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PMC:2222968 / 43245-44151 JSONTXT

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pmc-enju-pas

Id Subject Object Predicate Lexical cue
T21790 0-7 NN denotes Western
T21791 8-16 NN denotes blotting
T21792 18-21 IN denotes For
T21793 22-27 NN denotes FOXP3
T21794 28-36 NN denotes analysis
T21795 37-39 IN denotes on
T21796 40-43 DT denotes the
T21797 44-51 NN denotes protein
T21798 52-57 NN denotes level
T21799 57-58 -COMMA- denotes ,
T21800 59-60 CD denotes 1
T21801 61-62 CC denotes ×
T21802 63-66 CD denotes 106
T21803 67-76 NN denotes CD4+CD25–
T21804 77-82 NN denotes cells
T21805 83-87 VB denotes were
T21806 88-93 VB denotes lysed
T21807 94-97 CC denotes and
T21808 98-104 VB denotes loaded
T21809 105-109 JJ denotes next
T21810 110-112 TO denotes to
T21811 113-114 DT denotes a
T21812 115-127 JJ denotes protein-mass
T21813 128-134 NN denotes ladder
T21814 135-136 -LRB- denotes (
T21815 136-145 NNP denotes Magicmark
T21816 145-146 -COMMA- denotes ,
T21817 147-157 NN denotes Invitrogen
T21818 157-158 -RRB- denotes )
T21819 159-161 IN denotes on
T21820 162-163 DT denotes a
T21821 164-170 NN denotes NuPAGE
T21822 171-175 CD denotes 4–12
T21823 175-176 NN denotes %
T21824 177-185 JJ denotes bis-tris
T21825 186-189 NN denotes gel
T21826 190-191 -LRB- denotes (
T21827 191-201 NN denotes Invitrogen
T21828 201-202 -RRB- denotes )
T21829 204-207 DT denotes The
T21830 208-216 NN denotes proteins
T21831 217-221 VB denotes were
T21832 222-236 VB denotes electroblotted
T21833 237-241 IN denotes onto
T21834 242-243 DT denotes a
T21835 244-248 NN denotes PVDF
T21836 249-257 NN denotes membrane
T21837 258-259 -LRB- denotes (
T21838 259-267 NNP denotes Amersham
T21839 268-272 NNP denotes Life
T21840 273-280 NNP denotes Science
T21841 280-281 -RRB- denotes )
T21842 283-293 JJ denotes Unspecific
T21843 294-301 NN denotes binding
T21844 302-305 VB denotes was
T21845 306-313 VB denotes blocked
T21846 314-318 IN denotes with
T21847 319-322 NN denotes BSA
T21848 322-323 -COMMA- denotes ,
T21849 324-327 CC denotes and
T21850 328-331 DT denotes the
T21851 332-341 NN denotes membranes
T21852 342-346 VB denotes were
T21853 347-359 RB denotes subsequently
T21854 360-369 VB denotes incubated
T21855 370-374 IN denotes with
T21856 375-377 DT denotes an
T21857 378-379 CD denotes 1
T21858 379-380 -COLON- denotes :
T21859 380-383 CD denotes 200
T21860 384-392 NN denotes dilution
T21861 393-395 IN denotes of
T21862 396-400 NN denotes goat
T21863 401-411 NN denotes anti-FOXP3
T21864 412-414 IN denotes in
T21865 415-423 VB denotes blocking
T21866 424-430 NN denotes buffer
T21867 431-432 -LRB- denotes (
T21868 432-437 NN denotes Abcam
T21869 437-438 -RRB- denotes )
T21870 439-448 RB denotes overnight
T21871 449-451 IN denotes at
T21872 452-453 CD denotes 4
T21873 454-457 NNP denotes °C.
T21874 458-461 DT denotes The
T21875 462-467 NN denotes blots
T21876 468-472 VB denotes were
T21877 473-482 VB denotes developed
T21878 483-488 VB denotes using
T21879 489-491 DT denotes an
T21880 492-501 JJ denotes anti-goat
T21881 502-513 JJ denotes HRP-labeled
