PMC:2222968 / 43245-44151
Annnotations
pmc-enju-pas
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blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
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t","subj":"T22128","obj":"T22122"},{"id":"R16540","pred":"appos","subj":"T22129","obj":"T22113"},{"id":"R16541","pred":"punct","subj":"T22130","obj":"T22129"},{"id":"R16542","pred":"appos","subj":"T22131","obj":"T22129"},{"id":"R16543","pred":"punct","subj":"T22132","obj":"T22129"},{"id":"R16544","pred":"punct","subj":"T22133","obj":"T22129"},{"id":"R16545","pred":"appos","subj":"T22134","obj":"T22129"},{"id":"R16546","pred":"punct","subj":"T22135","obj":"T22136"},{"id":"R16547","pred":"appos","subj":"T22136","obj":"T22134"},{"id":"R16548","pred":"punct","subj":"T22137","obj":"T22136"},{"id":"R16549","pred":"punct","subj":"T22138","obj":"T22134"},{"id":"R16550","pred":"cc","subj":"T22139","obj":"T22134"},{"id":"R16551","pred":"conj","subj":"T22140","obj":"T22134"},{"id":"R16552","pred":"punct","subj":"T22141","obj":"T22144"},{"id":"R16553","pred":"compound","subj":"T22142","obj":"T22144"},{"id":"R16554","pred":"compound","subj":"T22143","obj":"T22144"},{"id":"R16555","pred":"parataxis","subj":"T22144","obj":"T22140"},{"id":"R16556","pred":"punct","subj":"T22145","obj":"T22144"},{"id":"R16557","pred":"punct","subj":"T22146","obj":"T22110"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
GO-MF
{"project":"GO-MF","denotations":[{"id":"T22157","span":{"begin":294,"end":301},"obj":"http://purl.obolibrary.org/obo/GO_0005488"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
GO-CC
{"project":"GO-CC","denotations":[{"id":"T22158","span":{"begin":77,"end":82},"obj":"http://purl.obolibrary.org/obo/GO_0005623"},{"id":"T22159","span":{"begin":249,"end":257},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T22160","span":{"begin":332,"end":341},"obj":"http://purl.obolibrary.org/obo/GO_0016020"},{"id":"T22161","span":{"begin":660,"end":669},"obj":"http://purl.obolibrary.org/obo/GO_0016020"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
sentences
{"project":"sentences","denotations":[{"id":"T21784","span":{"begin":0,"end":17},"obj":"Sentence"},{"id":"T21785","span":{"begin":18,"end":203},"obj":"Sentence"},{"id":"T21786","span":{"begin":204,"end":282},"obj":"Sentence"},{"id":"T21787","span":{"begin":283,"end":586},"obj":"Sentence"},{"id":"T21788","span":{"begin":587,"end":655},"obj":"Sentence"},{"id":"T21789","span":{"begin":656,"end":906},"obj":"Sentence"},{"id":"T323","span":{"begin":0,"end":17},"obj":"Sentence"},{"id":"T324","span":{"begin":18,"end":203},"obj":"Sentence"},{"id":"T325","span":{"begin":204,"end":282},"obj":"Sentence"},{"id":"T326","span":{"begin":283,"end":457},"obj":"Sentence"},{"id":"T327","span":{"begin":458,"end":586},"obj":"Sentence"},{"id":"T328","span":{"begin":587,"end":655},"obj":"Sentence"},{"id":"T329","span":{"begin":656,"end":906},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
events-check-again
{"project":"events-check-again","denotations":[{"id":"T22201","span":{"begin":22,"end":27},"obj":"Protein"},{"id":"T22202","span":{"begin":67,"end":70},"obj":"Protein"},{"id":"T22203","span":{"begin":71,"end":75},"obj":"Protein"},{"id":"T22204","span":{"begin":406,"end":411},"obj":"Protein"},{"id":"T22205","span":{"begin":696,"end":701},"obj":"Protein"},{"id":"T22206","span":{"begin":743,"end":748},"obj":"Protein"},{"id":"T22207","span":{"begin":788,"end":793},"obj":"Protein"},{"id":"T22208","span":{"begin":822,"end":827},"obj":"Protein"},{"id":"T22209","span":{"begin":850,"end":855},"obj":"Protein"},{"id":"T22210","span":{"begin":872,"end":877},"obj":"Protein"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
bionlp-st-ge-2016-reference-tees
{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T22162","span":{"begin":22,"end":27},"obj":"Protein"},{"id":"T22163","span":{"begin":67,"end":70},"obj":"Protein"},{"id":"T22164","span":{"begin":71,"end":75},"obj":"Protein"},{"id":"T22165","span":{"begin":396,"end":411},"obj":"Protein"},{"id":"T22166","span":{"begin":514,"end":517},"obj":"Protein"},{"id":"T22167","span":{"begin":867,"end":877},"obj":"Protein"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
bionlp-st-ge-2016-reference
{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T21774","span":{"begin":22,"end":27},"obj":"Protein"},{"id":"T21775","span":{"begin":67,"end":70},"obj":"Protein"},{"id":"T21776","span":{"begin":71,"end":75},"obj":"Protein"},{"id":"T21777","span":{"begin":406,"end":411},"obj":"Protein"},{"id":"T21778","span":{"begin":696,"end":701},"obj":"Protein"},{"id":"T21779","span":{"begin":743,"end":748},"obj":"Protein"},{"id":"T21780","span":{"begin":788,"end":793},"obj":"Protein"},{"id":"T21781","span":{"begin":822,"end":827},"obj":"Protein"},{"id":"T21782","span":{"begin":850,"end":855},"obj":"Protein"},{"id":"T21783","span":{"begin":872,"end":877},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
bionlp-st-ge-2016-uniprot
{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T21956","span":{"begin":22,"end":27},"obj":"Q9BZS1"},{"id":"T21957","span":{"begin":67,"end":70},"obj":"P01730"},{"id":"T21958","span":{"begin":71,"end":75},"obj":"P01589"},{"id":"T21959","span":{"begin":406,"end":411},"obj":"Q9BZS1"},{"id":"T21960","span":{"begin":696,"end":701},"obj":"P23771"},{"id":"T21961","span":{"begin":743,"end":748},"obj":"Q9UL17"},{"id":"T21962","span":{"begin":788,"end":793},"obj":"P04406"},{"id":"T21963","span":{"begin":822,"end":827},"obj":"Q15796"},{"id":"T21964","span":{"begin":850,"end":855},"obj":"P42226"},{"id":"T21965","span":{"begin":872,"end":877},"obj":"P42226"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}
test2
{"project":"test2","denotations":[{"id":"T21764","span":{"begin":22,"end":27},"obj":"Protein"},{"id":"T21765","span":{"begin":67,"end":70},"obj":"Protein"},{"id":"T21766","span":{"begin":71,"end":75},"obj":"Protein"},{"id":"T21767","span":{"begin":406,"end":411},"obj":"Protein"},{"id":"T21768","span":{"begin":696,"end":701},"obj":"Protein"},{"id":"T21769","span":{"begin":743,"end":748},"obj":"Protein"},{"id":"T21770","span":{"begin":788,"end":793},"obj":"Protein"},{"id":"T21771","span":{"begin":822,"end":827},"obj":"Protein"},{"id":"T21772","span":{"begin":850,"end":855},"obj":"Protein"},{"id":"T21773","span":{"begin":872,"end":877},"obj":"Protein"}],"text":"Western blotting.\nFor FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen). The proteins were electroblotted onto a PVDF membrane (Amersham Life Science). Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji). Membranes were incubated in stripping buffer and re-blocked for 1 h. The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),"}