3–31,;
R16879 T22656 T22653 arg2Of Biotechnology,;
R16880 T22656 T22654 arg1Of Biotechnology,Santa
R16881 T22656 T22655 arg1Of Biotechnology,Cruz
R16882 T22657 T22651 arg3Of ),(
R16883 T22660 T22658 arg2Of h,for
R16884 T22660 T22659 arg1Of h,2
R16885 T22661 T22675 arg1Of Washing,were
R16886 T22661 T22676 arg2Of Washing,reversed
R16887 T22664 T22662 arg1Of buffers,and
R16888 T22664 T22663 arg1Of buffers,elution
R16889 T22664 T22665 arg1Of buffers,were
R16890 T22664 T22666 arg2Of buffers,used
R16891 T22666 T22665 arg2Of used,were
R16892 T22666 T22667 arg1Of used,according
R16893 T22668 T22667 arg2Of to,according
R16894 T22670 T22668 arg2Of protocol,to
R16895 T22670 T22669 arg1Of protocol,the
R16896 T22670 T22671 arg1Of protocol,of
R16897 T22674 T22671 arg2Of Crosslinks,of
R16898 T22674 T22672 arg1Of Crosslinks,Upstate
R16899 T22674 T22673 arg1Of Crosslinks,Biotechnology.
R16900 T22676 T22665 modOf reversed,were
R16901 T22676 T22675 arg2Of reversed,were
R16902 T22676 T22679 arg1Of reversed,at
R16903 T22676 T22682 arg1Of reversed,for
R16904 T22676 T22685 arg1Of reversed,in
R16905 T22676 T22693 arg1Of reversed,and
R16906 T22678 T22676 arg1Of incubation,reversed
R16907 T22678 T22677 arg2Of incubation,by
R16908 T22681 T22679 arg2Of °C,at
R16909 T22681 T22680 arg1Of °C,65
R16910 T22684 T22682 arg2Of h,for
R16911 T22684 T22683 arg1Of h,4
R16912 T22687 T22685 arg2Of presence,in
R16913 T22687 T22686 arg1Of presence,the
R16914 T22687 T22688 arg1Of presence,of
R16915 T22689 T22690 arg1Of 0.2,M
R16916 T22691 T22688 arg2Of NaCl,of
R16917 T22691 T22689 arg1Of NaCl,0.2
R16918 T22693 T22692 arg1Of and,","
R16919 T22695 T22694 arg1Of DNA,the
R16920 T22695 T22696 arg1Of DNA,was
R16921 T22695 T22697 arg2Of DNA,purified
R16922 T22697 T22693 arg2Of purified,and
R16923 T22697 T22696 arg2Of purified,was
R16924 T22700 T22697 arg1Of extraction,purified
R16925 T22700 T22698 arg2Of extraction,by
R16926 T22700 T22699 arg1Of extraction,phenol/chloroform
R16927 T22702 T22701 arg1Of amount,The
R16928 T22702 T22703 arg1Of amount,of
R16929 T22702 T22705 arg1Of amount,was
R16930 T22702 T22706 arg2Of amount,determined
R16931 T22704 T22703 arg2Of DNA,of
R16932 T22706 T22705 arg2Of determined,was
R16933 T22709 T22706 arg1Of PCR,determined
R16934 T22709 T22707 arg2Of PCR,by
R16935 T22709 T22708 arg1Of PCR,conventional
R16936 T22711 T22710 arg1Of PCR,The
R16937 T22711 T22712 arg2Of PCR,addressed
R16938 T22711 T22722 arg1Of PCR,was
R16939 T22711 T22723 arg2Of PCR,performed
R16940 T22712 T22713 arg1Of addressed,for
R16941 T22717 T22713 arg2Of region,for
R16942 T22717 T22714 arg1Of region,the
R16943 T22717 T22715 arg1Of region,FOXP3
R16944 T22717 T22716 arg1Of region,promoter
R16945 T22717 T22718 arg1Of region,−246
R16946 T22717 T22719 arg1Of region,to
R16947 T22720 T22719 arg2Of −511,to
R16948 T22720 T22721 arg1Of −511,and
R16949 T22723 T22722 arg2Of performed,was
R16950 T22724 T22723 arg3Of using,performed
R16951 T22727 T22724 arg2Of primers,using
R16952 T22727 T22725 arg1Of primers,the
R16953 T22727 T22726 arg1Of primers,following
R16954 T22727 T22728 arg1Of primers,:
R16955 T22730 T22731 arg1Of catctg-3′,and
R16956 T22731 T22729 arg1Of and,5′-gtgccctttacgagt
R16957 T22732 T22731 arg2Of 5′-gtgccctttacgagtcatctg-3′,and
R16958 T22735 T22733 arg1Of products,The
R16959 T22735 T22734 arg1Of products,PCR
R16960 T22735 T22736 arg1Of products,were
R16961 T22735 T22737 arg2Of products,visualized
R16962 T22737 T22736 arg2Of visualized,were
R16963 T22738 T22737 arg3Of using,visualized
R16964 T22742 T22738 arg2Of gel,using
R16965 T22742 T22739 arg1Of gel,an
R16966 T22742 T22740 arg1Of gel,ethidium
R16967 T22742 T22741 arg1Of gel,bromide