T21882 514-517 NN denotes mAb
T21883 518-519 -LRB- denotes (
T21884 519-527 NNP denotes Amersham
T21885 528-539 NNP denotes Biosciences
T21886 539-540 -RRB- denotes )
T21887 541-544 CC denotes and
T21888 545-555 VB denotes visualized
T21889 556-560 IN denotes with
T21890 561-562 DT denotes a
T21891 563-566 NN denotes LAS
T21892 567-571 CD denotes 1000
T21893 572-578 NN denotes camera
T21894 579-580 -LRB- denotes (
T21895 580-584 NN denotes Fuji
T21896 584-585 -RRB- denotes )
T21897 587-596 NN denotes Membranes
T21898 597-601 VB denotes were
T21899 602-611 VB denotes incubated
T21900 612-614 IN denotes in
T21901 615-624 VB denotes stripping
T21902 625-631 NN denotes buffer
T21903 632-635 CC denotes and
T21904 636-646 JJ denotes re-blocked
T21905 647-650 IN denotes for
T21906 651-652 CD denotes 1
T21907 653-654 NN denotes h
T21908 656-659 DT denotes The
T21909 660-669 NN denotes membranes
T21910 670-674 VB denotes were
T21911 675-684 VB denotes re-probed
T21912 685-690 VB denotes using
T21913 691-701 NN denotes anti-GATA3
T21914 702-703 -LRB- denotes (
T21915 703-709 NN denotes HG3–31
T21916 709-710 -COLON- denotes ;
T21917 711-716 NNP denotes Santa
T21918 717-721 NNP denotes Cruz
T21919 722-735 NNP denotes Biotechnology
T21920 735-736 -RRB- denotes )
T21921 736-737 -COMMA- denotes ,
T21922 738-748 JJ denotes anti-T-bet
T21923 749-750 -LRB- denotes (
T21924 750-754 NN denotes 4B10
T21925 754-755 -COMMA- denotes ,
T21926 756-761 NNP denotes Santa
T21927 762-766 NNP denotes Cruz
T21928 767-780 NNP denotes Biotechnology
T21929 780-781 -RRB- denotes )
T21930 781-782 -COMMA- denotes ,
T21931 783-793 NN denotes anti-GAPDH
T21932 794-795 -LRB- denotes (
T21933 795-798 NN denotes 6C5
T21934 798-799 -COMMA- denotes ,
T21935 800-806 NNP denotes Ambion
T21936 806-807 -RRB- denotes )
T21937 807-808 -COMMA- denotes ,
T21938 809-827 NN denotes anti-phospho-SMAD2
T21939 828-829 -LRB- denotes (
T21940 829-834 NN denotes 138D4
T21941 834-835 -RRB- denotes )
T21942 835-836 -COMMA- denotes ,
T21943 837-855 NN denotes anti-phospho-STAT6
T21944 856-857 -LRB- denotes (
T21945 857-860 NN denotes 5A4
T21946 860-861 -RRB- denotes )
T21947 861-862 -COMMA- denotes ,
T21948 863-866 CC denotes and
T21949 867-877 JJ denotes anti-STAT6
T21950 878-879 -LRB- denotes (
T21951 879-883 NNP denotes Cell
T21952 884-893 NNP denotes Signaling
T21953 894-904 NNP denotes Technology
T21954 904-905 -RRB- denotes )
T21955 905-906 -COMMA- denotes ,
R16212 T21791 T21790 arg1Of blotting,Western
R16213 T21794 T21792 arg2Of analysis,For
R16214 T21794 T21793 arg1Of analysis,FOXP3
R16215 T21794 T21795 arg1Of analysis,on
R16216 T21798 T21795 arg2Of level,on
R16217 T21798 T21796 arg1Of level,the
R16218 T21798 T21797 arg1Of level,protein
R16219 T21800 T21801 arg1Of 1,×
R16220 T21802 T21801 arg2Of 106,×
R16221 T21804 T21800 arg1Of cells,1
R16222 T21804 T21802 arg1Of cells,106
R16223 T21804 T21803 arg1Of cells,CD4+CD25–
R16224 T21804 T21805 arg1Of cells,were
R16225 T21804 T21806 arg2Of cells,lysed
R16226 T21804 T21808 arg2Of cells,loaded
R16227 T21806 T21807 arg1Of lysed,and
R16228 T21807 T21792 arg1Of and,For
