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After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"UBERON-AE","denotations":[{"id":"T19754","span":{"begin":263,"end":268},"obj":"http://purl.obolibrary.org/obo/UBERON_0001977"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"GO-BP","denotations":[{"id":"T19765","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0045289"},{"id":"T19766","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0047077"},{"id":"T19767","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0047712"},{"id":"T19768","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0050248"},{"id":"T19769","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0050397"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"GO-MF","denotations":[{"id":"T20001","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0045289"},{"id":"T20002","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0047077"},{"id":"T20003","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0047712"},{"id":"T20004","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0050248"},{"id":"T20005","span":{"begin":326,"end":345},"obj":"http://purl.obolibrary.org/obo/GO_0050397"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"GO-CC","denotations":[{"id":"T20007","span":{"begin":104,"end":109},"obj":"http://purl.obolibrary.org/obo/GO_0005623"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"sentences","denotations":[{"id":"T19763","span":{"begin":0,"end":458},"obj":"Sentence"},{"id":"T293","span":{"begin":0,"end":238},"obj":"Sentence"},{"id":"T294","span":{"begin":239,"end":458},"obj":"Sentence"}],"namespaces":[{"prefix":"_base","uri":"http://pubannotation.org/ontology/tao.owl#"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"events-check-again","denotations":[{"id":"T20030","span":{"begin":14,"end":19},"obj":"Protein"},{"id":"T20031","span":{"begin":29,"end":39},"obj":"Protein"},{"id":"T20032","span":{"begin":97,"end":100},"obj":"Protein"},{"id":"T20033","span":{"begin":326,"end":336},"obj":"Protein"},{"id":"T20034","span":{"begin":371,"end":381},"obj":"Protein"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"bionlp-st-ge-2016-reference-tees","denotations":[{"id":"T20008","span":{"begin":14,"end":48},"obj":"Protein"},{"id":"T20009","span":{"begin":326,"end":336},"obj":"Protein"},{"id":"T20010","span":{"begin":371,"end":381},"obj":"Protein"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"bionlp-st-ge-2016-reference","denotations":[{"id":"T19755","span":{"begin":14,"end":19},"obj":"Protein"},{"id":"T19756","span":{"begin":29,"end":39},"obj":"Protein"},{"id":"T19757","span":{"begin":97,"end":100},"obj":"Protein"},{"id":"T19758","span":{"begin":326,"end":336},"obj":"Protein"},{"id":"T19759","span":{"begin":371,"end":381},"obj":"Protein"}],"namespaces":[{"prefix":"_base","uri":"http://bionlp.dbcls.jp/ontology/ge.owl#"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"bionlp-st-ge-2016-uniprot","denotations":[{"id":"T19875","span":{"begin":14,"end":19},"obj":"Q9BZS1"},{"id":"T19876","span":{"begin":29,"end":39},"obj":"P08659"},{"id":"T19877","span":{"begin":97,"end":100},"obj":"P01730"},{"id":"T19878","span":{"begin":326,"end":336},"obj":"P08659"},{"id":"T19879","span":{"begin":371,"end":381},"obj":"P08659"}],"namespaces":[{"prefix":"_base","uri":"http://www.uniprot.org/uniprot/"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}

{"project":"test2","denotations":[{"id":"T19747","span":{"begin":14,"end":19},"obj":"Protein"},{"id":"T19748","span":{"begin":29,"end":39},"obj":"Protein"},{"id":"T19749","span":{"begin":97,"end":100},"obj":"Protein"},{"id":"T19750","span":{"begin":326,"end":336},"obj":"Protein"},{"id":"T19751","span":{"begin":371,"end":381},"obj":"Protein"}],"text":"3.5 μg of the FOXP3 promoter luciferase reporter vector and 0.5 μg phRL-TK were added to 3 × 106 CD4+ T cells resuspended in 100 μL of Nucleofector solution (Amaxa Biosystems) and electroporated using the U-15 program of the Nucleofector. After a 24-h culture in serum-free conditions and stimuli as indicated in the figures, luciferase activity was measured by the dual luciferase assay system (Promega Biotech) according to the manufacturer's instructions."}