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T22754 0-4 NN denotes ChIP
T22755 4-5 . denotes .
T22756 6-10 NN denotes ChIP
T22757 11-19 NN denotes analysis
T22758 20-23 VBD denotes was
T22759 24-33 VBN denotes performed
T22760 34-43 VBG denotes according
T22761 44-46 TO denotes to
T22762 47-50 DT denotes the
T22763 51-63 NN denotes manufacturer
T22764 63-65 POS denotes 's
T22765 66-74 NN denotes protocol
T22766 75-76 -LRB- denotes (
T22767 76-83 NNP denotes Upstate
T22768 84-97 NNP denotes Biotechnology
T22769 97-98 -RRB- denotes )
T22770 99-103 IN denotes with
T22771 104-107 DT denotes the
T22772 108-117 VBG denotes following
T22773 118-131 NNS denotes modifications
T22774 131-132 . denotes .
T22775 133-138 NNP denotes iTreg
T22776 139-142 CC denotes and
T22777 143-146 NNP denotes Th2
T22778 147-152 NNS denotes cells
T22779 153-157 VBD denotes were
T22780 158-163 VBN denotes fixed
T22781 164-168 IN denotes with
T22782 169-170 CD denotes 1
T22783 170-171 NN denotes %
T22784 172-184 NN denotes formaldehyde
T22785 185-188 IN denotes for
T22786 189-191 CD denotes 10
T22787 192-195 NN denotes min
T22788 196-198 IN denotes at
T22789 199-203 NN denotes room
T22790 204-215 NN denotes temperature
T22791 215-216 . denotes .
T22792 217-220 DT denotes The
T22793 221-230 NN denotes chromatin
T22794 231-234 VBD denotes was
T22795 235-242 VBN denotes sheared
T22796 243-245 TO denotes to
T22797 246-254 CD denotes 200-1000
T22798 255-257 NN denotes bp
T22799 258-260 IN denotes of
T22800 261-267 NN denotes length
T22801 268-270 IN denotes by
T22802 271-281 NN denotes sonication
T22803 282-286 IN denotes with
T22804 287-291 CD denotes five
T22805 292-298 NNS denotes pulses
T22806 299-301 IN denotes of
T22807 302-304 CD denotes 10
T22808 305-306 PRP denotes s
T22809 307-309 IN denotes at
T22810 310-312 CD denotes 30
T22811 312-313 NN denotes %
T22812 314-319 NN denotes power
T22813 320-321 -LRB- denotes (
T22814 321-329 NNP denotes Bandelin
T22815 329-330 -RRB- denotes )
T22816 330-331 . denotes .
T22817 332-335 DT denotes The
T22818 336-345 NN denotes chromatin
T22819 346-349 VBD denotes was
T22820 350-361 JJ denotes pre-cleared
T22821 362-365 IN denotes for
T22822 366-367 CD denotes 2
T22823 368-369 NN denotes h
T22824 370-374 IN denotes with
T22825 375-381 JJ denotes normal
T22826 382-387 NN denotes mouse
T22827 388-391 NNP denotes IgG
T22828 392-397 NNS denotes beads
T22829 398-401 CC denotes and
T22830 402-406 RB denotes then
T22831 407-416 VBN denotes incubated
T22832 417-421 IN denotes with
T22833 422-440 JJ denotes anti-GATA3-agarose
T22834 441-446 NNS denotes beads
T22835 447-448 -LRB- denotes (
T22836 448-451 CD denotes HG3
T22837 452-454 CD denotes 31
T22838 454-455 : denotes ;
T22839 456-461 NNP denotes Santa
T22840 462-466 NNP denotes Cruz
T22841 467-480 NNP denotes Biotechnology
T22842 480-481 -RRB- denotes )
T22843 482-485 IN denotes for
T22844 486-487 CD denotes 2
T22845 488-490 NN denotes h.
T22846 491-498 VBG denotes Washing
T22847 499-502 CC denotes and
T22848 503-510 NN denotes elution
T22849 511-518 NNS denotes buffers
T22850 519-523 VBD denotes were
T22851 524-528 VBN denotes used
T22852 529-538 VBG denotes according
T22853 539-541 TO denotes to
T22854 542-545 DT denotes the
T22855 546-554 NN denotes protocol
T22856 555-557 IN denotes of
T22857 558-565 NNP denotes Upstate
T22858 566-579 NNP denotes Biotechnology
T22859 579-580 . denotes .