R16229 T21807 T21799 arg1Of and,","
R16230 T21807 T21805 arg2Of and,were
R16231 T21807 T21819 arg1Of and,on
R16232 T21808 T21807 arg2Of loaded,and
R16233 T21808 T21810 arg1Of loaded,to
R16234 T21808 T21814 arg1Of loaded,(
R16235 T21810 T21809 arg1Of to,next
R16236 T21813 T21810 arg2Of ladder,to
R16237 T21813 T21811 arg1Of ladder,a
R16238 T21813 T21812 arg1Of ladder,protein-mass
R16239 T21815 T21814 arg2Of Magicmark,(
R16240 T21815 T21816 arg1Of Magicmark,","
R16241 T21815 T21817 arg1Of Magicmark,Invitrogen
R16242 T21818 T21814 arg3Of ),(
R16243 T21822 T21823 arg1Of 4–12,%
R16244 T21825 T21819 arg2Of gel,on
R16245 T21825 T21820 arg1Of gel,a
R16246 T21825 T21821 arg1Of gel,NuPAGE
R16247 T21825 T21822 arg1Of gel,4–12
R16248 T21825 T21824 arg1Of gel,bis-tris
R16249 T21825 T21826 arg1Of gel,(
R16250 T21827 T21826 arg2Of Invitrogen,(
R16251 T21828 T21826 arg3Of ),(
R16252 T21830 T21829 arg1Of proteins,The
R16253 T21830 T21831 arg1Of proteins,were
R16254 T21830 T21832 arg2Of proteins,electroblotted
R16255 T21832 T21831 arg2Of electroblotted,were
R16256 T21832 T21833 arg1Of electroblotted,onto
R16257 T21832 T21837 arg1Of electroblotted,(
R16258 T21836 T21833 arg2Of membrane,onto
R16259 T21836 T21834 arg1Of membrane,a
R16260 T21836 T21835 arg1Of membrane,PVDF
R16261 T21840 T21837 arg2Of Science,(
R16262 T21840 T21838 arg1Of Science,Amersham
R16263 T21840 T21839 arg1Of Science,Life
R16264 T21841 T21837 arg3Of ),(
R16265 T21843 T21842 arg1Of binding,Unspecific
R16266 T21843 T21844 arg1Of binding,was
R16267 T21843 T21845 arg2Of binding,blocked
R16268 T21845 T21844 arg2Of blocked,was
R16269 T21845 T21846 arg1Of blocked,with
R16270 T21845 T21849 arg1Of blocked,and
R16271 T21847 T21846 arg2Of BSA,with
R16272 T21849 T21848 arg1Of and,","
R16273 T21849 T21858 arg1Of and,:
R16274 T21851 T21850 arg1Of membranes,the
R16275 T21851 T21852 arg1Of membranes,were
R16276 T21851 T21854 arg2Of membranes,incubated
R16277 T21854 T21849 arg2Of incubated,and
R16278 T21854 T21852 arg2Of incubated,were
R16279 T21854 T21853 arg1Of incubated,subsequently
R16280 T21854 T21855 arg1Of incubated,with
R16281 T21857 T21855 arg2Of 1,with
R16282 T21857 T21856 arg1Of 1,an
R16283 T21860 T21859 arg1Of dilution,200
R16284 T21860 T21861 arg1Of dilution,of
R16285 T21860 T21864 arg1Of dilution,in
R16286 T21860 T21876 arg1Of dilution,were
R16287 T21860 T21877 arg2Of dilution,developed
R16288 T21860 T21888 arg2Of dilution,visualized
R16289 T21863 T21861 arg2Of anti-FOXP3,of
R16290 T21863 T21862 arg1Of anti-FOXP3,goat
R16291 T21865 T21864 arg2Of blocking,in
R16292 T21865 T21870 arg1Of blocking,overnight
R16293 T21865 T21871 arg1Of blocking,at
R16294 T21866 T21865 arg2Of buffer,blocking
R16295 T21866 T21867 arg1Of buffer,(
R16296 T21868 T21867 arg2Of Abcam,(
R16297 T21869 T21867 arg3Of ),(
R16298 T21873 T21872 arg1Of °C.,4
R16299 T21875 T21871 arg2Of blots,at
R16300 T21875 T21873 arg1Of blots,°C.
R16301 T21875 T21874 arg1Of blots,The
R16302 T21877 T21887 arg1Of developed,and
R16303 T21878 T21877 arg3Of using,developed
R16304 T21882 T21878 arg2Of mAb,using
R16305 T21882 T21879 arg1Of mAb,an
R16306 T21882 T21880 arg1Of mAb,anti-goat
R16307 T21882 T21881 arg1Of mAb,HRP-labeled
R16308 T21882 T21883 arg1Of mAb,(
R16309 T21885 T21883 arg2Of Biosciences,(
R16310 T21885 T21884 arg1Of Biosciences,Amersham
R16311 T21886 T21883 arg3Of ),(
R16312 T21887 T21858 arg2Of and,:
R16313 T21887 T21876 arg2Of and,were
R16314 T21888 T21887 arg2Of visualized,and
R16315 T21888 T21889 arg1Of visualized,with
R16316 T21893 T21889 arg2Of camera,with
R16317 T21893 T21890 arg1Of camera,a
R16318 T21893 T21891 arg1Of camera,LAS
R16319 T21893 T21892 arg1Of camera,1000
R16320 T21893 T21894 arg1Of camera,(
R16321 T21895 T21894 arg2Of Fuji,(
R16322 T21896 T21894 arg3Of ),(
R16323 T21897 T21898 arg1Of Membranes,were
R16324 T21897 T21899 arg2Of Membranes,incubated
R16325 T21897 T21904 arg1Of Membranes,re-blocked
R16326 T21899 T21900 arg1Of incubated,in
R16327 T21899 T21903 arg1Of incubated,and
R16328 T21901 T21900 arg2Of stripping,in
R16329 T21902 T21901 arg2Of buffer,stripping
R16330 T21903 T21898 arg2Of and,were
R16331 T21904 T21903 arg2Of re-blocked,and
R16332 T21904 T21905 arg1Of re-blocked,for
R16333 T21907 T21905 arg2Of h,for
R16334 T21907 T21906 arg1Of h,1
R16335 T21909 T21908 arg1Of membranes,The
R16336 T21909 T21910 arg1Of membranes,were
R16337 T21909 T21911 arg2Of membranes,re-probed
R16338 T21911 T21910 arg2Of re-probed,were
R16339 T21911 T21955 arg1Of re-probed,","
R16340 T21912 T21911 arg3Of using,re-probed
R16341 T21913 T21914 arg1Of anti-GATA3,(
R16342 T21913 T21921 arg1Of anti-GATA3,","
R16343 T21915 T21914 arg2Of HG3–31,(
R16344 T21915 T21916 arg1Of HG3–31,;
R16345 T21919 T21916 arg2Of Biotechnology,;
R16346 T21919 T21917 arg1Of Biotechnology,Santa
R16347 T21919 T21918 arg1Of Biotechnology,Cruz
R16348 T21920 T21914 arg3Of ),(
R16349 T21921 T21948 arg1Of ",",and
R16350 T21922 T21923 arg1Of anti-T-bet,(
R16351 T21922 T21930 arg1Of anti-T-bet,","
R16352 T21924 T21923 arg2Of 4B10,(
R16353 T21924 T21925 arg1Of 4B10,","
R16354 T21928 T21925 arg2Of Biotechnology,","
R16355 T21928 T21926 arg1Of Biotechnology,Santa
R16356 T21928 T21927 arg1Of Biotechnology,Cruz
R16357 T21929 T21923 arg3Of ),(
R16358 T21930 T21921 arg2Of ",",","
R16359 T21931 T21932 arg1Of anti-GAPDH,(
R16360 T21931 T21937 arg1Of anti-GAPDH,","
R16361 T21933 T21932 arg2Of 6C5,(
R16362 T21933 T21934 arg1Of 6C5,","
R16363 T21933 T21935 arg1Of 6C5,Ambion
R16364 T21936 T21932 arg3Of ),(
R16365 T21937 T21942 arg1Of ",",","
R16366 T21938 T21937 arg2Of anti-phospho-SMAD2,","
R16367 T21938 T21939 arg1Of anti-phospho-SMAD2,(
R16368 T21940 T21939 arg2Of 138D4,(
R16369 T21941 T21939 arg3Of ),(
R16370 T21942 T21930 arg2Of ",",","
R16371 T21943 T21942 arg2Of anti-phospho-STAT6,","
R16372 T21943 T21944 arg1Of anti-phospho-STAT6,(
R16373 T21945 T21944 arg2Of 5A4,(
R16374 T21946 T21944 arg3Of ),(
R16375 T21948 T21912 arg2Of and,using
R16376 T21948 T21947 arg1Of and,","
R16377 T21949 T21948 arg2Of anti-STAT6,and
R16378 T21949 T21950 arg1Of anti-STAT6,(
R16379 T21953 T21950 arg2Of Technology,(
R16380 T21953 T21951 arg1Of Technology,Cell
R16381 T21953 T21952 arg1Of Technology,Signaling
R16382 T21954 T21950 arg3Of ),(