T22860 581-591 NNS denotes Crosslinks
T22861 592-596 VBD denotes were
T22862 597-605 VBN denotes reversed
T22863 606-608 IN denotes by
T22864 609-619 NN denotes incubation
T22865 620-622 IN denotes at
T22866 623-625 CD denotes 65
T22867 626-627 CD denotes °
T22868 627-628 NNP denotes C
T22869 629-632 IN denotes for
T22870 633-634 CD denotes 4
T22871 635-636 NN denotes h
T22872 637-639 IN denotes in
T22873 640-643 DT denotes the
T22874 644-652 NN denotes presence
T22875 653-655 IN denotes of
T22876 656-659 CD denotes 0.2
T22877 660-661 NNP denotes M
T22878 662-666 NNP denotes NaCl
T22879 666-667 , denotes ,
T22880 668-671 CC denotes and
T22881 672-675 DT denotes the
T22882 676-679 NN denotes DNA
T22883 680-683 VBD denotes was
T22884 684-692 VBN denotes purified
T22885 693-695 IN denotes by
T22886 696-713 NN denotes phenol/chloroform
T22887 714-724 NN denotes extraction
T22888 724-725 . denotes .
T22889 726-729 DT denotes The
T22890 730-736 NN denotes amount
T22891 737-739 IN denotes of
T22892 740-743 NNP denotes DNA
T22893 744-747 VBD denotes was
T22894 748-758 VBN denotes determined
T22895 759-761 IN denotes by
T22896 762-774 JJ denotes conventional
T22897 775-778 NNP denotes PCR
T22898 778-779 . denotes .
T22899 780-783 DT denotes The
T22900 784-787 NNP denotes PCR
T22901 788-797 VBD denotes addressed
T22902 798-801 IN denotes for
T22903 802-805 DT denotes the
T22904 806-811 CD denotes FOXP3
T22905 812-820 NN denotes promoter
T22906 821-827 NN denotes region
T22907 828-829 NN denotes −
T22908 829-832 CD denotes 246
T22909 833-835 TO denotes to
T22910 836-837 CD denotes −
T22911 837-840 CD denotes 511
T22912 841-844 CC denotes and
T22913 845-848 VBD denotes was
T22914 849-858 VBN denotes performed
T22915 859-864 VBG denotes using
T22916 865-868 DT denotes the
T22917 869-878 VBG denotes following
T22918 879-886 NNS denotes primers
T22919 886-887 : denotes :
T22920 888-889 CD denotes 5
T22921 889-890 SYM denotes ′
T22922 890-891 : denotes -
T22923 891-906 NN denotes gtgccctttacgagt
T22924 907-915 JJ denotes catctg-3
T22925 915-916 NN denotes ′
T22926 917-920 CC denotes and
T22927 921-922 CD denotes 5
T22928 922-923 SYM denotes ′
T22929 923-924 : denotes -
T22930 924-947 JJ denotes gtgccctttacgagtcatctg-3
T22931 947-948 NN denotes ′
T22932 948-949 . denotes .
T22933 950-953 DT denotes The
T22934 954-957 NNP denotes PCR
T22935 958-966 NNS denotes products
T22936 967-971 VBD denotes were
T22937 972-982 VBN denotes visualized
T22938 983-988 VBG denotes using
T22939 989-991 DT denotes an
T22940 992-1000 NN denotes ethidium
T22941 1001-1008 NN denotes bromide
T22942 1009-1012 NN denotes gel
T22943 1012-1013 . denotes .
R16968 T22754 T22754 ROOT ChIP,ChIP
R16969 T22755 T22754 punct .,ChIP
R16970 T22756 T22757 compound ChIP,analysis
R16971 T22757 T22759 nsubjpass analysis,performed
R16972 T22758 T22759 auxpass was,performed
R16973 T22759 T22759 ROOT performed,performed
R16974 T22760 T22759 prep according,performed
R16975 T22761 T22760 prep to,according
R16976 T22762 T22763 det the,manufacturer
R16977 T22763 T22765 poss manufacturer,protocol
R16978 T22764 T22763 case 's,manufacturer
R16979 T22765 T22761 pobj protocol,to
R16980 T22766 T22768 punct (,Biotechnology
R16981 T22767 T22768 compound Upstate,Biotechnology
R16982 T22768 T22765 appos Biotechnology,protocol
R16983 T22769 T22759 punct ),performed
R16984 T22770 T22759 prep with,performed