bionlp-st-ge-2016-spacy-parsed

Id Subject Object Predicate Lexical cue
T21972 0-7 JJ denotes Western
T21973 8-16 VBG denotes blotting
T21974 16-17 . denotes .
T21975 18-21 IN denotes For
T21976 22-27 CD denotes FOXP3
T21977 28-36 NN denotes analysis
T21978 37-39 IN denotes on
T21979 40-43 DT denotes the
T21980 44-51 NN denotes protein
T21981 52-57 NN denotes level
T21982 57-58 , denotes ,
T21983 59-60 CD denotes 1
T21984 61-62 CD denotes ×
T21985 63-66 CD denotes 106
T21986 67-70 NNP denotes CD4
T21987 70-71 NNP denotes +
T21988 71-75 NNP denotes CD25
T21989 77-82 NNS denotes cells
T21990 83-87 VBD denotes were
T21991 88-93 VBN denotes lysed
T21992 94-97 CC denotes and
T21993 98-104 VBN denotes loaded
T21994 105-109 JJ denotes next
T21995 110-112 TO denotes to
T21996 113-114 DT denotes a
T21997 115-127 JJ denotes protein-mass
T21998 128-134 NN denotes ladder
T21999 135-136 -LRB- denotes (
T22000 136-145 NNP denotes Magicmark
T22001 145-146 , denotes ,
T22002 147-157 NNP denotes Invitrogen
T22003 157-158 -RRB- denotes )
T22004 159-161 IN denotes on
T22005 162-163 DT denotes a
T22006 164-170 NNP denotes NuPAGE
T22007 171-172 CD denotes 4
T22008 173-175 CD denotes 12
T22009 175-176 NN denotes %
T22010 177-185 JJ denotes bis-tris
T22011 186-189 NN denotes gel
T22012 190-191 -LRB- denotes (
T22013 191-201 NNP denotes Invitrogen
T22014 201-202 -RRB- denotes )
T22015 202-203 . denotes .
T22016 204-207 DT denotes The
T22017 208-216 NNS denotes proteins
T22018 217-221 VBD denotes were
T22019 222-236 VBN denotes electroblotted
T22020 237-241 IN denotes onto
T22021 242-243 DT denotes a
T22022 244-248 NNP denotes PVDF
T22023 249-257 NN denotes membrane
T22024 258-259 -LRB- denotes (
T22025 259-267 NNP denotes Amersham
T22026 268-272 NNP denotes Life
T22027 273-280 NNP denotes Science
T22028 280-281 -RRB- denotes )
T22029 281-282 . denotes .
T22030 283-293 JJ denotes Unspecific
T22031 294-301 JJ denotes binding
T22032 302-305 VBD denotes was
T22033 306-313 VBN denotes blocked
T22034 314-318 IN denotes with
T22035 319-322 NNP denotes BSA
T22036 322-323 , denotes ,
T22037 324-327 CC denotes and
T22038 328-331 DT denotes the
T22039 332-341 NNS denotes membranes
T22040 342-346 VBD denotes were
T22041 347-359 RB denotes subsequently
T22042 360-369 VBN denotes incubated
T22043 370-374 IN denotes with
T22044 375-377 DT denotes an
T22045 378-383 CD denotes 1:200
T22046 384-392 NN denotes dilution
T22047 393-395 IN denotes of
T22048 396-400 NN denotes goat
T22049 401-411 NN denotes anti-FOXP3
T22050 412-414 IN denotes in
T22051 415-423 VBG denotes blocking
T22052 424-430 NN denotes buffer
T22053 431-432 -LRB- denotes (
T22054 432-437 NNP denotes Abcam
T22055 437-438 -RRB- denotes )
T22056 439-448 RB denotes overnight
T22057 449-451 IN denotes at
T22058 452-453 CD denotes 4
T22059 454-455 CD denotes °
T22060 455-456 NNP denotes C
T22061 456-457 . denotes .
T22062 458-461 DT denotes The
T22063 462-467 NNS denotes blots
T22064 468-472 VBD denotes were
T22065 473-482 VBN denotes developed
T22066 483-488 VBG denotes using
T22067 489-491 DT denotes an
T22068 492-501 JJ denotes anti-goat
T22069 502-513 JJ denotes HRP-labeled
T22070 514-517 NNP denotes mAb
T22071 518-519 -LRB- denotes (
T22072 519-527 NNP denotes Amersham
T22073 528-539 NNPS denotes Biosciences
T22074 539-540 -RRB- denotes )
T22075 541-544 CC denotes and
T22076 545-555 VBN denotes visualized
T22077 556-560 IN denotes with
T22078 561-562 DT denotes a
T22079 563-566 NNP denotes LAS
T22080 567-571 CD denotes 1000
T22081 572-578 NN denotes camera
T22082 579-580 -LRB- denotes (
T22083 580-584 NNP denotes Fuji
T22084 584-585 -RRB- denotes )
T22085 585-586 . denotes .
T22086 587-596 NNS denotes Membranes
T22087 597-601 VBD denotes were
T22088 602-611 VBN denotes incubated
T22089 612-614 IN denotes in
T22090 615-624 VBG denotes stripping
T22091 625-631 NN denotes buffer
T22092 632-635 CC denotes and
T22093 636-646 JJ denotes re-blocked
T22094 647-650 IN denotes for
T22095 651-652 CD denotes 1
T22096 653-654 NN denotes h
T22097 654-655 . denotes .
T22098 656-659 DT denotes The
T22099 660-669 NNS denotes membranes
T22100 670-674 VBD denotes were
T22101 675-684 JJ denotes re-probed
T22102 685-690 VBG denotes using
T22103 691-701 JJ denotes anti-GATA3
T22104 702-703 -LRB- denotes (
T22105 703-706 CD denotes HG3
T22106 707-709 CD denotes 31
T22107 709-710 : denotes ;
T22108 711-716 NNP denotes Santa
T22109 717-721 NNP denotes Cruz
T22110 722-735 NNP denotes Biotechnology
T22111 735-736 -RRB- denotes )
T22112 736-737 , denotes ,
T22113 738-748 JJ denotes anti-T-bet
T22114 749-750 -LRB- denotes (
T22115 750-754 NNP denotes 4B10
T22116 754-755 , denotes ,
T22117 756-761 NNP denotes Santa
T22118 762-766 NNP denotes Cruz
T22119 767-780 NNP denotes Biotechnology
T22120 780-781 -RRB- denotes )
T22121 781-782 , denotes ,
T22122 783-793 JJ denotes anti-GAPDH
T22123 794-795 -LRB- denotes (
T22124 795-798 CD denotes 6C5
T22125 798-799 , denotes ,
T22126 800-806 NNP denotes Ambion
T22127 806-807 -RRB- denotes )
T22128 807-808 , denotes ,
T22129 809-827 JJ denotes anti-phospho-SMAD2
T22130 828-829 -LRB- denotes (
T22131 829-834 CD denotes 138D4
T22132 834-835 -RRB- denotes )
T22133 835-836 , denotes ,
T22134 837-855 JJ denotes anti-phospho-STAT6
T22135 856-857 -LRB- denotes (
T22136 857-860 NNP denotes 5A4
T22137 860-861 -RRB- denotes )
T22138 861-862 , denotes ,
T22139 863-866 CC denotes and
T22140 867-877 NNP denotes anti-STAT6
T22141 878-879 -LRB- denotes (
T22142 879-883 NNP denotes Cell
T22143 884-893 NNP denotes Signaling
T22144 894-904 NNP denotes Technology
T22145 904-905 -RRB- denotes )
T22146 905-906 , denotes ,
R16383 T21972 T21973 amod Western,blotting
R16384 T21973 T21973 ROOT blotting,blotting
R16385 T21974 T21973 punct .,blotting
R16386 T21975 T21991 prep For,lysed
R16387 T21976 T21977 nummod FOXP3,analysis
R16388 T21977 T21975 pobj analysis,For
R16389 T21978 T21977 prep on,analysis
R16390 T21979 T21981 det the,level
R16391 T21980 T21981 compound protein,level
R16392 T21981 T21978 pobj level,on
R16393 T21982 T21991 punct ",",lysed
R16394 T21983 T21985 nummod 1,106
R16395 T21984 T21985 nummod ×,106
R16396 T21985 T21988 nummod 106,CD25
R16397 T21986 T21988 compound CD4,CD25
R16398 T21987 T21988 compound +,CD25
R16399 T21988 T21991 npadvmod CD25,lysed
R16400 T21989 T21991 nsubjpass cells,lysed
R16401 T21990 T21991 auxpass were,lysed
R16402 T21991 T21991 ROOT lysed,lysed
R16403 T21992 T21991 cc and,lysed
R16404 T21993 T21991 conj loaded,lysed
R16405 T21994 T21993 advmod next,loaded
R16406 T21995 T21994 prep to,next
R16407 T21996 T21998 det a,ladder
R16408 T21997 T21998 amod protein-mass,ladder
R16409 T21998 T21995 pobj ladder,to
R16410 T21999 T21998 punct (,ladder
R16411 T22000 T21998 appos Magicmark,ladder
R16412 T22001 T22000 punct ",",Magicmark
R16413 T22002 T22000 appos Invitrogen,Magicmark
R16414 T22003 T22000 punct ),Magicmark
R16415 T22004 T21998 prep on,ladder
R16416 T22005 T22011 det a,gel
R16417 T22006 T22011 nmod NuPAGE,gel
R16418 T22007 T22006 nummod 4,NuPAGE
R16419 T22008 T22009 nummod 12,%
R16420 T22009 T22011 nmod %,gel
R16421 T22010 T22011 amod bis-tris,gel
R16422 T22011 T22004 pobj gel,on
R16423 T22012 T22013 punct (,Invitrogen
R16424 T22013 T22011 appos Invitrogen,gel
R16425 T22014 T21993 punct ),loaded
R16426 T22015 T21991 punct .,lysed
R16427 T22016 T22017 det The,proteins
R16428 T22017 T22019 nsubjpass proteins,electroblotted
R16429 T22018 T22019 auxpass were,electroblotted
R16430 T22019 T22019 ROOT electroblotted,electroblotted
R16431 T22020 T22019 prep onto,electroblotted
R16432 T22021 T22023 det a,membrane
R16433 T22022 T22023 compound PVDF,membrane
R16434 T22023 T22020 pobj membrane,onto
R16435 T22024 T22027 punct (,Science
R16436 T22025 T22027 compound Amersham,Science
R16437 T22026 T22027 compound Life,Science
R16438 T22027 T22023 appos Science,membrane
R16439 T22028 T22019 punct ),electroblotted
R16440 T22029 T22019 punct .,electroblotted
R16441 T22030 T22031 amod Unspecific,binding
R16442 T22031 T22033 nsubjpass binding,blocked
R16443 T22032 T22033 auxpass was,blocked
R16444 T22033 T22033 ROOT blocked,blocked
R16445 T22034 T22033 prep with,blocked
R16446 T22035 T22034 pobj BSA,with
R16447 T22036 T22033 punct ",",blocked
R16448 T22037 T22033 cc and,blocked
R16449 T22038 T22039 det the,membranes
R16450 T22039 T22042 nsubjpass membranes,incubated
R16451 T22040 T22042 auxpass were,incubated
R16452 T22041 T22042 advmod subsequently,incubated
R16453 T22042 T22033 conj incubated,blocked
R16454 T22043 T22042 prep with,incubated
R16455 T22044 T22046 det an,dilution