R16985 T22771 T22773 det the,modifications
R16986 T22772 T22773 amod following,modifications
R16987 T22773 T22770 pobj modifications,with
R16988 T22774 T22759 punct .,performed
R16989 T22775 T22778 nmod iTreg,cells
R16990 T22776 T22775 cc and,iTreg
R16991 T22777 T22775 conj Th2,iTreg
R16992 T22778 T22780 nsubjpass cells,fixed
R16993 T22779 T22780 auxpass were,fixed
R16994 T22780 T22780 ROOT fixed,fixed
R16995 T22781 T22780 prep with,fixed
R16996 T22782 T22783 nummod 1,%
R16997 T22783 T22784 compound %,formaldehyde
R16998 T22784 T22781 pobj formaldehyde,with
R16999 T22785 T22780 prep for,fixed
R17000 T22786 T22787 nummod 10,min
R17001 T22787 T22785 pobj min,for
R17002 T22788 T22787 prep at,min
R17003 T22789 T22790 compound room,temperature
R17004 T22790 T22788 pobj temperature,at
R17005 T22791 T22780 punct .,fixed
R17006 T22792 T22793 det The,chromatin
R17007 T22793 T22795 nsubjpass chromatin,sheared
R17008 T22794 T22795 auxpass was,sheared
R17009 T22795 T22795 ROOT sheared,sheared
R17010 T22796 T22795 prep to,sheared
R17011 T22797 T22798 nummod 200-1000,bp
R17012 T22798 T22796 pobj bp,to
R17013 T22799 T22798 prep of,bp
R17014 T22800 T22799 pobj length,of
R17015 T22801 T22795 agent by,sheared
R17016 T22802 T22801 pobj sonication,by
R17017 T22803 T22795 prep with,sheared
R17018 T22804 T22805 nummod five,pulses
R17019 T22805 T22803 pobj pulses,with
R17020 T22806 T22805 prep of,pulses
R17021 T22807 T22806 pobj 10,of
R17022 T22808 T22807 npadvmod s,10
R17023 T22809 T22805 prep at,pulses
R17024 T22810 T22811 nummod 30,%
R17025 T22811 T22812 compound %,power
R17026 T22812 T22809 pobj power,at
R17027 T22813 T22814 punct (,Bandelin
R17028 T22814 T22812 appos Bandelin,power
R17029 T22815 T22814 punct ),Bandelin
R17030 T22816 T22795 punct .,sheared
R17031 T22817 T22818 det The,chromatin
R17032 T22818 T22819 nsubj chromatin,was
R17033 T22819 T22851 ccomp was,used
R17034 T22820 T22819 acomp pre-cleared,was
R17035 T22821 T22820 prep for,pre-cleared
R17036 T22822 T22823 nummod 2,h
R17037 T22823 T22821 pobj h,for
R17038 T22824 T22823 prep with,h
R17039 T22825 T22826 amod normal,mouse
R17040 T22826 T22828 compound mouse,beads
R17041 T22827 T22828 compound IgG,beads
R17042 T22828 T22824 pobj beads,with
R17043 T22829 T22819 cc and,was
R17044 T22830 T22831 advmod then,incubated
R17045 T22831 T22819 conj incubated,was
R17046 T22832 T22831 prep with,incubated
R17047 T22833 T22834 amod anti-GATA3-agarose,beads
R17048 T22834 T22832 pobj beads,with
R17049 T22835 T22834 punct (,beads
R17050 T22836 T22834 appos HG3,beads
R17051 T22837 T22834 appos 31,beads
R17052 T22838 T22851 punct ;,used
R17053 T22839 T22840 compound Santa,Cruz
R17054 T22840 T22841 compound Cruz,Biotechnology
R17055 T22841 T22851 nsubjpass Biotechnology,used
R17056 T22842 T22841 punct ),Biotechnology
R17057 T22843 T22841 prep for,Biotechnology
R17058 T22844 T22845 nummod 2,h.
R17059 T22845 T22843 pobj h.,for
R17060 T22846 T22845 acl Washing,h.
R17061 T22847 T22846 cc and,Washing
R17062 T22848 T22849 compound elution,buffers
R17063 T22849 T22851 nsubjpass buffers,used
R17064 T22850 T22851 auxpass were,used
R17065 T22851 T22851 ROOT used,used
R17066 T22852 T22851 prep according,used
R17067 T22853 T22852 prep to,according
R17068 T22854 T22855 det the,protocol
R17069 T22855 T22853 pobj protocol,to
R17070 T22856 T22855 prep of,protocol
R17071 T22857 T22858 compound Upstate,Biotechnology
R17072 T22858 T22856 pobj Biotechnology,of
R17073 T22859 T22851 punct .,used
R17074 T22860 T22862 nsubjpass Crosslinks,reversed
R17075 T22861 T22862 auxpass were,reversed
R17076 T22862 T22862 ROOT reversed,reversed
R17077 T22863 T22862 agent by,reversed
R17078 T22864 T22863 pobj incubation,by
R17079 T22865 T22862 prep at,reversed
R17080 T22866 T22868 nummod 65,C
R17081 T22867 T22868 nummod °,C
R17082 T22868 T22865 pobj C,at
R17083 T22869 T22862 prep for,reversed
R17084 T22870 T22871 nummod 4,h
R17085 T22871 T22869 pobj h,for
R17086 T22872 T22862 prep in,reversed
R17087 T22873 T22874 det the,presence
R17088 T22874 T22872 pobj presence,in
R17089 T22875 T22874 prep of,presence
R17090 T22876 T22878 nummod 0.2,NaCl
R17091 T22877 T22878 compound M,NaCl
R17092 T22878 T22875 pobj NaCl,of
R17093 T22879 T22862 punct ",",reversed
R17094 T22880 T22862 cc and,reversed
R17095 T22881 T22882 det the,DNA
R17096 T22882 T22884 nsubjpass DNA,purified
R17097 T22883 T22884 auxpass was,purified
R17098 T22884 T22862 conj purified,reversed
R17099 T22885 T22884 agent by,purified
R17100 T22886 T22887 compound phenol/chloroform,extraction
R17101 T22887 T22885 pobj extraction,by
R17102 T22888 T22862 punct .,reversed
R17103 T22889 T22890 det The,amount
R17104 T22890 T22894 nsubjpass amount,determined
R17105 T22891 T22890 prep of,amount
R17106 T22892 T22891 pobj DNA,of
R17107 T22893 T22894 auxpass was,determined
R17108 T22894 T22894 ROOT determined,determined
R17109 T22895 T22894 agent by,determined
R17110 T22896 T22897 amod conventional,PCR
R17111 T22897 T22895 pobj PCR,by
R17112 T22898 T22894 punct .,determined
R17113 T22899 T22900 det The,PCR
R17114 T22900 T22901 nsubj PCR,addressed
R17115 T22901 T22901 ROOT addressed,addressed
R17116 T22902 T22901 prep for,addressed
R17117 T22903 T22906 det the,region
R17118 T22904 T22906 amod FOXP3,region
R17119 T22905 T22906 compound promoter,region
R17120 T22906 T22902 pobj region,for
R17121 T22907 T22901 dobj −,addressed
R17122 T22908 T22907 nummod 246,−
R17123 T22909 T22908 prep to,246
R17124 T22910 T22909 pobj −,to
R17125 T22911 T22910 appos 511,−
R17126 T22912 T22901 cc and,addressed
R17127 T22913 T22914 auxpass was,performed
R17128 T22914 T22901 conj performed,addressed
R17129 T22915 T22914 xcomp using,performed
R17130 T22916 T22918 det the,primers
R17131 T22917 T22918 amod following,primers
R17132 T22918 T22915 dobj primers,using
R17133 T22919 T22918 punct :,primers
R17134 T22920 T22925 nummod 5,′
R17135 T22921 T22923 punct ′,gtgccctttacgagt
R17136 T22922 T22923 punct -,gtgccctttacgagt
R17137 T22923 T22925 nmod gtgccctttacgagt,′
R17138 T22924 T22925 amod catctg-3,′
R17139 T22925 T22918 appos ′,primers
R17140 T22926 T22925 cc and,′
R17141 T22927 T22930 nummod 5,gtgccctttacgagtcatctg-3
R17142 T22928 T22930 punct ′,gtgccctttacgagtcatctg-3
R17143 T22929 T22930 punct -,gtgccctttacgagtcatctg-3
R17144 T22930 T22931 amod gtgccctttacgagtcatctg-3,′
R17145 T22931 T22925 conj ′,′
R17146 T22932 T22901 punct .,addressed
R17147 T22933 T22935 det The,products
R17148 T22934 T22935 compound PCR,products
R17149 T22935 T22937 nsubjpass products,visualized
R17150 T22936 T22937 auxpass were,visualized
R17151 T22937 T22937 ROOT visualized,visualized
R17152 T22938 T22937 advcl using,visualized
R17153 T22939 T22942 det an,gel
R17154 T22940 T22942 amod ethidium,gel
R17155 T22941 T22942 compound bromide,gel
R17156 T22942 T22938 dobj gel,using
R17157 T22943 T22937 punct .,visualized