R16456 T22045 T22046 nummod 1:200,dilution
R16457 T22046 T22043 pobj dilution,with
R16458 T22047 T22046 prep of,dilution
R16459 T22048 T22049 compound goat,anti-FOXP3
R16460 T22049 T22047 pobj anti-FOXP3,of
R16461 T22050 T22046 prep in,dilution
R16462 T22051 T22050 pcomp blocking,in
R16463 T22052 T22051 dobj buffer,blocking
R16464 T22053 T22052 punct (,buffer
R16465 T22054 T22052 appos Abcam,buffer
R16466 T22055 T22052 punct ),buffer
R16467 T22056 T22051 npadvmod overnight,blocking
R16468 T22057 T22051 prep at,blocking
R16469 T22058 T22059 nummod 4,°
R16470 T22059 T22057 pobj °,at
R16471 T22060 T22050 pobj C,in
R16472 T22061 T22042 punct .,incubated
R16473 T22062 T22063 det The,blots
R16474 T22063 T22065 nsubjpass blots,developed
R16475 T22064 T22065 auxpass were,developed
R16476 T22065 T22065 ROOT developed,developed
R16477 T22066 T22065 advcl using,developed
R16478 T22067 T22070 det an,mAb
R16479 T22068 T22070 amod anti-goat,mAb
R16480 T22069 T22070 compound HRP-labeled,mAb
R16481 T22070 T22066 dobj mAb,using
R16482 T22071 T22073 punct (,Biosciences
R16483 T22072 T22073 compound Amersham,Biosciences
R16484 T22073 T22070 parataxis Biosciences,mAb
R16485 T22074 T22073 punct ),Biosciences
R16486 T22075 T22066 cc and,using
R16487 T22076 T22066 conj visualized,using
R16488 T22077 T22076 prep with,visualized
R16489 T22078 T22081 det a,camera
R16490 T22079 T22081 nmod LAS,camera
R16491 T22080 T22081 nummod 1000,camera
R16492 T22081 T22077 pobj camera,with
R16493 T22082 T22081 punct (,camera
R16494 T22083 T22081 appos Fuji,camera
R16495 T22084 T22081 punct ),camera
R16496 T22085 T22065 punct .,developed
R16497 T22086 T22088 nsubjpass Membranes,incubated
R16498 T22087 T22088 auxpass were,incubated
R16499 T22088 T22088 ROOT incubated,incubated
R16500 T22089 T22088 prep in,incubated
R16501 T22090 T22089 pcomp stripping,in
R16502 T22091 T22094 meta buffer,for
R16503 T22092 T22091 cc and,buffer
R16504 T22093 T22091 conj re-blocked,buffer
R16505 T22094 T22090 prep for,stripping
R16506 T22095 T22096 nummod 1,h
R16507 T22096 T22094 pobj h,for
R16508 T22097 T22088 punct .,incubated
R16509 T22098 T22099 det The,membranes
R16510 T22099 T22100 nsubj membranes,were
R16511 T22100 T22100 ROOT were,were
R16512 T22101 T22110 dep re-probed,Biotechnology
R16513 T22102 T22101 advcl using,re-probed
R16514 T22103 T22102 dobj anti-GATA3,using
R16515 T22104 T22103 punct (,anti-GATA3
R16516 T22105 T22103 appos HG3,anti-GATA3
R16517 T22106 T22103 nummod 31,anti-GATA3
R16518 T22107 T22110 punct ;,Biotechnology
R16519 T22108 T22109 compound Santa,Cruz
R16520 T22109 T22110 compound Cruz,Biotechnology
R16521 T22110 T22100 acomp Biotechnology,were
R16522 T22111 T22110 punct ),Biotechnology
R16523 T22112 T22110 punct ",",Biotechnology
R16524 T22113 T22110 conj anti-T-bet,Biotechnology
R16525 T22114 T22129 punct (,anti-phospho-SMAD2
R16526 T22115 T22129 nmod 4B10,anti-phospho-SMAD2
R16527 T22116 T22115 punct ",",4B10
R16528 T22117 T22118 compound Santa,Cruz
R16529 T22118 T22119 compound Cruz,Biotechnology
R16530 T22119 T22115 appos Biotechnology,4B10
R16531 T22120 T22115 punct ),4B10
R16532 T22121 T22115 punct ",",4B10
R16533 T22122 T22115 conj anti-GAPDH,4B10
R16534 T22123 T22122 punct (,anti-GAPDH
R16535 T22124 T22122 appos 6C5,anti-GAPDH
R16536 T22125 T22122 punct ",",anti-GAPDH
R16537 T22126 T22122 appos Ambion,anti-GAPDH
R16538 T22127 T22122 punct ),anti-GAPDH
R16539 T22128 T22122 punct ",",anti-GAPDH
R16540 T22129 T22113 appos anti-phospho-SMAD2,anti-T-bet
R16541 T22130 T22129 punct (,anti-phospho-SMAD2
R16542 T22131 T22129 appos 138D4,anti-phospho-SMAD2
R16543 T22132 T22129 punct ),anti-phospho-SMAD2
R16544 T22133 T22129 punct ",",anti-phospho-SMAD2
R16545 T22134 T22129 appos anti-phospho-STAT6,anti-phospho-SMAD2
R16546 T22135 T22136 punct (,5A4
R16547 T22136 T22134 appos 5A4,anti-phospho-STAT6
R16548 T22137 T22136 punct ),5A4
R16549 T22138 T22134 punct ",",anti-phospho-STAT6
R16550 T22139 T22134 cc and,anti-phospho-STAT6
R16551 T22140 T22134 conj anti-STAT6,anti-phospho-STAT6
R16552 T22141 T22144 punct (,Technology
R16553 T22142 T22144 compound Cell,Technology
R16554 T22143 T22144 compound Signaling,Technology
R16555 T22144 T22140 parataxis Technology,anti-STAT6
R16556 T22145 T22144 punct ),Technology
R16557 T22146 T22110 punct ",",Biotechnology

GO-MF

Id Subject Object Predicate Lexical cue
T22157 294-301 http://purl.obolibrary.org/obo/GO_0005488 denotes binding

GO-CC

Id Subject Object Predicate Lexical cue
T22158 77-82 http://purl.obolibrary.org/obo/GO_0005623 denotes cells
T22159 249-257 http://purl.obolibrary.org/obo/GO_0016020 denotes membrane
T22160 332-341 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes
T22161 660-669 http://purl.obolibrary.org/obo/GO_0016020 denotes membranes

sentences

Id Subject Object Predicate Lexical cue
T21784 0-17 Sentence denotes Western blotting.
T21785 18-203 Sentence denotes For FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T21786 204-282 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Life Science).
T21787 283-586 Sentence denotes Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C. The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji).
T21788 587-655 Sentence denotes Membranes were incubated in stripping buffer and re-blocked for 1 h.
T21789 656-906 Sentence denotes The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),
T323 0-17 Sentence denotes Western blotting.
T324 18-203 Sentence denotes For FOXP3 analysis on the protein level, 1 × 106 CD4+CD25– cells were lysed and loaded next to a protein-mass ladder (Magicmark, Invitrogen) on a NuPAGE 4–12% bis-tris gel (Invitrogen).
T325 204-282 Sentence denotes The proteins were electroblotted onto a PVDF membrane (Amersham Life Science).
T326 283-457 Sentence denotes Unspecific binding was blocked with BSA, and the membranes were subsequently incubated with an 1:200 dilution of goat anti-FOXP3 in blocking buffer (Abcam) overnight at 4 °C.
T327 458-586 Sentence denotes The blots were developed using an anti-goat HRP-labeled mAb (Amersham Biosciences) and visualized with a LAS 1000 camera (Fuji).
T328 587-655 Sentence denotes Membranes were incubated in stripping buffer and re-blocked for 1 h.
T329 656-906 Sentence denotes The membranes were re-probed using anti-GATA3 (HG3–31; Santa Cruz Biotechnology), anti-T-bet (4B10, Santa Cruz Biotechnology), anti-GAPDH (6C5, Ambion), anti-phospho-SMAD2 (138D4), anti-phospho-STAT6 (5A4), and anti-STAT6 (Cell Signaling Technology),

events-check-again

Id Subject Object Predicate Lexical cue
T22201 22-27 Protein denotes FOXP3
T22202 67-70 Protein denotes CD4
T22203 71-75 Protein denotes CD25
T22204 406-411 Protein denotes FOXP3
T22205 696-701 Protein denotes GATA3
T22206 743-748 Protein denotes T-bet
T22207 788-793 Protein denotes GAPDH
T22208 822-827 Protein denotes SMAD2
T22209 850-855 Protein denotes STAT6
T22210 872-877 Protein denotes STAT6

bionlp-st-ge-2016-reference-tees

Id Subject Object Predicate Lexical cue
T22162 22-27 Protein denotes FOXP3
T22163 67-70 Protein denotes CD4
T22164 71-75 Protein denotes CD25
T22165 396-411 Protein denotes goat anti-FOXP3
T22166 514-517 Protein denotes mAb
T22167 867-877 Protein denotes anti-STAT6

bionlp-st-ge-2016-reference

Id Subject Object Predicate Lexical cue
T21774 22-27 Protein denotes FOXP3
T21775 67-70 Protein denotes CD4
T21776 71-75 Protein denotes CD25
T21777 406-411 Protein denotes FOXP3
T21778 696-701 Protein denotes GATA3
T21779 743-748 Protein denotes T-bet
T21780 788-793 Protein denotes GAPDH
T21781 822-827 Protein denotes SMAD2
T21782 850-855 Protein denotes STAT6
T21783 872-877 Protein denotes STAT6

bionlp-st-ge-2016-uniprot

Id Subject Object Predicate Lexical cue
T21956 22-27 Q9BZS1 denotes FOXP3
T21957 67-70 P01730 denotes CD4
T21958 71-75 P01589 denotes CD25
T21959 406-411 Q9BZS1 denotes FOXP3
T21960 696-701 P23771 denotes GATA3
T21961 743-748 Q9UL17 denotes T-bet
T21962 788-793 P04406 denotes GAPDH
T21963 822-827 Q15796 denotes SMAD2
T21964 850-855 P42226 denotes STAT6
T21965 872-877 P42226 denotes STAT6

test2

Id Subject Object Predicate Lexical cue
T21764 22-27 Protein denotes FOXP3
T21765 67-70 Protein denotes CD4
T21766 71-75 Protein denotes CD25
T21767 406-411 Protein denotes FOXP3
T21768 696-701 Protein denotes GATA3
T21769 743-748 Protein denotes T-bet
T21770 788-793 Protein denotes GAPDH
T21771 822-827 Protein denotes SMAD2
T21772 850-855 Protein denotes STAT6
T21773 872-877 Protein denotes STAT6