GO-CC

Id Subject Object Predicate Lexical cue
T22946 147-152 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22947 221-230 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin
T22948 336-345 http://purl.obolibrary.org/obo/GO_0000785 denotes chromatin

sentences

Id Subject Object Predicate Lexical cue
T22565 0-5 Sentence denotes ChIP.
T22566 6-216 Sentence denotes ChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature.
T22567 217-331 Sentence denotes The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin).
T22568 332-490 Sentence denotes The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h.
T22569 491-725 Sentence denotes Washing and elution buffers were used according to the protocol of Upstate Biotechnology. Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction.
T22570 726-779 Sentence denotes The amount of DNA was determined by conventional PCR.
T22571 780-887 Sentence denotes The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers:
T22572 888-949 Sentence denotes 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′.
T22573 950-1013 Sentence denotes The PCR products were visualized using an ethidium bromide gel.
T334 0-5 Sentence denotes ChIP.
T335 6-216 Sentence denotes ChIP analysis was performed according to the manufacturer's protocol (Upstate Biotechnology) with the following modifications. iTreg and Th2 cells were fixed with 1% formaldehyde for 10 min at room temperature.
T336 217-331 Sentence denotes The chromatin was sheared to 200-1000 bp of length by sonication with five pulses of 10 s at 30% power (Bandelin).
T337 332-490 Sentence denotes The chromatin was pre-cleared for 2 h with normal mouse IgG beads and then incubated with anti-GATA3-agarose beads (HG3–31; Santa Cruz Biotechnology) for 2 h.
T338 491-580 Sentence denotes Washing and elution buffers were used according to the protocol of Upstate Biotechnology.
T339 581-725 Sentence denotes Crosslinks were reversed by incubation at 65 °C for 4 h in the presence of 0.2 M NaCl, and the DNA was purified by phenol/chloroform extraction.
T340 726-779 Sentence denotes The amount of DNA was determined by conventional PCR.
T341 780-887 Sentence denotes The PCR addressed for the FOXP3 promoter region −246 to −511 and was performed using the following primers:
T342 888-949 Sentence denotes 5′-gtgccctttacgagt catctg-3′ and 5′-gtgccctttacgagtcatctg-3′.
T343 950-1013 Sentence denotes The PCR products were visualized using an ethidium bromide gel.

events-check-again

Id Subject Object Predicate Lexical cue
T22957 427-432 Protein denotes GATA3
T22958 806-811 Protein denotes FOXP3

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T22949 388-391 Protein denotes IgG
T22950 806-820 Protein denotes FOXP3 promoter

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T22563 427-432 Protein denotes GATA3
T22564 806-811 Protein denotes FOXP3

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T22743 427-432 P23771 denotes GATA3
T22744 806-811 Q9BZS1 denotes FOXP3

test2

Id Subject Object Predicate Lexical cue
T22561 427-432 Protein denotes GATA3
T22562 806-811 Protein denotes FOXP3